AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010695

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010695 MtrunA17_Chr2g0328021 91.475 305 6 5 1 302 1 288 0.0 553
MsaG010695 MtrunA17_Chr4g0031411 43.099 355 119 17 1 296 1 331 6.65e-62 199
MsaG010695 MtrunA17_Chr4g0010151 40.549 328 108 17 1 261 1 308 1.22e-47 163
MsaG010695 MtrunA17_Chr7g0237921 37.818 275 115 6 1 238 1 256 2.59e-43 151
MsaG010695 MtrunA17_Chr7g0223311 52.500 160 45 5 1 146 1 143 6.47e-42 148
MsaG010695 MtrunA17_Chr8g0367161 56.000 100 41 1 55 154 12 108 4.33e-33 124
MsaG010695 MtrunA17_Chr8g0374251 62.069 87 29 1 78 164 68 150 1.70e-32 122
MsaG010695 MtrunA17_Chr3g0117601 52.475 101 48 0 28 128 11 111 2.25e-31 119
MsaG010695 MtrunA17_Chr4g0047001 68.919 74 18 1 79 147 56 129 2.64e-31 120
MsaG010695 MtrunA17_Chr8g0341671 76.119 67 14 1 73 139 15 79 7.10e-31 115
MsaG010695 MtrunA17_Chr2g0326001 64.935 77 27 0 56 132 18 94 1.11e-30 117
MsaG010695 MtrunA17_Chr4g0030051 76.923 65 14 1 67 130 29 93 1.34e-30 116
MsaG010695 MtrunA17_Chr5g0411951 82.143 56 10 0 73 128 38 93 1.78e-30 117
MsaG010695 MtrunA17_Chr3g0127201 71.642 67 19 0 67 133 16 82 1.88e-30 116
MsaG010695 MtrunA17_Chr3g0126201 70.149 67 20 0 68 134 41 107 5.70e-30 115
MsaG010695 MtrunA17_Chr8g0374111 52.000 100 44 2 71 166 22 121 9.18e-30 112
MsaG010695 MtrunA17_Chr4g0061761 86.538 52 7 0 77 128 39 90 1.12e-29 114
MsaG010695 MtrunA17_Chr2g0308011 90.000 50 5 0 77 126 36 85 1.44e-28 109
MsaG010695 MtrunA17_Chr8g0346551 72.581 62 17 0 75 136 21 82 2.76e-28 110
MsaG010695 MtrunA17_Chr2g0282511 75.362 69 17 0 77 145 44 112 3.22e-28 110
MsaG010695 MtrunA17_Chr2g0282441 84.314 51 8 0 79 129 26 76 1.55e-27 109
MsaG010695 MtrunA17_Chr1g0188851 68.333 60 19 0 75 134 40 99 6.28e-27 106
MsaG010695 MtrunA17_Chr2g0282051 50.538 93 27 2 42 134 28 101 8.75e-27 105
MsaG010695 MtrunA17_Chr1g0177251 77.358 53 12 0 80 132 14 66 1.00e-26 106
MsaG010695 MtrunA17_Chr4g0047761 75.862 58 13 1 78 135 72 128 1.83e-26 106
MsaG010695 MtrunA17_Chr5g0417571 44.118 102 57 0 30 131 90 191 5.68e-26 104
MsaG010695 MtrunA17_Chr6g0453321 52.273 88 41 1 44 131 103 189 5.91e-26 107
MsaG010695 MtrunA17_Chr2g0283811 56.410 78 30 1 64 141 37 110 7.69e-26 100
MsaG010695 MtrunA17_Chr8g0355261 51.163 86 42 0 56 141 96 181 1.61e-25 105
MsaG010695 MtrunA17_Chr6g0460721 58.824 68 28 0 65 132 76 143 3.37e-24 100
MsaG010695 MtrunA17_Chr7g0217581 61.538 65 25 0 67 131 221 285 6.33e-24 101
MsaG010695 MtrunA17_Chr7g0254161 59.701 67 27 0 65 131 88 154 8.96e-24 100
MsaG010695 MtrunA17_Chr3g0092111 57.534 73 30 1 59 131 118 189 9.51e-24 100
