AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010078

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010078 MtrunA17_Chr2g0320341 97.475 396 10 0 1 396 1 396 0.0 815
MsaG010078 MtrunA17_Chr1g0200421 61.350 163 57 2 229 389 236 394 7.84e-71 231
MsaG010078 MtrunA17_Chr8g0382121 56.442 163 66 2 228 389 109 267 1.73e-64 208
MsaG010078 MtrunA17_Chr3g0123941 58.896 163 61 1 227 389 120 276 6.34e-64 207
MsaG010078 MtrunA17_Chr2g0326381 36.129 155 86 3 224 378 41 182 7.31e-26 108
MsaG010078 MtrunA17_Chr1g0185121 34.555 191 110 4 188 378 15 190 5.98e-25 105
MsaG010078 MtrunA17_Chr4g0029521 34.839 155 88 3 224 378 47 188 7.86e-25 105
MsaG010078 MtrunA17_Chr8g0342421 35.484 155 86 3 224 378 71 211 7.94e-25 106
MsaG010078 MtrunA17_Chr4g0053341 37.255 153 87 5 238 388 80 225 8.15e-25 101
MsaG010078 MtrunA17_Chr1g0181381 34.839 155 88 3 224 378 47 188 1.78e-24 105
MsaG010078 MtrunA17_Chr1g0198651 39.024 164 91 8 235 394 105 263 9.49e-24 100
MsaG010078 MtrunA17_Chr1g0210821 36.129 155 85 4 224 378 53 193 1.36e-23 102
MsaG010078 MtrunA17_Chr4g0040571 33.548 155 89 3 224 378 48 188 2.37e-23 101
MsaG010078 MtrunA17_Chr3g0141431 34.194 155 89 3 224 378 31 172 3.13e-23 100
MsaG010078 MtrunA17_Chr2g0330171 34.177 158 90 3 224 381 55 198 4.98e-23 100
MsaG010078 MtrunA17_Chr5g0422571 38.961 154 86 5 236 387 105 252 5.23e-23 98.2
MsaG010078 MtrunA17_Chr7g0263941 34.810 158 89 4 224 381 43 186 5.30e-23 99.8
MsaG010078 MtrunA17_Chr8g0352011 37.584 149 85 4 238 385 173 314 5.55e-23 99.0
MsaG010078 MtrunA17_Chr7g0268961 34.667 150 84 3 229 378 36 171 6.58e-23 100
MsaG010078 MtrunA17_Chr1g0162971 34.810 158 89 4 224 381 50 193 7.10e-23 99.8
MsaG010078 MtrunA17_Chr8g0373541 34.194 155 88 3 224 378 42 182 1.59e-22 99.4
MsaG010078 MtrunA17_Chr7g0258011 36.913 149 87 3 238 386 183 324 2.00e-22 97.4
MsaG010078 MtrunA17_Chr1g0151351 33.548 155 90 3 224 378 50 191 2.32e-22 98.6
MsaG010078 MtrunA17_Chr2g0321291 36.129 155 91 4 235 388 137 284 2.63e-22 96.3
MsaG010078 MtrunA17_Chr1g0191991 33.548 155 89 3 224 378 72 212 2.89e-22 98.6
MsaG010078 MtrunA17_Chr1g0173841 37.584 149 84 4 246 392 230 371 3.06e-22 97.4
MsaG010078 MtrunA17_Chr4g0026521 34.615 156 95 3 231 386 118 266 3.50e-22 95.9
