AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010586

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010586 MtrunA17_Chr2g0326281 91.252 823 52 3 1 803 1 823 0.0 1504
MsaG010586 MtrunA17_Chr4g0029671 71.917 819 187 13 1 803 1 792 0.0 1111
MsaG010586 MtrunA17_Chr4g0046371 42.114 634 322 14 51 660 33 645 3.13e-143 438
MsaG010586 MtrunA17_Chr2g0282961 53.808 407 159 9 55 448 52 442 1.01e-134 413
MsaG010586 MtrunA17_Chr8g0385791 46.121 464 224 9 14 466 21 469 1.75e-128 404
MsaG010586 MtrunA17_Chr8g0385791 50.000 104 52 0 670 773 705 808 3.87e-30 127
MsaG010586 MtrunA17_Chr2g0276781 49.870 385 168 6 36 412 7 374 7.20e-119 374
MsaG010586 MtrunA17_Chr2g0276781 46.212 132 67 2 665 792 537 668 5.86e-28 120
MsaG010586 MtrunA17_Chr5g0433301 47.901 405 170 11 56 446 24 401 1.06e-107 349
MsaG010586 MtrunA17_Chr5g0433301 36.691 139 80 4 628 763 670 803 4.71e-19 92.4
MsaG010586 MtrunA17_Chr8g0346711 48.361 366 169 8 54 412 27 379 4.34e-107 343
MsaG010586 MtrunA17_Chr8g0346711 51.579 95 45 1 675 769 558 651 5.82e-24 107
MsaG010586 MtrunA17_Chr3g0109391 51.404 356 145 8 56 398 26 366 1.02e-105 344
MsaG010586 MtrunA17_Chr3g0109391 41.880 117 59 3 655 763 696 811 6.47e-19 92.0
MsaG010586 MtrunA17_Chr2g0285461 50.275 364 161 7 56 410 23 375 6.96e-105 343
MsaG010586 MtrunA17_Chr2g0285461 40.152 132 73 4 642 769 743 872 7.08e-21 98.6
MsaG010586 MtrunA17_Chr4g0009751 48.127 347 162 5 56 396 19 353 2.55e-103 331
MsaG010586 MtrunA17_Chr4g0009751 47.619 84 40 2 659 742 542 621 1.90e-15 80.5
MsaG010586 MtrunA17_Chr8g0376771 49.311 363 165 6 56 410 23 374 4.72e-103 338
MsaG010586 MtrunA17_Chr8g0376771 38.095 126 74 3 641 763 745 869 1.76e-19 94.0
MsaG010586 MtrunA17_Chr7g0239061 44.186 387 196 8 54 433 12 385 2.48e-102 330
MsaG010586 MtrunA17_Chr7g0239061 48.148 108 52 2 659 766 557 660 4.17e-24 108
MsaG010586 MtrunA17_Chr1g0156321 50.712 351 152 7 56 397 43 381 7.82e-101 331
MsaG010586 MtrunA17_Chr1g0156321 43.269 104 57 2 675 777 754 856 3.50e-18 89.7
MsaG010586 MtrunA17_Chr4g0070181 51.014 345 151 5 56 393 23 356 8.86e-96 323
MsaG010586 MtrunA17_Chr4g0070181 49.485 97 47 2 670 765 989 1084 1.59e-22 103
MsaG010586 MtrunA17_Chr2g0301251 49.565 345 156 5 56 393 23 356 1.20e-93 317
MsaG010586 MtrunA17_Chr2g0301251 49.485 97 47 2 670 765 981 1076 1.73e-21 100
MsaG010586 MtrunA17_Chr8g0389081 46.499 357 157 7 56 396 32 370 1.18e-85 294
MsaG010586 MtrunA17_Chr8g0389081 48.913 92 45 2 675 765 985 1075 5.79e-21 99.0
MsaG010586 MtrunA17_Chr2g0326761 40.420 381 167 10 56 392 11 375 8.95e-74 254
MsaG010586 MtrunA17_Chr1g0181211 36.145 415 201 9 56 422 27 425 1.64e-69 242
MsaG010586 MtrunA17_Chr1g0199681 36.434 387 193 6 56 402 94 467 4.48e-67 236
MsaG010586 MtrunA17_Chr4g0028971 37.280 397 183 8 56 402 12 392 2.62e-66 234
MsaG010586 MtrunA17_Chr7g0264171 35.437 412 196 10 56 412 31 427 2.74e-61 218
MsaG010586 MtrunA17_Chr7g0264161 37.778 315 175 7 65 372 1 301 5.76e-56 194
MsaG010586 MtrunA17_Chr3g0115271 35.038 391 194 12 56 400 19 395 1.61e-53 196
MsaG010586 MtrunA17_Chr8g0356211 41.048 229 121 4 176 396 2 224 6.98e-53 186
MsaG010586 MtrunA17_Chr5g0417281 33.696 368 204 13 56 410 11 351 1.22e-50 186
MsaG010586 MtrunA17_Chr5g0424931 30.900 411 224 12 36 409 2 389 8.36e-50 184
MsaG010586 MtrunA17_Chr5g0417211 34.270 356 199 12 56 404 12 339 2.84e-46 171
MsaG010586 MtrunA17_Chr5g0436761 34.501 371 206 12 56 414 12 357 3.83e-46 171
