AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010726

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010726 MtrunA17_Chr2g0328411 87.615 654 36 3 1 609 1 654 0.0 1143
MsaG010726 MtrunA17_Chr2g0328431 84.909 656 54 3 1 611 1 656 0.0 1120
MsaG010726 MtrunA17_Chr2g0328401 75.629 636 109 3 1 590 1 636 0.0 959
MsaG010726 MtrunA17_Chr4g0031741 52.474 667 246 13 1 611 1 652 0.0 641
MsaG010726 MtrunA17_Chr7g0238891 40.988 688 276 15 43 611 66 742 5.26e-163 484
MsaG010726 MtrunA17_Chr2g0328391 41.504 665 313 13 4 611 26 671 1.46e-156 465
MsaG010726 MtrunA17_Chr4g0031721 51.461 445 178 7 198 611 249 686 2.87e-151 452
MsaG010726 MtrunA17_Chr4g0031701 43.631 628 270 17 43 611 26 628 4.78e-151 449
MsaG010726 MtrunA17_Chr4g0031731 43.312 628 272 17 43 611 26 628 2.02e-147 440
MsaG010726 MtrunA17_Chr4g0031711 48.451 452 189 7 195 611 293 735 1.49e-144 436
MsaG010726 MtrunA17_Chr2g0328341 37.397 607 316 16 50 611 53 640 1.87e-126 386
MsaG010726 MtrunA17_Chr2g0328381 36.928 612 318 14 50 611 50 643 2.77e-125 383
MsaG010726 MtrunA17_Chr2g0328361 40.185 433 202 9 203 611 218 617 1.13e-102 324
MsaG010726 MtrunA17_Chr2g0328371 46.094 256 114 3 330 565 1 252 1.30e-78 249
MsaG010726 MtrunA17_Chr2g0290241 27.321 377 250 7 255 608 49 424 2.75e-47 171
MsaG010726 MtrunA17_Chr5g0447691 30.184 381 241 8 251 608 165 543 1.14e-46 172
MsaG010726 MtrunA17_Chr2g0319091 30.179 391 248 7 241 608 190 578 1.61e-45 170
MsaG010726 MtrunA17_Chr4g0076091 26.963 382 253 9 252 609 164 543 1.88e-43 163
MsaG010726 MtrunA17_Chr1g0159461 27.446 368 244 6 263 608 225 591 3.61e-42 160
MsaG010726 MtrunA17_Chr7g0224271 27.806 392 232 6 256 608 293 672 3.68e-42 161
MsaG010726 MtrunA17_Chr4g0057891 27.763 389 226 8 260 608 146 519 5.57e-42 159
MsaG010726 MtrunA17_Chr3g0101141 27.559 381 250 7 253 608 162 541 4.71e-41 156
MsaG010726 MtrunA17_Chr5g0445331 28.571 378 239 11 259 608 157 531 9.54e-40 152
MsaG010726 MtrunA17_Chr8g0376381 29.067 375 227 10 257 608 163 521 5.56e-39 150
MsaG010726 MtrunA17_Chr3g0125811 26.178 382 257 6 251 608 189 569 2.39e-38 149
MsaG010726 MtrunA17_Chr2g0306641 28.499 393 246 11 243 608 102 486 2.22e-37 145
MsaG010726 MtrunA17_Chr8g0357601 27.132 387 242 7 257 611 176 554 4.21e-37 145
MsaG010726 MtrunA17_Chr3g0110971 27.320 388 232 9 257 608 213 586 4.07e-35 140
