AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010184

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010184 MtrunA17_Chr2g0321381 92.708 288 20 1 1 288 1 287 0.0 525
MsaG010184 MtrunA17_Chr2g0321411 67.893 299 62 5 1 298 1 266 4.83e-138 392
MsaG010184 MtrunA17_Chr8g0355121 37.676 284 130 11 25 285 26 285 7.67e-37 134
MsaG010184 MtrunA17_Chr3g0080341 37.884 293 140 15 20 285 17 294 1.48e-35 131
MsaG010184 MtrunA17_Chr7g0254991 33.546 313 143 12 12 286 8 293 2.19e-32 122
MsaG010184 MtrunA17_Chr7g0266211 47.761 134 57 4 29 149 45 178 2.42e-29 116
MsaG010184 MtrunA17_Chr7g0266211 43.373 166 63 4 168 317 272 422 9.37e-21 92.4
MsaG010184 MtrunA17_Chr6g0460301 36.271 295 137 12 24 285 17 293 3.22e-25 103
MsaG010184 MtrunA17_Chr3g0133971 42.748 131 60 6 24 151 28 146 4.66e-20 90.5
MsaG010184 MtrunA17_Chr3g0133971 68.421 57 18 0 229 285 339 395 8.17e-16 77.8
MsaG010184 MtrunA17_Chr4g0044771 34.559 136 69 4 24 151 19 142 5.75e-15 75.1
MsaG010184 MtrunA17_Chr4g0044771 73.469 49 13 0 241 289 300 348 9.12e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010184 AT3G44460.1 43.574 319 149 9 7 311 16 317 1.60e-60 197
MsaG010184 AT2G36270.3 35.369 393 126 10 20 298 34 412 2.70e-55 186
MsaG010184 AT2G36270.1 35.204 392 126 10 20 297 34 411 4.72e-55 186
MsaG010184 AT2G36270.2 35.204 392 126 10 20 297 34 411 4.72e-55 186
MsaG010184 AT3G56850.1 39.427 279 124 12 25 285 18 269 2.43e-40 143
MsaG010184 AT2G41070.5 34.211 266 125 7 24 286 17 235 9.10e-35 127
MsaG010184 AT2G41070.3 34.211 266 125 7 24 286 17 235 1.53e-34 127
MsaG010184 AT2G41070.1 34.211 266 125 7 24 286 17 235 1.53e-34 127
MsaG010184 AT2G41070.2 34.211 266 125 7 24 286 17 235 1.53e-34 127
MsaG010184 AT2G41070.4 34.211 266 125 7 24 286 26 244 1.95e-34 127
MsaG010184 AT1G45249.3 29.730 370 152 12 24 285 22 391 2.80e-27 111
MsaG010184 AT1G49720.2 39.568 139 69 5 21 149 20 153 5.52e-20 90.5
MsaG010184 AT1G49720.2 62.903 62 22 1 240 300 310 371 1.44e-14 74.3
MsaG010184 AT1G49720.1 39.568 139 69 5 21 149 20 153 7.12e-20 89.7
MsaG010184 AT1G49720.1 72.000 50 14 0 240 289 310 359 5.25e-14 72.8
MsaG010184 AT1G49720.3 39.568 139 69 5 21 149 20 153 7.12e-20 89.7
MsaG010184 AT1G49720.3 72.000 50 14 0 240 289 310 359 5.25e-14 72.8
MsaG010184 AT1G45249.7 35.294 153 88 2 24 165 37 189 9.33e-20 89.7
MsaG010184 AT1G45249.7 64.516 62 19 1 224 285 337 395 3.66e-15 76.3
MsaG010184 AT1G45249.6 35.294 153 88 2 24 165 37 189 9.33e-20 89.7
MsaG010184 AT1G45249.6 64.516 62 19 1 224 285 337 395 3.66e-15 76.3
MsaG010184 AT1G45249.1 35.294 153 88 2 24 165 22 174 1.03e-19 89.7
MsaG010184 AT1G45249.1 64.516 62 19 1 224 285 322 380 2.91e-15 76.6
MsaG010184 AT1G45249.10 35.294 153 88 2 24 165 22 174 2.01e-19 88.6