MsaG010586 MtrunA17_Chr2g0289221 33.333 333 175 9 56 372 20 321 7.48e-43 159
MsaG010586 MtrunA17_Chr1g0178011 28.032 371 233 7 56 414 12 360 8.97e-41 156
MsaG010586 MtrunA17_Chr2g0277461 29.091 385 242 9 56 431 13 375 8.48e-39 150
MsaG010586 MtrunA17_Chr5g0424741 28.982 383 226 13 56 402 12 384 2.77e-34 138
MsaG010586 MtrunA17_Chr5g0425661 33.014 209 128 3 56 264 37 233 2.20e-32 125
MsaG010586 MtrunA17_Chr2g0277531 35.586 222 127 8 51 268 15 224 4.14e-32 127
MsaG010586 MtrunA17_Chr5g0424801 28.011 357 218 11 56 396 12 345 1.65e-26 112
MsaG010586 MtrunA17_Chr5g0425261 27.887 355 221 11 56 396 12 345 4.53e-25 108
MsaG010586 MtrunA17_Chr1g0156301 36.667 90 41 2 675 764 177 250 3.48e-11 64.7
MsaG010586 MtrunA17_Chr7g0276471 36.111 108 68 1 166 273 116 222 3.56e-11 66.2
MsaG010586 MtrunA17_Chr8g0345771 36.697 109 67 2 154 262 158 264 7.14e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010586 AT5G60450.2 57.236 767 278 13 50 803 35 764 0.0 802
MsaG010586 AT5G60450.1 57.274 763 276 13 54 803 63 788 0.0 799
MsaG010586 AT5G62000.2 36.779 832 417 21 14 769 29 827 9.03e-165 499
MsaG010586 AT5G62000.5 36.779 832 417 21 14 769 29 827 9.03e-165 499
MsaG010586 AT5G62000.3 36.779 832 417 21 14 769 29 827 9.03e-165 499
MsaG010586 AT5G62000.1 36.779 832 417 21 14 769 29 827 9.03e-165 499
MsaG010586 AT5G62000.4 36.779 832 417 21 14 769 29 827 1.40e-164 499
MsaG010586 AT2G33860.1 49.272 481 189 11 55 523 53 490 9.43e-142 431
MsaG010586 AT3G61830.1 45.939 394 188 8 43 424 12 392 8.93e-113 356
MsaG010586 AT3G61830.1 49.020 102 51 1 666 767 480 580 2.70e-24 108
MsaG010586 AT1G59750.4 48.361 366 168 6 56 412 22 375 9.07e-110 350
MsaG010586 AT1G59750.4 56.000 100 44 0 665 764 527 626 3.20e-29 124
MsaG010586 AT1G59750.2 48.361 366 168 6 56 412 22 375 1.06e-109 350
MsaG010586 AT1G59750.2 56.000 100 44 0 665 764 529 628 3.34e-29 124
MsaG010586 AT1G59750.1 48.361 366 168 6 56 412 22 375 1.51e-109 349
MsaG010586 AT1G59750.1 56.000 100 44 0 665 764 532 631 3.42e-29 124
MsaG010586 AT1G59750.3 48.361 366 168 6 56 412 22 375 1.51e-109 349
MsaG010586 AT1G59750.3 56.000 100 44 0 665 764 532 631 3.42e-29 124
MsaG010586 AT2G33860.2 49.587 363 147 6 167 523 1 333 4.71e-107 336
MsaG010586 AT5G37020.2 50.275 364 161 7 56 410 22 374 4.94e-106 343
MsaG010586 AT5G37020.2 52.174 69 31 2 675 742 705 772 2.53e-16 84.0
MsaG010586 AT2G46530.1 46.049 367 177 8 54 412 19 372 6.53e-106 338
MsaG010586 AT2G46530.1 44.000 125 65 2 639 763 458 577 3.46e-25 111
MsaG010586 AT5G37020.1 50.136 367 157 8 56 410 22 374 1.20e-105 343
MsaG010586 AT5G37020.1 47.778 90 45 2 675 763 705 793 1.80e-19 94.4
MsaG010586 AT2G46530.3 46.049 367 177 8 54 412 40 393 1.34e-105 338
MsaG010586 AT2G46530.3 44.000 125 65 2 639 763 479 598 4.50e-25 111
MsaG010586 AT1G30330.3 47.938 388 168 8 56 420 25 401 4.93e-103 339
MsaG010586 AT1G30330.3 46.078 102 51 3 665 763 784 884 8.35e-21 98.6
MsaG010586 AT1G30330.2 47.938 388 168 8 56 420 25 401 4.93e-103 339
MsaG010586 AT1G30330.2 46.078 102 51 3 665 763 784 884 8.35e-21 98.6
MsaG010586 AT1G30330.1 47.938 388 168 8 56 420 23 399 5.10e-103 339
MsaG010586 AT1G30330.1 46.078 102 51 3 665 763 782 882 8.41e-21 98.6
MsaG010586 AT4G23980.2 48.424 349 156 8 54 393 10 343 6.56e-100 323
MsaG010586 AT4G23980.2 53.000 100 46 1 667 766 513 611 2.97e-26 115
MsaG010586 AT4G23980.1 48.424 349 156 8 54 393 10 343 7.20e-100 323
MsaG010586 AT4G23980.1 53.000 100 46 1 667 766 515 613 3.10e-26 115