MsaG010695 MtrunA17_Chr5g0417601 47.778 90 47 0 42 131 104 193 1.26e-23 99.4
MsaG010695 MtrunA17_Chr6g0460731 56.716 67 29 0 65 131 83 149 1.98e-23 98.6
MsaG010695 MtrunA17_Chr4g0042481 41.481 135 72 3 75 207 119 248 2.32e-23 99.4
MsaG010695 MtrunA17_Chr5g0417551 47.126 87 45 1 45 131 95 180 1.30e-21 93.6
MsaG010695 MtrunA17_Chr5g0417561 53.333 75 32 1 57 131 101 172 5.25e-21 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010695 AT3G55370.1 39.498 319 157 14 1 290 1 312 1.08e-47 162
MsaG010695 AT3G55370.2 42.975 242 114 9 1 224 1 236 8.08e-46 158
MsaG010695 AT3G55370.3 37.742 310 158 13 9 290 55 357 5.11e-41 146
MsaG010695 AT2G37590.1 51.351 148 55 4 1 134 1 145 1.45e-40 144
MsaG010695 AT2G28810.1 48.408 157 41 4 1 129 1 145 1.03e-37 137
MsaG010695 AT5G65590.1 72.500 80 17 1 65 139 23 102 8.49e-35 128
MsaG010695 AT5G02460.1 56.303 119 35 2 39 157 73 174 1.28e-34 130
MsaG010695 AT2G28810.2 61.702 94 24 1 36 129 47 128 7.52e-34 126
MsaG010695 AT1G07640.1 73.611 72 18 1 64 135 8 78 2.24e-33 124
MsaG010695 AT5G60200.1 65.333 75 26 0 71 145 48 122 4.49e-33 122
MsaG010695 AT1G07640.2 73.611 72 18 1 64 135 64 134 5.12e-33 124
MsaG010695 AT1G07640.3 73.611 72 18 1 64 135 72 142 7.82e-33 124
MsaG010695 AT1G51700.1 47.934 121 63 0 53 173 8 128 1.93e-32 119
MsaG010695 AT5G60850.1 67.532 77 23 1 77 151 50 126 2.57e-32 122
MsaG010695 AT5G66940.1 56.322 87 38 0 64 150 18 104 1.06e-30 115
MsaG010695 AT3G45610.1 63.889 72 26 0 68 139 30 101 2.58e-30 115
MsaG010695 AT5G62940.1 78.333 60 13 0 69 128 64 123 1.13e-29 116
MsaG010695 AT2G28510.1 78.333 60 13 0 77 136 47 106 1.44e-29 114
MsaG010695 AT4G21050.1 57.647 85 33 1 64 148 13 94 1.54e-29 112
MsaG010695 AT3G21270.1 86.792 53 7 0 78 130 29 81 2.00e-29 111
MsaG010695 AT3G52440.1 63.158 76 28 0 74 149 21 96 2.79e-29 112
MsaG010695 AT3G52440.2 63.158 76 28 0 74 149 40 115 3.67e-29 112
MsaG010695 AT1G28310.1 77.586 58 13 0 78 135 27 84 5.02e-29 113
MsaG010695 AT4G38000.1 73.770 61 16 0 72 132 35 95 7.58e-29 111
MsaG010695 AT1G28310.2 77.586 58 13 0 78 135 51 108 7.96e-29 113
MsaG010695 AT1G21340.1 71.667 60 17 0 76 135 36 95 1.85e-28 110
MsaG010695 AT3G50410.1 74.576 59 15 0 78 136 30 88 8.41e-28 108
MsaG010695 AT4G24060.1 66.667 66 22 0 67 132 42 107 1.33e-27 110
MsaG010695 AT3G61850.1 41.667 144 76 4 22 165 20 155 5.75e-27 107
MsaG010695 AT3G61850.2 41.958 143 75 4 23 165 9 143 5.92e-27 107
MsaG010695 AT3G61850.3 41.958 143 75 4 23 165 9 143 5.92e-27 107
MsaG010695 AT3G61850.4 41.958 143 75 4 23 165 21 155 8.85e-27 107
MsaG010695 AT3G61850.5 53.398 103 38 4 63 165 51 143 8.94e-27 107
