AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010725

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010725 MtrunA17_Chr2g0328401 93.909 591 34 1 7 597 48 636 0.0 1155
MsaG010725 MtrunA17_Chr2g0328431 81.361 617 112 2 2 618 43 656 0.0 1049
MsaG010725 MtrunA17_Chr2g0328411 82.055 613 107 2 4 616 45 654 0.0 1045
MsaG010725 MtrunA17_Chr4g0031741 58.544 632 196 12 7 618 67 652 0.0 694
MsaG010725 MtrunA17_Chr7g0238891 43.658 678 307 13 9 618 72 742 0.0 530
MsaG010725 MtrunA17_Chr4g0031721 43.505 662 313 12 9 618 34 686 1.23e-180 527
MsaG010725 MtrunA17_Chr4g0031701 45.787 629 281 14 14 618 36 628 4.05e-168 493
MsaG010725 MtrunA17_Chr4g0031711 44.848 660 288 17 15 618 96 735 1.07e-167 496
MsaG010725 MtrunA17_Chr4g0031731 45.310 629 284 14 14 618 36 628 1.63e-164 484
MsaG010725 MtrunA17_Chr2g0328391 51.288 466 213 5 161 618 212 671 2.92e-163 482
MsaG010725 MtrunA17_Chr2g0328381 45.958 433 221 5 191 618 219 643 1.53e-135 410
MsaG010725 MtrunA17_Chr2g0328341 46.207 435 216 7 191 618 217 640 8.83e-135 408
MsaG010725 MtrunA17_Chr2g0328361 42.166 434 211 7 191 618 218 617 1.68e-116 360
MsaG010725 MtrunA17_Chr2g0328371 50.000 256 123 2 318 572 1 252 2.30e-91 282
MsaG010725 MtrunA17_Chr2g0290241 29.144 374 260 3 246 615 52 424 6.00e-55 192
MsaG010725 MtrunA17_Chr5g0447691 32.172 373 247 5 247 615 173 543 6.35e-55 195
MsaG010725 MtrunA17_Chr2g0319091 32.172 373 247 5 247 615 208 578 1.82e-52 189
MsaG010725 MtrunA17_Chr4g0076091 29.167 384 257 6 242 616 166 543 8.94e-52 187
MsaG010725 MtrunA17_Chr1g0159461 27.989 368 261 2 251 615 225 591 8.43e-49 179
MsaG010725 MtrunA17_Chr7g0224271 27.949 390 249 4 246 615 295 672 2.40e-48 179
MsaG010725 MtrunA17_Chr3g0125811 29.111 371 253 6 251 615 203 569 5.66e-48 177
MsaG010725 MtrunA17_Chr3g0101141 29.333 375 255 5 247 615 171 541 1.16e-47 175
MsaG010725 MtrunA17_Chr5g0445331 29.815 379 252 8 247 615 157 531 1.23e-46 172
MsaG010725 MtrunA17_Chr8g0357601 28.941 387 246 6 246 615 177 551 3.85e-46 171
MsaG010725 MtrunA17_Chr8g0376381 29.620 368 239 8 252 615 170 521 1.04e-45 169
MsaG010725 MtrunA17_Chr4g0057891 27.532 385 251 7 248 615 146 519 7.47e-45 167
MsaG010725 MtrunA17_Chr7g0245601 29.620 368 240 7 254 617 441 793 2.62e-43 166
MsaG010725 MtrunA17_Chr3g0110971 28.535 389 247 7 244 615 212 586 1.27e-42 162