MsaG010586 AT3G61830.2 45.481 343 164 6 92 424 12 341 1.22e-97 315
MsaG010586 AT3G61830.2 49.020 102 51 1 666 767 429 529 1.77e-24 108
MsaG010586 AT1G19850.2 49.148 352 157 8 56 397 43 382 6.27e-96 319
MsaG010586 AT1G19850.2 45.455 99 52 2 667 764 774 871 8.44e-19 92.0
MsaG010586 AT1G19850.1 49.148 352 157 8 56 397 54 393 6.69e-96 319
MsaG010586 AT1G19850.1 45.455 99 52 2 667 764 785 882 8.66e-19 92.0
MsaG010586 AT1G19220.1 50.432 347 150 6 56 393 23 356 1.03e-93 317
MsaG010586 AT1G19220.1 46.903 113 54 3 670 777 953 1064 6.03e-23 105
MsaG010586 AT5G20730.2 49.568 347 153 6 56 393 24 357 1.35e-90 310
MsaG010586 AT5G20730.2 38.182 165 78 6 604 765 984 1127 5.83e-22 102
MsaG010586 AT5G20730.3 49.425 348 153 6 56 393 24 358 1.36e-90 309
MsaG010586 AT5G20730.3 38.182 165 78 6 604 765 985 1128 5.27e-22 102
MsaG010586 AT5G20730.1 49.425 348 153 6 56 393 24 358 1.84e-90 309
MsaG010586 AT5G20730.1 38.182 165 78 6 604 765 985 1128 5.64e-22 102
MsaG010586 AT1G35540.1 40.761 368 195 6 51 413 22 371 1.24e-85 284
MsaG010586 AT1G35540.1 30.268 261 126 7 509 763 387 597 4.18e-24 108
MsaG010586 AT1G34410.1 39.459 370 198 8 51 411 22 374 1.79e-82 276
MsaG010586 AT1G34410.1 53.684 95 43 1 667 761 503 596 1.55e-23 106
MsaG010586 AT1G34310.1 39.837 369 198 7 51 411 22 374 2.69e-82 275
MsaG010586 AT1G34310.1 48.624 109 54 2 647 754 482 589 7.56e-23 104
MsaG010586 AT1G35240.1 40.327 367 198 6 51 412 22 372 4.99e-82 274
MsaG010586 AT1G35240.1 52.632 95 44 1 667 761 489 582 3.88e-23 105
MsaG010586 AT5G37020.3 53.101 258 113 3 160 410 23 279 3.81e-81 275
MsaG010586 AT5G37020.3 47.778 90 45 2 675 763 610 698 1.35e-19 94.4
MsaG010586 AT1G34170.1 39.353 371 199 8 54 417 23 374 7.77e-81 268
MsaG010586 AT1G35520.1 40.327 367 196 7 51 412 22 370 4.88e-80 269
MsaG010586 AT1G35520.1 46.789 109 56 2 647 754 482 589 8.43e-22 100
MsaG010586 AT1G34390.1 40.576 382 199 10 51 424 22 383 1.62e-78 265
MsaG010586 AT1G34390.1 46.364 110 57 2 647 755 480 588 5.73e-21 98.6
MsaG010586 AT2G46530.2 46.718 259 130 4 160 412 29 285 9.64e-77 258
MsaG010586 AT2G46530.2 44.000 125 65 2 639 763 371 490 1.65e-25 112
MsaG010586 AT2G28350.2 37.349 415 199 6 56 422 10 411 3.30e-75 258
MsaG010586 AT2G28350.1 37.349 415 199 6 56 422 10 411 5.37e-75 258
MsaG010586 AT4G30080.1 37.596 391 188 8 56 404 19 395 1.30e-72 251
MsaG010586 AT1G34170.2 37.330 367 186 8 54 417 23 348 3.56e-70 239
MsaG010586 AT1G34170.3 37.330 367 186 8 54 417 23 348 1.85e-69 239
MsaG010586 AT1G34170.3 40.566 106 55 2 667 765 418 522 2.68e-18 89.7
MsaG010586 AT1G77850.2 29.046 482 274 11 57 496 20 475 4.97e-51 189
MsaG010586 AT1G77850.1 29.046 482 274 11 57 496 20 475 5.34e-51 189
MsaG010586 AT1G43950.1 44.037 218 104 5 51 267 22 222 7.21e-49 172
MsaG010586 AT4G01500.1 38.043 92 54 1 166 257 34 122 9.76e-12 67.8
MsaG010586 AT1G01030.2 36.735 98 61 1 160 257 48 144 1.13e-11 67.4
MsaG010586 AT2G46870.1 34.328 134 84 3 143 273 10 142 1.15e-11 67.0
MsaG010586 AT1G01030.1 36.735 98 61 1 160 257 48 144 1.47e-11 67.4
MsaG010586 AT1G13260.1 34.711 121 75 2 149 265 167 287 3.05e-11 66.2

Find 187 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATAGCTGACCTTCTTTC+TGG 0.139715 2:+75257155 None:intergenic
GTAAATCCTTGTAGACTATT+TGG 0.158429 2:-75255879 MsaT010586.1:CDS
AACCTCATCATTCTCCTTAT+TGG 0.160497 2:+75258755 None:intergenic
CTTCTTCTTGTGTATATATA+TGG 0.165929 2:+75255059 None:intergenic
