Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| MsaG010258 | TF | C2C2-YABBY |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCCCTGTGACTGATATC+TGG | 0.320339 | 2:+70702230 | None:intergenic |
| GGAGGAAATACAAAGAATTA+AGG | 0.364362 | 2:-70702261 | MsaT010258.1:CDS |
| CTTGTTGAGGAGGAAAAGAT+TGG | 0.403943 | 2:+70705262 | None:intergenic |
| AAGGAATATTGTCTCTTCTC+TGG | 0.428614 | 2:+70703133 | None:intergenic |
| AACCTTGGGATGGCTCTTCA+AGG | 0.464363 | 2:+70700594 | None:intergenic |
| TTGTTGAGGAGGAAAAGATT+GGG | 0.470988 | 2:+70705263 | None:intergenic |
| CTTCACCTCCTCAAAACCTT+GGG | 0.475307 | 2:+70700580 | None:intergenic |
| CCTTCACCTCCTCAAAACCT+TGG | 0.483952 | 2:+70700579 | None:intergenic |
| GCTCTCAGTCGTGTCGATGA+AGG | 0.513865 | 2:-70700626 | MsaT010258.1:CDS |
| GAAGGAGAAATCTGAAAAGC+AGG | 0.534340 | 2:-70700561 | MsaT010258.1:CDS |
| AATTGAAGTAGCAGGAGAGT+GGG | 0.534401 | 2:-70700534 | MsaT010258.1:CDS |
| AAATTGAAGTAGCAGGAGAG+TGG | 0.545709 | 2:-70700535 | MsaT010258.1:CDS |
| ATGAAACTGATTTATACAGA+AGG | 0.557434 | 2:+70703114 | None:intergenic |
| GTAATCCAGATATCAGTCAC+AGG | 0.560520 | 2:-70702235 | MsaT010258.1:CDS |
| GAAATGAAATTATACTCTGA+GGG | 0.560932 | 2:+70705235 | None:intergenic |
| CTCTGAGGGTGATCTTGTTG+AGG | 0.561955 | 2:+70705249 | None:intergenic |
| CAGGTGGAAATTGAAGTAGC+AGG | 0.574537 | 2:-70700542 | MsaT010258.1:CDS |
| ACCTCCTCAAAACCTTGGGA+TGG | 0.582517 | 2:+70700584 | None:intergenic |
| TGAGGGTGATCTTGTTGAGG+AGG | 0.589945 | 2:+70705252 | None:intergenic |
| AGCCTTGAAGAGCCATCCCA+AGG | 0.600501 | 2:-70700596 | MsaT010258.1:CDS |
| TGTTGAGGAGGAAAAGATTG+GGG | 0.627464 | 2:+70705264 | None:intergenic |
| GGAGAAATCTGAAAAGCAGG+TGG | 0.639270 | 2:-70700558 | MsaT010258.1:CDS |
| TAATCCAGATATCAGTCACA+GGG | 0.666315 | 2:-70702234 | MsaT010258.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 70700511 | 70705286 | 70700511 | ID=MsaG010258 |
| Chr2 | mRNA | 70700511 | 70705286 | 70700511 | ID=MsaT010258.1;Parent=MsaG010258 |
| Chr2 | exon | 70700511 | 70700648 | 70700511 | ID=MsaT010258.1.exon4;Parent=MsaT010258.1 |
| Chr2 | CDS | 70700511 | 70700648 | 70700511 | ID=cds.MsaT010258.1;Parent=MsaT010258.1 |
| Chr2 | exon | 70702208 | 70702283 | 70702208 | ID=MsaT010258.1.exon3;Parent=MsaT010258.1 |
| Chr2 | CDS | 70702208 | 70702283 | 70702208 | ID=cds.MsaT010258.1;Parent=MsaT010258.1 |
| Chr2 | exon | 70703121 | 70703157 | 70703121 | ID=MsaT010258.1.exon2;Parent=MsaT010258.1 |
| Chr2 | CDS | 70703121 | 70703157 | 70703121 | ID=cds.MsaT010258.1;Parent=MsaT010258.1 |
| Chr2 | exon | 70705250 | 70705286 | 70705250 | ID=MsaT010258.1.exon1;Parent=MsaT010258.1 |
| Chr2 | CDS | 70705250 | 70705286 | 70705250 | ID=cds.MsaT010258.1;Parent=MsaT010258.1 |
| Gene Sequence |
| Protein sequence |
