AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010513

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010513 MtrunA17_Chr4g0040571 64.138 435 108 19 1 398 63 486 1.52e-166 476
MsaG010513 MtrunA17_Chr2g0324981 86.260 262 21 1 153 414 1 247 6.87e-150 424
MsaG010513 MtrunA17_Chr4g0029521 49.080 326 121 6 1 321 62 347 7.19e-98 301
MsaG010513 MtrunA17_Chr1g0181381 43.478 460 177 15 1 411 62 487 3.15e-97 299
MsaG010513 MtrunA17_Chr7g0268961 50.617 324 110 6 1 323 46 320 5.21e-96 296
MsaG010513 MtrunA17_Chr2g0330171 89.209 139 15 0 1 139 70 208 1.22e-93 289
MsaG010513 MtrunA17_Chr1g0162971 49.231 325 134 7 1 318 65 365 3.09e-93 287
MsaG010513 MtrunA17_Chr8g0342421 89.928 139 14 0 1 139 86 224 6.78e-93 289
MsaG010513 MtrunA17_Chr7g0263941 64.390 205 66 2 1 205 58 255 1.46e-90 279
MsaG010513 MtrunA17_Chr1g0210821 48.397 343 154 7 1 321 68 409 9.06e-90 282
MsaG010513 MtrunA17_Chr1g0185121 50.156 321 104 8 1 320 64 329 1.43e-89 278
MsaG010513 MtrunA17_Chr1g0163001 84.892 139 21 0 1 139 67 205 1.76e-88 278
MsaG010513 MtrunA17_Chr1g0199101 84.058 138 22 0 1 138 71 208 4.21e-88 277
MsaG010513 MtrunA17_Chr2g0324991 100.000 118 0 0 1 118 16 133 3.01e-87 261
MsaG010513 MtrunA17_Chr1g0151351 81.818 143 25 1 1 142 65 207 7.73e-85 266
MsaG010513 MtrunA17_Chr2g0326381 69.591 171 42 3 1 170 56 217 4.16e-84 263
MsaG010513 MtrunA17_Chr3g0141431 76.866 134 30 1 1 133 46 179 1.38e-77 246
MsaG010513 MtrunA17_Chr6g0479921 62.879 132 48 1 1 131 53 184 1.55e-57 195
MsaG010513 MtrunA17_Chr8g0375521 61.364 132 49 2 1 130 51 182 2.02e-55 188
MsaG010513 MtrunA17_Chr1g0200421 34.752 141 80 3 1 129 246 386 5.22e-20 92.0
MsaG010513 MtrunA17_Chr3g0123941 35.036 137 81 3 1 129 132 268 1.63e-19 89.0
MsaG010513 MtrunA17_Chr2g0320341 35.000 140 77 3 1 126 239 378 2.59e-19 89.4
MsaG010513 MtrunA17_Chr4g0053341 36.957 138 77 5 1 129 82 218 5.35e-19 85.5
MsaG010513 MtrunA17_Chr5g0422571 36.810 163 87 8 1 152 109 266 6.25e-19 86.7
MsaG010513 MtrunA17_Chr8g0352011 36.232 138 78 5 1 129 174 310 6.79e-18 84.3
MsaG010513 MtrunA17_Chr8g0382121 36.496 137 76 5 1 126 120 256 7.06e-18 84.0
MsaG010513 MtrunA17_Chr7g0258011 33.766 154 82 7 1 136 184 335 2.87e-17 82.4
MsaG010513 MtrunA17_Chr1g0173841 34.783 138 80 5 1 129 223 359 1.10e-16 81.3
MsaG010513 MtrunA17_Chr2g0321291 36.567 134 75 5 5 129 145 277 1.46e-16 80.1
MsaG010513 MtrunA17_Chr1g0198651 33.557 149 86 5 1 137 110 257 1.51e-16 79.7
MsaG010513 MtrunA17_Chr2g0328921 34.058 138 81 5 1 129 146 282 1.56e-16 80.1
