AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010293

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010293 MtrunA17_Chr2g0322731 98.968 775 3 2 1 775 1 770 0.0 1583
MsaG010293 MtrunA17_Chr4g0024351 84.718 746 94 10 38 775 1 734 0.0 1249
MsaG010293 MtrunA17_Chr8g0350321 55.749 748 279 12 63 773 77 809 0.0 800
MsaG010293 MtrunA17_Chr7g0246631 57.163 705 259 14 98 773 87 777 0.0 786
MsaG010293 MtrunA17_Chr1g0203671 52.439 738 305 11 71 770 60 789 0.0 753
MsaG010293 MtrunA17_Chr6g0481041 49.222 707 317 14 100 771 25 724 0.0 682
MsaG010293 MtrunA17_Chr5g0394111 50.142 702 270 13 83 770 11 646 0.0 647
MsaG010293 MtrunA17_Chr5g0410711 44.490 735 357 14 74 770 57 778 0.0 634
MsaG010293 MtrunA17_Chr2g0331581 45.361 679 329 9 108 773 143 792 0.0 617
MsaG010293 MtrunA17_Chr6g0472471 43.624 745 361 14 71 770 35 765 0.0 601
MsaG010293 MtrunA17_Chr7g0224451 43.067 476 206 11 104 567 16 438 6.11e-122 373
MsaG010293 MtrunA17_Chr2g0292731 36.727 501 298 8 287 774 58 552 1.60e-104 332
MsaG010293 MtrunA17_Chr2g0292741 36.505 515 314 8 268 774 30 539 3.82e-102 325
MsaG010293 MtrunA17_Chr7g0224491 47.335 319 131 8 465 771 1 294 8.56e-84 268
MsaG010293 MtrunA17_Chr7g0224481 41.351 370 160 9 104 462 16 339 1.30e-81 264
MsaG010293 MtrunA17_Chr2g0292761 31.208 596 351 15 183 772 1 543 2.24e-75 254
MsaG010293 MtrunA17_Chr2g0319671 41.860 215 114 2 299 513 65 268 8.69e-50 177
MsaG010293 MtrunA17_Chr1g0205981 43.367 196 99 3 159 350 1 188 8.12e-41 148
MsaG010293 MtrunA17_Chr5g0419821 48.413 126 64 1 100 224 29 154 2.84e-36 134
MsaG010293 MtrunA17_Chr2g0319701 39.207 227 125 6 551 771 5 224 8.25e-36 135
MsaG010293 MtrunA17_Chr2g0319681 36.522 230 137 4 547 771 1 226 2.47e-35 134
MsaG010293 MtrunA17_Chr2g0319691 52.101 119 57 0 405 523 1 119 5.60e-35 130
MsaG010293 MtrunA17_Chr3g0140351 22.691 758 389 27 107 771 20 673 6.08e-27 117
MsaG010293 MtrunA17_Chr7g0224461 47.107 121 46 3 661 770 1 114 3.13e-23 95.5
MsaG010293 MtrunA17_Chr7g0224431 35.484 155 60 3 320 473 6 121 2.43e-16 75.9
MsaG010293 MtrunA17_Chr2g0304191 32.061 131 87 1 301 429 1 131 2.79e-16 76.3
MsaG010293 MtrunA17_Chr4g0028991 22.846 499 280 17 349 771 212 681 2.28e-15 80.5
MsaG010293 MtrunA17_Chr4g0028991 31.405 121 69 3 87 196 8 125 4.34e-13 73.2
MsaG010293 MtrunA17_Chr1g0179581 32.743 113 61 2 75 187 11 108 1.04e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010293 AT1G05230.8 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.4 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.9 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.10 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.7 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.11 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.2 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.6 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.5 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.1 76.819 742 147 7 38 775 1 721 0.0 1140