MsaG010726 MtrunA17_Chr7g0245601 27.520 367 230 8 266 610 441 793 5.56e-35 141
MsaG010726 MtrunA17_Chr3g0085681 26.139 417 229 8 264 608 26 435 1.91e-34 135
MsaG010726 MtrunA17_Chr4g0038851 24.419 430 238 6 260 608 45 468 1.28e-31 128
MsaG010726 MtrunA17_Chr4g0038761 26.330 376 229 9 266 611 87 444 4.90e-30 123
MsaG010726 MtrunA17_Chr2g0294461 26.684 386 242 9 258 608 205 584 1.16e-29 124
MsaG010726 MtrunA17_Chr4g0057291 23.711 388 252 12 257 610 286 663 8.04e-29 122
MsaG010726 MtrunA17_Chr4g0039661 24.403 377 245 8 260 608 190 554 5.92e-24 106
MsaG010726 MtrunA17_Chr5g0401131 23.698 384 251 9 263 610 114 491 2.19e-23 103
MsaG010726 MtrunA17_Chr7g0270031 23.821 424 246 10 227 608 165 553 5.39e-23 103
MsaG010726 MtrunA17_Chr1g0183641 24.409 381 238 13 257 608 117 476 8.99e-23 102
MsaG010726 MtrunA17_Chr2g0294471 24.804 383 250 10 258 608 199 575 1.29e-22 102
MsaG010726 MtrunA17_Chr3g0085361 26.421 299 163 5 359 608 59 349 4.51e-22 98.2
MsaG010726 MtrunA17_Chr7g0236851 26.778 239 166 4 371 608 426 656 5.68e-22 100
MsaG010726 MtrunA17_Chr1g0200401 23.284 408 249 12 251 608 65 458 7.18e-21 95.9
MsaG010726 MtrunA17_Chr6g0468561 28.319 226 146 5 392 608 443 661 9.61e-21 96.7
MsaG010726 MtrunA17_Chr5g0424131 24.302 358 222 11 290 608 151 498 3.44e-19 91.3
MsaG010726 MtrunA17_Chr1g0194621 25.498 251 174 6 361 608 277 517 3.73e-19 91.3
MsaG010726 MtrunA17_Chr3g0114841 25.737 373 213 15 282 608 145 499 1.95e-18 89.0
MsaG010726 MtrunA17_Chr5g0401421 22.062 417 254 10 263 611 35 448 8.44e-18 86.3
MsaG010726 MtrunA17_Chr5g0396491 28.302 212 123 3 260 448 53 258 3.72e-17 84.7
MsaG010726 MtrunA17_Chr5g0396491 29.530 149 105 0 460 608 329 477 7.11e-17 84.0
MsaG010726 MtrunA17_Chr2g0329591 24.181 397 234 12 263 610 74 452 4.21e-16 81.3
MsaG010726 MtrunA17_Chr4g0053901 22.249 418 250 11 263 611 59 470 1.24e-15 79.7
MsaG010726 MtrunA17_Chr8g0383981 21.900 379 237 17 268 611 144 498 3.19e-15 78.6
MsaG010726 MtrunA17_Chr2g0323111 25.698 179 130 3 434 611 132 308 4.13e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010726 AT2G29060.2 42.787 610 292 15 49 610 56 656 9.26e-155 460
MsaG010726 AT5G59450.1 42.905 592 253 13 53 611 61 600 3.24e-150 447
MsaG010726 AT2G29060.1 41.113 647 287 17 49 610 56 693 5.50e-150 449
MsaG010726 AT2G37650.2 39.692 650 292 14 49 610 65 702 2.76e-145 437
