AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010768


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010768 MtrunA17_Chr2g0328901 95.759 448 16 2 1 448 1 445 0.0 883
MsaG010768 MtrunA17_Chr1g0201951 47.500 360 162 8 85 419 92 449 1.99e-95 294
MsaG010768 MtrunA17_Chr6g0463911 46.450 338 154 4 103 414 105 441 5.12e-83 262
MsaG010768 MtrunA17_Chr7g0265561 46.748 246 106 7 4 243 3 229 8.48e-60 202
MsaG010768 MtrunA17_Chr1g0179561 60.248 161 58 3 84 243 94 249 9.53e-56 192
MsaG010768 MtrunA17_Chr1g0179561 41.441 111 61 1 310 416 371 481 2.55e-23 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010768 AT1G21200.1 39.787 470 205 12 1 419 1 443 4.89e-89 278
MsaG010768 AT1G21200.2 39.787 470 205 12 1 419 1 443 4.89e-89 278
MsaG010768 AT1G76870.2 42.651 347 167 10 84 419 60 385 1.43e-71 231
MsaG010768 AT1G76870.1 42.651 347 167 10 84 419 60 385 1.43e-71 231
MsaG010768 AT1G76870.3 42.651 347 167 10 84 419 60 385 1.43e-71 231
MsaG010768 AT3G10040.1 41.152 243 126 4 11 243 6 241 2.21e-43 158

Find 116 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGATAATAATAATCAAAT+TGG 0.250764 2:-77556338 MsaT010768.1:CDS
TCAATTATGAATGGTTCTTC+TGG 0.281471 2:-77556494 None:intergenic
GGTTCTTCTGGTTTGGGTGG+TGG 0.300179 2:-77556482 MsaT010768.1:CDS
TATGAATGGTTCTTCTGGTT+TGG 0.302937 2:-77556489 MsaT010768.1:CDS
CAAGTCAAGATCATGACTAT+TGG 0.311830 2:+77555746 None:intergenic
ATGAATGGTTCTTCTGGTTT+GGG 0.316755 2:-77556488 MsaT010768.1:CDS
TTCTCCCACGGAGACTTTGC+AGG 0.318216 2:+77555474 None:intergenic
GTTCTTCTGGTTTGGGTGGT+GGG 0.328898 2:-77556481 MsaT010768.1:CDS
ACCAATGGAGGTAGGGTCTA+AGG 0.337698 2:+77555215 None:intergenic
GTGAGGAGGATGGCCACTTT+TGG 0.369260 2:-77555554 MsaT010768.1:CDS
GGAGAAAAGAGAGTGGATTA+AGG 0.381772 2:-77555457 MsaT010768.1:CDS
TATGAATATGATAACTGGTT+TGG 0.383314 2:-77556366 MsaT010768.1:CDS
GGATAGGGAGCTGGAGAAGC+TGG 0.386271 2:-77555334 MsaT010768.1:CDS
CAACATATGAATATGATAAC+TGG 0.387205 2:-77556371 MsaT010768.1:CDS
TCCTGCATTGATGGATTCAA+TGG 0.411710 2:-77555871 MsaT010768.1:CDS
GAGGATGATGAGTTGGATAA+TGG 0.423042 2:-77555642 MsaT010768.1:CDS
