AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010789

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010789 MtrunA17_Chr2g0329151 86.589 343 33 2 142 480 286 619 0.0 577
MsaG010789 MtrunA17_Chr2g0329151 97.931 145 3 0 1 145 1 145 9.36e-93 294
MsaG010789 MtrunA17_Chr2g0329141 65.359 153 44 3 332 480 35 182 3.23e-63 203
MsaG010789 MtrunA17_Chr2g0329141 85.294 34 5 0 44 77 1 34 5.96e-11 61.6
MsaG010789 MtrunA17_Chr5g0440591 65.926 135 43 2 24 158 35 166 1.77e-53 189
MsaG010789 MtrunA17_Chr3g0118361 68.333 120 35 2 39 158 53 169 1.15e-46 171
MsaG010789 MtrunA17_Chr3g0124291 58.407 113 38 2 35 145 29 134 1.19e-37 142
MsaG010789 MtrunA17_Chr8g0382561 58.333 120 42 3 32 149 16 129 4.63e-36 139
MsaG010789 MtrunA17_Chr6g0476291 67.213 61 19 1 94 154 1 60 4.36e-22 95.9
MsaG010789 MtrunA17_Chr6g0476301 65.574 61 20 1 94 154 1 60 1.04e-21 96.7
MsaG010789 MtrunA17_Chr6g0473191 67.797 59 18 1 94 152 1 58 3.34e-21 94.0
MsaG010789 MtrunA17_Chr7g0260281 55.556 63 28 0 83 145 1 63 6.21e-18 80.1
MsaG010789 MtrunA17_Chr3g0080401 45.000 100 44 4 94 193 1 89 8.44e-18 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010789 AT1G73730.1 73.793 145 38 0 1 145 1 145 8.75e-66 222
MsaG010789 AT1G73730.1 29.070 344 187 13 138 480 280 567 7.92e-11 64.7
MsaG010789 AT1G73730.2 78.906 128 27 0 18 145 6 133 1.68e-63 216
MsaG010789 AT1G73730.2 29.070 344 187 13 138 480 268 555 7.26e-11 64.7
MsaG010789 AT2G27050.1 63.433 134 48 1 25 158 36 168 1.47e-52 187
MsaG010789 AT3G20770.1 65.625 128 42 1 24 151 35 160 5.08e-51 183
MsaG010789 AT5G10120.1 54.310 116 51 1 29 144 14 127 4.73e-36 139
MsaG010789 AT5G65100.1 48.000 125 52 1 33 144 33 157 6.18e-32 129
MsaG010789 AT5G21120.3 53.968 126 57 1 31 155 41 166 1.58e-29 121
MsaG010789 AT5G21120.1 53.968 126 57 1 31 155 41 166 1.58e-29 121
MsaG010789 AT5G21120.2 53.968 126 57 1 31 155 48 173 1.81e-29 121

Find 130 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTCTCTGGTTGCCTAAT+TGG 0.150692 2:+77865402 None:intergenic
GGATCACCATTATTAGATTT+TGG 0.201596 2:-77865276 MsaT010789.1:CDS
ATGGGTGAACTCGTTGAAAT+TGG 0.238500 2:-77867945 MsaT010789.1:CDS
ATATTCGGCAGTTTACTTCT+AGG 0.240085 2:+77865849 None:intergenic
GGCCCCTCATGTCTTATTTC+CGG 0.240962 2:+77865741 None:intergenic
GTTGAAATTGGAGCTGATAT+TGG 0.286718 2:-77867933 MsaT010789.1:CDS
AGACAGGAGCAATCAGTTTA+TGG 0.290050 2:-77866021 MsaT010789.1:CDS
TCAGCTTCATGAGGCATAAC+AGG 0.297998 2:+77865516 None:intergenic
AGAGATTGAGGCAGAAGATT+TGG 0.298365 2:-77866939 MsaT010789.1:CDS
CCTGTTCATTCCCGTGAATC+TGG 0.310802 2:+77865793 None:intergenic
AACCCATGATATCATCAAAA+AGG 0.311917 2:+77865223 None:intergenic
CCAAACCTATCCAGCTCGTT+TGG 0.316285 2:+77865297 None:intergenic
TGTTGGTTCTGTTTCTTCTA+AGG 0.322080 2:-77866045 MsaT010789.1:CDS
TCGTCTCTCTCATTCATATT+CGG 0.345135 2:+77865834 None:intergenic
GAACGTGGTCGTTTCTTCCT+AGG 0.356496 2:+77865933 None:intergenic
TTTGATGATATCATGGGTTT+GGG 0.358361 2:-77865219 MsaT010789.1:CDS
TCTTAGCTCGATCGGAATCT+TGG 0.364311 2:+77865961 None:intergenic
TTGAAATTGGAGCTGATATT+GGG 0.378190 2:-77867932 MsaT010789.1:intron
