AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010724

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010724 MtrunA17_Chr2g0328391 96.303 541 19 1 203 743 132 671 0.0 1085
MsaG010724 MtrunA17_Chr2g0328391 94.776 134 7 0 1 134 1 134 4.54e-83 277
MsaG010724 MtrunA17_Chr4g0031711 57.823 735 251 12 21 743 48 735 0.0 804
MsaG010724 MtrunA17_Chr4g0031701 56.897 696 206 10 54 743 21 628 0.0 764
MsaG010724 MtrunA17_Chr4g0031731 56.466 696 209 10 54 743 21 628 0.0 752
MsaG010724 MtrunA17_Chr4g0031721 48.892 722 313 17 35 743 8 686 0.0 625
MsaG010724 MtrunA17_Chr7g0238891 44.585 711 330 17 61 743 68 742 0.0 566
MsaG010724 MtrunA17_Chr4g0031741 52.677 467 192 6 294 743 198 652 4.38e-167 496
MsaG010724 MtrunA17_Chr2g0328431 50.855 468 209 5 284 743 202 656 1.42e-162 484
MsaG010724 MtrunA17_Chr2g0328411 51.893 449 206 4 295 741 214 654 2.17e-162 485
MsaG010724 MtrunA17_Chr2g0328341 52.516 457 200 8 291 743 197 640 2.53e-159 476
MsaG010724 MtrunA17_Chr2g0328381 51.230 447 200 9 302 743 210 643 2.10e-152 458
MsaG010724 MtrunA17_Chr2g0328401 49.889 449 201 5 284 721 200 635 8.28e-149 448
MsaG010724 MtrunA17_Chr2g0328361 49.103 446 186 10 302 743 209 617 4.62e-139 422
MsaG010724 MtrunA17_Chr2g0328371 56.641 256 105 3 443 697 2 252 2.06e-98 304
MsaG010724 MtrunA17_Chr2g0319091 32.979 376 245 4 367 740 208 578 5.41e-67 231
MsaG010724 MtrunA17_Chr3g0101141 32.021 381 242 8 367 740 171 541 9.49e-61 214
MsaG010724 MtrunA17_Chr4g0076091 30.607 379 255 5 366 741 170 543 1.13e-60 213
MsaG010724 MtrunA17_Chr5g0445331 33.505 388 231 10 367 740 157 531 4.26e-60 211
MsaG010724 MtrunA17_Chr5g0447691 30.851 376 253 4 367 740 173 543 9.35e-60 211
MsaG010724 MtrunA17_Chr7g0224271 31.472 394 235 6 366 740 295 672 3.07e-56 204
MsaG010724 MtrunA17_Chr1g0159461 29.679 374 256 4 369 740 223 591 9.35e-55 198
MsaG010724 MtrunA17_Chr2g0290241 28.571 378 262 5 366 740 52 424 1.68e-54 193
MsaG010724 MtrunA17_Chr3g0125811 30.851 376 249 7 369 740 201 569 2.09e-54 197
MsaG010724 MtrunA17_Chr7g0245601 31.793 368 235 6 375 742 442 793 2.18e-52 195
MsaG010724 MtrunA17_Chr8g0376381 31.937 382 237 8 362 740 160 521 1.80e-51 187
MsaG010724 MtrunA17_Chr2g0306641 31.316 380 245 7 365 740 119 486 6.84e-51 185
MsaG010724 MtrunA17_Chr3g0110971 31.218 394 231 6 366 740 214 586 1.44e-50 186
MsaG010724 MtrunA17_Chr8g0357601 32.648 389 240 8 366 743 177 554 2.74e-50 185
MsaG010724 MtrunA17_Chr4g0057891 29.950 404 246 10 356 740 134 519 3.18e-50 184
MsaG010724 MtrunA17_Chr4g0038761 28.382 377 242 6 375 743 88 444 1.75e-45 169
MsaG010724 MtrunA17_Chr3g0085681 26.555 418 250 6 372 740 26 435 2.84e-44 165
MsaG010724 MtrunA17_Chr4g0057291 29.199 387 252 10 367 742 288 663 2.21e-43 167
MsaG010724 MtrunA17_Chr4g0038851 26.402 428 250 5 371 740 48 468 2.55e-43 163