MsaG010513 MtrunA17_Chr4g0026521 35.821 134 76 5 5 129 130 262 1.24e-15 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010513 AT1G03840.2 49.661 443 163 13 1 398 63 490 5.90e-119 355
MsaG010513 AT1G03840.1 49.661 443 163 13 1 398 65 492 8.04e-119 355
MsaG010513 AT5G44160.2 50.000 438 133 14 1 398 22 413 5.62e-117 348
MsaG010513 AT5G44160.1 50.000 438 133 14 1 398 61 452 9.19e-117 349
MsaG010513 AT2G02080.6 46.705 349 140 10 1 327 1 325 3.22e-92 285
MsaG010513 AT2G02080.2 46.705 349 140 10 1 327 1 325 3.22e-92 285
MsaG010513 AT5G66730.1 87.050 139 18 0 1 139 56 194 5.78e-92 286
MsaG010513 AT2G02080.1 46.991 349 139 10 1 327 78 402 6.43e-92 286
MsaG010513 AT2G02080.4 46.991 349 139 10 1 327 78 402 6.43e-92 286
MsaG010513 AT2G02080.5 46.991 349 139 10 1 327 78 402 6.43e-92 286
MsaG010513 AT2G02080.3 46.991 349 139 10 1 327 78 402 6.43e-92 286
MsaG010513 AT4G02670.2 80.405 148 28 1 1 147 79 226 8.05e-90 278
MsaG010513 AT4G02670.1 80.405 148 28 1 1 147 77 224 9.10e-90 277
MsaG010513 AT1G55110.3 85.714 140 19 1 1 139 87 226 4.14e-89 277
MsaG010513 AT1G55110.1 85.714 140 19 1 1 139 87 226 4.14e-89 277
MsaG010513 AT1G55110.2 85.714 140 19 1 1 139 87 226 4.14e-89 277
MsaG010513 AT3G45260.2 43.521 409 161 14 1 398 63 412 5.72e-89 277
MsaG010513 AT3G45260.1 43.521 409 161 14 1 398 63 412 5.72e-89 277
MsaG010513 AT3G13810.6 87.857 140 16 1 1 139 75 214 4.27e-88 276
MsaG010513 AT3G13810.4 87.857 140 16 1 1 139 94 233 5.69e-88 276
MsaG010513 AT3G13810.1 87.857 140 16 1 1 139 94 233 5.69e-88 276
MsaG010513 AT3G13810.5 87.857 140 16 1 1 139 89 228 5.91e-88 276
MsaG010513 AT3G13810.3 87.857 140 16 1 1 139 81 220 8.23e-88 275
MsaG010513 AT3G13810.2 87.857 140 16 1 1 139 95 234 1.17e-87 275
MsaG010513 AT2G02070.2 81.295 139 26 0 1 139 76 214 9.61e-87 276
MsaG010513 AT2G02070.1 81.295 139 26 0 1 139 76 214 9.61e-87 276
MsaG010513 AT1G14580.1 80.576 139 27 0 1 139 77 215 2.80e-86 270
MsaG010513 AT1G14580.2 80.576 139 27 0 1 139 77 215 2.80e-86 270
MsaG010513 AT1G14580.3 80.576 139 27 0 1 139 91 229 4.68e-86 270
MsaG010513 AT5G60470.1 75.177 141 34 1 1 140 59 199 9.82e-81 256
MsaG010513 AT5G60470.2 75.177 141 34 1 1 140 68 208 1.68e-80 255
MsaG010513 AT5G60470.3 75.177 141 34 1 1 140 64 204 1.80e-80 255
MsaG010513 AT1G68130.1 65.152 132 44 2 1 130 65 196 4.66e-58 196
MsaG010513 AT1G25250.1 63.910 133 45 2 1 130 34 166 3.20e-57 192
MsaG010513 AT1G25250.3 63.910 133 45 2 1 130 57 189 3.57e-57 192
MsaG010513 AT2G01940.1 62.687 134 48 2 1 132 68 201 1.27e-56 192
MsaG010513 AT2G01940.3 61.481 135 49 3 1 132 68 202 9.06e-53 182
MsaG010513 AT1G68130.2 64.545 110 37 2 23 130 1 110 6.21e-45 159