AAGTCAAGATCATGACTATT+GGG 0.423237 2:+77555747 None:intergenic
GAAGTTCAAAAGCCCTGGCT+TGG 0.432989 2:+77555397 None:intergenic
GAGCATGGTTACGGAGAAGA+TGG 0.435266 2:-77556224 MsaT010768.1:CDS
GATGGTGTTAAGAAGAAATC+CGG 0.439201 2:-77556062 MsaT010768.1:CDS
ATCCGGGGTGTCACAGAAGA+AGG 0.448557 2:-77556045 MsaT010768.1:CDS
TGGTAGGGGAACTTGTTGTC+AGG 0.449451 2:-77555904 MsaT010768.1:CDS
GAAGACGATGAGCATGGTTA+CGG 0.454149 2:-77556233 MsaT010768.1:CDS
GTGAGTTGTGTTGGTGATGA+TGG 0.455421 2:-77556101 MsaT010768.1:CDS
GAAGAAACTTGAGAGTGAAA+TGG 0.458951 2:-77555514 MsaT010768.1:CDS
GGTTGAGAATCCTGCATTGA+TGG 0.463782 2:-77555880 MsaT010768.1:CDS
GATACCGCAGCAAGAAGGAT+AGG 0.473004 2:-77555350 MsaT010768.1:CDS
TGACTTGCATAGTTATTCGT+TGG 0.479428 2:-77555730 MsaT010768.1:CDS
TTCAGAAACAGCGGTTTAAG+TGG 0.489001 2:-77555377 MsaT010768.1:CDS
ACCATTGAATCCATCAATGC+AGG 0.492133 2:+77555870 None:intergenic
AATATTAATGCACGTGGTGA+TGG 0.497668 2:-77555615 MsaT010768.1:CDS
GGTGATGGAGGGAGAATGGA+AGG 0.501886 2:-77555600 MsaT010768.1:CDS
TCTAAGATAATGATAAGTAA+AGG 0.502250 2:-77556005 MsaT010768.1:CDS
AATTGTGTTTCAATTATGAA+TGG 0.507262 2:-77556503 None:intergenic
TTGTGGCCTCTTGATACCAA+TGG 0.514883 2:+77555200 None:intergenic
TCTCCCACGGAGACTTTGCA+GGG 0.516340 2:+77555475 None:intergenic
AATGGTTCTTCTGGTTTGGG+TGG 0.516998 2:-77556485 MsaT010768.1:CDS
TCCTTAGACCCTACCTCCAT+TGG 0.518261 2:-77555216 MsaT010768.1:CDS
GGTTGAATGAGATGCTTGGT+AGG 0.519981 2:-77555920 MsaT010768.1:CDS
CATAGTTATTCGTTGGAACA+CGG 0.524019 2:-77555723 MsaT010768.1:CDS
AAAATGTTGACTTCCAAGCC+AGG 0.526284 2:-77555410 MsaT010768.1:CDS
CACTAGACTGCGGCCAAAAG+TGG 0.527780 2:+77555541 None:intergenic
AATGGTTAACTTGTCTGCTA+AGG 0.528317 2:-77555853 MsaT010768.1:CDS
TTTCTGAAGTTCAAAAGCCC+TGG 0.531742 2:+77555392 None:intergenic
GGTGATGATGGAACAATTAG+TGG 0.532799 2:-77556089 MsaT010768.1:CDS
TGCAATTTGAAAATGAGCAC+AGG 0.532921 2:-77555293 MsaT010768.1:CDS
GCCTATGCAATGGAGATTCA+AGG 0.537095 2:+77556426 None:intergenic
CAGCAAGAAGGATAGGGAGC+TGG 0.539921 2:-77555343 MsaT010768.1:CDS
TGGGTATCCTTTGACCATTG+TGG 0.541818 2:+77555766 None:intergenic
ATTAATGCACGTGGTGATGG+AGG 0.542789 2:-77555612 MsaT010768.1:CDS
