AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010039

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010039 MtrunA17_Chr2g0319961 95.402 261 12 0 1 261 1 261 4.81e-170 469
MsaG010039 MtrunA17_Chr8g0379461 53.171 205 66 7 50 247 45 226 2.53e-60 191
MsaG010039 MtrunA17_Chr2g0284321 52.239 201 73 7 48 245 8 188 4.08e-57 181
MsaG010039 MtrunA17_Chr2g0293481 61.386 101 38 1 50 150 238 337 2.38e-35 131
MsaG010039 MtrunA17_Chr7g0248691 54.206 107 38 1 44 150 129 224 2.56e-32 120
MsaG010039 MtrunA17_Chr7g0270051 50.000 132 48 5 50 180 25 139 1.34e-31 117
MsaG010039 MtrunA17_Chr5g0401821 51.961 102 33 2 50 146 175 265 1.49e-28 109
MsaG010039 MtrunA17_Chr8g0348311 50.962 104 40 1 47 150 133 225 6.01e-28 109
MsaG010039 MtrunA17_Chr8g0387501 61.905 84 31 1 81 163 130 213 3.14e-27 106
MsaG010039 MtrunA17_Chr4g0053281 58.242 91 31 3 50 139 241 325 4.51e-27 107
MsaG010039 MtrunA17_Chr4g0071521 54.255 94 27 2 50 138 221 303 9.28e-27 105
MsaG010039 MtrunA17_Chr1g0147881 55.556 90 34 2 50 139 238 321 9.64e-27 106
MsaG010039 MtrunA17_Chr1g0202461 44.915 118 55 1 40 157 231 338 4.49e-26 105
MsaG010039 MtrunA17_Chr1g0184691 52.174 92 28 2 52 138 271 351 2.17e-24 100
MsaG010039 MtrunA17_Chr7g0228941 63.333 60 22 0 79 138 51 110 4.55e-24 94.0
MsaG010039 MtrunA17_Chr3g0139021 42.500 120 56 2 21 138 264 372 1.27e-23 98.6
MsaG010039 MtrunA17_Chr2g0289181 59.677 62 25 0 79 140 13 74 7.45e-22 90.9
MsaG010039 MtrunA17_Chr7g0270831 42.276 123 56 3 38 160 183 290 5.24e-20 88.6
MsaG010039 MtrunA17_Chr3g0099641 46.512 86 46 0 71 156 242 327 1.12e-19 87.8
MsaG010039 MtrunA17_Chr4g0025061 42.553 94 54 0 71 164 351 444 2.97e-19 87.0
MsaG010039 MtrunA17_Chr2g0288871 54.286 70 32 0 71 140 296 365 1.11e-18 85.1
MsaG010039 MtrunA17_Chr4g0051881 45.745 94 47 3 81 174 179 268 1.24e-18 84.0
MsaG010039 MtrunA17_Chr4g0025051 41.489 94 55 0 71 164 326 419 1.68e-18 84.7
MsaG010039 MtrunA17_Chr2g0322391 51.429 70 34 0 71 140 340 409 5.19e-18 83.2
MsaG010039 MtrunA17_Chr7g0218081 35.119 168 85 6 13 160 327 490 5.61e-18 83.2
MsaG010039 MtrunA17_Chr7g0218081 40.217 92 50 3 78 166 226 315 8.02e-12 64.7
MsaG010039 MtrunA17_Chr4g0051181 52.113 71 34 0 71 141 234 304 5.93e-18 82.8
MsaG010039 MtrunA17_Chr4g0075201 37.008 127 62 3 18 140 215 327 7.53e-18 82.8
MsaG010039 MtrunA17_Chr4g0060271 52.113 71 34 0 71 141 246 316 1.49e-17 82.0
MsaG010039 MtrunA17_Chr4g0042831 38.095 105 55 2 41 145 248 342 2.32e-17 80.9
MsaG010039 MtrunA17_Chr4g0042831 35.632 87 55 1 81 167 146 231 2.13e-11 63.2
MsaG010039 MtrunA17_Chr7g0263531 57.377 61 25 1 81 141 362 421 2.35e-17 80.9
MsaG010039 MtrunA17_Chr7g0263531 45.946 74 38 2 78 151 187 258 1.24e-13 70.1
MsaG010039 MtrunA17_Chr5g0418191 42.857 84 47 1 81 164 321 403 2.40e-17 80.9