MsaG010726 AT2G37650.1 39.692 650 292 14 49 610 77 714 4.70e-145 437
MsaG010726 AT1G07530.1 47.403 462 211 6 181 611 307 767 1.11e-140 427
MsaG010726 AT3G46600.3 51.621 401 168 3 237 611 149 549 2.22e-138 414
MsaG010726 AT3G46600.1 51.621 401 168 3 237 611 181 581 2.68e-138 415
MsaG010726 AT3G46600.2 53.514 370 151 2 263 611 4 373 3.99e-137 405
MsaG010726 AT2G29065.1 52.468 385 153 6 257 611 246 630 1.13e-131 400
MsaG010726 AT1G07520.2 46.782 404 186 6 237 611 128 531 2.07e-120 367
MsaG010726 AT1G07520.4 46.782 404 186 6 237 611 128 531 2.07e-120 367
MsaG010726 AT1G07520.3 46.782 404 186 6 237 611 293 696 2.68e-119 370
MsaG010726 AT1G07520.1 46.782 404 186 6 237 611 292 695 3.56e-119 369
MsaG010726 AT1G50600.3 30.273 403 254 9 230 608 126 525 7.52e-48 176
MsaG010726 AT1G50600.1 30.273 403 254 9 230 608 197 596 3.13e-47 175
MsaG010726 AT1G50600.2 30.273 403 254 9 230 608 201 600 4.12e-47 175
MsaG010726 AT5G48150.1 29.058 382 247 6 250 608 109 489 5.47e-47 172
MsaG010726 AT5G48150.4 29.058 382 247 6 250 608 109 489 5.47e-47 172
MsaG010726 AT5G48150.2 29.058 382 247 6 250 608 109 489 5.47e-47 172
MsaG010726 AT5G48150.3 29.058 382 247 6 250 608 109 489 5.47e-47 172
MsaG010726 AT2G04890.1 28.226 372 237 4 259 608 49 412 8.63e-42 156
MsaG010726 AT4G17230.1 28.533 375 245 4 258 610 153 526 4.04e-39 151
MsaG010726 AT4G17230.2 28.533 375 245 4 258 610 153 526 4.04e-39 151
MsaG010726 AT1G14920.1 27.791 421 251 12 229 608 120 528 4.36e-37 145
MsaG010726 AT3G03450.1 28.827 392 234 10 251 608 164 544 1.51e-36 144
MsaG010726 AT1G21450.1 25.543 368 252 5 263 608 225 592 1.25e-35 142
MsaG010726 AT1G66350.1 28.571 392 226 12 250 608 135 505 2.34e-35 140
MsaG010726 AT5G17490.1 29.443 377 226 11 257 608 154 515 4.79e-35 139
MsaG010726 AT3G54220.1 28.150 373 223 10 266 610 296 651 1.63e-34 139
MsaG010726 AT1G50420.1 24.362 431 238 7 260 608 54 478 5.20e-34 135
MsaG010726 AT2G01570.1 26.667 375 240 10 257 608 218 580 1.26e-33 136
MsaG010726 AT5G66770.1 27.078 373 242 8 260 608 217 583 2.55e-32 132
MsaG010726 AT5G41920.1 25.000 384 241 8 254 608 34 399 6.21e-28 116
MsaG010726 AT4G37650.1 24.069 403 235 12 262 610 145 530 1.58e-26 114
MsaG010726 AT3G50650.1 24.267 375 248 8 264 608 173 541 4.33e-26 113
MsaG010726 AT5G52510.1 29.680 219 149 5 392 608 424 639 6.27e-26 113
MsaG010726 AT1G63100.2 25.000 392 243 12 259 609 273 654 6.64e-24 107
