AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010187

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010187 MtrunA17_Chr2g0321431 94.639 429 9 3 6 420 69 497 0.0 826
MsaG010187 MtrunA17_Chr4g0026401 64.091 440 113 8 7 420 69 489 0.0 548
MsaG010187 MtrunA17_Chr8g0353501 61.111 288 90 3 127 392 179 466 3.32e-119 355
MsaG010187 MtrunA17_Chr1g0174701 47.458 413 151 9 11 392 129 506 2.93e-117 351
MsaG010187 MtrunA17_Chr7g0256211 57.947 302 104 4 113 392 152 452 4.95e-117 349
MsaG010187 MtrunA17_Chr7g0258321 53.312 317 116 4 108 392 147 463 2.34e-115 345
MsaG010187 MtrunA17_Chr3g0124601 54.110 292 110 5 122 392 73 361 1.14e-102 309
MsaG010187 MtrunA17_Chr1g0210151 45.955 309 140 3 107 392 36 340 3.22e-99 299
MsaG010187 MtrunA17_Chr5g0422771 53.125 288 113 3 127 392 177 464 9.74e-98 300
MsaG010187 MtrunA17_Chr5g0424861 47.436 312 140 6 101 389 37 347 8.70e-87 268
MsaG010187 MtrunA17_Chr3g0115371 48.432 287 127 3 129 394 65 351 1.60e-84 262
MsaG010187 MtrunA17_Chr8g0382861 48.772 285 122 5 129 392 75 356 1.72e-82 257
MsaG010187 MtrunA17_Chr3g0093451 46.237 186 85 4 107 280 27 209 9.31e-56 183
MsaG010187 MtrunA17_Chr5g0424851 42.534 221 112 2 184 389 2 222 7.87e-52 179
MsaG010187 MtrunA17_Chr5g0424851 40.796 201 105 2 194 380 226 426 1.03e-46 166
MsaG010187 MtrunA17_Chr1g0196951 32.143 196 104 6 205 385 59 240 1.06e-20 90.5
MsaG010187 MtrunA17_Chr8g0384821 25.214 234 126 5 196 380 15 248 4.23e-13 69.3
MsaG010187 MtrunA17_Chr7g0242651 23.944 213 138 3 194 382 7 219 1.38e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010187 AT1G08320.1 59.811 423 138 6 13 420 76 481 3.88e-175 498
MsaG010187 AT1G08320.4 59.811 423 138 6 13 420 76 481 3.88e-175 498
MsaG010187 AT1G08320.3 59.811 423 138 6 13 420 76 481 3.88e-175 498
MsaG010187 AT1G08320.2 63.483 356 116 2 77 420 4 357 1.72e-166 471
MsaG010187 AT1G08320.5 63.483 356 116 2 77 420 4 357 1.72e-166 471
MsaG010187 AT5G06839.3 48.449 419 147 10 12 393 73 459 8.01e-116 346
MsaG010187 AT3G12250.6 53.846 325 125 4 89 392 10 330 9.34e-115 339
MsaG010187 AT3G12250.2 53.846 325 125 4 89 392 10 330 9.34e-115 339
MsaG010187 AT3G12250.7 53.846 325 125 4 89 392 10 330 9.34e-115 339
MsaG010187 AT3G12250.1 53.846 325 125 4 89 392 10 330 9.34e-115 339
MsaG010187 AT5G06839.1 56.646 316 101 5 114 393 101 416 2.57e-114 341
MsaG010187 AT3G12250.5 56.667 300 106 4 117 392 4 303 2.06e-113 335
MsaG010187 AT3G12250.3 56.667 300 106 4 117 392 25 324 3.59e-113 335
MsaG010187 AT5G06839.2 56.467 317 101 6 114 393 101 417 4.32e-113 338
MsaG010187 AT5G06950.2 56.000 300 108 4 117 392 31 330 4.89e-113 335
MsaG010187 AT5G06950.4 56.000 300 108 4 117 392 31 330 4.89e-113 335
MsaG010187 AT5G06950.1 56.000 300 108 4 117 392 31 330 4.89e-113 335
