AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010185

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010185 MtrunA17_Chr2g0321411 83.103 290 29 3 1 272 1 288 1.20e-170 473
MsaG010185 MtrunA17_Chr2g0321381 63.542 288 56 7 1 240 1 287 3.60e-108 315
MsaG010185 MtrunA17_Chr3g0080341 32.372 312 118 12 19 257 29 320 2.17e-26 105
MsaG010185 MtrunA17_Chr8g0355121 35.338 266 114 12 18 237 32 285 5.80e-26 103
MsaG010185 MtrunA17_Chr7g0254991 33.216 283 112 10 19 238 25 293 2.97e-24 99.4
MsaG010185 MtrunA17_Chr7g0266211 42.222 135 61 3 18 135 47 181 1.41e-23 99.0
MsaG010185 MtrunA17_Chr7g0266211 57.732 97 38 1 172 265 323 419 1.16e-20 90.9
MsaG010185 MtrunA17_Chr6g0460301 32.609 276 122 9 19 237 25 293 7.93e-18 81.6
MsaG010185 MtrunA17_Chr5g0440841 31.081 222 135 5 17 237 37 241 2.30e-17 79.7
MsaG010185 MtrunA17_Chr3g0133971 52.564 78 30 1 160 237 325 395 3.24e-15 75.1
MsaG010185 MtrunA17_Chr3g0133971 37.600 125 57 6 18 134 35 146 7.31e-14 70.9
MsaG010185 MtrunA17_Chr4g0044771 45.098 102 44 3 163 263 281 371 5.31e-13 68.2
MsaG010185 MtrunA17_Chr4g0051101 35.556 135 63 4 117 251 77 187 1.55e-12 64.7
MsaG010185 MtrunA17_Chr1g0201751 28.641 206 109 5 68 253 34 221 3.88e-11 61.6
MsaG010185 MtrunA17_Chr5g0405951 39.516 124 53 3 141 249 120 236 5.15e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010185 AT3G44460.1 42.308 286 118 10 18 265 43 319 3.43e-49 166
MsaG010185 AT3G56850.1 34.615 260 116 11 18 237 24 269 6.02e-28 109
MsaG010185 AT2G36270.3 56.436 101 43 1 151 250 312 412 1.69e-27 110
MsaG010185 AT2G36270.3 45.333 75 28 2 71 132 145 219 1.44e-11 64.7
MsaG010185 AT2G36270.1 55.340 103 45 1 151 252 312 414 2.06e-27 110
MsaG010185 AT2G36270.1 32.203 177 56 6 18 132 45 219 1.40e-15 76.6
MsaG010185 AT2G36270.2 55.340 103 45 1 151 252 312 414 2.06e-27 110
MsaG010185 AT2G36270.2 32.203 177 56 6 18 132 45 219 1.40e-15 76.6
MsaG010185 AT2G41070.5 32.287 223 116 4 25 238 39 235 1.98e-25 101
MsaG010185 AT2G41070.3 32.287 223 116 4 25 238 39 235 4.38e-25 100
MsaG010185 AT2G41070.1 32.287 223 116 4 25 238 39 235 4.38e-25 100
MsaG010185 AT2G41070.2 32.287 223 116 4 25 238 39 235 4.38e-25 100
MsaG010185 AT2G41070.4 32.287 223 116 4 25 238 48 244 5.00e-25 100
MsaG010185 AT5G44080.1 29.787 188 99 5 81 252 120 290 1.43e-15 75.5
MsaG010185 AT1G45249.1 48.718 78 32 1 160 237 311 380 8.95e-15 73.9
MsaG010185 AT1G45249.1 32.576 132 75 4 18 137 29 158 3.71e-11 63.2
MsaG010185 AT1G45249.7 48.718 78 32 1 160 237 326 395 1.06e-14 73.9
MsaG010185 AT1G45249.7 32.576 132 75 4 18 137 44 173 3.54e-11 63.5
MsaG010185 AT1G45249.6 48.718 78 32 1 160 237 326 395 1.06e-14 73.9
MsaG010185 AT1G45249.6 32.576 132 75 4 18 137 44 173 3.54e-11 63.5
MsaG010185 AT1G03970.1 30.168 179 101 5 81 252 85 246 3.85e-14 70.9
MsaG010185 AT4G34000.1 46.667 90 38 2 163 252 352 431 7.40e-14 71.6
MsaG010185 AT4G34000.4 46.667 90 38 2 163 252 352 431 7.40e-14 71.6
MsaG010185 AT4G34000.2 46.667 90 38 2 163 252 352 431 7.40e-14 71.6
MsaG010185 AT3G19290.1 59.016 61 25 0 190 250 348 408 1.79e-13 70.1
MsaG010185 AT3G19290.5 59.016 61 25 0 190 250 348 408 1.79e-13 70.1
MsaG010185 AT3G19290.4 56.452 62 27 0 190 251 348 409 1.83e-13 70.1
MsaG010185 AT3G19290.3 51.316 76 35 1 190 265 348 421 3.64e-13 69.3
MsaG010185 AT1G49720.1 33.333 147 75 5 18 148 31 170 4.28e-13 68.9
MsaG010185 AT1G49720.1 55.738 61 27 0 190 250 308 368 7.60e-12 65.5
MsaG010185 AT1G49720.3 33.333 147 75 5 18 148 31 170 4.28e-13 68.9
MsaG010185 AT1G49720.3 55.738 61 27 0 190 250 308 368 7.60e-12 65.5
MsaG010185 AT1G49720.2 33.333 147 75 5 18 148 31 170 4.94e-13 68.9
MsaG010185 AT1G49720.2 53.226 62 29 0 190 251 308 369 9.67e-12 65.1
MsaG010185 AT2G17770.2 32.143 196 107 7 84 257 2 193 2.42e-12 64.7
MsaG010185 AT4G34000.3 64.583 48 17 0 190 237 369 416 3.43e-12 66.6
MsaG010185 AT1G45249.3 42.697 89 32 2 160 237 311 391 6.88e-12 65.5
MsaG010185 AT1G45249.3 32.576 132 75 4 18 137 29 158 4.68e-11 63.2
MsaG010185 AT4G35900.1 52.941 68 27 1 195 257 216 283 6.52e-11 62.0
MsaG010185 AT1G45249.10 32.576 132 75 4 18 137 29 158 9.55e-11 62.0
MsaG010185 AT1G45249.5 32.576 132 75 4 18 137 29 158 9.55e-11 62.0

