AlfalfaGEDB | MsaG010145

M. sativa ssp. caerulea / MsaG010145

KEGG: K11276
GO: -
PFAM: PF17800.4

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010145	XP_013464908.1	81.046	306	26	5	1	278	1	302	5.84e-143	414

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010145	sp\|Q8LJS2\|HDT1_SOYBN	59.934	302	90	9	1	278	1	295	1.59e-96	288

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010145	tr\|I3SZA0\|I3SZA0_MEDTR	81.046	306	26	5	1	278	1	302	2.79e-143	414

TFs/TRs:

Gene ID	Type	Classification
MsaG010145	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG004860	MsaG010145	0.836721	1.450921e-56	5.117700e-54
MsaG006934	MsaG010145	0.805487	2.205386e-49	3.381417e-47
MsaG007483	MsaG010145	0.818209	3.851675e-52	8.082848e-50
MsaG010145	MsaG011891	0.802711	8.282899e-49	1.190928e-46
MsaG010145	MsaG016489	0.808856	4.298124e-50	7.140396e-48
MsaG010145	MsaG017733	0.809105	3.803431e-50	6.356588e-48
MsaG010145	MsaG018483	0.804117	4.248740e-49	6.310897e-47
MsaG010145	MsaG018781	0.804255	3.977814e-49	5.927446e-47
MsaG010145	MsaG020532	0.808237	5.819605e-50	9.524742e-48
MsaG010145	MsaG023698	0.811316	1.276087e-50	2.250376e-48
MsaG010145	MsaG023939	0.802226	1.041593e-48	1.481098e-46
MsaG010145	MsaG027861	0.810632	1.791621e-50	3.107086e-48
MsaG010145	MsaG030479	0.820095	1.439808e-52	3.174746e-50
MsaG010145	MsaG030474	0.811245	1.321955e-50	2.327197e-48
MsaG010145	MsaG031531	0.802281	1.015064e-48	1.445195e-46
MsaG010145	MsaG035534	0.801613	1.389699e-48	1.948730e-46
MsaG010145	MsaG040358	0.813372	4.559200e-51	8.460190e-49
MsaG010145	MsaG044372	0.802829	7.834723e-49	1.129529e-46
MsaG010145	MsaG033035	0.804059	4.367492e-49	6.478742e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010145	MtrunA17_Chr2g0320884	82.609	299	27	5	1	278	35	329	6.59e-144	407
MsaG010145	MtrunA17_Chr4g0027181	47.773	247	116	5	1	244	5	241	6.04e-55	178
MsaG010145	MtrunA17_Chr7g0265761	42.208	308	129	8	1	278	1	289	6.81e-49	163
MsaG010145	MtrunA17_Chr4g0027191	45.274	201	91	3	2	186	6	203	2.57e-38	138
MsaG010145	MtrunA17_Chr4g0027191	53.125	96	34	4	183	275	282	369	1.50e-14	72.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010145	AT5G03740.1	41.311	305	138	10	1	277	1	292	6.26e-46	156
MsaG010145	AT5G22650.2	40.753	292	136	9	1	263	1	284	8.15e-36	130
MsaG010145	AT5G22650.1	40.614	293	136	10	1	263	1	285	4.58e-34	125
MsaG010145	AT3G44750.2	50.000	92	43	2	1	92	1	89	6.56e-23	94.7
MsaG010145	AT3G44750.1	50.000	92	43	2	1	92	1	89	6.96e-23	94.7
MsaG010145	AT2G27840.4	42.222	90	47	3	1	88	1	87	1.52e-15	72.8
MsaG010145	AT2G27840.3	42.222	90	47	3	1	88	1	87	1.73e-15	73.6
MsaG010145	AT2G27840.1	42.222	90	47	3	1	88	1	87	2.34e-15	72.0