MsaG010513 AT1G25250.2 63.063 111 38 2 23 130 1 111 1.06e-43 155
MsaG010513 AT2G01940.2 62.500 112 40 2 23 132 1 112 1.34e-43 156
MsaG010513 AT5G22890.1 35.000 140 77 4 1 126 212 351 1.36e-19 90.1
MsaG010513 AT1G34370.2 34.965 143 79 3 1 129 239 381 3.35e-19 90.1
MsaG010513 AT1G34370.1 34.965 143 79 3 1 129 239 381 3.35e-19 90.1
MsaG010513 AT1G34370.3 34.965 143 79 3 1 129 29 171 3.46e-19 87.8
MsaG010513 AT1G13290.1 34.337 166 87 9 1 154 96 251 6.88e-18 84.3
MsaG010513 AT1G34790.1 35.507 138 79 5 1 129 140 276 1.05e-17 83.6
MsaG010513 AT3G20880.1 34.783 138 80 5 1 129 251 387 2.70e-17 83.6
MsaG010513 AT1G51220.1 33.333 138 82 5 1 129 173 309 6.08e-17 82.0
MsaG010513 AT1G08290.1 37.500 136 72 6 5 129 179 312 2.25e-16 80.1
MsaG010513 AT3G57670.1 33.813 139 80 6 1 129 212 348 3.23e-16 80.1

Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGATCAGGTTTATCTCTT+TGG 0.192108 2:+74228008 MsaT010513.1:CDS
GAAGAAACAGCTAGAATAAA+TGG 0.212216 2:+74227827 MsaT010513.1:CDS
TGTATAGTGCAAATGAAAAT+TGG 0.226846 2:+74228122 MsaT010513.1:CDS
CAATCATCCTTCAAGGGCTT+TGG 0.260521 2:+74226850 MsaT010513.1:CDS
AATATAAACAACTCATTATT+AGG 0.262088 2:+74227857 MsaT010513.1:CDS
AGGGCTTTGGGAGACTTAAC+TGG 0.275104 2:+74226863 MsaT010513.1:CDS
CTCTTGACATCCATTATTAG+AGG 0.287901 2:-74228164 None:intergenic
AATAAATTCTCCTCTAATAA+TGG 0.294626 2:+74228154 MsaT010513.1:CDS
GATCTAGATGTTTGATTAGT+TGG 0.313962 2:-74227992 None:intergenic
TAACATCGATAATAATAATA+AGG 0.320786 2:+74228222 MsaT010513.1:CDS
CTGCATGCGACGTCGTTTCA+TGG 0.334137 2:-74228548 None:intergenic
TCGATGTTATTATTAACTAA+TGG 0.340481 2:-74228208 None:intergenic
AAAGAACAAGTAAAGAAATA+AGG 0.347748 2:+74226789 MsaT010513.1:CDS
TGCAGAATGAAGAGAGCGTT+TGG 0.365539 2:+74228566 MsaT010513.1:CDS
TCAGCAGAGAGCTACATTTC+AGG 0.365625 2:+74228505 MsaT010513.1:CDS
GCAAATGAAAATTGGGTGTT+TGG 0.367718 2:+74228130 MsaT010513.1:CDS
GTGTGTGAAATTTGTCTAAA+GGG 0.373283 2:+74226701 MsaT010513.1:CDS
TCATTATTCCTTGGAAGCTT+AGG 0.378657 2:+74228391 MsaT010513.1:CDS
TCTTGATTTGTGCATGAGAT+TGG 0.386882 2:-74227968 None:intergenic
AATCATCCTTCAAGGGCTTT+GGG 0.387364 2:+74226851 MsaT010513.1:CDS
GTACTTGCAACAAGTGATCT+TGG 0.393658 2:+74228481 MsaT010513.1:CDS
TTTGATGATGAGATTGTGAT+TGG 0.412049 2:-74228297 None:intergenic
GCAGAATGAAGAGAGCGTTT+GGG 0.418512 2:+74228567 MsaT010513.1:CDS
GACAAATTTCACACACGAAT+CGG 0.419935 2:-74226694 None:intergenic
CGTGTGTGAAATTTGTCTAA+AGG 0.423777 2:+74226700 MsaT010513.1:CDS
TCTAGCTGTTTCTTCTGCTA+AGG 0.433712 2:-74227819 None:intergenic
