AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG009962

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009962 MtrunA17_Chr2g0319091 98.446 579 9 0 1 579 1 579 0.0 1186
MsaG009962 MtrunA17_Chr5g0447691 64.083 387 136 1 195 578 157 543 0.0 527
MsaG009962 MtrunA17_Chr3g0101141 58.871 372 153 0 207 578 170 541 2.48e-169 492
MsaG009962 MtrunA17_Chr5g0445331 49.703 505 208 6 79 578 68 531 3.27e-167 486
MsaG009962 MtrunA17_Chr4g0076091 48.723 470 188 3 117 578 118 542 5.90e-158 462
MsaG009962 MtrunA17_Chr1g0159461 43.785 539 282 7 44 578 70 591 3.19e-147 437
MsaG009962 MtrunA17_Chr2g0290241 53.439 378 169 2 205 578 50 424 5.43e-144 423
MsaG009962 MtrunA17_Chr3g0125811 53.243 370 173 0 210 579 201 570 9.99e-144 427
MsaG009962 MtrunA17_Chr7g0224271 34.592 503 274 9 113 578 188 672 3.42e-79 263
MsaG009962 MtrunA17_Chr4g0031711 33.511 376 245 3 208 578 357 732 7.88e-70 239
MsaG009962 MtrunA17_Chr4g0031721 32.632 380 253 2 202 578 304 683 3.11e-68 234
MsaG009962 MtrunA17_Chr2g0328391 33.511 376 243 4 208 578 295 668 2.91e-67 231
MsaG009962 MtrunA17_Chr7g0236851 36.533 375 222 6 210 578 292 656 2.11e-66 228
MsaG009962 MtrunA17_Chr2g0306641 36.410 390 225 9 195 578 114 486 7.12e-66 223
MsaG009962 MtrunA17_Chr4g0031701 29.583 551 351 11 40 577 98 624 1.08e-65 226
MsaG009962 MtrunA17_Chr4g0031731 28.962 549 357 9 40 577 98 624 1.17e-65 225
MsaG009962 MtrunA17_Chr8g0357601 35.401 387 223 7 208 579 178 552 2.16e-63 218
MsaG009962 MtrunA17_Chr6g0468561 36.623 385 214 10 210 579 293 662 1.33e-62 218
MsaG009962 MtrunA17_Chr3g0110971 32.373 451 251 11 148 578 170 586 1.66e-62 216
MsaG009962 MtrunA17_Chr4g0057891 31.854 383 239 5 209 578 146 519 3.10e-62 214
MsaG009962 MtrunA17_Chr8g0376381 36.170 376 206 12 213 578 170 521 4.19e-62 214
MsaG009962 MtrunA17_Chr7g0238891 31.720 372 253 1 208 578 368 739 6.25e-61 215
MsaG009962 MtrunA17_Chr4g0031741 32.440 373 247 4 208 578 280 649 4.97e-60 211
MsaG009962 MtrunA17_Chr7g0245601 35.422 367 216 7 216 578 442 791 5.19e-60 213
MsaG009962 MtrunA17_Chr2g0328381 32.095 377 243 4 208 578 271 640 1.90e-59 209
MsaG009962 MtrunA17_Chr2g0328341 31.016 374 251 3 208 578 268 637 6.54e-58 204
MsaG009962 MtrunA17_Chr2g0328431 32.048 415 269 7 166 578 250 653 9.77e-57 202
MsaG009962 MtrunA17_Chr2g0328411 31.948 385 258 3 195 578 272 653 3.13e-56 201
MsaG009962 MtrunA17_Chr3g0085681 30.769 416 232 6 213 578 26 435 3.49e-56 195
MsaG009962 MtrunA17_Chr4g0038851 31.395 430 229 8 209 578 45 468 5.09e-56 196