MsaG010725 MtrunA17_Chr2g0306641 30.000 390 249 12 238 615 109 486 1.02e-41 157
MsaG010725 MtrunA17_Chr4g0057291 24.611 386 266 9 247 617 288 663 4.59e-38 149
MsaG010725 MtrunA17_Chr4g0038851 25.995 427 248 6 251 615 48 468 8.72e-38 146
MsaG010725 MtrunA17_Chr4g0038761 27.926 376 242 8 254 618 87 444 9.02e-38 145
MsaG010725 MtrunA17_Chr3g0085681 26.859 417 245 7 252 615 26 435 3.09e-37 144
MsaG010725 MtrunA17_Chr2g0294461 27.411 394 248 10 246 615 205 584 5.68e-36 142
MsaG010725 MtrunA17_Chr4g0039661 25.729 377 259 7 248 615 190 554 7.00e-33 133
MsaG010725 MtrunA17_Chr1g0200401 27.020 396 247 11 248 615 77 458 8.19e-31 125
MsaG010725 MtrunA17_Chr7g0270031 27.273 385 250 10 246 615 184 553 9.22e-31 127
MsaG010725 MtrunA17_Chr5g0401131 25.459 381 267 7 251 617 114 491 2.09e-29 122
MsaG010725 MtrunA17_Chr1g0183641 26.913 379 250 13 246 616 118 477 4.92e-29 120
MsaG010725 MtrunA17_Chr2g0294471 25.063 395 253 10 246 615 199 575 5.27e-29 122
MsaG010725 MtrunA17_Chr3g0114841 29.968 317 185 11 317 616 204 500 1.23e-27 117
MsaG010725 MtrunA17_Chr5g0396491 30.622 209 135 3 251 455 56 258 8.76e-25 108
MsaG010725 MtrunA17_Chr5g0396491 28.859 149 106 0 467 615 329 477 7.04e-16 80.9
MsaG010725 MtrunA17_Chr7g0236851 28.137 263 179 5 355 615 402 656 1.46e-24 108
MsaG010725 MtrunA17_Chr1g0194621 23.096 394 262 10 246 615 141 517 2.63e-24 107
MsaG010725 MtrunA17_Chr5g0424131 24.791 359 240 11 278 616 151 499 8.17e-24 105
MsaG010725 MtrunA17_Chr5g0401421 22.482 427 277 10 241 618 27 448 1.23e-23 104
MsaG010725 MtrunA17_Chr3g0085361 25.284 352 206 5 313 615 6 349 4.36e-23 101
MsaG010725 MtrunA17_Chr6g0468561 26.715 277 185 6 348 615 394 661 4.89e-23 103
MsaG010725 MtrunA17_Chr4g0053901 22.837 416 269 9 251 618 59 470 6.31e-22 99.4
MsaG010725 MtrunA17_Chr2g0329591 25.000 392 256 11 251 617 74 452 1.36e-21 98.2
MsaG010725 MtrunA17_Chr8g0383981 23.707 464 289 21 195 618 60 498 3.39e-21 97.4
MsaG010725 MtrunA17_Chr7g0230731 22.432 370 266 7 252 615 373 727 1.42e-15 80.5
MsaG010725 MtrunA17_Chr7g0230771 21.739 368 271 6 252 615 376 730 2.20e-14 76.6
MsaG010725 MtrunA17_Chr4g0012071 19.950 401 286 12 229 615 171 550 5.27e-13 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010725 AT2G29060.2 43.429 624 315 13 9 617 56 656 2.43e-165 488
MsaG010725 AT2G37650.1 41.960 653 320 12 9 617 77 714 4.33e-164 486