TGCATGCGACGTCGTTTCAT+GGG 0.435103 2:-74228547 None:intergenic
GTGTTCACAATCATCCTTCA+AGG 0.445387 2:+74226843 MsaT010513.1:CDS
TTTATGCTGTTCAATCGGAT+TGG 0.446458 2:+74226951 MsaT010513.1:CDS
TCAACTGAATCATTATTCCT+TGG 0.446740 2:+74228382 MsaT010513.1:CDS
AACAACTCATTATTAGGAAT+GGG 0.451735 2:+74227863 MsaT010513.1:CDS
AAGTCTCCCAAAGCCCTTGA+AGG 0.467713 2:-74226857 None:intergenic
GACGTGGACATAACCTTCCT+TGG 0.468137 2:+74226756 MsaT010513.1:CDS
GAAGAGAGTGTATGTGTGTC+CGG 0.471483 2:+74226811 MsaT010513.1:CDS
AAACAACTCATTATTAGGAA+TGG 0.474295 2:+74227862 MsaT010513.1:CDS
GTTCTTTGTTTCAGCTTCCA+AGG 0.486730 2:-74226773 None:intergenic
AGAGCGTTTGGGGACAAACT+AGG 0.488786 2:+74228578 MsaT010513.1:CDS
ATGCATGAGATTCATCAACT+TGG 0.490859 2:+74228076 MsaT010513.1:CDS
TACTTGCAACAAGTGATCTT+GGG 0.493836 2:+74228482 MsaT010513.1:CDS
GTATAGTGCAAATGAAAATT+GGG 0.498164 2:+74228123 MsaT010513.1:CDS
AATAGTCCAAGTCAAGATCA+TGG 0.501314 2:+74228424 MsaT010513.1:CDS
GGAAGCACGGTGAGAAGAAG+TGG 0.504824 2:+74226906 MsaT010513.1:CDS
GAGCGTTTGGGGACAAACTA+GGG 0.510953 2:+74228579 MsaT010513.1:CDS
TTTGTTTCAGCTTCCAAGGA+AGG 0.514000 2:-74226769 None:intergenic
TTATTCCCATGATCTTGACT+TGG 0.518278 2:-74228430 None:intergenic
GGGATAGCTTTGTCACTCAC+AGG 0.519723 2:+74227783 MsaT010513.1:CDS
ACTAATCAAACATCTAGATC+AGG 0.520793 2:+74227995 MsaT010513.1:CDS
AATTCTCTTGTCCCAAACAT+AGG 0.523465 2:+74227920 MsaT010513.1:CDS
CTTGGGTCCGCTACAAGCAC+AGG 0.540913 2:+74228094 MsaT010513.1:CDS
GATTGATGAACTGTGGCAAA+TGG 0.542982 2:-74228627 None:intergenic
ATCTCAAACCTAAGCTTCCA+AGG 0.557018 2:-74228399 None:intergenic
TTGTGAACACATGTCTTCTC+CGG 0.557862 2:-74226830 None:intergenic
GCATGCGACGTCGTTTCATG+GGG 0.563017 2:-74228546 None:intergenic
TGTTGCTGACATGTTTGCTG+AGG 0.569109 2:-74228323 None:intergenic
TGCATGAGATTCATCAACTT+GGG 0.577843 2:+74228077 MsaT010513.1:CDS
ATAGTCCAAGTCAAGATCAT+GGG 0.586895 2:+74228425 MsaT010513.1:CDS
GGAATGGGAGTTAACAACAT+TGG 0.588635 2:+74227878 MsaT010513.1:CDS
TGCCACAGTTCATCAATCAA+CGG 0.590617 2:+74228632 MsaT010513.1:CDS
TGTTCACAATCATCCTTCAA+GGG 0.591498 2:+74226844 MsaT010513.1:CDS
GGGCTTTGGGAGACTTAACT+GGG 0.593735 2:+74226864 MsaT010513.1:CDS
CAGAATGAAGAGAGCGTTTG+GGG 0.617026 2:+74228568 MsaT010513.1:CDS
CATTGCTCCTGTGCTTGTAG+CGG 0.619229 2:-74228101 None:intergenic
ACAGTTCATCAATCAACGGA+TGG 0.619902 2:+74228636 MsaT010513.1:CDS
ATGCGACGTCGTTTCATGGG+GGG 0.625847 2:-74228544 None:intergenic
CATGCGACGTCGTTTCATGG+GGG 0.652534 2:-74228545 None:intergenic
TTGGTGTTGTGAGCTATACA+AGG 0.662064 2:-74228278 None:intergenic
AAAGCTCACTCAAAGACTTG+TGG 0.665203 2:+74226974 MsaT010513.1:CDS