MsaG010726 AT1G63100.1 25.000 392 243 12 259 609 248 629 8.23e-24 106
MsaG010726 AT3G49950.1 26.786 392 231 14 263 610 30 409 2.80e-22 100
MsaG010726 AT4G08250.1 25.967 362 226 13 279 608 125 476 4.44e-19 90.9
MsaG010726 AT1G55580.1 25.000 268 168 5 359 608 192 444 4.80e-17 84.3
MsaG010726 AT5G67411.1 29.648 199 123 9 360 545 4 198 3.67e-11 63.5

Find 128 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGACTACAAAATCTTTA+TGG 0.219672 2:-77090704 None:intergenic
CTTGGTCAGTGCTACCATTT+GGG 0.231237 2:-77089129 None:intergenic
TATGTATCTTAGGATGTTAT+TGG 0.255000 2:-77088984 None:intergenic
CCTCCAAAGCTTCGAATTAC+AGG 0.281535 2:+77090081 MsaT010726.1:CDS
CACTATTTCGAAAATCGTTT+TGG 0.292516 2:-77089964 None:intergenic
TCATCATCTTCACCTTCTTC+TGG 0.302876 2:+77088920 MsaT010726.1:CDS
AGGCAACACTCTTCGCCTTT+TGG 0.305061 2:+77089768 MsaT010726.1:CDS
ACTCCATGGAAGAAGACTTT+TGG 0.314241 2:-77090375 None:intergenic
GTTACTTGGTTTACCGAGTT+TGG 0.314473 2:-77088875 None:intergenic
TTCCTTCTTCACCATTGCTT+TGG 0.317965 2:-77090679 None:intergenic
TCTTGGTCAGTGCTACCATT+TGG 0.323016 2:-77089130 None:intergenic
GCAGGAATGTTTAAGACTTC+AGG 0.330542 2:+77089060 MsaT010726.1:CDS
TTTGTATGCTTTATCTGCTT+GGG 0.337273 2:+77090767 MsaT010726.1:CDS
ATAACTCATATGAATCTAAC+TGG 0.344405 2:+77089209 MsaT010726.1:CDS
AAGTCCTTCTATGATGTTCT+TGG 0.346747 2:+77089091 MsaT010726.1:CDS
TTAGCTCATTACTTTGCTAA+TGG 0.355828 2:+77089807 MsaT010726.1:CDS
CAAAATTATAAGATGCTTGT+TGG 0.356875 2:-77089587 None:intergenic
GGATGTCACTTATGTATCTT+AGG 0.358360 2:-77088994 None:intergenic
ATTCCTGTAATTCGAAGCTT+TGG 0.359837 2:-77090084 None:intergenic
AATCAGGTTGGATTCTGATT+TGG 0.361148 2:+77090647 MsaT010726.1:CDS
TAAAGGAGTTTGTAGTTGTA+TGG 0.363980 2:-77089263 None:intergenic
ACTCAAACCCATTTGTTACT+TGG 0.366037 2:-77088889 None:intergenic
AGCTTCAACGCAGCTTCTCT+TGG 0.373655 2:-77090336 None:intergenic
GCAGAAAGGGTGGAGGAAAC+TGG 0.374425 2:+77090135 MsaT010726.1:CDS
AAATCTTGCAGAAAGGTGTT+TGG 0.378133 2:-77088845 None:intergenic
CCTCTTGGTTTGGCAACTGC+TGG 0.385884 2:+77089870 MsaT010726.1:CDS
CACGATCGAAATTTCCATCT+TGG 0.387639 2:-77089147 None:intergenic
ATCGCTGTTTGATATGTTTG+AGG 0.388834 2:+77090464 MsaT010726.1:CDS
ATCATATCAGCCTCTTGGTT+TGG 0.393034 2:+77089860 MsaT010726.1:CDS
GTTTCCTCCACCCTTTCTGC+AGG 0.394328 2:-77090132 None:intergenic
