AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010466


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010466 MtrunA17_Chr2g0325521 98.381 247 4 0 1 247 1 247 7.22e-180 493
MsaG010466 MtrunA17_Chr4g0041051 71.875 256 63 3 1 247 1 256 6.45e-121 344
MsaG010466 MtrunA17_Chr4g0045901 44.672 244 124 6 2 235 13 255 4.52e-51 167
MsaG010466 MtrunA17_Chr7g0255961 47.253 182 86 4 54 234 70 242 3.74e-42 144
MsaG010466 MtrunA17_Chr1g0198801 40.594 202 109 3 45 242 144 338 2.16e-34 126
MsaG010466 MtrunA17_Chr3g0113721 33.333 201 115 5 46 234 26 219 7.00e-31 114
MsaG010466 MtrunA17_Chr5g0412371 38.462 143 74 3 54 188 40 176 4.12e-26 101
MsaG010466 MtrunA17_Chr5g0427361 40.606 165 72 2 49 188 27 190 4.73e-25 99.4
MsaG010466 MtrunA17_Chr2g0283714 51.250 80 36 1 91 170 16 92 3.47e-20 83.6
MsaG010466 MtrunA17_Chr5g0411341 40.244 82 49 0 28 109 459 540 3.21e-12 65.9
MsaG010466 MtrunA17_Chr8g0366751 39.506 81 49 0 29 109 429 509 8.35e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010466 AT1G68810.1 41.327 196 107 5 45 236 160 351 2.27e-33 124
MsaG010466 AT3G25710.1 46.622 148 70 2 45 188 118 260 1.01e-31 119
MsaG010466 AT3G56770.1 38.857 175 98 4 51 223 37 204 1.60e-30 113
MsaG010466 AT2G40200.1 39.634 164 89 3 61 219 65 223 1.99e-29 111
MsaG010466 AT2G41130.1 40.571 175 96 3 51 223 59 227 4.30e-27 105
MsaG010466 AT4G38070.1 36.154 130 76 2 59 188 92 214 1.29e-17 80.1
MsaG010466 AT4G17880.1 40.789 76 45 0 39 114 393 468 2.24e-11 63.5
MsaG010466 AT5G46760.1 44.156 77 42 1 39 114 391 467 2.29e-11 63.5
MsaG010466 AT1G32640.1 36.634 101 61 2 33 130 421 521 3.64e-11 63.2

