AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010532

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010532 MtrunA17_Chr2g0324781 96.411 418 6 4 1 412 1 415 0.0 822
MsaG010532 MtrunA17_Chr4g0040391 64.615 195 36 4 31 223 29 192 5.05e-81 251
MsaG010532 MtrunA17_Chr6g0487481 67.883 137 32 2 56 180 70 206 3.75e-66 215
MsaG010532 MtrunA17_Chr8g0389561 70.400 125 29 1 63 179 59 183 4.49e-63 207
MsaG010532 MtrunA17_Chr7g0257831 68.966 116 36 0 73 188 260 375 2.16e-57 200
MsaG010532 MtrunA17_Chr3g0088521 46.522 230 94 6 73 291 260 471 1.14e-56 198
MsaG010532 MtrunA17_Chr3g0135351 55.769 156 64 3 30 180 2 157 1.06e-54 182
MsaG010532 MtrunA17_Chr1g0171151 64.348 115 41 0 73 187 240 354 8.36e-52 183
MsaG010532 MtrunA17_Chr5g0437251 64.957 117 39 1 73 189 213 327 3.34e-51 182
MsaG010532 MtrunA17_Chr5g0430211 61.207 116 45 0 73 188 226 341 1.06e-50 181
MsaG010532 MtrunA17_Chr1g0187591 61.207 116 45 0 73 188 225 340 2.72e-49 177
MsaG010532 MtrunA17_Chr3g0092221 61.207 116 45 0 73 188 154 269 1.30e-48 170
MsaG010532 MtrunA17_Chr3g0105141 56.140 114 49 1 69 181 28 141 2.50e-45 154
MsaG010532 MtrunA17_Chr3g0113651 56.034 116 51 0 73 188 159 274 6.17e-45 162
MsaG010532 MtrunA17_Chr3g0092171 59.813 107 43 0 73 179 271 377 6.31e-43 157
MsaG010532 MtrunA17_Chr3g0105121 50.877 114 55 1 76 188 8 121 8.58e-37 137
MsaG010532 MtrunA17_Chr3g0105131 50.893 112 41 1 70 181 173 270 2.47e-33 130
MsaG010532 MtrunA17_Chr3g0105101 62.162 74 28 0 108 181 195 268 7.84e-30 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010532 AT1G03790.1 46.154 364 136 12 66 411 71 392 3.42e-85 265
MsaG010532 AT5G44260.1 42.569 397 181 12 30 411 16 380 3.63e-78 247
MsaG010532 AT2G19810.1 70.803 137 30 1 56 182 47 183 1.48e-70 226
MsaG010532 AT4G29190.1 35.181 415 178 13 24 412 7 356 2.14e-68 221
MsaG010532 AT2G25900.2 74.561 114 29 0 71 184 84 197 3.38e-65 211
MsaG010532 AT2G25900.1 74.561 114 29 0 71 184 84 197 3.38e-65 211
MsaG010532 AT2G41900.1 67.241 116 38 0 73 188 257 372 3.89e-56 197
MsaG010532 AT5G58620.1 66.957 115 37 1 73 186 205 319 2.87e-54 190
MsaG010532 AT5G07500.1 68.182 110 35 0 70 179 45 154 1.83e-52 176
MsaG010532 AT5G12850.1 65.217 115 39 1 73 186 253 367 5.05e-52 185
MsaG010532 AT3G55980.1 38.976 254 102 5 28 270 156 367 3.22e-49 176
MsaG010532 AT2G40140.2 40.851 235 90 6 73 293 210 409 7.05e-49 175
MsaG010532 AT2G40140.1 40.851 235 90 6 73 293 210 409 7.05e-49 175
