AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010075


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010075 MtrunA17_Chr2g0320291 97.125 313 6 2 3 315 4 313 0.0 618
MsaG010075 MtrunA17_Chr4g0028071 64.832 327 93 10 2 315 16 333 3.71e-131 376
MsaG010075 MtrunA17_Chr1g0211081 28.936 235 130 6 63 260 239 473 1.17e-20 92.4
MsaG010075 MtrunA17_Chr8g0365711 34.591 159 83 3 127 271 236 387 2.35e-20 90.9
MsaG010075 MtrunA17_Chr3g0131691 30.481 187 108 4 89 260 257 436 3.35e-17 82.0
MsaG010075 MtrunA17_Chr2g0298751 26.203 187 117 3 134 302 51 234 1.53e-16 77.8
MsaG010075 MtrunA17_Chr8g0366751 30.864 162 93 5 139 284 466 624 6.52e-15 75.5
MsaG010075 MtrunA17_Chr4g0048851 31.548 168 108 4 123 289 37 198 1.13e-14 71.6
MsaG010075 MtrunA17_Chr5g0411341 30.488 164 91 5 139 284 497 655 3.74e-14 73.2
MsaG010075 MtrunA17_Chr4g0054571 29.651 172 97 3 98 259 127 284 3.84e-14 72.0
MsaG010075 MtrunA17_Chr7g0275821 30.464 151 97 4 139 288 256 399 6.68e-14 72.0
MsaG010075 MtrunA17_Chr7g0252041 28.859 149 90 2 134 267 285 432 9.95e-14 71.6
MsaG010075 MtrunA17_Chr8g0346151 29.600 125 75 2 111 222 247 371 7.98e-13 68.6
MsaG010075 MtrunA17_Chr4g0049331 26.437 174 121 5 102 271 103 273 1.24e-11 64.3
MsaG010075 MtrunA17_Chr8g0346161 28.421 190 98 9 114 277 259 436 2.16e-11 64.3
MsaG010075 MtrunA17_Chr7g0260841 48.077 52 27 0 139 190 359 410 2.48e-11 64.3
MsaG010075 MtrunA17_Chr1g0153241 31.858 113 64 3 79 187 44 147 2.68e-11 63.2
MsaG010075 MtrunA17_Chr5g0400671 30.435 138 85 4 137 269 156 287 9.91e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010075 AT2G28160.1 52.096 334 120 10 1 315 5 317 6.45e-104 307
MsaG010075 AT2G28160.2 51.524 328 119 10 1 309 5 311 1.42e-99 295
MsaG010075 AT4G29930.6 34.682 173 108 3 134 306 53 220 2.95e-24 99.0
MsaG010075 AT4G29930.2 34.682 173 105 4 134 306 53 217 4.09e-23 95.9
MsaG010075 AT5G57150.1 33.333 192 107 6 123 298 40 226 9.39e-23 95.1
MsaG010075 AT4G29930.5 35.099 151 92 3 134 284 53 197 3.38e-22 93.6
MsaG010075 AT5G57150.2 34.078 179 99 5 123 285 41 216 3.59e-22 93.2
MsaG010075 AT5G57150.5 35.758 165 87 5 123 271 40 201 4.27e-22 92.8
MsaG010075 AT5G57150.3 35.758 165 87 5 123 271 41 202 5.12e-22 92.4
MsaG010075 AT5G57150.4 35.758 165 87 5 123 271 41 202 1.69e-21 92.0
MsaG010075 AT2G16910.1 36.025 161 77 4 133 268 312 471 3.61e-21 94.4
MsaG010075 AT4G29930.1 31.156 199 106 5 134 306 53 246 5.29e-20 87.8
MsaG010075 AT4G29930.3 31.073 177 93 4 134 284 53 226 5.98e-19 85.1
MsaG010075 AT4G29930.7 31.073 177 93 4 134 284 53 226 5.98e-19 85.1
MsaG010075 AT2G46510.1 30.726 179 99 6 120 289 372 534 1.16e-17 84.0
MsaG010075 AT1G01260.1 33.511 188 102 5 95 282 396 560 4.20e-16 79.3
MsaG010075 AT1G01260.2 33.511 188 102 5 95 282 396 560 4.20e-16 79.3
MsaG010075 AT1G01260.3 33.511 188 102 5 95 282 396 560 4.20e-16 79.3
MsaG010075 AT4G29930.4 47.143 70 37 0 134 203 53 122 1.58e-15 73.9
MsaG010075 AT4G17880.1 28.188 149 103 3 139 284 420 567 2.25e-14 73.9
MsaG010075 AT1G12860.2 29.932 147 85 4 134 267 266 407 1.67e-13 71.2
MsaG010075 AT1G12860.1 29.932 147 85 4 134 267 266 407 2.15e-13 70.9
MsaG010075 AT3G26744.6 31.293 147 87 4 134 267 306 451 2.69e-13 70.5
MsaG010075 AT3G26744.1 30.612 147 88 3 134 267 306 451 2.90e-13 70.5
MsaG010075 AT3G26744.4 30.612 147 88 3 134 267 306 451 2.90e-13 70.5
MsaG010075 AT3G26744.2 30.612 147 88 3 134 267 306 451 2.90e-13 70.5
MsaG010075 AT1G32640.1 30.000 150 100 4 139 284 456 604 2.27e-12 68.2
MsaG010075 AT5G46760.1 29.091 165 85 6 139 284 419 570 7.50e-12 66.2
MsaG010075 AT2G22750.1 26.582 158 105 3 128 276 119 274 2.58e-11 63.9
MsaG010075 AT2G22750.5 26.582 158 105 3 128 276 143 298 2.65e-11 63.9
MsaG010075 AT2G22750.4 26.582 158 105 3 128 276 147 302 2.67e-11 63.9
MsaG010075 AT2G22750.3 26.582 158 105 3 128 276 147 302 2.97e-11 63.9
MsaG010075 AT2G22750.2 26.582 158 105 3 128 276 119 274 3.12e-11 63.5
MsaG010075 AT1G10610.3 45.161 62 34 0 133 194 165 226 6.85e-11 62.8
MsaG010075 AT1G10610.2 45.161 62 34 0 133 194 232 293 8.64e-11 62.8
MsaG010075 AT4G37850.2 25.792 221 123 9 61 259 80 281 8.71e-11 62.4
MsaG010075 AT4G37850.1 25.792 221 123 9 61 259 80 281 8.71e-11 62.4