MsaG010724 MtrunA17_Chr1g0183641 27.586 406 263 12 343 740 94 476 3.57e-41 157
MsaG010724 MtrunA17_Chr2g0294461 28.354 395 256 8 362 740 201 584 1.11e-36 145
MsaG010724 MtrunA17_Chr4g0039661 26.509 381 255 10 368 740 191 554 1.09e-35 142
MsaG010724 MtrunA17_Chr5g0401131 28.125 384 258 7 371 742 114 491 3.28e-34 137
MsaG010724 MtrunA17_Chr5g0424131 26.253 419 271 12 346 741 96 499 5.30e-32 130
MsaG010724 MtrunA17_Chr2g0294471 26.020 392 266 7 362 740 195 575 9.59e-32 130
MsaG010724 MtrunA17_Chr1g0200401 27.456 397 243 14 371 740 80 458 1.18e-30 126
MsaG010724 MtrunA17_Chr3g0114841 26.024 415 259 13 356 741 105 500 1.41e-30 126
MsaG010724 MtrunA17_Chr7g0270031 28.093 388 248 11 366 740 184 553 2.13e-30 126
MsaG010724 MtrunA17_Chr6g0468561 26.963 382 250 12 372 740 296 661 9.68e-30 125
MsaG010724 MtrunA17_Chr7g0236851 25.867 375 259 8 372 740 295 656 1.75e-29 124
MsaG010724 MtrunA17_Chr3g0085361 25.140 358 210 7 432 740 1 349 1.21e-28 118
MsaG010724 MtrunA17_Chr5g0401421 21.934 424 276 8 368 743 32 448 5.15e-27 115
MsaG010724 MtrunA17_Chr4g0053901 22.803 421 274 9 368 743 56 470 6.95e-27 115
MsaG010724 MtrunA17_Chr5g0396491 29.048 210 142 2 371 580 56 258 4.90e-26 112
MsaG010724 MtrunA17_Chr5g0396491 28.188 149 107 0 592 740 329 477 1.62e-17 86.3
MsaG010724 MtrunA17_Chr1g0194621 25.505 396 255 13 366 740 141 517 7.52e-26 112
MsaG010724 MtrunA17_Chr2g0329591 26.733 303 204 6 371 667 74 364 7.71e-24 105
MsaG010724 MtrunA17_Chr8g0383981 24.121 398 263 16 359 743 127 498 1.38e-22 102
MsaG010724 MtrunA17_Chr7g0230731 23.280 378 258 8 372 740 373 727 5.47e-17 85.5
MsaG010724 MtrunA17_Chr8g0344101 24.365 394 242 14 358 727 215 576 6.92e-17 84.7
MsaG010724 MtrunA17_Chr7g0230771 23.016 378 259 8 372 740 376 730 7.95e-17 85.1
MsaG010724 MtrunA17_Chr4g0012071 24.458 323 215 8 376 691 201 501 1.81e-16 83.2
MsaG010724 MtrunA17_Chr2g0323111 25.000 232 162 6 522 743 79 308 1.53e-14 75.5
MsaG010724 MtrunA17_Chr8g0373561 22.691 379 262 12 372 740 183 540 6.75e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010724 AT1G07530.1 49.000 700 315 10 68 743 86 767 0.0 634
MsaG010724 AT2G29060.2 47.323 691 267 14 62 742 53 656 0.0 583
MsaG010724 AT2G29060.1 47.338 695 298 14 62 742 53 693 0.0 579
MsaG010724 AT1G07520.1 45.586 691 295 12 69 743 70 695 0.0 560
MsaG010724 AT1G07520.3 45.586 691 295 12 69 743 71 696 0.0 560
MsaG010724 AT2G29065.1 44.695 754 282 18 1 743 1 630 0.0 557
MsaG010724 AT1G07520.2 51.337 561 218 10 194 743 15 531 0.0 548
MsaG010724 AT1G07520.4 51.337 561 218 10 194 743 15 531 0.0 548
MsaG010724 AT2G37650.1 41.690 710 318 15 61 742 73 714 1.87e-171 510
MsaG010724 AT2G37650.2 41.690 710 318 15 61 742 61 702 3.48e-171 509
MsaG010724 AT3G46600.3 50.783 447 207 5 297 743 116 549 2.93e-158 470