AGCCAAAAGAATCTTGTTTC+TGG 0.202166 2:-75257688 MsaT010586.1:CDS
AACATTCTCTAGTTGGAAAT+TGG 0.212031 2:+75256064 None:intergenic
AGAAATGAAGTTGGAATATC+AGG 0.255023 2:-75256005 MsaT010586.1:CDS
TCATTGGAAATTCCAACTTA+TGG 0.267049 2:-75258943 MsaT010586.1:CDS
CTTCATCTTATCTTGAACTA+TGG 0.268353 2:-75259071 MsaT010586.1:CDS
AGCTGGAGACAGTGGGTTTA+TGG 0.268758 2:-75256549 MsaT010586.1:CDS
ACTACAACATTCCCTTTCTT+TGG 0.271823 2:+75259018 None:intergenic
TTCTTTGGAAGTGATGTTAA+AGG 0.285859 2:+75259033 None:intergenic
CCTGCAGCTTCTGCTTGTTT+CGG 0.298110 2:+75255973 None:intergenic
TTTGACTTGGGCCATTTATA+GGG 0.298149 2:+75257027 None:intergenic
CCCCAGAAACAAGATTCTTT+TGG 0.320379 2:+75257686 None:intergenic
AAACTTAACTTTCACTCATT+AGG 0.322689 2:-75256173 MsaT010586.1:CDS
AGTGAACTAGAGAGACTATT+TGG 0.331029 2:-75255618 MsaT010586.1:CDS
GTGATTTGGATCCCTATAAA+TGG 0.335220 2:-75257038 MsaT010586.1:CDS
TTTCCATTAACAGGCCAATA+AGG 0.336836 2:-75258769 MsaT010586.1:intron
GAAGTGGAGAAGATGACAAT+TGG 0.348700 2:-75255040 MsaT010586.1:CDS
CGGAAACGAACCGGTTTCCT+AGG 0.350274 2:-75256316 MsaT010586.1:CDS
TAGTTGGAAATTGGTTGGTT+TGG 0.350673 2:+75256073 None:intergenic
TGGTTACCAACAATTGATTC+AGG 0.358579 2:+75257471 None:intergenic
TTCACCTACCTCGATAAATA+TGG 0.367329 2:+75257835 None:intergenic
CTGCAGTGAGTTCTGTGTTA+TGG 0.372350 2:-75255092 MsaT010586.1:intron
ATTCAAAATGAAATTTGAAA+TGG 0.374517 2:-75257186 MsaT010586.1:CDS
TTGATCTAAACCTTAACTCT+TGG 0.379579 2:-75256244 MsaT010586.1:CDS
CCACCTGCTTCTAAACCTTC+AGG 0.382333 2:+75258289 None:intergenic
ATAAATGGCCCAAGTCAAAA+TGG 0.382489 2:-75257023 MsaT010586.1:CDS
GCCAAAAGAATCTTGTTTCT+GGG 0.385570 2:-75257687 MsaT010586.1:CDS
GGGACGCAGTGCTCTTTCTA+AGG 0.386238 2:-75257666 MsaT010586.1:intron
TGATCTAAACCTTAACTCTT+GGG 0.391011 2:-75256243 MsaT010586.1:CDS
TAAATGAAACATTCTCTAGT+TGG 0.401148 2:+75256057 None:intergenic
CACAAACAAGGGAGCTTAGT+TGG 0.402834 2:-75255687 MsaT010586.1:CDS
ATGTGAGAAGCTAACTTTGT+AGG 0.405019 2:+75258247 None:intergenic
TGACAGTGAAAATGACATTA+TGG 0.405346 2:-75255544 MsaT010586.1:CDS
TCAGTTAGCAGGGATGTGTT+TGG 0.410443 2:-75258323 MsaT010586.1:intron
GGTTTAGAAGCAGGTGGAGA+GGG 0.411642 2:-75258283 MsaT010586.1:CDS
ATTTCTGCTGCTGCTTCTCT+AGG 0.415866 2:-75255945 MsaT010586.1:CDS
TTGGTAAGGCACAACGAAAT+CGG 0.417321 2:+75257253 None:intergenic
CATACTAATCTTGCATCAAA+TGG 0.420961 2:-75256401 MsaT010586.1:CDS
TAATGAGTGAAAGTTAAGTT+TGG 0.421167 2:+75256175 None:intergenic
GATTCAGGAAGACCGTTTCT+CGG 0.422054 2:+75257486 None:intergenic
TTAACATCACTTCCAAAGAA+AGG 0.425871 2:-75259030 MsaT010586.1:CDS
TGATGATAACCAGAGTTGTT+TGG 0.426706 2:-75255008 MsaT010586.1:CDS
TTGTGTTGCATTCAGTTAGC+AGG 0.431661 2:-75258334 MsaT010586.1:intron
GTCAAAATGGAGATGCTTGA+TGG 0.432058 2:-75257010 MsaT010586.1:intron
AAAATGACATTATGGTTGTT+GGG 0.432406 2:-75255536 MsaT010586.1:CDS
AAGTGGAGAAGATGACAATT+GGG 0.436343 2:-75255039 MsaT010586.1:CDS
GAACTATGGCATTCTTGTGC+TGG 0.437430 2:-75259057 MsaT010586.1:CDS
AATACTCCTTCAGCTCAGAT+AGG 0.438938 2:-75256035 MsaT010586.1:CDS
TTCTAGTTATGCAGACTTCA+CGG 0.440375 2:-75256336 MsaT010586.1:CDS
CAGACTTCACGGAAACGAAC+CGG 0.444346 2:-75256325 MsaT010586.1:CDS
ACTACTGTTGGAGAAGAATC+TGG 0.448069 2:+75254935 None:intergenic
TTAGATCAACCTTTCCTGCC+AGG 0.450136 2:+75256258 None:intergenic
AGTGACTGGAATGAGTGATT+TGG 0.450736 2:-75257052 MsaT010586.1:CDS
TTCTTGACCTTGCAAGACCT+TGG 0.450899 2:+75256502 None:intergenic