MsaG010078 MtrunA17_Chr1g0199101 34.194 155 88 4 224 378 56 196 3.90e-22 98.2
MsaG010078 MtrunA17_Chr1g0163001 34.839 155 87 4 224 378 52 192 4.78e-22 97.8
MsaG010078 MtrunA17_Chr2g0328921 34.615 156 95 3 231 386 138 286 1.50e-21 94.4
MsaG010078 MtrunA17_Chr8g0375521 33.333 156 90 4 224 378 36 178 3.13e-20 92.0
MsaG010078 MtrunA17_Chr6g0479921 33.333 156 89 5 224 378 38 179 1.21e-19 90.5
MsaG010078 MtrunA17_Chr2g0324991 31.847 157 93 3 227 383 4 146 1.96e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010078 AT5G22890.1 47.207 358 127 7 44 388 53 361 1.19e-109 327
MsaG010078 AT1G34370.3 59.639 166 67 0 224 389 14 179 1.94e-73 231
MsaG010078 AT1G34370.2 36.533 375 192 8 21 389 55 389 9.65e-73 236
MsaG010078 AT1G34370.1 36.533 375 192 8 21 389 55 389 9.65e-73 236
MsaG010078 AT3G45260.2 36.129 155 86 3 224 378 48 189 1.19e-24 105
MsaG010078 AT3G45260.1 36.129 155 86 3 224 378 48 189 1.19e-24 105
MsaG010078 AT5G60470.2 34.839 155 88 4 224 378 53 194 1.40e-24 105
MsaG010078 AT5G60470.3 34.839 155 88 4 224 378 49 190 1.49e-24 105
MsaG010078 AT5G60470.1 35.333 150 84 4 229 378 49 185 2.72e-24 104
MsaG010078 AT5G03150.1 35.484 155 87 3 224 378 62 203 4.61e-24 104
MsaG010078 AT5G44160.1 34.194 155 88 3 224 378 46 186 6.34e-24 103
MsaG010078 AT1G03840.1 33.548 155 89 3 224 378 50 190 8.16e-24 103
MsaG010078 AT1G34790.1 39.490 157 86 5 238 392 138 287 1.24e-23 100
MsaG010078 AT2G02080.1 36.774 155 84 4 224 378 63 203 1.48e-23 102
MsaG010078 AT2G02080.4 36.774 155 84 4 224 378 63 203 1.48e-23 102
MsaG010078 AT2G02080.5 36.774 155 84 4 224 378 63 203 1.48e-23 102
MsaG010078 AT2G02080.3 36.774 155 84 4 224 378 63 203 1.48e-23 102
MsaG010078 AT5G66730.1 35.484 155 86 4 224 378 41 181 2.11e-23 102
MsaG010078 AT1G55110.3 34.839 155 88 3 224 378 72 213 2.46e-23 101
MsaG010078 AT1G55110.1 34.839 155 88 3 224 378 72 213 2.46e-23 101
MsaG010078 AT1G55110.2 34.839 155 88 3 224 378 72 213 2.46e-23 101
MsaG010078 AT2G02070.2 36.129 155 85 4 224 378 61 201 2.76e-23 102
MsaG010078 AT2G02070.1 36.129 155 85 4 224 378 61 201 2.76e-23 102
MsaG010078 AT5G44160.2 34.211 152 86 3 227 378 10 147 2.86e-23 101
MsaG010078 AT1G03840.2 33.553 152 87 3 227 378 51 188 3.07e-23 101
MsaG010078 AT4G02670.2 35.484 155 87 3 224 378 64 205 8.87e-23 99.8
