AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010571

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010571 MtrunA17_Chr2g0326001 91.667 120 10 0 66 185 133 252 1.36e-69 213
MsaG010571 MtrunA17_Chr2g0326001 93.421 76 5 0 1 76 1 76 8.11e-38 132
MsaG010571 MtrunA17_Chr4g0030051 75.000 56 14 0 17 72 18 73 8.28e-25 97.8
MsaG010571 MtrunA17_Chr1g0188851 60.274 73 25 2 8 76 7 79 2.35e-23 94.0
MsaG010571 MtrunA17_Chr3g0127201 86.364 44 6 0 29 72 16 59 1.84e-22 91.7
MsaG010571 MtrunA17_Chr8g0346551 66.667 54 16 1 23 76 9 60 1.07e-19 84.0
MsaG010571 MtrunA17_Chr3g0117601 56.522 69 27 2 11 76 29 97 2.62e-19 84.0
MsaG010571 MtrunA17_Chr4g0010151 52.857 70 20 1 7 76 62 118 6.34e-19 83.2
MsaG010571 MtrunA17_Chr4g0031411 66.667 51 17 0 22 72 57 107 3.15e-18 80.9
MsaG010571 MtrunA17_Chr2g0328021 55.385 65 28 1 8 72 31 94 1.13e-17 78.6
MsaG010571 MtrunA17_Chr3g0126201 70.000 50 15 0 23 72 34 83 1.52e-17 79.0
MsaG010571 MtrunA17_Chr5g0411951 81.081 37 7 0 36 72 39 75 1.88e-16 75.9
MsaG010571 MtrunA17_Chr8g0374111 66.000 50 16 1 24 72 12 61 6.18e-16 73.2
MsaG010571 MtrunA17_Chr2g0282441 69.767 43 10 1 34 76 22 61 2.67e-15 72.8
MsaG010571 MtrunA17_Chr8g0367161 68.182 44 14 0 29 72 21 64 3.67e-15 72.4
MsaG010571 MtrunA17_Chr3g0092111 42.453 106 52 5 26 127 123 223 4.32e-15 72.4
MsaG010571 MtrunA17_Chr8g0374251 77.778 36 8 0 37 72 65 100 4.42e-15 72.0
MsaG010571 MtrunA17_Chr5g0417571 64.444 45 15 1 28 72 127 170 4.69e-15 72.0
MsaG010571 MtrunA17_Chr2g0308011 73.684 38 10 0 39 76 36 73 9.58e-15 69.7
MsaG010571 MtrunA17_Chr1g0177251 90.625 32 3 0 41 72 13 44 9.90e-15 70.5
MsaG010571 MtrunA17_Chr2g0282511 71.053 38 11 0 39 76 44 81 1.68e-14 70.1
MsaG010571 MtrunA17_Chr7g0237921 54.545 55 25 0 22 76 58 112 3.31e-14 69.7
MsaG010571 MtrunA17_Chr2g0282051 71.429 35 10 0 38 72 44 78 7.11e-14 68.2
MsaG010571 MtrunA17_Chr8g0341671 62.222 45 17 0 28 72 8 52 1.23e-13 66.6
MsaG010571 MtrunA17_Chr7g0223311 79.412 34 7 0 39 72 76 109 1.73e-13 67.8
MsaG010571 MtrunA17_Chr4g0061761 71.429 35 10 0 38 72 38 72 1.74e-13 67.4
MsaG010571 MtrunA17_Chr6g0460721 46.552 58 30 1 15 72 65 121 2.22e-13 67.4
MsaG010571 MtrunA17_Chr4g0047001 80.645 31 6 0 42 72 57 87 2.41e-13 67.4
MsaG010571 MtrunA17_Chr8g0355261 59.574 47 18 1 26 72 105 150 4.83e-13 66.6
MsaG010571 MtrunA17_Chr4g0047761 71.429 35 10 0 38 72 70 104 6.53e-13 65.9
MsaG010571 MtrunA17_Chr7g0217581 50.000 60 22 1 21 72 205 264 7.58e-13 66.2
MsaG010571 MtrunA17_Chr6g0460731 46.552 58 30 1 15 72 72 128 9.52e-13 65.5
MsaG010571 MtrunA17_Chr4g0042481 56.818 44 19 0 29 72 111 154 1.65e-12 65.1
MsaG010571 MtrunA17_Chr7g0254161 60.526 38 15 0 35 72 96 133 2.38e-12 64.7
