AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010727

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010727 MtrunA17_Chr2g0328431 94.360 656 34 3 1 653 1 656 0.0 1256
MsaG010727 MtrunA17_Chr2g0328411 90.214 654 61 3 1 651 1 654 0.0 1188
MsaG010727 MtrunA17_Chr2g0328401 80.031 636 123 4 1 632 1 636 0.0 1030
MsaG010727 MtrunA17_Chr4g0031741 53.623 690 245 12 1 653 1 652 0.0 671
MsaG010727 MtrunA17_Chr7g0238891 43.129 684 309 13 43 653 66 742 8.64e-180 528
MsaG010727 MtrunA17_Chr2g0328391 42.986 663 348 14 4 653 26 671 8.93e-170 500
MsaG010727 MtrunA17_Chr4g0031701 45.182 633 307 15 37 653 20 628 2.16e-166 490
MsaG010727 MtrunA17_Chr4g0031721 53.092 469 200 7 198 653 225 686 1.61e-165 490
MsaG010727 MtrunA17_Chr4g0031711 43.070 671 314 18 37 653 79 735 1.97e-163 486
MsaG010727 MtrunA17_Chr4g0031731 44.866 633 309 15 37 653 20 628 1.10e-162 481
MsaG010727 MtrunA17_Chr2g0328381 45.958 433 220 6 227 653 219 643 6.72e-131 399
MsaG010727 MtrunA17_Chr2g0328341 46.420 433 217 7 227 653 217 640 1.18e-129 396
MsaG010727 MtrunA17_Chr2g0328361 46.296 378 170 6 281 653 268 617 2.77e-112 350
MsaG010727 MtrunA17_Chr2g0328371 51.172 256 120 2 354 608 1 252 8.17e-92 285
MsaG010727 MtrunA17_Chr2g0290241 29.491 373 259 3 282 650 52 424 3.25e-59 204
MsaG010727 MtrunA17_Chr2g0319091 32.225 391 258 6 265 650 190 578 1.74e-56 201
MsaG010727 MtrunA17_Chr5g0447691 32.440 373 245 6 283 650 173 543 2.73e-56 200
MsaG010727 MtrunA17_Chr4g0076091 29.340 409 272 7 257 651 138 543 2.03e-55 197
MsaG010727 MtrunA17_Chr1g0159461 28.338 367 260 2 287 650 225 591 6.46e-51 186
MsaG010727 MtrunA17_Chr8g0376381 31.733 375 235 9 281 650 163 521 3.54e-50 182
MsaG010727 MtrunA17_Chr3g0101141 29.111 371 260 2 283 650 171 541 4.03e-50 182
MsaG010727 MtrunA17_Chr7g0224271 30.479 397 233 9 280 650 293 672 1.34e-49 184
MsaG010727 MtrunA17_Chr5g0445331 29.973 377 253 7 283 650 157 531 1.64e-49 181
MsaG010727 MtrunA17_Chr3g0125811 27.793 367 262 2 287 650 203 569 4.79e-48 177
MsaG010727 MtrunA17_Chr4g0057891 30.179 391 242 9 279 650 141 519 7.25e-48 176
MsaG010727 MtrunA17_Chr8g0357601 29.923 391 244 7 281 653 176 554 4.69e-47 174
MsaG010727 MtrunA17_Chr2g0306641 29.825 399 251 12 267 650 102 486 2.19e-46 171
MsaG010727 MtrunA17_Chr3g0110971 28.831 385 248 8 281 650 213 586 3.67e-44 167
MsaG010727 MtrunA17_Chr7g0245601 30.245 367 238 8 290 652 441 793 1.82e-42 164
MsaG010727 MtrunA17_Chr4g0038851 26.464 427 245 7 287 650 48 468 1.17e-38 149