MsaG010039 MtrunA17_Chr5g0418191 48.485 66 32 2 78 143 149 212 1.15e-13 70.1
MsaG010039 MtrunA17_Chr4g0074001 38.889 108 60 3 44 146 318 424 4.33e-17 80.5
MsaG010039 MtrunA17_Chr5g0443131 56.667 60 25 1 81 140 158 216 5.68e-17 79.0
MsaG010039 MtrunA17_Chr5g0418781 39.024 123 65 4 37 154 85 202 1.01e-16 77.8
MsaG010039 MtrunA17_Chr5g0445311 50.000 64 31 1 81 144 365 427 1.06e-16 79.3
MsaG010039 MtrunA17_Chr7g0216891 44.048 84 47 0 71 154 210 293 1.70e-16 79.0
MsaG010039 MtrunA17_Chr2g0302391 55.000 60 27 0 81 140 212 271 1.87e-16 78.2
MsaG010039 MtrunA17_Chr3g0101131 35.374 147 85 7 12 151 341 484 2.54e-16 78.2
MsaG010039 MtrunA17_Chr4g0024391 38.835 103 58 2 71 170 220 320 2.90e-16 77.8
MsaG010039 MtrunA17_Chr7g0249311 34.848 132 76 4 81 207 393 519 3.88e-16 77.8
MsaG010039 MtrunA17_Chr7g0249311 50.000 60 28 2 81 140 180 237 1.86e-11 63.9
MsaG010039 MtrunA17_Chr3g0136961 42.045 88 46 1 81 163 139 226 7.32e-16 75.5
MsaG010039 MtrunA17_Chr3g0079691 40.698 86 46 2 81 166 496 576 2.07e-15 75.9
MsaG010039 MtrunA17_Chr3g0079691 43.333 90 48 3 78 167 278 364 6.71e-14 71.2
MsaG010039 MtrunA17_Chr1g0150281 52.632 57 27 0 81 137 141 197 2.19e-15 74.3
MsaG010039 MtrunA17_Chr7g0270191 35.965 114 55 3 81 194 146 241 2.87e-15 73.9
MsaG010039 MtrunA17_Chr6g0485431 52.381 63 28 2 80 141 154 215 3.53e-15 74.3
MsaG010039 MtrunA17_Chr8g0383251 42.683 82 45 2 71 152 99 178 4.21e-15 71.6
MsaG010039 MtrunA17_Chr7g0238921 57.895 57 23 1 81 137 125 180 5.76e-15 73.2
MsaG010039 MtrunA17_Chr1g0194481 50.877 57 27 1 81 137 159 214 8.85e-15 72.8
MsaG010039 MtrunA17_Chr3g0106491 49.206 63 31 1 81 143 366 427 9.53e-15 73.6
MsaG010039 MtrunA17_Chr3g0129321 31.967 122 74 2 51 172 89 201 1.32e-14 70.5
MsaG010039 MtrunA17_Chr4g0004911 49.206 63 31 1 81 143 337 398 1.51e-14 72.8
MsaG010039 MtrunA17_Chr7g0249301 49.206 63 31 1 81 143 293 354 1.60e-14 72.4
MsaG010039 MtrunA17_Chr7g0249301 51.667 60 27 2 81 140 63 120 1.15e-12 67.0
MsaG010039 MtrunA17_Chr5g0447701 50.820 61 29 1 80 140 252 311 2.89e-14 71.6
MsaG010039 MtrunA17_Chr4g0068541 42.254 71 40 1 71 141 199 268 4.02e-14 71.6
MsaG010039 MtrunA17_Chr7g0275531 45.455 88 42 3 50 135 92 175 5.13e-14 70.9
MsaG010039 MtrunA17_Chr5g0432161 36.154 130 57 5 17 143 377 483 6.25e-14 71.2
MsaG010039 MtrunA17_Chr3g0126391 38.532 109 53 3 33 135 90 190 6.62e-14 70.5
MsaG010039 MtrunA17_Chr1g0188941 45.161 62 33 1 81 142 52 112 8.00e-14 66.2
MsaG010039 MtrunA17_Chr4g0001361 48.333 60 31 0 81 140 163 222 9.33e-14 69.7
MsaG010039 MtrunA17_Chr1g0150271 43.939 66 37 0 75 140 96 161 1.35e-13 68.6
MsaG010039 MtrunA17_Chr1g0150801 37.736 106 54 3 34 137 63 158 1.39e-13 67.0
MsaG010039 MtrunA17_Chr2g0294231 48.333 60 30 1 81 140 204 262 4.31e-13 68.2
MsaG010039 MtrunA17_Chr2g0333851 47.368 57 30 0 81 137 170 226 5.22e-13 67.8
