AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010770 MtrunA17_Chr2g0328921 99.054 317 1 1 1 317 1 315 0.0 655
MsaG010770 MtrunA17_Chr7g0258011 66.319 288 72 5 44 313 67 347 6.07e-133 381
MsaG010770 MtrunA17_Chr1g0173841 63.036 303 78 5 44 317 93 390 7.27e-133 383
MsaG010770 MtrunA17_Chr8g0352011 64.931 288 80 8 44 314 55 338 7.40e-127 365
MsaG010770 MtrunA17_Chr5g0422571 68.349 218 55 4 84 298 56 262 4.20e-108 316
MsaG010770 MtrunA17_Chr2g0321291 60.166 241 85 3 73 307 67 302 3.43e-105 310
MsaG010770 MtrunA17_Chr4g0026521 59.289 253 92 4 60 310 48 291 7.58e-103 303
MsaG010770 MtrunA17_Chr1g0198651 78.261 161 32 1 135 292 99 259 1.73e-95 284
MsaG010770 MtrunA17_Chr4g0053341 60.870 230 75 4 60 289 11 225 7.14e-94 278
MsaG010770 MtrunA17_Chr1g0200421 39.597 149 80 5 145 286 245 390 1.30e-25 106
MsaG010770 MtrunA17_Chr2g0320341 34.615 156 95 3 138 286 231 386 5.17e-22 95.5
MsaG010770 MtrunA17_Chr4g0029521 35.971 139 80 4 145 282 61 191 1.51e-19 89.0
MsaG010770 MtrunA17_Chr7g0268961 34.868 152 81 7 139 282 33 174 1.65e-19 89.0
MsaG010770 MtrunA17_Chr8g0342421 30.151 199 116 8 97 282 26 214 2.42e-19 88.6
MsaG010770 MtrunA17_Chr1g0199101 33.939 165 89 8 128 282 45 199 2.94e-19 88.2
MsaG010770 MtrunA17_Chr8g0382121 38.849 139 78 6 153 286 127 263 3.10e-19 86.7
MsaG010770 MtrunA17_Chr1g0151351 35.211 142 81 5 143 282 62 194 3.38e-19 87.8
MsaG010770 MtrunA17_Chr1g0181381 34.247 146 87 4 138 282 54 191 4.74e-19 87.4
MsaG010770 MtrunA17_Chr1g0185121 35.211 142 81 5 143 282 61 193 8.18e-19 86.7
MsaG010770 MtrunA17_Chr1g0163001 32.927 164 92 6 128 282 41 195 8.53e-19 86.7
MsaG010770 MtrunA17_Chr1g0210821 33.562 146 87 5 138 282 60 196 1.13e-18 86.7
MsaG010770 MtrunA17_Chr2g0326381 34.247 146 87 4 138 282 48 185 1.19e-18 85.9
MsaG010770 MtrunA17_Chr1g0191991 34.337 166 89 7 127 282 60 215 1.45e-18 86.3
MsaG010770 MtrunA17_Chr1g0162971 35.252 139 80 5 145 282 64 193 1.46e-18 85.9
MsaG010770 MtrunA17_Chr7g0263941 34.694 147 84 6 138 282 50 186 3.89e-18 84.3
MsaG010770 MtrunA17_Chr2g0330171 32.877 146 88 4 138 282 62 198 5.35e-18 84.3
MsaG010770 MtrunA17_Chr8g0373541 33.813 139 82 4 145 282 56 185 7.48e-18 84.0
MsaG010770 MtrunA17_Chr3g0123941 35.152 165 93 7 153 313 139 293 8.82e-18 82.8
MsaG010770 MtrunA17_Chr2g0324991 33.803 142 84 5 138 277 8 141 8.93e-17 76.3