MsaG010727 MtrunA17_Chr2g0294461 28.021 389 251 7 282 650 205 584 1.42e-38 150
MsaG010727 MtrunA17_Chr3g0085681 26.923 416 245 6 288 650 26 435 2.03e-38 147
MsaG010727 MtrunA17_Chr4g0038761 28.912 377 236 10 290 653 87 444 2.61e-37 144
MsaG010727 MtrunA17_Chr4g0057291 23.711 388 270 10 281 652 286 663 7.15e-33 134
MsaG010727 MtrunA17_Chr4g0039661 25.729 377 258 7 284 650 190 554 6.09e-32 130
MsaG010727 MtrunA17_Chr2g0294471 26.234 385 260 8 282 650 199 575 1.72e-31 129
MsaG010727 MtrunA17_Chr1g0200401 26.010 396 247 12 287 650 77 458 6.98e-30 123
MsaG010727 MtrunA17_Chr5g0401131 25.521 384 262 9 287 652 114 491 1.90e-29 122
MsaG010727 MtrunA17_Chr1g0183641 27.297 381 245 13 281 650 117 476 7.78e-29 120
MsaG010727 MtrunA17_Chr7g0270031 26.121 379 261 8 282 650 184 553 8.18e-29 121
MsaG010727 MtrunA17_Chr7g0236851 29.119 261 178 4 391 650 402 656 2.82e-27 117
MsaG010727 MtrunA17_Chr5g0424131 26.667 360 231 12 314 651 151 499 9.33e-27 114
MsaG010727 MtrunA17_Chr3g0114841 28.846 312 194 11 353 651 204 500 8.12e-26 111
MsaG010727 MtrunA17_Chr8g0383981 24.339 378 247 15 292 653 144 498 1.27e-25 111
MsaG010727 MtrunA17_Chr6g0468561 25.789 380 251 11 288 650 296 661 1.84e-24 108
MsaG010727 MtrunA17_Chr3g0085361 25.429 350 205 5 350 650 7 349 2.67e-24 104
MsaG010727 MtrunA17_Chr5g0401421 22.300 426 278 9 277 653 27 448 3.85e-24 105
MsaG010727 MtrunA17_Chr5g0396491 32.057 209 132 2 287 491 56 258 4.42e-24 106
MsaG010727 MtrunA17_Chr5g0396491 30.872 149 102 1 503 650 329 477 2.04e-17 85.5
MsaG010727 MtrunA17_Chr1g0194621 23.143 350 241 8 318 650 179 517 3.06e-22 100
MsaG010727 MtrunA17_Chr4g0053901 22.169 415 272 7 287 653 59 470 3.55e-22 100
MsaG010727 MtrunA17_Chr2g0329591 26.700 397 242 13 287 652 74 452 4.10e-21 96.7
MsaG010727 MtrunA17_Chr8g0344101 24.750 400 242 16 287 650 223 599 8.51e-16 80.9
MsaG010727 MtrunA17_Chr7g0230731 23.592 373 257 11 288 650 373 727 1.19e-14 77.8
MsaG010727 MtrunA17_Chr7g0230771 22.911 371 262 10 288 650 376 730 3.25e-14 76.3
MsaG010727 MtrunA17_Chr2g0323111 25.843 178 130 2 477 653 132 308 1.15e-13 72.4
MsaG010727 MtrunA17_Chr4g0012071 23.711 194 138 3 273 460 178 367 6.23e-13 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010727 AT2G29060.2 43.265 631 301 13 49 652 56 656 8.50e-166 490
MsaG010727 AT2G37650.1 43.101 645 319 15 49 652 77 714 3.42e-162 483
MsaG010727 AT2G37650.2 43.101 645 319 15 49 652 65 702 3.50e-162 482
MsaG010727 AT2G29060.1 42.434 641 329 12 49 652 56 693 4.25e-162 482
