Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG010545 | XP_013465109.1 | 85.714 | 217 | 24 | 3 | 1 | 210 | 1 | 217 | 2.55e-125 | 363 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG010545 | sp|F4JIJ7|BH162_ARATH | 40.884 | 181 | 87 | 6 | 12 | 175 | 11 | 188 | 1.15e-32 | 119 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG010545 | tr|A0A072VAT4|A0A072VAT4_MEDTR | 85.714 | 217 | 24 | 3 | 1 | 210 | 1 | 217 | 1.22e-125 | 363 |
Gene ID | Type | Classification |
---|---|---|
MsaG010545 | TF | bHLH |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000459 | MsaG010545 | 0.803346 | 6.131363e-49 | 8.945954e-47 |
MsaG001273 | MsaG010545 | 0.846216 | 4.696256e-59 | 2.240236e-56 |
MsaG002043 | MsaG010545 | 0.806416 | 1.409313e-49 | 2.208829e-47 |
MsaG002718 | MsaG010545 | 0.800494 | 2.347440e-48 | 3.209462e-46 |
MsaG003503 | MsaG010545 | 0.820162 | 1.389985e-52 | 3.070336e-50 |
MsaG003632 | MsaG010545 | 0.809377 | 3.328985e-50 | 5.599906e-48 |
MsaG003715 | MsaG010545 | 0.811398 | 1.225147e-50 | 2.164875e-48 |
MsaG003795 | MsaG010545 | 0.836054 | 2.140523e-56 | 7.399898e-54 |
MsaG003894 | MsaG010545 | 0.806683 | 1.238748e-49 | 1.953706e-47 |
MsaG003966 | MsaG010545 | 0.810156 | 2.267439e-50 | 3.886820e-48 |
MsaG004280 | MsaG010545 | 0.801115 | 1.755562e-48 | 2.434116e-46 |
MsaG004556 | MsaG010545 | 0.811604 | 1.105192e-50 | 1.962824e-48 |
MsaG004681 | MsaG010545 | 0.814430 | 2.671864e-51 | 5.091438e-49 |
MsaG005005 | MsaG010545 | 0.840636 | 1.428130e-57 | 5.687419e-55 |
MsaG005641 | MsaG010545 | 0.827509 | 2.685711e-54 | 7.243784e-52 |
MsaG006122 | MsaG010545 | 0.802743 | 8.159667e-49 | 1.174066e-46 |
MsaG006335 | MsaG010545 | 0.811418 | 1.212749e-50 | 2.144032e-48 |
MsaG006351 | MsaG010545 | 0.834294 | 5.923709e-56 | 1.942768e-53 |
MsaG006422 | MsaG010545 | 0.828680 | 1.406921e-54 | 3.921464e-52 |
MsaG006610 | MsaG010545 | 0.853265 | 5.148552e-61 | 3.126735e-58 |
MsaG006623 | MsaG010545 | 0.804922 | 2.891613e-49 | 4.375922e-47 |
MsaG006655 | MsaG010545 | 0.804475 | 3.581240e-49 | 5.363558e-47 |
MsaG006638 | MsaG010545 | 0.811037 | 1.465398e-50 | 2.566633e-48 |
MsaG006751 | MsaG010545 | 0.824361 | 1.489939e-53 | 3.683263e-51 |
MsaG007182 | MsaG010545 | 0.834820 | 4.374885e-56 | 1.457575e-53 |
MsaG007551 | MsaG010545 | 0.803633 | 5.349187e-49 | 7.856553e-47 |
MsaG008238 | MsaG010545 | 0.825556 | 7.808466e-54 | 1.994507e-51 |
MsaG009121 | MsaG010545 | 0.800931 | 1.913719e-48 | 2.642381e-46 |
MsaG010052 | MsaG010545 | 0.807970 | 6.628162e-50 | 1.077864e-47 |
MsaG010545 | MsaG012489 | 0.848901 | 8.650307e-60 | 4.515839e-57 |
MsaG010545 | MsaG013333 | 0.800685 | 2.147684e-48 | 2.949149e-46 |