MsaG010293 AT1G05230.3 76.550 742 147 8 38 775 1 719 0.0 1131
MsaG010293 AT4G04890.2 73.838 753 167 10 38 774 1 739 0.0 1127
MsaG010293 AT4G04890.1 73.838 753 167 10 38 774 1 739 0.0 1127
MsaG010293 AT4G21750.2 72.078 770 175 8 38 775 1 762 0.0 1113
MsaG010293 AT4G21750.1 72.078 770 175 8 38 775 1 762 0.0 1113
MsaG010293 AT4G21750.3 72.078 770 175 8 38 775 1 762 0.0 1113
MsaG010293 AT4G21750.4 72.078 770 175 8 38 775 1 762 0.0 1113
MsaG010293 AT1G05230.12 75.283 619 133 6 38 652 1 603 0.0 943
MsaG010293 AT2G32370.1 53.846 702 288 12 79 767 39 717 0.0 745
MsaG010293 AT3G61150.1 51.268 749 315 8 61 770 66 803 0.0 740
MsaG010293 AT4G00730.1 53.719 726 303 12 63 773 94 801 0.0 734
MsaG010293 AT1G73360.1 47.845 696 338 9 100 775 28 718 0.0 644
MsaG010293 AT1G17920.2 47.076 701 334 12 85 771 5 682 0.0 619
MsaG010293 AT1G17920.1 47.076 701 334 12 85 771 5 682 0.0 619
MsaG010293 AT5G52170.2 45.777 734 327 16 53 770 5 683 0.0 610
MsaG010293 AT5G52170.3 45.913 734 321 18 53 770 5 678 0.0 605
MsaG010293 AT5G52170.1 45.913 734 321 18 53 770 5 678 0.0 605
MsaG010293 AT5G46880.3 45.404 718 343 18 97 775 104 811 0.0 603
MsaG010293 AT5G46880.1 45.404 718 343 18 97 775 104 811 0.0 603
MsaG010293 AT5G46880.2 45.404 718 343 18 97 775 104 811 0.0 603
MsaG010293 AT1G79840.1 42.714 700 337 14 92 773 92 745 0.0 555
MsaG010293 AT1G79840.3 42.714 700 337 14 92 773 34 687 0.0 555
MsaG010293 AT1G79840.6 42.714 700 337 14 92 773 34 687 0.0 555
MsaG010293 AT1G79840.2 42.714 700 337 14 92 773 121 774 0.0 554
MsaG010293 AT4G17710.1 42.258 691 329 17 94 774 77 707 1.46e-171 511
MsaG010293 AT1G79840.4 43.386 567 273 12 92 648 92 620 4.03e-155 466
MsaG010293 AT3G03260.1 38.732 710 392 18 85 771 6 695 1.74e-150 456
MsaG010293 AT4G00730.2 48.954 478 216 8 63 527 94 556 1.01e-145 439
MsaG010293 AT4G25530.1 37.353 680 394 11 95 770 31 682 3.39e-144 439
MsaG010293 AT5G17320.1 34.641 739 412 20 82 773 4 718 3.09e-127 397
MsaG010293 AT1G34650.1 34.233 704 404 15 111 773 23 708 2.46e-116 368
MsaG010293 AT3G03260.2 38.532 545 314 14 85 620 6 538 1.48e-111 350
MsaG010293 AT1G79840.5 42.601 446 208 11 92 527 92 499 1.16e-109 344
MsaG010293 AT5G07260.1 26.364 550 325 17 171 709 11 491 2.11e-43 166
MsaG010293 AT5G07260.2 26.230 549 322 17 171 709 11 486 5.68e-43 164
MsaG010293 AT1G52150.3 21.925 748 409 29 107 771 17 672 3.74e-22 102
MsaG010293 AT1G52150.1 21.925 748 409 29 107 771 17 672 6.59e-22 102
MsaG010293 AT1G52150.2 21.896 749 409 29 107 771 17 673 9.32e-22 101
MsaG010293 AT1G30490.1 22.441 508 295 18 335 771 199 678 1.37e-15 81.6
MsaG010293 AT4G26920.1 21.414 481 258 14 301 765 72 448 7.66e-14 75.1
MsaG010293 AT5G60690.1 25.000 304 192 10 335 621 190 474 3.38e-11 67.4
MsaG010293 AT5G60690.1 27.273 187 92 6 83 238 11 184 4.80e-11 67.0

Find 197 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTACGCTTCCATTTCT+TGG 0.138519 2:+71429118 None:intergenic