MsaG010727 AT5G59450.1 50.753 465 212 8 202 653 140 600 1.44e-155 462
MsaG010727 AT1G07530.1 49.367 474 224 6 193 653 297 767 4.77e-155 466
MsaG010727 AT3G46600.3 51.556 450 192 7 214 653 116 549 1.05e-148 442
MsaG010727 AT3G46600.1 51.225 449 195 6 214 653 148 581 1.28e-148 443
MsaG010727 AT3G46600.2 56.216 370 159 2 287 653 4 373 2.81e-146 429
MsaG010727 AT2G29065.1 41.311 656 326 16 22 653 10 630 3.04e-144 434
MsaG010727 AT1G07520.2 44.398 482 249 6 183 653 58 531 7.72e-134 404
MsaG010727 AT1G07520.4 44.398 482 249 6 183 653 58 531 7.72e-134 404
MsaG010727 AT1G07520.3 44.398 482 249 6 183 653 223 696 2.39e-132 405
MsaG010727 AT1G07520.1 44.398 482 249 6 183 653 222 695 2.94e-132 405
MsaG010727 AT1G50600.3 33.423 371 244 2 283 650 155 525 7.20e-60 209
MsaG010727 AT1G50600.1 33.423 371 244 2 283 650 226 596 4.06e-59 209
MsaG010727 AT1G50600.2 33.423 371 244 2 283 650 230 600 4.55e-59 209
MsaG010727 AT5G48150.1 30.890 382 258 4 274 650 109 489 5.54e-55 195
MsaG010727 AT5G48150.4 30.890 382 258 4 274 650 109 489 5.54e-55 195
MsaG010727 AT5G48150.2 30.890 382 258 4 274 650 109 489 5.54e-55 195
MsaG010727 AT5G48150.3 30.890 382 258 4 274 650 109 489 5.54e-55 195
MsaG010727 AT2G04890.1 29.650 371 251 3 283 650 49 412 7.21e-51 182
MsaG010727 AT4G17230.1 30.214 374 258 2 282 652 153 526 4.50e-50 182
MsaG010727 AT4G17230.2 30.214 374 258 2 282 652 153 526 4.50e-50 182
MsaG010727 AT1G14920.1 31.200 375 241 9 281 650 166 528 7.97e-48 176
MsaG010727 AT3G03450.1 29.820 389 233 9 281 650 177 544 5.88e-46 171
MsaG010727 AT2G01570.1 29.067 375 249 9 281 650 218 580 5.72e-44 166
MsaG010727 AT1G21450.1 27.717 368 262 3 287 650 225 592 1.18e-43 166
MsaG010727 AT3G54220.1 30.645 372 233 8 290 652 296 651 1.72e-43 166
MsaG010727 AT1G66350.1 30.423 378 234 11 281 650 149 505 8.21e-43 162
MsaG010727 AT5G17490.1 29.630 378 242 9 281 650 154 515 1.04e-41 159
MsaG010727 AT5G66770.1 28.686 373 254 7 284 650 217 583 2.65e-41 159
MsaG010727 AT1G50420.1 25.058 431 253 7 284 650 54 478 1.13e-38 149
MsaG010727 AT3G50650.1 26.667 375 257 7 288 650 173 541 4.66e-37 146
MsaG010727 AT5G41920.1 27.979 386 247 9 277 650 33 399 5.84e-37 143
MsaG010727 AT4G37650.1 25.062 403 249 11 286 652 145 530 1.78e-33 135
MsaG010727 AT1G63100.1 26.062 353 240 7 283 625 248 589 2.20e-31 130
MsaG010727 AT1G63100.2 26.062 353 240 7 283 625 273 614 2.39e-31 130
MsaG010727 AT5G52510.1 26.525 377 253 9 290 650 271 639 2.74e-31 130