TCGACAAGATTGAATGTTCA+AGG 0.452440 2:+75256710 None:intergenic
ATCAAGATAGAGTCTCTCCT+TGG 0.455503 2:-75256761 MsaT010586.1:CDS
GGTCTTCCTGAATCAATTGT+TGG 0.457519 2:-75257477 MsaT010586.1:CDS
TTGGATTCATACCAGTGATA+AGG 0.459048 2:+75256640 None:intergenic
GCCTCTGATACCAGCACTCA+TGG 0.459057 2:-75258205 MsaT010586.1:CDS
GTGAAGTCTGCATAACTAGA+AGG 0.460590 2:+75256338 None:intergenic
AAAAGTATGCTAAAAGCATC+AGG 0.463277 2:-75257232 MsaT010586.1:CDS
TAAGTTTGGTTGCATTATGC+AGG 0.464035 2:+75256189 None:intergenic
GAAGCAGGTGGAGAGGGAGA+TGG 0.465289 2:-75258277 MsaT010586.1:CDS
ACAACCCTACAAAAGATTTG+TGG 0.468787 2:+75258913 None:intergenic
GGTGGTTTCCGCCGCCGATA+AGG 0.472069 2:+75255851 None:intergenic
TAACATCACTTCCAAAGAAA+GGG 0.474815 2:-75259029 MsaT010586.1:CDS
GTAGTGAGCAGATGCCTCCT+TGG 0.475837 2:+75257730 None:intergenic
TTCTCTAGTTGGAAATTGGT+TGG 0.476906 2:+75256068 None:intergenic
ACTTTGTGTATATCACCATA+TGG 0.477775 2:+75256122 None:intergenic
GAAAATGACATTATGGTTGT+TGG 0.484036 2:-75255537 MsaT010586.1:CDS
CTTGCAAGACCCTAGGAAAC+CGG 0.485163 2:+75256306 None:intergenic
TTTGAAGCCTCCACAATCAA+TGG 0.488117 2:+75254977 None:intergenic
TCCTAGTTTCCTTTCTTCAG+TGG 0.491250 2:-75257439 MsaT010586.1:CDS
TTTAGTAAAACTTACTGCCA+TGG 0.494050 2:+75255507 None:intergenic
TACTTTGCTTCCACAGGCAG+AGG 0.504284 2:-75258721 MsaT010586.1:intron
AGGAGGCATCTGCTCACTAC+TGG 0.505121 2:-75257727 MsaT010586.1:CDS
CATACCAGTGATAAGGTGAC+TGG 0.506398 2:+75256647 None:intergenic
GGCATCTGCTCACTACTGGC+TGG 0.509364 2:-75257723 MsaT010586.1:CDS
TAAGAGATCCTGACAAAGGA+TGG 0.509914 2:-75255581 MsaT010586.1:CDS
AACTAGATTCTAACTACTGT+TGG 0.511297 2:+75254923 None:intergenic
TATATCACCATATGGAAAAG+AGG 0.512064 2:+75256130 None:intergenic
ATCTTGTCCCTATGGTCAGT+GGG 0.513478 2:+75257207 None:intergenic
TGTGGTTGAAGACCATAAGT+TGG 0.513554 2:+75258931 None:intergenic
GAGCCTGAAGGTTTAGAAGC+AGG 0.515631 2:-75258292 MsaT010586.1:CDS
GTGATATTACACAGGTCCGA+TGG 0.515735 2:-75256806 MsaT010586.1:intron
ATTCTTCTTAACTTGTGTTG+TGG 0.515801 2:+75259200 None:intergenic
ATAATTTGTGTAACTAACAT+TGG 0.517422 2:+75256214 None:intergenic
AATCTTGTCCCTATGGTCAG+TGG 0.518063 2:+75257206 None:intergenic
ATTGAGCAGCTACAATGATC+TGG 0.518918 2:-75255643 MsaT010586.1:CDS
TGAAAACGGAGAGTTAAGAT+TGG 0.519467 2:-75257529 MsaT010586.1:CDS
AGAGCTGCTCGACCGAGAAA+CGG 0.520377 2:-75257498 MsaT010586.1:CDS
CTTCTCTAGGTGCAAACTTG+AGG 0.522379 2:-75255932 MsaT010586.1:CDS
ATTGAGATCCCTGGCAGGAA+AGG 0.523346 2:-75256267 MsaT010586.1:CDS
TCTTGACCTTGCAAGACCCT+AGG 0.523482 2:+75256299 None:intergenic
GGTGCATTGAGAAATGAAGT+TGG 0.523787 2:-75256014 MsaT010586.1:CDS
TATATATACACAAGAAGAAG+TGG 0.530321 2:-75255056 MsaT010586.1:CDS
TCTCTATGTAGGGGTGAAAA+CGG 0.532577 2:-75257543 MsaT010586.1:intron
TCAAGATAGAGTCTCTCCTT+GGG 0.533378 2:-75256760 MsaT010586.1:CDS
TTCATCTTCTAGTTCCACTT+CGG 0.534499 2:-75259103 MsaT010586.1:CDS
TGAATGAAGGTTCACAAACA+AGG 0.534840 2:-75255699 MsaT010586.1:intron
TCAGGAACCCACTGACCATA+GGG 0.535798 2:-75257214 MsaT010586.1:CDS
TAGCATTAGCCACTGAAGAA+AGG 0.540509 2:+75257430 None:intergenic
CCGAAACAAGCAGAAGCTGC+AGG 0.542656 2:-75255973 MsaT010586.1:CDS
ACAGGTTACTTTGCTTCCAC+AGG 0.543725 2:-75258727 MsaT010586.1:CDS
TAGATCAACCTTTCCTGCCA+GGG 0.544208 2:+75256259 None:intergenic
TGTAGTAGCGGTATAGTGAC+TGG 0.545183 2:-75257066 MsaT010586.1:CDS
TATGGTGATATACACAAAGT+TGG 0.548146 2:-75256119 MsaT010586.1:CDS
GAAAACGGAGAGTTAAGATT+GGG 0.548310 2:-75257528 MsaT010586.1:CDS