MsaG010187 AT5G06950.5 56.000 300 108 4 117 392 31 330 4.89e-113 335
MsaG010187 AT5G06950.3 56.000 300 108 4 117 392 31 330 4.89e-113 335
MsaG010187 AT3G12250.4 55.128 312 115 4 102 392 48 355 6.05e-112 333
MsaG010187 AT5G06960.2 57.639 288 101 3 126 392 43 330 1.89e-110 328
MsaG010187 AT5G06960.1 57.639 288 101 3 126 392 43 330 1.89e-110 328
MsaG010187 AT5G06960.3 57.639 288 101 3 126 392 23 310 2.90e-110 327
MsaG010187 AT1G77920.1 48.225 338 132 6 70 392 57 366 2.53e-98 298
MsaG010187 AT1G22070.1 54.325 289 111 3 126 393 95 383 3.19e-98 299
MsaG010187 AT5G10030.5 52.203 295 116 5 119 392 72 362 3.25e-94 288
MsaG010187 AT5G10030.3 52.203 295 116 5 119 392 72 362 3.25e-94 288
MsaG010187 AT5G10030.1 52.203 295 116 5 119 392 72 362 3.25e-94 288
MsaG010187 AT5G10030.4 52.203 295 116 5 119 392 72 362 3.25e-94 288
MsaG010187 AT5G10030.2 52.203 295 116 5 119 392 72 362 3.25e-94 288
MsaG010187 AT1G68640.1 53.061 294 111 5 124 392 161 452 2.62e-93 288
MsaG010187 AT5G65210.6 50.512 293 124 4 119 392 6 296 9.88e-90 274
MsaG010187 AT5G65210.5 50.512 293 124 4 119 392 76 366 1.90e-89 276
MsaG010187 AT5G65210.7 50.512 293 124 4 119 392 76 366 1.90e-89 276
MsaG010187 AT5G65210.3 50.512 293 124 4 119 392 76 366 1.90e-89 276
MsaG010187 AT5G65210.1 50.512 293 124 4 119 392 76 366 1.90e-89 276
MsaG010187 AT5G65210.2 50.512 293 124 4 119 392 76 366 1.90e-89 276
MsaG010187 AT5G65210.4 50.512 293 124 4 119 392 76 366 1.90e-89 276
MsaG010187 AT1G58330.1 25.339 221 138 5 189 382 3 223 5.89e-11 62.4

Find 86 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACGCTCCTTTCTTGTTAT+TGG 0.137107 2:+69797940 MsaT010187.1:CDS
CACTGGAAATGTTCCCTTCT+TGG 0.211576 2:+69792116 MsaT010187.1:CDS
TTCGAGCTCTTAGTTCTCTT+TGG 0.221414 2:+69797980 MsaT010187.1:CDS
AAGCTGAACGTTGCTTCCTT+TGG 0.305878 2:+69795244 MsaT010187.1:CDS
CTTGTTATTGGAGAATATTA+TGG 0.317768 2:+69797952 MsaT010187.1:CDS
GAAAAGTCGTCTAAGGAAAA+AGG 0.321473 2:+69793585 MsaT010187.1:CDS
ATCAGATCATAATAATAATA+TGG 0.322421 2:+69792489 MsaT010187.1:CDS
ACTAGCTGAGCAGCAAATAA+TGG 0.328572 2:+69796597 MsaT010187.1:CDS
AATTGAAAAGGGTATGTTCA+TGG 0.336673 2:+69793858 MsaT010187.1:intron
AGTTTGTTGCCTCAAGTTAT+CGG 0.350327 2:-69797878 None:intergenic
GCAATGTTTGACATGGAATA+TGG 0.352960 2:+69795033 MsaT010187.1:CDS
TCGAGCTCTTAGTTCTCTTT+GGG 0.354428 2:+69797981 MsaT010187.1:CDS
CTAGCTGAGCAGCAAATAAT+GGG 0.360569 2:+69796598 MsaT010187.1:CDS
TGCTGGAGGACCCTTGTTGA+TGG 0.364816 2:+69796711 MsaT010187.1:intron
TAGATTAAAGCTTACACAAT+TGG 0.368639 2:+69793724 MsaT010187.1:CDS
CAGCTAGTGGCTCCAATTGT+TGG 0.369121 2:-69796582 None:intergenic
AAGGGTGATTCTTGCTCAAA+AGG 0.370182 2:-69792442 None:intergenic
TTACTACTGTTGAGGTTTGA+TGG 0.373913 2:-69792150 None:intergenic