MsaG010727 AT4G08250.1 28.571 343 222 10 321 650 144 476 1.98e-29 122
MsaG010727 AT3G49950.1 27.273 385 256 10 287 652 30 409 2.46e-29 121
MsaG010727 AT1G55580.1 24.156 385 225 9 318 650 75 444 2.23e-23 103
MsaG010727 AT3G13840.1 24.093 386 247 13 287 650 148 509 4.01e-19 91.7
MsaG010727 AT5G67411.1 30.198 202 124 9 400 588 1 198 6.34e-11 62.8

Find 142 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCACAAGCTATAACATTTA+TGG 0.192701 2:-77093564 None:intergenic
CATCGACTACAAAATCTTTA+TGG 0.219672 2:-77093715 None:intergenic
AGGCAACACTCTTTGTCTTT+TGG 0.250282 2:+77092780 MsaT010727.1:CDS
TATGTATCTTAGGATGTTAT+TGG 0.255000 2:-77091998 None:intergenic
AGCGTTCATATTATTGATTT+TGG 0.268671 2:+77093011 MsaT010727.1:CDS
CCTGACCTGCCACTGCTTGT+AGG 0.274499 2:-77093615 None:intergenic
CCTCCAAAGCTTCGAATTAC+AGG 0.281535 2:+77093095 MsaT010727.1:CDS
GTTACTTGGTTTACTGAGTT+TGG 0.288731 2:-77091889 None:intergenic
ACTCCATGGAAGAATACTTT+TGG 0.310176 2:-77093389 None:intergenic
TCACCATCTTCACCTTCTTC+TGG 0.322046 2:+77091934 MsaT010727.1:CDS
GCAGGAATGTTTAAGACTTC+AGG 0.330542 2:+77092074 MsaT010727.1:CDS
CGCGATCGAAATTTCCATTT+GGG 0.332531 2:-77092161 None:intergenic
AAGTCCTTCTATGATGTTCT+TGG 0.346747 2:+77092105 MsaT010727.1:CDS
ACAACTTCATTGCACAGAAA+TGG 0.347339 2:+77093219 MsaT010727.1:CDS
TTTGAATGCTTTATCAGCTT+GGG 0.351490 2:+77093778 MsaT010727.1:CDS
GGATGTCACTTATGTATCTT+AGG 0.358360 2:-77092008 None:intergenic
ATTCCTGTAATTCGAAGCTT+TGG 0.359837 2:-77093098 None:intergenic
GCGCGATCGAAATTTCCATT+TGG 0.365718 2:-77092162 None:intergenic
GCATCACATAATGTTGATTC+AGG 0.366614 2:+77092606 MsaT010727.1:CDS
TTAGCTCATTACTTTGCTAA+TGG 0.370579 2:+77092819 MsaT010727.1:CDS
TAAAGGACTTTGTAGTTGTA+TGG 0.371367 2:-77092277 None:intergenic
AAATCTTGCAGAAAGGTGTT+TGG 0.378133 2:-77091859 None:intergenic
CATTTGGTGAAGGCTGGTTA+TGG 0.381462 2:-77092128 None:intergenic
TTTGGGTCAGTGCTATCATT+TGG 0.388999 2:-77092144 None:intergenic
AGCTTCAACACAGCTTCTCT+TGG 0.390505 2:-77093350 None:intergenic
CCTCTTGATGTGGCAACTGC+TGG 0.394427 2:+77092882 MsaT010727.1:CDS
TTCTGACGAGGCACGGCCAC+TGG 0.399566 2:-77093049 None:intergenic
TCAGTAAACCAAGTAACAAA+TGG 0.401437 2:+77091895 MsaT010727.1:CDS
ATGCTTGAGAGAGGGTTGTT+TGG 0.402972 2:+77093533 MsaT010727.1:CDS
CCTTGTGTCTCGATGACAAC+TGG 0.405616 2:-77092333 None:intergenic
ATTCAAACCCATTTGTTACT+TGG 0.405951 2:-77091903 None:intergenic
CCTACAAGCAGTGGCAGGTC+AGG 0.408410 2:+77093615 MsaT010727.1:CDS
TCTTCTGGCACAAGCTCAAA+TGG 0.409923 2:+77091949 MsaT010727.1:CDS