MsaG010770 MtrunA17_Chr4g0040571 31.507 146 90 4 138 282 55 191 1.28e-16 80.1
MsaG010770 MtrunA17_Chr3g0141431 30.723 166 97 5 123 278 15 172 1.98e-16 79.3
MsaG010770 MtrunA17_Chr8g0375521 30.986 142 87 5 143 281 48 181 7.38e-13 68.9
MsaG010770 MtrunA17_Chr6g0479921 28.966 145 93 4 143 285 50 186 3.32e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010770 AT1G51220.1 61.801 322 95 8 6 310 27 337 3.07e-131 377
MsaG010770 AT3G57670.1 61.059 321 73 5 44 317 67 382 2.69e-127 369
MsaG010770 AT3G20880.1 61.462 301 82 7 36 310 118 410 9.49e-123 358
MsaG010770 AT1G13290.1 68.269 208 50 4 88 293 52 245 5.10e-102 301
MsaG010770 AT1G34790.1 68.159 201 63 1 90 289 83 283 3.99e-100 297
MsaG010770 AT1G08290.1 72.571 175 41 2 113 286 148 316 1.65e-94 284
MsaG010770 AT1G34370.3 37.209 172 89 6 131 286 7 175 2.45e-26 105
MsaG010770 AT1G34370.2 39.735 151 79 5 145 286 238 385 5.12e-26 108
MsaG010770 AT1G34370.1 39.735 151 79 5 145 286 238 385 5.12e-26 108
MsaG010770 AT5G22890.1 38.994 159 83 7 142 290 209 363 8.24e-23 97.8
MsaG010770 AT2G02080.1 31.088 193 113 6 101 282 23 206 1.46e-20 92.4
MsaG010770 AT2G02080.4 31.088 193 113 6 101 282 23 206 1.46e-20 92.4
MsaG010770 AT2G02080.5 31.088 193 113 6 101 282 23 206 1.46e-20 92.4
MsaG010770 AT2G02080.3 31.088 193 113 6 101 282 23 206 1.46e-20 92.4
MsaG010770 AT1G14580.1 34.320 169 93 5 123 282 46 205 5.56e-20 90.5
MsaG010770 AT1G14580.2 34.320 169 93 5 123 282 46 205 5.56e-20 90.5
MsaG010770 AT1G14580.3 34.320 169 93 5 123 282 60 219 6.76e-20 90.5
MsaG010770 AT1G55110.3 30.455 220 123 8 73 282 17 216 1.68e-19 89.0
MsaG010770 AT1G55110.1 30.455 220 123 8 73 282 17 216 1.68e-19 89.0
MsaG010770 AT1G55110.2 30.455 220 123 8 73 282 17 216 1.68e-19 89.0
MsaG010770 AT5G03150.1 35.252 139 81 4 145 282 76 206 2.28e-19 89.0
MsaG010770 AT5G66730.1 29.843 191 111 7 105 282 4 184 4.62e-19 87.8
MsaG010770 AT2G02070.2 34.247 146 86 4 138 282 68 204 7.95e-19 87.4
MsaG010770 AT2G02070.1 34.247 146 86 4 138 282 68 204 7.95e-19 87.4
MsaG010770 AT2G02080.6 34.783 138 80 4 146 282 1 129 3.28e-18 85.1
MsaG010770 AT2G02080.2 34.783 138 80 4 146 282 1 129 3.28e-18 85.1
MsaG010770 AT1G03840.2 33.562 146 87 5 138 282 55 191 1.69e-17 83.2
MsaG010770 AT1G03840.1 33.562 146 87 5 138 282 57 193 1.78e-17 83.2
MsaG010770 AT5G44160.1 33.094 139 83 4 145 282 60 189 2.21e-17 82.8