CCTGAGAGCGTGCTCTTTGA+AGG 0.382110 2:-69793754 None:intergenic
TTTGCATGTCTGTGGTTGTT+TGG 0.382914 2:-69798323 None:intergenic
TTCCAGTGTAGCAGCAGGTC+TGG 0.387739 2:-69792101 None:intergenic
TGAGACGTTTAGCACAAAAC+AGG 0.401597 2:+69793551 MsaT010187.1:CDS
TTAGATGTTGATCCAACAAT+TGG 0.405853 2:+69796570 MsaT010187.1:intron
ATGGATTGGAGTGGAGGTGT+TGG 0.411382 2:+69793877 MsaT010187.1:CDS
GTGTGAGTAATCGACATAAT+TGG 0.411816 2:-69797906 None:intergenic
CGTTGCTTCCTTTGGATTGG+TGG 0.418885 2:+69795252 MsaT010187.1:CDS
TTCTTAACTCCGTTAATAAA+CGG 0.419059 2:-69795077 None:intergenic
CTGCAAGGAAAAGTCGTCTA+AGG 0.422741 2:+69793578 MsaT010187.1:CDS
GGCAAGCTTGCCAATCTTGA+AGG 0.422961 2:+69796759 MsaT010187.1:CDS
AGAAGACAACCGTTTATTAA+CGG 0.423518 2:+69795068 MsaT010187.1:CDS
ATCCAGACCTGCTGCTACAC+TGG 0.426810 2:+69792099 MsaT010187.1:CDS
CAGGAAGGTGGTAACAAATC+AGG 0.441251 2:+69792376 MsaT010187.1:intron
GAACGTTGCTTCCTTTGGAT+TGG 0.451843 2:+69795249 MsaT010187.1:CDS
GACGTGTTTCATCTTATCAA+TGG 0.455217 2:+69795207 MsaT010187.1:CDS
CAGCAAATAATGGGGATGTA+TGG 0.463856 2:+69796607 MsaT010187.1:CDS
GCGAAGAAAACCTTCAAGAT+TGG 0.465454 2:-69796769 None:intergenic
CAACAAATGGTTGTTGCCAT+AGG 0.467436 2:+69796738 MsaT010187.1:CDS
TAGACCCTCTGAAATTATCA+TGG 0.469132 2:+69795278 MsaT010187.1:CDS
AAGATTGGCAAGCTTGCCTA+TGG 0.469646 2:-69796754 None:intergenic
AAAAGGGTATGTTCATGGAT+TGG 0.472621 2:+69793863 MsaT010187.1:intron
TGGAATCTCATTGGCCAAGA+AGG 0.473970 2:-69792130 None:intergenic
AGGGTGATTCTTGCTCAAAA+GGG 0.490521 2:-69792441 None:intergenic
ATGTTCATGGATTGGAGTGG+AGG 0.491384 2:+69793871 MsaT010187.1:CDS
TGTTGATGGTGTTCAACAAA+TGG 0.493901 2:+69796725 MsaT010187.1:CDS
CCTTCAAAGAGCACGCTCTC+AGG 0.494782 2:+69793754 MsaT010187.1:CDS
GAGGTTTGATGGAATCTCAT+TGG 0.494784 2:-69792139 None:intergenic
GATTGGAGTGGAGGTGTTGG+AGG 0.501212 2:+69793880 MsaT010187.1:CDS
ACTAACCATGATAATTTCAG+AGG 0.514912 2:-69795283 None:intergenic
ATAAACACCTTTGCATCAAG+TGG 0.519298 2:-69793437 None:intergenic
ATCAGCAGCAGCCAAAGAAA+AGG 0.524596 2:+69792606 MsaT010187.1:CDS
ATTATTTGCTGCTCAGCTAG+TGG 0.524704 2:-69796595 None:intergenic
GATCAAATGTTTAGACTCAA+AGG 0.541535 2:+69795168 MsaT010187.1:CDS
AACAACCACAGACATGCAAA+TGG 0.543202 2:+69798326 MsaT010187.1:CDS
CACAAAACAGGGAGGCTGCA+AGG 0.543811 2:+69793563 MsaT010187.1:CDS
ATATTCTCCAATAACAAGAA+AGG 0.546712 2:-69797947 None:intergenic
AACATTTCCAGTGTAGCAGC+AGG 0.549096 2:-69792106 None:intergenic
GTTGGAGGAAATATAAGCTC+TGG 0.554357 2:+69793895 MsaT010187.1:CDS
TTATTAACGGAGTTAAGAAA+CGG 0.562286 2:+69795081 MsaT010187.1:CDS
GGAATCTCATTGGCCAAGAA+GGG 0.563800 2:-69792129 None:intergenic
AGATAAGCCACTTGATGCAA+AGG 0.567470 2:+69793430 MsaT010187.1:CDS
GTCTAAAACCACCAATCCAA+AGG 0.567699 2:-69795260 None:intergenic