MsaG010039 MtrunA17_Chr7g0243891 46.032 63 33 1 81 143 375 436 8.33e-13 67.8
MsaG010039 MtrunA17_Chr7g0224801 32.000 125 79 3 23 144 33 154 1.53e-12 64.3
MsaG010039 MtrunA17_Chr1g0189391 54.237 59 25 2 81 138 98 155 2.71e-12 65.9
MsaG010039 MtrunA17_Chr8g0387981 33.613 119 69 2 27 135 71 189 2.71e-12 65.9
MsaG010039 MtrunA17_Chr8g0347251 35.514 107 54 4 46 140 100 203 5.32e-12 65.1
MsaG010039 MtrunA17_Chr5g0431811 37.168 113 58 5 40 142 629 738 8.45e-12 65.1
MsaG010039 MtrunA17_Chr8g0335811 39.286 84 49 2 71 154 145 226 1.10e-11 62.8
MsaG010039 MtrunA17_Chr8g0383351 46.552 58 30 1 81 138 161 217 1.46e-11 62.8
MsaG010039 MtrunA17_Chr3g0129361 46.552 58 30 1 81 138 145 201 2.80e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010039 AT3G58710.2 50.781 256 86 7 26 256 16 256 7.50e-74 226
MsaG010039 AT3G58710.1 50.584 257 86 8 26 256 16 257 5.14e-72 222
MsaG010039 AT1G29280.1 47.303 241 104 6 26 249 15 249 1.48e-61 195
MsaG010039 AT2G34830.2 47.368 171 69 5 37 188 178 346 4.82e-37 135
MsaG010039 AT2G34830.1 47.368 171 69 5 37 188 169 337 4.85e-37 135
MsaG010039 AT1G30650.1 64.211 95 33 1 50 144 188 281 1.67e-35 131
MsaG010039 AT4G01250.1 45.926 135 58 2 21 150 74 198 3.39e-29 112
MsaG010039 AT5G45050.2 42.759 145 63 3 43 175 1123 1259 5.16e-29 115
MsaG010039 AT5G45050.1 42.759 145 63 3 43 175 1151 1287 5.91e-29 115
MsaG010039 AT5G45050.4 42.759 145 63 3 43 175 1012 1148 7.10e-29 115
MsaG010039 AT5G52830.1 49.600 125 47 3 80 191 165 286 7.89e-28 109
MsaG010039 AT4G23550.1 66.197 71 24 0 80 150 140 210 8.83e-28 108
MsaG010039 AT4G24240.1 53.608 97 29 2 50 141 257 342 4.87e-27 107
MsaG010039 AT2G23320.1 54.444 90 35 2 50 139 216 299 1.05e-26 105
MsaG010039 AT2G30590.1 53.763 93 27 2 50 137 289 370 8.28e-26 104
MsaG010039 AT4G31550.2 53.191 94 28 2 50 138 221 303 1.66e-25 102
MsaG010039 AT4G31550.1 52.128 94 29 2 50 138 222 304 1.00e-24 100
MsaG010039 AT5G28650.1 52.577 97 30 2 50 141 238 323 2.20e-24 100
MsaG010039 AT3G04670.1 52.577 97 30 2 50 141 238 323 3.02e-24 99.8
MsaG010039 AT2G24570.1 49.505 101 35 2 50 145 219 308 3.18e-24 99.4
MsaG010039 AT5G45260.1 42.520 127 53 5 37 155 1171 1285 8.09e-22 95.1
MsaG010039 AT4G30935.1 38.596 114 62 3 39 146 285 396 1.07e-20 91.3
MsaG010039 AT4G04450.2 46.809 94 50 0 71 164 189 282 4.98e-20 89.0
MsaG010039 AT4G04450.1 46.809 94 50 0 71 164 283 376 7.16e-20 89.0
MsaG010039 AT5G15130.1 48.810 84 43 0 71 154 218 301 2.66e-19 87.4
MsaG010039 AT5G15130.2 48.810 84 43 0 71 154 176 259 2.89e-19 87.0
MsaG010039 AT1G68150.1 46.988 83 44 0 71 153 226 308 7.71e-19 85.1
MsaG010039 AT4G22070.4 52.857 70 33 0 71 140 288 357 1.85e-18 85.1
MsaG010039 AT4G22070.3 52.857 70 33 0 71 140 288 357 1.85e-18 85.1
MsaG010039 AT4G22070.2 52.857 70 33 0 71 140 288 357 1.85e-18 85.1