AAGCTGCGTTGAAGCTGATC+AGG 0.403550 2:+77090343 MsaT010726.1:CDS
CCTCGTGTCTCGATGACAAC+TGG 0.405616 2:-77089322 None:intergenic
TCTTCTGGCACAAGCTCAAA+TGG 0.409923 2:+77088935 MsaT010726.1:CDS
ACCATTGTCACAACGGAACT+TGG 0.411323 2:+77089350 MsaT010726.1:CDS
GAGCTGGGTTCAAGCAAATC+AGG 0.413441 2:+77090631 MsaT010726.1:CDS
TCGGTAAACCAAGTAACAAA+TGG 0.413464 2:+77088881 MsaT010726.1:CDS
CGTACAAGCAGTGGCAGGTC+AGG 0.432935 2:+77090601 MsaT010726.1:CDS
ATGCTTGAGAGAGGGTTGTT+TGG 0.436357 2:+77090519 MsaT010726.1:CDS
TTCAATGCCACAGTTTCATC+AGG 0.440053 2:-77090309 None:intergenic
AAGCTCAAATGGAGAATCCT+TGG 0.456686 2:+77088946 MsaT010726.1:CDS
TGCCACTGCTTGTACGTCTC+AGG 0.458186 2:-77090594 None:intergenic
CATTTGGGGATGGATGGTTA+TGG 0.459747 2:-77089114 None:intergenic
CATGGATGAAGAAGATGACT+TGG 0.465816 2:+77089018 MsaT010726.1:CDS
TTGAGAGAGGGTTGTTTGGA+AGG 0.472118 2:+77090523 MsaT010726.1:CDS
GAAACTTCTTCCACCGTCAC+TGG 0.475504 2:-77089391 None:intergenic
ATGTTGCGGCTGTTGATTTG+TGG 0.475907 2:+77089670 MsaT010726.1:CDS
TGCTACCATTTGGGGATGGA+TGG 0.476955 2:-77089120 None:intergenic
GTGCTTCCTTAAACCGAGTC+AGG 0.477530 2:-77090428 None:intergenic
TTTGAGCTTGTGCCAGAAGA+AGG 0.477829 2:-77088932 None:intergenic
AGCACCTCATCTAAAATCTC+TGG 0.485983 2:-77089531 None:intergenic
AGCTTGTGAGGGAGCAGAAA+GGG 0.488617 2:+77090563 MsaT010726.1:CDS
CTTTCTGCAGGTCTAAATCC+TGG 0.490039 2:-77090120 None:intergenic
CGGTAAACCAAGTAACAAAT+GGG 0.492040 2:+77088882 MsaT010726.1:CDS
TAGTCGATGTGGATGGTAAG+TGG 0.492425 2:+77090718 MsaT010726.1:CDS
TGAGAGAGGGTTGTTTGGAA+GGG 0.496075 2:+77090524 MsaT010726.1:CDS
CGAAGCTTTGGAGGACCACC+AGG 0.497005 2:-77090072 None:intergenic
CCTGTAATTCGAAGCTTTGG+AGG 0.497546 2:-77090081 None:intergenic
AACCAAAGCAATGGTGAAGA+AGG 0.500696 2:+77090677 MsaT010726.1:CDS
ATGGCCAAGAACATCATAGA+AGG 0.501527 2:-77089095 None:intergenic
AATCCAAAAGTCTTCTTCCA+TGG 0.502095 2:+77090372 MsaT010726.1:CDS
TAATGGTCTTGAGATAAGAT+TGG 0.503318 2:+77089824 MsaT010726.1:CDS
GGAATTGAGCTTCCTCAGCC+AGG 0.503697 2:+77090102 MsaT010726.1:CDS
CAGGTCAGGAACAAGAGAGC+TGG 0.506156 2:+77090615 MsaT010726.1:CDS
CTTGAGATCCTTGAGACGGA+TGG 0.508334 2:-77090232 None:intergenic
TTCTTCCATGGAGTTGCCAA+TGG 0.510258 2:+77090384 MsaT010726.1:CDS
ATGAGCTACTCAAACAGATT+AGG 0.511922 2:+77089748 MsaT010726.1:CDS
AGGATTTAGACCTGCAGAAA+GGG 0.512549 2:+77090122 MsaT010726.1:CDS