MsaG010724 AT3G46600.1 50.783 447 207 5 297 743 148 581 3.58e-158 471
MsaG010724 AT5G59450.1 47.410 502 246 8 257 743 102 600 1.55e-157 470
MsaG010724 AT3G46600.2 55.053 376 166 2 368 743 1 373 1.74e-150 443
MsaG010724 AT1G50600.3 31.915 376 249 4 367 740 155 525 4.07e-60 212
MsaG010724 AT1G50600.1 31.915 376 249 4 367 740 226 596 1.53e-59 212
MsaG010724 AT1G50600.2 31.915 376 249 4 367 740 230 600 2.44e-59 211
MsaG010724 AT5G48150.1 28.967 397 276 3 344 740 99 489 1.58e-58 206
MsaG010724 AT5G48150.4 28.967 397 276 3 344 740 99 489 1.58e-58 206
MsaG010724 AT5G48150.2 28.967 397 276 3 344 740 99 489 1.58e-58 206
MsaG010724 AT5G48150.3 28.967 397 276 3 344 740 99 489 1.58e-58 206
MsaG010724 AT2G04890.1 31.016 374 248 4 367 740 49 412 2.83e-58 204
MsaG010724 AT1G21450.1 29.825 456 303 9 292 740 147 592 5.33e-58 207
MsaG010724 AT1G66350.1 33.245 379 226 9 366 740 150 505 8.96e-52 188
MsaG010724 AT3G54220.1 30.095 422 277 7 324 742 245 651 1.29e-51 191
MsaG010724 AT4G17230.1 31.842 380 250 5 366 742 153 526 3.43e-51 187
MsaG010724 AT4G17230.2 31.842 380 250 5 366 742 153 526 3.43e-51 187
MsaG010724 AT2G01570.1 31.217 378 241 8 366 740 219 580 5.75e-49 182
MsaG010724 AT3G03450.1 29.894 378 251 8 366 740 178 544 5.91e-49 181
MsaG010724 AT1G14920.1 31.746 378 239 8 366 740 167 528 1.62e-48 180
MsaG010724 AT5G66770.1 33.231 325 203 7 375 696 224 537 1.88e-47 177
MsaG010724 AT1G50420.1 26.047 430 250 8 371 740 57 478 4.25e-44 166
MsaG010724 AT5G17490.1 27.632 380 251 8 366 740 155 515 2.42e-42 162
MsaG010724 AT3G50650.1 30.294 340 212 7 381 711 185 508 1.74e-39 154
MsaG010724 AT1G63100.1 31.871 342 214 9 367 701 248 577 5.49e-39 154
MsaG010724 AT1G63100.2 31.871 342 214 9 367 701 273 602 5.49e-39 154
MsaG010724 AT5G41920.1 27.559 381 251 7 366 740 38 399 5.93e-39 149
MsaG010724 AT3G49950.1 28.900 391 248 12 371 742 30 409 4.63e-36 141
MsaG010724 AT5G52510.1 25.773 388 258 10 369 740 266 639 1.19e-35 144
MsaG010724 AT4G37650.1 26.463 393 262 9 370 742 145 530 1.09e-33 136
MsaG010724 AT1G55580.1 27.187 423 239 12 368 740 41 444 9.68e-31 126
MsaG010724 AT4G08250.1 24.769 432 289 17 326 740 64 476 1.08e-24 108
MsaG010724 AT3G13840.1 23.934 422 275 14 330 740 112 498 4.91e-18 88.6
MsaG010724 AT4G00150.1 23.704 405 257 13 351 740 186 553 2.28e-15 80.5
MsaG010724 AT5G67411.1 30.846 201 125 8 488 677 1 198 1.44e-11 64.7

Find 173 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCTTCACATTATTGATTT+TGG 0.112468 2:+77077440 MsaT010724.1:CDS
CATCAAAGACAAAATCTTTA+TGG 0.121774 2:-77078150 None:intergenic
CCAGTTTCTGGTGGTGATTC+TGG 0.150366 2:+77076465 MsaT010724.1:CDS
TCACCTGATACACCAGTTTC+TGG 0.198731 2:+77076453 MsaT010724.1:CDS
TTGAATGGTTTCCCAGTTTC+GGG 0.204080 2:-77077663 None:intergenic
TTTGTTCAATCCATAGTAAA+TGG 0.225738 2:+77077830 MsaT010724.1:CDS