MsaG010695 AT1G47655.1 68.182 66 20 1 62 126 12 77 2.40e-26 103
MsaG010695 AT4G00940.1 77.358 53 12 0 80 132 70 122 2.79e-25 102
MsaG010695 AT4G00940.3 77.358 53 12 0 80 132 70 122 2.79e-25 102
MsaG010695 AT4G00940.2 77.358 53 12 0 80 132 70 122 2.79e-25 102
MsaG010695 AT1G64620.1 68.852 61 19 0 67 127 38 98 3.67e-25 103
MsaG010695 AT2G34140.1 70.175 57 17 0 75 131 55 111 7.70e-25 98.6
MsaG010695 AT1G29160.1 70.175 57 17 0 75 131 59 115 1.24e-24 98.2
MsaG010695 AT2G46590.1 78.431 51 11 0 75 125 65 115 2.19e-24 101
MsaG010695 AT2G46590.2 78.431 51 11 0 75 125 77 127 2.45e-24 101
MsaG010695 AT5G39660.3 58.108 74 30 1 58 131 119 191 5.84e-24 101
MsaG010695 AT5G39660.1 58.108 74 30 1 58 131 119 191 5.84e-24 101
MsaG010695 AT5G39660.2 58.108 74 30 1 58 131 119 191 5.84e-24 101
MsaG010695 AT3G47500.1 48.352 91 40 2 47 131 74 163 6.87e-24 101
MsaG010695 AT1G69570.1 63.492 63 23 0 75 137 129 191 2.11e-23 99.4
MsaG010695 AT1G26790.1 56.164 73 29 1 59 131 95 164 2.54e-23 99.0
MsaG010695 AT5G62430.1 47.126 87 43 1 45 131 24 107 3.48e-23 97.1
MsaG010695 AT4G21040.1 35.955 178 74 8 80 218 27 203 1.57e-22 94.0
MsaG010695 AT4G21080.1 61.905 63 20 1 80 142 27 85 1.11e-21 92.0
MsaG010695 AT4G21030.1 49.296 71 32 1 70 140 17 83 1.82e-15 73.9

Find 73 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGACATGTAGGCGTTAT+TGG 0.219753 2:+76731382 MsaT010695.1:CDS
TTGAACCAACAACTACTTCT+AGG 0.230645 2:+76731793 MsaT010695.1:CDS
TTCTCATTACTATTTGATAT+TGG 0.260955 2:-76731501 None:intergenic
CAGTTTCCTTTCATGAATAA+TGG 0.306796 2:+76731720 MsaT010695.1:CDS
TCAACAATTTCACTTTGAAA+TGG 0.326972 2:-76731774 None:intergenic
TGAAGAGATGTCATGTAATT+AGG 0.330958 2:-76731594 None:intergenic
TTATTCTCTGACATATTCAT+TGG 0.339957 2:-76731885 None:intergenic
CAACAATTTCACTTTGAAAT+GGG 0.353913 2:-76731773 None:intergenic
ACAACCATTACAATAATTCA+TGG 0.381419 2:+76731907 MsaT010695.1:CDS
GTTGGAAACATGTTTCAAAT+TGG 0.387187 2:+76731636 MsaT010695.1:CDS
GGAAGCAACTGATGTTGATT+TGG 0.388221 2:-76731123 None:intergenic
AGCTTGATCCGGTATCGAAC+CGG 0.392442 2:-76731193 None:intergenic
TCATGGTCTTGCATCTTCTT+CGG 0.406074 2:+76731938 MsaT010695.1:CDS
CAAGAGGAGTTGAAGTTGAA+TGG 0.407718 2:-76731545 None:intergenic
ATGGTGCAGGCTCAATTATC+AGG 0.408827 2:+76731169 MsaT010695.1:CDS
GATGCCTGCATCGGTTAAAT+CGG 0.412501 2:+76731827 MsaT010695.1:CDS
TGTAATTAGGTCTATTAGTT+GGG 0.420123 2:-76731581 None:intergenic
ATGTAATTAGGTCTATTAGT+TGG 0.434589 2:-76731582 None:intergenic
CTTATTTAGATCCTCTCAAT+TGG 0.441172 2:+76730895 MsaT010695.1:CDS
AAGGAGGAGTTGTGGATCAG+TGG 0.442180 2:+76731688 MsaT010695.1:CDS
