AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010502


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010502 MtrunA17_Chr2g0325131 95.758 330 6 2 1 330 1 322 0.0 638
MsaG010502 MtrunA17_Chr4g0040891 62.931 348 92 14 1 331 1 328 5.14e-125 361
MsaG010502 MtrunA17_Chr2g0316551 53.870 323 116 10 1 302 1 311 2.54e-96 289
MsaG010502 MtrunA17_Chr8g0380611 35.094 265 145 8 44 294 73 324 2.96e-28 112
MsaG010502 MtrunA17_Chr5g0432701 48.936 94 48 0 198 291 158 251 1.93e-20 90.9
MsaG010502 MtrunA17_Chr4g0036071 44.828 116 56 1 197 312 358 465 2.91e-20 91.3
MsaG010502 MtrunA17_Chr2g0329551 44.737 114 58 2 197 310 219 327 2.55e-19 88.2
MsaG010502 MtrunA17_Chr3g0107491 46.237 93 50 0 199 291 177 269 1.40e-18 85.5
MsaG010502 MtrunA17_Chr6g0463571 46.000 100 54 0 197 296 149 248 5.02e-18 83.2
MsaG010502 MtrunA17_Chr3g0128781 45.745 94 51 0 198 291 107 200 1.73e-17 81.6
MsaG010502 MtrunA17_Chr1g0156051 48.980 98 46 2 198 293 386 481 1.26e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010502 AT3G58120.1 59.249 346 109 10 1 331 1 329 8.38e-121 351
MsaG010502 AT2G42380.2 57.225 346 107 8 1 331 1 320 8.09e-115 335
MsaG010502 AT2G42380.1 53.757 346 108 9 1 331 1 309 2.76e-103 305
MsaG010502 AT3G58120.2 58.020 293 96 8 1 278 1 281 6.87e-100 296
MsaG010502 AT2G42380.5 56.701 291 87 7 1 278 1 265 8.47e-93 277
MsaG010502 AT2G42380.3 49.206 252 89 7 1 239 1 226 9.63e-62 197
MsaG010502 AT2G42380.4 49.206 252 89 7 1 239 1 226 9.63e-62 197
MsaG010502 AT1G58110.1 30.448 335 161 11 2 294 20 324 1.81e-27 110
MsaG010502 AT1G58110.2 30.448 335 161 11 2 294 20 324 1.81e-27 110
MsaG010502 AT1G43700.1 46.491 114 56 1 185 293 175 288 5.44e-21 92.4
MsaG010502 AT4G38900.2 48.421 95 49 0 197 291 392 486 5.50e-20 91.3
MsaG010502 AT4G38900.3 48.421 95 49 0 197 291 392 486 5.50e-20 91.3
MsaG010502 AT5G04840.1 29.276 304 163 8 2 296 5 265 2.17e-19 87.4
MsaG010502 AT2G40620.1 46.316 95 51 0 197 291 146 240 3.31e-19 87.8
MsaG010502 AT1G06850.1 48.387 93 48 0 199 291 148 240 1.11e-18 85.9
MsaG010502 AT1G06070.1 46.316 95 51 0 197 291 208 302 1.35e-18 86.7
MsaG010502 AT2G31370.5 31.174 247 143 5 62 291 57 293 4.28e-18 84.7
MsaG010502 AT2G21230.4 46.392 97 52 0 197 293 368 464 4.59e-18 85.5
MsaG010502 AT2G21230.1 46.392 97 52 0 197 293 368 464 4.59e-18 85.5
MsaG010502 AT2G31370.2 31.174 247 143 5 62 291 57 293 4.75e-18 84.7
MsaG010502 AT2G31370.4 31.174 247 143 5 62 291 57 293 4.75e-18 84.7
MsaG010502 AT2G31370.1 31.174 247 143 5 62 291 57 293 4.75e-18 84.7
MsaG010502 AT2G31370.7 31.174 247 143 5 62 291 57 293 4.75e-18 84.7
MsaG010502 AT2G31370.3 31.174 247 143 5 62 291 57 293 4.75e-18 84.7
MsaG010502 AT2G31370.6 31.174 247 143 5 62 291 57 293 4.75e-18 84.7
MsaG010502 AT4G38900.1 45.545 101 49 1 197 291 392 492 8.41e-18 84.7
MsaG010502 AT1G35490.2 28.226 248 133 7 46 291 54 258 5.28e-17 80.5
MsaG010502 AT1G35490.1 28.226 248 133 7 46 291 54 258 5.28e-17 80.5
MsaG010502 AT5G04840.2 29.371 286 150 8 2 278 5 247 4.18e-16 77.0
MsaG010502 AT2G21230.3 42.718 103 53 1 197 293 368 470 1.02e-15 78.2
MsaG010502 AT2G21230.2 46.341 82 44 0 197 278 368 449 3.30e-14 73.6
MsaG010502 AT5G07160.1 41.237 97 57 0 195 291 44 140 2.95e-12 64.3
MsaG010502 AT1G06850.2 68.889 45 14 0 198 242 147 191 5.00e-11 62.4