TGTTGTGTTCCTACTGATTC+TGG 0.235374 2:+77076396 MsaT010724.1:CDS
ATCTTCAAAGGTAGAATCTT+TGG 0.257325 2:-77076178 None:intergenic
CCTCATTGCTCTTAATATTA+AGG 0.263212 2:-77077694 None:intergenic
TTCAAGTTCAAATTTGAATT+CGG 0.265372 2:+77076803 MsaT010724.1:CDS
TTTGAATGGTTTCCCAGTTT+CGG 0.285892 2:-77077664 None:intergenic
AGGCCATACCATCCCGAAAC+TGG 0.299357 2:+77077651 MsaT010724.1:CDS
CAGCAGCACTAAACATCTTT+TGG 0.300712 2:-77077313 None:intergenic
AGGAAGCATTCTTCTCCTTC+AGG 0.315645 2:+77077197 MsaT010724.1:CDS
TGCCGGTCGAAAGCCAGCTT+GGG 0.317241 2:-77077558 None:intergenic
CTCAAACTGAAACCATGATT+TGG 0.321027 2:-77076519 None:intergenic
TATCAAACATTGCAGAATAA+TGG 0.321158 2:-77077901 None:intergenic
ATTATAAGACCCATTTACTA+TGG 0.323109 2:-77077840 None:intergenic
AACTTCAGAACTGCATCTTT+AGG 0.332688 2:-77077782 None:intergenic
AAATCGTTAAGGTTTGGATT+TGG 0.332771 2:-77076090 None:intergenic
AGTGGCAGGTTAGGAATCTA+AGG 0.333559 2:+77078056 MsaT010724.1:CDS
AATCTTCTTCCATTAGAATC+TGG 0.335576 2:-77076233 None:intergenic
CCACCAGAAACTGGTGTATC+AGG 0.337940 2:-77076456 None:intergenic
TCAAGTTCAAATTTGAATTC+GGG 0.339297 2:+77076804 MsaT010724.1:CDS
TCAAGATGGAGGGTGGTGTT+AGG 0.341116 2:+77076760 MsaT010724.1:CDS
GGAATCTAAGGGCTGGCTTT+AGG 0.342541 2:+77078068 MsaT010724.1:CDS
GCAGTTTAGAAAAGGGTTAG+AGG 0.342843 2:+77076635 MsaT010724.1:CDS
TTCTTCTGAGACTAGGGTTA+TGG 0.353283 2:+77076578 MsaT010724.1:CDS
TGTGGATTTGAGGACCCTTT+TGG 0.355547 2:+77077109 MsaT010724.1:CDS
CAGTTTATTGTTCCAAATCA+TGG 0.358564 2:+77076507 MsaT010724.1:CDS
AAATATCTGATATGTTTGAT+AGG 0.361784 2:+77076952 MsaT010724.1:CDS
TACGTATGCTTCGGCTGGTT+GGG 0.371904 2:+77078213 MsaT010724.1:CDS
ATGAATTGTTGAGGCAGATT+AGG 0.382955 2:+77077177 MsaT010724.1:CDS
CAGCCGAAGCATACGTAATT+CGG 0.384019 2:-77078207 None:intergenic
ACATCAAGAGGGTGTTGATT+AGG 0.390520 2:-77076351 None:intergenic
GTTTCGAATCCGACAAAGTT+CGG 0.391177 2:-77076021 None:intergenic
CATACAAACAGTGGCAGGTT+AGG 0.391689 2:+77078047 MsaT010724.1:CDS
CTGCCGGTCGAAAGCCAGCT+TGG 0.397922 2:-77077559 None:intergenic
ATTAAGGTCTTCAATTTGAA+TGG 0.400958 2:-77077678 None:intergenic
TTACGTATGCTTCGGCTGGT+TGG 0.401437 2:+77078212 MsaT010724.1:CDS
CAAGATGGAGGGTGGTGTTA+GGG 0.405701 2:+77076761 MsaT010724.1:CDS
CAATGTTTCCTTCGAATACA+AGG 0.408276 2:+77077631 MsaT010724.1:CDS
ATGGTGAAATCGTTAAGGTT+TGG 0.408700 2:-77076096 None:intergenic
ATGCAGTTCTGAAGTTAATC+AGG 0.410047 2:+77077789 MsaT010724.1:CDS
GAATTGGATGGGCCCACATA+AGG 0.415523 2:-77076126 None:intergenic
CTGCCACTGTTTGTATGTCT+CGG 0.422026 2:-77078041 None:intergenic
CCAGAATCACCACCAGAAAC+TGG 0.422848 2:-77076465 None:intergenic
CTGTGTCTGCTAATGACAAT+AGG 0.426533 2:+77077147 MsaT010724.1:CDS
ATTTGGATTCTTCTGAGACT+AGG 0.434021 2:+77076571 MsaT010724.1:CDS
ATATATAAGCCAGATTCTAA+TGG 0.435212 2:+77076224 MsaT010724.1:CDS