TGTTGTGAGCTATACAAGGA+AGG 0.666542 2:-74228274 None:intergenic
GGATAGCTTTGTCACTCACA+GGG 0.679845 2:+74227784 MsaT010513.1:CDS
AGAGAATACAAATGTGACTG+TGG 0.689229 2:+74227001 MsaT010513.1:CDS
AATCTTCAACTTCATCGACG+TGG 0.715188 2:+74226740 MsaT010513.1:CDS
ATCCGTTGATTGATGAACTG+TGG 0.722662 2:-74228634 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 74226683 74228669 74226683 ID=MsaG010513
Chr2 mRNA 74226683 74228669 74226683 ID=MsaT010513.1;Parent=MsaG010513
Chr2 exon 74226683 74227038 74226683 ID=MsaT010513.1.exon1;Parent=MsaT010513.1
Chr2 CDS 74226683 74227038 74226683 ID=cds.MsaT010513.1;Parent=MsaT010513.1
Chr2 exon 74227781 74228669 74227781 ID=MsaT010513.1.exon2;Parent=MsaT010513.1
Chr2 CDS 74227781 74228669 74227781 ID=cds.MsaT010513.1;Parent=MsaT010513.1
Gene Sequence

>MsaT010513.1

ATGGCAACAAACCGATTCGTGTGTGAAATTTGTCTAAAGGGTTTTCAAAGGGATCAAAATCTTCAACTTCATCGACGTGGACATAACCTTCCTTGGAAGCTGAAACAAAGAACAAGTAAAGAAATAAGGAAGAGAGTGTATGTGTGTCCGGAGAAGACATGTGTTCACAATCATCCTTCAAGGGCTTTGGGAGACTTAACTGGGATAAAGAAGCACTTTTGTAGGAAGCACGGTGAGAAGAAGTGGAAGTGTGAGAAGTGCTCAAAATTTTATGCTGTTCAATCGGATTGGAAAGCTCACTCAAAGACTTGTGGTACTAGAGAATACAAATGTGACTGTGGCACTATCTTTTCAAGGAGGGATAGCTTTGTCACTCACAGGGCATTCTGTGATGCCTTAGCAGAAGAAACAGCTAGAATAAATGGATCAGCAAATATAAACAACTCATTATTAGGAATGGGAGTTAACAACATTGGTAATTACAACATCATGCAAAATTCTCTTGTCCCAAACATAGGAACTCATTTTTCTTCAATTTTCAAACCAATCTCATGCACAAATCAAGAACCAACTAATCAAACATCTAGATCAGGTTTATCTCTTTGGATGTCTCAAACATCTCATGACACAATATTGACTAATAATAGCAATATGCATGAGATTCATCAACTTGGGTCCGCTACAAGCACAGGAGCAATGTATAGTGCAAATGAAAATTGGGTGTTTGGAAATAAATTCTCCTCTAATAATGGATGTCAAGAGTTAACTAGTTCTGTTTCACTTCCATTAGTTAATAATAACATCGATAATAATAATAAGGATAGTACTACAAACAATGTTGTTAGTGTTCCTTCCTTGTATAGCTCACAACACCAATCACAATCTCATCATCAAACAACCTCAGCAAACATGTCAGCAACAGCTTTGTTGCAAAAAGCTGCTCAAATTGGGACAACTTCAACTGAATCATTATTCCTTGGAAGCTTAGGTTTGAGATGCAATAGTCCAAGTCAAGATCATGGGAATAAATTTTGTGGGGTGTATGGTTCAAGTTCAGTACTTGCAACAAGTGATCTTGGGTCAGCAGAGAGCTACATTTCAGGTGAATTGTCACAAATGCCCCCCATGAAACGACGTCGCATGCAGAATGAAGAGAGCGTTTGGGGACAAACTAGGGATTTTCTTGGTGTTGGTGGTGCTAACCATTTGCCACAGTTCATCAATCAACGGATGGATTTGATTTGA
Protein sequence

>MsaT010513.1

MATNRFVCEICLKGFQRDQNLQLHRRGHNLPWKLKQRTSKEIRKRVYVCPEKTCVHNHPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKFYAVQSDWKAHSKTCGTREYKCDCGTIFSRRDSFVTHRAFCDALAEETARINGSANINNSLLGMGVNNIGNYNIMQNSLVPNIGTHFSSIFKPISCTNQEPTNQTSRSGLSLWMSQTSHDTILTNNSNMHEIHQLGSATSTGAMYSANENWVFGNKFSSNNGCQELTSSVSLPLVNNNIDNNNKDSTTNNVVSVPSLYSSQHQSQSHHQTTSANMSATALLQKAAQIGTTSTESLFLGSLGLRCNSPSQDHGNKFCGVYGSSSVLATSDLGSAESYISGELSQMPPMKRRRMQNEESVWGQTRDFLGVGGANHLPQFINQRMDLI*