MsaG010725 AT2G37650.2 41.960 653 320 12 9 617 65 702 4.48e-164 486
MsaG010725 AT2G29060.1 42.219 649 324 13 9 617 56 693 4.01e-163 483
MsaG010725 AT1G07530.1 49.145 468 223 4 163 618 303 767 2.31e-161 481
MsaG010725 AT5G59450.1 45.826 539 248 10 114 618 72 600 1.01e-160 474
MsaG010725 AT3G46600.3 50.559 447 202 7 178 618 116 549 4.76e-149 442
MsaG010725 AT3G46600.1 50.559 447 202 7 178 618 148 581 6.81e-149 442
MsaG010725 AT2G29065.1 42.492 626 314 15 10 618 34 630 5.14e-147 439
MsaG010725 AT3G46600.2 54.839 372 162 2 251 618 4 373 5.08e-146 427
MsaG010725 AT1G07520.2 46.488 484 240 9 145 618 57 531 6.08e-138 413
MsaG010725 AT1G07520.4 46.488 484 240 9 145 618 57 531 6.08e-138 413
MsaG010725 AT1G07520.1 46.488 484 240 9 145 618 221 695 3.04e-136 414
MsaG010725 AT1G07520.3 46.488 484 240 9 145 618 222 696 3.13e-136 414
MsaG010725 AT1G50600.3 32.796 372 246 3 247 615 155 525 2.09e-54 194
MsaG010725 AT1G50600.1 32.796 372 246 3 247 615 226 596 9.29e-54 194
MsaG010725 AT1G50600.2 32.796 372 246 3 247 615 230 600 1.12e-53 193
MsaG010725 AT5G48150.1 30.105 382 262 3 238 615 109 489 6.37e-53 189
MsaG010725 AT5G48150.4 30.105 382 262 3 238 615 109 489 6.37e-53 189
MsaG010725 AT5G48150.2 30.105 382 262 3 238 615 109 489 6.37e-53 189
MsaG010725 AT5G48150.3 30.105 382 262 3 238 615 109 489 6.37e-53 189
MsaG010725 AT2G04890.1 29.839 372 250 4 247 615 49 412 1.34e-49 178
MsaG010725 AT4G17230.1 29.600 375 260 2 246 617 153 526 1.13e-46 172
MsaG010725 AT4G17230.2 29.600 375 260 2 246 617 153 526 1.13e-46 172
MsaG010725 AT1G21450.1 27.027 407 290 4 214 615 188 592 1.20e-45 171
MsaG010725 AT3G54220.1 31.283 374 229 11 254 617 296 651 9.33e-44 166
MsaG010725 AT3G03450.1 28.684 380 252 7 244 615 176 544 5.88e-43 162
MsaG010725 AT5G17490.1 27.356 435 291 9 189 615 98 515 9.52e-42 159
MsaG010725 AT1G66350.1 29.902 408 242 13 226 615 124 505 2.77e-41 157
MsaG010725 AT1G14920.1 28.877 374 250 9 246 615 167 528 9.85e-41 156
MsaG010725 AT5G66770.1 27.346 373 260 7 248 615 217 583 1.58e-39 153
MsaG010725 AT1G50420.1 24.885 434 251 6 248 615 54 478 1.72e-39 151
MsaG010725 AT2G01570.1 27.273 374 256 9 246 615 219 580 3.78e-37 146
MsaG010725 AT5G41920.1 26.546 388 251 8 241 615 33 399 8.23e-37 142
MsaG010725 AT1G63100.1 26.515 396 251 8 247 616 248 629 7.40e-34 137
