AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010455

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010455 MtrunA17_Chr2g0331371 81.592 201 29 1 1 201 1 193 1.53e-105 311
MsaG010455 MtrunA17_Chr6g0473421 100.000 131 0 0 1 131 1 131 2.86e-92 275
MsaG010455 MtrunA17_Chr8g0389811 53.788 132 61 0 34 165 63 194 3.73e-46 158
MsaG010455 MtrunA17_Chr1g0203051 47.134 157 83 0 36 192 60 216 7.69e-46 167
MsaG010455 MtrunA17_Chr1g0172931 61.538 104 40 0 34 137 55 158 5.70e-42 145
MsaG010455 MtrunA17_Chr1g0171741 36.905 168 103 2 20 185 96 262 1.96e-29 115
MsaG010455 MtrunA17_Chr2g0287651 32.143 140 95 0 46 185 66 205 1.58e-17 85.1
MsaG010455 MtrunA17_Chr2g0287651 28.780 205 138 3 188 392 549 745 2.43e-14 75.1
MsaG010455 MtrunA17_Chr2g0308841 36.264 91 58 0 178 268 134 224 4.35e-17 80.9
MsaG010455 MtrunA17_Chr2g0290391 36.220 127 78 2 67 191 63 188 1.17e-16 82.4
MsaG010455 MtrunA17_Chr1g0198761 34.058 138 81 3 67 195 59 195 1.31e-16 82.4
MsaG010455 MtrunA17_Chr2g0276841 33.058 121 76 3 61 177 76 195 1.01e-13 73.2
MsaG010455 MtrunA17_Chr8g0366311 47.170 53 28 0 34 86 58 110 4.02e-13 65.5
MsaG010455 MtrunA17_Chr5g0448761 40.945 127 67 4 51 173 58 180 1.55e-12 69.7
MsaG010455 MtrunA17_Chr5g0431001 31.081 148 87 5 66 199 72 218 1.88e-12 68.9
MsaG010455 MtrunA17_Chr6g0458391 38.298 94 54 3 67 159 237 327 2.32e-11 65.9
MsaG010455 MtrunA17_Chr3g0082121 30.000 120 81 2 57 175 45 162 6.06e-11 64.3
MsaG010455 MtrunA17_Chr2g0329361 40.000 95 53 3 67 159 73 165 7.02e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010455 AT5G28530.4 35.433 127 79 2 67 191 67 192 8.70e-19 89.4
MsaG010455 AT5G28530.5 35.433 127 79 2 67 191 67 192 9.55e-19 89.0
MsaG010455 AT5G28530.3 35.433 127 79 2 67 191 67 192 1.08e-18 89.0
MsaG010455 AT5G28530.2 35.433 127 79 2 67 191 67 192 1.08e-18 89.0
MsaG010455 AT5G28530.1 35.433 127 79 2 67 191 67 192 1.14e-18 89.0
MsaG010455 AT1G10240.2 32.308 130 83 3 66 191 59 187 4.30e-12 68.6
MsaG010455 AT1G10240.1 32.308 130 83 3 66 191 59 187 4.30e-12 68.6
MsaG010455 AT4G38180.1 40.000 130 67 6 47 171 67 190 1.66e-11 66.6

Find 66 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATAAGCCACTGCAATATT+TGG 0.202241 2:+73597980 None:intergenic