MsaG010039 AT4G22070.1 52.857 70 33 0 71 140 288 357 1.85e-18 85.1
MsaG010039 AT1G62300.1 36.957 138 67 3 3 140 255 372 2.32e-18 84.7
MsaG010039 AT2G04880.2 59.016 61 24 1 80 140 283 342 5.39e-18 83.6
MsaG010039 AT2G04880.1 59.016 61 24 1 80 140 307 366 5.39e-18 83.6
MsaG010039 AT5G46350.1 47.619 84 38 2 81 164 184 261 6.51e-18 82.0
MsaG010039 AT5G26170.1 51.471 68 32 1 71 138 104 170 7.36e-18 79.0
MsaG010039 AT2G37260.3 40.952 105 56 2 37 141 231 329 8.60e-18 82.0
MsaG010039 AT2G37260.3 48.649 74 33 3 81 151 86 157 2.87e-12 66.2
MsaG010039 AT2G37260.1 40.952 105 56 2 37 141 311 409 9.97e-18 82.4
MsaG010039 AT2G37260.1 48.649 74 33 3 81 151 166 237 2.05e-12 67.0
MsaG010039 AT2G37260.2 40.952 105 56 2 37 141 229 327 1.03e-17 81.6
MsaG010039 AT2G37260.2 48.649 74 33 3 81 151 84 155 3.22e-12 65.9
MsaG010039 AT1G13960.2 38.281 128 59 4 13 140 208 315 3.32e-17 80.5
MsaG010039 AT1G13960.2 49.206 63 30 2 78 140 74 134 5.24e-13 68.6
MsaG010039 AT1G13960.1 38.281 128 59 4 13 140 361 468 3.48e-17 81.3
MsaG010039 AT1G13960.1 49.206 63 30 2 78 140 227 287 6.82e-13 68.6
MsaG010039 AT4G01720.1 51.429 70 34 0 71 140 230 299 3.71e-17 80.9
MsaG010039 AT4G18170.1 56.667 60 25 1 81 140 173 231 4.79e-17 79.7
MsaG010039 AT3G62340.1 42.222 90 45 2 52 141 96 178 5.69e-17 79.0
MsaG010039 AT1G69810.1 47.368 76 39 1 80 154 203 278 1.22e-16 79.0
MsaG010039 AT1G69810.2 47.368 76 39 1 80 154 145 220 1.56e-16 78.2
MsaG010039 AT1G18860.1 52.857 70 33 0 71 140 230 299 1.58e-16 79.3
MsaG010039 AT1G29860.1 55.000 60 26 1 81 140 137 195 1.79e-16 77.4
MsaG010039 AT5G49520.1 51.515 66 30 2 81 145 222 286 2.55e-16 78.2
MsaG010039 AT4G26640.5 32.759 116 67 2 81 186 206 320 3.64e-16 77.8
MsaG010039 AT4G26640.6 32.759 116 67 2 81 186 206 320 3.64e-16 77.8
MsaG010039 AT4G26640.4 32.759 116 67 2 81 186 206 320 3.64e-16 77.8
MsaG010039 AT4G26640.3 32.759 116 67 2 81 186 206 320 3.64e-16 77.8
MsaG010039 AT4G26640.1 32.759 116 67 2 81 186 310 424 7.00e-16 77.4
MsaG010039 AT4G26640.2 32.759 116 67 2 81 186 382 496 9.02e-16 77.0
MsaG010039 AT4G26640.7 32.759 116 67 2 81 186 342 456 1.04e-15 76.6
MsaG010039 AT2G03340.1 34.228 149 79 6 11 144 334 478 1.05e-15 76.6
MsaG010039 AT2G03340.1 43.056 72 39 2 78 149 248 317 3.52e-13 69.3
MsaG010039 AT5G07100.2 49.206 63 31 1 81 143 142 203 1.28e-15 73.9
MsaG010039 AT5G07100.2 30.435 138 88 2 62 192 6 142 7.62e-12 63.5
MsaG010039 AT5G07100.4 49.206 63 31 1 81 143 142 203 1.28e-15 73.9
MsaG010039 AT5G07100.4 30.435 138 88 2 62 192 6 142 7.62e-12 63.5
MsaG010039 AT5G07100.5 49.206 63 31 1 81 143 142 203 1.28e-15 73.9
MsaG010039 AT5G07100.5 30.435 138 88 2 62 192 6 142 7.62e-12 63.5
MsaG010039 AT5G07100.3 49.206 63 31 1 81 143 142 203 1.28e-15 73.9
MsaG010039 AT5G07100.3 30.435 138 88 2 62 192 6 142 7.62e-12 63.5