MsaG010770 AT3G45260.2 35.252 139 81 4 145 282 62 192 2.65e-17 82.4
MsaG010770 AT3G45260.1 35.252 139 81 4 145 282 62 192 2.65e-17 82.4
MsaG010770 AT5G44160.2 33.094 139 83 4 145 282 21 150 3.09e-17 82.4
MsaG010770 AT5G60470.2 34.074 135 81 3 145 278 67 194 3.24e-17 82.4
MsaG010770 AT5G60470.3 34.074 135 81 3 145 278 63 190 3.32e-17 82.4
MsaG010770 AT5G60470.1 34.074 135 81 3 145 278 58 185 3.56e-17 82.0
MsaG010770 AT3G50700.1 32.192 146 89 4 138 282 50 186 1.48e-16 80.5
MsaG010770 AT4G02670.1 32.192 146 90 3 138 282 69 206 1.94e-16 79.7
MsaG010770 AT4G02670.2 32.192 146 90 3 138 282 71 208 2.05e-16 79.7
MsaG010770 AT3G13810.5 32.192 146 90 4 138 282 81 218 3.89e-16 79.3
MsaG010770 AT3G13810.4 32.192 146 90 4 138 282 86 223 4.07e-16 79.3
MsaG010770 AT3G13810.1 32.192 146 90 4 138 282 86 223 4.07e-16 79.3
MsaG010770 AT3G13810.2 32.192 146 90 4 138 282 87 224 4.12e-16 79.3
MsaG010770 AT3G13810.3 32.192 146 90 4 138 282 73 210 4.17e-16 79.3
MsaG010770 AT3G13810.6 32.192 146 90 4 138 282 67 204 4.65e-16 79.0

Find 64 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGTTCCTCTTAGAGATTC+TGG 0.183675 2:-77577583 None:intergenic
CCAATGACTTCAATGGTTAT+TGG 0.191789 2:+77575606 MsaT010770.1:CDS
CAACACTTTCTACACATAAA+TGG 0.204972 2:-77577742 None:intergenic
AAAGATCATATCAAGGCTTT+TGG 0.250816 2:+77577868 MsaT010770.1:CDS
GGAAATGGACATAAAGCTTA+TGG 0.262958 2:+77577889 MsaT010770.1:CDS
TATAGAATGGAGGGTTATTT+AGG 0.265203 2:-77575634 None:intergenic
GTTACTGTGGCTTTGCATCT+AGG 0.282488 2:+77575851 MsaT010770.1:CDS
AAAGATATGTTGTTACTATA+AGG 0.326372 2:-77575662 None:intergenic
CATGTTGTTGTATCTATTGA+AGG 0.358064 2:-77576059 None:intergenic
CATCATTTCCATGGAACTAA+AGG 0.398872 2:-77575774 None:intergenic
AACTTTGGTATTGTTGTTGT+GGG 0.402964 2:+77577818 MsaT010770.1:CDS
CCAATAACCATTGAAGTCAT+TGG 0.409520 2:-77575606 None:intergenic
TGCAAAGCATTTGCAGTAAG+AGG 0.436467 2:+77577763 MsaT010770.1:CDS
AGAATCTTCCTTTAGTTCCA+TGG 0.448392 2:+77575766 MsaT010770.1:CDS
CTTCATATTCATCATTTCCA+TGG 0.449667 2:-77575783 None:intergenic
TTACTGTGGCTTTGCATCTA+GGG 0.458041 2:+77575852 MsaT010770.1:CDS
AAACTTTGGTATTGTTGTTG+TGG 0.458245 2:+77577817 MsaT010770.1:CDS
GTCCACCAAATAAAATTAGC+AGG 0.460803 2:+77575960 MsaT010770.1:CDS
CCATGTTATTGTTGTGCACC+TGG 0.466023 2:+77577628 MsaT010770.1:CDS