GAGCTGGGTTCAAGCAAATC+AGG 0.413441 2:+77093645 MsaT010727.1:CDS
GTTTCCTCCACCCTTTCTGC+AGG 0.420825 2:-77093146 None:intergenic
GCAAAATCCCTGTATGTTGC+AGG 0.423000 2:+77092056 MsaT010727.1:CDS
CAACATTATGTGATGCTTGT+TGG 0.426130 2:-77092599 None:intergenic
CAACTTCATTGCACAGAAAT+GGG 0.427023 2:+77093220 MsaT010727.1:CDS
TGCCACTGCTTGTAGGTCTC+AGG 0.429715 2:-77093608 None:intergenic
ACCATTGTCATAACGGAACT+TGG 0.436424 2:+77092361 MsaT010727.1:CDS
ATCGTTGTTTGATATGTTTG+AGG 0.437931 2:+77093478 MsaT010727.1:CDS
ATGTTATTGGAATATCTTGT+CGG 0.439317 2:-77091985 None:intergenic
TTCAATGCCACAGTTTCATC+AGG 0.440053 2:-77093323 None:intergenic
TAAACATTCCTGCAACATAC+AGG 0.459008 2:-77092064 None:intergenic
TGCTATCATTTGGTGAAGGC+TGG 0.460261 2:-77092134 None:intergenic
AAGCTCAAATGGAGAATCCT+TGG 0.463718 2:+77091960 MsaT010727.1:CDS
CATGGATGAAGAAGATGACT+TGG 0.465816 2:+77092032 MsaT010727.1:CDS
GCAGAAAGGGTGGAGGAAAC+CGG 0.469253 2:+77093149 MsaT010727.1:CDS
AAGCTGTGTTGAAGCTGATC+AGG 0.474231 2:+77093357 MsaT010727.1:CDS
TACAAACAATGCCGCGGCTG+TGG 0.476659 2:+77092674 MsaT010727.1:CDS
GTGCTTCCTTAAACCGAGTC+AGG 0.477530 2:-77093442 None:intergenic
TTTGAGCTTGTGCCAGAAGA+AGG 0.477829 2:-77091946 None:intergenic
GTCATAACGGAACTTGGAGT+TGG 0.479661 2:+77092367 MsaT010727.1:CDS
AATGGCAGCAGCTGCATCGC+TGG 0.480099 2:+77092201 MsaT010727.1:CDS
AGTACCTCATCTAAAATCTC+TGG 0.483079 2:-77092543 None:intergenic
AGCTTGTGAGGGTGCAGAAA+GGG 0.483774 2:+77093577 MsaT010727.1:CDS
TAGTCGATGTGGATGGTAAG+TGG 0.492425 2:+77093729 MsaT010727.1:CDS
ATGCCGCGGCTGTGGATTTG+TGG 0.495407 2:+77092682 MsaT010727.1:CDS
GGAATTGAGCTTCCTCGGCC+AGG 0.495711 2:+77093116 MsaT010727.1:CDS
CGAAGCTTTGGAGGACCACC+AGG 0.497005 2:-77093086 None:intergenic
CCTGTAATTCGAAGCTTTGG+AGG 0.497546 2:-77093095 None:intergenic
AATGATAGCACTGACCCAAA+TGG 0.500557 2:+77092147 MsaT010727.1:CDS
TGGAGGATCTAAAGATAGAC+AGG 0.500668 2:+77093255 MsaT010727.1:CDS
ATGGCCAAGAACATCATAGA+AGG 0.501527 2:-77092109 None:intergenic
TAATGGTCTTGAGATAAGAT+TGG 0.503318 2:+77092836 MsaT010727.1:CDS
CTTTCTGCAGGTCTAAATCC+TGG 0.504296 2:-77093134 None:intergenic
ATGAGCTACTCAAACAGATT+AGG 0.511922 2:+77092760 MsaT010727.1:CDS
CAGTAAACCAAGTAACAAAT+GGG 0.514266 2:+77091896 MsaT010727.1:CDS
TTACAGGAATTGAGCTTCCT+CGG 0.515630 2:+77093111 MsaT010727.1:CDS
TGTCTATCTTTAGATCCTCC+AGG 0.515870 2:-77093253 None:intergenic
CAGGTCAGGAATAAGAGAGC+TGG 0.517229 2:+77093629 MsaT010727.1:CDS
TTCTTCCATGGAGTTGCCAA+TGG 0.517479 2:+77093398 MsaT010727.1:CDS