MsaG010039 AT4G31800.2 42.857 77 44 0 81 157 112 188 2.55e-15 73.9
MsaG010039 AT5G07100.1 49.206 63 31 1 81 143 235 296 2.69e-15 74.7
MsaG010039 AT5G07100.1 30.435 138 88 2 62 192 99 235 3.57e-11 62.8
MsaG010039 AT2G40740.1 37.719 114 63 3 50 158 146 256 2.95e-15 74.3
MsaG010039 AT2G40740.3 37.719 114 63 3 50 158 155 265 3.20e-15 74.3
MsaG010039 AT2G47260.1 35.354 99 54 2 43 141 146 234 3.74e-15 74.3
MsaG010039 AT4G31800.1 42.857 77 44 0 81 157 177 253 4.84e-15 73.9
MsaG010039 AT4G11070.2 36.429 140 77 5 16 143 37 176 7.92e-15 72.8
MsaG010039 AT2G38470.1 49.206 63 31 1 81 143 363 424 8.73e-15 73.9
MsaG010039 AT4G11070.1 32.418 182 107 7 16 181 69 250 9.25e-15 73.2
MsaG010039 AT1G69310.4 37.113 97 51 2 41 137 117 203 1.09e-14 72.8
MsaG010039 AT1G69310.2 37.113 97 51 2 41 137 117 203 1.09e-14 72.8
MsaG010039 AT1G69310.1 37.113 97 51 2 41 137 117 203 1.09e-14 72.8
MsaG010039 AT2G25000.2 50.000 60 30 0 81 140 104 163 1.47e-14 71.2
MsaG010039 AT2G25000.4 50.000 60 30 0 81 140 104 163 1.47e-14 71.2
MsaG010039 AT2G21900.1 38.261 115 63 3 27 140 61 168 1.55e-14 70.9
MsaG010039 AT2G25000.3 50.000 60 30 0 81 140 126 185 2.12e-14 71.2
MsaG010039 AT2G30250.1 43.373 83 37 2 58 140 315 387 2.78e-14 72.4
MsaG010039 AT2G25000.1 50.000 60 30 0 81 140 147 206 3.20e-14 71.2
MsaG010039 AT3G01080.2 39.796 98 51 3 47 140 310 403 3.43e-14 72.4
MsaG010039 AT3G01080.2 49.206 63 30 2 78 140 203 263 2.52e-12 66.6
MsaG010039 AT3G01080.1 39.796 98 51 3 47 140 272 365 4.02e-14 72.0
MsaG010039 AT3G01080.1 49.206 63 30 2 78 140 165 225 2.91e-12 66.2
MsaG010039 AT5G56270.1 47.619 63 32 1 81 143 488 549 4.74e-14 72.0
MsaG010039 AT5G56270.1 46.970 66 33 2 78 143 271 334 1.46e-11 64.7
MsaG010039 AT4G26440.1 32.479 117 69 2 46 162 347 453 1.67e-13 70.5
MsaG010039 AT4G26440.1 48.485 66 32 2 78 143 176 239 6.59e-12 65.5
MsaG010039 AT1G80840.1 45.000 60 33 0 81 140 147 206 1.78e-13 69.3
MsaG010039 AT5G13080.1 41.892 74 40 2 71 144 58 128 5.75e-13 65.1
MsaG010039 AT4G23810.1 41.237 97 50 2 50 144 133 224 9.08e-13 67.4
MsaG010039 AT3G01970.1 38.272 81 47 2 71 149 56 135 1.08e-12 64.3
MsaG010039 AT3G01080.3 49.206 63 30 2 78 140 203 263 1.98e-12 66.6
MsaG010039 AT2G46400.1 31.579 152 87 5 81 221 105 250 2.15e-12 66.2
MsaG010039 AT5G24110.1 30.201 149 81 4 24 168 63 192 3.34e-12 65.9
MsaG010039 AT3G56400.1 31.081 148 66 6 25 146 57 194 4.96e-12 65.1
MsaG010039 AT1G64000.1 47.368 57 29 1 81 137 115 170 6.10e-12 63.5
MsaG010039 AT2G46130.1 47.368 57 29 1 81 137 31 86 1.86e-11 60.1
MsaG010039 AT5G41570.1 47.368 57 29 1 81 137 99 154 2.39e-11 61.6
MsaG010039 AT1G66560.1 44.444 72 38 1 66 135 89 160 2.83e-11 62.4
MsaG010039 AT5G64810.1 45.614 57 30 1 81 137 111 166 3.54e-11 61.2
MsaG010039 AT2G44745.1 47.368 57 29 1 81 137 146 201 4.45e-11 61.6