ATTGAAGTCATTGGAGAAGT+AGG 0.466777 2:-77575597 None:intergenic
ACCCCTGCTCAGATTCTAAT+CGG 0.468227 2:+77576001 MsaT010770.1:CDS
TCTCTTGGAGGTGGAGATGA+AGG 0.479256 2:-77575695 None:intergenic
AAAGGAAGAGCTTCTCTTGG+AGG 0.486389 2:-77575707 None:intergenic
AAATCAGATGAACTTATACT+TGG 0.487140 2:-77575878 None:intergenic
AAAATATATAGATGCATATG+TGG 0.490811 2:+77577533 MsaT010770.1:intron
AGCAAAGGAAGAGCTTCTCT+TGG 0.490887 2:-77575710 None:intergenic
TACTTCTCCAATGACTTCAA+TGG 0.492298 2:+77575599 MsaT010770.1:CDS
TTGAAGGTCTTGAAACAAAG+AGG 0.499110 2:-77576043 None:intergenic
AAATTAGCAGGGGTCAGTAT+TGG 0.506191 2:+77575972 MsaT010770.1:CDS
TAAGGAAAATTATAGAATGG+AGG 0.508828 2:-77575644 None:intergenic
GGACCGATTAGAATCTGAGC+AGG 0.523985 2:-77576004 None:intergenic
CATGGAAATGATGAATATGA+AGG 0.524476 2:+77575784 MsaT010770.1:CDS
GGAAGAGCTTCTCTTGGAGG+TGG 0.534319 2:-77575704 None:intergenic
ATCACTTAAAGATCATATCA+AGG 0.535427 2:+77577861 MsaT010770.1:CDS
ACATGGATCACAATATAGAA+AGG 0.537364 2:+77577558 MsaT010770.1:CDS
CTATAAGGAAAATTATAGAA+TGG 0.547263 2:-77575647 None:intergenic
CCAGGTGCACAACAATAACA+TGG 0.547993 2:-77577628 None:intergenic
AAATATATAGATGCATATGT+GGG 0.547995 2:+77577534 MsaT010770.1:intron
ATTAGAATCTGAGCAGGGGT+AGG 0.550899 2:-77575998 None:intergenic
AAGGAAAATTATAGAATGGA+GGG 0.551334 2:-77575643 None:intergenic
TTGAAGTCATTGGAGAAGTA+GGG 0.551474 2:-77575596 None:intergenic
TCAATGTTGTTCTTGCATCC+AGG 0.558572 2:-77577646 None:intergenic
AGAGGGCATGAAAACTGTGT+TGG 0.560971 2:-77576025 None:intergenic
CATGGATCACAATATAGAAA+GGG 0.565142 2:+77577559 MsaT010770.1:CDS
ACACATTACAAGAGAAAGCA+TGG 0.565234 2:+77577712 MsaT010770.1:CDS
GGGAGTCTTAGCATTGCTGT+TGG 0.566939 2:-77577607 None:intergenic
TTAGAATCTGAGCAGGGGTA+GGG 0.569587 2:-77575997 None:intergenic
TAGATGCATATGTGGGGACA+TGG 0.582440 2:+77577541 MsaT010770.1:intron
AAGGGACCAGAATCTCTAAG+AGG 0.583893 2:+77577577 MsaT010770.1:CDS
TCCACCAAATAAAATTAGCA+GGG 0.584942 2:+77575961 MsaT010770.1:CDS
AGTGTTCTAAAATCTTTGAG+TGG 0.612744 2:-77577685 None:intergenic
TGAAGGTCTTGAAACAAAGA+GGG 0.613383 2:-77576042 None:intergenic
GTAGGGCTTAGGTTAAGCAA+AGG 0.619991 2:-77575725 None:intergenic
GACCGATTAGAATCTGAGCA+GGG 0.623713 2:-77576003 None:intergenic
CATTTGCAGTAAGAGGTGAC+TGG 0.624837 2:+77577770 MsaT010770.1:CDS
AGATGATGATGCCGTTACTG+TGG 0.634808 2:+77575838 MsaT010770.1:CDS