MsaG010545 | MsaG016663 | 0.803277 | 6.335000e-49 | 9.228417e-47 |
MsaG010545 | MsaG017057 | 0.811602 | 1.106600e-50 | 1.965213e-48 |
MsaG010545 | MsaG017310 | 0.816143 | 1.116528e-51 | 2.221896e-49 |
MsaG010545 | MsaG017317 | 0.809638 | 2.926509e-50 | 4.954179e-48 |
MsaG010545 | MsaG017719 | 0.800840 | 1.997299e-48 | 2.752142e-46 |
MsaG010545 | MsaG017828 | 0.813440 | 4.407498e-51 | 8.192674e-49 |
MsaG010545 | MsaG018078 | 0.828956 | 1.207311e-54 | 3.391206e-52 |
MsaG010545 | MsaG018214 | 0.816960 | 7.342732e-52 | 1.492082e-49 |
MsaG010545 | MsaG018577 | 0.845986 | 5.420313e-59 | 2.565960e-56 |
MsaG010545 | MsaG018793 | 0.801404 | 1.533379e-48 | 2.140001e-46 |
MsaG010545 | MsaG019395 | 0.809630 | 2.939424e-50 | 4.974979e-48 |
MsaG010545 | MsaG019525 | 0.816590 | 8.877168e-52 | 1.786791e-49 |
MsaG010545 | MsaG019910 | 0.835110 | 3.700375e-56 | 1.243576e-53 |
MsaG010545 | MsaG020031 | 0.811557 | 1.131523e-50 | 2.007273e-48 |
MsaG010545 | MsaG020346 | 0.831205 | 3.434481e-55 | 1.028849e-52 |
MsaG010545 | MsaG021294 | 0.803309 | 6.240587e-49 | 9.097361e-47 |
MsaG010545 | MsaG023376 | 0.803422 | 5.912691e-49 | 8.641965e-47 |
MsaG010545 | MsaG024008 | 0.817549 | 5.420058e-52 | 1.118208e-49 |
MsaG010545 | MsaG024303 | 0.831390 | 3.094298e-55 | 9.319829e-53 |
MsaG010545 | MsaG024453 | 0.810331 | 2.079569e-50 | 3.580148e-48 |
MsaG010545 | MsaG024480 | 0.817231 | 6.384504e-52 | 1.306516e-49 |
MsaG010545 | MsaG026022 | 0.801963 | 1.178689e-48 | 1.666040e-46 |
MsaG010545 | MsaG026607 | 0.808474 | 5.182093e-50 | 8.529966e-48 |
MsaG010545 | MsaG026786 | 0.851387 | 1.752849e-60 | 9.965428e-58 |
MsaG010545 | MsaG026963 | 0.807453 | 8.526845e-50 | 1.369498e-47 |
MsaG010545 | MsaG027307 | 0.818533 | 3.254923e-52 | 6.888011e-50 |
MsaG010545 | MsaG027822 | 0.806040 | 1.689857e-49 | 2.624880e-47 |
MsaG010545 | MsaG027763 | 0.825503 | 8.035314e-54 | 2.049450e-51 |
MsaG010545 | MsaG027877 | 0.804596 | 3.379429e-49 | 5.075510e-47 |
MsaG010545 | MsaG028135 | 0.821302 | 7.626765e-53 | 1.736199e-50 |
MsaG010545 | MsaG028248 | 0.816200 | 1.084768e-51 | 2.161727e-49 |
MsaG010545 | MsaG029091 | 0.801167 | 1.713481e-48 | 2.378556e-46 |
MsaG010545 | MsaG029422 | 0.816112 | 1.134372e-51 | 2.255524e-49 |
MsaG010545 | MsaG029752 | 0.816137 | 1.120106e-51 | 2.228613e-49 |
MsaG010545 | MsaG030902 | 0.804204 | 4.076570e-49 | 6.067346e-47 |
MsaG010545 | MsaG032279 | 0.803580 | 5.484971e-49 | 8.046227e-47 |
MsaG010545 | MsaG032702 | 0.842530 | 4.550190e-58 | 1.924716e-55 |
MsaG010545 | MsaG033995 | 0.862036 | 1.331390e-63 | 1.118970e-60 |
MsaG010545 | MsaG034590 | 0.833306 | 1.043256e-55 | 3.322993e-53 |