AAAATTTCCCCTAGGTTATA+TGG 0.155848 2:-71426938 MsaT010293.1:intron
AACAATAACGTTGGTGTCTT+TGG 0.194570 2:-71429939 MsaT010293.1:CDS
CACTTAAAACCAGCAGGTTT+TGG 0.244194 2:+71427736 None:intergenic
TAGCAATATGTGCCATTTCT+TGG 0.247675 2:+71425580 None:intergenic
ATATTCCTCTTCATTGAGTA+TGG 0.259965 2:+71427786 None:intergenic
GACTGTTGCATTTCAAATAT+TGG 0.260496 2:-71424959 MsaT010293.1:CDS
GGCAGTAGTGGATGTTTCTT+TGG 0.266653 2:-71427154 MsaT010293.1:CDS
TCCTTCCTTTGCTTGTCATC+AGG 0.267823 2:+71428933 None:intergenic
AATTCATCTTCACGGATTCT+TGG 0.284298 2:+71429258 None:intergenic
CACACGTGGACAACACTTTC+AGG 0.288181 2:-71426517 MsaT010293.1:CDS
TACAAGCAGCTTGTTAGTTC+TGG 0.292041 2:-71426873 MsaT010293.1:CDS
AAGGAACTGAGCAGAGAGTT+AGG 0.301450 2:-71428915 MsaT010293.1:CDS
ACAATGCCAGCTGGTCTTCC+TGG 0.308126 2:+71426445 None:intergenic
AGCCATTACAAGTGAAGTTT+TGG 0.309939 2:-71428887 MsaT010293.1:CDS
GTCTTTCACATTGTCGATCT+AGG 0.317831 2:+71426817 None:intergenic
GAACTCGGCATTGGTGGTTT+CGG 0.320296 2:-71427988 MsaT010293.1:CDS
TTGTACTGTTGAGAGGATTA+AGG 0.330586 2:-71424872 MsaT010293.1:CDS
TACATATCTCCTCCCATTCC+TGG 0.333263 2:+71427958 None:intergenic
AATCAAGAAGCTGCTAATTC+AGG 0.336353 2:-71429201 MsaT010293.1:CDS
AGGATTAAGGCTTCTTTGTC+TGG 0.337763 2:-71424859 MsaT010293.1:CDS
GCAACCAGAAGGCCTTCTTC+TGG 0.341248 2:+71427101 None:intergenic
GGAGGAGGTGTTGGTGAAAC+TGG 0.354741 2:-71425003 MsaT010293.1:CDS
TGTCCAGCTTCCATTAGATT+TGG 0.355568 2:+71429345 None:intergenic
TTTGGTGTGGTTGTCCCATC+TGG 0.359343 2:+71425027 None:intergenic
GTAATATCAACATGTTGCTT+TGG 0.360562 2:+71425181 None:intergenic
TTCTCTAAACCTCATGTTAT+CGG 0.361729 2:+71428686 None:intergenic
CCTTCCTTTGCTTGTCATCA+GGG 0.367415 2:+71428934 None:intergenic
ATTGCTAATGGAAGAGATAC+TGG 0.369741 2:-71425564 MsaT010293.1:CDS
GGAAGAAGTGCTACATAATC+TGG 0.371226 2:+71425069 None:intergenic
GTGCTATCAACTGGGGTGGC+AGG 0.373210 2:-71427433 MsaT010293.1:CDS
TTCGACAGTGCTACAAAATC+TGG 0.373686 2:-71429234 MsaT010293.1:CDS
TATGTAGCACTTCTTCCATC+TGG 0.375152 2:-71425063 MsaT010293.1:CDS
GGCAAAACTTTCTCTTGGTT+CGG 0.376070 2:-71424920 MsaT010293.1:CDS
CACTGGCTTTCCTACGTACT+TGG 0.377299 2:+71428059 None:intergenic
GTTAGTTCTGGACATGCATT+TGG 0.380690 2:-71426861 MsaT010293.1:CDS
CTGGATCTGGCAGAAGGACT+AGG 0.382500 2:+71427120 None:intergenic
GAATGTATACCTCTTCTCTA+AGG 0.384454 2:+71428568 None:intergenic
ACACTGGAAGTGCTATCAAC+TGG 0.386707 2:-71427442 MsaT010293.1:CDS
CTGGGTCATCTACACTCTTA+CGG 0.388294 2:+71426464 None:intergenic
GCAATGAATGTGGTTCTAAA+TGG 0.388547 2:-71425099 MsaT010293.1:CDS
GAAATGGCACATATTGCTAA+TGG 0.396088 2:-71425576 MsaT010293.1:CDS
ACATACTCTTTCTCCCATCT+TGG 0.396801 2:+71426599 None:intergenic
ACTCATTGGAATGGCACAAA+TGG 0.397042 2:-71427845 MsaT010293.1:CDS
GCAGTTGGTCCACCACAATT+AGG 0.400312 2:+71428642 None:intergenic
ATAACCTTTGAGTAGCCATT+TGG 0.403234 2:+71427063 None:intergenic
ACTGCTAGCTCAATTATCAT+TGG 0.404803 2:+71427883 None:intergenic
