AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010314


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010314 MtrunA17_Chr2g0323031 90.769 260 4 2 1 240 1 260 8.73e-171 471
MsaG010314 MtrunA17_Chr4g0042041 54.812 239 88 7 12 240 9 237 2.59e-77 233
MsaG010314 MtrunA17_Chr2g0319301 53.750 240 82 8 12 240 9 230 3.95e-74 224
MsaG010314 MtrunA17_Chr1g0167821 48.824 170 85 2 73 240 77 246 1.47e-44 149
MsaG010314 MtrunA17_Chr1g0161461 43.478 92 51 1 90 181 82 172 8.33e-18 81.3
MsaG010314 MtrunA17_Chr4g0038511 43.299 97 54 1 88 184 9 104 8.52e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010314 AT5G54680.1 52.315 216 86 7 36 240 25 234 2.61e-65 202
MsaG010314 AT1G51070.1 54.404 193 80 5 56 240 34 226 8.40e-64 198
MsaG010314 AT1G51070.2 54.124 194 81 5 55 240 99 292 1.06e-62 198
MsaG010314 AT4G14410.2 50.888 169 71 5 78 240 115 277 7.67e-43 146
MsaG010314 AT4G14410.3 50.888 169 71 5 78 240 115 277 7.67e-43 146
MsaG010314 AT4G14410.1 50.888 169 71 5 78 240 121 283 9.22e-43 146
MsaG010314 AT3G23210.2 44.495 218 104 6 31 240 83 291 2.68e-40 140
MsaG010314 AT3G23210.1 44.495 218 104 6 31 240 112 320 4.08e-40 140
MsaG010314 AT3G19860.2 45.745 94 50 1 88 181 61 153 6.23e-17 79.0
MsaG010314 AT3G19860.1 44.898 98 53 1 84 181 4 100 7.43e-17 78.2
MsaG010314 AT3G19860.3 44.898 98 53 1 84 181 35 131 9.67e-17 78.2
MsaG010314 AT4G36060.2 43.750 96 53 1 86 181 27 121 2.20e-16 76.6
MsaG010314 AT4G36060.4 43.750 96 53 1 86 181 27 121 2.25e-16 76.6
MsaG010314 AT4G36060.3 43.750 96 53 1 86 181 45 139 4.56e-16 75.9
MsaG010314 AT4G36060.1 43.750 96 53 1 86 181 45 139 4.93e-16 75.9