AGTTCAAATTCGAATTCAAA+TGG 0.435366 2:+77076834 MsaT010724.1:CDS
TAATGGCCAGATTTAGAGAT+AGG 0.438554 2:+77078113 MsaT010724.1:CDS
TAGGCAACTCCCACTGGATA+AGG 0.439045 2:+77078087 MsaT010724.1:CDS
TTTGGATTCTTCTGAGACTA+GGG 0.441086 2:+77076572 MsaT010724.1:CDS
CACCATGACCAACCCTTATG+TGG 0.441330 2:+77076113 MsaT010724.1:CDS
ACTCAGGTTGGTGAGCTTGA+TGG 0.441788 2:+77077044 MsaT010724.1:CDS
TCTTAGCCTATCTCTAAATC+TGG 0.445660 2:-77078119 None:intergenic
TTCCCAGTTTCGGGATGGTA+TGG 0.447770 2:-77077654 None:intergenic
CGCATGGTTGGTGCTGGTAC+CGG 0.452661 2:+77077269 MsaT010724.1:CDS
TGGTCATGGTGAAATCGTTA+AGG 0.453510 2:-77076101 None:intergenic
AATTCCTGTGATCCTCAACT+TGG 0.460110 2:-77077528 None:intergenic
TTGTTCAATCCATAGTAAAT+GGG 0.464694 2:+77077831 MsaT010724.1:CDS
TCTTTGATGAAGATAACAAC+TGG 0.466798 2:+77078164 MsaT010724.1:CDS
TTTAGTGTCTCATGAGCCTA+AGG 0.471760 2:+77076722 MsaT010724.1:CDS
TGGCTTTAGGCAACTCCCAC+TGG 0.476205 2:+77078081 MsaT010724.1:CDS
ACCATGGTCATCGAATTTGT+AGG 0.476706 2:-77076046 None:intergenic
GCAGAGAGAATAGAGGAAAC+TGG 0.479525 2:+77077578 MsaT010724.1:CDS
GATAACAACTGGATGCTTCA+AGG 0.480041 2:+77078175 MsaT010724.1:CDS
TCATATCCCGTGAAAATGAA+TGG 0.481425 2:+77077936 MsaT010724.1:CDS
AGAAGGAAACTGTGGATTTG+AGG 0.481509 2:+77077099 MsaT010724.1:CDS
GCTATGGATCCGAACTTTGT+CGG 0.481965 2:+77076012 None:intergenic
TGCCACTGTTTGTATGTCTC+GGG 0.483709 2:-77078040 None:intergenic
TTATGTGGGCCCATCCAATT+CGG 0.491734 2:+77076128 MsaT010724.1:CDS
AATTGGATGGGCCCACATAA+GGG 0.496632 2:-77076125 None:intergenic
TGGCTGTGAACTGTTTGGTA+AGG 0.497180 2:+77077720 MsaT010724.1:CDS
CCCACTGGATAAGGAGGTAA+TGG 0.498395 2:+77078096 MsaT010724.1:CDS
CGAATTACGTATGCTTCGGC+TGG 0.503997 2:+77078208 MsaT010724.1:CDS
TTGAAGCGTGTGGAAAAGAA+AGG 0.504187 2:-77077866 None:intergenic
AATATCTGATATGTTTGATA+GGG 0.504258 2:+77076953 MsaT010724.1:CDS
GGAACACAACAATTGCTACT+AGG 0.506690 2:-77076384 None:intergenic
GGGTGTTGATTAGGTGAAAG+AGG 0.507344 2:-77076342 None:intergenic
TATTAAGAGCAATGAGGTAG+TGG 0.510345 2:+77077700 MsaT010724.1:CDS
ACAACTGGATGCTTCAAGGT+TGG 0.515568 2:+77078179 MsaT010724.1:CDS
GTTTCCTCTATTCTCTCTGC+CGG 0.517130 2:-77077575 None:intergenic
GTCTCATGAGCCTAAGGGTA+GGG 0.518003 2:+77076728 MsaT010724.1:CDS
TCCCCAAGCTGGCTTTCGAC+CGG 0.518500 2:+77077556 MsaT010724.1:CDS
CCATTACCTCCTTATCCAGT+GGG 0.519924 2:-77078096 None:intergenic
ACGAGTGGCTCCGAATTGGA+TGG 0.521140 2:-77076138 None:intergenic
GCCGCTGTGTGCTGAACAAA+AGG 0.522967 2:+77076998 MsaT010724.1:CDS
AGGCCGAATTACGTATGCTT+CGG 0.526366 2:+77078204 MsaT010724.1:CDS
CTCTGTGATGCATCACCTGA+AGG 0.529263 2:-77077212 None:intergenic
TCAGTAACATATCATTATGC+TGG 0.533464 2:-77078238 None:intergenic
AGGTGATGCATCACAGAGAA+TGG 0.535230 2:+77077217 MsaT010724.1:CDS
TACCAAAATCAATAATGTGA+AGG 0.537288 2:-77077442 None:intergenic