ATTTCAAATATTGGTTGATT+CGG 0.406456 2:-71424950 MsaT010293.1:CDS
AAATTTCCCCTAGGTTATAT+GGG 0.407762 2:-71426937 MsaT010293.1:intron
CTTTGGTTGCCTACACTTGA+AGG 0.408059 2:-71427814 MsaT010293.1:CDS
CTTTGCTTGTCATCAGGGTG+TGG 0.418881 2:+71428939 None:intergenic
TGGGTCATCTACACTCTTAC+GGG 0.423400 2:+71426465 None:intergenic
CAGCTTCCATTAGATTTGGC+TGG 0.429412 2:+71429349 None:intergenic
TCACCAACACCTCCTCCATT+TGG 0.438216 2:+71425009 None:intergenic
AATGTTCCAGCCAAATCTAA+TGG 0.438842 2:-71429355 MsaT010293.1:CDS
CCAACGGCAAAACTTTCTCT+TGG 0.441531 2:-71424925 MsaT010293.1:CDS
GCACAGGAGCTGATGATGTA+AGG 0.441905 2:-71426495 MsaT010293.1:CDS
AGCAACATGCTGATGGAACT+TGG 0.445791 2:-71427176 MsaT010293.1:CDS
CCTCTAGAACTCGGCATTGG+TGG 0.447065 2:-71427994 MsaT010293.1:CDS
TTCTCTTGGTTCGGTTGCTA+CGG 0.447827 2:-71424911 MsaT010293.1:CDS
AGAAGGCCTTCTTCTGGATC+TGG 0.453379 2:+71427107 None:intergenic
AACCAAAACTTCACTTGTAA+TGG 0.453525 2:+71428885 None:intergenic
ACAGCATCAGATCCAAGAAA+TGG 0.457226 2:-71429130 MsaT010293.1:CDS
TGAGCTAGCAGTTGCTGCTA+TGG 0.464050 2:-71427872 MsaT010293.1:CDS
TTCTTGGACAACTCCACCAT+TGG 0.466612 2:+71425596 None:intergenic
GATGGGACAACCACACCAAA+TGG 0.467628 2:-71425024 MsaT010293.1:CDS
CTCTCTCTAGTTGGCACAAG+TGG 0.472336 2:+71427219 None:intergenic
TGGTGGAGTTGTCCAAGAAA+TGG 0.474459 2:-71425592 MsaT010293.1:CDS
GGAGGAGATATGTATGGAGC+TGG 0.474886 2:-71427949 MsaT010293.1:CDS
TCAATGAAGAGGAATATGTT+AGG 0.476700 2:-71427780 MsaT010293.1:CDS
GGGCGAGGAGGAACCGATGA+AGG 0.476736 2:+71428015 None:intergenic
ACAAAATAACTCTCTCTAGT+TGG 0.477153 2:+71427210 None:intergenic
TTCGGAGGACAACCAGGAAT+GGG 0.477190 2:-71427970 MsaT010293.1:CDS
TCTTTCACATTGTCGATCTA+GGG 0.482839 2:+71426818 None:intergenic
TGTCGAATAATTCATCTTCA+CGG 0.483352 2:+71429250 None:intergenic
TCCTACGTACTTGGCAGCAA+TGG 0.502677 2:+71428068 None:intergenic
GAGAGTTGTACGGATTCAAC+AGG 0.503171 2:-71425156 MsaT010293.1:CDS
CGCCCTCTAGAACTCGGCAT+TGG 0.503902 2:-71427997 MsaT010293.1:CDS
CACTGGAAGTGCTATCAACT+GGG 0.506183 2:-71427441 MsaT010293.1:CDS
ATTGAATGCATGTTACTAGC+TGG 0.506437 2:+71429975 None:intergenic
AGATCCAGAAGAAGGCCTTC+TGG 0.506911 2:-71427105 MsaT010293.1:CDS
GGCGAGGAGGAACCGATGAA+GGG 0.507726 2:+71428016 None:intergenic
TTCATCTCTCTCTCTATCCC+AGG 0.507918 2:-71429910 MsaT010293.1:intron
CAATGCCAGCTGGTCTTCCT+GGG 0.508862 2:+71426446 None:intergenic
CTCATTGGAATGGCACAAAT+GGG 0.511283 2:-71427844 MsaT010293.1:CDS
CAGAGAAAACTGTGGACCAT+TGG 0.512102 2:+71427485 None:intergenic
TTAATTCAAGAAATGCCAAA+TGG 0.513645 2:-71427078 MsaT010293.1:CDS
TTTCGGAGGACAACCAGGAA+TGG 0.514423 2:-71427971 MsaT010293.1:CDS
TGCTATCAACTGGGGTGGCA+GGG 0.518257 2:-71427432 MsaT010293.1:CDS
AGGAACTGAGCAGAGAGTTA+GGG 0.519123 2:-71428914 MsaT010293.1:CDS
GCTGCTATGGAGGAACTCAT+TGG 0.520147 2:-71427859 MsaT010293.1:CDS
TTGTGGTTTATAAGAACCAA+TGG 0.522609 2:-71427501 MsaT010293.1:intron
TCCACTACTCTCCCCTTCAT+CGG 0.525289 2:-71428028 MsaT010293.1:CDS
TTCATTGCAACTATGTCTAC+AGG 0.525326 2:+71425114 None:intergenic