MsaG010039 AT5G43290.1 50.877 57 27 1 81 137 115 170 4.50e-11 62.0
MsaG010039 AT4G39410.1 44.118 68 37 1 71 138 214 280 5.07e-11 62.4
MsaG010039 AT1G55600.1 43.548 62 34 1 79 140 306 366 7.07e-11 62.4
MsaG010039 AT4G12020.10 39.744 78 44 3 80 157 468 542 8.68e-11 62.4
MsaG010039 AT4G12020.3 39.744 78 44 3 80 157 468 542 8.93e-11 62.4
MsaG010039 AT4G12020.6 39.744 78 44 3 80 157 468 542 9.12e-11 62.4
MsaG010039 AT4G12020.8 39.744 78 44 3 80 157 468 542 9.12e-11 62.4
MsaG010039 AT4G12020.2 39.744 78 44 3 80 157 468 542 9.71e-11 62.4
MsaG010039 AT4G12020.4 39.744 78 44 3 80 157 468 542 9.88e-11 62.4

Find 93 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAAGAATGATGAGATTT+TGG 0.182722 2:+68091606 None:intergenic
TTTCAGTATAGCGGCATTAC+AGG 0.325170 2:-68091270 MsaT010039.1:CDS
GGCGATCACAACGCCGTGTT+TGG 0.338656 2:-68091459 MsaT010039.1:CDS
CTCGAGCTTGAGCTCAGGCT+CGG 0.342839 2:+68095978 None:intergenic
GCGAAAACGGGCCGATCTTC+CGG 0.342902 2:+68091507 None:intergenic
GGTGGCGGTGACGGCGGTGG+TGG 0.345885 2:+68091587 None:intergenic
CGGATAAGGCGAGCCTTTGA+TGG 0.367115 2:+68095542 None:intergenic
TGCCGATCTCGGAGAGTTGC+CGG 0.372131 2:-68091334 MsaT010039.1:CDS
GGAGGGAGTGGTTGTGTTCA+TGG 0.377427 2:+68091634 None:intergenic
CATATCCACCGTCGGATTCA+TGG 0.384899 2:-68095590 MsaT010039.1:CDS
GTCGGGATGAGTGGCGAAAA+CGG 0.412803 2:+68091494 None:intergenic
AGCTGTGACGGTGGCGGTGA+CGG 0.424921 2:+68091578 None:intergenic
AGCAGGCCCCGCTTCTGAGA+TGG 0.433634 2:+68095954 None:intergenic
CACCGTCGGATTCATGGGCT+TGG 0.434014 2:-68095584 MsaT010039.1:CDS
TCCTCTACTCCGCCGCATAT+CGG 0.434523 2:+68091396 None:intergenic
TAGGTTACGAGGAGGTTTGT+GGG 0.435902 2:+68091660 None:intergenic
TCCTTCTCCCGGCGAGTCTC+CGG 0.439306 2:-68091544 MsaT010039.1:CDS
GATCCTTCCACGTCAGCAAT+TGG 0.441186 2:+68095630 None:intergenic
TCGAGCTTGAGCTCAGGCTC+GGG 0.444741 2:+68095979 None:intergenic
CTCGAGCCCATCTCAGAAGC+GGG 0.454610 2:-68095961 MsaT010039.1:CDS
AAATTATAGTACCTCGGATA+AGG 0.459235 2:+68095528 None:intergenic
TCGGGATGAGTGGCGAAAAC+GGG 0.459881 2:+68091495 None:intergenic
TCACAAGAAGAGGGAGAAGC+AGG 0.462868 2:+68095937 None:intergenic
GCAGGCCCCGCTTCTGAGAT+GGG 0.464707 2:+68095955 None:intergenic
TCCGGCTGCCACTCCTCAGC+CGG 0.468481 2:-68091526 MsaT010039.1:CDS
GACGGTGGCGGTGACGGCGG+TGG 0.469462 2:+68091584 None:intergenic
GTAGGTTACGAGGAGGTTTG+TGG 0.474952 2:+68091659 None:intergenic
CCGCTATACTGAAACACACT+TGG 0.499608 2:+68091279 None:intergenic
TCCGGCTGAGGAGTGGCAGC+CGG 0.500132 2:+68091525 None:intergenic
ATATCCACCGTCGGATTCAT+GGG 0.503432 2:-68095589 MsaT010039.1:CDS
TACCGATGTAGTAGTTCAAA+AGG 0.506115 2:-68091723 MsaT010039.1:CDS
ATGGGCTCGAGCTTGAGCTC+AGG 0.506629 2:+68095973 None:intergenic
GCCGGAGACTCGCCGGGAGA+AGG 0.506970 2:+68091543 None:intergenic