Find 45 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATCAATTATCCTCTTTC+TGG 0.164582 2:-69777876 None:intergenic
ATGAGTTCCTAGCCAGTATT+TGG 0.254341 2:+69777435 MsaT010185.1:CDS
TAAAATGCTGAAACAATTTC+TGG 0.256037 2:+69778402 MsaT010185.1:CDS
CTTGTCATTTGGTATGTTGC+AGG 0.294849 2:-69777827 None:intergenic
TTTCGCTCCAATAGAACTTC+AGG 0.322405 2:-69777902 None:intergenic
CCACTGAATATTGCAAGTTC+TGG 0.324558 2:+69777788 MsaT010185.1:CDS
ACTCAGCTCCACTTGTCATT+TGG 0.334133 2:-69777838 None:intergenic
AGAAAACTTCGGATGAAGTT+TGG 0.353717 2:+69777576 MsaT010185.1:CDS
CAAAGTTCTTGAAATCATCT+AGG 0.390515 2:-69777397 None:intergenic
GCAATCAGCAAAGGTTCAAA+AGG 0.420453 2:+69778652 MsaT010185.1:CDS
GACTGAATCACCAAGTTGTT+GGG 0.423167 2:-69777531 None:intergenic
ATCACTGGAACTCCAAATAC+TGG 0.430426 2:-69777447 None:intergenic
TTTCTTCTTTGAATTGTGGT+TGG 0.440685 2:-69777616 None:intergenic
GAGGATTTCTTGGTAAAAGC+TGG 0.453104 2:+69777698 MsaT010185.1:CDS
GTCATTGAGGCTTATTCACC+AGG 0.470334 2:-69778702 None:intergenic
AGACTGAATCACCAAGTTGT+TGG 0.471123 2:-69777532 None:intergenic
ATCACCAAGTTGTTGGGAAC+TGG 0.493001 2:-69777525 None:intergenic
CACACCAGTTCCCAACAACT+TGG 0.498257 2:+69777521 MsaT010185.1:CDS
TCCTCAAAAGTCATTTCTCC+AGG 0.507331 2:-69777680 None:intergenic
CAATCAGCAAAGGTTCAAAA+GGG 0.509524 2:+69778653 MsaT010185.1:CDS
TTCGCTCCAATAGAACTTCA+GGG 0.513014 2:-69777901 None:intergenic
TAGGAACTCATCCATGTTGA+GGG 0.517404 2:-69777423 None:intergenic
TTGTGTAGTAGCTTCATCAC+TGG 0.520796 2:-69777462 None:intergenic
ACGTTCTCGAGCAAGACGTC+AGG 0.524988 2:+69777961 MsaT010185.1:CDS
ACCAGAAAGAGGATAATTGA+TGG 0.532983 2:+69777875 MsaT010185.1:CDS
TCCAGTTTGCAAGAAAACTT+CGG 0.535572 2:+69777565 MsaT010185.1:CDS
AAAGTTCTTGAAATCATCTA+GGG 0.543413 2:-69777396 None:intergenic
GCAAAGAAAGCAATCAGCAA+AGG 0.559335 2:+69778643 MsaT010185.1:CDS
TTGTCGTTTCAGAATCATAG+TGG 0.559475 2:+69777743 MsaT010185.1:CDS
CGCTCCAATAGAACTTCAGG+GGG 0.565451 2:-69777899 None:intergenic
CTAGGAACTCATCCATGTTG+AGG 0.566833 2:-69777424 None:intergenic
CTCAAGAAGCAAGAAACACC+TGG 0.571531 2:+69777662 MsaT010185.1:CDS
AGGCTGAAGCTGAGAACAAG+AGG 0.580689 2:+69778521 MsaT010185.1:intron
TGGAACTCCAAATACTGGCT+AGG 0.584202 2:-69777442 None:intergenic
TCTACAACAATTAGATCAAG+AGG 0.584508 2:+69777343 MsaT010185.1:CDS
AATCAAGCTCAAGCACAACC+TGG 0.585556 2:+69778684 MsaT010185.1:CDS
GTGGAGCTGCTACCAGAAAG+AGG 0.587980 2:+69777864 MsaT010185.1:CDS
GGCTTATACAATAGAGCTAG+AGG 0.593351 2:+69778351 MsaT010185.1:intron
GAACTTTGACTCCCTCAACA+TGG 0.606896 2:+69777412 MsaT010185.1:CDS
GCAACATACCAAATGACAAG+TGG 0.610150 2:+69777830 MsaT010185.1:CDS
ACAAGTGGAGCTGAGTCAAG+TGG 0.615825 2:+69777845 MsaT010185.1:CDS
CCAGAACTTGCAATATTCAG+TGG 0.629052 2:-69777788 None:intergenic
TCGCTCCAATAGAACTTCAG+GGG 0.629189 2:-69777900 None:intergenic
TGAGAACAAGAGGAAACAAG+AGG 0.643173 2:+69778531 MsaT010185.1:CDS
AATCAGCAAAGGTTCAAAAG+GGG 0.678708 2:+69778654 MsaT010185.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 69777338 69778709 69777338 ID=MsaG010185
Chr2 mRNA 69777338 69778709 69777338 ID=MsaT010185.1;Parent=MsaG010185
Chr2 exon 69777338 69777982 69777338 ID=MsaT010185.1.exon1;Parent=MsaT010185.1
Chr2 CDS 69777338 69777982 69777338 ID=cds.MsaT010185.1;Parent=MsaT010185.1
Chr2 exon 69778352 69778423 69778352 ID=MsaT010185.1.exon2;Parent=MsaT010185.1
Chr2 CDS 69778352 69778423 69778352 ID=cds.MsaT010185.1;Parent=MsaT010185.1
Chr2 exon 69778523 69778552 69778523 ID=MsaT010185.1.exon3;Parent=MsaT010185.1
Chr2 CDS 69778523 69778552 69778523 ID=cds.MsaT010185.1;Parent=MsaT010185.1
Chr2 exon 69778638 69778709 69778638 ID=MsaT010185.1.exon4;Parent=MsaT010185.1
Chr2 CDS 69778638 69778709 69778638 ID=cds.MsaT010185.1;Parent=MsaT010185.1
Gene Sequence