TGGATGAATCTCCAGAAAGA+AGG 0.548781 2:-75257166 MsaT010586.1:intron
TTTCCTGCCAGGGATCTCAA+TGG 0.549270 2:+75256269 None:intergenic
GAGAGACTATTTGGCATGGA+AGG 0.550692 2:-75255609 MsaT010586.1:CDS
GCTCAATCTTGAGAGATCAA+TGG 0.551608 2:+75255659 None:intergenic
TGAAGAAACAAGAGCCGAAG+TGG 0.552863 2:+75259089 None:intergenic
AATATATCCATTGAGATCCC+TGG 0.553252 2:-75256276 MsaT010586.1:CDS
GGAACATACACCATTGATTG+TGG 0.553625 2:-75254987 MsaT010586.1:CDS
AGGCTGAAGAAACTGCGGAC+GGG 0.554562 2:-75256687 MsaT010586.1:CDS
TACACAGGTCCGATGGGATG+AGG 0.555224 2:-75256799 MsaT010586.1:intron
GGAAACTAGGAGAACAACTC+TGG 0.556326 2:+75257451 None:intergenic
TGAACATTCAATCTTGTCGA+AGG 0.558866 2:-75256707 MsaT010586.1:CDS
AGGCTGAACCCTCGTAAACT+CGG 0.559216 2:+75256358 None:intergenic
AGAAAACCCTCCATGAGTGC+TGG 0.560225 2:+75258195 None:intergenic
AAGAAGAATATATGTTGCAA+TGG 0.561906 2:-75259186 MsaT010586.1:CDS
TGTGTTGCATTCAGTTAGCA+GGG 0.565461 2:-75258333 MsaT010586.1:intron
GAAAATCCAAATAGTCTACA+AGG 0.566058 2:+75255873 None:intergenic
GCTTCAAAGTCTTCCTCAGT+GGG 0.567657 2:-75254962 MsaT010586.1:CDS
CCAAAAGAATCTTGTTTCTG+GGG 0.569533 2:-75257686 MsaT010586.1:CDS
TGATATTACACAGGTCCGAT+GGG 0.571325 2:-75256805 MsaT010586.1:intron
CCGATGGGATGAGGATATTG+AGG 0.572780 2:-75256790 MsaT010586.1:CDS
GCCACTGAAGAAAGGAAACT+AGG 0.575196 2:+75257438 None:intergenic
CGACAAGATTGAATGTTCAA+GGG 0.577787 2:+75256711 None:intergenic
GTGTATGTTCCAAACAACTC+TGG 0.578457 2:+75254999 None:intergenic
TGGTTGTTGGGGATGATCCA+TGG 0.581281 2:-75255524 MsaT010586.1:CDS
TCACCCAGTCACCTTATCAC+TGG 0.584443 2:-75256651 MsaT010586.1:intron
ATCAGGAACCCACTGACCAT+AGG 0.589785 2:-75257215 MsaT010586.1:CDS
TATCCATTGAGATCCCTGGC+AGG 0.592828 2:-75256272 MsaT010586.1:CDS
TTCATCGCAGCTGGAGACAG+TGG 0.594260 2:-75256557 MsaT010586.1:intron
AGGTTTAGAAGCAGGTGGAG+AGG 0.594537 2:-75258284 MsaT010586.1:CDS
ATTTCCACTCCACACCATGC+AGG 0.596067 2:+75257862 None:intergenic
TCATCGCAGCTGGAGACAGT+GGG 0.597361 2:-75256556 MsaT010586.1:intron
AATGCACCTATCTGAGCTGA+AGG 0.597701 2:+75256029 None:intergenic
CGGTTTCCTAGGGTCTTGCA+AGG 0.598452 2:-75256305 MsaT010586.1:CDS
AATCTTTGTCAGGTCAGCCA+AGG 0.599067 2:-75257747 MsaT010586.1:intron
AAGATTGAATGTTCAAGGGA+GGG 0.602810 2:+75256715 None:intergenic
TCCATGAGTGCTGGTATCAG+AGG 0.602998 2:+75258204 None:intergenic
TTACTTCTTCCGAGTTTACG+AGG 0.603395 2:-75256367 MsaT010586.1:CDS
GATATACACAAAGTTGGTCA+AGG 0.608483 2:-75256113 MsaT010586.1:CDS
GATCTAAACCTTAACTCTTG+GGG 0.609198 2:-75256242 MsaT010586.1:CDS
AAATGACATTATGGTTGTTG+GGG 0.611574 2:-75255535 MsaT010586.1:CDS
TTTGCAACAAGCTCTTGAGA+GGG 0.613596 2:+75257888 None:intergenic
GGCTTCAAAGTCTTCCTCAG+TGG 0.617874 2:-75254963 MsaT010586.1:CDS
GGAAACGAACCGGTTTCCTA+GGG 0.618178 2:-75256315 MsaT010586.1:CDS
AGATCGACCAAGGTCTTGCA+AGG 0.618905 2:-75256509 MsaT010586.1:CDS
TGTCATACTCATACCAAAGG+AGG 0.620459 2:+75258142 None:intergenic
CTGATACCAGCACTCATGGA+GGG 0.625106 2:-75258201 MsaT010586.1:CDS
CCTCAATATCCTCATCCCAT+CGG 0.625293 2:+75256790 None:intergenic
ATAAAACCTACCTCTGCCTG+TGG 0.625761 2:+75258711 None:intergenic
CTTTGTCAGGTCAGCCAAGG+AGG 0.625815 2:-75257744 MsaT010586.1:intron
ACTTGTTCCAAATGACCTTG+TGG 0.628653 2:+75258988 None:intergenic
TTGGATGACAAAGAGCCTGA+AGG 0.629150 2:-75258304 MsaT010586.1:CDS
GAATGAAGGTTCACAAACAA+GGG 0.629502 2:-75255698 MsaT010586.1:intron
CAAGATTGAATGTTCAAGGG+AGG 0.630768 2:+75256714 None:intergenic
ACTAGAGAGACTATTTGGCA+TGG 0.631155 2:-75255613 MsaT010586.1:CDS