TGAAGATAACAATGAAAGTG+AGG 0.549731 2:-77555661 MsaT010768.1:CDS
ATAGCAGTGGTGAGTTGTGT+TGG 0.551265 2:-77556110 MsaT010768.1:CDS
TAGGGGAACTTGTTGTCAGG+TGG 0.556404 2:-77555901 MsaT010768.1:CDS
TGAAAGTGAGGATGATGAGT+TGG 0.557152 2:-77555649 MsaT010768.1:CDS
AACTTGAGAGTGAAATGGCA+AGG 0.557312 2:-77555509 MsaT010768.1:CDS
GTTGAATGAGATGCTTGGTA+GGG 0.559093 2:-77555919 MsaT010768.1:CDS
TATCAAGAGGCCACAAGGGA+GGG 0.560647 2:-77555193 MsaT010768.1:CDS
ATACCGCAGCAAGAAGGATA+GGG 0.564239 2:-77555349 MsaT010768.1:CDS
AAAGCTGAAGCAGAGAGAGC+AGG 0.565355 2:-77555265 MsaT010768.1:CDS
TTGATATGTTCCCTCCCTTG+TGG 0.565958 2:+77555183 None:intergenic
GTCCACTTCATTCTCTGCCA+AGG 0.566514 2:+77556155 None:intergenic
ACTTGAATAAGAGATATAAG+AGG 0.568508 2:-77555941 MsaT010768.1:CDS
TGTCACAGAAGAAGGGTAAG+TGG 0.569426 2:-77556037 MsaT010768.1:CDS
TCTTGATACCAATGGAGGTA+GGG 0.573392 2:+77555208 None:intergenic
GGAGCTGGAGAAGCTGGCGA+TGG 0.574129 2:-77555328 MsaT010768.1:CDS
AAGAGGTTGAATGAGATGCT+TGG 0.576189 2:-77555924 MsaT010768.1:CDS
AGCAGGAGGCAGAGTTCAGT+AGG 0.578594 2:-77555248 MsaT010768.1:CDS
CAACAGACTCAGTTAGCTCA+TGG 0.578956 2:-77556398 MsaT010768.1:CDS
ATGGTGTTAAGAAGAAATCC+GGG 0.579854 2:-77556061 MsaT010768.1:CDS
CTCTTGATACCAATGGAGGT+AGG 0.581041 2:+77555207 None:intergenic
TTGAATGAGATGCTTGGTAG+GGG 0.587060 2:-77555918 MsaT010768.1:CDS
TAGAAATAAATTAAGTGAGG+AGG 0.587853 2:-77555568 MsaT010768.1:CDS
ACGTGGTGATGGAGGGAGAA+TGG 0.588300 2:-77555604 MsaT010768.1:CDS
ACCCTTCTTCTGTGACACCC+CGG 0.588573 2:+77556043 None:intergenic
TGATAGAAATAAATTAAGTG+AGG 0.588915 2:-77555571 MsaT010768.1:CDS
GTTAAGATACCGCAGCAAGA+AGG 0.589509 2:-77555355 MsaT010768.1:CDS
TTAATGCACGTGGTGATGGA+GGG 0.592885 2:-77555611 MsaT010768.1:CDS
AAATGTTGACTTCCAAGCCA+GGG 0.593930 2:-77555409 MsaT010768.1:CDS
TAACTTGTCTGCTAAGGCGA+AGG 0.599282 2:-77555847 MsaT010768.1:CDS
ATAGTTATTCGTTGGAACAC+GGG 0.600371 2:-77555722 MsaT010768.1:CDS
TTATTCGTTGGAACACGGGA+AGG 0.601197 2:-77555718 MsaT010768.1:CDS
GTGCATACCACAATGGTCAA+AGG 0.603169 2:-77555773 MsaT010768.1:CDS
TGATGGAACAATTAGTGGTG+TGG 0.604354 2:-77556084 MsaT010768.1:CDS
ACTTGAGAGTGAAATGGCAA+GGG 0.606060 2:-77555508 MsaT010768.1:CDS