CAATAGATACAACAACATGC+AGG 0.643534 2:+77576063 MsaT010770.1:CDS
TAGATGCAAAGCCACAGTAA+CGG 0.646015 2:-77575849 None:intergenic
CAAGATCAAGATGATGAACA+TGG 0.669051 2:+77577931 MsaT010770.1:CDS
AATATATAGATGCATATGTG+GGG 0.677061 2:+77577535 MsaT010770.1:intron
CCACCAAATAAAATTAGCAG+GGG 0.685279 2:+77575962 MsaT010770.1:CDS
ACCGATTAGAATCTGAGCAG+GGG 0.690964 2:-77576002 None:intergenic
CAGGTGCACAACAATAACAT+GGG 0.695885 2:-77577627 None:intergenic
GATCAAGATGATGAACATGG+TGG 0.762207 2:+77577934 MsaT010770.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 77575584 77577996 77575584 ID=MsaG010770
Chr2 mRNA 77575584 77577996 77575584 ID=MsaT010770.1;Parent=MsaG010770
Chr2 exon 77575584 77576084 77575584 ID=MsaT010770.1.exon1;Parent=MsaT010770.1
Chr2 CDS 77575584 77576084 77575584 ID=cds.MsaT010770.1;Parent=MsaT010770.1
Chr2 exon 77577544 77577996 77577544 ID=MsaT010770.1.exon2;Parent=MsaT010770.1
Chr2 CDS 77577544 77577996 77577544 ID=cds.MsaT010770.1;Parent=MsaT010770.1
Gene Sequence

>MsaT010770.1

ATGAGTACTCATCCCTACTTCTCCAATGACTTCAATGGTTATTGGTTTGACCTAAATAACCCTCCATTCTATAATTTTCCTTATAGTAACAACATATCTTTTGCATCTTCTCCTTCATCTCCACCTCCAAGAGAAGCTCTTCCTTTGCTTAACCTAAGCCCTACAAAACATGAAGATCTTCTAGAATCTTCCTTTAGTTCCATGGAAATGATGAATATGAAGGAGAAAGAACTAAGCATATCATCAAATTCTTTAGATGATGATGCCGTTACTGTGGCTTTGCATCTAGGGCTTCCAAGTATAAGTTCATCTGATTTAGCTTCTTCAAATATTTACAAAGATGATGAAAAAGTAGTGACTGTTGATTCTTCTGAGTGTCCACCAAATAAAATTAGCAGGGGTCAGTATTGGATCCCTACCCCTGCTCAGATTCTAATCGGTCCAACACAGTTTTCATGCCCTCTTTGTTTCAAGACCTTCAATAGATACAACAACATGCAGATGCATATGTGGGGACATGGATCACAATATAGAAAGGGACCAGAATCTCTAAGAGGAACACAACCAACAGCAATGCTAAGACTCCCATGTTATTGTTGTGCACCTGGATGCAAGAACAACATTGATCATCCAAGAGCAAAACCACTCAAAGATTTTAGAACACTTCAAACACATTACAAGAGAAAGCATGGAATAAAGCCATTTATGTGTAGAAAGTGTTGCAAAGCATTTGCAGTAAGAGGTGACTGGAGAACTCACGAGAAAAACTGTGGGAAACTTTGGTATTGTTGTTGTGGGTCCGATTTTAAGCACAAAAGATCACTTAAAGATCATATCAAGGCTTTTGGAAATGGACATAAAGCTTATGGAATTGACGAATGTCTTGATCAAGATCAAGATGATGAACATGGTGGCTCTGAAATTGAGCAAGACAATGAATCTTTTCAGTCATAG

Protein sequence

>MsaT010770.1

MSTHPYFSNDFNGYWFDLNNPPFYNFPYSNNISFASSPSSPPPREALPLLNLSPTKHEDLLESSFSSMEMMNMKEKELSISSNSLDDDAVTVALHLGLPSISSSDLASSNIYKDDEKVVTVDSSECPPNKISRGQYWIPTPAQILIGPTQFSCPLCFKTFNRYNNMQMHMWGHGSQYRKGPESLRGTQPTAMLRLPCYCCAPGCKNNIDHPRAKPLKDFRTLQTHYKRKHGIKPFMCRKCCKAFAVRGDWRTHEKNCGKLWYCCCGSDFKHKRSLKDHIKAFGNGHKAYGIDECLDQDQDDEHGGSEIEQDNESFQS*