AAAACTTCTTCCACCATCAC+TGG 0.518831 2:-77092402 None:intergenic
AGGATTTAGACCTGCAGAAA+GGG 0.522516 2:+77093136 MsaT010727.1:CDS
CTTTAGATCCTCCAGGCAGA+TGG 0.523098 2:-77093246 None:intergenic
AATCCAAAAGTATTCTTCCA+TGG 0.523808 2:+77093386 MsaT010727.1:CDS
ACATCAAGAGGCTGATATGA+TGG 0.524913 2:-77092870 None:intergenic
GGAATATCTTGTCGGTTCCA+AGG 0.526658 2:-77091977 None:intergenic
CTGTGGCGAGCTACGATCAA+AGG 0.536487 2:+77092730 MsaT010727.1:CDS
GAAATGGGAAACCATCTGCC+TGG 0.536630 2:+77093235 MsaT010727.1:CDS
GTAGTTTGCTAGACGCCGTC+CGG 0.538457 2:-77093168 None:intergenic
CAGGATTTAGACCTGCAGAA+AGG 0.540009 2:+77093135 MsaT010727.1:CDS
AACTTCCATTGGCAACTCCA+TGG 0.540581 2:-77093403 None:intergenic
GCAATGAAGTTGTATTCAAA+TGG 0.550757 2:-77093209 None:intergenic
AGTCGATGTGGATGGTAAGT+GGG 0.556259 2:+77093730 MsaT010727.1:CDS
CGGTGCGCTATAACTTCCAT+TGG 0.556645 2:-77093414 None:intergenic
TAATGTTGATTCAGGAGGAA+AGG 0.556753 2:+77092614 MsaT010727.1:CDS
AACACTAAAATCTTGCAGAA+AGG 0.557457 2:-77091866 None:intergenic
TTGTGCACATTGATTTAGCA+TGG 0.558170 2:-77092706 None:intergenic
ATGCCACAGTTTCATCAGGA+AGG 0.560068 2:-77093319 None:intergenic
CCAGCAGTTGCCACATCAAG+AGG 0.562934 2:-77092882 None:intergenic
GGTGCTAACCATTGTCATAA+CGG 0.564395 2:+77092354 MsaT010727.1:CDS
TGAGACCTACAAGCAGTGGC+AGG 0.569446 2:+77093610 MsaT010727.1:CDS
TCCAAGTTCCGTTATGACAA+TGG 0.569856 2:-77092362 None:intergenic
AACTGGATCAGCTTCTGTCA+AGG 0.571830 2:-77092316 None:intergenic
CATAAATGTTATAGCTTGTG+AGG 0.573605 2:+77093565 MsaT010727.1:CDS
CAGAGATTGATGCTTGAGAG+AGG 0.576892 2:+77093524 MsaT010727.1:CDS
AGGTCAGGAATAAGAGAGCT+GGG 0.580907 2:+77093630 MsaT010727.1:CDS
ATACATAAGTGACATCCTCA+TGG 0.582153 2:+77092014 MsaT010727.1:CDS
TGTAATGAACAAGAGGGAAG+AGG 0.582616 2:+77092480 MsaT010727.1:CDS
TATCAAACAACGATGAGAAG+TGG 0.582630 2:-77093469 None:intergenic
CACTCTCTGCAAAGGAGACG+CGG 0.582762 2:-77092937 None:intergenic
TGACATTCAGCCAGTGATGG+TGG 0.583132 2:+77092392 MsaT010727.1:CDS
GAATTTGCTATGGTTTCCAG+TGG 0.587108 2:+77093033 MsaT010727.1:CDS
CAAAGTAATGAGCTAAACGC+TGG 0.593615 2:-77092812 None:intergenic
AAACATTCCTGCAACATACA+GGG 0.594260 2:-77092063 None:intergenic
TGATACTTGTAATGAACAAG+AGG 0.595387 2:+77092473 MsaT010727.1:CDS
GTGCCAGAAGAAGGTGAAGA+TGG 0.595795 2:-77091937 None:intergenic
GAGGTACTGCTTTATCAGAA+AGG 0.596446 2:+77092558 MsaT010727.1:CDS
ATCATATCAGCCTCTTGATG+TGG 0.597759 2:+77092872 MsaT010727.1:CDS
GTATTTGTCACTCTCTGCAA+AGG 0.601498 2:-77092945 None:intergenic