MsaG009962 MtrunA17_Chr4g0039661 37.057 367 203 12 225 578 203 554 2.21e-53 191
MsaG009962 MtrunA17_Chr5g0401131 31.937 382 239 8 212 578 114 489 1.43e-51 184
MsaG009962 MtrunA17_Chr4g0038761 34.771 371 219 10 216 579 88 442 7.78e-51 181
MsaG009962 MtrunA17_Chr4g0057291 31.959 388 233 11 208 578 288 661 1.59e-50 185
MsaG009962 MtrunA17_Chr2g0328401 30.250 400 270 5 163 561 245 636 3.07e-48 178
MsaG009962 MtrunA17_Chr1g0183641 32.546 381 224 12 208 578 119 476 6.38e-48 174
MsaG009962 MtrunA17_Chr2g0328361 30.053 376 227 8 208 578 270 614 7.16e-47 174
MsaG009962 MtrunA17_Chr3g0085361 32.448 339 173 6 289 578 18 349 7.69e-47 167
MsaG009962 MtrunA17_Chr1g0200401 29.247 465 255 17 162 579 22 459 1.31e-42 159
MsaG009962 MtrunA17_Chr3g0114841 31.818 396 231 13 209 578 117 499 2.76e-41 156
MsaG009962 MtrunA17_Chr2g0329591 29.124 388 245 8 211 578 73 450 5.40e-41 154
MsaG009962 MtrunA17_Chr5g0424131 30.928 388 243 10 209 578 118 498 5.57e-40 152
MsaG009962 MtrunA17_Chr2g0328371 33.333 240 155 2 293 531 12 247 1.45e-38 142
MsaG009962 MtrunA17_Chr5g0401421 25.527 427 248 11 209 578 32 445 2.17e-33 132
MsaG009962 MtrunA17_Chr2g0294461 25.831 391 256 10 209 578 207 584 5.66e-32 130
MsaG009962 MtrunA17_Chr5g0396491 34.762 210 130 2 209 417 53 256 1.78e-31 127
MsaG009962 MtrunA17_Chr5g0396491 42.282 149 85 1 431 578 329 477 1.01e-29 122
MsaG009962 MtrunA17_Chr4g0053901 24.197 467 299 9 166 578 2 467 1.48e-30 125
MsaG009962 MtrunA17_Chr2g0294471 25.131 382 267 9 209 578 201 575 1.40e-27 117
MsaG009962 MtrunA17_Chr7g0270031 24.545 440 289 13 151 578 145 553 5.70e-27 115
MsaG009962 MtrunA17_Chr8g0383981 27.543 403 246 17 190 578 125 495 6.61e-27 114
MsaG009962 MtrunA17_Chr7g0230771 26.042 384 268 8 197 578 361 730 9.14e-27 115
MsaG009962 MtrunA17_Chr7g0230731 25.260 384 271 7 197 578 358 727 1.27e-25 111
MsaG009962 MtrunA17_Chr4g0012071 24.786 351 222 9 243 578 227 550 2.36e-25 110
MsaG009962 MtrunA17_Chr1g0194621 25.424 354 231 12 243 578 179 517 5.14e-24 105
MsaG009962 MtrunA17_Chr2g0323111 25.862 174 127 2 406 578 133 305 6.86e-16 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009962 AT1G50600.3 63.061 379 140 0 200 578 147 525 4.31e-176 509
MsaG009962 AT1G50600.1 63.061 379 140 0 200 578 218 596 1.39e-175 510
MsaG009962 AT1G50600.2 63.061 379 140 0 200 578 222 600 1.41e-175 510
MsaG009962 AT5G48150.1 62.205 381 143 1 199 578 109 489 4.55e-171 494
MsaG009962 AT5G48150.4 62.205 381 143 1 199 578 109 489 4.55e-171 494
MsaG009962 AT5G48150.2 62.205 381 143 1 199 578 109 489 4.55e-171 494