GGAATATCTTGTCATTTCCA+AGG 0.512718 2:-77088963 None:intergenic
TCAAGGATCTCAAGATAGAC+AGG 0.512857 2:+77090241 MsaT010726.1:CDS
AAACCAAGAGGCTGATATGA+TGG 0.515320 2:-77089858 None:intergenic
TATAAATGTTATAGCTTGTG+AGG 0.517961 2:+77090551 MsaT010726.1:CDS
CAACTTCATTGCACAGAGAT+GGG 0.518376 2:+77090206 MsaT010726.1:CDS
ACGCCATCATATCAGCCTCT+TGG 0.518385 2:+77089855 MsaT010726.1:CDS
ACAACTTCATTGCACAGAGA+TGG 0.523539 2:+77090205 MsaT010726.1:CDS
CGTTGGAGTCCATTGCCAAA+AGG 0.523700 2:-77089783 None:intergenic
CTGTGGCGAGCTACGATCAA+AGG 0.536487 2:+77089718 MsaT010726.1:CDS
TCCAAGTTCCGTTGTGACAA+TGG 0.537164 2:-77089351 None:intergenic
AACTTCCATTGGCAACTCCA+TGG 0.540581 2:-77090389 None:intergenic
CATAACCATCCATCCCCAAA+TGG 0.544430 2:+77089115 MsaT010726.1:CDS
GTCACAACGGAACTTGGAGT+TGG 0.544454 2:+77089356 MsaT010726.1:CDS
CAGGATTTAGACCTGCAGAA+AGG 0.549603 2:+77090121 MsaT010726.1:CDS
GCAATGAAGTTGTATTCAAA+TGG 0.550757 2:-77090195 None:intergenic
AGTCGATGTGGATGGTAAGT+GGG 0.556259 2:+77090719 MsaT010726.1:CDS
CGGTGCGCTATAACTTCCAT+TGG 0.556645 2:-77090400 None:intergenic
AACACTAAAATCTTGCAGAA+AGG 0.557457 2:-77088852 None:intergenic
TTGTGCACATTGATTTAGCA+TGG 0.558170 2:-77089694 None:intergenic
GCATTTGTCATTCTCTGCAA+AGG 0.559014 2:-77089933 None:intergenic
AAACATTCCTGCAACATGCA+TGG 0.559801 2:-77089049 None:intergenic
ATGCCACAGTTTCATCAGGA+AGG 0.560068 2:-77090305 None:intergenic
AATGGTAGCACTGACCAAGA+TGG 0.569750 2:+77089133 MsaT010726.1:CDS
CAAAGTAATGAGCTAAACGT+TGG 0.573182 2:-77089800 None:intergenic
TTGGTCAGTGCTACCATTTG+GGG 0.574197 2:-77089128 None:intergenic
AGGTCAGGAACAAGAGAGCT+GGG 0.576170 2:+77090616 MsaT010726.1:CDS
ATACATAAGTGACATCCTCA+TGG 0.582153 2:+77089000 MsaT010726.1:CDS
ATAAATGTTATAGCTTGTGA+GGG 0.582885 2:+77090552 MsaT010726.1:CDS
ATGGGAAACCATCCGTCTCA+AGG 0.585121 2:+77090224 MsaT010726.1:CDS
AGGGAGCAGAAAGGGTTGAA+AGG 0.587349 2:+77090571 MsaT010726.1:CDS
GAGGTGCTGCTTTATCAGAC+AGG 0.590907 2:+77089546 MsaT010726.1:CDS
TGAGACGTACAAGCAGTGGC+AGG 0.594436 2:+77090596 MsaT010726.1:CDS
CAGAGGTCGATGCTTGAGAG+AGG 0.600053 2:+77090510 MsaT010726.1:CDS
TGATTCAGGAGGAAAGGCAA+AGG 0.600358 2:+77089608 MsaT010726.1:CDS
ATTTAGACCTGCAGAAAGGG+TGG 0.601556 2:+77090125 MsaT010726.1:CDS
TCAGTGCTACCATTTGGGGA+TGG 0.608274 2:-77089124 None:intergenic
ACCTCATCTAAAATCTCTGG+TGG 0.612420 2:-77089528 None:intergenic