GGAATTGGTCCAAAACCTGC+TGG 0.527058 2:-71427745 MsaT010293.1:CDS
CAGCCAAATCTAATGGAAGC+TGG 0.529949 2:-71429348 MsaT010293.1:CDS
TGTGGGAATGTTTGTTGCCA+TGG 0.531917 2:+71426785 None:intergenic
GTATACCTCTTCTCTAAGGC+GGG 0.534397 2:+71428572 None:intergenic
GAACAAACGTACTCAAATGA+AGG 0.534903 2:-71428859 MsaT010293.1:intron
GGTGAAACTGGACACGGTGG+TGG 0.535930 2:-71424991 MsaT010293.1:CDS
GATATTACAAGAGAGTTGTA+CGG 0.536286 2:-71425166 MsaT010293.1:CDS
GCTTCACACTTAAAACCAGC+AGG 0.537419 2:+71427730 None:intergenic
CACAAATGGGTGATCCACTT+TGG 0.540052 2:-71427831 MsaT010293.1:CDS
GCGAGGAGGAACCGATGAAG+GGG 0.541185 2:+71428017 None:intergenic
GACATTTCTCCGATGGCAGT+TGG 0.544696 2:+71428627 None:intergenic
AGTAACATGCATTCAATGAT+CGG 0.546113 2:-71429969 MsaT010293.1:CDS
AAATTCCCGCCTTAGAGAAG+AGG 0.547209 2:-71428577 MsaT010293.1:intron
CTAGTTGGCACAAGTGGTGA+AGG 0.548358 2:+71427225 None:intergenic
GCTTCTTGTCCTAATTGTGG+TGG 0.548941 2:-71428651 MsaT010293.1:CDS
CTCGGCATTGGTGGTTTCGG+AGG 0.549851 2:-71427985 MsaT010293.1:CDS
GGAATGGGAGGAGATATGTA+TGG 0.550014 2:-71427955 MsaT010293.1:CDS
TTATTGCTTGTACTGTTGAG+AGG 0.553910 2:-71424879 MsaT010293.1:CDS
CCACCAATGCCGAGTTCTAG+AGG 0.555033 2:+71427994 None:intergenic
ATTGGTTGATTCGGTTCCAA+CGG 0.555616 2:-71424941 MsaT010293.1:CDS
ATCAAACGACATTTCTCCGA+TGG 0.559536 2:+71428620 None:intergenic
CTTCTGCCAGATCCAGAAGA+AGG 0.560942 2:-71427113 MsaT010293.1:CDS
ACACCAAATGGAGGAGGTGT+TGG 0.560944 2:-71425012 MsaT010293.1:CDS
CTTCTTCTGGATCTGGCAGA+AGG 0.561436 2:+71427114 None:intergenic
AACACCTCCTCCATTTGGTG+TGG 0.563793 2:+71425014 None:intergenic
GGATTTGCTATACTTCCAGA+TGG 0.564453 2:-71425042 MsaT010293.1:CDS
TATTGTAAGCAACATGCTGA+TGG 0.566221 2:-71427183 MsaT010293.1:CDS
ATGTGCATAAGCTTCTGTGC+TGG 0.567127 2:-71426559 MsaT010293.1:CDS
ATGGGTTGAGCATGTTGAAG+TGG 0.567383 2:-71426919 MsaT010293.1:CDS
TAGTGATAACAAACCAAGAT+GGG 0.567631 2:-71426612 MsaT010293.1:CDS
TGGATCTGGCAGAAGGACTA+GGG 0.567829 2:+71427121 None:intergenic
TCTTGAATTAAGCAACCAGA+AGG 0.568304 2:+71427090 None:intergenic
ATGCCGAGTTCTAGAGGGCG+AGG 0.568671 2:+71428000 None:intergenic
ATGCATTTGGTGCAAAACGT+TGG 0.570053 2:-71426848 MsaT010293.1:CDS
TGTGGTGGACCAACTGCCAT+CGG 0.570061 2:-71428636 MsaT010293.1:CDS
GGTGGTTTCGGAGGACAACC+AGG 0.570493 2:-71427976 MsaT010293.1:CDS
GATGACCCAGGAAGACCAGC+TGG 0.571107 2:-71426451 MsaT010293.1:CDS
TTAGTGATAACAAACCAAGA+TGG 0.574822 2:-71426613 MsaT010293.1:intron
ATTGGTTTATCAGCTTCAGT+AGG 0.576989 2:+71427901 None:intergenic
TAACCTTGTTGAGATTCTCA+TGG 0.576995 2:-71427671 MsaT010293.1:CDS
CACAGGAGCTGATGATGTAA+GGG 0.577558 2:-71426494 MsaT010293.1:CDS
GCACTTAGAACAATGCCAGC+TGG 0.578109 2:+71426436 None:intergenic
ACCGATGAAGGGGAGAGTAG+TGG 0.579805 2:+71428027 None:intergenic
GATCTTCTTAGATCAGTAAG+TGG 0.581313 2:-71427925 MsaT010293.1:CDS
CCTCCTCGCCCTCTAGAACT+CGG 0.581323 2:-71428003 MsaT010293.1:CDS
AACATTCCCACAGTCGATGT+TGG 0.581462 2:-71426774 MsaT010293.1:CDS