MsaG010532 AT3G55980.2 38.976 254 102 5 28 270 223 434 1.09e-48 175

Find 82 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCGGGTGAGGAGCTTTCT+TGG 0.183749 2:-74410731 None:intergenic
AGACATGCTTATTGATCTTT+TGG 0.193333 2:+74411468 MsaT010532.1:CDS
CATTGTGTGGGAGGAACTTT+TGG 0.221739 2:-74410799 None:intergenic
GAGGGATGTCGATGTCTCTA+AGG 0.318194 2:-74410709 None:intergenic
AAACAGCGACAATATTAATA+AGG 0.368587 2:+74411771 MsaT010532.1:CDS
CGGTGGTGTTGCGGCGGGTG+AGG 0.397490 2:-74410742 None:intergenic
TCATTCACCCATCCGAGATC+AGG 0.400183 2:-74411835 None:intergenic
GACAATATTAATAAGGTTGT+TGG 0.414119 2:+74411778 MsaT010532.1:CDS
TTTGGGAGAGAGAGGTTGGT+AGG 0.420760 2:-74410681 None:intergenic
CTCGTAGCCGGAGCCATGAC+TGG 0.421786 2:+74410897 MsaT010532.1:CDS
CGCCGCGGTGGCTGTAACCG+TGG 0.429136 2:+74411010 MsaT010532.1:CDS
GAAGATGATGATGATGTAAC+AGG 0.430284 2:-74411175 None:intergenic
CAGCCACCGCGGCGATACTC+AGG 0.436266 2:-74411001 None:intergenic
TCTTGTCCTGCCCCTGATCT+CGG 0.437151 2:+74411823 MsaT010532.1:CDS
GAAGATGTCGACGGCGGCGG+TGG 0.446476 2:-74410759 None:intergenic
AGTGCTTTAGCAGCAGAAAC+TGG 0.447660 2:-74411541 None:intergenic
AAGAATGAAACTTGTGTTGT+TGG 0.454570 2:-74410658 None:intergenic
TGTTGCTGATTCTGTTCCTC+AGG 0.458860 2:-74411604 None:intergenic
GGACTTTGAATTCATACATT+CGG 0.463131 2:-74410865 None:intergenic
CGGCGGCGGTGGTGTTGCGG+CGG 0.463780 2:-74410748 None:intergenic
GGCGGCGGTGGTGTTGCGGC+GGG 0.472525 2:-74410747 None:intergenic
GTCCTGCCCCTGATCTCGGA+TGG 0.476419 2:+74411827 MsaT010532.1:CDS
GGAGAAGATGTCGACGGCGG+CGG 0.481474 2:-74410762 None:intergenic
CGGGTGAGGAGCTTTCTTGG+AGG 0.487835 2:-74410728 None:intergenic
CGGAAATGATCTGATGAGTA+AGG 0.490767 2:-74410845 None:intergenic
GGGTGAGGAGCTTTCTTGGA+GGG 0.496924 2:-74410727 None:intergenic
TCCTGCCCCTGATCTCGGAT+GGG 0.499250 2:+74411828 MsaT010532.1:CDS
ATTAATAAGGTTGTTGGTGG+TGG 0.500154 2:+74411784 MsaT010532.1:CDS
GTTGGTGGTGGTGACGTCAA+TGG 0.503527 2:+74411796 MsaT010532.1:CDS
AGAACTGAAGCATGCAAAGA+TGG 0.503562 2:+74411091 MsaT010532.1:CDS
TCCGCCGGTGCACTCGTAGC+CGG 0.504444 2:+74410885 MsaT010532.1:CDS
ACACCGTTACGTGGCTTCAT+CGG 0.504782 2:-74411391 None:intergenic
GAACCATAGCGTGAGAGCGA+TGG 0.517489 2:-74411415 None:intergenic
CGACGGCGGCGGTGGTGTTG+CGG 0.519055 2:-74410751 None:intergenic
CGTGGCTTCATCGGAGACAT+AGG 0.522416 2:-74411382 None:intergenic
CCACGGCGGTTTCATTACTC+AGG 0.527158 2:+74410968 MsaT010532.1:CDS
CATTCACCCATCCGAGATCA+GGG 0.528306 2:-74411834 None:intergenic