CATTCAACTTCAACTCCTCT+TGG 0.446006 2:+76731546 MsaT010695.1:CDS
TGAAGTTCAAACTGGTCATC+AGG 0.450714 2:+76731233 MsaT010695.1:CDS
TATCAGGCCCGGTTCGATAC+CGG 0.455922 2:+76731185 MsaT010695.1:CDS
CTACGACACCCTCCACCAAC+AGG 0.460856 2:-76731432 None:intergenic
AAAAGTGTCTTGGTTGAGAC+AGG 0.461945 2:-76731359 None:intergenic
TTGAGAGGATCTAAATAAGA+TGG 0.471759 2:-76730891 None:intergenic
CAGTCCATGAATTATTGTAA+TGG 0.478494 2:-76731911 None:intergenic
TCAAGTACTGGTGGAGGAGA+AGG 0.484627 2:+76731669 MsaT010695.1:CDS
ACATGTTTCCAACACCGAAC+CGG 0.484940 2:-76731626 None:intergenic
GGTGATTATAGTTCAAGTAC+TGG 0.489772 2:+76731657 MsaT010695.1:CDS
TGAACCAACAACTACTTCTA+GGG 0.497338 2:+76731794 MsaT010695.1:CDS
GCCTGAGCTTGAGCTTGATC+CGG 0.498212 2:-76731204 None:intergenic
TGTTCCGATTTAACCGATGC+AGG 0.498710 2:-76731831 None:intergenic
GCAGCTCATGAAGTTCAAAC+TGG 0.502505 2:+76731225 MsaT010695.1:CDS
CTTCAGAATCTGAACCGGTT+CGG 0.507338 2:+76731612 MsaT010695.1:CDS
CATCTCTTCAGAATCTGAAC+CGG 0.507412 2:+76731607 MsaT010695.1:CDS
AATCTGAACCGGTTCGGTGT+TGG 0.507539 2:+76731618 MsaT010695.1:CDS
TACTACTTTGATGCCTGCAT+CGG 0.514344 2:+76731818 MsaT010695.1:CDS
GTAACCCTAGAAGTAGTTGT+TGG 0.518165 2:-76731798 None:intergenic
TTAAGAAATGTTCCTGTTGG+TGG 0.525025 2:+76731420 MsaT010695.1:CDS
ACCGGATCAAGCTCAAGCTC+AGG 0.531426 2:+76731203 MsaT010695.1:CDS
CATGTCTTGCAAAAGTGTCT+TGG 0.535988 2:-76731369 None:intergenic
TCATCACAGCCACATGGTGC+AGG 0.545230 2:+76731156 MsaT010695.1:CDS
TCAAAACCATTATTCATGAA+AGG 0.546534 2:-76731726 None:intergenic
CACTTCAAAGCTGTCTCTGA+AGG 0.551798 2:-76731285 None:intergenic
AGAAATGTTCCTGTTGGTGG+AGG 0.558097 2:+76731423 MsaT010695.1:CDS
CTTACTTGCTGCCAATTGAG+AGG 0.568314 2:-76730906 None:intergenic
AAATCGGAACACAACGGAGG+AGG 0.573191 2:+76731843 MsaT010695.1:CDS
GCATTAAGAAATGTTCCTGT+TGG 0.573758 2:+76731417 MsaT010695.1:CDS
GCTTGATCCGGTATCGAACC+GGG 0.575393 2:-76731192 None:intergenic
GCAGGCTCAATTATCAGGCC+CGG 0.575596 2:+76731174 MsaT010695.1:CDS
AAGGTGGTGGTAACTTAGCT+TGG 0.577015 2:-76731266 None:intergenic
AGTACTGGTGGAGGAGAAGG+AGG 0.578007 2:+76731672 MsaT010695.1:CDS
AAAGCTGTCTCTGAAGGTGG+TGG 0.586444 2:-76731279 None:intergenic
TATAGTTCAAGTACTGGTGG+AGG 0.593548 2:+76731663 MsaT010695.1:CDS
GATTATAGTTCAAGTACTGG+TGG 0.600312 2:+76731660 MsaT010695.1:CDS
GTAGGCGTTATTGGACAAGA+GGG 0.605161 2:+76731391 MsaT010695.1:CDS
TGTGGCTGTGATGAGGATAG+TGG 0.611946 2:-76731147 None:intergenic
TGGAGGAGAAGGAGGAGTTG+TGG 0.612099 2:+76731680 MsaT010695.1:CDS
TGTAGGCGTTATTGGACAAG+AGG 0.616737 2:+76731390 MsaT010695.1:CDS
TTCAAAGCTGTCTCTGAAGG+TGG 0.619079 2:-76731282 None:intergenic