MsaG010184 AT1G45249.5 35.294 153 88 2 24 165 22 174 2.01e-19 88.6
MsaG010184 AT1G45249.4 35.294 153 88 2 24 165 22 174 2.38e-19 88.6
MsaG010184 AT1G45249.8 35.294 153 88 2 24 165 22 174 2.38e-19 88.6
MsaG010184 AT1G45249.2 35.294 153 88 2 24 165 22 174 2.38e-19 88.6
MsaG010184 AT1G45249.9 35.294 153 88 2 24 165 22 174 2.38e-19 88.6
MsaG010184 AT4G34000.3 31.532 222 96 6 24 201 28 237 6.41e-18 84.7
MsaG010184 AT4G34000.3 55.696 79 25 3 240 308 371 449 9.45e-15 75.1
MsaG010184 AT4G34000.1 31.532 222 96 6 24 201 28 237 7.45e-18 84.3
MsaG010184 AT4G34000.1 62.712 59 22 0 240 298 371 429 1.50e-14 74.7
MsaG010184 AT4G34000.4 31.532 222 96 6 24 201 28 237 7.45e-18 84.3
MsaG010184 AT4G34000.4 62.712 59 22 0 240 298 371 429 1.50e-14 74.7
MsaG010184 AT4G34000.2 31.532 222 96 6 24 201 28 237 7.45e-18 84.3
MsaG010184 AT4G34000.2 62.712 59 22 0 240 298 371 429 1.50e-14 74.7
MsaG010184 AT3G19290.4 37.063 143 77 4 24 153 35 177 1.09e-16 80.9
MsaG010184 AT3G19290.4 46.847 111 47 2 194 298 304 408 8.96e-16 78.2
MsaG010184 AT3G19290.1 37.063 143 77 4 24 153 35 177 1.41e-16 80.5
MsaG010184 AT3G19290.1 45.946 111 48 2 194 298 304 408 3.40e-15 76.3
MsaG010184 AT3G19290.5 37.063 143 77 4 24 153 35 177 1.41e-16 80.5
MsaG010184 AT3G19290.5 45.946 111 48 2 194 298 304 408 3.40e-15 76.3
MsaG010184 AT3G19290.2 37.063 143 77 4 24 153 35 177 1.44e-16 80.5
MsaG010184 AT3G19290.3 37.063 143 77 4 24 153 35 177 1.51e-16 80.5
MsaG010184 AT3G19290.3 46.364 110 47 2 194 297 304 407 4.89e-15 75.9
MsaG010184 AT5G44080.1 32.836 201 96 7 98 296 120 283 4.76e-16 78.2
MsaG010184 AT2G17770.2 31.016 187 107 6 100 284 1 167 1.12e-12 66.2
MsaG010184 AT5G42910.1 38.095 126 55 5 24 146 21 126 1.17e-12 68.6
MsaG010184 AT1G03970.1 30.457 197 96 5 98 294 85 240 1.22e-11 64.7

Find 66 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCACCAGGTTGTGCTTAT+AGG 0.185713 2:-69774231 None:intergenic
AATCATAATGGCAATGTTTC+AGG 0.221412 2:+69773200 MsaT010184.1:CDS
TCTCTGTCCCTCCTCCAATT+TGG 0.252757 2:+69772997 MsaT010184.1:CDS
TTGAATATTGCAAGTTGTTA+TGG 0.294474 2:+69773236 MsaT010184.1:CDS
AATCAAGTGGGTGTAGTAAT+AGG 0.330386 2:+69773396 MsaT010184.1:CDS
TGAAAATCTAAAACAAGTTC+TGG 0.355048 2:+69773891 MsaT010184.1:CDS
GTCATTGAGGCTTGTTCACC+AGG 0.361408 2:-69774244 None:intergenic
AACACTTGGAGAAATGAGTT+TGG 0.378783 2:+69773121 MsaT010184.1:CDS
GCATTCAACAAAGGCTCAAA+AGG 0.385055 2:+69774179 MsaT010184.1:CDS
TGTTATTCAGTGACAACATT+TGG 0.394761 2:-69773325 None:intergenic
ACTTACCTCTTGTTTCCTCT+TGG 0.402763 2:-69774041 None:intergenic
AGAATGAATTACCAAGTTGT+TGG 0.415586 2:-69772977 None:intergenic
AGCTCTTTCCACTCTTGCAT+TGG 0.422494 2:-69772842 None:intergenic
GAGGATTTCTTAGTAAAAGC+TGG 0.422923 2:+69773143 MsaT010184.1:CDS