TTGTCGACATCATTGAAACT+TGG 0.531596 2:-74411712 None:intergenic
GTTCCATCGCTCTCACGCTA+TGG 0.533114 2:+74411412 MsaT010532.1:CDS
CACTGTGGCGGTAATGGTAA+TGG 0.535187 2:+74411286 MsaT010532.1:CDS
ATCTGATGAGTAAGGATCAT+CGG 0.544740 2:-74410837 None:intergenic
AATATTAATAAGGTTGTTGG+TGG 0.545047 2:+74411781 MsaT010532.1:CDS
GCTTCAGTTCTGTATCTAGC+AGG 0.547143 2:-74411079 None:intergenic
GAAGGGATGAATTTCAATGA+CGG 0.555565 2:+74411499 MsaT010532.1:CDS
GGCGGTAATGGTAATGGTAA+TGG 0.562964 2:+74411292 MsaT010532.1:CDS
AATGAAACCGCCGTGGATCA+CGG 0.566037 2:-74410961 None:intergenic
AAGCTCGCCGCCGTGATCCA+CGG 0.567851 2:+74410951 MsaT010532.1:CDS
AGTCAGTCCAGTCATGGCTC+CGG 0.569804 2:-74410904 None:intergenic
TGATCAAAAGATGAAGAAGA+AGG 0.576719 2:-74411640 None:intergenic
GGAGACATAGGTGGTGATGC+CGG 0.581592 2:-74411370 None:intergenic
GATACTTGTGAATTTGCTCA+TGG 0.582670 2:+74411034 MsaT010532.1:CDS
ATGGACAGTCAGTCCAGTCA+TGG 0.586583 2:-74410910 None:intergenic
TGAATCTCTTGATGTTCCTG+AGG 0.587490 2:+74411588 MsaT010532.1:CDS
AGCCACCGCGGCGATACTCA+GGG 0.587575 2:-74411000 None:intergenic
ACCCATCCGAGATCAGGGGC+AGG 0.589319 2:-74411829 None:intergenic
GACTGTCCATTCGCTCACCC+CGG 0.597229 2:+74410923 MsaT010532.1:CDS
AGCACTTCAGATACAAAACA+TGG 0.598969 2:+74411558 MsaT010532.1:CDS
TCTCCGATGAAGCCACGTAA+CGG 0.601715 2:+74411388 MsaT010532.1:CDS
AGGGATGTCGATGTCTCTAA+GGG 0.602570 2:-74410708 None:intergenic
TCCGGCTACGAGTGCACCGG+CGG 0.607263 2:-74410886 None:intergenic
AGTCAGAGTCATTGTGTGGG+AGG 0.622332 2:-74410808 None:intergenic
TCTGAGTCAGAGTCATTGTG+TGG 0.623962 2:-74410812 None:intergenic
GGCTCCGGCTACGAGTGCAC+CGG 0.627207 2:-74410889 None:intergenic
ATGATGATGATGTAACAGGA+AGG 0.628815 2:-74411171 None:intergenic
TGCCCTGAGTATCGCCGCGG+TGG 0.630381 2:+74410998 MsaT010532.1:CDS
CACGGCGGTTTCATTACTCA+GGG 0.632004 2:+74410969 MsaT010532.1:CDS
CTGAGTCAGAGTCATTGTGT+GGG 0.635621 2:-74410811 None:intergenic
CAAATTCACAAGTATCGCCA+CGG 0.650826 2:-74411027 None:intergenic
AGCGATGGAACACCGTTACG+TGG 0.658393 2:-74411400 None:intergenic
GACATAGGTGGTGATGCCGG+AGG 0.666150 2:-74411367 None:intergenic
GTCGTCGGAGAAGATGTCGA+CGG 0.666964 2:-74410768 None:intergenic
GGAAGGATTCTTAATTGACG+AGG 0.669701 2:-74411154 None:intergenic
TGTATGAATTCAAAGTCCGC+CGG 0.674896 2:+74410870 MsaT010532.1:CDS
GTCGGAGAAGATGTCGACGG+CGG 0.678761 2:-74410765 None:intergenic
ATTCACCCATCCGAGATCAG+GGG 0.681023 2:-74411833 None:intergenic
GGCTTCATCGGAGACATAGG+TGG 0.681714 2:-74411379 None:intergenic