ATTTAGACCTGCAGAAAGGG+TGG 0.601556 2:+77093139 MsaT010727.1:CDS
AGGGTGCAGAAAGGGTTGAA+AGG 0.602354 2:+77093585 MsaT010727.1:CDS
GATAGTCTTCTGACGAGGCA+CGG 0.602753 2:-77093056 None:intergenic
ACCTCATCTAAAATCTCTGG+TGG 0.604084 2:-77092540 None:intergenic
GCAGGTCTAAATCCTGGCCG+AGG 0.605176 2:-77093128 None:intergenic
TAGCTTGTGAGGGTGCAGAA+AGG 0.607236 2:+77093576 MsaT010727.1:CDS
AAGATTCGAATTCGACGACA+TGG 0.612871 2:-77092251 None:intergenic
CCAGTTGTCATCGAGACACA+AGG 0.613282 2:+77092333 MsaT010727.1:CDS
TGGGTTCAAGCAAATCAGGT+TGG 0.613823 2:+77093649 MsaT010727.1:CDS
ACAAGAGGGAAGAGGTAGCA+AGG 0.614187 2:+77092488 MsaT010727.1:CDS
TAGACCTGCAGAAAGGGTGG+AGG 0.621359 2:+77093142 MsaT010727.1:CDS
GGCCTGAGACCTACAAGCAG+TGG 0.622095 2:+77093606 MsaT010727.1:CDS
GATACTTGTAATGAACAAGA+GGG 0.631778 2:+77092474 MsaT010727.1:CDS
TCAGTGCTATCATTTGGTGA+AGG 0.638613 2:-77092138 None:intergenic
TGAAACGAAAACAATCGTGA+AGG 0.640625 2:+77093688 MsaT010727.1:CDS
GAATGACATTCAGCCAGTGA+TGG 0.641311 2:+77092389 MsaT010727.1:CDS
TCACATAATGTTGATTCAGG+AGG 0.642164 2:+77092609 MsaT010727.1:CDS
ATCTACTACAAACAATGCCG+CGG 0.648638 2:+77092668 MsaT010727.1:CDS
GGTCCACAAATCCACAGCCG+CGG 0.657187 2:-77092685 None:intergenic
GAACCTTCCTGATGAAACTG+TGG 0.660816 2:+77093316 MsaT010727.1:CDS
AAATCAATGTGCACAAGCTG+TGG 0.661819 2:+77092713 MsaT010727.1:CDS
TGATTCAGGAGGAAAGGCAA+AGG 0.662454 2:+77092620 MsaT010727.1:CDS
AGAGATTGATGCTTGAGAGA+GGG 0.685595 2:+77093525 MsaT010727.1:CDS
AGACTATCATTAAGACCTGG+TGG 0.687440 2:+77093071 MsaT010727.1:CDS
AGTCATCTTCTTCATCCATG+AGG 0.688746 2:-77092029 None:intergenic
AAAGGGTGGAGGAAACCGGA+CGG 0.693036 2:+77093153 MsaT010727.1:CDS
AGAAGACTATCATTAAGACC+TGG 0.694335 2:+77093068 MsaT010727.1:CDS
ATAAATGTTATAGCTTGTGA+GGG 0.694950 2:+77093566 MsaT010727.1:CDS
ATGGGAAACCATCTGCCTGG+AGG 0.697664 2:+77093238 MsaT010727.1:CDS
AATCTCTGCATGTCTTCACG+AGG 0.708631 2:-77093509 None:intergenic
TTAATGATAGTCTTCTGACG+AGG 0.747045 2:-77093061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 77091850 77093811 77091850 ID=MsaG010727
Chr2 mRNA 77091850 77093811 77091850 ID=MsaT010727.1;Parent=MsaG010727
Chr2 exon 77091850 77093811 77091850 ID=MsaT010727.1.exon1;Parent=MsaT010727.1
Chr2 CDS 77091850 77093811 77091850 ID=cds.MsaT010727.1;Parent=MsaT010727.1
Gene Sequence

>MsaT010727.1

