AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010626

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010626 MtrunA17_Chr2g0326761 97.854 699 8 1 1 692 1 699 0.0 1416
MsaG010626 MtrunA17_Chr4g0028971 70.139 720 165 17 1 692 1 698 0.0 926
MsaG010626 MtrunA17_Chr1g0181211 57.593 698 228 16 2 659 18 687 0.0 751
MsaG010626 MtrunA17_Chr1g0199681 58.259 563 201 8 2 533 85 644 0.0 606
MsaG010626 MtrunA17_Chr7g0264171 53.394 545 216 10 1 518 21 554 0.0 556
MsaG010626 MtrunA17_Chr3g0115271 44.134 537 268 12 7 524 15 538 3.82e-125 384
MsaG010626 MtrunA17_Chr7g0264161 54.464 336 111 4 20 348 1 301 1.24e-121 364
MsaG010626 MtrunA17_Chr5g0424931 40.871 482 232 14 11 475 19 464 3.88e-100 317
MsaG010626 MtrunA17_Chr5g0417281 37.425 497 231 15 1 471 1 443 6.72e-89 287
MsaG010626 MtrunA17_Chr5g0436761 38.913 460 215 12 6 452 7 413 2.74e-87 281
MsaG010626 MtrunA17_Chr4g0070181 40.201 398 197 9 6 397 18 380 8.29e-85 289
MsaG010626 MtrunA17_Chr5g0433301 41.604 399 188 9 5 394 18 380 7.14e-84 282
MsaG010626 MtrunA17_Chr4g0046371 41.885 382 169 9 4 368 31 376 1.42e-83 278
MsaG010626 MtrunA17_Chr2g0282961 39.651 401 187 9 11 391 53 418 2.28e-83 275
MsaG010626 MtrunA17_Chr2g0285461 40.494 405 197 9 2 397 13 382 1.11e-82 280
MsaG010626 MtrunA17_Chr8g0385791 40.561 392 194 7 2 387 50 408 9.63e-82 276
MsaG010626 MtrunA17_Chr3g0109391 40.852 399 191 9 5 394 20 382 1.92e-81 276
MsaG010626 MtrunA17_Chr8g0376771 39.851 404 200 9 2 397 13 381 5.05e-81 276
MsaG010626 MtrunA17_Chr2g0301251 40.701 371 183 7 6 370 18 357 1.30e-80 278
MsaG010626 MtrunA17_Chr2g0277461 38.308 402 191 12 1 392 3 357 1.53e-80 263
MsaG010626 MtrunA17_Chr2g0289221 40.730 356 145 9 8 348 17 321 2.33e-80 259
MsaG010626 MtrunA17_Chr1g0178011 35.797 433 224 11 6 427 7 396 6.22e-80 262
MsaG010626 MtrunA17_Chr7g0239061 29.817 711 390 21 5 662 8 662 3.71e-79 266
MsaG010626 MtrunA17_Chr1g0156321 41.398 372 176 8 6 368 38 376 5.91e-78 266
MsaG010626 MtrunA17_Chr4g0029671 41.067 375 172 8 11 368 54 396 6.71e-78 265
MsaG010626 MtrunA17_Chr2g0326281 40.385 416 187 12 11 397 56 439 1.60e-77 265
MsaG010626 MtrunA17_Chr4g0009751 40.816 392 184 11 9 389 17 371 3.92e-77 259
MsaG010626 MtrunA17_Chr5g0417211 40.822 365 153 10 8 358 9 324 2.59e-76 251
MsaG010626 MtrunA17_Chr8g0389081 41.129 372 180 6 8 371 29 369 9.85e-76 264
MsaG010626 MtrunA17_Chr2g0276781 41.379 377 172 9 7 371 19 358 1.61e-75 256
MsaG010626 MtrunA17_Chr8g0346711 32.249 676 359 21 11 655 29 636 2.16e-75 256
MsaG010626 MtrunA17_Chr2g0277531 35.127 353 166 11 8 348 17 318 3.03e-61 207
