AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010627

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010627 MtrunA17_Chr2g0326771 95.208 313 14 1 1 313 1 312 0.0 607
MsaG010627 MtrunA17_Chr4g0028961 53.459 318 125 7 8 302 1 318 1.08e-92 278
MsaG010627 MtrunA17_Chr1g0199671 47.312 186 70 6 124 281 121 306 5.05e-42 148
MsaG010627 MtrunA17_Chr1g0169431 50.000 142 47 3 140 281 119 236 4.81e-35 127
MsaG010627 MtrunA17_Chr1g0213161 72.603 73 20 0 215 287 150 222 5.63e-34 124
MsaG010627 MtrunA17_Chr7g0253101 66.667 84 28 0 202 285 173 256 1.11e-33 124
MsaG010627 MtrunA17_Chr6g0461591 77.143 70 16 0 217 286 192 261 3.88e-33 122
MsaG010627 MtrunA17_Chr5g0395391 70.130 77 23 0 205 281 258 334 7.11e-33 124
MsaG010627 MtrunA17_Chr5g0395391 65.789 76 26 0 205 280 156 231 1.27e-29 115
MsaG010627 MtrunA17_Chr8g0372291 48.936 141 62 4 142 281 151 282 1.28e-31 119
MsaG010627 MtrunA17_Chr1g0146231 76.923 65 15 0 217 281 234 298 1.72e-31 120
MsaG010627 MtrunA17_Chr4g0035891 75.385 65 16 0 217 281 211 275 3.09e-31 118
MsaG010627 MtrunA17_Chr3g0145121 67.123 73 24 0 209 281 207 279 3.21e-31 119
MsaG010627 MtrunA17_Chr8g0340401 75.385 65 16 0 217 281 226 290 1.64e-29 115
MsaG010627 MtrunA17_Chr5g0404981 73.846 65 17 0 217 281 300 364 2.96e-29 115
MsaG010627 MtrunA17_Chr3g0140331 75.385 65 16 0 217 281 253 317 4.33e-29 114
MsaG010627 MtrunA17_Chr7g0232701 40.496 121 69 1 158 278 17 134 9.29e-23 92.0
MsaG010627 MtrunA17_Chr7g0232681 52.055 73 35 0 206 278 121 193 9.63e-20 85.5
MsaG010627 MtrunA17_Chr7g0232671 56.061 66 29 0 217 282 187 252 3.96e-19 85.1
MsaG010627 MtrunA17_Chr7g0271861 57.778 45 19 0 208 252 13 57 1.40e-12 63.9
MsaG010627 MtrunA17_Chr5g0404131 55.263 38 17 0 212 249 187 224 7.47e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010627 AT1G08010.2 40.767 287 138 11 15 281 10 284 1.38e-54 180
MsaG010627 AT1G08010.1 40.767 287 138 11 15 281 10 284 1.38e-54 180
MsaG010627 AT1G08010.3 40.767 287 138 11 15 281 10 284 1.38e-54 180
MsaG010627 AT1G08000.2 40.441 272 145 8 21 281 17 282 4.15e-52 174
MsaG010627 AT1G08000.1 40.441 272 145 8 21 281 17 282 4.15e-52 174
MsaG010627 AT2G28340.1 35.943 281 138 10 12 281 6 255 6.95e-40 141
MsaG010627 AT3G54810.1 70.238 84 25 0 208 291 220 303 1.71e-34 128
MsaG010627 AT3G54810.2 70.238 84 25 0 208 291 220 303 1.71e-34 128
MsaG010627 AT4G32890.1 49.640 139 52 4 146 281 138 261 2.92e-33 124