TGGGTTCAAGCAAATCAGGT+TGG 0.613823 2:+77090635 MsaT010726.1:CDS
GCAGGTCTAAATCCTGGCTG+AGG 0.615245 2:-77090114 None:intergenic
GGCCTGAGACGTACAAGCAG+TGG 0.615272 2:+77090592 MsaT010726.1:CDS
AAGAGTCGAATTCGACGACA+TGG 0.616744 2:-77089237 None:intergenic
TAGACCTGCAGAAAGGGTGG+AGG 0.621359 2:+77090128 MsaT010726.1:CDS
TAGCTTGTGAGGGAGCAGAA+AGG 0.625836 2:+77090562 MsaT010726.1:CDS
CTATCTTGAGATCCTTGAGA+CGG 0.633125 2:-77090236 None:intergenic
TGACATTCAGCCAGTGACGG+TGG 0.640113 2:+77089381 MsaT010726.1:CDS
CCAGCAGTTGCCAAACCAAG+AGG 0.642152 2:-77089870 None:intergenic
GACCTCTGCGTGTCTTCACG+AGG 0.643181 2:-77090495 None:intergenic
AGAAAACTTTCATTAAGACC+TGG 0.645902 2:+77090054 MsaT010726.1:CDS
GAATGACATTCAGCCAGTGA+CGG 0.650343 2:+77089378 MsaT010726.1:CDS
AGAGGTCGATGCTTGAGAGA+GGG 0.653291 2:+77090511 MsaT010726.1:CDS
CCAGTTGTCATCGAGACACG+AGG 0.656087 2:+77089322 MsaT010726.1:CDS
GGTGAATGAAACCAAAGCAA+TGG 0.658724 2:+77090668 MsaT010726.1:CDS
GAACCTTCCTGATGAAACTG+TGG 0.660816 2:+77090302 MsaT010726.1:CDS
AAATCAATGTGCACAAGCTG+TGG 0.661819 2:+77089701 MsaT010726.1:CDS
AAAGGGTGGAGGAAACTGGA+CGG 0.662270 2:+77090139 MsaT010726.1:CDS
AAACTTTCATTAAGACCTGG+TGG 0.664266 2:+77090057 MsaT010726.1:CDS
AGATTCATATGAGTTATCAG+TGG 0.666994 2:-77089203 None:intergenic
TAACTCATATGAATCTAACT+GGG 0.675488 2:+77089210 MsaT010726.1:CDS
AGTCATCTTCTTCATCCATG+AGG 0.688746 2:-77089015 None:intergenic
GGTGCTAACCATTGTCACAA+CGG 0.691376 2:+77089343 MsaT010726.1:CDS
TGCCTCGTGAAGACACGCAG+AGG 0.691967 2:+77090493 MsaT010726.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 77088836 77090800 77088836 ID=MsaG010726
Chr2 mRNA 77088836 77090800 77088836 ID=MsaT010726.1;Parent=MsaG010726
Chr2 exon 77088836 77089423 77088836 ID=MsaT010726.1.exon1;Parent=MsaT010726.1
Chr2 CDS 77088836 77089423 77088836 ID=cds.MsaT010726.1;Parent=MsaT010726.1
Chr2 exon 77089498 77089983 77089498 ID=MsaT010726.1.exon2;Parent=MsaT010726.1
Chr2 CDS 77089498 77089983 77089498 ID=cds.MsaT010726.1;Parent=MsaT010726.1
Chr2 exon 77090039 77090800 77090039 ID=MsaT010726.1.exon3;Parent=MsaT010726.1
Chr2 CDS 77090039 77090800 77090039 ID=cds.MsaT010726.1;Parent=MsaT010726.1
Gene Sequence

>MsaT010726.1