MsaG010626 MtrunA17_Chr5g0424801 36.022 372 184 12 8 369 9 336 8.43e-58 200
MsaG010626 MtrunA17_Chr5g0425261 35.657 373 184 12 8 369 9 336 9.93e-58 200
MsaG010626 MtrunA17_Chr5g0424741 33.498 406 187 14 6 369 7 371 2.45e-55 197
MsaG010626 MtrunA17_Chr5g0425661 40.291 206 102 2 11 204 37 233 5.45e-49 172
MsaG010626 MtrunA17_Chr8g0356211 31.769 277 154 4 116 391 2 244 4.98e-37 140
MsaG010626 MtrunA17_Chr5g0425651 41.228 114 60 3 263 372 2 112 1.30e-19 85.1
MsaG010626 MtrunA17_Chr5g0424791 33.108 148 87 4 59 204 14 151 1.66e-19 85.9
MsaG010626 MtrunA17_Chr8g0345771 38.614 101 60 2 103 203 167 265 3.98e-12 69.3
MsaG010626 MtrunA17_Chr1g0178071 35.659 129 79 3 84 208 51 179 5.58e-11 64.7
MsaG010626 MtrunA17_Chr1g0198661 30.822 146 86 3 85 216 170 314 7.85e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010626 AT2G28350.1 58.985 729 223 19 3 692 2 693 0.0 769
MsaG010626 AT4G30080.1 57.664 685 232 15 5 662 13 666 0.0 739
MsaG010626 AT2G28350.2 58.890 703 213 19 3 666 2 667 0.0 737
MsaG010626 AT1G77850.1 38.761 565 296 16 5 542 13 554 2.52e-113 353
MsaG010626 AT1G77850.2 38.761 565 296 16 5 542 13 554 2.81e-113 354
MsaG010626 AT5G37020.2 41.470 381 184 6 3 375 14 363 2.29e-85 285
MsaG010626 AT5G37020.1 41.470 381 184 6 3 375 14 363 4.94e-85 285
MsaG010626 AT1G59750.1 33.632 669 351 20 11 655 22 621 1.30e-83 278
MsaG010626 AT1G59750.3 33.632 669 351 20 11 655 22 621 1.30e-83 278
MsaG010626 AT1G59750.2 33.533 668 350 20 11 655 22 618 2.37e-83 277
MsaG010626 AT1G59750.4 33.533 668 348 21 11 655 22 616 1.14e-82 275
MsaG010626 AT5G60450.2 40.796 402 187 8 2 387 32 398 5.32e-82 276
MsaG010626 AT1G19220.1 39.152 401 203 9 5 399 17 382 2.63e-81 280
MsaG010626 AT5G60450.1 41.221 393 180 8 11 387 65 422 2.64e-81 275
MsaG010626 AT2G33860.1 38.806 402 197 10 11 398 54 420 1.61e-80 268
MsaG010626 AT1G30330.1 40.594 404 196 9 3 397 14 382 3.81e-80 274
MsaG010626 AT1G30330.3 40.150 401 197 8 5 397 19 384 3.91e-80 274
MsaG010626 AT1G30330.2 40.150 401 197 8 5 397 19 384 3.91e-80 274
MsaG010626 AT5G62000.4 39.900 401 186 9 4 387 48 410 1.24e-79 271
MsaG010626 AT5G62000.2 39.900 401 186 9 4 387 48 410 1.39e-79 271
MsaG010626 AT5G62000.5 39.900 401 186 9 4 387 48 410 1.39e-79 271
MsaG010626 AT5G62000.3 39.900 401 186 9 4 387 48 410 1.39e-79 271
MsaG010626 AT5G62000.1 39.900 401 186 9 4 387 48 410 1.39e-79 271
MsaG010626 AT5G20730.2 38.750 400 204 9 6 399 19 383 6.94e-79 273
MsaG010626 AT4G23980.1 31.587 668 356 19 11 655 12 601 1.46e-78 263