MsaG009962 AT5G48150.3 62.205 381 143 1 199 578 109 489 4.55e-171 494
MsaG009962 AT2G04890.1 60.317 378 143 1 201 578 42 412 1.77e-164 475
MsaG009962 AT4G17230.1 53.806 381 176 0 198 578 144 524 6.49e-145 429
MsaG009962 AT4G17230.2 53.806 381 176 0 198 578 144 524 6.49e-145 429
MsaG009962 AT1G21450.1 46.039 467 227 7 114 579 151 593 1.04e-137 413
MsaG009962 AT1G07530.1 33.600 375 247 2 206 578 390 764 9.01e-70 240
MsaG009962 AT5G52510.1 35.844 385 222 6 210 579 266 640 1.70e-66 228
MsaG009962 AT2G29060.2 33.867 375 244 3 208 578 280 654 1.25e-65 226
MsaG009962 AT2G29060.1 33.867 375 244 3 208 578 317 691 1.98e-65 226
MsaG009962 AT3G03450.1 35.027 374 230 7 209 578 180 544 7.26e-65 222
MsaG009962 AT1G66350.1 34.667 375 219 9 209 578 152 505 8.24e-64 218
MsaG009962 AT5G17490.1 33.957 374 228 8 209 578 157 515 1.64e-62 215
MsaG009962 AT3G46600.2 34.140 372 241 4 209 578 1 370 1.22e-61 208
MsaG009962 AT3G46600.1 34.316 373 241 4 208 578 208 578 2.29e-61 213
MsaG009962 AT3G46600.3 34.316 373 241 4 208 578 176 546 2.67e-61 212
MsaG009962 AT5G59450.1 32.626 377 246 4 208 578 223 597 1.21e-60 212
MsaG009962 AT1G14920.1 35.544 377 219 10 209 578 169 528 2.93e-60 209
MsaG009962 AT1G50420.1 31.323 431 229 5 209 578 54 478 4.79e-60 207
MsaG009962 AT3G54220.1 34.383 413 250 9 172 578 252 649 6.63e-60 211
MsaG009962 AT2G01570.1 33.862 378 224 9 209 578 221 580 1.14e-57 203
MsaG009962 AT2G37650.2 31.720 372 251 2 208 578 331 700 1.27e-57 205
MsaG009962 AT2G37650.1 31.720 372 251 2 208 578 343 712 1.48e-57 205
MsaG009962 AT1G07520.2 31.316 380 251 6 208 578 150 528 1.42e-56 199
MsaG009962 AT1G07520.4 31.316 380 251 6 208 578 150 528 1.42e-56 199
MsaG009962 AT1G07520.1 31.579 380 250 6 208 578 314 692 4.49e-56 201
MsaG009962 AT1G07520.3 31.316 380 251 6 208 578 315 693 5.79e-56 201
MsaG009962 AT5G66770.1 34.383 381 227 10 209 579 217 584 1.20e-53 192
MsaG009962 AT2G29065.1 31.234 381 251 6 208 578 248 627 1.04e-52 191
MsaG009962 AT3G50650.1 34.635 384 226 10 208 579 172 542 1.15e-51 186
MsaG009962 AT1G55580.1 34.304 309 179 6 285 578 145 444 1.93e-49 177
MsaG009962 AT5G41920.1 31.738 397 245 8 191 578 20 399 3.87e-48 173
MsaG009962 AT4G37650.1 30.102 392 240 10 212 578 146 528 2.38e-47 174
MsaG009962 AT1G63100.1 31.877 389 239 8 208 578 248 628 1.00e-46 174
MsaG009962 AT1G63100.2 31.877 389 239 8 208 578 273 653 1.23e-46 174
MsaG009962 AT4G08250.1 28.198 383 252 10 209 578 104 476 7.57e-34 135
MsaG009962 AT3G49950.1 24.031 387 271 9 209 578 27 407 8.71e-30 122