MsaG010545 | MsaG035037 | 0.853978 | 3.219377e-61 | 2.005456e-58 |
MsaG010545 | MsaG038749 | 0.822624 | 3.780300e-53 | 8.915193e-51 |
MsaG010545 | MsaG039468 | 0.820285 | 1.303301e-52 | 2.888236e-50 |
MsaG010545 | MsaG040095 | 0.855575 | 1.114327e-61 | 7.350580e-59 |
MsaG010545 | MsaG040379 | 0.807112 | 1.006299e-49 | 1.603162e-47 |
MsaG010545 | MsaG041202 | 0.831396 | 3.084274e-55 | 9.291421e-53 |
MsaG010545 | MsaG043237 | 0.806857 | 1.138619e-49 | 1.803093e-47 |
MsaG010545 | MsaG043817 | 0.802416 | 9.522702e-49 | 1.360039e-46 |
MsaG010545 | MsaG044019 | 0.806054 | 1.678446e-49 | 2.608057e-47 |
MsaG010545 | MsaG044303 | 0.815866 | 1.286566e-51 | 2.542168e-49 |
MsaG010545 | MsaG044546 | 0.806739 | 1.205694e-49 | 1.904070e-47 |
MsaG010545 | MsaG044617 | 0.803453 | 5.827222e-49 | 8.523117e-47 |
MsaG010545 | MsaG045439 | 0.821929 | 5.470940e-53 | 1.266322e-50 |
MsaG010545 | MsaG045535 | 0.809699 | 2.841236e-50 | 4.816795e-48 |
MsaG010545 | MsaG045778 | 0.837138 | 1.136412e-56 | 4.059417e-54 |
MsaG010545 | MsaG046104 | 0.808806 | 4.405916e-50 | 7.310463e-48 |
MsaG010545 | MsaG046180 | 0.833861 | 7.595431e-56 | 2.458968e-53 |
MsaG010545 | MsaG046540 | 0.806493 | 1.357585e-49 | 2.131590e-47 |
MsaG010545 | MsaG046780 | 0.814858 | 2.150106e-51 | 4.141546e-49 |
MsaG010545 | MsaG047011 | 0.822141 | 4.888270e-53 | 1.137924e-50 |
MsaG010545 | MsaG001950 | 0.839700 | 2.500547e-57 | 9.670116e-55 |
MsaG010545 | MsaG001966 | 0.809861 | 2.622904e-50 | 4.464128e-48 |
MsaG010545 | MsaG002578 | 0.806283 | 1.503114e-49 | 2.348380e-47 |
MsaG010545 | MsaG002617 | 0.801970 | 1.175268e-48 | 1.661439e-46 |
MsaG010545 | MsaG014278 | 0.802063 | 1.124643e-48 | 1.593259e-46 |
MsaG010545 | MsaG017584 | 0.837962 | 7.002800e-57 | 2.565384e-54 |
MsaG010545 | MsaG012272 | 0.825567 | 7.759868e-54 | 1.982713e-51 |
MsaG010545 | MsaG019880 | 0.821933 | 5.459824e-53 | 1.263869e-50 |
MsaG010545 | MsaG018939 | 0.809585 | 3.004605e-50 | 5.080044e-48 |
MsaG010545 | MsaG026803 | 0.803698 | 5.186000e-49 | 7.628557e-47 |
MsaG010545 | MsaG033077 | 0.833720 | 8.232210e-56 | 2.654320e-53 |
MsaG010545 | MsaG031964 | 0.807551 | 8.127239e-50 | 1.308416e-47 |
MsaG010545 | MsaG034262 | 0.808289 | 5.671515e-50 | 9.294085e-48 |
MsaG010545 | MsaG031242 | 0.824552 | 1.344605e-53 | 3.341204e-51 |
MsaG010545 | MsaG030550 | 0.805074 | 2.688448e-49 | 4.082844e-47 |
MsaG010545 | MsaG033449 | 0.825858 | 6.625004e-54 | 1.706569e-51 |
MsaG010545 | MsaG033259 | 0.810203 | 2.215341e-50 | 3.801841e-48 |
MsaG010545 | MsaG034891 | 0.839673 | 2.540864e-57 | 9.817469e-55 |
MsaG010545 | MsaG036736 | 0.801786 | 1.281602e-48 | 1.804176e-46 |