MsaG010725 AT1G63100.2 26.515 396 251 8 247 616 273 654 1.15e-33 137
MsaG010725 AT3G50650.1 25.352 355 251 5 269 615 193 541 1.44e-33 135
MsaG010725 AT4G37650.1 25.255 392 263 8 250 617 145 530 3.94e-33 134
MsaG010725 AT4G08250.1 28.846 364 234 13 267 616 125 477 1.43e-30 125
MsaG010725 AT3G49950.1 27.551 392 247 11 251 617 30 409 2.08e-29 121
MsaG010725 AT5G52510.1 25.326 383 255 11 252 615 269 639 1.32e-28 121
MsaG010725 AT1G55580.1 24.289 387 227 10 280 615 73 444 1.78e-24 107
MsaG010725 AT3G13840.1 23.544 412 267 16 226 615 124 509 2.79e-15 79.3
MsaG010725 AT4G00150.1 21.839 435 285 13 195 615 160 553 3.33e-11 66.6

Find 126 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGATCCAGATGGAAATTT+TGG 0.205044 2:+77086174 MsaT010725.1:CDS
CATCGACTACAAAATCTTTA+TGG 0.219672 2:-77087742 None:intergenic
CCTGACCTGCCATTGCTTGT+AGG 0.253220 2:-77087639 None:intergenic
TCCAAGTACCATTCTGAAAA+TGG 0.263904 2:-77086383 None:intergenic
AGTGTTCATATTATTGATTT+TGG 0.276562 2:+77087035 MsaT010725.1:CDS
AGGCAACACTCTTCGCCTTT+TGG 0.276975 2:+77086804 MsaT010725.1:CDS
TATGTATCTGAGGATGTTAT+TGG 0.308200 2:-77086010 None:intergenic
TTCCTTCTTCACCATTGCTT+TGG 0.317965 2:-77087717 None:intergenic
AGCTTCCTCAGCCAGGATTT+AGG 0.323032 2:+77087147 MsaT010725.1:CDS
CACTAAGCTGTAAATTGTAT+TGG 0.330543 2:-77087000 None:intergenic
GTATCATCATATGATGAATT+AGG 0.332225 2:-77086150 None:intergenic
TTTGTATGCTTTATCTGCTT+GGG 0.337273 2:+77087805 MsaT010725.1:CDS
CCCGTAATTCGAAGCTTTGC+AGG 0.346583 2:-77087119 None:intergenic
CGAATTTGACTCTTCCTACT+TGG 0.356470 2:+77086278 MsaT010725.1:CDS
GTTTCCTCTGCCCTTTCTGC+AGG 0.358323 2:-77087170 None:intergenic
ACAACTGCATTGCACAGAAA+TGG 0.365582 2:+77087243 MsaT010725.1:CDS
GCATCACATAATGTTGATTC+AGG 0.366614 2:+77086630 MsaT010725.1:CDS
CTCTCAAGCATCAGCCTTTG+CGG 0.367574 2:-77087545 None:intergenic
TTGGATTAATCTTCCTGATC+AGG 0.372735 2:-77087394 None:intergenic
TTAGCTCATTACTTTGCTAA+TGG 0.378914 2:+77086843 MsaT010725.1:CDS
GCAGAAAGGGCAGAGGAAAC+TGG 0.381946 2:+77087173 MsaT010725.1:CDS
TCCTGCAAAGCTTCGAATTA+CGG 0.385651 2:+77087118 MsaT010725.1:CDS
TCTGATTCGGTTAACCTTGT+TGG 0.387152 2:+77086255 MsaT010725.1:CDS
CCTGCAAAGCTTCGAATTAC+GGG 0.388387 2:+77087119 MsaT010725.1:CDS
TCTCAAGCATCAGCCTTTGC+GGG 0.391520 2:-77087544 None:intergenic