MsaG010626 AT5G20730.1 38.653 401 204 10 6 399 19 384 1.40e-77 270
MsaG010626 AT5G20730.3 38.653 401 204 10 6 399 19 384 1.71e-77 270
MsaG010626 AT1G19850.1 40.270 370 180 8 8 368 51 388 9.59e-76 261
MsaG010626 AT1G19850.2 40.270 370 180 8 8 368 40 377 1.13e-75 261
MsaG010626 AT4G23980.2 40.431 371 176 9 11 372 12 346 2.61e-73 249
MsaG010626 AT2G46530.1 39.637 386 168 12 11 383 21 354 3.72e-72 245
MsaG010626 AT2G46530.3 39.119 386 170 11 11 383 42 375 5.84e-72 245
MsaG010626 AT3G61830.1 38.798 366 180 9 11 368 25 354 1.54e-68 236
MsaG010626 AT1G34410.1 29.198 661 354 19 11 655 27 589 6.57e-67 231
MsaG010626 AT1G34310.1 28.896 661 356 19 11 655 27 589 1.60e-64 224
MsaG010626 AT1G35240.1 29.242 660 341 21 11 655 27 575 2.51e-63 221
MsaG010626 AT1G34390.1 28.293 668 363 21 11 662 27 594 8.46e-63 220
MsaG010626 AT1G35520.1 29.085 667 347 21 11 655 27 589 5.59e-62 218
MsaG010626 AT5G37020.3 43.478 276 123 5 102 375 24 268 4.22e-60 215
MsaG010626 AT1G35540.1 36.458 384 180 11 11 382 27 358 7.78e-58 206
MsaG010626 AT2G33860.2 40.476 294 140 6 108 398 2 263 1.21e-57 202
MsaG010626 AT3G61830.2 39.062 320 157 8 51 368 20 303 2.24e-56 201
MsaG010626 AT1G34170.1 34.908 381 192 13 11 382 25 358 4.25e-55 197
MsaG010626 AT2G46530.2 38.849 278 123 7 107 383 36 267 1.71e-48 179
MsaG010626 AT1G34170.2 33.071 381 173 13 11 382 25 332 4.86e-48 176
MsaG010626 AT1G34170.3 33.508 382 170 14 11 382 25 332 4.77e-47 175
MsaG010626 AT1G43950.1 41.176 204 105 5 11 207 27 222 3.62e-40 147
MsaG010626 ATMG00940.1 38.854 157 79 5 195 343 1 148 8.69e-21 90.5
MsaG010626 AT1G13260.1 36.508 126 72 4 84 205 166 287 1.55e-12 70.1
MsaG010626 AT2G36080.3 34.043 141 85 4 103 238 33 170 1.79e-12 68.2
MsaG010626 AT2G36080.1 34.043 141 85 4 103 238 33 170 1.97e-12 68.2
MsaG010626 AT2G36080.4 37.838 111 63 3 103 208 33 142 2.28e-12 66.2
MsaG010626 AT2G36080.2 37.838 111 63 3 103 208 33 142 2.28e-12 66.2
MsaG010626 AT3G25730.1 36.275 102 61 2 108 205 183 284 1.35e-11 67.0
MsaG010626 AT1G01030.2 35.455 110 65 2 103 207 51 159 1.80e-11 66.6
MsaG010626 AT2G46870.1 35.965 114 67 3 100 208 27 139 2.03e-11 66.2
MsaG010626 AT1G01030.1 35.455 110 65 2 103 207 51 159 2.72e-11 66.2
MsaG010626 AT4G01500.1 38.462 91 51 2 108 197 36 122 3.11e-11 65.9

Find 145 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATCGTCTCTCGATTATT+TGG 0.140943 2:+75673865 None:intergenic
GAAAATACTTCATCAGTTTC+AGG 0.152637 2:+75675810 None:intergenic
GCACAATCCAACGTTGATTT+TGG 0.154632 2:-75675897 MsaT010626.1:CDS
CGCTCCTGCCGCAGGCATTC+AGG 0.178149 2:-75673965 MsaT010626.1:CDS