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTGAATGCTGAGGTTTC+TGG 0.189821 2:+68535581 MsaT010075.1:CDS
TGTTATGATGGAGAGGTTAA+AGG 0.231584 2:+68535152 MsaT010075.1:CDS
TATTGTTGTGATCAAATAAT+AGG 0.246719 2:-68535061 None:intergenic
GCTTTATGCATTGCGTTCTT+TGG 0.304101 2:+68535325 MsaT010075.1:CDS
ATGGATAAGGCCTCTATAAT+TGG 0.313859 2:+68535515 MsaT010075.1:CDS
TCGATAAATTGATCAAAGTT+TGG 0.327610 2:-68534930 None:intergenic
GTCATGCAGCTCAAAGTTAT+TGG 0.328211 2:-68534895 None:intergenic
CTAATTACCTTTGTAATGTT+GGG 0.360034 2:-68535350 None:intergenic
ACTAATTACCTTTGTAATGT+TGG 0.365158 2:-68535351 None:intergenic
ACTTCCTAGCTTGTGCTTGA+AGG 0.382012 2:-68535562 None:intergenic
ACCTTCAAAATTTGAAGCTA+TGG 0.386796 2:+68536338 MsaT010075.1:CDS
GGCTCCTTCTCTTGTTATGA+TGG 0.389333 2:+68535140 MsaT010075.1:CDS
GAGCCAATTGTTGTAGGGCT+TGG 0.390918 2:-68535122 None:intergenic
CAATGTTCAAAATTCAAACT+TGG 0.395586 2:+68536145 MsaT010075.1:CDS
TTGTGATCAAATAATAGGTT+TGG 0.409993 2:-68535056 None:intergenic
TTGGAGGAATTGAAAGAAGT+TGG 0.410526 2:-68535037 None:intergenic
CAATTTATCGATTTAATTCG+AGG 0.430612 2:+68534942 MsaT010075.1:CDS
AATCCAAGCCCTACAACAAT+TGG 0.433423 2:+68535119 MsaT010075.1:CDS
GAGGAGGAGAGGCAGGATGA+AGG 0.445153 2:+68535298 MsaT010075.1:CDS
CTGAGAGGAGGAGGAGAGGC+AGG 0.453081 2:+68535291 MsaT010075.1:CDS
GAGAAGGAGCCAATTGTTGT+AGG 0.453789 2:-68535128 None:intergenic
GAAGATGCAATATCTAACTT+TGG 0.471684 2:+68534972 MsaT010075.1:CDS
CCTTCAAAATTTGAAGCTAT+GGG 0.474141 2:+68536339 MsaT010075.1:CDS
CCTCTCAGAAATGAGAGTCT+TGG 0.475407 2:-68535276 None:intergenic
CTCTCATTTCTGAGAGGAGG+AGG 0.478718 2:+68535282 MsaT010075.1:CDS
AATTTGCAAGAAAATTATCC+AGG 0.484959 2:+68535679 MsaT010075.1:CDS
TCAGTGTCTGAAAATTATCA+AGG 0.485823 2:+68535620 MsaT010075.1:CDS
GATGGAGAGGTTAAAGGAGA+AGG 0.491246 2:+68535158 MsaT010075.1:CDS
CAATGAAGCAATCATTGATA+AGG 0.492027 2:-68535001 None:intergenic
TGATCAAATAATAGGTTTGG+AGG 0.499808 2:-68535053 None:intergenic
CTTCTCTTGTTATGATGGAG+AGG 0.500496 2:+68535145 MsaT010075.1:CDS
ATGGCATGAATTCAAAGCCT+TGG 0.500719 2:-68536381 None:intergenic
CATGCAGTGGCTCAAAATGA+TGG 0.501968 2:-68536400 None:intergenic
TACTGCATCTCCAATTATAG+AGG 0.518020 2:-68535525 None:intergenic
AATAACCTCTTTCCTCCACT+TGG 0.518798 2:-68536046 None:intergenic
TATCCACGTACAGATGGATA+AGG 0.530307 2:+68535502 MsaT010075.1:intron