GCGCAAACCCTGCATGTTGC+AGG 0.405053 2:+77086068 MsaT010725.1:CDS
GAGCTAGGTTCAAGCAAATC+AGG 0.411714 2:+77087669 MsaT010725.1:CDS
TTCTTCCATGGAGTTGTCAA+TGG 0.415847 2:+77087422 MsaT010725.1:CDS
ATGCTTGAGAGAGGGTTGTT+TGG 0.419092 2:+77087557 MsaT010725.1:CDS
TGCCATTGCTTGTAGGTCTC+AGG 0.423545 2:-77087632 None:intergenic
CAACATTATGTGATGCTTGT+TGG 0.426130 2:-77086623 None:intergenic
TTAGTAGAGCAAAACTACTA+TGG 0.426979 2:+77086315 MsaT010725.1:CDS
ATGAGCTACTCAAGCAGATT+AGG 0.437525 2:+77086784 MsaT010725.1:CDS
ATCGTTGTTTGATATGTTTG+AGG 0.437931 2:+77087502 MsaT010725.1:CDS
CACAGAAATGGGAAACTATC+CGG 0.438062 2:+77087255 MsaT010725.1:CDS
CAACTGCATTGCACAGAAAT+GGG 0.438307 2:+77087244 MsaT010725.1:CDS
CCTACAAGCAATGGCAGGTC+AGG 0.440693 2:+77087639 MsaT010725.1:CDS
AAGCATTGTTGAACCTGATC+AGG 0.443595 2:+77087381 MsaT010725.1:CDS
AGGATTTAGGCCTGCAGAAA+GGG 0.451557 2:+77087160 MsaT010725.1:CDS
TTCAGAATGGTACTTGGAGT+TGG 0.458122 2:+77086388 MsaT010725.1:CDS
AGGTTCAACAATGCTTCTCT+TGG 0.460604 2:-77087374 None:intergenic
TTCACTGCTACAGTTTCATC+AGG 0.464564 2:-77087347 None:intergenic
CTTTCTGCAGGCCTAAATCC+TGG 0.464941 2:-77087158 None:intergenic
CATGGATGAAGAAGATGACT+TGG 0.465816 2:+77086044 MsaT010725.1:CDS
GTGCTTCCCTAAACCGAGTC+AGG 0.469223 2:-77087466 None:intergenic
TTGAGAGAGGGTTGTTTGGA+AGG 0.472118 2:+77087561 MsaT010725.1:CDS
AACTCAATGTGCACAGGCCG+TGG 0.475370 2:+77086737 MsaT010725.1:CDS
TAAACATTCCTGCAACATGC+AGG 0.476644 2:-77086076 None:intergenic
TAGTCGATGTCGATGGTAAG+TGG 0.481602 2:+77087756 MsaT010725.1:CDS
ATGCCACAGTTGTGGATTTG+TGG 0.483676 2:+77086706 MsaT010725.1:CDS
TAATTCATGTGAGTCTGATT+CGG 0.485201 2:+77086242 MsaT010725.1:CDS
CGCTGAAGTCCATCCCCAAA+AGG 0.487864 2:-77086819 None:intergenic
TACTTCTTTGTCAGCAAGGA+AGG 0.488696 2:+77086586 MsaT010725.1:CDS
AGCAATGGCAGGTCAGGAAC+AGG 0.489352 2:+77087645 MsaT010725.1:CDS
TGCTCAATGAGCACTGGATC+AGG 0.490813 2:-77086348 None:intergenic
AGTACCTCATCTAAGATCTC+TGG 0.492137 2:-77086567 None:intergenic
CGAAGCTTTGCAGGACCACC+AGG 0.497005 2:-77087110 None:intergenic
CCGCTTGATGTAGTAACTGC+TGG 0.498718 2:+77086906 MsaT010725.1:CDS
AACCAAAGCAATGGTGAAGA+AGG 0.500696 2:+77087715 MsaT010725.1:CDS
TGGAATATCTATTCCTGTGT+AGG 0.502750 2:-77085990 None:intergenic
CGGCATATACGATGGAATCT+CGG 0.504421 2:-77086886 None:intergenic
AGCTTGTGAGGGTGCGGAGA+GGG 0.504460 2:+77087601 MsaT010725.1:CDS