MsaG010545 | MsaG037155 | 0.823660 | 2.172404e-53 | 5.269090e-51 |
MsaG010545 | MsaG035248 | 0.814337 | 2.801121e-51 | 5.325185e-49 |
MsaG010545 | MsaG037051 | 0.800701 | 2.131074e-48 | 2.927413e-46 |
MsaG010545 | MsaG039945 | 0.861854 | 1.513549e-63 | 1.262970e-60 |
MsaG010545 | MsaG035299 | 0.812018 | 8.990232e-51 | 1.612985e-48 |
MsaG010545 | MsaG046905 | 0.814661 | 2.376250e-51 | 4.554346e-49 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG010545 | MtrunA17_Chr2g0324511 | 81.595 | 163 | 20 | 2 | 1 | 153 | 1 | 163 | 1.64e-88 | 258 |
MsaG010545 | MtrunA17_Chr4g0040201 | 66.495 | 194 | 57 | 4 | 9 | 194 | 8 | 201 | 1.60e-83 | 246 |
MsaG010545 | MtrunA17_Chr2g0324521 | 86.957 | 115 | 9 | 2 | 45 | 153 | 2 | 116 | 3.03e-63 | 192 |
MsaG010545 | MtrunA17_Chr2g0324571 | 80.342 | 117 | 15 | 1 | 45 | 153 | 2 | 118 | 4.19e-62 | 189 |
MsaG010545 | MtrunA17_Chr2g0324531 | 86.441 | 59 | 8 | 0 | 152 | 210 | 2 | 60 | 2.11e-31 | 109 |
MsaG010545 | MtrunA17_Chr2g0282341 | 39.552 | 134 | 69 | 3 | 48 | 173 | 4 | 133 | 4.18e-28 | 103 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG010545 | AT4G20970.1 | 40.884 | 181 | 87 | 6 | 12 | 175 | 11 | 188 | 1.17e-33 | 119 |
MsaG010545 | AT1G10585.1 | 28.902 | 173 | 117 | 3 | 7 | 177 | 9 | 177 | 1.82e-14 | 68.9 |
MsaG010545 | AT5G51780.1 | 29.586 | 169 | 105 | 3 | 14 | 169 | 3 | 170 | 3.99e-12 | 62.8 |
MsaG010545 | AT5G51780.2 | 29.586 | 169 | 105 | 3 | 14 | 169 | 26 | 193 | 6.11e-12 | 62.4 |
MsaG010545 | AT1G10586.1 | 25.882 | 170 | 120 | 3 | 7 | 174 | 9 | 174 | 4.88e-11 | 59.7 |
Find 41 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
CCACAAACTTTGTTACAATT+TGG | 0.173105 | 2:+74569726 | MsaT010545.1:CDS |
TATCTCTTCAAATTCTTCTT+TGG | 0.234992 | 2:+74568785 | MsaT010545.1:CDS |
CAATTGTGGGATCTTGAAAT+TGG | 0.247122 | 2:+74569819 | MsaT010545.1:CDS |
ATGCTTCTTAATTAAGGATT+TGG | 0.262973 | 2:-74569888 | None:intergenic |
AACAAGAAAGAAAGTTTAAA+AGG | 0.273993 | 2:+74568630 | MsaT010545.1:CDS |
AGAGAAGAAAGAAAGTTTAA+TGG | 0.275748 | 2:+74568605 | MsaT010545.1:CDS |
GAGAGTTAAGTTTGGAATAG+AGG | 0.305206 | 2:-74567870 | None:intergenic |
GAGAAGAAAGAAAGTTTAAT+GGG | 0.355968 | 2:+74568606 | MsaT010545.1:CDS |
CCAACTTTAGATTTGTCTCT+AGG | 0.356186 | 2:-74568578 | None:intergenic |
AGGAAGAAGAGAGTTAAGTT+TGG | 0.360183 | 2:-74567878 | None:intergenic |
CCAAATTGTAACAAAGTTTG+TGG | 0.389197 | 2:-74569726 | None:intergenic |
AACATCCTTTCTTTCGACTT+TGG | 0.397896 | 2:-74567818 | None:intergenic |
TCTTCAAATTCTTCTTTGGC+TGG | 0.403407 | 2:+74568789 | MsaT010545.1:CDS |
TTGAAATTGGAAGTGAGAGT+TGG | 0.404544 | 2:+74569832 | MsaT010545.1:CDS |
TAATTTCACAGAAAATAAAA+TGG | 0.425918 | 2:-74568731 | None:intergenic |