MsaG009962 AT2G45160.1 26.171 363 218 12 237 578 305 638 8.17e-24 106
MsaG009962 AT3G60630.1 27.723 303 188 8 291 578 335 621 6.61e-23 103
MsaG009962 AT3G13840.1 25.109 458 301 14 135 578 80 509 1.24e-20 95.9
MsaG009962 AT5G67411.1 34.821 112 71 2 405 516 89 198 1.83e-11 64.3

Find 151 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGTCCCTACTTAAAATT+TGG 0.130718 2:-67129722 MsaT009962.1:CDS
AAAGATGGGGCAATGCTCTT+AGG 0.253125 2:-67128921 MsaT009962.1:CDS
CTTCAGGCTCTCTGCACTTA+AGG 0.286477 2:+67129783 None:intergenic
CCAGAGGCTAGGTGCTTATA+TGG 0.309502 2:-67129858 MsaT009962.1:CDS
TCACTGCCAAATGGTTTGTT+TGG 0.328575 2:-67130526 MsaT009962.1:CDS
TTCAAGTGATGAGTTATAAG+AGG 0.330718 2:+67130372 None:intergenic
TACCACATCTAATTCTTCAT+TGG 0.339637 2:-67130188 MsaT009962.1:CDS
TCCACAAGGGTAACCACTTT+AGG 0.342806 2:+67129272 None:intergenic
GGCATCCCGGTTGAGTTTCA+TGG 0.352118 2:-67129458 MsaT009962.1:CDS
GTGAGTAATTCTTCACCTTC+AGG 0.364548 2:+67129767 None:intergenic
AGAATGATGTTTGCGTATAA+TGG 0.372625 2:+67130344 None:intergenic
GCTTTGATGTCTAAGAAATT+CGG 0.375367 2:-67129479 MsaT009962.1:CDS
TAATGTGGAGCAGCATTGTT+TGG 0.392234 2:-67129129 MsaT009962.1:CDS
GTTTCCAAAGCCAACTTTCA+AGG 0.393842 2:+67130501 None:intergenic
TTCTAGCCGTTTCTATCAAT+CGG 0.394967 2:+67129909 None:intergenic
TGCTTCGGCAATGGCTCCAT+TGG 0.397586 2:+67129688 None:intergenic
CTACTTAAAATTTGGTTACA+TGG 0.400326 2:-67129714 MsaT009962.1:CDS
AGCATGTCTCTTGTCACATC+TGG 0.401716 2:+67129419 None:intergenic
ACACAACACCTTTCTTCAAC+AGG 0.402167 2:-67129232 MsaT009962.1:CDS
ACCACATCTAATTCTTCATT+GGG 0.403891 2:-67130187 MsaT009962.1:CDS
TTCAGGCTCTCTGCACTTAA+GGG 0.407636 2:+67129784 None:intergenic
GGATCCTTGAAAGTTGGCTT+TGG 0.411943 2:-67130505 MsaT009962.1:CDS
ATCCTCATTCCTGCATGCTT+CGG 0.417086 2:+67129673 None:intergenic
GTTGCAAGGACGGAAGCATC+AGG 0.425786 2:-67129824 MsaT009962.1:CDS
TATCAGACACGGAGAAGCTT+TGG 0.432770 2:-67129393 MsaT009962.1:CDS
TCCTAAAGTGGTTACCCTTG+TGG 0.433600 2:-67129273 MsaT009962.1:CDS
GAAAATCCGACACGCCTTGT+TGG 0.433986 2:-67130251 MsaT009962.1:CDS
AAGGATCCAAACAAACCATT+TGG 0.437989 2:+67130520 None:intergenic
ATTTGATTGCCTGCTACTGC+TGG 0.442739 2:+67130066 None:intergenic
CTCCTTGCTATTTCTTGAGA+GGG 0.443191 2:+67129157 None:intergenic
GTCACATCTGGACCGAAAAC+AGG 0.445280 2:+67129431 None:intergenic
GGTATCCCATGAAACTCAAC+CGG 0.448710 2:+67129452 None:intergenic
ATGAGAGGAAAACGGTGAAT+CGG 0.450658 2:+67130474 None:intergenic
TTGCTCCATGATCTATATCT+CGG 0.451721 2:+67130136 None:intergenic
GAAATGCTGTGTCTATCAAT+GGG 0.460974 2:-67129889 MsaT009962.1:CDS