GTGCGGCCAAAATTTCCATC+TGG 0.507021 2:-77086180 None:intergenic
GGAATTGAGCTTCCTCAGCC+AGG 0.513281 2:+77087140 MsaT010725.1:CDS
CAGGATTTAGGCCTGCAGAA+AGG 0.516377 2:+77087159 MsaT010725.1:CDS
AGGTCAGGAACAGGAGAGCT+AGG 0.516508 2:+77087654 MsaT010725.1:CDS
TCGAGGATCTAAAGATAGAC+AGG 0.520782 2:+77087279 MsaT010725.1:CDS
AGCCTTTGCGGGTCTTCGCA+AGG 0.523484 2:-77087533 None:intergenic
AGGAGTTTGTAGTTCCAAGT+AGG 0.525291 2:-77086292 None:intergenic
GCTTGTTGCTCAATGAGCAC+TGG 0.529649 2:-77086354 None:intergenic
GAAACCTCTTCAACCATCAC+TGG 0.530989 2:-77086423 None:intergenic
CCTTTGATCGTAGCTTGCCA+CGG 0.533325 2:-77086754 None:intergenic
CCGTGGCAAGCTACGATCAA+AGG 0.538969 2:+77086754 MsaT010725.1:CDS
TGAGAGAGGGTTGTTTGGAA+GGG 0.539295 2:+77087562 MsaT010725.1:CDS
TACAAACAATGCCACAGTTG+TGG 0.553896 2:+77086698 MsaT010725.1:CDS
TCAGCCAGTGATGGTTGAAG+AGG 0.554665 2:+77086419 MsaT010725.1:CDS
CTGCTACAGTTTCATCAGGA+AGG 0.554851 2:-77087343 None:intergenic
TAATGTTGATTCAGGAGGAA+AGG 0.556753 2:+77086638 MsaT010725.1:CDS
GAATTTGCTATGGTTTCCAG+TGG 0.556961 2:+77087057 MsaT010725.1:CDS
AGAAAAGAGAAGTCATGTGA+TGG 0.557380 2:+77086464 MsaT010725.1:CDS
GCAATGCAGTTGTATTCAAA+TGG 0.559815 2:-77087233 None:intergenic
TGCCTTGCGAAGACCCGCAA+AGG 0.561465 2:+77087531 MsaT010725.1:CDS
ATACATAAGCGACATCCTCA+TGG 0.562884 2:+77086026 MsaT010725.1:CDS
TGTTATAGCTTGTGAGGGTG+CGG 0.569313 2:+77087595 MsaT010725.1:CDS
GAATCAAATTCAGCCAGTGA+TGG 0.572156 2:+77086410 MsaT010725.1:CDS
AAACATTCCTGCAACATGCA+GGG 0.581348 2:-77086075 None:intergenic
AACTTCCATTGACAACTCCA+TGG 0.582257 2:-77087427 None:intergenic
TATCAAACAACGATGAGAAG+TGG 0.582630 2:-77087493 None:intergenic
TGAGACCTACAAGCAATGGC+AGG 0.588661 2:+77087634 MsaT010725.1:CDS
GGCCTGAGACCTACAAGCAA+TGG 0.588665 2:+77087630 MsaT010725.1:CDS
TGATATTAGTAATGAACAAG+AGG 0.595603 2:+77086497 MsaT010725.1:CDS
ATATTCGTCATTCTCTGCAA+AGG 0.596904 2:-77086969 None:intergenic
TATAAATGTTATAGCTTGTG+AGG 0.597621 2:+77087589 MsaT010725.1:CDS
CAAAGGCTGATGCTTGAGAG+AGG 0.599769 2:+77087548 MsaT010725.1:CDS
AGTCGATGTCGATGGTAAGT+GGG 0.601905 2:+77087757 MsaT010725.1:CDS
GATGATACCACTGATCCAGA+TGG 0.602284 2:+77086165 MsaT010725.1:CDS
AAGAGTCAAATTCGCCAACA+AGG 0.602536 2:-77086269 None:intergenic
AGTGAATGAAACCAAAGCAA+TGG 0.607650 2:+77087706 MsaT010725.1:CDS