ATACAACACAAAGGGTTAAA+AGG 0.216528 2:+75674009 None:intergenic
TCGAAACCGAAGATTCTTCT+AGG 0.244012 2:-75675083 MsaT010626.1:CDS
GGGAACTATTTCTTCTGTTC+AGG 0.264499 2:-75675046 MsaT010626.1:CDS
TTCAAGCTCTGAATTTCTTA+AGG 0.295501 2:+75675770 None:intergenic
TTGACAATTCTGATGGATTC+AGG 0.309644 2:-75673175 MsaT010626.1:CDS
TTCCCCTTGGAGACTTCTTC+AGG 0.314727 2:-75674998 MsaT010626.1:intron
TTCACATTGTGTAGTAAATC+TGG 0.325303 2:+75674186 None:intergenic
AGAAGTCTCCAAGGGGAATT+AGG 0.326359 2:+75675003 None:intergenic
TTGAGAGAGGAGAATAAGTT+GGG 0.327890 2:-75675291 MsaT010626.1:CDS
AATTGTGGTCTTGGACCTTA+TGG 0.329009 2:-75675333 MsaT010626.1:CDS
AGAAAACTGGCTAAGATGTT+TGG 0.333734 2:-75673418 MsaT010626.1:CDS
ATGAGAATTCAATGGTGTTC+TGG 0.334279 2:-75675126 MsaT010626.1:CDS
TGAAGAACTTTATAGAAAAC+TGG 0.335874 2:-75673431 MsaT010626.1:CDS
ACACCTTCTGGATGGAGTTC+TGG 0.337274 2:-75675363 MsaT010626.1:CDS
TTTGAGAGAGGAGAATAAGT+TGG 0.338801 2:-75675292 MsaT010626.1:CDS
GCGAAGAGAGGGATTGTTAA+TGG 0.339338 2:-75675393 MsaT010626.1:CDS
GGAGCCAGGCATGCTCAAAT+TGG 0.343033 2:-75673943 MsaT010626.1:CDS
TCAAGCTCTGAATTTCTTAA+GGG 0.365482 2:+75675771 None:intergenic
TTCTAGGATTAGTTGGTTCA+TGG 0.372148 2:-75675067 MsaT010626.1:CDS
AATGGATTGGAAACACCTTC+TGG 0.376276 2:-75675375 MsaT010626.1:CDS
TCAAATTGGAATATCTTTAT+CGG 0.377207 2:-75673929 MsaT010626.1:CDS
TTTCTTTGCTCTTATTATCA+TGG 0.379754 2:+75673818 None:intergenic
ACTGAAATTTGTTGTTGTTA+AGG 0.379784 2:+75673899 None:intergenic
AAAATGTTGGTATTTGAAAC+TGG 0.386775 2:+75674040 None:intergenic
TTTGTGTTGTATATCTTCTT+CGG 0.389379 2:-75673998 MsaT010626.1:CDS
AAATTCTCACAAGAGAAGTT+TGG 0.389679 2:-75673770 MsaT010626.1:CDS
GTACTCTATCGCGATGCAAC+AGG 0.393430 2:-75673367 MsaT010626.1:CDS
AGGATGTTCACGGTGAGGTT+TGG 0.394252 2:-75675566 MsaT010626.1:CDS
AAGATTCTTCTAGGATTAGT+TGG 0.415313 2:-75675074 MsaT010626.1:CDS
TGCTAGTAATCAAACATTTG+AGG 0.416661 2:-75675214 MsaT010626.1:CDS
GCTCCTGCCGCAGGCATTCA+GGG 0.419621 2:-75673964 MsaT010626.1:CDS
AGGAGGCATTTGTTAACAAC+TGG 0.420761 2:-75675513 MsaT010626.1:CDS
AAGTCCATGGTTGGTCGAAT+TGG 0.421393 2:-75674157 MsaT010626.1:CDS
GCTCCCTGAATGCCTGCGGC+AGG 0.423621 2:+75673961 None:intergenic
ATATCATGTCTGTTGACTAT+AGG 0.423697 2:-75673793 MsaT010626.1:CDS
ATTAGATACTGGTCATTGTA+AGG 0.424326 2:-75673512 MsaT010626.1:CDS
ACGGTGAGGTTTGGAAGTTT+AGG 0.424396 2:-75675557 MsaT010626.1:CDS
GGTAATGGAAATTGTGGTCT+TGG 0.431909 2:-75675342 MsaT010626.1:CDS
ATTTGCAGGGTTTGTATCAC+AGG 0.434573 2:-75673045 MsaT010626.1:intron