MsaG010078 AT4G02670.1 35.484 155 87 3 224 378 62 203 9.57e-23 99.4
MsaG010078 AT3G13810.6 33.548 155 90 3 224 378 60 201 2.24e-22 99.4
MsaG010078 AT3G13810.5 33.548 155 90 3 224 378 74 215 2.28e-22 99.4
MsaG010078 AT3G13810.4 33.548 155 90 3 224 378 79 220 2.37e-22 99.4
MsaG010078 AT3G13810.1 33.548 155 90 3 224 378 79 220 2.37e-22 99.4
MsaG010078 AT3G50700.1 34.839 155 87 4 224 378 43 183 3.49e-22 98.2
MsaG010078 AT3G13810.3 33.553 152 88 3 227 378 69 207 8.12e-22 97.4
MsaG010078 AT3G13810.2 33.553 152 88 3 227 378 83 221 8.14e-22 97.8
MsaG010078 AT1G14580.1 34.194 155 88 4 224 378 62 202 8.60e-22 97.4
MsaG010078 AT1G14580.2 34.194 155 88 4 224 378 62 202 8.60e-22 97.4
MsaG010078 AT1G14580.3 34.194 155 88 4 224 378 76 216 9.37e-22 97.4
MsaG010078 AT1G51220.1 39.161 143 79 4 246 387 180 315 1.32e-21 95.1
MsaG010078 AT1G08290.1 38.194 144 80 5 246 387 182 318 1.46e-21 95.1
MsaG010078 AT3G20880.1 39.161 143 79 4 246 387 258 393 1.56e-21 95.9
MsaG010078 AT3G57670.1 36.913 149 87 3 238 386 211 352 2.00e-21 95.5
MsaG010078 AT1G13290.1 39.007 141 81 3 246 386 103 238 3.24e-21 93.6
MsaG010078 AT2G01940.1 35.669 157 85 5 224 378 53 195 1.56e-20 93.6
MsaG010078 AT2G02080.6 37.594 133 69 4 246 378 8 126 8.03e-20 91.3
MsaG010078 AT2G02080.2 37.594 133 69 4 246 378 8 126 8.03e-20 91.3
MsaG010078 AT1G25250.3 33.333 156 91 4 224 378 42 185 4.70e-19 88.6
MsaG010078 AT1G25250.1 33.333 153 89 4 227 378 22 162 1.33e-18 87.0
MsaG010078 AT1G68130.1 32.692 156 91 4 224 378 50 192 2.48e-18 86.7
MsaG010078 AT2G01940.3 35.862 145 77 5 224 366 53 183 8.91e-18 85.1

Find 85 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTGCATACATACATTT+TGG 0.081024 2:-68585215 None:intergenic
ACTATCTCATTGTCACATTT+TGG 0.084467 2:-68584896 None:intergenic
GACGATACATGCTTAAAATT+TGG 0.102733 2:+68584851 MsaT010078.1:CDS
TCTAATATTCAATCAAATTT+TGG 0.121082 2:+68584611 MsaT010078.1:CDS
AATTTGGGTCATCTTATAAT+TGG 0.155349 2:+68584867 MsaT010078.1:CDS
TAATCTCTATTCTTAGTAAT+TGG 0.163762 2:-68585061 None:intergenic
AGACCCTCTTATACTATTAT+TGG 0.167733 2:-68584792 None:intergenic
GAAATTCCTTCCATGGAATT+TGG 0.168179 2:-68584317 None:intergenic
TTGACGAGTCTTGTTGATTT+GGG 0.269721 2:-68584436 None:intergenic
TTCTAGGAAAGATAAGCTTA+TGG 0.293207 2:+68585333 MsaT010078.1:CDS