AGGGTGCGGAGAGGGTCGAA+AGG 0.608738 2:+77087609 MsaT010725.1:CDS
CTATCTTTAGATCCTCGAGC+CGG 0.610122 2:-77087274 None:intergenic
GAGGTACTTCTTTGTCAGCA+AGG 0.610885 2:+77086582 MsaT010725.1:CDS
TATGCTAACTCAATGTGCAC+AGG 0.610937 2:+77086731 MsaT010725.1:CDS
TAGGTTCAAGCAAATCAGGT+TGG 0.612445 2:+77087673 MsaT010725.1:CDS
CATTCTCTGCAAAGGAGATG+AGG 0.613851 2:-77086961 None:intergenic
ACCTCATCTAAGATCTCTGG+TGG 0.614764 2:-77086564 None:intergenic
GCAGGCCTAAATCCTGGCTG+AGG 0.614834 2:-77087152 None:intergenic
CCAGCAGTTACTACATCAAG+CGG 0.615147 2:-77086906 None:intergenic
TAGGCCTGCAGAAAGGGCAG+AGG 0.619921 2:+77087166 MsaT010725.1:CDS
AAAATTTCCATCTGGATCAG+TGG 0.623541 2:-77086172 None:intergenic
CTCAGATGAAGATCCTACAC+AGG 0.627955 2:+77085977 None:intergenic
AAAGGCTGATGCTTGAGAGA+GGG 0.635382 2:+77087549 MsaT010725.1:CDS
ATGGGAAACTATCCGGCTCG+AGG 0.640423 2:+77087262 MsaT010725.1:CDS
TCACATAATGTTGATTCAGG+AGG 0.642164 2:+77086633 MsaT010725.1:CDS
TAGCTTGTGAGGGTGCGGAG+AGG 0.646388 2:+77087600 MsaT010725.1:CDS
ACATCAAGCGGCATATACGA+TGG 0.651925 2:-77086894 None:intergenic
GATATTAGTAATGAACAAGA+GGG 0.653513 2:+77086498 MsaT010725.1:CDS
TGATTCAGGAGGAAAGGCAA+AGG 0.662454 2:+77086644 MsaT010725.1:CDS
ATAAATGTTATAGCTTGTGA+GGG 0.673348 2:+77087590 MsaT010725.1:CDS
AAACTTTCAATGAGACCTGG+TGG 0.675722 2:+77087095 MsaT010725.1:CDS
AGACTCACATGAATTATCAG+TGG 0.680494 2:-77086235 None:intergenic
AAAGGGCAGAGGAAACTGGA+AGG 0.686895 2:+77087177 MsaT010725.1:CDS
AGTCATCTTCTTCATCCATG+AGG 0.688746 2:-77086041 None:intergenic
ACCACCAGAGATCTTAGATG+AGG 0.690503 2:+77086563 MsaT010725.1:CDS
GGATGTCGCTTATGTATCTG+AGG 0.698731 2:-77086020 None:intergenic
AGTCCACAAATCCACAACTG+TGG 0.729336 2:-77086709 None:intergenic
TGCTCTCAAAACTAGCAGTG+CGG 0.748557 2:-77086197 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 77085982 77087838 77085982 ID=MsaG010725
Chr2 mRNA 77085982 77087838 77085982 ID=MsaT010725.1;Parent=MsaG010725
Chr2 exon 77085982 77087838 77085982 ID=MsaT010725.1.exon1;Parent=MsaT010725.1
Chr2 CDS 77085982 77087838 77085982 ID=cds.MsaT010725.1;Parent=MsaT010725.1
Gene Sequence

>MsaT010725.1