ATGCTTTCTCCAAACACCTTTCTGCAAGATTTTAGTGTTCCAAACTCGGTAAACCAAGTAACAAATGGGTTTGAGTTAGAAGATTCATCATCTTCACCTTCTTCTGGCACAAGCTCAAATGGAGAATCCTTGGAAATGACAAGATATTCCAATAACATCCTAAGATACATAAGTGACATCCTCATGGATGAAGAAGATGACTTGGAGCAAAAACCATGCATGTTGCAGGAATGTTTAAGACTTCAGGCTGCTGAAAAGTCCTTCTATGATGTTCTTGGCCATAACCATCCATCCCCAAATGGTAGCACTGACCAAGATGGAAATTTCGATCGTGCTGCTAGTTTTGAGAGAAATGGCAGCAGCTGCACCACTGATAACTCATATGAATCTAACTGGGTCAACCATGTCGTCGAATTCGACTCTTCTTCCATACAACTACAAACTCCTTTAATAGAAAAAAACTACTATGACTTGACAGAAGCTGATCCAGTTGTCATCGAGACACGAGGTGCTAACCATTGTCACAACGGAACTTGGAGTTGGAATGACATTCAGCCAGTGACGGTGGAAGAAGTTTCACCTTCAACAGTTTCAGCTATTTTCTCTGATGAATTAGAGCCACCAGAGATTTTAGATGAGGTGCTGCTTTATCAGACAGGAAAGACTCTCACTCAACACCAACAAGCATCTTATAATTTTGATTCAGGAGGAAAGGCAAAGGAAACACATTCGCGTTTGAAGAAAGCATCTACTACGAAAAATGTTGCGGCTGTTGATTTGTGGACCATGCTAAATCAATGTGCACAAGCTGTGGCGAGCTACGATCAAAGGAATACAGATGAGCTACTCAAACAGATTAGGCAACACTCTTCGCCTTTTGGCAATGGACTCCAACGTTTAGCTCATTACTTTGCTAATGGTCTTGAGATAAGATTGGCTGCTGAGACGCCATCATATCAGCCTCTTGGTTTGGCAACTGCTGGAGATATGTTGAAAGCTTACAAACTATTTGTTACTGCGTCTCCTTTGCAGAGAATGACAAATGCTTTGCTAACCAAAACGATTTTCGAAATATGGCCGTGCCTCGTCAGAAAACTTTCATTAAGACCTGGTGGTCCTCCAAAGCTTCGAATTACAGGAATTGAGCTTCCTCAGCCAGGATTTAGACCTGCAGAAAGGGTGGAGGAAACTGGACGGCGTCTAGCAAACTACTGCAAGAAGTTCAATGTACCATTTGAATACAACTTCATTGCACAGAGATGGGAAACCATCCGTCTCAAGGATCTCAAGATAGACAGGAATGAAATAACTCTTGTTAGCTGTTTGTACAGACTGAAGAACCTTCCTGATGAAACTGTGGCATTGAATTGTCCAAGAGAAGCTGCGTTGAAGCTGATCAGGAAGATTAATCCAAAAGTCTTCTTCCATGGAGTTGCCAATGGAAGTTATAGCGCACCGTTTTTCCTGACTCGGTTTAAGGAAGCACTCTATCACTTCTCATCGCTGTTTGATATGTTTGAGGCTAACGTGCCTCGTGAAGACACGCAGAGGTCGATGCTTGAGAGAGGGTTGTTTGGAAGGGATTCTATAAATGTTATAGCTTGTGAGGGAGCAGAAAGGGTTGAAAGGCCTGAGACGTACAAGCAGTGGCAGGTCAGGAACAAGAGAGCTGGGTTCAAGCAAATCAGGTTGGATTCTGATTTGGTGAATGAAACCAAAGCAATGGTGAAGAAGGAATACCATAAAGATTTTGTAGTCGATGTGGATGGTAAGTGGGTTTTAAAGGGATGGAAAGGACGCATTTTGTATGCTTTATCTGCTTGGGTTCCTGCTTAA
Protein sequence

>MsaT010726.1

MLSPNTFLQDFSVPNSVNQVTNGFELEDSSSSPSSGTSSNGESLEMTRYSNNILRYISDILMDEEDDLEQKPCMLQECLRLQAAEKSFYDVLGHNHPSPNGSTDQDGNFDRAASFERNGSSCTTDNSYESNWVNHVVEFDSSSIQLQTPLIEKNYYDLTEADPVVIETRGANHCHNGTWSWNDIQPVTVEEVSPSTVSAIFSDELEPPEILDEVLLYQTGKTLTQHQQASYNFDSGGKAKETHSRLKKASTTKNVAAVDLWTMLNQCAQAVASYDQRNTDELLKQIRQHSSPFGNGLQRLAHYFANGLEIRLAAETPSYQPLGLATAGDMLKAYKLFVTASPLQRMTNALLTKTIFEIWPCLVRKLSLRPGGPPKLRITGIELPQPGFRPAERVEETGRRLANYCKKFNVPFEYNFIAQRWETIRLKDLKIDRNEITLVSCLYRLKNLPDETVALNCPREAALKLIRKINPKVFFHGVANGSYSAPFFLTRFKEALYHFSSLFDMFEANVPREDTQRSMLERGLFGRDSINVIACEGAERVERPETYKQWQVRNKRAGFKQIRLDSDLVNETKAMVKKEYHKDFVVDVDGKWVLKGWKGRILYALSAWVPA*