MsaG010571 MtrunA17_Chr6g0453321 63.158 38 14 0 35 72 131 168 2.90e-12 64.3
MsaG010571 MtrunA17_Chr2g0283811 63.889 36 13 0 37 72 44 79 1.15e-11 60.5
MsaG010571 MtrunA17_Chr5g0417601 44.286 70 33 2 8 72 104 172 1.33e-11 62.4
MsaG010571 MtrunA17_Chr5g0417551 45.455 66 33 2 8 72 96 159 2.01e-11 61.6
MsaG010571 MtrunA17_Chr5g0417561 64.865 37 13 0 36 72 115 151 2.61e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010571 AT4G24060.1 77.083 48 11 0 29 76 42 89 1.70e-21 90.1
MsaG010571 AT1G64620.1 84.091 44 7 0 29 72 38 81 1.03e-20 88.2
MsaG010571 AT5G60200.1 64.407 59 13 1 26 76 33 91 6.09e-20 84.7
MsaG010571 AT2G46590.2 84.091 44 5 1 29 72 71 112 1.08e-19 85.5
MsaG010571 AT2G46590.1 84.091 44 5 1 29 72 59 100 1.10e-19 85.5
MsaG010571 AT3G45610.1 75.000 48 11 1 25 72 26 72 1.99e-19 83.2
MsaG010571 AT3G55370.1 64.516 62 16 2 12 72 52 108 2.12e-19 84.3
MsaG010571 AT3G55370.3 64.516 62 16 2 12 72 97 153 2.23e-19 84.7
MsaG010571 AT3G55370.2 64.516 62 16 2 12 72 52 108 2.54e-19 84.3
MsaG010571 AT3G61850.2 82.500 40 7 0 33 72 55 94 1.49e-18 81.6
MsaG010571 AT3G61850.3 82.500 40 7 0 33 72 55 94 1.49e-18 81.6
MsaG010571 AT3G61850.1 82.500 40 7 0 33 72 67 106 1.61e-18 81.6
MsaG010571 AT3G61850.4 82.500 40 7 0 33 72 67 106 1.79e-18 81.6
MsaG010571 AT3G61850.5 75.000 44 11 0 33 76 55 98 1.86e-18 81.6
MsaG010571 AT5G02460.1 82.051 39 7 0 34 72 89 127 1.60e-17 79.7
MsaG010571 AT2G37590.1 60.000 55 22 0 22 76 71 125 4.75e-17 77.8
MsaG010571 AT1G47655.1 68.889 45 14 0 32 76 21 65 1.44e-16 74.7
MsaG010571 AT1G07640.2 47.826 69 35 1 9 76 45 113 3.62e-16 75.5
MsaG010571 AT1G07640.3 47.826 69 35 1 9 76 53 121 4.19e-16 75.5
MsaG010571 AT4G00940.1 71.429 42 12 0 35 76 63 104 5.48e-16 74.7
MsaG010571 AT4G00940.3 71.429 42 12 0 35 76 63 104 5.48e-16 74.7
MsaG010571 AT4G00940.2 71.429 42 12 0 35 76 63 104 5.48e-16 74.7
MsaG010571 AT2G28810.2 78.378 37 8 0 36 72 73 109 6.14e-16 74.7
MsaG010571 AT5G65590.1 64.815 54 15 1 23 72 20 73 6.61e-16 74.7
MsaG010571 AT2G28810.1 78.378 37 8 0 36 72 90 126 7.05e-16 74.7
MsaG010571 AT4G38000.1 65.116 43 15 0 30 72 31 73 1.78e-15 72.4
MsaG010571 AT5G62940.1 67.442 43 14 0 30 72 63 105 2.44e-15 73.6
MsaG010571 AT2G28510.1 76.923 39 9 0 38 76 46 84 2.46e-15 72.8
MsaG010571 AT1G07640.1 57.895 57 23 1 21 76 1 57 2.48e-15 72.4
MsaG010571 AT5G66940.1 75.610 41 9 1 32 72 25 64 4.95e-15 70.9
MsaG010571 AT3G52440.1 72.500 40 11 0 37 76 22 61 5.77e-15 71.2
MsaG010571 AT3G52440.2 72.500 40 11 0 37 76 41 80 7.32e-15 71.2
MsaG010571 AT5G60850.1 80.000 35 7 0 38 72 49 83 1.31e-14 70.9
MsaG010571 AT1G51700.1 56.604 53 20 1 20 72 16 65 1.44e-14 69.3
MsaG010571 AT3G21270.1 81.818 33 6 0 40 72 29 61 6.04e-14 67.8