MsaG010627 AT3G24050.1 64.286 84 27 1 201 281 175 258 4.42e-33 123
MsaG010627 AT4G36240.1 42.759 145 68 3 147 291 109 238 1.11e-31 119
MsaG010627 AT5G25830.1 47.368 152 59 5 147 281 136 283 3.24e-31 119
MsaG010627 AT3G60530.1 68.354 79 22 1 206 281 144 222 3.89e-31 117
MsaG010627 AT2G45050.1 64.103 78 28 0 204 281 166 243 2.23e-30 115
MsaG010627 AT5G66320.2 59.574 94 27 2 192 281 227 313 1.46e-29 115
MsaG010627 AT5G66320.1 59.574 94 27 2 192 281 227 313 1.46e-29 115
MsaG010627 AT4G34680.1 62.500 80 28 1 204 281 165 244 2.84e-29 113
MsaG010627 AT4G34680.2 62.500 80 28 1 204 281 165 244 2.84e-29 113
MsaG010627 AT3G51080.1 72.308 65 18 0 217 281 221 285 4.56e-28 110
MsaG010627 AT3G45170.1 51.111 90 44 0 192 281 90 179 7.74e-27 105
MsaG010627 AT5G26930.1 54.762 42 19 0 211 252 20 61 3.89e-11 60.1
MsaG010627 AT4G26150.1 32.710 107 60 2 143 247 133 229 4.99e-11 63.2
MsaG010627 AT2G18380.1 40.278 72 34 2 187 249 53 124 6.56e-11 61.2

Find 68 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTTCGGGCACGCTTTAC+TGG 0.142278 2:+75728601 None:intergenic
CTGTTCGCCGGCCGGTATTC+AGG 0.236111 2:+75728280 None:intergenic
GCTTCCTCAGTTGAGAATTC+TGG 0.240866 2:-75728643 MsaT010627.1:CDS
TTCTTTCATTGCTAGCTTAC+AGG 0.246670 2:-75728539 MsaT010627.1:CDS
ACACTGTCCTACAGAATTTC+CGG 0.284166 2:+75728141 None:intergenic
ACAAGCATTGCAGAGCGTTT+TGG 0.323869 2:+75728333 None:intergenic
AGAACTGCTGGACCAATATA+TGG 0.343881 2:-75728754 MsaT010627.1:CDS
AGAGAAGCCTTCCCCGAGTT+GGG 0.363900 2:+75732142 None:intergenic
TACTTCGGGCACGCTTTACT+GGG 0.367389 2:+75728602 None:intergenic
AGGACGACGGCGTTTACTTC+GGG 0.370626 2:+75728588 None:intergenic
AACCAAGATCCCTTCATATT+CGG 0.371276 2:+75732350 None:intergenic
TAGTCATCAAAGACGGTGTT+AGG 0.392164 2:-75728195 MsaT010627.1:CDS
TTCCGAATATGAAGGGATCT+TGG 0.400481 2:-75732352 MsaT010627.1:CDS
GAAGAAGATTGGAGTGCTCT+TGG 0.409178 2:-75732233 MsaT010627.1:CDS
TAGAAAGGGGCATTCTGCTT+GGG 0.445514 2:+75728721 None:intergenic
CAAACACTGCTGAAGAAGAT+TGG 0.445593 2:-75732244 MsaT010627.1:CDS
AAAGAAGCGGCAAGAACTGC+TGG 0.447119 2:-75728766 MsaT010627.1:CDS
AAACAGTCATAAGAAGGTTC+TGG 0.454626 2:-75728230 MsaT010627.1:CDS
CACTGTCCTACAGAATTTCC+GGG 0.456379 2:+75728142 None:intergenic
TTGGCTTCATCAAATGTTCC+CGG 0.465571 2:-75728160 MsaT010627.1:CDS
ACAAAAGTGGGGCTGTTCGC+CGG 0.466878 2:+75728268 None:intergenic
TCTGTAGGACAGTGTTATCC+TGG 0.467939 2:-75728133 MsaT010627.1:CDS
AATACAGGATTTAAGCTTGA+AGG 0.479268 2:+75728568 None:intergenic