TGTCCACGTGTGTGCTGTTG+AGG 0.582831 2:+71426528 None:intergenic
TGAATGTGGTTCTAAATGGA+GGG 0.584402 2:-71425095 MsaT010293.1:CDS
TGTTGAGATTCTCATGGATG+TGG 0.587472 2:-71427665 MsaT010293.1:intron
TGGGATATCCTTTCCAATGG+TGG 0.588001 2:-71425609 MsaT010293.1:CDS
CGTTTGATGAACATCACTTG+AGG 0.589246 2:-71428605 MsaT010293.1:CDS
AATGCTTCTTGTCCTAATTG+TGG 0.590261 2:-71428654 MsaT010293.1:CDS
TGATTGTGAAATATGCAAGC+TGG 0.590342 2:-71430008 None:intergenic
GGATCTGATGCTGTGTATGA+CGG 0.590628 2:+71429139 None:intergenic
CCCTGATGACAAGCAAAGGA+AGG 0.591678 2:-71428934 MsaT010293.1:CDS
AGTAGTGGATAGCTCACCAC+TGG 0.592239 2:+71428042 None:intergenic
GCTAGCAGTTGCTGCTATGG+AGG 0.593019 2:-71427869 MsaT010293.1:CDS
TGGACAACACTTTCAGGCAC+AGG 0.595843 2:-71426511 MsaT010293.1:CDS
TGTATACCTCTTCTCTAAGG+CGG 0.598180 2:+71428571 None:intergenic
GCAACATGCTGATGGAACTT+GGG 0.599510 2:-71427175 MsaT010293.1:CDS
ATGGAACCTCCTTCAAGTGT+AGG 0.599518 2:+71427805 None:intergenic
GAATGTGGTTCTAAATGGAG+GGG 0.601539 2:-71425094 MsaT010293.1:CDS
GGACAACTCCACCATTGGAA+AGG 0.603145 2:+71425601 None:intergenic
AATGCCAAATGGCTACTCAA+AGG 0.605118 2:-71427067 MsaT010293.1:intron
TGTCTCAAGAGCAATGACAC+TGG 0.606529 2:-71427458 MsaT010293.1:CDS
ATTCCCACAGTCGATGTTGG+CGG 0.607641 2:-71426771 MsaT010293.1:intron
GGTACCGCCAACATCGACTG+TGG 0.611425 2:+71426767 None:intergenic
CACCAATGCCGAGTTCTAGA+GGG 0.615823 2:+71427995 None:intergenic
AACTTCGTGCCGATAACATG+AGG 0.617798 2:-71428695 MsaT010293.1:CDS
CACACCCTGATGACAAGCAA+AGG 0.619885 2:-71428938 MsaT010293.1:CDS
CAGTGGGATATCCTTTCCAA+TGG 0.619986 2:-71425612 MsaT010293.1:CDS
GGAAGTATAGCAAATCCAGA+TGG 0.620130 2:+71425048 None:intergenic
AGGTTCCATACTCAATGAAG+AGG 0.621981 2:-71427791 MsaT010293.1:CDS
TATGGAGGAACTCATTGGAA+TGG 0.622262 2:-71427854 MsaT010293.1:CDS
TGATGGAACTTGGGCAGTAG+TGG 0.623796 2:-71427166 MsaT010293.1:CDS
GTTTGCAGACTCAACAGGAG+AGG 0.625915 2:-71428752 MsaT010293.1:intron
GATTTGCTATACTTCCAGAT+GGG 0.631821 2:-71425041 MsaT010293.1:CDS
TAACGTTTCTTCTTAGCACG+AGG 0.632532 2:+71429165 None:intergenic
GGAAGTGCTATCAACTGGGG+TGG 0.632566 2:-71427437 MsaT010293.1:CDS
GGAGGACAACCAGGAATGGG+AGG 0.639321 2:-71427967 MsaT010293.1:CDS
TCAAGTGTAGGCAACCAAAG+TGG 0.640121 2:+71427817 None:intergenic
CTACAATGGTGCATTACAAG+TGG 0.640429 2:-71427407 MsaT010293.1:intron
GTACCGCCAACATCGACTGT+GGG 0.642719 2:+71426768 None:intergenic
ACAACCACACCAAATGGAGG+AGG 0.644018 2:-71425018 MsaT010293.1:CDS
GTGCCTCAACAGCACACACG+TGG 0.644853 2:-71426531 MsaT010293.1:CDS
ATGAATGTGGTTCTAAATGG+AGG 0.647763 2:-71425096 MsaT010293.1:CDS
GGGGTGGCAGGGAACTACAA+TGG 0.649866 2:-71427421 MsaT010293.1:CDS
TGGTTGCCTACACTTGAAGG+AGG 0.652894 2:-71427811 MsaT010293.1:CDS
GGAAGAAACAACAATAACGT+TGG 0.654744 2:-71429948 MsaT010293.1:CDS
AGATGAAAACTCAAGAAGTG+AGG 0.655242 2:-71426359 MsaT010293.1:intron
CATGTTGAAGTGGATGATAG+AGG 0.665308 2:-71426909 MsaT010293.1:CDS
GGGACAACCACACCAAATGG+AGG 0.673800 2:-71425021 MsaT010293.1:CDS