GTGGTAACTTCGTTATCATC+GGG 0.461161 2:+67130205 None:intergenic
AGAAATGCTGTGTCTATCAA+TGG 0.466776 2:-67129890 MsaT009962.1:CDS
AAGATGGGGCAATGCTCTTA+GGG 0.467770 2:-67128920 MsaT009962.1:CDS
CGCACGTGGGGTGCCCCACC+AGG 0.468298 2:+67129575 None:intergenic
TGAGAGGAAAACGGTGAATC+GGG 0.469261 2:+67130475 None:intergenic
AACAAACCATTTGGCAGTGA+TGG 0.470630 2:+67130529 None:intergenic
CCATATAAGCACCTAGCCTC+TGG 0.472152 2:+67129858 None:intergenic
TGTAGAGAAACGGCGGAAGT+TGG 0.473337 2:-67130032 MsaT009962.1:CDS
AAGTCTAGGTTGACAATGGC+TGG 0.474170 2:-67129032 MsaT009962.1:CDS
TCTATGAACCTGTTGAAGAA+AGG 0.480955 2:+67129224 None:intergenic
CAAGCTCTTGCCGCAAGACC+TGG 0.482569 2:-67129593 MsaT009962.1:CDS
AGTTACGGTATGAGTAATGC+AGG 0.483173 2:-67130574 MsaT009962.1:CDS
CTAGGTGCTTATATGGTAGA+AGG 0.487203 2:-67129851 MsaT009962.1:CDS
GCACCCCACGTGCGGATCAC+AGG 0.488784 2:-67129566 MsaT009962.1:CDS
GTCGAGCGACACGAACTACT+TGG 0.488828 2:-67129062 MsaT009962.1:CDS
TATACACTAGTTGAGAAAGA+TGG 0.493554 2:-67128936 MsaT009962.1:CDS
CTCAAAATATGCCCGTGGCA+AGG 0.495295 2:-67129528 MsaT009962.1:CDS
TCAAAATATGCCCGTGGCAA+GGG 0.497303 2:-67129527 MsaT009962.1:CDS
TTTGAATATATTGTGACCCT+TGG 0.497550 2:+67130107 None:intergenic
TCGCAGCAACTTTACAGTTA+CGG 0.498690 2:-67130589 MsaT009962.1:CDS
GAAGTCTCTTTCTCCTAAAG+TGG 0.503964 2:-67129285 MsaT009962.1:CDS
GCATCCCGGTTGAGTTTCAT+GGG 0.506071 2:-67129457 MsaT009962.1:CDS
TGCTCCATGATCTATATCTC+GGG 0.508139 2:+67130137 None:intergenic
TGTCATTGCATGCGAAGGTA+AGG 0.508199 2:-67129090 MsaT009962.1:CDS
GGAGCAGCATTGTTTGGCTC+GGG 0.508648 2:-67129123 MsaT009962.1:CDS
TGGAGCAGCATTGTTTGGCT+CGG 0.508972 2:-67129124 MsaT009962.1:CDS
CGCATGTTACCAGCAGTAGC+AGG 0.509183 2:-67130075 MsaT009962.1:CDS
ATACACTAGTTGAGAAAGAT+GGG 0.509202 2:-67128935 MsaT009962.1:CDS
GTCTAAGAAATTCGGCATCC+CGG 0.511547 2:-67129471 MsaT009962.1:CDS
ATTCCTGCATGCTTCGGCAA+TGG 0.514666 2:+67129679 None:intergenic
TCTCCTTGCTATTTCTTGAG+AGG 0.515388 2:+67129156 None:intergenic
GAAGAGGATTCATCGCTACA+AGG 0.520852 2:-67130010 MsaT009962.1:CDS
TGTGGTAACTTCGTTATCAT+CGG 0.521741 2:+67130204 None:intergenic
TGTTTGATTCTTGCTCCACA+AGG 0.522993 2:+67129258 None:intergenic
TCGCTACAAGGGTTTCCATC+GGG 0.523010 2:-67129998 MsaT009962.1:CDS
GATGGAAAAGTTGACATGTA+AGG 0.524661 2:+67130547 None:intergenic
AACTCAGGAGAAATTCTCTC+TGG 0.530415 2:-67130403 MsaT009962.1:CDS
GCTCTTGCCGCAAGACCTGG+TGG 0.533032 2:-67129590 MsaT009962.1:CDS
GGCAAGGGACTCGAAGTAGT+TGG 0.533885 2:-67129512 MsaT009962.1:CDS
CCGGCCCGAGATATAGATCA+TGG 0.534861 2:-67130141 MsaT009962.1:CDS