GAGAGGGATTGTTAATGGAT+TGG 0.434835 2:-75675388 MsaT010626.1:CDS
TGGCAACTTGGATTAGATAC+TGG 0.435835 2:-75673523 MsaT010626.1:CDS
AACAACAACAAATTTCAGTT+AGG 0.435844 2:-75673895 MsaT010626.1:CDS
TTCAATGGTGTTCTGGGATG+AGG 0.437710 2:-75675119 MsaT010626.1:CDS
ACCAACACAAGACAAGTCTA+AGG 0.439710 2:+75673459 None:intergenic
AAAATACTTCATCAGTTTCA+GGG 0.442229 2:+75675811 None:intergenic
TGAGAATTCAATGGTGTTCT+GGG 0.446818 2:-75675125 MsaT010626.1:CDS
TTCTTCCTTGGTGGTGAGAA+GGG 0.447097 2:+75674105 None:intergenic
TTTCTTCCTTGGTGGTGAGA+AGG 0.447588 2:+75674104 None:intergenic
CCGCAGGCATTCAGGGAGCC+AGG 0.455283 2:-75673957 MsaT010626.1:CDS
TTCGTTGGCCTAATTCCCCT+TGG 0.456414 2:-75675011 MsaT010626.1:CDS
AAAGCTTAGATCCACAACTA+TGG 0.458962 2:-75676001 MsaT010626.1:CDS
ACCTTAGACTTGTCTTGTGT+TGG 0.459246 2:-75673460 MsaT010626.1:CDS
CAACTATGGCACGCATGTGC+TGG 0.459997 2:-75675987 MsaT010626.1:CDS
TGTGTTGTTGTCCAAGAAAA+AGG 0.460383 2:-75673647 MsaT010626.1:CDS
TAACACAACTGCAGGTCACT+TGG 0.460425 2:-75674215 MsaT010626.1:intron
ATTTGTTGCTCAGTGAGAAT+TGG 0.462091 2:+75673694 None:intergenic
TTCGCAATCACCGTCTGAAC+CGG 0.462939 2:+75675588 None:intergenic
TTTGCTCTTATTATCATGGT+TGG 0.466889 2:+75673822 None:intergenic
TACAACACAAAGGGTTAAAA+GGG 0.478847 2:+75674010 None:intergenic
GTTGTTAACAAATGCCTCCT+TGG 0.484302 2:+75675516 None:intergenic
TATTCCAATTTGAGCATGCC+TGG 0.486937 2:+75673939 None:intergenic
CAGGCAGCAAAGATACTAGA+AGG 0.487275 2:-75673156 MsaT010626.1:intron
TCTAGGATTAGTTGGTTCAT+GGG 0.488794 2:-75675066 MsaT010626.1:CDS
ACAGGTGCTGTTAAACAAAC+TGG 0.490968 2:-75673349 MsaT010626.1:CDS
AATTATAACGCTCCTGCCGC+AGG 0.493589 2:-75673973 MsaT010626.1:CDS
GGGGAATTAGGCCAACGAAT+AGG 0.495789 2:+75675015 None:intergenic
ACTAATCCTAGAAGAATCTT+CGG 0.501263 2:+75675077 None:intergenic
AAAGTCTTTGCAAAAGATGC+AGG 0.501554 2:+75675705 None:intergenic
AAGTTTAGGCATATCTATAG+AGG 0.501773 2:-75675543 MsaT010626.1:CDS
AATCCGGATGTTGTGGAAAA+CGG 0.502333 2:+75674079 None:intergenic
ACAACATCGAGAGGGAAATC+CGG 0.506711 2:+75674063 None:intergenic
AAACGGAGTTTCTTCCTTGG+TGG 0.510040 2:+75674096 None:intergenic
TGATTACTCGGCTGAGCCAC+CGG 0.514373 2:-75675607 MsaT010626.1:CDS
TCTTAAACAAAGATGCTCAT+AGG 0.516080 2:+75672976 None:intergenic
GCATGTTCTGCATGCCCTTG+TGG 0.516128 2:+75675918 None:intergenic
GGCATTTGTTAACAACTGGT+TGG 0.516900 2:-75675509 MsaT010626.1:CDS
GGAAAACGGAGTTTCTTCCT+TGG 0.523736 2:+75674093 None:intergenic
ATCGTTTCGGCACAGTATCT+AGG 0.523934 2:+75675642 None:intergenic
TCTGGATGGAGTTCTGGTAA+TGG 0.531132 2:-75675357 MsaT010626.1:CDS