AGTGGACGGATAATGTGGTT+GGG 0.610749 2:-77556139 MsaT010768.1:CDS
AAGTGGACGGATAATGTGGT+TGG 0.613391 2:-77556140 MsaT010768.1:CDS
GTATCAAGAGGCCACAAGGG+AGG 0.615865 2:-77555194 MsaT010768.1:CDS
AACTTATCCTCACACTGCTG+CGG 0.618536 2:+77555969 None:intergenic
CTACCTCCATTGGTATCAAG+AGG 0.620308 2:-77555206 MsaT010768.1:CDS
ACCTTGAATCTCCATTGCAT+AGG 0.622935 2:-77556427 MsaT010768.1:CDS
AGCATGGTTACGGAGAAGAT+GGG 0.623013 2:-77556223 MsaT010768.1:CDS
AGACCCTGCAAAGTCTCCGT+GGG 0.624160 2:-77555478 MsaT010768.1:CDS
GGAACAATTAGTGGTGTGGA+TGG 0.625621 2:-77556080 MsaT010768.1:CDS
AATAAATTAAGTGAGGAGGA+TGG 0.626359 2:-77555564 MsaT010768.1:CDS
AATGAAGTGGACGGATAATG+TGG 0.631463 2:-77556144 MsaT010768.1:CDS
AGGGAGGGAACATATCAACT+TGG 0.631664 2:-77555178 MsaT010768.1:CDS
CTCCTTGGCAGAGAATGAAG+TGG 0.631774 2:-77556157 MsaT010768.1:CDS
TGTGTCGCCGCAGCAGTGTG+AGG 0.632288 2:-77555976 MsaT010768.1:CDS
GCTCCCTATCCTTCTTGCTG+CGG 0.632768 2:+77555346 None:intergenic
GGTTGGGCTTCTTATAGCAG+TGG 0.632989 2:-77556123 MsaT010768.1:CDS
ATTGGTATCAAGAGGCCACA+AGG 0.633070 2:-77555198 MsaT010768.1:CDS
TTCTTCATCCCACTAGACTG+CGG 0.642211 2:+77555531 None:intergenic
GCTGAAGCAGAGAGAGCAGG+AGG 0.642379 2:-77555262 MsaT010768.1:CDS
ATGAGCGAAGACGATGAGCA+TGG 0.644222 2:-77556239 MsaT010768.1:CDS
TTGGCAGAGAATGAAGTGGA+CGG 0.645694 2:-77556153 MsaT010768.1:CDS
CTCCGTGGGAGAAAAGAGAG+TGG 0.647688 2:-77555464 MsaT010768.1:CDS
AAGACCCTGCAAAGTCTCCG+TGG 0.649124 2:-77555479 MsaT010768.1:CDS
TGGCCTCTTGATACCAATGG+AGG 0.649145 2:+77555203 None:intergenic
GTGATGGAGGGAGAATGGAA+GGG 0.653559 2:-77555599 MsaT010768.1:CDS
TCCGGGGTGTCACAGAAGAA+GGG 0.653871 2:-77556044 MsaT010768.1:CDS
TTGGTATCAAGAGGCCACAA+GGG 0.674612 2:-77555197 MsaT010768.1:CDS
GAAATGTGTGCATACCACAA+TGG 0.676978 2:-77555780 MsaT010768.1:CDS
TGTTGTTGATGCCTATGCAA+TGG 0.677807 2:+77556416 None:intergenic
GGAATTAATATTAATGCACG+TGG 0.680730 2:-77555621 MsaT010768.1:CDS
TGGTGTTAAGAAGAAATCCG+GGG 0.737936 2:-77556060 MsaT010768.1:CDS
AAGGACTCAAGAGATCACGA+TGG 0.786747 2:-77555699 MsaT010768.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 77555164 77556510 77555164 ID=MsaG010768