>MsaT010185.1

ATGGCTCTACAACAATTAGATCAAGAGGATTCTCATCCGACCAATCAAAACGCACTTACCCTAGATGATTTCAAGAACTTTGACTCCCTCAACATGGATGAGTTCCTAGCCAGTATTTGGAGTTCCAGTGATGAAGCTACTACACAAACTCACAACAATATTGAAAGTGTTGTAACAACAGAACACACCAGTTCCCAACAACTTGGTGATTCAGTCTCTGTCCCTCCTCCAGTTTGCAAGAAAACTTCGGATGAAGTTTGGTCCGAGATTCATAAAAACCAACCACAATTCAAAGAAGAAAATAACCTTAAAAGAAATGAGACACTCAAGAAGCAAGAAACACCTGGAGAAATGACTTTTGAGGATTTCTTGGTAAAAGCTGGAGTAGTACAACAATCATCATCATTGTCGTTTCAGAATCATAGTGGCAATGTTTCAAATAACATGAGACCACTGAATATTGCAAGTTCTGGTTTGACTAGCAATAGTCCTGCAACATACCAAATGACAAGTGGAGCTGAGTCAAGTGGAGCTGCTACCAGAAAGAGGATAATTGATGGTCCCCCTGAAGTTCTATTGGAGCGAAAACAACGCAGAATGATGAAAAATCGAGAATCGGCTGCACGTTCTCGAGCAAGACGTCAGGCTTATACAATAGAGCTAGAGGCTGAGCTGAATCTGCTACAAGAAGAGAATAAAATGCTGAAACAATTTCTGGCTGAAGCTGAGAACAAGAGGAAACAAGAGCTTTTGCAAAGAAAGCAATCAGCAAAGGTTCAAAAGGGGACAGAAAAATCAAGCTCAAGCACAACCTGGTGA
Protein sequence

>MsaT010185.1

MALQQLDQEDSHPTNQNALTLDDFKNFDSLNMDEFLASIWSSSDEATTQTHNNIESVVTTEHTSSQQLGDSVSVPPPVCKKTSDEVWSEIHKNQPQFKEENNLKRNETLKKQETPGEMTFEDFLVKAGVVQQSSSLSFQNHSGNVSNNMRPLNIASSGLTSNSPATYQMTSGAESSGAATRKRIIDGPPEVLLERKQRRMMKNRESAARSRARRQAYTIELEAELNLLQEENKMLKQFLAEAENKRKQELLQRKQSAKVQKGTEKSSSSTTW*