ACGATACATGCTTAAAATTT+GGG 0.323396 2:+68584852 MsaT010078.1:CDS
ATAATAGTAACATTAATTAT+AGG 0.329713 2:+68584720 MsaT010078.1:CDS
GAGATTGAAAAGGAGGGAAT+TGG 0.343144 2:-68584357 None:intergenic
TCTAGGAAAGATAAGCTTAT+GGG 0.346344 2:+68585334 MsaT010078.1:CDS
GTTGACGAGTCTTGTTGATT+TGG 0.361562 2:-68584437 None:intergenic
ACAAGACTCGTCAACATCTA+TGG 0.370241 2:+68584445 MsaT010078.1:CDS
ATTTGGGTCATCTTATAATT+GGG 0.382161 2:+68584868 MsaT010078.1:CDS
TATGACAAACCATTGATAAC+CGG 0.393477 2:-68585385 None:intergenic
TTTCGGTTCTTTCTGATTTG+AGG 0.398405 2:+68585254 MsaT010078.1:CDS
GAAACCAATAATAGTATAAG+AGG 0.400835 2:+68584788 MsaT010078.1:CDS
GTCCTCAAGAAGGGTGTAGA+TGG 0.403741 2:+68585116 MsaT010078.1:CDS
AGAAACATTGTGGTGATCTT+AGG 0.406285 2:+68585284 MsaT010078.1:CDS
AATCAAGTGCAAACTCTTGT+TGG 0.406380 2:+68584401 MsaT010078.1:CDS
AAGCTTATCTTTCCTAGAAA+AGG 0.414705 2:-68585329 None:intergenic
ACACATTATTGTCAAGTTTG+TGG 0.417742 2:+68584953 MsaT010078.1:CDS
TGACAATGAGATAGTTGAAT+TGG 0.426435 2:+68584904 MsaT010078.1:CDS
GGACATGTTGGTTTGTTTGT+GGG 0.428197 2:+68585355 MsaT010078.1:CDS
GGGACATGTTGGTTTGTTTG+TGG 0.441560 2:+68585354 MsaT010078.1:CDS
TACAATGACTTCTTGTATTG+TGG 0.446519 2:-68584486 None:intergenic
TAATAATGAGTCAGAACCAT+TGG 0.457675 2:+68584679 MsaT010078.1:CDS
GAGTAGAGAGATTGAAAAGG+AGG 0.459196 2:-68584364 None:intergenic
TATTGTCAAGTTTGTGGCAA+AGG 0.467134 2:+68584959 MsaT010078.1:CDS
TCAGAACCATTGGATTGGTT+TGG 0.473677 2:+68584689 MsaT010078.1:CDS
ATTGTCAAGTTTGTGGCAAA+GGG 0.476447 2:+68584960 MsaT010078.1:CDS
ATTGAAAAGGAGGGAATTGG+AGG 0.476928 2:-68584354 None:intergenic
TGAACTAGAAATTCCTTCCA+TGG 0.477549 2:-68584324 None:intergenic
GGACATCACCCGGTTATCAA+TGG 0.481764 2:+68585376 MsaT010078.1:CDS
GACACAAACATTGATCTTAG+AGG 0.484429 2:+68584641 MsaT010078.1:CDS
GGGTTTGAAATTGAAAATCA+AGG 0.486940 2:+68584251 MsaT010078.1:CDS
TAATAGAAGTAGTACCTTGA+GGG 0.496518 2:-68584556 None:intergenic
TGAGAGTAGAGAGATTGAAA+AGG 0.497992 2:-68584367 None:intergenic
AGGATGATATTGATGATCAA+AGG 0.500011 2:+68584747 MsaT010078.1:CDS
AGGATGCATATGAGAGCTCA+TGG 0.501614 2:+68585004 MsaT010078.1:CDS
ATGTCAAGGACAACACCTCT+AGG 0.501625 2:+68584230 MsaT010078.1:CDS
GATAAGCTTATGGGACATGT+TGG 0.504366 2:+68585343 MsaT010078.1:CDS