GCGATTGAAGCGCGCATGGT+TGG 0.539201 2:+77077257 MsaT010724.1:CDS
CATGAGCCTAAGGGTAGGGT+TGG 0.539617 2:+77076732 MsaT010724.1:CDS
AAAGAAACAAGGGAGGAAGA+AGG 0.539726 2:+77077082 MsaT010724.1:CDS
ACCAAAATCAATAATGTGAA+GGG 0.539883 2:-77077441 None:intergenic
TCAGTATGTTGAAGACTAAG+TGG 0.541144 2:-77076285 None:intergenic
GAAGCGCGCATGGTTGGTGC+TGG 0.541418 2:+77077263 MsaT010724.1:CDS
GCCATTACCTCCTTATCCAG+TGG 0.541967 2:-77078097 None:intergenic
CCACCCAAGTTGAGGATCAC+AGG 0.542256 2:+77077524 MsaT010724.1:CDS
CATATATAATATTTGTGTAC+CGG 0.543480 2:-77077288 None:intergenic
TAGGGTTGGAGTGATCAAGA+TGG 0.543815 2:+77076746 MsaT010724.1:CDS
GGAATTGAGTATCCCCAAGC+TGG 0.546615 2:+77077545 MsaT010724.1:CDS
GTATCTTATATGGTTTCCAG+TGG 0.547028 2:+77077462 MsaT010724.1:CDS
GGTAGTGGCTGTGAACTGTT+TGG 0.549486 2:+77077715 MsaT010724.1:CDS
AGTAATAGTAATATTAGTGA+TGG 0.551444 2:+77076540 MsaT010724.1:CDS
GGTTGGAGTGATCAAGATGG+AGG 0.554472 2:+77076749 MsaT010724.1:CDS
TGAAGCGTGTGGAAAAGAAA+GGG 0.554500 2:-77077865 None:intergenic
CTTGTGCACAAAGAACCAAA+AGG 0.555209 2:-77077124 None:intergenic
CGAGACATACAAACAGTGGC+AGG 0.561859 2:+77078042 MsaT010724.1:CDS
ACCATGACCAACCCTTATGT+GGG 0.562138 2:+77076114 MsaT010724.1:CDS
CAGGTTAGGAATCTAAGGGC+TGG 0.564666 2:+77078061 MsaT010724.1:CDS
GGCCATACCATCCCGAAACT+GGG 0.564944 2:+77077652 MsaT010724.1:CDS
ATTCCTGTGATCCTCAACTT+GGG 0.566789 2:-77077527 None:intergenic
TCGACCGGCAGAGAGAATAG+AGG 0.567756 2:+77077571 MsaT010724.1:CDS
ACTGGTGTATCAGGTGAAAG+AGG 0.568315 2:-77076447 None:intergenic
ACTTAATAAGAATTGGCCAC+TGG 0.572363 2:-77077478 None:intergenic
CAAGAAAGTGACGATGAAGA+AGG 0.574577 2:+77076888 MsaT010724.1:CDS
CTACAACATTCATAATCTCC+CGG 0.577244 2:-77077988 None:intergenic
ATGAATGTTGTAGCATGTGA+AGG 0.579064 2:+77077998 MsaT010724.1:CDS
TTGTGCACAAAGAACCAAAA+GGG 0.580206 2:-77077123 None:intergenic
ATGGTTTCCCAGTTTCGGGA+TGG 0.581937 2:-77077659 None:intergenic
CTGTGTGCTGAACAAAAGGA+TGG 0.582319 2:+77077002 MsaT010724.1:CDS
CAGGTTGGTGAGCTTGATGG+AGG 0.586798 2:+77077047 MsaT010724.1:CDS
TTAGAGATAGGCTAAGAGAA+TGG 0.591729 2:+77078125 MsaT010724.1:CDS
GGATGGGCCCACATAAGGGT+TGG 0.592672 2:-77076121 None:intergenic
CCTGTGATCCTCAACTTGGG+TGG 0.597902 2:-77077524 None:intergenic
TGGTGAGCTTGATGGAGGGA+AGG 0.598425 2:+77077052 MsaT010724.1:CDS
GGAATCTCAAGAACATCAAG+AGG 0.598639 2:-77076363 None:intergenic
TGGATGCTTCAAGGTTGGAA+AGG 0.598660 2:+77078184 MsaT010724.1:CDS
CGAGTGGCTCCGAATTGGAT+GGG 0.599028 2:-77076137 None:intergenic
GTTGGAGTGATCAAGATGGA+GGG 0.607035 2:+77076750 MsaT010724.1:CDS
GAACTATTACCAGAATCAGT+AGG 0.607462 2:-77076405 None:intergenic
GGAGTGATCAAGATGGAGGG+TGG 0.608714 2:+77076753 MsaT010724.1:CDS
AAGTGACTCCTTGAAGCGTG+TGG 0.608768 2:-77077876 None:intergenic
GAATGCGATTGAAGCGCGCA+TGG 0.609128 2:+77077253 MsaT010724.1:CDS