TCTTGCCGCAAGACCTGGTG+GGG 0.535298 2:-67129588 MsaT009962.1:CDS
TTGTTTGGATCCTTGAAAGT+TGG 0.536165 2:-67130511 MsaT009962.1:CDS
AGCATCAAGGAAACAGCATC+CGG 0.536179 2:-67130160 MsaT009962.1:CDS
ATTTGATATCTGCTTCAGCT+TGG 0.537926 2:-67128884 MsaT009962.1:CDS
CGATTCCTGTGATCCGCACG+TGG 0.541378 2:+67129561 None:intergenic
GAATCAAAATGAGAGGAAAA+CGG 0.543811 2:+67130466 None:intergenic
TTTGGTTACATGGCTGCCAA+TGG 0.544829 2:-67129704 MsaT009962.1:CDS
AGAAGGTCTTGTTGCAAGGA+CGG 0.548822 2:-67129834 MsaT009962.1:CDS
AGATTGCTCAAGGAACTCAA+TGG 0.549437 2:-67129628 MsaT009962.1:CDS
ATAGACTTTCAGATTGCTCA+AGG 0.550384 2:-67129638 MsaT009962.1:CDS
AATCTCAAAAGTCCATCCCT+TGG 0.551200 2:+67129311 None:intergenic
ATCGCTACAAGGGTTTCCAT+CGG 0.551375 2:-67129999 MsaT009962.1:CDS
TCAAGTGATGAGTTATAAGA+GGG 0.555749 2:+67130373 None:intergenic
GAGCCATTGCCGAAGCATGC+AGG 0.556222 2:-67129682 MsaT009962.1:CDS
TAAGCACCTAGCCTCTGGAT+TGG 0.558693 2:+67129863 None:intergenic
AGAAACTTTAGATTACTACT+TGG 0.559206 2:-67129204 MsaT009962.1:CDS
CAGACACGGAGAAGCTTTGG+CGG 0.563257 2:-67129390 MsaT009962.1:CDS
GGGTGCCCCACCAGGTCTTG+CGG 0.563707 2:+67129583 None:intergenic
CTCTTGCCGCAAGACCTGGT+GGG 0.564705 2:-67129589 MsaT009962.1:CDS
AAGCTGTGCATGTAGAGAAA+CGG 0.566125 2:-67130042 MsaT009962.1:CDS
TTCATTGGGTGAGAGCATCA+AGG 0.568298 2:-67130173 MsaT009962.1:CDS
TACTTGGTAAGTGGAAGTCT+AGG 0.570428 2:-67129046 MsaT009962.1:CDS
TGCCGAAGCATGCAGGAATG+AGG 0.572488 2:-67129675 MsaT009962.1:CDS
GGAACTAGAAACTGCTCTGA+TGG 0.573852 2:-67130230 MsaT009962.1:CDS
GTCAATGTCATTGCATGCGA+AGG 0.575792 2:-67129095 MsaT009962.1:CDS
CTCCTGAGTTGTGTTGCTCG+TGG 0.576212 2:+67130416 None:intergenic
GAAACGGCGGAAGTTGGAAG+AGG 0.580531 2:-67130026 MsaT009962.1:CDS
TACTTCGAGTCCCTTGCCAC+GGG 0.580965 2:+67129517 None:intergenic
GACACGAACTACTTGGTAAG+TGG 0.581297 2:-67129055 MsaT009962.1:CDS
CCATGATCTATATCTCGGGC+CGG 0.582570 2:+67130141 None:intergenic
TCTTGAGAGGGTGACATCGA+TGG 0.582838 2:+67129169 None:intergenic
TGATGTTTGCGTATAATGGT+TGG 0.588941 2:+67130348 None:intergenic
TGGTAGAAGGTCTTGTTGCA+AGG 0.589192 2:-67129838 MsaT009962.1:CDS
TGCATGCGAAGGTAAGGAAA+GGG 0.591346 2:-67129084 MsaT009962.1:CDS
CTACTTCGAGTCCCTTGCCA+CGG 0.591823 2:+67129516 None:intergenic
GGGGAACCAATCCAGAGGCT+AGG 0.596146 2:-67129869 MsaT009962.1:CDS
CACCCTCTCAAGAAATAGCA+AGG 0.600143 2:-67129159 MsaT009962.1:CDS
TTGCATGCGAAGGTAAGGAA+AGG 0.601660 2:-67129085 MsaT009962.1:CDS
CATGGAGCAATGAGCACCAA+GGG 0.603617 2:-67130123 MsaT009962.1:CDS