TTCTTACTGGCCATCGAGTA+TGG 0.284382 2:-73596490 MsaT010455.1:CDS
TTGCATCAGTTTATGGAGTT+TGG 0.292111 2:-73598118 MsaT010455.1:intron
AGTGATATGAAAGTTGGATT+TGG 0.317586 2:-73598085 MsaT010455.1:CDS
GTGAATGGAATGTTTCTTAC+TGG 0.321653 2:-73596503 MsaT010455.1:CDS
ATTGAAGGAATGGAAGATAT+AGG 0.332722 2:-73598055 MsaT010455.1:CDS
CAAGTCTGCGGAAGATTATC+TGG 0.344895 2:-73596223 MsaT010455.1:CDS
TGTACATATAGTTTCTGTTA+TGG 0.362635 2:-73596421 MsaT010455.1:CDS
GTAGTCAACGTTGGTATGTA+TGG 0.377945 2:-73597742 MsaT010455.1:CDS
ATCAATGTGCACACTACATT+TGG 0.382053 2:+73597769 None:intergenic
TTGGATTTCTTCCGGGTCAC+AGG 0.383991 2:-73597673 MsaT010455.1:CDS
TGTAGTGTGCACATTGATTC+TGG 0.404970 2:-73597764 MsaT010455.1:CDS
GGTTGAAATACCATCGTGTT+TGG 0.406628 2:-73596385 MsaT010455.1:CDS
AATTGGTATGCTCGAATGAA+TGG 0.408021 2:-73597950 MsaT010455.1:CDS
TTGAAGGAATGGAAGATATA+GGG 0.408538 2:-73598054 MsaT010455.1:CDS
TTCGGTTCTGTTTGCAACAT+TGG 0.417204 2:+73596128 None:intergenic
CGTGTTTGGTTCTTGATAGA+TGG 0.419798 2:-73596371 MsaT010455.1:CDS
CAGCTCTTGCCTCACGCTTA+CGG 0.421899 2:+73597813 None:intergenic
ATAGAAAGTGATATGAAAGT+TGG 0.423663 2:-73598091 MsaT010455.1:CDS
ACTCTGAAGATGATGAATAA+AGG 0.431741 2:-73595897 None:intergenic
TGGATCTTGAATAAATTGTT+CGG 0.449685 2:+73596110 None:intergenic
GGATTTGGATTTGCTATTGA+AGG 0.453071 2:-73598070 MsaT010455.1:CDS
AAGCTTTAAATCAAAGATCA+AGG 0.454195 2:-73596266 MsaT010455.1:CDS
CTGCCTTCGAATGGAATCAA+TGG 0.464430 2:-73596454 MsaT010455.1:CDS
ACTAAGGGGCGTGGTGGTGC+AGG 0.465724 2:-73596075 MsaT010455.1:CDS
ATCAAGGGATGTGAATGGTT+TGG 0.468442 2:-73596250 MsaT010455.1:CDS
CATTGATTCCATTCGAAGGC+AGG 0.472988 2:+73596455 None:intergenic
TGTGGTAAATGTGGAATTGT+TGG 0.474301 2:-73596015 MsaT010455.1:CDS
TGGATTTCTTCCGGGTCACA+GGG 0.475656 2:-73597672 MsaT010455.1:CDS
GTCATGTATCTGATGAAGAT+TGG 0.475848 2:-73595921 MsaT010455.1:CDS
AGTATCACAGTGCATTGGTT+GGG 0.490302 2:-73596313 MsaT010455.1:CDS
CTGCTCGTAGAAGCAAAGTA+AGG 0.491172 2:-73597922 MsaT010455.1:CDS
ATTGTAGACAAGGAAGTGAA+TGG 0.502388 2:-73596518 MsaT010455.1:CDS
TGGATTTGCTATTGAAGGAA+TGG 0.513659 2:-73598065 MsaT010455.1:CDS
CACATTTACCACAATGTCCA+CGG 0.523480 2:+73596025 None:intergenic
