AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010545


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010545 MtrunA17_Chr2g0324511 81.595 163 20 2 1 153 1 163 1.64e-88 258
MsaG010545 MtrunA17_Chr4g0040201 66.495 194 57 4 9 194 8 201 1.60e-83 246
MsaG010545 MtrunA17_Chr2g0324521 86.957 115 9 2 45 153 2 116 3.03e-63 192
MsaG010545 MtrunA17_Chr2g0324571 80.342 117 15 1 45 153 2 118 4.19e-62 189
MsaG010545 MtrunA17_Chr2g0324531 86.441 59 8 0 152 210 2 60 2.11e-31 109
MsaG010545 MtrunA17_Chr2g0282341 39.552 134 69 3 48 173 4 133 4.18e-28 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010545 AT4G20970.1 40.884 181 87 6 12 175 11 188 1.17e-33 119
MsaG010545 AT1G10585.1 28.902 173 117 3 7 177 9 177 1.82e-14 68.9
MsaG010545 AT5G51780.1 29.586 169 105 3 14 169 3 170 3.99e-12 62.8
MsaG010545 AT5G51780.2 29.586 169 105 3 14 169 26 193 6.11e-12 62.4
MsaG010545 AT1G10586.1 25.882 170 120 3 7 174 9 174 4.88e-11 59.7

Find 41 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACAAACTTTGTTACAATT+TGG 0.173105 2:+74569726 MsaT010545.1:CDS
TATCTCTTCAAATTCTTCTT+TGG 0.234992 2:+74568785 MsaT010545.1:CDS
CAATTGTGGGATCTTGAAAT+TGG 0.247122 2:+74569819 MsaT010545.1:CDS
ATGCTTCTTAATTAAGGATT+TGG 0.262973 2:-74569888 None:intergenic
AACAAGAAAGAAAGTTTAAA+AGG 0.273993 2:+74568630 MsaT010545.1:CDS
AGAGAAGAAAGAAAGTTTAA+TGG 0.275748 2:+74568605 MsaT010545.1:CDS
GAGAGTTAAGTTTGGAATAG+AGG 0.305206 2:-74567870 None:intergenic
GAGAAGAAAGAAAGTTTAAT+GGG 0.355968 2:+74568606 MsaT010545.1:CDS
CCAACTTTAGATTTGTCTCT+AGG 0.356186 2:-74568578 None:intergenic
AGGAAGAAGAGAGTTAAGTT+TGG 0.360183 2:-74567878 None:intergenic
CCAAATTGTAACAAAGTTTG+TGG 0.389197 2:-74569726 None:intergenic
AACATCCTTTCTTTCGACTT+TGG 0.397896 2:-74567818 None:intergenic
TCTTCAAATTCTTCTTTGGC+TGG 0.403407 2:+74568789 MsaT010545.1:CDS
TTGAAATTGGAAGTGAGAGT+TGG 0.404544 2:+74569832 MsaT010545.1:CDS
TAATTTCACAGAAAATAAAA+TGG 0.425918 2:-74568731 None:intergenic
ATAGCCTCATCTACTTGATC+AGG 0.429867 2:-74568540 None:intergenic
CTTTCGACTTTGGTTGAACA+AGG 0.449950 2:-74567808 None:intergenic
AAAATAGAGATTCATGAAAT+GGG 0.456591 2:+74568669 MsaT010545.1:CDS
TGAAATTGGAAGTGAGAGTT+GGG 0.469657 2:+74569833 MsaT010545.1:CDS
TCTTCATACCGTGCATGCTC+AGG 0.472764 2:+74568821 MsaT010545.1:CDS
TCTTGTTGATTATGTTCCAT+TGG 0.483862 2:-74567778 None:intergenic
TTCAACCAAAGTCGAAAGAA+AGG 0.492351 2:+74567813 MsaT010545.1:CDS
CCTAGAGACAAATCTAAAGT+TGG 0.498284 2:+74568578 MsaT010545.1:CDS
ATTTCAAGATCCCACAATTG+AGG 0.509351 2:-74569816 None:intergenic
TTAAGGATTTGGTAAACTCA+TGG 0.514490 2:-74569877 None:intergenic
TCATCTACTTGATCAGGTAA+TGG 0.522136 2:-74568534 None:intergenic
TACAAGTGATTGTAACATGT+GGG 0.544345 2:+74568700 MsaT010545.1:CDS
GGAAACACAGCCTCAATTGT+GGG 0.559018 2:+74569806 MsaT010545.1:CDS
TGGAAACACAGCCTCAATTG+TGG 0.568137 2:+74569805 MsaT010545.1:CDS
ATGACCTTCGAATTATAGCT+AGG 0.581881 2:-74567898 None:intergenic
TCTTCCTAGCTATAATTCGA+AGG 0.587922 2:+74567894 MsaT010545.1:CDS
TATATATACCTGAGCATGCA+CGG 0.590008 2:-74568829 None:intergenic
GAGCAATCAAAATAAGTGAG+AGG 0.593941 2:+74569748 MsaT010545.1:CDS
TGTTACAATCACTTGTAGAG+TGG 0.604182 2:-74568694 None:intergenic
GTTACAATCACTTGTAGAGT+GGG 0.605139 2:-74568693 None:intergenic
CTACAAGTGATTGTAACATG+TGG 0.615945 2:+74568699 MsaT010545.1:CDS
AGGTTGAAAGAGTTTGTGAA+CGG 0.618184 2:+74569768 MsaT010545.1:CDS
ATTACCTGATCAAGTAGATG+AGG 0.636526 2:+74568536 MsaT010545.1:CDS
GAACGGATCTGTGAGCGATG+TGG 0.701045 2:+74569785 MsaT010545.1:CDS
ACAAGTGATTGTAACATGTG+GGG 0.739715 2:+74568701 MsaT010545.1:CDS
ACTGCATGAAGAGAACGTCG+AGG 0.768331 2:+74568761 MsaT010545.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 74567781 74569899 74567781 ID=MsaG010545
Chr2 mRNA 74567781 74569899 74567781 ID=MsaT010545.1;Parent=MsaG010545
Chr2 exon 74567781 74567915 74567781 ID=MsaT010545.1.exon1;Parent=MsaT010545.1
Chr2 CDS 74567781 74567915 74567781 ID=cds.MsaT010545.1;Parent=MsaT010545.1
Chr2 exon 74568522 74568842 74568522 ID=MsaT010545.1.exon2;Parent=MsaT010545.1
Chr2 CDS 74568522 74568842 74568522 ID=cds.MsaT010545.1;Parent=MsaT010545.1
Chr2 exon 74569723 74569899 74569723 ID=MsaT010545.1.exon3;Parent=MsaT010545.1
Chr2 CDS 74569723 74569899 74569723 ID=cds.MsaT010545.1;Parent=MsaT010545.1
Gene Sequence