CCTGAGTAATGAAACCGCCG+TGG 0.683137 2:-74410968 None:intergenic
CTCGCCGCCGTGATCCACGG+CGG 0.698305 2:+74410954 MsaT010532.1:CDS
GCACTTCAGATACAAAACAT+GGG 0.708790 2:+74411559 MsaT010532.1:CDS
CGCCACGGTTACAGCCACCG+CGG 0.740750 2:-74411012 None:intergenic
GAAACCGCCGTGGATCACGG+CGG 0.763141 2:-74410958 None:intergenic
GTTTGCCCTGAGTATCGCCG+CGG 0.768550 2:+74410995 MsaT010532.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 74410632 74411870 74410632 ID=MsaG010532
Chr2 mRNA 74410632 74411870 74410632 ID=MsaT010532.1;Parent=MsaG010532
Chr2 exon 74410632 74411870 74410632 ID=MsaT010532.1.exon1;Parent=MsaT010532.1
Chr2 CDS 74410632 74411870 74410632 ID=cds.MsaT010532.1;Parent=MsaT010532.1
Gene Sequence

>MsaT010532.1

ATGACTACTATTTGTGCTGAACAACACCAACAACACAAGTTTCATTCTTCCTACCAACCTCTCTCTCCCAAAAAATCCCTTAGAGACATCGACATCCCTCCAAGAAAGCTCCTCACCCGCCGCAACACCACCGCCGCCGTCGACATCTTCTCCGACGACACCATCCTCCAAAAGTTCCTCCCACACAATGACTCTGACTCAGACTCCGATGATCCTTACTCATCAGATCATTTCCGAATGTATGAATTCAAAGTCCGCCGGTGCACTCGTAGCCGGAGCCATGACTGGACTGACTGTCCATTCGCTCACCCCGGTGAAAAAGCTCGCCGCCGTGATCCACGGCGGTTTCATTACTCAGGGACAGTTTGCCCTGAGTATCGCCGCGGTGGCTGTAACCGTGGCGATACTTGTGAATTTGCTCATGGTGTTTTTGAATGCTGGCTTCATCCTGCTAGATACAGAACTGAAGCATGCAAAGATGGTAAAAACTGTAAGAGAAAAGTTTGTTTCTTTGCACACACTCCTCGTCAATTAAGAATCCTTCCTGTTACATCATCATCATCTTCAAATGAACATTTTTCATGCAACAAGAAAAATAACAAGCTTTTGAATAACCATGTTGCTTCAAAATCAAACAATTGTTGTTTGTTTTGTCACTGTGGCGGTAATGGTAATGGTAATGGTGCTAATAATTCTCCAACTTCAACACTTTTTGGTATGTCTCATTTTTCTTCGCCTCCGGCATCACCACCTATGTCTCCGATGAAGCCACGTAACGGTGTTCCATCGCTCTCACGCTATGGTTCTTCGATTAATAGTAACAATCTTCGTTATAGAGACATGCTTATTGATCTTTTGGGTTCTTTTGAAGGGATGAATTTCAATGACGGTTCATCTTCTTCTTCTTTACCAGTTTCTGCTGCTAAAGCACTTCAGATACAAAACATGGGTTATCTTGAATCTCTTGATGTTCCTGAGGAACAGAATCAGCAACAGTTTATTGTTTCACCTTCTTCTTCATCTTTTGATCATCAACAACATTTCAATATTCTTTCACAATCTATTCATCAGAATTTGACACCAAGTTTCAATGATGTCGACAATAACATCATTAACAACAATATGAACATGTTTTTGGGAAACAGCGACAATATTAATAAGGTTGTTGGTGGTGGTGACGTCAATGGTTTGTCTTGTCCTGCCCCTGATCTCGGATGGGTGAATGAATTGCTGATGTAG
Protein sequence

>MsaT010532.1

MTTICAEQHQQHKFHSSYQPLSPKKSLRDIDIPPRKLLTRRNTTAAVDIFSDDTILQKFLPHNDSDSDSDDPYSSDHFRMYEFKVRRCTRSRSHDWTDCPFAHPGEKARRRDPRRFHYSGTVCPEYRRGGCNRGDTCEFAHGVFECWLHPARYRTEACKDGKNCKRKVCFFAHTPRQLRILPVTSSSSSNEHFSCNKKNNKLLNNHVASKSNNCCLFCHCGGNGNGNGANNSPTSTLFGMSHFSSPPASPPMSPMKPRNGVPSLSRYGSSINSNNLRYRDMLIDLLGSFEGMNFNDGSSSSSLPVSAAKALQIQNMGYLESLDVPEEQNQQQFIVSPSSSSFDHQQHFNILSQSIHQNLTPSFNDVDNNIINNNMNMFLGNSDNINKVVGGGDVNGLSCPAPDLGWVNELLM*