ATGCTTTCTCCAAACACCTTTCTGCAAGATTTTAGTGTTCCAAACTCAGTAAACCAAGTAACAAATGGGTTTGAATTAGAAGATTCACCATCTTCACCTTCTTCTGGCACAAGCTCAAATGGAGAATCCTTGGAACCGACAAGATATTCCAATAACATCCTAAGATACATAAGTGACATCCTCATGGATGAAGAAGATGACTTGGAGCAAAATCCCTGTATGTTGCAGGAATGTTTAAGACTTCAGGCTGCTGAAAAGTCCTTCTATGATGTTCTTGGCCATAACCAGCCTTCACCAAATGATAGCACTGACCCAAATGGAAATTTCGATCGCGCTGCTAGTTTTGAGAGAAATGGCAGCAGCTGCATCGCTGGTAACTCATATGAATCTAACTGTGTCAACCATGTCGTCGAATTCGAATCTTCTTCCATACAACTACAAAGTCCTTTAATAGAAAAAAACTATGCCTTGACAGAAGCTGATCCAGTTGTCATCGAGACACAAGGTGCTAACCATTGTCATAACGGAACTTGGAGTTGGAATGACATTCAGCCAGTGATGGTGGAAGAAGTTTTACCTTCAACAGTACCAGAAAAAAGAAGTCGTAAGATGGATAATGATAATGATACTTGTAATGAACAAGAGGGAAGAGGTAGCAAGGTTTCAGCTATTTTCTCTGATGAATTAGAGCCACCAGAGATTTTAGATGAGGTACTGCTTTATCAGAAAGGAAAGACTCTCACTCAACACCAACAAGCATCACATAATGTTGATTCAGGAGGAAAGGCAAAGGAAACACATTCGCGTTTGAAGAAAGCATCTACTACAAACAATGCCGCGGCTGTGGATTTGTGGACCATGCTAAATCAATGTGCACAAGCTGTGGCGAGCTACGATCAAAGGAATACAGATGAGCTACTCAAACAGATTAGGCAACACTCTTTGTCTTTTGGGGATGGACTCCAGCGTTTAGCTCATTACTTTGCTAATGGTCTTGAGATAAGATTGGCTGCTGAGACGCCATCATATCAGCCTCTTGATGTGGCAACTGCTGGAGATATGTTGAAAGCTTACAAACTATTTGTTACCGCGTCTCCTTTGCAGAGAGTGACAAATACTTTGCTAACCAAAACGATTTTCAAAATAGTGAACAACGAGAGCAGCGTTCATATTATTGATTTTGGAATTTGCTATGGTTTCCAGTGGCCGTGCCTCGTCAGAAGACTATCATTAAGACCTGGTGGTCCTCCAAAGCTTCGAATTACAGGAATTGAGCTTCCTCGGCCAGGATTTAGACCTGCAGAAAGGGTGGAGGAAACCGGACGGCGTCTAGCAAACTACTGCAAGAAGTTCAATGTACCATTTGAATACAACTTCATTGCACAGAAATGGGAAACCATCTGCCTGGAGGATCTAAAGATAGACAGGAATGAAATAACTCTTGTTAGCTGTTTGTACAGACTGAAGAACCTTCCTGATGAAACTGTGGCATTGAATTGTCCAAGAGAAGCTGTGTTGAAGCTGATCAGGATGATTAATCCAAAAGTATTCTTCCATGGAGTTGCCAATGGAAGTTATAGCGCACCGTTTTTCCTGACTCGGTTTAAGGAAGCACTCTACCACTTCTCATCGTTGTTTGATATGTTTGAGGCTAACGTGCCTCGTGAAGACATGCAGAGATTGATGCTTGAGAGAGGGTTGTTTGGAAGAGATGCCATAAATGTTATAGCTTGTGAGGGTGCAGAAAGGGTTGAAAGGCCTGAGACCTACAAGCAGTGGCAGGTCAGGAATAAGAGAGCTGGGTTCAAGCAAATCAGGTTGGATTCTGATTTAGTGAATGAAACGAAAACAATCGTGAAGGAATACCATAAAGATTTTGTAGTCGATGTGGATGGTAAGTGGGTTTTGCAGGGATGGAAGGGACGCATTTTGAATGCTTTATCAGCTTGGGTTCCTGCTTAA
Protein sequence

>MsaT010727.1

MLSPNTFLQDFSVPNSVNQVTNGFELEDSPSSPSSGTSSNGESLEPTRYSNNILRYISDILMDEEDDLEQNPCMLQECLRLQAAEKSFYDVLGHNQPSPNDSTDPNGNFDRAASFERNGSSCIAGNSYESNCVNHVVEFESSSIQLQSPLIEKNYALTEADPVVIETQGANHCHNGTWSWNDIQPVMVEEVLPSTVPEKRSRKMDNDNDTCNEQEGRGSKVSAIFSDELEPPEILDEVLLYQKGKTLTQHQQASHNVDSGGKAKETHSRLKKASTTNNAAAVDLWTMLNQCAQAVASYDQRNTDELLKQIRQHSLSFGDGLQRLAHYFANGLEIRLAAETPSYQPLDVATAGDMLKAYKLFVTASPLQRVTNTLLTKTIFKIVNNESSVHIIDFGICYGFQWPCLVRRLSLRPGGPPKLRITGIELPRPGFRPAERVEETGRRLANYCKKFNVPFEYNFIAQKWETICLEDLKIDRNEITLVSCLYRLKNLPDETVALNCPREAVLKLIRMINPKVFFHGVANGSYSAPFFLTRFKEALYHFSSLFDMFEANVPREDMQRLMLERGLFGRDAINVIACEGAERVERPETYKQWQVRNKRAGFKQIRLDSDLVNETKTIVKEYHKDFVVDVDGKWVLQGWKGRILNALSAWVPA*