>MsaT010545.1

ATGGAACATAATCAACAAGAATGTCAACCTTGTTCAACCAAAGTCGAAAGAAAGGATGTTGAGAAAAATAGGAGAAATCAGATGAAGATCCTCTATTCCAAACTTAACTCTCTTCTTCCTAGCTATAATTCGAAGGAATTAACGTTACCATTACCTGATCAAGTAGATGAGGCTATAAACTACATAAAGAGCCTAGAGACAAATCTAAAGTTGGCAAAAGAGAAGAAAGAAAGTTTAATGGGAAACAAGAAAGAAAGTTTAAAAGGAAGCATAAAGTTGCCAAAAATAGAGATTCATGAAATGGGACCCACTCTACAAGTGATTGTAACATGTGGGGTTGATGACCATTTTATTTTCTGTGAAATTATGAGAATACTGCATGAAGAGAACGTCGAGGTTATCTCTTCAAATTCTTCTTTGGCTGGTGATTCACTTCTTCATACCGTGCATGCTCAGATTCCACAAACTTTGTTACAATTTGGAGCAATCAAAATAAGTGAGAGGTTGAAAGAGTTTGTGAACGGATCTGTGAGCGATGTGGAAACACAGCCTCAATTGTGGGATCTTGAAATTGGAAGTGAGAGTTGGGAGCTTCTACATTCTGTAGTAACCATGAGTTTACCAAATCCTTAA

Protein sequence

>MsaT010545.1

MEHNQQECQPCSTKVERKDVEKNRRNQMKILYSKLNSLLPSYNSKELTLPLPDQVDEAINYIKSLETNLKLAKEKKESLMGNKKESLKGSIKLPKIEIHEMGPTLQVIVTCGVDDHFIFCEIMRILHEENVEVISSNSSLAGDSLLHTVHAQIPQTLLQFGAIKISERLKEFVNGSVSDVETQPQLWDLEIGSESWELLHSVVTMSLPNP*