Find 38 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCTTCTTCACTGGTTT+TGG 0.180837 2:+73704176 MsaT010466.1:CDS
CACTTCATCTTATGATAATA+AGG 0.194458 2:+73705441 MsaT010466.1:CDS
AATGATGCTCTAATTGAATA+AGG 0.233216 2:-73705365 None:intergenic
CTTATCTCATCACTGTCTTT+TGG 0.234136 2:-73705308 None:intergenic
ATATTCAGTTCTTCATCTTT+AGG 0.245592 2:+73705656 MsaT010466.1:CDS
TCACAAGAAGGTGGATTAAA+TGG 0.279597 2:+73705338 MsaT010466.1:CDS
TTTCAAGGTGTAAATGGAAA+TGG 0.359014 2:+73704147 MsaT010466.1:CDS
TGTGGAGGCAGCTGTGAAAT+TGG 0.360925 2:+73704218 MsaT010466.1:CDS
TGTTATCCCAGGTGCTAATA+AGG 0.365921 2:+73704350 MsaT010466.1:CDS
TGTAATAACTTCAGCTAGTA+AGG 0.369694 2:-73705231 None:intergenic
AAGGGCAGAGATTGCAACTT+TGG 0.372459 2:+73705460 MsaT010466.1:CDS
GAGTATCGACAGTTTCTTGC+TGG 0.401116 2:+73705545 MsaT010466.1:CDS
CAAAGCTTCAATACTTCTCT+CGG 0.423960 2:-73704259 None:intergenic
AGGGCAGAGATTGCAACTTT+GGG 0.424505 2:+73705461 MsaT010466.1:CDS
TCTTATATCAGACAATAAAC+CGG 0.424693 2:-73705405 None:intergenic
GGTGTAAATGGAAATGGATA+TGG 0.430429 2:+73704153 MsaT010466.1:CDS
ATGATGCTCTAATTGAATAA+GGG 0.448376 2:-73705364 None:intergenic
ATATGGTTCATCTTCTTCAC+TGG 0.451383 2:+73704170 MsaT010466.1:CDS
ACTTCATCTTATGATAATAA+GGG 0.489015 2:+73705442 MsaT010466.1:CDS
TCGACAGTTTCTTGCTGGAT+CGG 0.489881 2:+73705550 MsaT010466.1:CDS
CTTATATCAGACAATAAACC+GGG 0.504302 2:-73705404 None:intergenic
GAACTAACCTTATTAGCACC+TGG 0.521161 2:-73704357 None:intergenic
AAAGCTTCAATACTTCTCTC+GGG 0.540932 2:-73704258 None:intergenic
AGCAAGAAACTGTCGATACT+CGG 0.542573 2:-73705543 None:intergenic
TTATGTTGTGAATACAAACC+CGG 0.569012 2:+73705386 MsaT010466.1:CDS
AACTAACCTTATTAGCACCT+GGG 0.573545 2:-73704356 None:intergenic
AATGCAGCACAAGCAAGTGA+AGG 0.583141 2:+73705275 MsaT010466.1:CDS
GCTTCAAGTAGAATGAATGA+TGG 0.589941 2:+73704120 MsaT010466.1:CDS
GCAGAGATTGCAACTTTGGG+AGG 0.592208 2:+73705464 MsaT010466.1:CDS
ATGCAGCACAAGCAAGTGAA+GGG 0.603381 2:+73705276 MsaT010466.1:CDS
ATCATAGTGAAGCAGAGAGG+AGG 0.603979 2:+73704286 MsaT010466.1:CDS
TCTTAGAAACTAGAAAGAGA+AGG 0.613686 2:+73705624 MsaT010466.1:CDS
TAGAAACTAGAAAGAGAAGG+AGG 0.614835 2:+73705627 MsaT010466.1:CDS
ATAAGAGTTGAATCACAAGA+AGG 0.621854 2:+73705326 MsaT010466.1:CDS
ACACTTCGTAGTGTTATCCC+AGG 0.622939 2:+73704339 MsaT010466.1:CDS
AGAGTTGAATCACAAGAAGG+TGG 0.624818 2:+73705329 MsaT010466.1:CDS
GCTGTGAAATTGGAACGCAA+AGG 0.704659 2:+73704228 MsaT010466.1:CDS
AGAATCATAGTGAAGCAGAG+AGG 0.708784 2:+73704283 MsaT010466.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 73704102 73705691 73704102 ID=MsaG010466
Chr2 mRNA 73704102 73705691 73704102 ID=MsaT010466.1;Parent=MsaG010466
Chr2 exon 73704102 73704371 73704102 ID=MsaT010466.1.exon1;Parent=MsaT010466.1
Chr2 CDS 73704102 73704371 73704102 ID=cds.MsaT010466.1;Parent=MsaT010466.1
Chr2 exon 73705218 73705691 73705218 ID=MsaT010466.1.exon2;Parent=MsaT010466.1
Chr2 CDS 73705218 73705691 73705218 ID=cds.MsaT010466.1;Parent=MsaT010466.1
Gene Sequence

>MsaT010466.1

ATGGAAATGGATGATTTTGCTTCAAGTAGAATGAATGATGGTTGTTTTCAAGGTGTAAATGGAAATGGATATGGTTCATCTTCTTCACTGGTTTTGGATAGAGAAAAAGGAGAGCTTGTGGAGGCAGCTGTGAAATTGGAACGCAAAGGTGTGTCGCCCGAGAGAAGTATTGAAGCTTTGAAGAATCATAGTGAAGCAGAGAGGAGGAGAAGAGCAAGAATTAATTCACATCTTGATACACTTCGTAGTGTTATCCCAGGTGCTAATAAGTTGGACAAAGCATCCTTACTAGCTGAAGTTATTACACATTTGAAAGAGCTGAAAACAAATGCAGCACAAGCAAGTGAAGGGTTCATTATTCCAAAAGACAGTGATGAGATAAGAGTTGAATCACAAGAAGGTGGATTAAATGGTTACCCTTATTCAATTAGAGCATCATTATGTTGTGAATACAAACCCGGTTTATTGTCTGATATAAGAAAAGCACTTGATGCACTTCATCTTATGATAATAAGGGCAGAGATTGCAACTTTGGGAGGTAGAATGAAGAATGTCTTTGTCATAATTAGCTGCAAAGAACAGAATTTTGAAGATGCCGAGTATCGACAGTTTCTTGCTGGATCGGTTCATCAAGCTCTTAGATCTGTACTTGATAGATTTTCTGTTTCACAAGATATCTTAGAAACTAGAAAGAGAAGGAGGATTTCTATATTCAGTTCTTCATCTTTAGGTGATTTCTTATAA

Protein sequence

>MsaT010466.1

MEMDDFASSRMNDGCFQGVNGNGYGSSSSLVLDREKGELVEAAVKLERKGVSPERSIEALKNHSEAERRRRARINSHLDTLRSVIPGANKLDKASLLAEVITHLKELKTNAAQASEGFIIPKDSDEIRVESQEGGLNGYPYSIRASLCCEYKPGLLSDIRKALDALHLMIIRAEIATLGGRMKNVFVIISCKEQNFEDAEYRQFLAGSVHQALRSVLDRFSVSQDILETRKRRRISIFSSSSLGDFL*