ATAGCCTCATCTACTTGATC+AGG | 0.429867 | 2:-74568540 | None:intergenic |
CTTTCGACTTTGGTTGAACA+AGG | 0.449950 | 2:-74567808 | None:intergenic |
AAAATAGAGATTCATGAAAT+GGG | 0.456591 | 2:+74568669 | MsaT010545.1:CDS |
TGAAATTGGAAGTGAGAGTT+GGG | 0.469657 | 2:+74569833 | MsaT010545.1:CDS |
TCTTCATACCGTGCATGCTC+AGG | 0.472764 | 2:+74568821 | MsaT010545.1:CDS |
TCTTGTTGATTATGTTCCAT+TGG | 0.483862 | 2:-74567778 | None:intergenic |
TTCAACCAAAGTCGAAAGAA+AGG | 0.492351 | 2:+74567813 | MsaT010545.1:CDS |
CCTAGAGACAAATCTAAAGT+TGG | 0.498284 | 2:+74568578 | MsaT010545.1:CDS |
ATTTCAAGATCCCACAATTG+AGG | 0.509351 | 2:-74569816 | None:intergenic |
TTAAGGATTTGGTAAACTCA+TGG | 0.514490 | 2:-74569877 | None:intergenic |
TCATCTACTTGATCAGGTAA+TGG | 0.522136 | 2:-74568534 | None:intergenic |
TACAAGTGATTGTAACATGT+GGG | 0.544345 | 2:+74568700 | MsaT010545.1:CDS |
GGAAACACAGCCTCAATTGT+GGG | 0.559018 | 2:+74569806 | MsaT010545.1:CDS |
TGGAAACACAGCCTCAATTG+TGG | 0.568137 | 2:+74569805 | MsaT010545.1:CDS |
ATGACCTTCGAATTATAGCT+AGG | 0.581881 | 2:-74567898 | None:intergenic |
TCTTCCTAGCTATAATTCGA+AGG | 0.587922 | 2:+74567894 | MsaT010545.1:CDS |
TATATATACCTGAGCATGCA+CGG | 0.590008 | 2:-74568829 | None:intergenic |
GAGCAATCAAAATAAGTGAG+AGG | 0.593941 | 2:+74569748 | MsaT010545.1:CDS |
TGTTACAATCACTTGTAGAG+TGG | 0.604182 | 2:-74568694 | None:intergenic |
GTTACAATCACTTGTAGAGT+GGG | 0.605139 | 2:-74568693 | None:intergenic |
CTACAAGTGATTGTAACATG+TGG | 0.615945 | 2:+74568699 | MsaT010545.1:CDS |
AGGTTGAAAGAGTTTGTGAA+CGG | 0.618184 | 2:+74569768 | MsaT010545.1:CDS |
ATTACCTGATCAAGTAGATG+AGG | 0.636526 | 2:+74568536 | MsaT010545.1:CDS |
GAACGGATCTGTGAGCGATG+TGG | 0.701045 | 2:+74569785 | MsaT010545.1:CDS |
ACAAGTGATTGTAACATGTG+GGG | 0.739715 | 2:+74568701 | MsaT010545.1:CDS |
ACTGCATGAAGAGAACGTCG+AGG | 0.768331 | 2:+74568761 | MsaT010545.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr2 | gene | 74567781 | 74569899 | 74567781 | ID=MsaG010545 |
Chr2 | mRNA | 74567781 | 74569899 | 74567781 | ID=MsaT010545.1;Parent=MsaG010545 |
Chr2 | exon | 74567781 | 74567915 | 74567781 | ID=MsaT010545.1.exon1;Parent=MsaT010545.1 |
Chr2 | CDS | 74567781 | 74567915 | 74567781 | ID=cds.MsaT010545.1;Parent=MsaT010545.1 |
Chr2 | exon | 74568522 | 74568842 | 74568522 | ID=MsaT010545.1.exon2;Parent=MsaT010545.1 |
Chr2 | CDS | 74568522 | 74568842 | 74568522 | ID=cds.MsaT010545.1;Parent=MsaT010545.1 |
Chr2 | exon | 74569723 | 74569899 | 74569723 | ID=MsaT010545.1.exon3;Parent=MsaT010545.1 |
Chr2 | CDS | 74569723 | 74569899 | 74569723 | ID=cds.MsaT010545.1;Parent=MsaT010545.1 |
Gene Sequence |
Protein sequence |