MsaG010571 AT1G21340.1 82.353 34 6 0 39 72 37 70 6.18e-14 68.6
MsaG010571 AT1G28310.1 78.788 33 7 0 40 72 27 59 1.08e-13 68.6
MsaG010571 AT1G28310.2 78.788 33 7 0 40 72 51 83 1.30e-13 68.2
MsaG010571 AT3G50410.1 69.444 36 11 0 37 72 27 62 2.21e-13 67.0
MsaG010571 AT1G26790.1 47.170 53 28 0 20 72 91 143 7.97e-13 66.2
MsaG010571 AT4G21050.1 67.500 40 11 1 33 72 19 56 8.56e-13 64.7
MsaG010571 AT5G62430.1 58.333 48 19 1 26 72 39 86 1.14e-12 65.5
MsaG010571 AT5G39660.3 54.902 51 19 1 22 72 124 170 1.26e-12 65.9
MsaG010571 AT5G39660.1 54.902 51 19 1 22 72 124 170 1.26e-12 65.9
MsaG010571 AT5G39660.2 54.902 51 19 1 22 72 124 170 1.26e-12 65.9
MsaG010571 AT1G69570.1 63.158 38 14 0 35 72 127 164 1.66e-12 65.5
MsaG010571 AT3G47500.1 63.158 38 14 0 35 72 105 142 3.25e-12 64.7
MsaG010571 AT2G34140.1 57.895 38 16 0 35 72 53 90 8.85e-12 61.2
MsaG010571 AT1G29160.1 57.895 38 16 0 35 72 57 94 1.45e-11 60.8
MsaG010571 AT4G21040.1 56.140 57 24 1 17 72 1 57 2.27e-11 61.2

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATAAACCATGGATTTA+TGG 0.149923 2:+75042872 MsaT010571.1:CDS
GCTTGTTCTGGTTGAGGTTT+TGG 0.170758 2:-75042468 None:intergenic
ATCCATAAATCCATGGTTTA+TGG 0.190178 2:-75042874 None:intergenic
CCAGAATCTTCCGTTGGATT+TGG 0.289703 2:-75045218 None:intergenic
ATTAGAAGTGGATTATTTCT+TGG 0.296220 2:+75042900 MsaT010571.1:CDS
GGACAGTTCAAAGCTTGTTC+TGG 0.303186 2:-75042480 None:intergenic
GTTGTTATAGTAACAGAATT+TGG 0.321643 2:-75042523 None:intergenic
GATCATGATGCTTCAAATCT+AGG 0.329150 2:+75042825 MsaT010571.1:CDS
GCTTCAAATCTAGGAAATTC+AGG 0.333482 2:+75042834 MsaT010571.1:CDS
AGTGGATTATTTCTTGGAAA+TGG 0.336042 2:+75042906 MsaT010571.1:CDS
ATGTATGGTGTGTATGGAAA+TGG 0.354795 2:+75042957 MsaT010571.1:CDS
CCAAATCCAACGGAAGATTC+TGG 0.360027 2:+75045218 MsaT010571.1:CDS
CAAGATTTGCAGAAATATCT+TGG 0.375372 2:-75042561 None:intergenic
ATTGTTAATTTCTTGATCAT+AGG 0.377525 2:-75043054 None:intergenic
AAAGCAATCATGTGGACAAA+TGG 0.379363 2:+75042794 MsaT010571.1:CDS
ATTTCTGCAAATCTTGTAGA+AGG 0.388906 2:+75042568 MsaT010571.1:CDS
TCATCAATGGAAAACATGTT+AGG 0.412061 2:+75042417 MsaT010571.1:CDS
TTGTTAATTTCTTGATCATA+GGG 0.429595 2:-75043053 None:intergenic
CAGAATATGTATGGTGTGTA+TGG 0.444454 2:+75042951 MsaT010571.1:CDS
TTCAAAGCTTGTTCTGGTTG+AGG 0.444926 2:-75042474 None:intergenic
TGTATGGTGTGTATGGAAAT+GGG 0.450678 2:+75042958 MsaT010571.1:CDS
AAGCAATCATGTGGACAAAT+GGG 0.452916 2:+75042795 MsaT010571.1:CDS
TCCTTCTTGTGGACAACCTC+AGG 0.458211 2:+75041571 MsaT010571.1:CDS
GTGTTGGTTGAGTTGCATCT+TGG 0.458480 2:-75042501 None:intergenic
AATAATGTTCAGAATATGTA+TGG 0.460682 2:+75042942 MsaT010571.1:CDS
ATGCAAATAACAATGCTTGT+GGG 0.493320 2:+75043009 MsaT010571.1:CDS