AAGAAGATATACAACACAAA+GGG 0.535342 2:+75674001 None:intergenic
AGAGGGAAATCCGGATGTTG+TGG 0.540838 2:+75674072 None:intergenic
AAACTGGTGAAGAACCCTTC+AGG 0.541832 2:-75673333 MsaT010626.1:intron
GTATTCGTCGCGCGAAGAGA+GGG 0.550772 2:-75675404 MsaT010626.1:CDS
AGGTTGTTGATCCTATTCGT+TGG 0.555272 2:-75675026 MsaT010626.1:CDS
GGCTGAGCCACCGGTTCAGA+CGG 0.555712 2:-75675598 MsaT010626.1:CDS
TTAGATGCATCAAATAAGAC+AGG 0.558939 2:-75673000 MsaT010626.1:CDS
AAAGGGTTGCCTGAAAATGT+TGG 0.562489 2:+75674027 None:intergenic
GCACATGCGTGCCATAGTTG+TGG 0.562709 2:+75675990 None:intergenic
TCTGATGCAAACAATGGTGG+AGG 0.563598 2:-75675675 MsaT010626.1:CDS
GATACCAATTCGACCAACCA+TGG 0.567114 2:+75674153 None:intergenic
AACACAACTGCAGGTCACTT+GGG 0.568086 2:-75674214 MsaT010626.1:intron
CTATGGCACGCATGTGCTGG+AGG 0.570767 2:-75675984 MsaT010626.1:CDS
GGGTTTGTATCACAGGAACT+AGG 0.573351 2:-75673038 MsaT010626.1:CDS
GTGTTTCTGAGAGGTGAAAG+TGG 0.574136 2:-75675441 MsaT010626.1:CDS
GATTGGAAACACCTTCTGGA+TGG 0.576113 2:-75675371 MsaT010626.1:CDS
TGAAACTGGACAACATCGAG+AGG 0.579890 2:+75674054 None:intergenic
GCAATCACCGTCTGAACCGG+TGG 0.583092 2:+75675591 None:intergenic
ACCACTCATCCTCTGTAGAG+TGG 0.584023 2:-75675856 MsaT010626.1:CDS
TGAGAGAGGAGAATAAGTTG+GGG 0.584787 2:-75675290 MsaT010626.1:CDS
GAAGAATCTTCGGTTTCGAA+TGG 0.590530 2:+75675087 None:intergenic
GAAGTCTTAATACAAAACTC+AGG 0.592108 2:+75675165 None:intergenic
AGAATCTCCAAAATCAACGT+TGG 0.592692 2:+75675890 None:intergenic
GTGATTGCGAAGGATGTTCA+CGG 0.594396 2:-75675576 MsaT010626.1:CDS
GAGAGAGGAGAATAAGTTGG+GGG 0.597200 2:-75675289 MsaT010626.1:CDS
TGTGGTCTTGGACCTTATGG+TGG 0.601918 2:-75675330 MsaT010626.1:CDS
AAAGTCAGCACTAGCACTAG+TGG 0.602500 2:+75673549 None:intergenic
AACTCAGGAGTACTTGCTCT+AGG 0.603509 2:+75675180 None:intergenic
GGTATTCGTCGCGCGAAGAG+AGG 0.605126 2:-75675405 MsaT010626.1:CDS
TGCGAAGGATGTTCACGGTG+AGG 0.610344 2:-75675571 MsaT010626.1:CDS
GCACGCATGTGCTGGAGGGA+TGG 0.614096 2:-75675979 MsaT010626.1:CDS
TTCTTGGAGTCAGAGGATGT+TGG 0.618317 2:-75673487 MsaT010626.1:CDS
ATGTGAAGCGAGTAAGTCCA+TGG 0.621045 2:-75674170 MsaT010626.1:CDS
CAATCTGATGCAAACAATGG+TGG 0.622207 2:-75675678 MsaT010626.1:CDS
GCCACTCTACAGAGGATGAG+TGG 0.626388 2:+75675855 None:intergenic
TGTACCTGAAGAAGTCTCCA+AGG 0.627522 2:+75674994 None:intergenic
GAAGCGAGTAAGTCCATGGT+TGG 0.628522 2:-75674166 MsaT010626.1:CDS
CTTGAAAATGATGATTCTGA+TGG 0.628837 2:-75675753 MsaT010626.1:CDS
GGTTCAGACGGTGATTGCGA+AGG 0.635341 2:-75675586 MsaT010626.1:CDS
GTACCTGAAGAAGTCTCCAA+GGG 0.636379 2:+75674995 None:intergenic