TGCCTTCGAATGGAATCAAT+GGG 0.524924 2:-73596453 MsaT010455.1:CDS
AGCTTCTGCTGTCACAGTGA+AGG 0.542920 2:-73596586 MsaT010455.1:CDS
CAAATGTTCCTGCCTTCGAA+TGG 0.550428 2:-73596463 MsaT010455.1:CDS
GATTTCTTCCGGGTCACAGG+GGG 0.562392 2:-73597670 MsaT010455.1:CDS
TAGTCAACGTTGGTATGTAT+GGG 0.562663 2:-73597741 MsaT010455.1:CDS
CAAAGATCAAGGGATGTGAA+TGG 0.572234 2:-73596255 MsaT010455.1:CDS
AATGTCAAGGACTCGATAAG+TGG 0.572588 2:-73596342 MsaT010455.1:CDS
AAGTATCACAGTGCATTGGT+TGG 0.580288 2:-73596314 MsaT010455.1:CDS
TACCCATTGATTCCATTCGA+AGG 0.580537 2:+73596451 None:intergenic
AAGAGGCGCCGTGGACATTG+TGG 0.583174 2:-73596033 MsaT010455.1:CDS
CATTTGATTTCCATACTCGA+TGG 0.584898 2:+73596480 None:intergenic
CAATAAAATCATCATCGACA+AGG 0.586633 2:+73597693 None:intergenic
TCAAAAGTATCACAGTGCAT+TGG 0.590616 2:-73596318 MsaT010455.1:CDS
GAAGCATGGCATACAATCTG+AGG 0.592856 2:-73596157 MsaT010455.1:CDS
GTGAGGCAAGAGCTGACACT+AGG 0.593063 2:-73597805 MsaT010455.1:CDS
CATCGACAAGGCTATGATTG+TGG 0.600537 2:+73597705 None:intergenic
TAGCAGTGAAAATACCTCAC+AGG 0.603589 2:-73598242 MsaT010455.1:intron
TGTTATGGTACATTTGAACA+TGG 0.622131 2:-73596406 MsaT010455.1:CDS
CAATAAAATCCGTAAGCGTG+AGG 0.624421 2:-73597822 MsaT010455.1:CDS
AGCTTTAAATCAAAGATCAA+GGG 0.625411 2:-73596265 MsaT010455.1:CDS
TTGATTCTGGTAGTCAACGT+TGG 0.631816 2:-73597751 MsaT010455.1:CDS
ATATTTGTTTGCTACAGACA+AGG 0.641722 2:-73597869 MsaT010455.1:CDS
CGTGGACATTGTGGTAAATG+TGG 0.643084 2:-73596024 MsaT010455.1:CDS
GTGACTAAATATTGTAGACA+AGG 0.643993 2:-73596528 MsaT010455.1:CDS
GCAAGAGCTGACACTAGGTG+CGG 0.669601 2:-73597800 MsaT010455.1:CDS
GGATTTCTTCCGGGTCACAG+GGG 0.672070 2:-73597671 MsaT010455.1:CDS
ACAGAAACTATATGTACACA+AGG 0.681807 2:+73596426 None:intergenic
GTGGTGCAGGAACTTCTAGA+CGG 0.688641 2:-73596062 MsaT010455.1:CDS
TGGTTTGGCATGCAAGTCTG+CGG 0.712218 2:-73596235 MsaT010455.1:CDS
AGAAAGGCATGATACCTGTG+AGG 0.736671 2:+73598228 None:intergenic
CTATCAAGAACCAAACACGA+TGG 0.739845 2:+73596375 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 73595900 73598296 73595900 ID=MsaG010455
Chr2 mRNA 73595900 73598296 73595900 ID=MsaT010455.1;Parent=MsaG010455
Chr2 exon 73595900 73596606 73595900 ID=MsaT010455.1.exon3;Parent=MsaT010455.1