AATGTCGAGCCAATCATCAA+TGG 0.508256 2:+75042404 MsaT010571.1:CDS
GACAATGGTGAAGTAAATAG+TGG 0.512707 2:+75042981 MsaT010571.1:CDS
CTCACGCCAGAATCTTCCGT+TGG 0.513191 2:-75045224 None:intergenic
TTTATGGATGCAATTAGAAG+TGG 0.515360 2:+75042888 MsaT010571.1:CDS
ATAGTAACAGAATTTGGTGT+TGG 0.529356 2:-75042517 None:intergenic
TAATTGCATCCATAAATCCA+TGG 0.534717 2:-75042881 None:intergenic
GTTTGGATGGATCCTTCTTG+TGG 0.537133 2:+75041560 None:intergenic
AATGCAAATAACAATGCTTG+TGG 0.543604 2:+75043008 MsaT010571.1:CDS
AGCAAGAAATGTGCAGTGGA+AGG 0.565157 2:+75043081 MsaT010571.1:CDS
ACCTGAGGTTGTCCACAAGA+AGG 0.573402 2:-75041572 None:intergenic
TGCAAATAACAATGCTTGTG+GGG 0.582187 2:+75043010 MsaT010571.1:CDS
GCAAGAAATGTGCAGTGGAA+GGG 0.598256 2:+75043082 MsaT010571.1:CDS
GTGTATGGAAATGGGGACAA+TGG 0.598902 2:+75042966 MsaT010571.1:CDS
AATCATTCAACCATAAACCA+TGG 0.606118 2:+75042864 MsaT010571.1:CDS
GTATGGTGTGTATGGAAATG+GGG 0.629075 2:+75042959 MsaT010571.1:CDS
AGGCTTCAAAAGCAATCATG+TGG 0.635965 2:+75042786 MsaT010571.1:intron
GTGAAGCAAGAAATGTGCAG+TGG 0.683660 2:+75043077 MsaT010571.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 75041566 75045245 75041566 ID=MsaG010571
Chr2 mRNA 75041566 75045245 75041566 ID=MsaT010571.1;Parent=MsaG010571
Chr2 exon 75041566 75041592 75041566 ID=MsaT010571.1.exon1;Parent=MsaT010571.1
Chr2 CDS 75041566 75041592 75041566 ID=cds.MsaT010571.1;Parent=MsaT010571.1
Chr2 exon 75042402 75042589 75042402 ID=MsaT010571.1.exon2;Parent=MsaT010571.1
Chr2 CDS 75042402 75042589 75042402 ID=cds.MsaT010571.1;Parent=MsaT010571.1
Chr2 exon 75042788 75043119 75042788 ID=MsaT010571.1.exon3;Parent=MsaT010571.1
Chr2 CDS 75042788 75043119 75042788 ID=cds.MsaT010571.1;Parent=MsaT010571.1
Chr2 exon 75045217 75045245 75045217 ID=MsaT010571.1.exon4;Parent=MsaT010571.1
Chr2 CDS 75045217 75045245 75045217 ID=cds.MsaT010571.1;Parent=MsaT010571.1
Gene Sequence

>MsaT010571.1

ATGGATCCTTCTTGTGGACAACCTCAGCAAATGTCGAGCCAATCATCAATGGAAAACATGTTAGGTTGCTCAAAAGATGAGCAAGAAAGAAAGCCAAAACCTCAACCAGAACAAGCTTTGAACTGTCCAAGATGCAACTCAACCAACACCAAATTCTGTTACTATAACAACTATAGTCTTACTCAGCCAAGATATTTCTGCAAATCTTGTAGAAGGCTTCAAAAGCAATCATGTGGACAAATGGGTTATGATGATCATGATGCTTCAAATCTAGGAAATTCAGGTATGAACAATCATTCAACCATAAACCATGGATTTATGGATGCAATTAGAAGTGGATTATTTCTTGGAAATGGACTACATTATAATAATAATGTTCAGAATATGTATGGTGTGTATGGAAATGGGGACAATGGTGAAGTAAATAGTGGTAACAATGCAAATAACAATGCTTGTGGGGTTAGTGAAGAAATGATGTTTCCCTATGATCAAGAAATTAACAATGTGAAGCAAGAAATGTGCAGTGGAAGGGAACAAATTAGTGAAAACCAAATCCAACGGAAGATTCTGGCGTGA
Protein sequence

>MsaT010571.1

MDPSCGQPQQMSSQSSMENMLGCSKDEQERKPKPQPEQALNCPRCNSTNTKFCYYNNYSLTQPRYFCKSCRRLQKQSCGQMGYDDHDASNLGNSGMNNHSTINHGFMDAIRSGLFLGNGLHYNNNVQNMYGVYGNGDNGEVNSGNNANNNACGVSEEMMFPYDQEINNVKQEMCSGREQISENQIQRKILA*