ATGAAGATCCTACACAGGAATAGATATTCCAATAACATCCTCAGATACATAAGCGACATCCTCATGGATGAAGAAGATGACTTGGAGCGCAAACCCTGCATGTTGCAGGAATGTTTAAAACTCCAAGCTGCTGAAAAATCATTCTATGATGTTCTTAGTCATAACAATCCTAATTCATCATATGATGATACCACTGATCCAGATGGAAATTTTGGCCGCACTGCTAGTTTTGAGAGCAATGGCAGCAGCTGCACCACTGATAATTCATGTGAGTCTGATTCGGTTAACCTTGTTGGCGAATTTGACTCTTCCTACTTGGAACTACAAACTCCTTTAGTAGAGCAAAACTACTATGGTGTGACACAACCTGATCCAGTGCTCATTGAGCAACAAGCTGCTAACCATTTTCAGAATGGTACTTGGAGTTGGAATCAAATTCAGCCAGTGATGGTTGAAGAGGTTTCACCTTCATTAGTACCAAGAGAAAAGAGAAGTCATGTGATGGATGATAACGATGATATTAGTAATGAACAAGAGGGAAGAGATAGCAAGATTTCAGCTATCTTTTTTGATGAATTAGAACCACCAGAGATCTTAGATGAGGTACTTCTTTGTCAGCAAGGAAGGACACTCACTCAACACCAACAAGCATCACATAATGTTGATTCAGGAGGAAAGGCAAAGGCAACACGTTCTCGATCGAAGAAAGTATCTACTACAAACAATGCCACAGTTGTGGATTTGTGGACTATGCTAACTCAATGTGCACAGGCCGTGGCAAGCTACGATCAAAGGAATACAAATGAGCTACTCAAGCAGATTAGGCAACACTCTTCGCCTTTTGGGGATGGACTTCAGCGGTTAGCTCATTACTTTGCTAATGGTCTTGAGATAAGATTTGCTGCCGAGATTCCATCGTATATGCCGCTTGATGTAGTAACTGCTGGAGATATGTTGAAAGCTTACAAACTATTCGTTACCTCATCTCCTTTGCAGAGAATGACGAATATGTTGTTAACCAATACAATTTACAGCTTAGTGAAGAACGAGAGCAGTGTTCATATTATTGATTTTGGAATTTGCTATGGTTTCCAGTGGCCATGCCTCATCAAAAAACTTTCAATGAGACCTGGTGGTCCTGCAAAGCTTCGAATTACGGGAATTGAGCTTCCTCAGCCAGGATTTAGGCCTGCAGAAAGGGCAGAGGAAACTGGAAGGCGTTTAGAGAACTACTGCAAGAAGTTCAATGTACCATTTGAATACAACTGCATTGCACAGAAATGGGAAACTATCCGGCTCGAGGATCTAAAGATAGACAGGAATGAAGTAACTCTTGTTAGCTGTTTATACAGAATGAAGAACCTTCCTGATGAAACTGTAGCAGTGAATTGTCCAAGAGAAGCATTGTTGAACCTGATCAGGAAGATTAATCCAAAAATCTTCTTCCATGGAGTTGTCAATGGAAGTTATAGTGCACCGTTTTTCCTGACTCGGTTTAGGGAAGCACTCTACCACTTCTCATCGTTGTTTGATATGTTTGAGGCTAACGTGCCTTGCGAAGACCCGCAAAGGCTGATGCTTGAGAGAGGGTTGTTTGGAAGGGATGCTATAAATGTTATAGCTTGTGAGGGTGCGGAGAGGGTCGAAAGGCCTGAGACCTACAAGCAATGGCAGGTCAGGAACAGGAGAGCTAGGTTCAAGCAAATCAGGTTGGATTCTGATTTAGTGAATGAAACCAAAGCAATGGTGAAGAAGGAATACCATAAAGATTTTGTAGTCGATGTCGATGGTAAGTGGGTTTTGCAGGGATGGAAAGGACGCATTTTGTATGCTTTATCTGCTTGGGTTCCTGCTTAA
Protein sequence

>MsaT010725.1

MKILHRNRYSNNILRYISDILMDEEDDLERKPCMLQECLKLQAAEKSFYDVLSHNNPNSSYDDTTDPDGNFGRTASFESNGSSCTTDNSCESDSVNLVGEFDSSYLELQTPLVEQNYYGVTQPDPVLIEQQAANHFQNGTWSWNQIQPVMVEEVSPSLVPREKRSHVMDDNDDISNEQEGRDSKISAIFFDELEPPEILDEVLLCQQGRTLTQHQQASHNVDSGGKAKATRSRSKKVSTTNNATVVDLWTMLTQCAQAVASYDQRNTNELLKQIRQHSSPFGDGLQRLAHYFANGLEIRFAAEIPSYMPLDVVTAGDMLKAYKLFVTSSPLQRMTNMLLTNTIYSLVKNESSVHIIDFGICYGFQWPCLIKKLSMRPGGPAKLRITGIELPQPGFRPAERAEETGRRLENYCKKFNVPFEYNCIAQKWETIRLEDLKIDRNEVTLVSCLYRMKNLPDETVAVNCPREALLNLIRKINPKIFFHGVVNGSYSAPFFLTRFREALYHFSSLFDMFEANVPCEDPQRLMLERGLFGRDAINVIACEGAERVERPETYKQWQVRNRRARFKQIRLDSDLVNETKAMVKKEYHKDFVVDVDGKWVLQGWKGRILYALSAWVPA*