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGACATGTTTGGGATCTT+AGG 0.259620 2:+74086011 None:intergenic
GTTTGTAGTGACAATGTTAT+TGG 0.263094 2:+74085932 None:intergenic
GCATTAGAATCTTGGCAAAT+AGG 0.275046 2:-74085021 MsaT010502.1:intron
GAGAAGAAGGAAAATGATTT+AGG 0.296863 2:-74085588 MsaT010502.1:CDS
GCCAACTTGGTGACATGTTT+GGG 0.333815 2:+74086003 None:intergenic
CATTGGCTTTCTACCTCATC+AGG 0.351950 2:+74085522 None:intergenic
GCGTAGTGTAACTTCATTGC+AGG 0.377738 2:-74084945 MsaT010502.1:intron
GGCCAACTTGGTGACATGTT+TGG 0.381627 2:+74086002 None:intergenic
GACAATGTTATTGGTTGAAA+TGG 0.385822 2:+74085941 None:intergenic
TCTGTTTGAGAATCACATAT+TGG 0.388915 2:+74083462 None:intergenic
CCATCTTCTTGAGGTTCTGA+TGG 0.391631 2:+74083526 None:intergenic
GAAGAATAAACAAGAGAAGA+AGG 0.396694 2:-74085601 MsaT010502.1:CDS
ACCAATGCTTGATGATTGTC+GGG 0.399558 2:-74085799 MsaT010502.1:CDS
AAGAAGATGGAAAATGCTAA+TGG 0.406779 2:-74083513 MsaT010502.1:CDS
ATCAGAATCAGAACCCTTCA+TGG 0.433073 2:-74085905 MsaT010502.1:CDS
GGAGATAGAAAGATTAAGAC+AGG 0.441141 2:-74083556 MsaT010502.1:CDS
GCATTGTTGTTGTTACTTTG+AGG 0.444336 2:+74085480 None:intergenic
CACCAATGCTTGATGATTGT+CGG 0.462711 2:-74085800 MsaT010502.1:CDS
TTCTGATCTGAAGGACTCGA+AGG 0.485897 2:+74085651 None:intergenic
GGTAGAAAGCCAATGCAAAC+AGG 0.488493 2:-74085514 MsaT010502.1:CDS
CCATCAGAACCTCAAGAAGA+TGG 0.490491 2:-74083526 MsaT010502.1:CDS
ATGTTTAGTGATGAAATATC+TGG 0.505923 2:-74085711 MsaT010502.1:CDS
TGAAAAGCTTGTCTTGTGCA+AGG 0.507196 2:+74084661 None:intergenic
TTATTGGTTGAAATGGATGT+TGG 0.515770 2:+74085948 None:intergenic
TTGACAAAATTCTGATCTGA+AGG 0.516864 2:+74085642 None:intergenic
CCCCGACAATCATCAAGCAT+TGG 0.530465 2:+74085798 None:intergenic
TTTAGGAGAGATGAAGAAAG+AGG 0.538946 2:-74085571 MsaT010502.1:CDS
AGGAACTCATCCACCCATGA+AGG 0.539506 2:+74085891 None:intergenic
GAAAAGCTTGTCTTGTGCAA+GGG 0.539760 2:+74084662 None:intergenic
CTGATCCAAAGAGAGTCAAA+AGG 0.572655 2:-74085434 MsaT010502.1:intron
TTCGATGAAGACAACGTTGG+TGG 0.575587 2:+74085675 None:intergenic
TCCCAAACATGTCACCAAGT+TGG 0.580152 2:-74086004 MsaT010502.1:CDS
AGATATTTCATCACTAAACA+TGG 0.586033 2:+74085713 None:intergenic
GACATGTTTGGGATCTTAGG+AGG 0.588767 2:+74086014 None:intergenic
CTCATCAAGAGGCATTGAAG+AGG 0.590362 2:-74083578 MsaT010502.1:CDS
CAAGGCGAGGTGCTCATCGA+AGG 0.594155 2:-74085854 MsaT010502.1:CDS
TCATCAAGAGGCATTGAAGA+GGG 0.599782 2:-74083577 MsaT010502.1:CDS
GAATCAGAACCCTTCATGGG+TGG 0.607660 2:-74085901 MsaT010502.1:CDS
ATTGAAATATGAACCTGATG+AGG 0.608223 2:-74085535 MsaT010502.1:CDS
TCAGAATCAGAACCCTTCAT+GGG 0.609308 2:-74085904 MsaT010502.1:CDS
TCTGATCTGAAGGACTCGAA+GGG 0.610267 2:+74085652 None:intergenic
TTTACATGCAGCTCATCAAG+AGG 0.610732 2:-74083589 MsaT010502.1:intron
ATGAGCACCTCGCCTTGCCG+AGG 0.614435 2:+74085860 None:intergenic
GGAACTCATCCACCCATGAA+GGG 0.626852 2:+74085892 None:intergenic
CAAATAGGCAATCAGCACAA+AGG 0.647617 2:-74085006 MsaT010502.1:CDS
GCGAGGTGCTCATCGAAGGT+CGG 0.657525 2:-74085850 MsaT010502.1:CDS
CAGCACAAAGGTCACGTGTG+AGG 0.661809 2:-74084994 MsaT010502.1:CDS
GCCTTGCCGAGGAAAAGTCG+AGG 0.688307 2:+74085871 None:intergenic
GATTGTCGGGGTAAACACGA+TGG 0.708511 2:-74085786 MsaT010502.1:CDS
GGGTTCGATGAAGACAACGT+TGG 0.721008 2:+74085672 None:intergenic
CCAATGCTTGATGATTGTCG+GGG 0.745183 2:-74085798 MsaT010502.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 74083437 74086048 74083437 ID=MsaG010502
Chr2 mRNA 74083437 74086048 74083437 ID=MsaT010502.1;Parent=MsaG010502
Chr2 exon 74083437 74083600 74083437 ID=MsaT010502.1.exon4;Parent=MsaT010502.1
Chr2 CDS 74083437 74083600 74083437 ID=cds.MsaT010502.1;Parent=MsaT010502.1
Chr2 exon 74084655 74084781 74084655 ID=MsaT010502.1.exon3;Parent=MsaT010502.1
Chr2 CDS 74084655 74084781 74084655 ID=cds.MsaT010502.1;Parent=MsaT010502.1
Chr2 exon 74084946 74085036 74084946 ID=MsaT010502.1.exon2;Parent=MsaT010502.1
Chr2 CDS 74084946 74085036 74084946 ID=cds.MsaT010502.1;Parent=MsaT010502.1
Chr2 exon 74085435 74086048 74085435 ID=MsaT010502.1.exon1;Parent=MsaT010502.1
Chr2 CDS 74085435 74086048 74085435 ID=cds.MsaT010502.1;Parent=MsaT010502.1
Gene Sequence