CATCCATGAGAATCTCAACA+AGG 0.674971 2:+71427668 None:intergenic
CGTAAGAGTGTAGATGACCC+AGG 0.679064 2:-71426463 MsaT010293.1:CDS
GCCATTGCTGCCAAGTACGT+AGG 0.683580 2:-71428069 MsaT010293.1:CDS
CCGAGTTCTAGAGGGCGAGG+AGG 0.684847 2:+71428003 None:intergenic
TGAAAGACTTGCTAGTGCCA+TGG 0.696212 2:-71426802 MsaT010293.1:CDS
CAAGTACGTAGGAAAGCCAG+TGG 0.711066 2:-71428058 MsaT010293.1:CDS
AGACATAGTTGCAATGAATG+TGG 0.720864 2:-71425109 MsaT010293.1:CDS
GACAATGCCAGAGAAAACTG+TGG 0.735059 2:+71427477 None:intergenic
ACTGGAAGTGCTATCAACTG+GGG 0.765115 2:-71427440 MsaT010293.1:CDS
GTTGGTGAAACTGGACACGG+TGG 0.765833 2:-71424994 MsaT010293.1:CDS
GGTGTTGGTGAAACTGGACA+CGG 0.766330 2:-71424997 MsaT010293.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 71424846 71430018 71424846 ID=MsaG010293
Chr2 mRNA 71424846 71430018 71424846 ID=MsaT010293.1;Parent=MsaG010293
Chr2 exon 71424846 71425217 71424846 ID=MsaT010293.1.exon11;Parent=MsaT010293.1
Chr2 CDS 71424846 71425217 71424846 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71425536 71425631 71425536 ID=MsaT010293.1.exon10;Parent=MsaT010293.1
Chr2 CDS 71425536 71425631 71425536 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71426360 71426631 71426360 ID=MsaT010293.1.exon9;Parent=MsaT010293.1
Chr2 CDS 71426360 71426631 71426360 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71426772 71426946 71426772 ID=MsaT010293.1.exon8;Parent=MsaT010293.1
Chr2 CDS 71426772 71426946 71426772 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71427068 71427268 71427068 ID=MsaT010293.1.exon7;Parent=MsaT010293.1
Chr2 CDS 71427068 71427268 71427068 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71427408 71427509 71427408 ID=MsaT010293.1.exon6;Parent=MsaT010293.1
Chr2 CDS 71427408 71427509 71427408 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71427666 71428106 71427666 ID=MsaT010293.1.exon5;Parent=MsaT010293.1
Chr2 CDS 71427666 71428106 71427666 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71428578 71428766 71428578 ID=MsaT010293.1.exon4;Parent=MsaT010293.1
Chr2 CDS 71428578 71428766 71428578 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71428860 71428977 71428860 ID=MsaT010293.1.exon3;Parent=MsaT010293.1
Chr2 CDS 71428860 71428977 71428860 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71429126 71429379 71429126 ID=MsaT010293.1.exon2;Parent=MsaT010293.1
Chr2 CDS 71429126 71429379 71429126 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Chr2 exon 71429911 71430018 71429911 ID=MsaT010293.1.exon1;Parent=MsaT010293.1
Chr2 CDS 71429911 71430018 71429911 ID=cds.MsaT010293.1;Parent=MsaT010293.1
Gene Sequence

>MsaT010293.1