AGTGAGTGGTGAATCAGTGA+AGG 0.641115 2:-75675253 MsaT010626.1:CDS
GAAGAAGATATACAACACAA+AGG 0.641708 2:+75674000 None:intergenic
CCTGGCTCCCTGAATGCCTG+CGG 0.642327 2:+75673957 None:intergenic
AGAGAGGAGAATAAGTTGGG+GGG 0.643897 2:-75675288 MsaT010626.1:CDS
CTGATGCAAACAATGGTGGA+GGG 0.644135 2:-75675674 MsaT010626.1:CDS
AGTTCTGGTAATGGAAATTG+TGG 0.651495 2:-75675348 MsaT010626.1:CDS
AATGATGATTCTGATGGTGA+TGG 0.651505 2:-75675747 MsaT010626.1:CDS
GGTGGAAGAGTGAAAGTGAG+TGG 0.652214 2:-75675267 MsaT010626.1:CDS
TTACCAGAACTCCATCCAGA+AGG 0.662357 2:+75675360 None:intergenic
GATTCTATTGTGTTTCTGAG+AGG 0.663178 2:-75675450 MsaT010626.1:CDS
GGAACTAGGAATGCTGAACG+TGG 0.666679 2:-75673024 MsaT010626.1:CDS
TATGGCACGCATGTGCTGGA+GGG 0.669654 2:-75675983 MsaT010626.1:CDS
GAAACTGGACAACATCGAGA+GGG 0.678914 2:+75674055 None:intergenic
TCACAGAAGCCACTCTACAG+AGG 0.682462 2:+75675847 None:intergenic
ACTCTACAGAGGATGAGTGG+TGG 0.690773 2:+75675858 None:intergenic
AAGCTGCTATGAGAATTCAA+TGG 0.696903 2:-75675134 MsaT010626.1:CDS
ACACAATCTGATGCAAACAA+TGG 0.698582 2:-75675681 MsaT010626.1:CDS
TCAGCCGAGTAATCAAGCCG+AGG 0.714702 2:+75675615 None:intergenic
TCGCACCCTTCTCACCACCA+AGG 0.717374 2:-75674110 MsaT010626.1:CDS
TCTATAGAGGAACACCAAGG+AGG 0.745721 2:-75675530 MsaT010626.1:CDS
TACCTGAAGAAGTCTCCAAG+GGG 0.745887 2:+75674996 None:intergenic
ATATCTATAGAGGAACACCA+AGG 0.792807 2:-75675533 MsaT010626.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 75672978 75676039 75672978 ID=MsaG010626
Chr2 mRNA 75672978 75676039 75672978 ID=MsaT010626.1;Parent=MsaG010626
Chr2 exon 75672978 75673059 75672978 ID=MsaT010626.1.exon4;Parent=MsaT010626.1
Chr2 CDS 75672978 75673059 75672978 ID=cds.MsaT010626.1;Parent=MsaT010626.1
Chr2 exon 75673157 75673222 75673157 ID=MsaT010626.1.exon3;Parent=MsaT010626.1
Chr2 CDS 75673157 75673222 75673157 ID=cds.MsaT010626.1;Parent=MsaT010626.1
Chr2 exon 75673334 75674223 75673334 ID=MsaT010626.1.exon2;Parent=MsaT010626.1
Chr2 CDS 75673334 75674223 75673334 ID=cds.MsaT010626.1;Parent=MsaT010626.1
Chr2 exon 75674999 75676039 75674999 ID=MsaT010626.1.exon1;Parent=MsaT010626.1
Chr2 CDS 75674999 75676039 75674999 ID=cds.MsaT010626.1;Parent=MsaT010626.1
Gene Sequence

>MsaT010626.1