TGTCTCATGAGCCTAAGGGT+AGG 0.616168 2:+77076727 MsaT010724.1:CDS
CAGTATGTTGAAGACTAAGT+GGG 0.617757 2:-77076284 None:intergenic
GCCCACATAAGGGTTGGTCA+TGG 0.617923 2:-77076115 None:intergenic
TGGTATGGCCTTGTATTCGA+AGG 0.618500 2:-77077639 None:intergenic
AGATATTTCACTCTCCTCAA+CGG 0.618626 2:-77076937 None:intergenic
AGTGGTGGAAAGTAGAACTC+AGG 0.620773 2:+77077028 MsaT010724.1:CDS
AAGGCACATACAAAGAAACA+AGG 0.622167 2:+77077071 MsaT010724.1:CDS
GCCGGTCGAAAGCCAGCTTG+GGG 0.629257 2:-77077557 None:intergenic
GCAGTCTGCGGTTTCCGTTG+AGG 0.630810 2:+77076923 MsaT010724.1:CDS
ACAAAAGGATGGATCAGTGG+TGG 0.631801 2:+77077013 MsaT010724.1:CDS
AGGTTGGTGAGCTTGATGGA+GGG 0.634801 2:+77077048 MsaT010724.1:CDS
AGAGAATAGAGGAAACTGGC+CGG 0.638860 2:+77077582 MsaT010724.1:CDS
GCAACTCCCACTGGATAAGG+AGG 0.639677 2:+77078090 MsaT010724.1:CDS
CACAATAGTTAGCTAAACGC+CGG 0.640868 2:-77077601 None:intergenic
ACCTACAAATTCGATGACCA+TGG 0.643298 2:+77076045 MsaT010724.1:CDS
ATCACTCCAACCCTACCCTT+AGG 0.645813 2:-77076738 None:intergenic
TTAGTGTCTCATGAGCCTAA+GGG 0.648279 2:+77076723 MsaT010724.1:CDS
GTGGCAGGTTAGGAATCTAA+GGG 0.650267 2:+77078057 MsaT010724.1:CDS
AGAGAAGAATTAGATTACCA+TGG 0.658943 2:-77076062 None:intergenic
GGACTGCTAATGAATTGTTG+AGG 0.659007 2:+77077168 MsaT010724.1:CDS
CCTGATACACCAGTTTCTGG+TGG 0.666329 2:+77076456 MsaT010724.1:CDS
GTGGAAAGTAGAACTCAGGT+TGG 0.667091 2:+77077032 MsaT010724.1:CDS
AGTGGGTCATAACACATGCA+TGG 0.667506 2:-77076267 None:intergenic
AGGGTGGACCACCCAAGTTG+AGG 0.670566 2:+77077516 MsaT010724.1:CDS
GAAGCGTGTGGAAAAGAAAG+GGG 0.671904 2:-77077864 None:intergenic
GGCCCGAGACATACAAACAG+TGG 0.676458 2:+77078038 MsaT010724.1:CDS
CCTTAATATTAAGAGCAATG+AGG 0.678676 2:+77077694 MsaT010724.1:CDS
AAGAAGTAACAAGCAGTCTG+CGG 0.680051 2:+77076911 MsaT010724.1:CDS
GAATCTCAAGAACATCAAGA+GGG 0.681038 2:-77076362 None:intergenic
CACATACAAAGAAACAAGGG+AGG 0.681274 2:+77077075 MsaT010724.1:CDS
ATGGATTGAACAAATATGCG+CGG 0.684340 2:-77077821 None:intergenic
AGGGAGGAAGAAGGAAACTG+TGG 0.685784 2:+77077091 MsaT010724.1:CDS
TGAACAAAAGGATGGATCAG+TGG 0.692488 2:+77077010 MsaT010724.1:CDS
AGGCACATACAAAGAAACAA+GGG 0.704355 2:+77077072 MsaT010724.1:CDS
TATCCCGTGAAAATGAATGG+AGG 0.724072 2:+77077939 MsaT010724.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 77076015 77078246 77076015 ID=MsaG010724
Chr2 mRNA 77076015 77078246 77076015 ID=MsaT010724.1;Parent=MsaG010724
Chr2 exon 77076015 77078246 77076015 ID=MsaT010724.1.exon1;Parent=MsaT010724.1
Chr2 CDS 77076015 77078246 77076015 ID=cds.MsaT010724.1;Parent=MsaT010724.1
Gene Sequence

>MsaT010724.1