Chr2 mRNA 77555164 77556510 77555164 ID=MsaT010768.1;Parent=MsaG010768
Chr2 exon 77555164 77556510 77555164 ID=MsaT010768.1.exon1;Parent=MsaT010768.1
Chr2 CDS 77555164 77556510 77555164 ID=cds.MsaT010768.1;Parent=MsaT010768.1
Gene Sequence

>MsaT010768.1

ATGAATGGTTCTTCTGGTTTGGGTGGTGGGTTTTTGAGTGGTCCCGCTGGGGGTATTTTGGACCTTGAATCTCCATTGCATAGGCATCAACAACAGACTCAGTTAGCTCATGGTCAACAACATATGAATATGATAACTGGTTTGGAAAATGATGATAATAATAATCAAATTGGTTTAATTGAAGTGAAAAATTTGAATTTTGGTAAAGGAAAGGGAGTTGCTAGTTCTAATCATGATAATAGTAATGATATGAGCGAAGACGATGAGCATGGTTACGGAGAAGATGGGAATTGCGAGAATTTTTTCGATGGTGGGAAGGGGAAAAAAGGGTCTCCTTGGCAGAGAATGAAGTGGACGGATAATGTGGTTGGGCTTCTTATAGCAGTGGTGAGTTGTGTTGGTGATGATGGAACAATTAGTGGTGTGGATGGTGTTAAGAAGAAATCCGGGGTGTCACAGAAGAAGGGTAAGTGGAAAACTGTTTCTAAGATAATGATAAGTAAAGGTTGTCATGTGTCGCCGCAGCAGTGTGAGGATAAGTTCAATGACTTGAATAAGAGATATAAGAGGTTGAATGAGATGCTTGGTAGGGGAACTTGTTGTCAGGTGGTTGAGAATCCTGCATTGATGGATTCAATGGTTAACTTGTCTGCTAAGGCGAAGGATGATGTTAGAAAGATATTGAGCTCAAAACATTTGTTTTATAAAGAAATGTGTGCATACCACAATGGTCAAAGGATACCCAATAGTCATGATCTTGACTTGCATAGTTATTCGTTGGAACACGGGAAGGACTCAAGAGATCACGATGGCTCTGATGATGAAGATGAAGATAACAATGAAAGTGAGGATGATGAGTTGGATAATGGAATTAATATTAATGCACGTGGTGATGGAGGGAGAATGGAAGGGTTTTGTGATAGAAATAAATTAAGTGAGGAGGATGGCCACTTTTGGCCGCAGTCTAGTGGGATGAAGAAACTTGAGAGTGAAATGGCAAGGGTTTTTCAAGACCCTGCAAAGTCTCCGTGGGAGAAAAGAGAGTGGATTAAGGAACAGTTGTTGCAGCTCCAAGAGCAAAATGTTGACTTCCAAGCCAGGGCTTTTGAACTTCAGAAACAGCGGTTTAAGTGGTTAAGATACCGCAGCAAGAAGGATAGGGAGCTGGAGAAGCTGGCGATGGAGAACAAGAGAATGCAATTTGAAAATGAGCACAGGATCTTAAAGCTGAAGCAGAGAGAGCAGGAGGCAGAGTTCAGTAGGTCTGAGATGTCCTTAGACCCTACCTCCATTGGTATCAAGAGGCCACAAGGGAGGGAACATATCAACTTGGCTAGACAGCACTAG

Protein sequence

>MsaT010768.1

MNGSSGLGGGFLSGPAGGILDLESPLHRHQQQTQLAHGQQHMNMITGLENDDNNNQIGLIEVKNLNFGKGKGVASSNHDNSNDMSEDDEHGYGEDGNCENFFDGGKGKKGSPWQRMKWTDNVVGLLIAVVSCVGDDGTISGVDGVKKKSGVSQKKGKWKTVSKIMISKGCHVSPQQCEDKFNDLNKRYKRLNEMLGRGTCCQVVENPALMDSMVNLSAKAKDDVRKILSSKHLFYKEMCAYHNGQRIPNSHDLDLHSYSLEHGKDSRDHDGSDDEDEDNNESEDDELDNGININARGDGGRMEGFCDRNKLSEEDGHFWPQSSGMKKLESEMARVFQDPAKSPWEKREWIKEQLLQLQEQNVDFQARAFELQKQRFKWLRYRSKKDRELEKLAMENKRMQFENEHRILKLKQREQEAEFSRSEMSLDPTSIGIKRPQGREHINLARQH*