AGAAGGAGCCAATTGTTGTA+GGG 0.535853 2:-68535127 None:intergenic
AAAGGAGAAGGAGAAAATGA+TGG 0.542463 2:+68535170 MsaT010075.1:CDS
GAAGGAGAAAATGATGGAGG+TGG 0.543991 2:+68535176 MsaT010075.1:CDS
CTCTCCATCATAACAAGAGA+AGG 0.545524 2:-68535144 None:intergenic
CAATCGATGATGCTAACCCT+AGG 0.549718 2:+68535237 MsaT010075.1:CDS
ACCCTAGGGCTAAAACTGAT+AGG 0.553134 2:+68535252 MsaT010075.1:CDS
AATTTGAAGCTATGGGTGGC+TGG 0.562736 2:+68536346 MsaT010075.1:CDS
GGTGTTCTCTAGTGAGTCCA+TGG 0.563816 2:-68534874 None:intergenic
ATGACCTTCAAGCACAAGCT+AGG 0.564350 2:+68535558 MsaT010075.1:CDS
ATTTCTGAGAGGAGGAGGAG+AGG 0.573269 2:+68535287 MsaT010075.1:CDS
AGTAGCTGCTTCATTGTACA+AGG 0.575970 2:+68536100 MsaT010075.1:CDS
ATGTTCCAAGTGGAGGAAAG+AGG 0.591850 2:+68536041 MsaT010075.1:CDS
TTTGGTTCCCAACATTACAA+AGG 0.593392 2:+68535343 MsaT010075.1:CDS
AGACTCTCATTTCTGAGAGG+AGG 0.604951 2:+68535279 MsaT010075.1:CDS
GGAGAAGGAGAAAATGATGG+AGG 0.607003 2:+68535173 MsaT010075.1:CDS
AGCTAGGAAGTTGAATGCTG+AGG 0.609235 2:+68535574 MsaT010075.1:CDS
TCAAAATTTGAAGCTATGGG+TGG 0.609799 2:+68536342 MsaT010075.1:CDS
ATATTGTGCAATAAAGGAGA+AGG 0.612090 2:+68536077 MsaT010075.1:CDS
AGGGTTAGCATCATCGATTG+TGG 0.612799 2:-68535234 None:intergenic
GGTGGACATGTTCCAAGTGG+AGG 0.635415 2:+68536034 MsaT010075.1:intron
AATCGATGATGCTAACCCTA+GGG 0.641884 2:+68535238 MsaT010075.1:CDS
CCAAGACTCTCATTTCTGAG+AGG 0.669057 2:+68535276 MsaT010075.1:CDS
GTAGGTGGACATGTTCCAAG+TGG 0.684360 2:+68536031 MsaT010075.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 68534876 68536411 68534876 ID=MsaG010075
Chr2 mRNA 68534876 68536411 68534876 ID=MsaT010075.1;Parent=MsaG010075
Chr2 exon 68534876 68535364 68534876 ID=MsaT010075.1.exon1;Parent=MsaT010075.1
Chr2 CDS 68534876 68535364 68534876 ID=cds.MsaT010075.1;Parent=MsaT010075.1
Chr2 exon 68535515 68535700 68535515 ID=MsaT010075.1.exon2;Parent=MsaT010075.1
Chr2 CDS 68535515 68535700 68535515 ID=cds.MsaT010075.1;Parent=MsaT010075.1
Chr2 exon 68536035 68536208 68536035 ID=MsaT010075.1.exon3;Parent=MsaT010075.1
Chr2 CDS 68536035 68536208 68536035 ID=cds.MsaT010075.1;Parent=MsaT010075.1
Chr2 exon 68536313 68536411 68536313 ID=MsaT010075.1.exon4;Parent=MsaT010075.1
Chr2 CDS 68536313 68536411 68536313 ID=cds.MsaT010075.1;Parent=MsaT010075.1
Gene Sequence