ATGGATCCGAACTTTGTCGGATTCGAAACAACCTACAAATTCGATGACCATGGTAATCTAATTCTTCTCTCTGATCCAAATCCAAACCTTAACGATTTCACCATGACCAACCCTTATGTGGGCCCATCCAATTCGGAGCCACTCGTTTTTTCTTCTGATGCAACCAAAGATTCTACCTTTGAAGATGCTGATTTTTCAGAAACTGTGAAATATATAAGCCAGATTCTAATGGAAGAAGATTTTGAACAAAAACCATGCATGTGTTATGACCCACTTAGTCTTCAACATACTGAGAAAATTTTCTTTGATGCTTTGGAATCAAATTTGCCTCTTTCACCTAATCAACACCCTCTTGATGTTCTTGAGATTCCTAGTAGCAATTGTTGTGTTCCTACTGATTCTGGTAATAGTTCTAGTTCTAATGAGTTAAAGCCTCTTTCACCTGATACACCAGTTTCTGGTGGTGATTCTGGTTTTAATTCAGTGAAGTCTCAGTTTATTGTTCCAAATCATGGTTTCAGTTTGAGTAATAGTAATATTAGTGATGGTGTTTTTGATTTGGATTCTTCTGAGACTAGGGTTATGGCTCAGAATATTTTTAGTGATGCTGATTCTATTTTGCAGTTTAGAAAAGGGTTAGAGGAAGCTAGCAAGTTTCTTCCTCAAAAAGCTCAGCTTTTTACTGGTTTTGAGAATGCTAGTGATAGTTTAGTGTCTCATGAGCCTAAGGGTAGGGTTGGAGTGATCAAGATGGAGGGTGGTGTTAGGGAGAACTCGAATTCGAGTTATTCAAGTTCAAATTTGAATTCGGGTTATTCGAGTTCAAATTCGAATTCAAATGGTTTGTTGAAGAGTAGAAAGAATCACGAGCGTCAAGAAAGTGACGATGAAGAAGGAAGAAGTAACAAGCAGTCTGCGGTTTCCGTTGAGGAGAGTGAAATATCTGATATGTTTGATAGGGTTTTGCTTAGTGTTGAAAACGTGCCGCTGTGTGCTGAACAAAAGGATGGATCAGTGGTGGAAAGTAGAACTCAGGTTGGTGAGCTTGATGGAGGGAAGGCACATACAAAGAAACAAGGGAGGAAGAAGGAAACTGTGGATTTGAGGACCCTTTTGGTTCTTTGTGCACAAGCTGTGTCTGCTAATGACAATAGGACTGCTAATGAATTGTTGAGGCAGATTAGGAAGCATTCTTCTCCTTCAGGTGATGCATCACAGAGAATGGCTCATTACTTTGCGAATGCGATTGAAGCGCGCATGGTTGGTGCTGGTACCGGTACACAAATATTATATATGAGCCAAAAGATGTTTAGTGCTGCTGATTTCTTGAAAGCTTACCAAGTTTTTATCTCTGCTTGCCCTTTCAAAAAATTTGCACATTTCTTTGCAAATAAAATGATTTTGAAAACAGCTGAAAAGGCAGAAACCCTTCACATTATTGATTTTGGTATCTTATATGGTTTCCAGTGGCCAATTCTTATTAAGTTTTTGTCAAAAGTAGAGGGTGGACCACCCAAGTTGAGGATCACAGGAATTGAGTATCCCCAAGCTGGCTTTCGACCGGCAGAGAGAATAGAGGAAACTGGCCGGCGTTTAGCTAACTATTGTGAACGTTTCAATGTTTCCTTCGAATACAAGGCCATACCATCCCGAAACTGGGAAACCATTCAAATTGAAGACCTTAATATTAAGAGCAATGAGGTAGTGGCTGTGAACTGTTTGGTAAGGTTTAAGAATTTACATGATGAGACAATCGACGTGAACAGTCCTAAAGATGCAGTTCTGAAGTTAATCAGGAAGATAAATCCGCGCATATTTGTTCAATCCATAGTAAATGGGTCTTATAATGCCCCTTTCTTTTCCACACGCTTCAAGGAGTCACTTTTCCATTATTCTGCAATGTTTGATATGTATGATACTCTCATATCCCGTGAAAATGAATGGAGGTCGATGATTGAGAGAGAGTTTTTGGGCCGGGAGATTATGAATGTTGTAGCATGTGAAGGTTTTGAGAGGGTTGAGAGGCCCGAGACATACAAACAGTGGCAGGTTAGGAATCTAAGGGCTGGCTTTAGGCAACTCCCACTGGATAAGGAGGTAATGGCCAGATTTAGAGATAGGCTAAGAGAATGGTACCATAAAGATTTTGTCTTTGATGAAGATAACAACTGGATGCTTCAAGGTTGGAAAGGCCGAATTACGTATGCTTCGGCTGGTTGGGTGCCAGCATAA
Protein sequence

>MsaT010724.1

MDPNFVGFETTYKFDDHGNLILLSDPNPNLNDFTMTNPYVGPSNSEPLVFSSDATKDSTFEDADFSETVKYISQILMEEDFEQKPCMCYDPLSLQHTEKIFFDALESNLPLSPNQHPLDVLEIPSSNCCVPTDSGNSSSSNELKPLSPDTPVSGGDSGFNSVKSQFIVPNHGFSLSNSNISDGVFDLDSSETRVMAQNIFSDADSILQFRKGLEEASKFLPQKAQLFTGFENASDSLVSHEPKGRVGVIKMEGGVRENSNSSYSSSNLNSGYSSSNSNSNGLLKSRKNHERQESDDEEGRSNKQSAVSVEESEISDMFDRVLLSVENVPLCAEQKDGSVVESRTQVGELDGGKAHTKKQGRKKETVDLRTLLVLCAQAVSANDNRTANELLRQIRKHSSPSGDASQRMAHYFANAIEARMVGAGTGTQILYMSQKMFSAADFLKAYQVFISACPFKKFAHFFANKMILKTAEKAETLHIIDFGILYGFQWPILIKFLSKVEGGPPKLRITGIEYPQAGFRPAERIEETGRRLANYCERFNVSFEYKAIPSRNWETIQIEDLNIKSNEVVAVNCLVRFKNLHDETIDVNSPKDAVLKLIRKINPRIFVQSIVNGSYNAPFFSTRFKESLFHYSAMFDMYDTLISRENEWRSMIEREFLGREIMNVVACEGFERVERPETYKQWQVRNLRAGFRQLPLDKEVMARFRDRLREWYHKDFVFDEDNNWMLQGWKGRITYASAGWVPA*