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTGTTTCTAATGGAGTTT+TGG	0.129448	2:+69235761	None:intergenic
TCCTCCAGACTTGGGAGTTT+TGG	0.188316	2:-69238498	None:intergenic
GGAGTCTTTCCACCTTTCTT+AGG	0.193593	2:-69238464	None:intergenic
GAAACTTTCTCCTCTGCTTT+TGG	0.244330	2:-69237107	None:intergenic
GAAAGTTTCTGAACCTAAAA+AGG	0.272635	2:+69237121	MsaT010145.1:CDS
GATGAATCTGATGATGAAAA+TGG	0.302701	2:+69237230	MsaT010145.1:CDS
TGGGGAACAAAAGCTTGTTT+TGG	0.309523	2:+69236296	MsaT010145.1:CDS
TTTGATTTCTTGGTAGAAAC+TGG	0.315658	2:-69237744	None:intergenic
CATTGGGCCTCTTCTTTCCC+TGG	0.324129	2:-69237704	None:intergenic
TCTTCATCTTCCTCATCCTT+TGG	0.331069	2:-69237191	None:intergenic
TGAATTAGGTGCTGAGGTTA+AGG	0.360277	2:+69236060	MsaT010145.1:intron
TTTAGCAGGTGCAGCATTCT+TGG	0.365068	2:-69237150	None:intergenic
AGGACATTCAACTCTGAAAC+TGG	0.374828	2:+69238656	MsaT010145.1:intron
TGGTTATTTACCTCAAAGTT+GGG	0.376973	2:+69236277	MsaT010145.1:CDS
TTCTGCTCTGTGTTAATCAA+TGG	0.388646	2:-69236978	None:intergenic
GCAGCTGCATCAAGGTCATA+TGG	0.393293	2:-69236106	None:intergenic
GAGTCTTTCCACCTTTCTTA+GGG	0.394889	2:-69238463	None:intergenic
GAAATGAACACTAGCAGCTT+TGG	0.398995	2:-69236397	None:intergenic
TAAATAACCACAGGCTCGTT+TGG	0.405456	2:-69236264	None:intergenic
GTGGTTATTTACCTCAAAGT+TGG	0.423882	2:+69236276	MsaT010145.1:CDS
GATGCCAAAACTCCCAAGTC+TGG	0.432291	2:+69238494	MsaT010145.1:CDS
TTGGTCTCACCAGTCTTCTC+AGG	0.439494	2:-69237777	None:intergenic
AGCTGCTATTCACATTTCTC+AGG	0.449259	2:+69236123	MsaT010145.1:CDS
TCTTGATATTGTCCTTGAAA+AGG	0.456315	2:+69236353	MsaT010145.1:CDS
TTGTTAGATGGTAAGAAAGC+TGG	0.456417	2:+69238422	MsaT010145.1:intron
TTAGGTGCTGAGGTTAAGGT+TGG	0.459431	2:+69236064	MsaT010145.1:intron
AGAGAGTCCTCCTCCAGACT+TGG	0.471298	2:-69238507	None:intergenic
TTTCCACCTTTCTTAGGGTG+AGG	0.472414	2:-69238458	None:intergenic
ACTTCACTTAGCAGCATGCT+TGG	0.492494	2:-69238699	None:intergenic
TCTGCTCTGTGTTAATCAAT+GGG	0.495154	2:-69236977	None:intergenic
ACAACCTTCACGTGTTTAGC+AGG	0.509884	2:-69237164	None:intergenic
GGAAGAGACTCCTGTGAAGA+AGG	0.527324	2:+69237587	MsaT010145.1:CDS
TGTGCCATTACCTTCTTCAC+AGG	0.535491	2:-69237597	None:intergenic
CAGCTGCATCAAGGTCATAT+GGG	0.546249	2:-69236105	None:intergenic
TGAGAGTGATGAGGATGATG+AGG	0.559141	2:+69237566	MsaT010145.1:CDS
GAGAGTCCTCCTCCAGACTT+GGG	0.565423	2:-69238506	None:intergenic
AATGCTACTCCTGAGAAGAC+TGG	0.569825	2:+69237768	MsaT010145.1:CDS
GCAACTCCTCACCCTAAGAA+AGG	0.575686	2:+69238452	MsaT010145.1:CDS
GGTTGTTGATCCAAAGGATG+AGG	0.576616	2:+69237181	MsaT010145.1:CDS
CGTGAAGGTTGTTGATCCAA+AGG	0.578956	2:+69237175	MsaT010145.1:CDS
GTTATTGTATAGGTTGCACT+TGG	0.583377	2:+69236222	MsaT010145.1:intron
AAAACTCCCAAGTCTGGAGG+AGG	0.583660	2:+69238500	MsaT010145.1:CDS
TGTGAATAGCAGCTGCATCA+AGG	0.595728	2:-69236114	None:intergenic
TTGATTAACACAGAGCAGAA+TGG	0.601793	2:+69236981	MsaT010145.1:CDS
GGTTATTTACCTCAAAGTTG+GGG	0.606552	2:+69236278	MsaT010145.1:CDS
AAAACCAGAACCAAAAGCAG+AGG	0.625041	2:+69237097	MsaT010145.1:CDS
TGGACTGCAACAACATAGCA+AGG	0.629185	2:+69238676	MsaT010145.1:CDS
ACTCCTCACCCTAAGAAAGG+TGG	0.633029	2:+69238455	MsaT010145.1:CDS
AAATGGAAGCTCTGATGATG+AGG	0.649458	2:+69237247	MsaT010145.1:CDS
TGCACCTGCTAAACACGTGA+AGG	0.650932	2:+69237160	MsaT010145.1:CDS
GCCAAAACTCCCAAGTCTGG+AGG	0.652249	2:+69238497	MsaT010145.1:CDS
ACTTTGAGGTAAATAACCAC+AGG	0.655718	2:-69236273	None:intergenic
AGACAAACCAAACGAGCCTG+TGG	0.660313	2:+69236257	MsaT010145.1:CDS
ACAATATCAAGAGAAATCTG+AGG	0.664546	2:-69236342	None:intergenic
AGATGATGATGAGAGTGATG+AGG	0.674103	2:+69237557	MsaT010145.1:CDS
AGGTTGACCAGGGAAAGAAG+AGG	0.740019	2:+69237697	MsaT010145.1:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	69235772	69238718	69235772	ID=MsaG010145
Chr2	mRNA	69235772	69238718	69235772	ID=MsaT010145.1;Parent=MsaG010145
Chr2	exon	69235772	69235784	69235772	ID=MsaT010145.1.exon1;Parent=MsaT010145.1
Chr2	CDS	69235772	69235784	69235772	ID=cds.MsaT010145.1;Parent=MsaT010145.1
Chr2	exon	69236068	69236144	69236068	ID=MsaT010145.1.exon2;Parent=MsaT010145.1
Chr2	CDS	69236068	69236144	69236068	ID=cds.MsaT010145.1;Parent=MsaT010145.1
Chr2	exon	69236234	69236420	69236234	ID=MsaT010145.1.exon3;Parent=MsaT010145.1
Chr2	CDS	69236234	69236420	69236234	ID=cds.MsaT010145.1;Parent=MsaT010145.1
Chr2	exon	69236958	69237002	69236958	ID=MsaT010145.1.exon4;Parent=MsaT010145.1
Chr2	CDS	69236958	69237002	69236958	ID=cds.MsaT010145.1;Parent=MsaT010145.1
Chr2	exon	69237096	69237268	69237096	ID=MsaT010145.1.exon5;Parent=MsaT010145.1
Chr2	CDS	69237096	69237268	69237096	ID=cds.MsaT010145.1;Parent=MsaT010145.1
Chr2	exon	69237537	69237608	69237537	ID=MsaT010145.1.exon6;Parent=MsaT010145.1
Chr2	CDS	69237537	69237608	69237537	ID=cds.MsaT010145.1;Parent=MsaT010145.1
Chr2	exon	69237699	69237789	69237699	ID=MsaT010145.1.exon7;Parent=MsaT010145.1
Chr2	CDS	69237699	69237789	69237699	ID=cds.MsaT010145.1;Parent=MsaT010145.1
Chr2	exon	69238429	69238546	69238429	ID=MsaT010145.1.exon8;Parent=MsaT010145.1
Chr2	CDS	69238429	69238546	69238429	ID=cds.MsaT010145.1;Parent=MsaT010145.1
Chr2	exon	69238658	69238718	69238658	ID=MsaT010145.1.exon9;Parent=MsaT010145.1
Chr2	CDS	69238658	69238718	69238658	ID=cds.MsaT010145.1;Parent=MsaT010145.1