AAGAACCATCACCCAAGAAA+AGG 0.507877 2:-68095900 MsaT010039.1:intron
TCCGATATGCGGCGGAGTAG+AGG 0.508135 2:-68091397 MsaT010039.1:CDS
ACGGGCCGATCTTCCGGCTG+AGG 0.512946 2:+68091513 None:intergenic
GAGGGAGTGGTTGTGTTCAT+GGG 0.513550 2:+68091635 None:intergenic
GAGCCGTTGTTTGCCGATCT+CGG 0.514966 2:-68091345 MsaT010039.1:CDS
ATCACAACGCCGTGTTTGGA+TGG 0.519613 2:-68091455 MsaT010039.1:CDS
TTTAGTGTCTTCACAAGAAG+AGG 0.522076 2:+68095927 None:intergenic
AGTGGCAGCCGGAGACTCGC+CGG 0.523423 2:+68091536 None:intergenic
TTCGATGATGTTGTATCCAC+CGG 0.526361 2:-68091432 MsaT010039.1:CDS
CCACTCCTCAGCCGGAAGAT+CGG 0.530292 2:-68091518 MsaT010039.1:CDS
GCTCAGGCTCGGGCTCATCT+CGG 0.538319 2:+68095989 None:intergenic
CACTTGGAATATTTCTCTGT+CGG 0.542054 2:+68091295 None:intergenic
GGATAAGGCGAGCCTTTGAT+GGG 0.543319 2:+68095543 None:intergenic
GTCGACGGCAGCTGTGACGG+TGG 0.545751 2:+68091569 None:intergenic
AGTGTCGACGGCAGCTGTGA+CGG 0.553848 2:+68091566 None:intergenic
GTGGCAGCCGGAGACTCGCC+GGG 0.561605 2:+68091537 None:intergenic
TACGGTCAAAAGCCCATCAA+AGG 0.561969 2:-68095555 MsaT010039.1:CDS
GTGGTTGTGTTCATGGGCGT+AGG 0.562233 2:+68091641 None:intergenic
TCATGGGCTTGGAGAAAGTA+CGG 0.563892 2:-68095573 MsaT010039.1:CDS
ATACCAATTGCTGACGTGGA+AGG 0.566426 2:-68095633 MsaT010039.1:CDS
ATAGGAGGGAGATGAAGAAG+AGG 0.573853 2:-68095665 MsaT010039.1:intron
AGAGTTGCCGGAGTGTTCAA+CGG 0.576289 2:-68091322 MsaT010039.1:CDS
CTCCGGCAACTCTCCGAGAT+CGG 0.577094 2:+68091332 None:intergenic
TCGCCGGTGAGGTCAAAGTC+GGG 0.577492 2:+68091477 None:intergenic
CCAAGTGTGTTTCAGTATAG+CGG 0.578263 2:-68091279 MsaT010039.1:CDS
CCGATCTTCCGGCTGAGGAG+TGG 0.579167 2:+68091518 None:intergenic
GAGGGAGATGAAGAAGAGGG+TGG 0.583069 2:-68095661 MsaT010039.1:CDS
CGGAAAACCGTTGAACACTC+CGG 0.588243 2:+68091315 None:intergenic
ATCGCCGGTGAGGTCAAAGT+CGG 0.588374 2:+68091476 None:intergenic
AAACACGGCGTTGTGATCGC+CGG 0.589318 2:+68091461 None:intergenic
CTCCAAGCCCATGAATCCGA+CGG 0.591645 2:+68095582 None:intergenic
GAGGTCAAAGTCGGGATGAG+TGG 0.592534 2:+68091485 None:intergenic
TTAGTGTCTTCACAAGAAGA+GGG 0.596042 2:+68095928 None:intergenic
GCCGTCGACACTCCTTCTCC+CGG 0.599041 2:-68091555 MsaT010039.1:CDS
GACTATACCAATTGCTGACG+TGG 0.603395 2:-68095637 MsaT010039.1:CDS
GCTCGAGCCCATCTCAGAAG+CGG 0.603968 2:-68095962 MsaT010039.1:CDS
GGAGGTTTGTGGGGTCTACA+CGG 0.606363 2:+68091670 None:intergenic
GTGGAAGGATCTAAGAGTAG+AGG 0.612807 2:-68095618 MsaT010039.1:CDS
GTTCATGGGCGTAGGTTACG+AGG 0.614339 2:+68091649 None:intergenic
GACGGCAGCTGTGACGGTGG+CGG 0.623123 2:+68091572 None:intergenic
TCGAGCCCATCTCAGAAGCG+GGG 0.626614 2:-68095960 MsaT010039.1:CDS
GCCGGGAGAAGGAGTGTCGA+CGG 0.631416 2:+68091554 None:intergenic
TAGGAGGGAGATGAAGAAGA+GGG 0.638622 2:-68095664 MsaT010039.1:CDS
GTCGTTGACGATGCGAGAGG+AGG 0.638646 2:-68091370 MsaT010039.1:CDS