>MsaT010075.1

ATGGACTCACTAGAGAACACCAATAACTTTGAGCTGCATGACTTCATTGACGATCCAAACTTTGATCAATTTATCGATTTAATTCGAGGTGAGCATGAAGATGCAATATCTAACTTTGGCTCTGACCTTATCAATGATTGCTTCATTGATAATAATATTAACCAACTTCTTTCAATTCCTCCAAACCTATTATTTGATCACAACAATAACAATATTATTAATAATAATGTTGTCAATGAGTATAATCCAAGCCCTACAACAATTGGCTCCTTCTCTTGTTATGATGGAGAGGTTAAAGGAGAAGGAGAAAATGATGGAGGTGGTTCTTCTGCAACAACGACGACGACGACGACGACAACCACAATCGATGATGCTAACCCTAGGGCTAAAACTGATAGGTCCAAGACTCTCATTTCTGAGAGGAGGAGGAGAGGCAGGATGAAGGATAAGCTTTATGCATTGCGTTCTTTGGTTCCCAACATTACAAAGATGGATAAGGCCTCTATAATTGGAGATGCAGTATCATATGTGCATGACCTTCAAGCACAAGCTAGGAAGTTGAATGCTGAGGTTTCTGGACTTGAAGCATCTTTATCAGTGTCTGAAAATTATCAAGGATCAATTAGTAACACTATTAATGTTCAAAGCCATCCAATTTGCAAGAAAATTATCCAGGTGGACATGTTCCAAGTGGAGGAAAGAGGTTATTATGCAAAAATATTGTGCAATAAAGGAGAAGGAGTAGCTGCTTCATTGTACAAGGCTCTTGAGTCTCTTGCAAATTTCAATGTTCAAAATTCAAACTTGGCTACAGTTTGTGACACTTTTCTACTTACATTTACATTGAATGTAAACGGTTCTGAACCAGAAATGAACCTTCAAAATTTGAAGCTATGGGTGGCTGGTGCTCTTTTGAACCAAGGCTTTGAATTCATGCCATCATTTTGA

Protein sequence

>MsaT010075.1

MDSLENTNNFELHDFIDDPNFDQFIDLIRGEHEDAISNFGSDLINDCFIDNNINQLLSIPPNLLFDHNNNNIINNNVVNEYNPSPTTIGSFSCYDGEVKGEGENDGGGSSATTTTTTTTTTIDDANPRAKTDRSKTLISERRRRGRMKDKLYALRSLVPNITKMDKASIIGDAVSYVHDLQAQARKLNAEVSGLEASLSVSENYQGSISNTINVQSHPICKKIIQVDMFQVEERGYYAKILCNKGEGVAASLYKALESLANFNVQNSNLATVCDTFLLTFTLNVNGSEPEMNLQNLKLWVAGALLNQGFEFMPSF*