TACTTCTTCCGAGTTTACGA+GGG 0.633533 2:-75256366 MsaT010586.1:CDS
CCTGAAGGTTTAGAAGCAGG+TGG 0.635598 2:-75258289 MsaT010586.1:CDS
AAGGGAGGGAGAGAAGTAGA+CGG 0.635894 2:+75256729 None:intergenic
GAATGATGAGGTTTACACAC+AGG 0.638216 2:-75258745 MsaT010586.1:CDS
TGCAAAAGACCTGCATGGTG+TGG 0.639765 2:-75257871 MsaT010586.1:CDS
AAGACCTGCATGGTGTGGAG+TGG 0.642527 2:-75257866 MsaT010586.1:CDS
ACATACACCATTGATTGTGG+AGG 0.643124 2:-75254984 MsaT010586.1:CDS
ATGTCTTGCAGGTGTAGTAG+CGG 0.648254 2:-75257078 MsaT010586.1:intron
CTTCTAAGAGATCCTGACAA+AGG 0.649192 2:-75255585 MsaT010586.1:CDS
GAACTAGAAGATGAACATGT+TGG 0.654160 2:+75259111 None:intergenic
AAGGCTGAAGAAACTGCGGA+CGG 0.656866 2:-75256688 MsaT010586.1:CDS
TGCTAAAAGAAGTTGCACGA+AGG 0.662737 2:-75255808 MsaT010586.1:intron
AAATTTCGCCATATTTATCG+AGG 0.663380 2:-75257843 MsaT010586.1:intron
GTAGACGGATCGATCTCCCA+AGG 0.663808 2:+75256744 None:intergenic
GGCCAATAAGGAGAATGATG+AGG 0.666485 2:-75258757 MsaT010586.1:CDS
ATACCAGTGATAAGGTGACT+GGG 0.667650 2:+75256648 None:intergenic
GTCGAAGGCTGAAGAAACTG+CGG 0.672621 2:-75256692 MsaT010586.1:CDS
CAATCTTCAGCAGCTCGACG+AGG 0.685556 2:+75258169 None:intergenic
TCTGATACCAGCACTCATGG+AGG 0.685802 2:-75258202 MsaT010586.1:CDS
TGAGTCTATAAGATCGACCA+AGG 0.694503 2:-75256519 MsaT010586.1:CDS
CTTGTTGCAAAAGACCTGCA+TGG 0.711702 2:-75257876 MsaT010586.1:CDS
AGAATCTGGCTGTCCCACTG+AGG 0.716343 2:+75254949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 75254925 75259244 75254925 ID=MsaG010586
Chr2 mRNA 75254925 75259244 75254925 ID=MsaT010586.1;Parent=MsaG010586
Chr2 exon 75254925 75255108 75254925 ID=MsaT010586.1.exon12;Parent=MsaT010586.1
Chr2 CDS 75254925 75255108 75254925 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75255522 75255712 75255522 ID=MsaT010586.1.exon11;Parent=MsaT010586.1
Chr2 CDS 75255522 75255712 75255522 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75255809 75256569 75255809 ID=MsaT010586.1.exon10;Parent=MsaT010586.1
Chr2 CDS 75255809 75256569 75255809 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75256652 75256814 75256652 ID=MsaT010586.1.exon9;Parent=MsaT010586.1
Chr2 CDS 75256652 75256814 75256652 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75257011 75257089 75257011 ID=MsaT010586.1.exon8;Parent=MsaT010586.1
Chr2 CDS 75257011 75257089 75257011 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75257167 75257286 75257167 ID=MsaT010586.1.exon7;Parent=MsaT010586.1
Chr2 CDS 75257167 75257286 75257167 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75257408 75257554 75257408 ID=MsaT010586.1.exon6;Parent=MsaT010586.1
Chr2 CDS 75257408 75257554 75257408 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75257667 75257757 75257667 ID=MsaT010586.1.exon5;Parent=MsaT010586.1
Chr2 CDS 75257667 75257757 75257667 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75257844 75257928 75257844 ID=MsaT010586.1.exon4;Parent=MsaT010586.1
Chr2 CDS 75257844 75257928 75257844 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75258156 75258341 75258156 ID=MsaT010586.1.exon3;Parent=MsaT010586.1
Chr2 CDS 75258156 75258341 75258156 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75258722 75258778 75258722 ID=MsaT010586.1.exon2;Parent=MsaT010586.1
Chr2 CDS 75258722 75258778 75258722 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Chr2 exon 75258897 75259244 75258897 ID=MsaT010586.1.exon1;Parent=MsaT010586.1