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATGATTTAAAAGGTTTA+TGG 0.124834 2:-71666225 MsaT010314.1:intron
TCTCAGCTTCATTTCTTAAT+TGG 0.176160 2:+71666120 None:intergenic
TAAGCTAACCTTGTCTGTTT+TGG 0.191189 2:+71666169 None:intergenic
TATTTCTGCTGCTGATTTCA+TGG 0.212247 2:-71667471 MsaT010314.1:CDS
TCCATTTGGCTCTATGATTA+TGG 0.214517 2:-71667509 MsaT010314.1:CDS
CCACTGGTGGTTCCTCCATT+TGG 0.268940 2:-71667523 MsaT010314.1:CDS
CTCAGCTTCATTTCTTAATT+GGG 0.281253 2:+71666121 None:intergenic
TTACAACAATATAATGGATA+TGG 0.295671 2:-71667549 None:intergenic
ATCACGAAGCTCTTTCTTCT+CGG 0.320152 2:+71665387 None:intergenic
CACTTTCTCACGACCTGCCT+TGG 0.343619 2:+71666339 None:intergenic
TATTTCAAAGTCACCTCATA+TGG 0.359098 2:+71667366 None:intergenic
ATGCCTTTCATTGGTTATCC+TGG 0.370469 2:-71665241 MsaT010314.1:CDS
TGCTAACCACTTTAAGCAAC+TGG 0.372348 2:+71665151 None:intergenic
CTGCTGCTCCTGCTTTCACC+TGG 0.384655 2:-71667436 MsaT010314.1:CDS
TTATATTAGCAGCATGGAAA+TGG 0.384979 2:-71666759 MsaT010314.1:intron
CACAAGCTGATGCCTTTCAT+TGG 0.397178 2:-71665250 MsaT010314.1:CDS
AACTGCCACATTGAGATTCC+AGG 0.422338 2:+71665223 None:intergenic
AAGATATATTACCGCTTTGA+AGG 0.433191 2:+71666701 None:intergenic
CTCACTGGATTCAACAGTAC+TGG 0.435458 2:-71666732 MsaT010314.1:CDS
TCCTTTAGCAGCCATGGCAT+TGG 0.447269 2:+71665285 None:intergenic
ACCCTCCTTCCTCCCATATG+AGG 0.459345 2:-71667379 MsaT010314.1:intron
GAAGCTAGACAAAGAAAAGT+TGG 0.461043 2:-71665350 MsaT010314.1:CDS
AAGCAACTGGAGGTCGAAGC+AGG 0.492222 2:+71665164 None:intergenic
TCCAATGCCATGGCTGCTAA+AGG 0.493863 2:-71665286 MsaT010314.1:CDS
TGAGGCATCCAGGTGAAAGC+AGG 0.497970 2:+71667428 None:intergenic
GAAGGGATAAATTGAATGAC+AGG 0.502758 2:-71666315 MsaT010314.1:intron
AGTTTGTCCTTTAGCAGCCA+TGG 0.506775 2:+71665279 None:intergenic
AGACTGAGGCTGAGGCATCC+AGG 0.526908 2:+71667418 None:intergenic
CATTGGAGAGGAAGTTAGAC+TGG 0.527913 2:+71665302 None:intergenic
GCCATAATCATAGAGCCAAA+TGG 0.530360 2:+71667508 None:intergenic
CATGGAAATGGAATACTCAC+TGG 0.536132 2:-71666747 MsaT010314.1:CDS
GATTCAACAGTACTGGAAAA+CGG 0.545991 2:-71666725 MsaT010314.1:CDS
AGATATATTACCGCTTTGAA+GGG 0.546831 2:+71666702 None:intergenic
ATTTCAAAGTCACCTCATAT+GGG 0.549756 2:+71667367 None:intergenic
ACCTCATATGGGAGGAAGGA+GGG 0.560123 2:+71667378 None:intergenic
CGCTACAGTTGATACATCAC+AGG 0.567747 2:-71665191 MsaT010314.1:CDS
TGAGTTTGAGACTGAGGCTG+AGG 0.571581 2:+71667410 None:intergenic
TATGCATCTAGCTCCAAGGC+AGG 0.594326 2:-71666352 MsaT010314.1:CDS
CAATGAAAGGCATCAGCTTG+TGG 0.597079 2:+71665251 None:intergenic
AAGCTGAGAGGCTCAACGAA+AGG 0.598466 2:-71666106 MsaT010314.1:CDS
AGTCACCTCATATGGGAGGA+AGG 0.599006 2:+71667374 None:intergenic
GGTTAGCTTATTAAATGATG+CGG 0.600148 2:-71666156 MsaT010314.1:CDS
ATTCCAGGATAACCAATGAA+AGG 0.600579 2:+71665238 None:intergenic
ATTAAATGATGCGGTTCGAG+TGG 0.605944 2:-71666147 MsaT010314.1:CDS
CCAAATGGAGGAACCACCAG+TGG 0.607270 2:+71667523 None:intergenic
TAACTTCCTCTCCAATGCCA+TGG 0.610860 2:-71665296 MsaT010314.1:CDS
ATGGATATGGATTCCACTGG+TGG 0.610998 2:-71667536 MsaT010314.1:CDS
ATCATATGCATCTAGCTCCA+AGG 0.614949 2:-71666356 MsaT010314.1:CDS
TAGCAGCCATGGCATTGGAG+AGG 0.631338 2:+71665290 None:intergenic
TGTGATGTATCAACTGTAGC+GGG 0.634621 2:+71665193 None:intergenic
AGGTCGTGAGAAAGTGCGAA+GGG 0.647351 2:-71666332 MsaT010314.1:CDS
ATGATATGAGTTTGAGACTG+AGG 0.651321 2:+71667404 None:intergenic
CACCTCATATGGGAGGAAGG+AGG 0.652615 2:+71667377 None:intergenic
CAGGTCGTGAGAAAGTGCGA+AGG 0.654075 2:-71666333 MsaT010314.1:CDS
TCAAAGTCACCTCATATGGG+AGG 0.655796 2:+71667370 None:intergenic
ATAATGGATATGGATTCCAC+TGG 0.660012 2:-71667539 MsaT010314.1:CDS
TAACCACTTTAAGCAACTGG+AGG 0.675285 2:+71665154 None:intergenic
ATAATCATAGAGCCAAATGG+AGG 0.683773 2:+71667511 None:intergenic
TACGCTGCCCAAAACAGACA+AGG 0.685974 2:-71666177 MsaT010314.1:CDS
CTGTGATGTATCAACTGTAG+CGG 0.693038 2:+71665192 None:intergenic
TGGAAAACGGCCCTTCAAAG+CGG 0.713168 2:-71666712 MsaT010314.1:intron
AATTAAGAAATGAAGCTGAG+AGG 0.714845 2:-71666118 MsaT010314.1:CDS
GTTATCCTGGAATCTCAATG+TGG 0.748590 2:-71665228 MsaT010314.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 71665162 71667558 71665162 ID=MsaG010314
Chr2 mRNA 71665162 71667558 71665162 ID=MsaT010314.1;Parent=MsaG010314
Chr2 exon 71665162 71665410 71665162 ID=MsaT010314.1.exon5;Parent=MsaT010314.1
Chr2 CDS 71665162 71665410 71665162 ID=cds.MsaT010314.1;Parent=MsaT010314.1
Chr2 exon 71666070 71666232 71666070 ID=MsaT010314.1.exon4;Parent=MsaT010314.1
Chr2 CDS 71666070 71666232 71666070 ID=cds.MsaT010314.1;Parent=MsaT010314.1
Chr2 exon 71666316 71666390 71666316 ID=MsaT010314.1.exon3;Parent=MsaT010314.1
Chr2 CDS 71666316 71666390 71666316 ID=cds.MsaT010314.1;Parent=MsaT010314.1
Chr2 exon 71666713 71666769 71666713 ID=MsaT010314.1.exon2;Parent=MsaT010314.1
Chr2 CDS 71666713 71666769 71666713 ID=cds.MsaT010314.1;Parent=MsaT010314.1
Chr2 exon 71667380 71667558 71667380 ID=MsaT010314.1.exon1;Parent=MsaT010314.1
Chr2 CDS 71667380 71667558 71667380 ID=cds.MsaT010314.1;Parent=MsaT010314.1
Gene Sequence