AGGTTACGAGGAGGTTTGTG+GGG 0.641192 2:+68091661 None:intergenic
CTCGTAAGTCCGATATGCGG+CGG 0.641711 2:-68091405 MsaT010039.1:CDS
CGGCGTTGTGATCGCCGGTG+AGG 0.644184 2:+68091466 None:intergenic
CATGGGCGTAGGTTACGAGG+AGG 0.653568 2:+68091652 None:intergenic
ATCATCGAACCATCCAAACA+CGG 0.655214 2:+68091446 None:intergenic
TGTGACGGTGGCGGTGACGG+CGG 0.655882 2:+68091581 None:intergenic
TCAAAGGCTCGCCTTATCCG+AGG 0.659522 2:-68095539 MsaT010039.1:intron
AGGAGAGACATATCCACCGT+CGG 0.668578 2:-68095598 MsaT010039.1:CDS
CGGACTTACGAGAACTCCGG+TGG 0.673877 2:+68091416 None:intergenic
TATCGGACTTACGAGAACTC+CGG 0.686902 2:+68091413 None:intergenic
GTTCTCGTAAGTCCGATATG+CGG 0.693669 2:-68091408 MsaT010039.1:CDS
TGTGTCGTTGACGATGCGAG+AGG 0.695536 2:-68091373 MsaT010039.1:CDS
CATCCCGACTTTGACCTCAC+CGG 0.711873 2:-68091480 MsaT010039.1:CDS
CAAGCCCATGAATCCGACGG+TGG 0.711911 2:+68095585 None:intergenic
TCTCCGAGATCGGCAAACAA+CGG 0.712350 2:+68091342 None:intergenic
TCTCGGTCAGCAACATACAG+TGG 0.713476 2:+68096006 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 68091266 68096055 68091266 ID=MsaG010039
Chr2 mRNA 68091266 68096055 68091266 ID=MsaT010039.1;Parent=MsaG010039
Chr2 exon 68091266 68091752 68091266 ID=MsaT010039.1.exon3;Parent=MsaT010039.1
Chr2 CDS 68091266 68091752 68091266 ID=cds.MsaT010039.1;Parent=MsaT010039.1
Chr2 exon 68095540 68095683 68095540 ID=MsaT010039.1.exon2;Parent=MsaT010039.1
Chr2 CDS 68095540 68095683 68095540 ID=cds.MsaT010039.1;Parent=MsaT010039.1
Chr2 exon 68095901 68096055 68095901 ID=MsaT010039.1.exon1;Parent=MsaT010039.1
Chr2 CDS 68095901 68096055 68095901 ID=cds.MsaT010039.1;Parent=MsaT010039.1
Gene Sequence

>MsaT010039.1

ATGTACAAACGTAGATTCAACACTAAACCACTGTATGTTGCTGACCGAGATGAGCCCGAGCCTGAGCTCAAGCTCGAGCCCATCTCAGAAGCGGGGCCTGCTTCTCCCTCTTCTTGTGAAGACACTAAAATTGAAGAACCATCACCCAAGAAAAGGAGGGAGATGAAGAAGAGGGTGGTGACTATACCAATTGCTGACGTGGAAGGATCTAAGAGTAGAGGAGAGACATATCCACCGTCGGATTCATGGGCTTGGAGAAAGTACGGTCAAAAGCCCATCAAAGGCTCGCCTTATCCGAGGGGATACTACCGATGTAGTAGTTCAAAAGGTTGTCCCGCAAGAAAACAAGTCGAAAGAAGCCGTGTAGACCCCACAAACCTCCTCGTAACCTACGCCCATGAACACAACCACTCCCTCCCTCTCCCAAAATCTCATCATTCTTCCACCACCGCCGTCACCGCCACCGTCACAGCTGCCGTCGACACTCCTTCTCCCGGCGAGTCTCCGGCTGCCACTCCTCAGCCGGAAGATCGGCCCGTTTTCGCCACTCATCCCGACTTTGACCTCACCGGCGATCACAACGCCGTGTTTGGATGGTTCGATGATGTTGTATCCACCGGAGTTCTCGTAAGTCCGATATGCGGCGGAGTAGAGGATGTGTCGTTGACGATGCGAGAGGAGGATGAGCCGTTGTTTGCCGATCTCGGAGAGTTGCCGGAGTGTTCAACGGTTTTCCGACAGAGAAATATTCCAAGTGTGTTTCAGTATAGCGGCATTACAGGATAA
Protein sequence

>MsaT010039.1

MYKRRFNTKPLYVADRDEPEPELKLEPISEAGPASPSSCEDTKIEEPSPKKRREMKKRVVTIPIADVEGSKSRGETYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPTNLLVTYAHEHNHSLPLPKSHHSSTTAVTATVTAAVDTPSPGESPAATPQPEDRPVFATHPDFDLTGDHNAVFGWFDDVVSTGVLVSPICGGVEDVSLTMREEDEPLFADLGELPECSTVFRQRNIPSVFQYSGITG*