ATGAAAGAAATCGAGAATAC+AGG 0.480881 2:+75728553 None:intergenic
ATGCTTGTGGTGTTCGGTAC+AGG 0.486984 2:-75728318 MsaT010627.1:CDS
ATAGAAAGGGGCATTCTGCT+TGG 0.489652 2:+75728720 None:intergenic
ACATTTGATGAAGCCAACTT+AGG 0.491700 2:+75728166 None:intergenic
AAGGACGACGGCGTTTACTT+CGG 0.496252 2:+75728587 None:intergenic
TCTGCAATGCTTGTGGTGTT+CGG 0.497409 2:-75728324 MsaT010627.1:CDS
CAGAGAAGCCTTCCCCGAGT+TGG 0.499799 2:+75732141 None:intergenic
TGTGGTGTTCGGTACAGGTC+TGG 0.506214 2:-75728313 MsaT010627.1:CDS
TGCACAGATGCTACAAAAGT+GGG 0.515410 2:+75728256 None:intergenic
ACAGGATATTCCGAATATGA+AGG 0.520393 2:-75732360 MsaT010627.1:intron
AAAATGAGAATCCCCAACTC+GGG 0.526899 2:-75732154 MsaT010627.1:CDS
GCACGCTTTACTGGGATGAC+AGG 0.530384 2:+75728610 None:intergenic
AAGTGGGGCTGTTCGCCGGC+CGG 0.532753 2:+75728272 None:intergenic
TGTTAGGATCCGTCCTAAGT+TGG 0.534235 2:-75728179 MsaT010627.1:CDS
CCGGCCGGTATTCAGGATAG+AGG 0.538514 2:+75728287 None:intergenic
TTCCCCAATAATAAAAGAAG+CGG 0.551958 2:-75728779 MsaT010627.1:CDS
GTGCACAGATGCTACAAAAG+TGG 0.558433 2:+75728255 None:intergenic
TTGGGTCCCTCTCTCCATTG+TGG 0.572384 2:+75728361 None:intergenic
GAGAAGCCTTCCCCGAGTTG+GGG 0.575311 2:+75732143 None:intergenic
TTCTTCAACAACTATGCCTG+AGG 0.576937 2:-75733248 None:intergenic
GAGAATCCCCAACTCGGGGA+AGG 0.582084 2:-75732149 MsaT010627.1:CDS
GCACTCAAACAGTCATAAGA+AGG 0.584644 2:-75728236 MsaT010627.1:CDS
TTGATGAAGCCAACTTAGGA+CGG 0.591529 2:+75728170 None:intergenic
TTTGCGTCCACATCTTCCAA+TGG 0.593963 2:+75732263 None:intergenic
GTCGCCTCTATCCTGAATAC+CGG 0.595004 2:-75728291 MsaT010627.1:CDS
AAAACGCTCTGCAATGCTTG+TGG 0.598857 2:-75728331 MsaT010627.1:CDS
GAGATCATCGAAAGTCTCAT+CGG 0.601725 2:+75732301 None:intergenic
AGTTGTGCTTCAATACAGCC+CGG 0.602280 2:-75728689 MsaT010627.1:CDS
CAGGATATTCCGAATATGAA+GGG 0.603930 2:-75732359 MsaT010627.1:CDS
AATGTTCCCGGAAATTCTGT+AGG 0.604813 2:-75728148 MsaT010627.1:CDS
GAGAAAGTGCACACATTGTG+AGG 0.609475 2:-75728395 MsaT010627.1:CDS
GAGACTCCACAATGGAGAGA+GGG 0.617903 2:-75728367 MsaT010627.1:CDS
AGAGACTCCACAATGGAGAG+AGG 0.626282 2:-75728368 MsaT010627.1:CDS
GTGTCCCCAGCAGTATTCTG+TGG 0.631226 2:-75732185 MsaT010627.1:CDS
GACAACTGACTCTTGTGATG+AGG 0.655145 2:+75728420 None:intergenic
CCTCTATCCTGAATACCGGC+CGG 0.661479 2:-75728287 MsaT010627.1:CDS
AAAGCCAGAATTCTCAACTG+AGG 0.662049 2:+75728639 None:intergenic
GATTTAAGCTTGAAGGACGA+CGG 0.671757 2:+75728575 None:intergenic