>MsaT010314.1

ATGGATATGGATTCCACTGGTGGTTCCTCCATTTGGCTCTATGATTATGGCTATGATGATATATCTATTTCTGCTGCTGATTTCATGGCTTCTGACTCTTCTGCTGCTCCTGCTTTCACCTGGATGCCTCAGCCTCAGTCTCAAACTCATATCATCAACCCTCCTTCCTCCCATATGAGCATGGAAATGGAATACTCACTGGATTCAACAGTACTGGAAAACGGCCCTTCAAAGCGGTTAAGGACAGAATCATATGCATCTAGCTCCAAGGCAGGTCGTGAGAAAGTGCGAAGGGATAAATTGAATGACAGGTTTATGGAATTGAGTTCTGTCTTAGAGCCTGATACGCTGCCCAAAACAGACAAGGTTAGCTTATTAAATGATGCGGTTCGAGTGGTGACCCAATTAAGAAATGAAGCTGAGAGGCTCAACGAAAGGAATGATGAATTGCGCGAAAAAGTTAAAGAACTTAAGGCCGAGAAGAAAGAGCTTCGTGATGAAAAAAATAAGCTGAAGCTAGACAAAGAAAAGTTGGAACAGCAAGTCAAATTAGCAAGTGTCCAGTCTAACTTCCTCTCCAATGCCATGGCTGCTAAAGGACAAACTGCTAACCACAAGCTGATGCCTTTCATTGGTTATCCTGGAATCTCAATGTGGCAGTTTATGTCACCCGCTACAGTTGATACATCACAGGATCACCTGCTTCGACCTCCAGTTGCTTAA

Protein sequence

>MsaT010314.1

MDMDSTGGSSIWLYDYGYDDISISAADFMASDSSAAPAFTWMPQPQSQTHIINPPSSHMSMEMEYSLDSTVLENGPSKRLRTESYASSSKAGREKVRRDKLNDRFMELSSVLEPDTLPKTDKVSLLNDAVRVVTQLRNEAERLNERNDELREKVKELKAEKKELRDEKNKLKLDKEKLEQQVKLASVQSNFLSNAMAAKGQTANHKLMPFIGYPGISMWQFMSPATVDTSQDHLLRPPVA*