AGAGAGTATTACTACTGTTG+AGG 0.572603 2:-69792158 None:intergenic
TTTGTGAAATGATTCTGAGA+AGG 0.574512 2:-69798354 None:intergenic
GCAGAGGAAGCTCTCTCACA+AGG 0.575222 2:+69796652 MsaT010187.1:CDS
AATTGAAGGCCGATAACTTG+AGG 0.576952 2:+69797869 MsaT010187.1:intron
TTGACATGGAATATGGAAGA+TGG 0.583833 2:+69795040 MsaT010187.1:CDS
AGATAATGAAATGAGAGTAA+TGG 0.594040 2:+69795122 MsaT010187.1:CDS
TATTTGGAAATACAGGAAGG+TGG 0.595349 2:+69792364 MsaT010187.1:intron
TATTTGATTCTGTGATGAAG+AGG 0.598778 2:-69792584 None:intergenic
GAAATGAGAGTAATGGTGGA+TGG 0.599016 2:+69795129 MsaT010187.1:CDS
TTTGTTGAACACCATCAACA+AGG 0.603469 2:-69796722 None:intergenic
TTGAACCATTTGCATGTCTG+TGG 0.607233 2:-69798331 None:intergenic
ATAGATTTAACTTACTCTCG+TGG 0.607609 2:-69798012 None:intergenic
TTCATCTTATCAATGGCATG+TGG 0.610713 2:+69795214 MsaT010187.1:CDS
TAATGAAATGAGAGTAATGG+TGG 0.614982 2:+69795125 MsaT010187.1:CDS
GCATTCTTCGCAGCAAGCAG+AGG 0.615108 2:+69796636 MsaT010187.1:CDS
GTTAAGAAACGGATTGCAAG+CGG 0.631491 2:+69795092 MsaT010187.1:CDS
GAGACGTTTAGCACAAAACA+GGG 0.648005 2:+69793552 MsaT010187.1:CDS
AGGAGGTGCAATGTTTGACA+TGG 0.651643 2:+69795026 MsaT010187.1:intron
TTGTTGAACACCATCAACAA+GGG 0.655686 2:-69796721 None:intergenic
CTAACCATGATAATTTCAGA+GGG 0.659541 2:-69795282 None:intergenic
GAAGGTGGTAACAAATCAGG+AGG 0.695839 2:+69792379 MsaT010187.1:CDS
TAATGGGGATGTATGGACTG+AGG 0.696038 2:+69796614 MsaT010187.1:CDS
GGTATGTTCATGGATTGGAG+TGG 0.698181 2:+69793868 MsaT010187.1:CDS
TAGCTGAGCAGCAAATAATG+GGG 0.701228 2:+69796599 MsaT010187.1:CDS
ACGTTTAGCACAAAACAGGG+AGG 0.779708 2:+69793555 MsaT010187.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 69791289 69798383 69791289 ID=MsaG010187
Chr2 mRNA 69791289 69798383 69791289 ID=MsaT010187.1;Parent=MsaG010187
Chr2 exon 69791289 69791304 69791289 ID=MsaT010187.1.exon1;Parent=MsaT010187.1
Chr2 CDS 69791289 69791304 69791289 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69792089 69792165 69792089 ID=MsaT010187.1.exon2;Parent=MsaT010187.1
Chr2 CDS 69792089 69792165 69792089 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69792379 69792627 69792379 ID=MsaT010187.1.exon3;Parent=MsaT010187.1
Chr2 CDS 69792379 69792627 69792379 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69793407 69793451 69793407 ID=MsaT010187.1.exon4;Parent=MsaT010187.1
Chr2 CDS 69793407 69793451 69793407 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69793547 69793606 69793547 ID=MsaT010187.1.exon5;Parent=MsaT010187.1
Chr2 CDS 69793547 69793606 69793547 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69793698 69793775 69793698 ID=MsaT010187.1.exon6;Parent=MsaT010187.1
Chr2 CDS 69793698 69793775 69793698 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69793868 69793916 69793868 ID=MsaT010187.1.exon7;Parent=MsaT010187.1