CTAGTTCCAACAAGGCGTGT+CGG 0.604341 2:+67130245 None:intergenic
GTGTTGATGTGAATAATCCA+AGG 0.605633 2:-67129328 MsaT009962.1:CDS
AGCCACGAGCAACACAACTC+AGG 0.608623 2:-67130418 MsaT009962.1:CDS
AGTATCAGAATCAAAATGAG+AGG 0.612504 2:+67130459 None:intergenic
CTGGTGGGGCACCCCACGTG+CGG 0.613236 2:-67129574 MsaT009962.1:CDS
GATTCCTGTGATCCGCACGT+GGG 0.614125 2:+67129562 None:intergenic
ATTCCTGTGATCCGCACGTG+GGG 0.622362 2:+67129563 None:intergenic
GCTCTGAATAGCATCGCAGA+AGG 0.622655 2:+67128961 None:intergenic
TAGCAAGGAGAGGATTAATG+TGG 0.632293 2:-67129144 MsaT009962.1:CDS
GTTTGATTCTTGCTCCACAA+GGG 0.635439 2:+67129259 None:intergenic
TTGCAAGGACGGAAGCATCA+GGG 0.638897 2:-67129823 MsaT009962.1:CDS
AATTGCTTCAAATCGCCCGA+TGG 0.642596 2:+67129983 None:intergenic
ATGGGGCAATGCTCTTAGGG+TGG 0.644351 2:-67128917 MsaT009962.1:CDS
ACCCAATGAAGAATTAGATG+TGG 0.646339 2:+67130186 None:intergenic
GATTGCATGTGCCAAAGCCA+TGG 0.652232 2:-67129957 MsaT009962.1:CDS
AGCAGTTTCTAGTTCCAACA+AGG 0.652329 2:+67130237 None:intergenic
CTTAAGTGCAGAGAGCCTGA+AGG 0.653965 2:-67129782 MsaT009962.1:CDS
CTACTGCTGGTAACATGCGA+CGG 0.655149 2:+67130079 None:intergenic
TGGTAACTTCGTTATCATCG+GGG 0.655578 2:+67130206 None:intergenic
GATGTGAATAATCCAAGGGA+TGG 0.658106 2:-67129323 MsaT009962.1:CDS
GTGGAAGTCTAGGTTGACAA+TGG 0.658484 2:-67129036 MsaT009962.1:CDS
AAGAGGATTCATCGCTACAA+GGG 0.662613 2:-67130009 MsaT009962.1:CDS
GCTGTGTGATGAAGTTGCAG+TGG 0.663973 2:+67129359 None:intergenic
GAATTCACATAAGAACTCAA+AGG 0.664196 2:+67128996 None:intergenic
TCATGGAGCAATGAGCACCA+AGG 0.677430 2:-67130124 MsaT009962.1:CDS
TCTCAAGAAATAGCAAGGAG+AGG 0.677996 2:-67129154 MsaT009962.1:CDS
CTGTGCATGTAGAGAAACGG+CGG 0.685656 2:-67130039 MsaT009962.1:CDS
GACATGCTTGATATCAGACA+CGG 0.687158 2:-67129404 MsaT009962.1:CDS
CCAGTCTCAAAATATGCCCG+TGG 0.687941 2:-67129533 MsaT009962.1:CDS
GTTACGGTATGAGTAATGCA+GGG 0.691425 2:-67130573 MsaT009962.1:CDS
GGTAACTTCGTTATCATCGG+GGG 0.691927 2:+67130207 None:intergenic
CAAGTGATGAGTTATAAGAG+GGG 0.693938 2:+67130374 None:intergenic
TGTTGATGTGAATAATCCAA+GGG 0.710085 2:-67129327 MsaT009962.1:CDS
AAATGCTGTGTCTATCAATG+GGG 0.714938 2:-67129888 MsaT009962.1:CDS
TACACTAGTTGAGAAAGATG+GGG 0.719725 2:-67128934 MsaT009962.1:CDS
GTATCCCATGAAACTCAACC+GGG 0.730080 2:+67129453 None:intergenic
TCAATGGGGAACCAATCCAG+AGG 0.733104 2:-67129874 MsaT009962.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 67128878 67130617 67128878 ID=MsaG009962
Chr2 mRNA 67128878 67130617 67128878 ID=MsaT009962.1;Parent=MsaG009962
Chr2 exon 67128878 67130617 67128878 ID=MsaT009962.1.exon1;Parent=MsaT009962.1