Chr2 CDS 73595900 73596606 73595900 ID=cds.MsaT010455.1;Parent=MsaT010455.1
Chr2 exon 73597624 73598131 73597624 ID=MsaT010455.1.exon2;Parent=MsaT010455.1
Chr2 CDS 73597624 73598131 73597624 ID=cds.MsaT010455.1;Parent=MsaT010455.1
Chr2 exon 73598243 73598296 73598243 ID=MsaT010455.1.exon1;Parent=MsaT010455.1
Chr2 CDS 73598243 73598296 73598243 ID=cds.MsaT010455.1;Parent=MsaT010455.1
Gene Sequence

>MsaT010455.1

ATGGCTAACAGTAACGATGAACAGATTCTGAGTAGCAGTGAAAATACCTCACAGTTTATGGAGTTTGGAGATATAGAAAGTGATATGAAAGTTGGATTTGGATTTGCTATTGAAGGAATGGAAGATATAGGGAAAATTGATTTTAAAAAATTAAATGCAAGTGATGTTATGAAGTTTCATTTTCCAAATATTGCAGTGGCTTATACATTTTACAATTGGTATGCTCGAATGAATGGTTTTTCTGCTCGTAGAAGCAAAGTAAGGAGAAACAAAAACAACGAGATTATACAACAAATATTTGTTTGCTACAGACAAGGTTTCAGAGAAAAAAAGCTTGAGAACAATAAAATCCGTAAGCGTGAGGCAAGAGCTGACACTAGGTGCGGATGTGATGCCAAATGTAGTGTGCACATTGATTCTGGTAGTCAACGTTGGTATGTATGGGATTTTAACAATGACCACAATCATAGCCTTGTCGATGATGATTTTATTGGATTTCTTCCGGGTCACAGGGGGATGGATGATGATGACATTCTCCAAATGAAAAATCTTAGAAAGTCTGCTTCTGCTGTCACAGTGAAGGTCAACAAGCAAACAACTAATTGTTTCGAATATTCTGTGACTAAATATTGTAGACAAGGAAGTGAATGGAATGTTTCTTACTGGCCATCGAGTATGGAAATCAAATGTTCCTGCCTTCGAATGGAATCAATGGGTATTCCTTGTGTACATATAGTTTCTGTTATGGTACATTTGAACATGGTTGAAATACCATCGTGTTTGGTTCTTGATAGATGGTCAAAAAATGTCAAGGACTCGATAAGTGGTTCAAAAGTATCACAGTGCATTGGTTGGGATTCAATGACAGTGTGTCGATATAAAGCTTTAAATCAAAGATCAAGGGATGTGAATGGTTTGGCATGCAAGTCTGCGGAAGATTATCTGGAACTGATGAATCTGTTGAATGACCATTATGAGAGTCTTAAAAAGAAGCATGGCATACAATCTGAGGATGTATCCAATGTTGCAAACAGAACCGAACAATTTATTCAAGATCCAAAACGTGCAAAAACTAAGGGGCGTGGTGGTGCAGGAACTTCTAGACGGAAAAATAAGAGGCGCCGTGGACATTGTGGTAAATGTGGAATTGTTGGACATAATAGACAAACTTGCACAACATTGAAAAAAGTAAATGGAGAAGTTGATGATGAATATGAAACAACTAGTCATGTATCTGATGAAGATTGGTACTCTGAAGATGATGAATAA
Protein sequence

>MsaT010455.1

MANSNDEQILSSSENTSQFMEFGDIESDMKVGFGFAIEGMEDIGKIDFKKLNASDVMKFHFPNIAVAYTFYNWYARMNGFSARRSKVRRNKNNEIIQQIFVCYRQGFREKKLENNKIRKREARADTRCGCDAKCSVHIDSGSQRWYVWDFNNDHNHSLVDDDFIGFLPGHRGMDDDDILQMKNLRKSASAVTVKVNKQTTNCFEYSVTKYCRQGSEWNVSYWPSSMEIKCSCLRMESMGIPCVHIVSVMVHLNMVEIPSCLVLDRWSKNVKDSISGSKVSQCIGWDSMTVCRYKALNQRSRDVNGLACKSAEDYLELMNLLNDHYESLKKKHGIQSEDVSNVANRTEQFIQDPKRAKTKGRGGAGTSRRKNKRRRGHCGKCGIVGHNRQTCTTLKKVNGEVDDEYETTSHVSDEDWYSEDDE*