TGCAAGAGTGGAAAGAGCTT+TGG 0.423243 2:+69772846 MsaT010184.1:CDS
ATGAATTCCTTGCCAGCATA+TGG 0.439595 2:+69772883 MsaT010184.1:CDS
TTGCAAGTTGTTATGGTTTG+AGG 0.440862 2:+69773243 MsaT010184.1:CDS
GAATGAATTACCAAGTTGTT+GGG 0.445076 2:-69772976 None:intergenic
TCTTGCATTGGAACTCATCA+AGG 0.446472 2:-69772830 None:intergenic
TTGCCATTCAAGAATCATAA+TGG 0.446766 2:+69773188 MsaT010184.1:CDS
TTGCCATTATGATTCTTGAA+TGG 0.449693 2:-69773191 None:intergenic
ACTCAATGTGTGTCTAGAAA+TGG 0.449816 2:+69773290 MsaT010184.1:CDS
TTATGGTTTGAGGCCTTCAA+TGG 0.451042 2:+69773253 MsaT010184.1:CDS
GAAAATCCCATTCCCATTGA+AGG 0.469209 2:-69773266 None:intergenic
AGAAAACTGTGGATGAAGTT+TGG 0.470434 2:+69773021 MsaT010184.1:CDS
TTGAGGCCTTCAATGGGAAT+GGG 0.472705 2:+69773260 MsaT010184.1:CDS
CTCAAGAAGCAACAAACACT+TGG 0.475735 2:+69773107 MsaT010184.1:CDS
TTATTTGTTTCTTTGAATTG+TGG 0.479374 2:-69773065 None:intergenic
CACACCGTTTCCCAACAACT+TGG 0.481051 2:+69772966 MsaT010184.1:CDS
GCTTCTGCTTGTTCTTGCAA+TGG 0.484450 2:-69772762 None:intergenic
TTTGAGGCCTTCAATGGGAA+TGG 0.489129 2:+69773259 MsaT010184.1:CDS
ATTACCAAGTTGTTGGGAAA+CGG 0.492082 2:-69772970 None:intergenic
TTGTTTCTTTGAATTGTGGT+TGG 0.500429 2:-69773061 None:intergenic
CTTCGCTCCACTACAACATC+AGG 0.510329 2:-69773440 None:intergenic
TAATAGGAAGAGGATAATTG+AGG 0.511054 2:+69773412 MsaT010184.1:CDS
GTCATTAGAACTCCATATGC+TGG 0.516405 2:-69772895 None:intergenic
GTGGGTGTAGTAATAGGAAG+AGG 0.522464 2:+69773402 MsaT010184.1:CDS
ACTTGGAGAAATGAGTTTGG+AGG 0.522848 2:+69773124 MsaT010184.1:CDS
AAGGAATTCATCCATGTTGA+GGG 0.524720 2:-69772871 None:intergenic
ATTGTCAAATCTTGTCATTG+AGG 0.525608 2:-69774257 None:intergenic
TAACAACTTGCAATATTCAA+TGG 0.527054 2:-69773233 None:intergenic
AATAGGAAGAGGATAATTGA+GGG 0.535141 2:+69773413 MsaT010184.1:CDS
ATCTAATTGTTGTAGAGCCA+TGG 0.537356 2:-69772730 None:intergenic
TATGGTTTGAGGCCTTCAAT+GGG 0.547796 2:+69773254 MsaT010184.1:CDS
TCGTTCTCGAGCAAGAAGAC+AGG 0.556810 2:+69773499 MsaT010184.1:CDS
TTGTCACTGAATAACAACTT+AGG 0.558145 2:+69773332 MsaT010184.1:CDS
GAGCTTTGGTTCCCTCAACA+TGG 0.562617 2:+69772860 MsaT010184.1:CDS
CATTCAACAAAGGCTCAAAA+GGG 0.564482 2:+69774180 MsaT010184.1:CDS
GGCATATACATTAGAGCTAG+AGG 0.565644 2:+69773840 MsaT010184.1:intron
CAAGGAATTCATCCATGTTG+AGG 0.566750 2:-69772872 None:intergenic
TAGAACTCCATATGCTGGCA+AGG 0.571014 2:-69772890 None:intergenic
TCTACAACAATTAGATCAAG+AGG 0.593387 2:+69772737 MsaT010184.1:CDS
TCCAATTTGGAAGAAAACTG+TGG 0.593419 2:+69773010 MsaT010184.1:CDS
CTGATGTTGTAGTGGAGCGA+AGG 0.596622 2:+69773441 MsaT010184.1:CDS
GCAACGAAAGCATTCAACAA+AGG 0.598492 2:+69774170 MsaT010184.1:CDS
GATGAGTTCCAATGCAAGAG+TGG 0.607955 2:+69772834 MsaT010184.1:CDS
GGGTCCTCCTGATGTTGTAG+TGG 0.611715 2:+69773433 MsaT010184.1:CDS
GAATAACAACTTAGGAGTGA+AGG 0.616648 2:+69773340 MsaT010184.1:CDS