Chr2 CDS 67128878 67130617 67128878 ID=cds.MsaT009962.1;Parent=MsaT009962.1
Gene Sequence

>MsaT009962.1

ATGGACTCGCAGCAACTTTACAGTTACGGTATGAGTAATGCAGGGTTACCTTACATGTCAACTTTTCCATCACTGCCAAATGGTTTGTTTGGATCCTTGAAAGTTGGCTTTGGAAACTCACCCGATTCACCGTTTTCCTCTCATTTTGATTCTGATACTCTTTCTGCATTTAGCGATAGCCACGAGCAACACAACTCAGGAGAAATTCTCTCTGGTATAAGCCCCTCTTATAACTCATCACTTGAAACCAACCATTATACGCAAACATCATTCTCTTCGATCAACAGTCTCAAAGACAGTTTGCAACTATATTCTGCTAGAAATTCTTTTCTACCAAGTAACCAGAAAATCCGACACGCCTTGTTGGAACTAGAAACTGCTCTGATGGCCCCCGATGATAACGAAGTTACCACATCTAATTCTTCATTGGGTGAGAGCATCAAGGAAACAGCATCCGGCCCGAGATATAGATCATGGAGCAATGAGCACCAAGGGTCACAATATATTCAAAGTCAACCGTCGCATGTTACCAGCAGTAGCAGGCAATCAAATGAAGCTGTGCATGTAGAGAAACGGCGGAAGTTGGAAGAGGATTCATCGCTACAAGGGTTTCCATCGGGCGATTTGAAGCAATTACTGATTGCATGTGCCAAAGCCATGGCTGAAAACAACACAGAAGATTTTGACCGATTGATAGAAACGGCTAGAAATGCTGTGTCTATCAATGGGGAACCAATCCAGAGGCTAGGTGCTTATATGGTAGAAGGTCTTGTTGCAAGGACGGAAGCATCAGGGAATAGTATCTATCATGCCCTTAAGTGCAGAGAGCCTGAAGGTGAAGAATTACTCACTTACATGCAACTGCTTTTCGAAATCTGTCCCTACTTAAAATTTGGTTACATGGCTGCCAATGGAGCCATTGCCGAAGCATGCAGGAATGAGGATCACATACACATCATAGACTTTCAGATTGCTCAAGGAACTCAATGGATGACACTTCTTCAAGCTCTTGCCGCAAGACCTGGTGGGGCACCCCACGTGCGGATCACAGGAATCGACGATCCAGTCTCAAAATATGCCCGTGGCAAGGGACTCGAAGTAGTTGGAGAGAGATTAGCTTTGATGTCTAAGAAATTCGGCATCCCGGTTGAGTTTCATGGGATACCTGTTTTCGGTCCAGATGTGACAAGAGACATGCTTGATATCAGACACGGAGAAGCTTTGGCGGTGAATTTTCCACTGCAACTTCATCACACAGCTGATGAGAGTGTTGATGTGAATAATCCAAGGGATGGACTTTTGAGATTGGTGAAGTCTCTTTCTCCTAAAGTGGTTACCCTTGTGGAGCAAGAATCAAACACAAACACAACACCTTTCTTCAACAGGTTCATAGAAACTTTAGATTACTACTTGGCAATCTTCGAGTCCATCGATGTCACCCTCTCAAGAAATAGCAAGGAGAGGATTAATGTGGAGCAGCATTGTTTGGCTCGGGATATCGTCAATGTCATTGCATGCGAAGGTAAGGAAAGGGTCGAGCGACACGAACTACTTGGTAAGTGGAAGTCTAGGTTGACAATGGCTGGATTTCGTCAATGTCCTTTGAGTTCTTATGTGAATTCTGTTATAAGAAGCCTTCTGCGATGCTATTCAGAGCACTATACACTAGTTGAGAAAGATGGGGCAATGCTCTTAGGGTGGAAGAACAGAAATTTGATATCTGCTTCAGCTTGGCATTGA
Protein sequence

>MsaT009962.1

MDSQQLYSYGMSNAGLPYMSTFPSLPNGLFGSLKVGFGNSPDSPFSSHFDSDTLSAFSDSHEQHNSGEILSGISPSYNSSLETNHYTQTSFSSINSLKDSLQLYSARNSFLPSNQKIRHALLELETALMAPDDNEVTTSNSSLGESIKETASGPRYRSWSNEHQGSQYIQSQPSHVTSSSRQSNEAVHVEKRRKLEEDSSLQGFPSGDLKQLLIACAKAMAENNTEDFDRLIETARNAVSINGEPIQRLGAYMVEGLVARTEASGNSIYHALKCREPEGEELLTYMQLLFEICPYLKFGYMAANGAIAEACRNEDHIHIIDFQIAQGTQWMTLLQALAARPGGAPHVRITGIDDPVSKYARGKGLEVVGERLALMSKKFGIPVEFHGIPVFGPDVTRDMLDIRHGEALAVNFPLQLHHTADESVDVNNPRDGLLRLVKSLSPKVVTLVEQESNTNTTPFFNRFIETLDYYLAIFESIDVTLSRNSKERINVEQHCLARDIVNVIACEGKERVERHELLGKWKSRLTMAGFRQCPLSSYVNSVIRSLLRCYSEHYTLVEKDGAMLLGWKNRNLISASAWH*