Chr2 CDS 69793868 69793916 69793868 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69795028 69795299 69795028 ID=MsaT010187.1.exon8;Parent=MsaT010187.1
Chr2 CDS 69795028 69795299 69795028 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69796574 69796683 69796574 ID=MsaT010187.1.exon9;Parent=MsaT010187.1
Chr2 CDS 69796574 69796683 69796574 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69796719 69796794 69796719 ID=MsaT010187.1.exon10;Parent=MsaT010187.1
Chr2 CDS 69796719 69796794 69796719 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69797877 69798019 69797877 ID=MsaT010187.1.exon11;Parent=MsaT010187.1
Chr2 CDS 69797877 69798019 69797877 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Chr2 exon 69798296 69798383 69798296 ID=MsaT010187.1.exon12;Parent=MsaT010187.1
Chr2 CDS 69798296 69798383 69798296 ID=cds.MsaT010187.1;Parent=MsaT010187.1
Gene Sequence

>MsaT010187.1

ATGACATCATATATAACTTTTTTCACATCCAGACCTGCTGCTACACTGGAAATGTTCCCTTCTTGGCCAATGAGATTCCATCAAACCTCAACAGAAGGTGGTAACAAATCAGGAGGTGAAAGCAGTGATTCAGCACTATCAAGCAAAAATGAGAACCCTTTTGAGCAAGAATCACCCTTAAGTAAAAAAGCATCTTCTTTTTCATCAGATCATAATAATAATATGGATCAGCAGAATCTTCAGCTACAACAACAGAAGATAATAATATCAAATGATGCTTCTGCTGCTATAAGAACAGCCTCTTCATCACAGAATCAAATATCAGCAGCAGCCAAAGAAAAGAAAAAAGGAGCTGGTTCAACATCAGATAAGCCACTTGATGCAAAGACATTGAGACGTTTAGCACAAAACAGGGAGGCTGCAAGGAAAAGTCGTCTAAGGAAAAAGGCTTATGTGCAGCAACTTGAGTCAAGTAGATTAAAGCTTACACAATTGGAACAAGACCTTCAAAGAGCACGCTCTCAGGGTATGTTCATGGATTGGAGTGGAGGTGTTGGAGGAAATATAAGCTCTGGAGGTGCAATGTTTGACATGGAATATGGAAGATGGTTAGAAGAAGACAACCGTTTATTAACGGAGTTAAGAAACGGATTGCAAGCGGCGTTAACAGATAATGAAATGAGAGTAATGGTGGATGGATATTTGTGTCATTATGATCAAATGTTTAGACTCAAAGGTGTCACTGCTAAATCAGACGTGTTTCATCTTATCAATGGCATGTGGACTTCTCAAGCTGAACGTTGCTTCCTTTGGATTGGTGGTTTTAGACCCTCTGAAATTATCATGATGTTGATCCAACAATTGGAGCCACTAGCTGAGCAGCAAATAATGGGGATGTATGGACTGAGGCATTCTTCGCAGCAAGCAGAGGAAGCTCTCTCACAAGGACTTGACCAGACCCTTGTTGATGGTGTTCAACAAATGGTTGTTGCCATAGGCAAGCTTGCCAATCTTGAAGGTTTTCTTCGCCAGGCCGATAACTTGAGGCAACAAACTCTTCACCAATTATGTCGATTACTCACACTTCGTCAAGCAGCACGCTCCTTTCTTGTTATTGGAGAATATTATGGACGACTTCGAGCTCTTAGTTCTCTTTGGGCATCAAGACCACGAGAGAACATGATCAGTGATGATAACTCATGCCAAACAACCACAGACATGCAAATGGTTCAACCTTCTCAGAATCATTTCACAAATTTCTGA
Protein sequence

>MsaT010187.1

MTSYITFFTSRPAATLEMFPSWPMRFHQTSTEGGNKSGGESSDSALSSKNENPFEQESPLSKKASSFSSDHNNNMDQQNLQLQQQKIIISNDASAAIRTASSSQNQISAAAKEKKKGAGSTSDKPLDAKTLRRLAQNREAARKSRLRKKAYVQQLESSRLKLTQLEQDLQRARSQGMFMDWSGGVGGNISSGGAMFDMEYGRWLEEDNRLLTELRNGLQAALTDNEMRVMVDGYLCHYDQMFRLKGVTAKSDVFHLINGMWTSQAERCFLWIGGFRPSEIIMMLIQQLEPLAEQQIMGMYGLRHSSQQAEEALSQGLDQTLVDGVQQMVVAIGKLANLEGFLRQADNLRQQTLHQLCRLLTLRQAARSFLVIGEYYGRLRALSSLWASRPRENMISDDNSCQTTTDMQMVQPSQNHFTNF*