Gene Sequence

>MsaT010145.1

ATGGAGTTTTGGGGTGCTGAGGTTAAGGTTGGAGAAACTATCAAGATTGACCCATATGACCTTGATGCAGCTGCTATTCACATTTCTCAGGTTGCACTTGGTGAAGCAAAGAAAGACAAACCAAACGAGCCTGTGGTTATTTACCTCAAAGTTGGGGAACAAAAGCTTGTTTTGGGAACCCTAAACAGGGACAAAATTCCTCAGATTTCTCTTGATATTGTCCTTGAAAAGGAGTTTGAGCTATCTCACAATTCCAAAGCTGCTAGTGTTCATTTCTATAGTGAAGATGAAGACATCCCATTGATTAACACAGAGCAGAATGGAAAACCAGAACCAAAAGCAGAGGAGAAAGTTTCTGAACCTAAAAAGGCTGATGCCAAGAATGCTGCACCTGCTAAACACGTGAAGGTTGTTGATCCAAAGGATGAGGAAGATGAAGATTCTGATGATGATTCTGATGAATCTGATGATGAAAATGGAAGCTCTGATGATGAGATGGAGAATGCTGATAGTGAAGATGATGATGAGAGTGATGAGGATGATGAGGAAGAGACTCCTGTGAAGAAGGTTGACCAGGGAAAGAAGAGGCCCAATGAATCTGCTTCAAAAACTCCAGTTTCTACCAAGAAATCAAAAAATGCTACTCCTGAGAAGACTGATGGTAAGAAAGCTGGACATACAGCAACTCCTCACCCTAAGAAAGGTGGAAAGACTCCTAACAGTGATGCCAAAACTCCCAAGTCTGGAGGAGGACTCTCTTGCAGTAGTTGCAGCAAGACATTCAACTCTGAAACTGGACTGCAACAACATAGCAAGGCCAAGCATGCTGCTAAGTGA

Protein sequence

>MsaT010145.1

MEFWGAEVKVGETIKIDPYDLDAAAIHISQVALGEAKKDKPNEPVVIYLKVGEQKLVLGTLNRDKIPQISLDIVLEKEFELSHNSKAASVHFYSEDEDIPLINTEQNGKPEPKAEEKVSEPKKADAKNAAPAKHVKVVDPKDEEDEDSDDDSDESDDENGSSDDEMENADSEDDDESDEDDEEETPVKKVDQGKKRPNESASKTPVSTKKSKNATPEKTDGKKAGHTATPHPKKGGKTPNSDAKTPKSGGGLSCSSCSKTFNSETGLQQHSKAKHAAK*