CTTGCATTGGAACTCATCAA+GGG 0.629128 2:-69772829 None:intergenic
CGCTCCACTACAACATCAGG+AGG 0.642123 2:-69773437 None:intergenic
AGGCTGAAGCTGAGACCAAG+AGG 0.647700 2:+69774026 MsaT010184.1:intron
TGAGACCAAGAGGAAACAAG+AGG 0.662863 2:+69774036 MsaT010184.1:CDS
GAAGGCCTATAAGCACAACC+TGG 0.668432 2:+69774226 MsaT010184.1:CDS
CAAAGCTTGACTGAATCAAG+TGG 0.677316 2:+69773383 MsaT010184.1:CDS
AGGAGTGAAGGAATGTGCTG+TGG 0.702551 2:+69773352 MsaT010184.1:CDS
AAAGCTTGACTGAATCAAGT+GGG 0.724655 2:+69773384 MsaT010184.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 69772732 69774265 69772732 ID=MsaG010184
Chr2 mRNA 69772732 69774265 69772732 ID=MsaT010184.1;Parent=MsaG010184
Chr2 exon 69772732 69773520 69772732 ID=MsaT010184.1.exon1;Parent=MsaT010184.1
Chr2 CDS 69772732 69773520 69772732 ID=cds.MsaT010184.1;Parent=MsaT010184.1
Chr2 exon 69773841 69773912 69773841 ID=MsaT010184.1.exon2;Parent=MsaT010184.1
Chr2 CDS 69773841 69773912 69773841 ID=cds.MsaT010184.1;Parent=MsaT010184.1
Chr2 exon 69774028 69774061 69774028 ID=MsaT010184.1.exon3;Parent=MsaT010184.1
Chr2 CDS 69774028 69774061 69774028 ID=cds.MsaT010184.1;Parent=MsaT010184.1
Chr2 exon 69774165 69774265 69774165 ID=MsaT010184.1.exon4;Parent=MsaT010184.1
Chr2 CDS 69774165 69774265 69774165 ID=cds.MsaT010184.1;Parent=MsaT010184.1
Gene Sequence

>MsaT010184.1

ATGGCTCTACAACAATTAGATCAAGAGGTTCCATTGCAAGAACAAGCAGAAGCTACATTTTCTCAACCAAGCAAACAAAACTCAATACTCTCACTCACCCTTGATGAGTTCCAATGCAAGAGTGGAAAGAGCTTTGGTTCCCTCAACATGGATGAATTCCTTGCCAGCATATGGAGTTCTAATGACGAAGCTACAACTCAAACTCAAAATGCTAAAAATGTTGCAACAACGGAACACACCGTTTCCCAACAACTTGGTAATTCATTCTCTGTCCCTCCTCCAATTTGGAAGAAAACTGTGGATGAAGTTTGGTCTGAGATTCATAAAAACCAACCACAATTCAAAGAAACAAATAACCTTAAAAGAAGCGAGACACTCAAGAAGCAACAAACACTTGGAGAAATGAGTTTGGAGGATTTCTTAGTAAAAGCTGGAGTAGTACAACAATCATCAGCATTGCCATTCAAGAATCATAATGGCAATGTTTCAGGTAATATGAGACCATTGAATATTGCAAGTTGTTATGGTTTGAGGCCTTCAATGGGAATGGGATTTTCTACTCAATGTGTGTCTAGAAATGGTCTTGCAACATACCAAATGTTGTCACTGAATAACAACTTAGGAGTGAAGGAATGTGCTGTGGAGAAATGTCAAAGCTTGACTGAATCAAGTGGGTGTAGTAATAGGAAGAGGATAATTGAGGGTCCTCCTGATGTTGTAGTGGAGCGAAGGCAACGTAGAATGTTGAAAAATCGTGAATCGGCTGCTCGTTCTCGAGCAAGAAGACAGGCATATACATTAGAGCTAGAGGCAGAGCTGAATCTGCTAAAAGAAGAGAATGAAAATCTAAAACAAGTTCTGGCTGAAGCTGAGACCAAGAGGAAACAAGAGGTAACTTTTGCAACGAAAGCATTCAACAAAGGCTCAAAAGGGCGCACAAAAATTAAGAGCAATGAGAAGGCCTATAAGCACAACCTGGTGAACAAGCCTCAATGA
Protein sequence

>MsaT010184.1

MALQQLDQEVPLQEQAEATFSQPSKQNSILSLTLDEFQCKSGKSFGSLNMDEFLASIWSSNDEATTQTQNAKNVATTEHTVSQQLGNSFSVPPPIWKKTVDEVWSEIHKNQPQFKETNNLKRSETLKKQQTLGEMSLEDFLVKAGVVQQSSALPFKNHNGNVSGNMRPLNIASCYGLRPSMGMGFSTQCVSRNGLATYQMLSLNNNLGVKECAVEKCQSLTESSGCSNRKRIIEGPPDVVVERRQRRMLKNRESAARSRARRQAYTLELEAELNLLKEENENLKQVLAEAETKRKQEVTFATKAFNKGSKGRTKIKSNEKAYKHNLVNKPQ*