GAAATGTTCCTGTTGGTGGA+GGG 0.644271 2:+76731424 MsaT010695.1:CDS
CTATCCTCATCACAGCCACA+TGG 0.646501 2:+76731150 MsaT010695.1:CDS
GGCTGTGATGAGGATAGTGG+TGG 0.646948 2:-76731144 None:intergenic
TGCACCATGTGGCTGTGATG+AGG 0.663808 2:-76731154 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 2:+76731392 MsaT010695.1:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 2:+76731393 MsaT010695.1:CDS
ATAATTGAGCCTGCACCATG+TGG 0.676504 2:-76731165 None:intergenic
TGGAATAGAAGAGAAAACCA+TGG 0.712223 2:-76730871 None:intergenic
GGGTGTAAGAATTCACCAAG+AGG 0.726422 2:-76731561 None:intergenic
GTGTTGGTTGATTCACAACG+AGG 0.729681 2:-76731309 None:intergenic
GTTAAATCGGAACACAACGG+AGG 0.733463 2:+76731840 MsaT010695.1:CDS
TCGGTTAAATCGGAACACAA+CGG 0.745806 2:+76731837 MsaT010695.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 76730873 76731977 76730873 ID=MsaG010695
Chr2 mRNA 76730873 76731977 76730873 ID=MsaT010695.1;Parent=MsaG010695
Chr2 exon 76730873 76730923 76730873 ID=MsaT010695.1.exon1;Parent=MsaT010695.1
Chr2 CDS 76730873 76730923 76730873 ID=cds.MsaT010695.1;Parent=MsaT010695.1
Chr2 exon 76731120 76731977 76731120 ID=MsaT010695.1.exon2;Parent=MsaT010695.1
Chr2 CDS 76731120 76731977 76731120 ID=cds.MsaT010695.1;Parent=MsaT010695.1
Gene Sequence

>MsaT010695.1

ATGGTTTTCTCTTCTATTCCATCTTATTTAGATCCTCTCAATTGGCAGCAACAACCAAATCAACATCAGTTGCTTCCACCACTATCCTCATCACAGCCACATGGTGCAGGCTCAATTATCAGGCCCGGTTCGATACCGGATCAAGCTCAAGCTCAGGCAGCTCATGAAGTTCAAACTGGTCATCAGGTTCAAACTCACCAAGCTAAGTTACCACCACCTTCAGAGACAGCTTTGAAGTGTCCTCGTTGTGAATCAACCAACACAAAATTTTGTTACTACAACAACTATAACCTGTCTCAACCAAGACACTTTTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCATTAAGAAATGTTCCTGTTGGTGGAGGGTGTCGTAGAAGCAAAAAGACCAAAAAGAGTAACACCTCTACTCCCTCAAAATCTCCAATATCAAATAGTAATGAGAAAGATTATTCAACAAGTGCAATCCATTCAACTTCAACTCCTCTTGGTGAATTCTTACACCCAACTAATAGACCTAATTACATGACATCTCTTCAGAATCTGAACCGGTTCGGTGTTGGAAACATGTTTCAAATTGGTGATTATAGTTCAAGTACTGGTGGAGGAGAAGGAGGAGTTGTGGATCAGTGGAGATTTCAGCAGTTTCCTTTCATGAATAATGGTTTTGATTCAAGTACTTCAAATGTTTCTTTCCCATTTCAAAGTGAAATTGTTGAACCAACAACTACTTCTAGGGTTACTACTTTGATGCCTGCATCGGTTAAATCGGAACACAACGGAGGAGGTTTTAATCTGCTTAGATCTCCAATGAATATGTCAGAGAATAACAACCATTACAATAATTCATGGACTGATTTTCATGGTCTTGCATCTTCTTCGGCTAGTCGTCTGTTATAA
Protein sequence

>MsaT010695.1

MVFSSIPSYLDPLNWQQQPNQHQLLPPLSSSQPHGAGSIIRPGSIPDQAQAQAAHEVQTGHQVQTHQAKLPPPSETALKCPRCESTNTKFCYYNNYNLSQPRHFCKTCRRYWTRGGALRNVPVGGGCRRSKKTKKSNTSTPSKSPISNSNEKDYSTSAIHSTSTPLGEFLHPTNRPNYMTSLQNLNRFGVGNMFQIGDYSSSTGGGEGGVVDQWRFQQFPFMNNGFDSSTSNVSFPFQSEIVEPTTTSRVTTLMPASVKSEHNGGGFNLLRSPMNMSENNNHYNNSWTDFHGLASSSASRLL*