AAATGAGAATCCCCAACTCG+GGG 0.675438 2:-75732153 MsaT010627.1:CDS
TTTACCACAGAATACTGCTG+GGG 0.680114 2:+75732181 None:intergenic
AAGAAGGTTCTGGAGATGCG+AGG 0.685588 2:-75728220 MsaT010627.1:CDS
CGAGGCGTAGTCATCAAAGA+CGG 0.686995 2:-75728202 MsaT010627.1:CDS
CAATGGAGAGAGGGACCCAA+CGG 0.691262 2:-75728358 MsaT010627.1:CDS
GCACAGATGCTACAAAAGTG+GGG 0.711092 2:+75728257 None:intergenic
AGGTCACAGAGACTCCACAA+TGG 0.715521 2:-75728375 MsaT010627.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 75728123 75733257 75728123 ID=MsaG010627
Chr2 mRNA 75728123 75733257 75728123 ID=MsaT010627.1;Parent=MsaG010627
Chr2 exon 75728123 75728812 75728123 ID=MsaT010627.1.exon3;Parent=MsaT010627.1
Chr2 CDS 75728123 75728812 75728123 ID=cds.MsaT010627.1;Parent=MsaT010627.1
Chr2 exon 75732136 75732378 75732136 ID=MsaT010627.1.exon2;Parent=MsaT010627.1
Chr2 CDS 75732136 75732378 75732136 ID=cds.MsaT010627.1;Parent=MsaT010627.1
Chr2 exon 75733249 75733257 75733249 ID=MsaT010627.1.exon1;Parent=MsaT010627.1
Chr2 CDS 75733249 75733257 75733249 ID=cds.MsaT010627.1;Parent=MsaT010627.1
Gene Sequence

>MsaT010627.1

ATGCCTGAGGATATTCCGAATATGAAGGGATCTTGGTTTTTTGACAAAAATTTTAATGGTTTGTCCGATGAGACTTTCGATGATCTCAAGTTTTTCGATTTTCCATTGGAAGATGTGGACGCAAACACTGCTGAAGAAGATTGGAGTGCTCTTGGAGAGCCGTGTTTTGATGTTTTTTCAGTGTCCCCAGCAGTATTCTGTGGTAAAATTAAAAATGAGAATCCCCAACTCGGGGAAGGCTTCTCTGCTCCTTTTAATGACATTTCCCCAATAATAAAAGAAGCGGCAAGAACTGCTGGACCAATATATGGAAAAACTATTCCCAAGCAGAATGCCCCTTTCTATGAAAAAAAAGTTGTGCTTCAATACAGCCCGGTTTCTGTTTTTGAAGGCAGCAGTGCTTCCTCAGTTGAGAATTCTGGCTTTGATCTGCCTGTCATCCCAGTAAAGCGTGCCCGAAGTAAACGCCGTCGTCCTTCAAGCTTAAATCCTGTATTCTCGATTTCTTTCATTGCTAGCTTACAGGCTTTGCATAAGAAGACATCTGCTTTTGAGTCAGATATAAACAGAGTAAAGAAACAGAAAAAAAGGGAGCTCTCGGGTGATATTGAAACGAAGAAATCCTCATCACAAGAGTCAGTTGTCACGAGAAAGTGCACACATTGTGAGGTCACAGAGACTCCACAATGGAGAGAGGGACCCAACGGTCCCAAAACGCTCTGCAATGCTTGTGGTGTTCGGTACAGGTCTGGTCGCCTCTATCCTGAATACCGGCCGGCGAACAGCCCCACTTTTGTAGCATCTGTGCACTCAAACAGTCATAAGAAGGTTCTGGAGATGCGAGGCGTAGTCATCAAAGACGGTGTTAGGATCCGTCCTAAGTTGGCTTCATCAAATGTTCCCGGAAATTCTGTAGGACAGTGTTATCCTGGATCTTTTTGA
Protein sequence

>MsaT010627.1

MPEDIPNMKGSWFFDKNFNGLSDETFDDLKFFDFPLEDVDANTAEEDWSALGEPCFDVFSVSPAVFCGKIKNENPQLGEGFSAPFNDISPIIKEAARTAGPIYGKTIPKQNAPFYEKKVVLQYSPVSVFEGSSASSVENSGFDLPVIPVKRARSKRRRPSSLNPVFSISFIASLQALHKKTSAFESDINRVKKQKKRELSGDIETKKSSSQESVVTRKCTHCEVTETPQWREGPNGPKTLCNACGVRYRSGRLYPEYRPANSPTFVASVHSNSHKKVLEMRGVVIKDGVRIRPKLASSNVPGNSVGQCYPGSF*