TGACCATAGGAAACATTTGT+AGG 0.507771 2:-68584274 None:intergenic
TTTGTTTGTGGGACATCACC+CGG 0.508232 2:+68585366 MsaT010078.1:CDS
GATGCCATTGCCAAATTCCA+TGG 0.512772 2:+68584307 MsaT010078.1:CDS
TGACTTCAAAGGTTGAAACT+TGG 0.516503 2:-68585152 None:intergenic
ATGAGTCAGAACCATTGGAT+TGG 0.516915 2:+68584684 MsaT010078.1:CDS
AGGCCTACAAATGTTTCCTA+TGG 0.518719 2:+68584271 MsaT010078.1:CDS
CCTTCCATGGAATTTGGCAA+TGG 0.522098 2:-68584311 None:intergenic
GCACAAATCAATGACTTCAA+AGG 0.522700 2:-68585163 None:intergenic
AAATATTCATGTCCTCAAGA+AGG 0.523706 2:+68585106 MsaT010078.1:CDS
AGTAGAGAGATTGAAAAGGA+GGG 0.529561 2:-68584363 None:intergenic
GGATGATATTGATGATCAAA+GGG 0.531710 2:+68584748 MsaT010078.1:CDS
TTGACAATAATGTGTGTACT+TGG 0.534066 2:-68584945 None:intergenic
GGGTCATCTTATAATTGGGT+CGG 0.542248 2:+68584872 MsaT010078.1:CDS
TCAAGAGGGATGCAAATTTG+AGG 0.543405 2:+68584984 MsaT010078.1:CDS
TTGTATTGTGGAGTTCATTG+TGG 0.555990 2:-68584474 None:intergenic
AATATTCATGTCCTCAAGAA+GGG 0.556447 2:+68585107 MsaT010078.1:CDS
TAACATTAATTATAGGCTCA+AGG 0.571276 2:+68584727 MsaT010078.1:CDS
TTCCATCTACACCCTTCTTG+AGG 0.572019 2:-68585118 None:intergenic
GGAAACTAAGAGCAATCTGT+TGG 0.579939 2:-68584535 None:intergenic
CCATTGCCAAATTCCATGGA+AGG 0.581643 2:+68584311 MsaT010078.1:CDS
CTTTCACCAAACCAATCCAA+TGG 0.587496 2:-68584695 None:intergenic
TTTGTGGCAAAGGGTTCAAG+AGG 0.595190 2:+68584969 MsaT010078.1:CDS
ACAAATGTTTCCTATGGTCA+CGG 0.597671 2:+68584277 MsaT010078.1:CDS
ATGACAAACCATTGATAACC+GGG 0.597673 2:-68585384 None:intergenic
CTTAGGTGGCAGTGTTCATG+TGG 0.605201 2:+68585301 MsaT010078.1:CDS
ATCAAATCTTCCGTGACCAT+AGG 0.609192 2:-68584287 None:intergenic
TGTCAAGGACAACACCTCTA+GGG 0.610871 2:+68584231 MsaT010078.1:CDS
AACATTGTGGTGATCTTAGG+TGG 0.610982 2:+68585287 MsaT010078.1:CDS
TGACGAGTCTTGTTGATTTG+GGG 0.614111 2:-68584435 None:intergenic
ATTGCTCTTAGTTTCCCTCA+AGG 0.618106 2:+68584542 MsaT010078.1:CDS
TTAATAGAAGTAGTACCTTG+AGG 0.620130 2:-68584557 None:intergenic
AAACCAATAATAGTATAAGA+GGG 0.620427 2:+68584789 MsaT010078.1:CDS
TTGAAAAGGAGGGAATTGGA+GGG 0.623406 2:-68584353 None:intergenic
GGATGCATATGAGAGCTCAT+GGG 0.624368 2:+68585005 MsaT010078.1:CDS
GCAAGTGAACATCATTGATG+TGG 0.636466 2:-68584513 None:intergenic
CCAAAATGTATGTATGCAAG+AGG 0.650780 2:+68585215 MsaT010078.1:CDS