Chr2 CDS 75258897 75259244 75258897 ID=cds.MsaT010586.1;Parent=MsaT010586.1
Gene Sequence

>MsaT010586.1

ATGGATATTGATCTGAATGATTCCACAACACAAGTTAAGAAGAATATATGTTGCAATGGTGAATGTGAGAAAAGTGTTTGTTGTGTTTGCACTTTATCTTCTTCTTCATCACCAACATGTTCATCTTCTAGTTCCACTTCGGCTCTTGTTTCTTCATCTTATCTTGAACTATGGCATTCTTGTGCTGGACCTTTAACATCACTTCCAAAGAAAGGGAATGTTGTAGTTTATTTTCCACAAGGTCATTTGGAACAAGTTGCATCTTTGTCTCTCTTTTCTTCATTGGAAATTCCAACTTATGGTCTTCAACCACAAATCTTTTGTAGGGTTGTCAATGTTCAATTACTTGCCAATAAGGAGAATGATGAGGTTTACACACAGGTTACTTTGCTTCCACAGGCAGAGTTAGCAGGGATGTGTTTGGATGACAAAGAGCCTGAAGGTTTAGAAGCAGGTGGAGAGGGAGATGGAAGATCGCCTACAAAGTTAGCTTCTCACATGTTTTGCAAAACACTCACTGCCTCTGATACCAGCACTCATGGAGGGTTTTCTGTTCCTCGTCGAGCTGCTGAAGATTGTTTTCCTCCTTTGGATTATAAGCAGCAGAGACCCTCTCAAGAGCTTGTTGCAAAAGACCTGCATGGTGTGGAGTGGAAATTTCGCCATATTTATCGAGGTCAGCCAAGGAGGCATCTGCTCACTACTGGCTGGAGTGCTTTTGTTAGCCAAAAGAATCTTGTTTCTGGGGACGCAGTGCTCTTTCTAAGGGGTGAAAACGGAGAGTTAAGATTGGGAATCAGAAGAGCTGCTCGACCGAGAAACGGTCTTCCTGAATCAATTGTTGGTAACCAGAGTTGTTCTCCTAGTTTCCTTTCTTCAGTGGCTAATGCTATCTCCATAAAAGCATGTTTCATAGCTAGTCATGCCGATTTCGTTGTGCCTTACCAAAAGTATGCTAAAAGCATCAGGAACCCACTGACCATAGGGACAAGATTCAAAATGAAATTTGAAATGGATGAATCTCCAGAAAGAAGGTGTAGTAGCGGTATAGTGACTGGAATGAGTGATTTGGATCCCTATAAATGGCCCAAGTCAAAATGGAGATGCTTGATGGTCCGATGGGATGAGGATATTGAGGCTAATCATCAAGATAGAGTCTCTCCTTGGGAGATCGATCCGTCTACTTCTCTCCCTCCCTTGAACATTCAATCTTGTCGAAGGCTGAAGAAACTGCGGACGGGTCTGCAAGTTGAATCACCCAGTCACCTTATCACTGCTGGAGACAGTGGGTTTATGGACTTTGATGAGTCTATAAGATCGACCAAGGTCTTGCAAGGTCAAGAAAAAACAAGTTTTATGTCACTATATTATGGATGTGACACAGTAACTAAGCAGAAAGAATTTGATATCAATTCTCTAAGACATACTAATCTTGCATCAAATGGAGCAAGAAAGATTACTTCTTCCGAGTTTACGAGGGTTCAGCCTTCTAGTTATGCAGACTTCACGGAAACGAACCGGTTTCCTAGGGTCTTGCAAGGTCAAGAAATATATCCATTGAGATCCCTGGCAGGAAAGGTTGATCTAAACCTTAACTCTTGGGGTAAAACCAATGTTAGTTACACAAATTATAACCTGCATAATGCAACCAAACTTAACTTTCACTCATTAGGATCAGAAGTTCTTCAAAATTCCTCTTTTCCATATGGTGATATACACAAAGTTGGTCAAGGTAGCAGCATGCTGTGCTCCAAACCAACCAATTTCCAACTAGAGAATGTTTCATTTAATACTCCTTCAGCTCAGATAGGTGCATTGAGAAATGAAGTTGGAATATCAGGTTTCACAATCCGAAACAAGCAGAAGCTGCAGGACAATATTTCTGCTGCTGCTTCTCTAGGTGCAAACTTGAGGATTTCAAATGACAAAAATTTCAACGCGAAGGTAAATCCTTGTAGACTATTTGGATTTTCCTTATCGGCGGCGGAAACCACCTCACAAAATCTACAGAACTCTGCTAAAAGAAGTTGCACGAAGGTTCACAAACAAGGGAGCTTAGTTGGAAGAGCCATTGATCTCTCAAGATTGAGCAGCTACAATGATCTGGTGAGTGAACTAGAGAGACTATTTGGCATGGAAGGTCTTCTAAGAGATCCTGACAAAGGATGGAAAATCCTCTACACTGACAGTGAAAATGACATTATGGTTGTTGGGGATGATCCATGGCATGAGTTCTGTGTTATGGTATCAAAAATCCATATATATACACAAGAAGAAGTGGAGAAGATGACAATTGGGATGATGAATGATGATAACCAGAGTTGTTTGGAACATACACCATTGATTGTGGAGGCTTCAAAGTCTTCCTCAGTGGGACAGCCAGATTCTTCTCCAACAGTAGTTAGAATCTAG
Protein sequence

>MsaT010586.1

MDIDLNDSTTQVKKNICCNGECEKSVCCVCTLSSSSSPTCSSSSSTSALVSSSYLELWHSCAGPLTSLPKKGNVVVYFPQGHLEQVASLSLFSSLEIPTYGLQPQIFCRVVNVQLLANKENDEVYTQVTLLPQAELAGMCLDDKEPEGLEAGGEGDGRSPTKLASHMFCKTLTASDTSTHGGFSVPRRAAEDCFPPLDYKQQRPSQELVAKDLHGVEWKFRHIYRGQPRRHLLTTGWSAFVSQKNLVSGDAVLFLRGENGELRLGIRRAARPRNGLPESIVGNQSCSPSFLSSVANAISIKACFIASHADFVVPYQKYAKSIRNPLTIGTRFKMKFEMDESPERRCSSGIVTGMSDLDPYKWPKSKWRCLMVRWDEDIEANHQDRVSPWEIDPSTSLPPLNIQSCRRLKKLRTGLQVESPSHLITAGDSGFMDFDESIRSTKVLQGQEKTSFMSLYYGCDTVTKQKEFDINSLRHTNLASNGARKITSSEFTRVQPSSYADFTETNRFPRVLQGQEIYPLRSLAGKVDLNLNSWGKTNVSYTNYNLHNATKLNFHSLGSEVLQNSSFPYGDIHKVGQGSSMLCSKPTNFQLENVSFNTPSAQIGALRNEVGISGFTIRNKQKLQDNISAAASLGANLRISNDKNFNAKVNPCRLFGFSLSAAETTSQNLQNSAKRSCTKVHKQGSLVGRAIDLSRLSSYNDLVSELERLFGMEGLLRDPDKGWKILYTDSENDIMVVGDDPWHEFCVMVSKIHIYTQEEVEKMTIGMMNDDNQSCLEHTPLIVEASKSSSVGQPDSSPTVVRI*