>MsaT010502.1

ATGGCACAATTACCTCCTAAGATCCCAAACATGTCACCAAGTTGGCCAGATTTTTCCAAACACCAAAAAAATATTATGCCAACATCCATTTCAACCAATAACATTGTCACTACAAACAATTATCAGAATCAGAACCCTTCATGGGTGGATGAGTTCCTCGACTTTTCCTCGGCAAGGCGAGGTGCTCATCGAAGGTCGGTCAGCGATTCAGTCACATTCTTAGAAACACCAATGCTTGATGATTGTCGGGGTAAACACGATGGTAACAACAATGACAACGAGTTCGACAAGTTTGATGATGAACAATTTTTGTCCATGTTTAGTGATGAAATATCTGGTGTCAATAACATGCCACCAACGTTGTCTTCATCGAACCCTTCGAGTCCTTCAGATCAGAATTTTGTCAATGATGATAATGAGAAAGAGAAGAATAAACAAGAGAAGAAGGAAAATGATTTAGGAGAGATGAAGAAAGAGGAAGATGAGAAGCAATTGAAATATGAACCTGATGAGGTAGAAAGCCAATGCAAACAGGAAAATTCACAGCCTCAAAGTAACAACAACAATGCAACTTCTTCAAATGATAGAATTACTGATCCAAAGAGAGTCAAAAGAATCTTGGCAAATAGGCAATCAGCACAAAGGTCACGTGTGAGGAAGCTGCAATACATATCAGAGCTTGAGCGTAGTGTAACTTCATTGCAGGCTGAAGTTTCAGTGCTATCTCCAAGAGTTGCATTTTTGGATCATCAACGATTGCTTCTAAATGTTGACAACAGTGCTCTCAAGCAAAGAATTGCAGCCCTTGCACAAGACAAGCTTTTCAAAGATGCTCATCAAGAGGCATTGAAGAGGGAGATAGAAAGATTAAGACAGGTATATCACCATCAGAACCTCAAGAAGATGGAAAATGCTAATGGATCACCCTTACCATCCCCATCACAAAAGCCAATATGTGATTCTCAAACAGAAAGCGAACAACTACTCAATGTATGA

Protein sequence

>MsaT010502.1

MAQLPPKIPNMSPSWPDFSKHQKNIMPTSISTNNIVTTNNYQNQNPSWVDEFLDFSSARRGAHRRSVSDSVTFLETPMLDDCRGKHDGNNNDNEFDKFDDEQFLSMFSDEISGVNNMPPTLSSSNPSSPSDQNFVNDDNEKEKNKQEKKENDLGEMKKEEDEKQLKYEPDEVESQCKQENSQPQSNNNNATSSNDRITDPKRVKRILANRQSAQRSRVRKLQYISELERSVTSLQAEVSVLSPRVAFLDHQRLLLNVDNSALKQRIAALAQDKLFKDAHQEALKREIERLRQVYHHQNLKKMENANGSPLPSPSQKPICDSQTESEQLLNV*