TGAGATAGTTGAATTGGACG+CGG 0.672074 2:+68584910 MsaT010078.1:CDS
GATGCATATGAGAGCTCATG+GGG 0.685805 2:+68585006 MsaT010078.1:CDS
TTGTGGCAAAGGGTTCAAGA+GGG 0.697728 2:+68584970 MsaT010078.1:CDS
AGGACACATGAGAAACATTG+TGG 0.713628 2:+68585274 MsaT010078.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 68584227 68585426 68584227 ID=MsaG010078
Chr2 mRNA 68584227 68585426 68584227 ID=MsaT010078.1;Parent=MsaG010078
Chr2 exon 68584227 68585426 68584227 ID=MsaT010078.1.exon1;Parent=MsaT010078.1
Chr2 CDS 68584227 68585426 68584227 ID=cds.MsaT010078.1;Parent=MsaT010078.1
Gene Sequence

>MsaT010078.1

ATGATGTCAAGGACAACACCTCTAGGGTTTGAAATTGAAAATCAAGGCCTACAAATGTTTCCTATGGTCACGGAAGATTTGATGCCATTGCCAAATTCCATGGAAGGAATTTCTAGTTCAACTTTACCCTCCAATTCCCTCCTTTTCAATCTCTCTACTCTCAAAGACAAGCTCAATCAAGTGCAAACTCTTGTTGGTGTCATTCTCTCCCCAAATCAACAAGACTCGTCAACATCTATGGCATTATCCACAATGAACTCCACAATACAAGAAGTCATTGTAACTTCCACATCAATGATGTTCACTTGCCAACAGATTGCTCTTAGTTTCCCTCAAGGTACTACTTCTATTAAGTGTACAAACCAAGAATTTCAAAAGCAACAATCTAATATTCAATCAAATTTTGGCAACAATGACACAAACATTGATCTTAGAGGTCAAAGCATATTTTCTAATAATGAGTCAGAACCATTGGATTGGTTTGGTGAAAGCTATAATAGTAACATTAATTATAGGCTCAAGGATGATATTGATGATCAAAGGGTTGATCATGAAATTAGCGAAACCAATAATAGTATAAGAGGGTCTAATGAAGATATTTCAACGAAGAATAATTCTGATCGTGACGATACATGCTTAAAATTTGGGTCATCTTATAATTGGGTCGGCCCAAAATGTGACAATGAGATAGTTGAATTGGACGCGGCTGATTTATTAGCCAAGTACACACATTATTGTCAAGTTTGTGGCAAAGGGTTCAAGAGGGATGCAAATTTGAGGATGCATATGAGAGCTCATGGGGATGAGTACAAGAGCAGTGCAGCATTGAGCAATCCAATTACTAAGAATAGAGATTATTTGATGAGTATGAAAAATAAGAAATATTCATGTCCTCAAGAAGGGTGTAGATGGAACCAAAAACATGCCAAGTTTCAACCTTTGAAGTCATTGATTTGTGCTAAGAATCATTACAAGAGAAGTCATTGTCCCAAAATGTATGTATGCAAGAGGTGTAATTTGAAGCAATTTTCGGTTCTTTCTGATTTGAGGACACATGAGAAACATTGTGGTGATCTTAGGTGGCAGTGTTCATGTGGCACTACCTTTTCTAGGAAAGATAAGCTTATGGGACATGTTGGTTTGTTTGTGGGACATCACCCGGTTATCAATGGTTTGTCATATAGTACTCTCACAAGTTAG
Protein sequence

>MsaT010078.1

MMSRTTPLGFEIENQGLQMFPMVTEDLMPLPNSMEGISSSTLPSNSLLFNLSTLKDKLNQVQTLVGVILSPNQQDSSTSMALSTMNSTIQEVIVTSTSMMFTCQQIALSFPQGTTSIKCTNQEFQKQQSNIQSNFGNNDTNIDLRGQSIFSNNESEPLDWFGESYNSNINYRLKDDIDDQRVDHEISETNNSIRGSNEDISTKNNSDRDDTCLKFGSSYNWVGPKCDNEIVELDAADLLAKYTHYCQVCGKGFKRDANLRMHMRAHGDEYKSSAALSNPITKNRDYLMSMKNKKYSCPQEGCRWNQKHAKFQPLKSLICAKNHYKRSHCPKMYVCKRCNLKQFSVLSDLRTHEKHCGDLRWQCSCGTTFSRKDKLMGHVGLFVGHHPVINGLSYSTLTS*