ATGCAAGCTGGTTTAATGATTCCAGCTAGTAACATGCATTCAATGATCGGAAGAAACAACAATAACGTTGGTGTCTTTGGTTTATCTTCATCTCTCTCTCTATCCCAGAAAATGTTCCAGCCAAATCTAATGGAAGCTGGACAACATCATCAACTTCTGCCATTTGACATGACACAAAACAACAACAACACTTCAGAAAGTGATGTTCCAAGAATCCGTGAAGATGAATTATTCGACAGTGCTACAAAATCTGGTAACAGTGAAAATCAAGAAGCTGCTAATTCAGGTGAAGATCAAGAGCCTCGTGCTAAGAAGAAACGTTATCACCGTCATACACAGCATCAGATCCAAGAAATGGAAGCGTTTTTTAAGGAATGTCCACACCCTGATGACAAGCAAAGGAAGGAACTGAGCAGAGAGTTAGGGTTAGAGCCATTACAAGTGAAGTTTTGGTTTCAGAACAAACGTACTCAAATGAAGACTCAACAGGAGAGGCACGAGAATACTTCACTTAGAACTGAGAATGAGAAACTTCGTGCCGATAACATGAGGTTTAGAGAAGCTCTAAGTAATGCTTCTTGTCCTAATTGTGGTGGACCAACTGCCATCGGAGAAATGTCGTTTGATGAACATCACTTGAGGCTTGAAAATTCCCGCCTTAGAGAAGAGATTGATAGGATATCTGCCATTGCTGCCAAGTACGTAGGAAAGCCAGTGGTGAGCTATCCACTACTCTCCCCTTCATCGGTTCCTCCTCGCCCTCTAGAACTCGGCATTGGTGGTTTCGGAGGACAACCAGGAATGGGAGGAGATATGTATGGAGCTGGAGATCTTCTTAGATCAGTAAGTGGACCTACTGAAGCTGATAAACCAATGATAATTGAGCTAGCAGTTGCTGCTATGGAGGAACTCATTGGAATGGCACAAATGGGTGATCCACTTTGGTTGCCTACACTTGAAGGAGGTTCCATACTCAATGAAGAGGAATATGTTAGGTCTTTTCCTAGAGGAATTGGTCCAAAACCTGCTGGTTTTAAGTGTGAAGCTTCAAGAGAATCTTCTGTTGTCATCATGAATCATGTTAACCTTGTTGAGATTCTCATGGATGTGAACCAATGGTCCACAGTTTTCTCTGGCATTGTCTCAAGAGCAATGACACTGGAAGTGCTATCAACTGGGGTGGCAGGGAACTACAATGGTGCATTACAAGTGATGACAGCAGAACTGCAAGTACCTTCACCACTTGTGCCAACTAGAGAGAGTTATTTTGTGAGGTATTGTAAGCAACATGCTGATGGAACTTGGGCAGTAGTGGATGTTTCTTTGGACAATTTACGCCCTAGTCCTTCTGCCAGATCCAGAAGAAGGCCTTCTGGTTGCTTAATTCAAGAAATGCCAAATGGCTACTCAAAGGTTATATGGGTTGAGCATGTTGAAGTGGATGATAGAGGTGTTCATAATCTATACAAGCAGCTTGTTAGTTCTGGACATGCATTTGGTGCAAAACGTTGGATTGCAACCCTAGATCGACAATGTGAAAGACTTGCTAGTGCCATGGCAACAAACATTCCCACAGTCGATGTTGGCGTGATAACAAACCAAGATGGGAGAAAGAGTATGTTGAAACTAGCTGAAAGAATGTGCATAAGCTTCTGTGCTGGAGTGAGTGCCTCAACAGCACACACGTGGACAACACTTTCAGGCACAGGAGCTGATGATGTAAGGGTCATGACCCGTAAGAGTGTAGATGACCCAGGAAGACCAGCTGGCATTGTTCTAAGTGCTGCAACTTCTTTTTGGCTTCCAGTGCCACCAAAAAGAGTTTTTGAATTTCTTAGAGATGAAAACTCAAGAAGTGAGTGGGATATCCTTTCCAATGGTGGAGTTGTCCAAGAAATGGCACATATTGCTAATGGAAGAGATACTGGAAATTGTGTTTCTCTACTTCGAGTGAATAGTGCAAATTCAAGCCAAAGCAACATGTTGATATTACAAGAGAGTTGTACGGATTCAACAGGATCATTTGTGATATATGCACCTGTAGACATAGTTGCAATGAATGTGGTTCTAAATGGAGGGGATCCAGATTATGTAGCACTTCTTCCATCTGGATTTGCTATACTTCCAGATGGGACAACCACACCAAATGGAGGAGGTGTTGGTGAAACTGGACACGGTGGTGGATCTCTTTTGACTGTTGCATTTCAAATATTGGTTGATTCGGTTCCAACGGCAAAACTTTCTCTTGGTTCGGTTGCTACGGTTAATAATCTTATTGCTTGTACTGTTGAGAGGATTAAGGCTTCTTTGTCTGGTGAAGTTGCTTGA
Protein sequence

>MsaT010293.1

MQAGLMIPASNMHSMIGRNNNNVGVFGLSSSLSLSQKMFQPNLMEAGQHHQLLPFDMTQNNNNTSESDVPRIREDELFDSATKSGNSENQEAANSGEDQEPRAKKKRYHRHTQHQIQEMEAFFKECPHPDDKQRKELSRELGLEPLQVKFWFQNKRTQMKTQQERHENTSLRTENEKLRADNMRFREALSNASCPNCGGPTAIGEMSFDEHHLRLENSRLREEIDRISAIAAKYVGKPVVSYPLLSPSSVPPRPLELGIGGFGGQPGMGGDMYGAGDLLRSVSGPTEADKPMIIELAVAAMEELIGMAQMGDPLWLPTLEGGSILNEEEYVRSFPRGIGPKPAGFKCEASRESSVVIMNHVNLVEILMDVNQWSTVFSGIVSRAMTLEVLSTGVAGNYNGALQVMTAELQVPSPLVPTRESYFVRYCKQHADGTWAVVDVSLDNLRPSPSARSRRRPSGCLIQEMPNGYSKVIWVEHVEVDDRGVHNLYKQLVSSGHAFGAKRWIATLDRQCERLASAMATNIPTVDVGVITNQDGRKSMLKLAERMCISFCAGVSASTAHTWTTLSGTGADDVRVMTRKSVDDPGRPAGIVLSAATSFWLPVPPKRVFEFLRDENSRSEWDILSNGGVVQEMAHIANGRDTGNCVSLLRVNSANSSQSNMLILQESCTDSTGSFVIYAPVDIVAMNVVLNGGDPDYVALLPSGFAILPDGTTTPNGGGVGETGHGGGSLLTVAFQILVDSVPTAKLSLGSVATVNNLIACTVERIKASLSGEVA*