ATGAAAGAAGCTGAGAAAAGCTTAGATCCACAACTATGGCACGCATGTGCTGGAGGGATGGTTCAAATGCCATCAGTGAACACAAAAGTGTTTTACTTTCCACAAGGGCATGCAGAACATGCACAATCCAACGTTGATTTTGGAGATTCTTTCAGAATTCCACCACTCATCCTCTGTAGAGTGGCTTCTGTGAAATTCTTAGCTGACCCTGAAACTGATGAAGTATTTTCAAAGATAACTTTGATTCCCTTAAGAAATTCAGAGCTTGAAAATGATGATTCTGATGGTGATGGTTCAGAGAATTCAGAAAAGCCTGCATCTTTTGCAAAGACTTTGACACAATCTGATGCAAACAATGGTGGAGGGTTCTCTGTTCCTAGATACTGTGCCGAAACGATTTTTCCTCGGCTTGATTACTCGGCTGAGCCACCGGTTCAGACGGTGATTGCGAAGGATGTTCACGGTGAGGTTTGGAAGTTTAGGCATATCTATAGAGGAACACCAAGGAGGCATTTGTTAACAACTGGTTGGAGTAGTTTTGTGAATCAGAAAAAGCTTGTTGCAGGTGATTCTATTGTGTTTCTGAGAGGTGAAAGTGGAGAACTTTTTGTTGGTATTCGTCGCGCGAAGAGAGGGATTGTTAATGGATTGGAAACACCTTCTGGATGGAGTTCTGGTAATGGAAATTGTGGTCTTGGACCTTATGGTGGTGCTTTTTCTGCTTTTTTGAGAGAGGAGAATAAGTTGGGGGGTGGAAGAGTGAAAGTGAGTGGTGAATCAGTGAAGGAAGCTATGAGACTTGCTGCTAGTAATCAAACATTTGAGGTTGTTTATTATCCTAGAGCAAGTACTCCTGAGTTTTGTATTAAGACTTCAGCTGTGAAAGCTGCTATGAGAATTCAATGGTGTTCTGGGATGAGGTTTAAGATGCCATTCGAAACCGAAGATTCTTCTAGGATTAGTTGGTTCATGGGAACTATTTCTTCTGTTCAGGTTGTTGATCCTATTCGTTGGCCTAATTCCCCTTGGAGACTTCTTCAGGTCACTTGGGACGAACCAGATTTACTACACAATGTGAAGCGAGTAAGTCCATGGTTGGTCGAATTGGTATCAAACATGTCGATGATTCATCTCGCACCCTTCTCACCACCAAGGAAGAAACTCCGTTTTCCACAACATCCGGATTTCCCTCTCGATGTTGTCCAGTTTCAAATACCAACATTTTCAGGCAACCCTTTTAACCCTTTGTGTTGTATATCTTCTTCGGATAATTATAACGCTCCTGCCGCAGGCATTCAGGGAGCCAGGCATGCTCAAATTGGAATATCTTTATCGGATCTTCACCTTAACAACAACAAATTTCAGTTAGGTGTGTTTCCAAATAATCGAGAGACGATTTCTAATGTTAGCAACATAACTACCAACCATGATAATAAGAGCAAAGAAAGTATATCATGTCTGTTGACTATAGGAAATTCTCACAAGAGAAGTTTGGAGATTAAATCTGATAATAATAATAAGAGACACCAATTTTTGCTTTTTGGTCAACCAATTCTCACTGAGCAACAAATCTCTAGAAAATTTTCTAGTGATGATGTGTTGTTGTCCAAGAAAAAGGACAAAGAGAAGTGGTTTTTCAGTGATACTACTCAATCTTCTATTTCTGAACAGTTTTCACCAGCAAAATCTTCTACCACTAGTGCTAGTGCTGACTTTTGTTGGCAACTTGGATTAGATACTGGTCATTGTAAGGTTTTCTTGGAGTCAGAGGATGTTGGAAGAACCTTAGACTTGTCTTGTGTTGGTTCTTATGAAGAACTTTATAGAAAACTGGCTAAGATGTTTGGAATAGAAAGATCTGAGATGTTGAGTCGTGTACTCTATCGCGATGCAACAGGTGCTGTTAAACAAACTGGTGAAGAACCCTTCAGTGACTTCATGAAGACAGCAAAAAGATTGACAATTCTGATGGATTCAGGCAGCAAAGATACTAGAAGGGTTTGTATCACAGGAACTAGGAATGCTGAACGTGGATTAGATGCATCAAATAAGACAGGACCTATGAGCATCTTTGTTTAA
Protein sequence

>MsaT010626.1

MKEAEKSLDPQLWHACAGGMVQMPSVNTKVFYFPQGHAEHAQSNVDFGDSFRIPPLILCRVASVKFLADPETDEVFSKITLIPLRNSELENDDSDGDGSENSEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSAEPPVQTVIAKDVHGEVWKFRHIYRGTPRRHLLTTGWSSFVNQKKLVAGDSIVFLRGESGELFVGIRRAKRGIVNGLETPSGWSSGNGNCGLGPYGGAFSAFLREENKLGGGRVKVSGESVKEAMRLAASNQTFEVVYYPRASTPEFCIKTSAVKAAMRIQWCSGMRFKMPFETEDSSRISWFMGTISSVQVVDPIRWPNSPWRLLQVTWDEPDLLHNVKRVSPWLVELVSNMSMIHLAPFSPPRKKLRFPQHPDFPLDVVQFQIPTFSGNPFNPLCCISSSDNYNAPAAGIQGARHAQIGISLSDLHLNNNKFQLGVFPNNRETISNVSNITTNHDNKSKESISCLLTIGNSHKRSLEIKSDNNNKRHQFLLFGQPILTEQQISRKFSSDDVLLSKKKDKEKWFFSDTTQSSISEQFSPAKSSTTSASADFCWQLGLDTGHCKVFLESEDVGRTLDLSCVGSYEELYRKLAKMFGIERSEMLSRVLYRDATGAVKQTGEEPFSDFMKTAKRLTILMDSGSKDTRRVCITGTRNAERGLDASNKTGPMSIFV*