AAAACTCCAAAATCTAATAA+TGG 0.379865 2:+77865270 None:intergenic
CCGGGTGCTGTTACATGAAC+TGG 0.385720 2:+77865639 None:intergenic
TTGATGTTGCCCATCCTGAC+TGG 0.393798 2:+77865458 None:intergenic
TTATCAGAGGAACCACTAAC+TGG 0.402423 2:+77866689 None:intergenic
AAGAAAAGGTGAAGTTTGAT+AGG 0.411593 2:-77866649 MsaT010789.1:CDS
GAGGGGCCAAGTCATCTATC+TGG 0.415007 2:-77865726 MsaT010789.1:CDS
ATAGTTATTGCAGCATTCTC+TGG 0.415425 2:+77865390 None:intergenic
TCACTGTTCGCGGCGGGACC+AGG 0.419875 2:+77865426 None:intergenic
CCAAACGAGCTGGATAGGTT+TGG 0.422592 2:-77865297 MsaT010789.1:CDS
ATGAATGGTGATGAAATTAT+CGG 0.435300 2:-77865354 MsaT010789.1:CDS
CCGCGAACAGTGAACCAATT+AGG 0.435891 2:-77865416 MsaT010789.1:CDS
CCTAATTGGTTCACTGTTCG+CGG 0.440195 2:+77865416 None:intergenic
TCATACTTGGCTATGGCTGC+AGG 0.444642 2:+77866620 None:intergenic
CTGTTTCTTCTAAGGAAGAC+AGG 0.448155 2:-77866037 MsaT010789.1:CDS
TACAATCCAGTCACAAGATA+TGG 0.449117 2:-77865483 MsaT010789.1:CDS
TATTAGATATCACAATATTC+CGG 0.450502 2:-77866981 MsaT010789.1:CDS
GGGGTGCCAGTTCATTTATC+AGG 0.451131 2:-77865672 MsaT010789.1:CDS
CAAACCTATCCAGCTCGTTT+GGG 0.454172 2:+77865298 None:intergenic
GAGGTGCCAGTTCATTTATC+AGG 0.455057 2:-77865699 MsaT010789.1:CDS
AAGATTTGGAGAGGCGGATG+TGG 0.460361 2:-77866925 MsaT010789.1:CDS
ATGAGACAGAATCTGCTTTG+AGG 0.472502 2:-77865767 MsaT010789.1:CDS
TCGCGAGTTATCTGATGGTT+CGG 0.474239 2:+77865995 None:intergenic
AGTAGCGATAACGGTACGTC+TGG 0.478350 2:-77866164 MsaT010789.1:CDS
CCAGATTCACGGGAATGAAC+AGG 0.482214 2:-77865793 MsaT010789.1:CDS
TCACCATTCATATTTGCTGC+AGG 0.483300 2:+77865366 None:intergenic
CCGTGTCCTGATAAATGAAC+TGG 0.486748 2:+77865666 None:intergenic
CCGTGTCCTGATAAATGAAC+TGG 0.486748 2:+77865693 None:intergenic
CCAGTTCATGTAACAGCACC+CGG 0.490378 2:-77865639 MsaT010789.1:CDS
CACAAGATATGGTCCCAGTC+AGG 0.497696 2:-77865472 MsaT010789.1:CDS
ATTGGTTCACTGTTCGCGGC+GGG 0.497840 2:+77865420 None:intergenic
CATTCCCAAACGAGCTGGAT+AGG 0.500944 2:-77865302 MsaT010789.1:CDS
TAAGTACATGCTGAAGCTCA+TGG 0.501457 2:-77866765 MsaT010789.1:CDS
TCATCACTGACATCTTTCTC+CGG 0.504020 2:+77866962 None:intergenic
AAGAACACCAGTTGGCATAT+CGG 0.504528 2:+77866136 None:intergenic
ATTTCATTGTCAGATTGTGC+TGG 0.504859 2:+77865885 None:intergenic
GGAATTACCGATATGCCAAC+TGG 0.505544 2:-77866143 MsaT010789.1:CDS
GATGAAATGATGCAGTACTT+TGG 0.511735 2:-77865192 MsaT010789.1:CDS
CCAGTTCATTTATCAGGACA+CGG 0.512600 2:-77865666 MsaT010789.1:CDS
CCAGTTCATTTATCAGGACA+CGG 0.512600 2:-77865693 MsaT010789.1:CDS
TAGAGCTTGGTGGAAAGAAA+AGG 0.512695 2:-77866663 MsaT010789.1:CDS
CTGATAACATTAGAGCTTGG+TGG 0.517273 2:-77866673 MsaT010789.1:CDS
TGTCAGTGATGAAGAGATTG+AGG 0.518821 2:-77866951 MsaT010789.1:CDS
GGAAGAAACGACCACGTTCG+AGG 0.522146 2:-77865929 MsaT010789.1:CDS
CTGGGACCATATCTTGTGAC+TGG 0.525198 2:+77865477 None:intergenic
GCTAGCAACTATGATGTTGA+TGG 0.525679 2:-77866080 MsaT010789.1:CDS
CTCTGCTTCATACTTGGCTA+TGG 0.528334 2:+77866613 None:intergenic
AAATGTCGAGGGCACAAGAT+GGG 0.530650 2:-77866793 MsaT010789.1:CDS
GAGAAGGGAAAACCAGTTAG+TGG 0.532079 2:-77866701 MsaT010789.1:CDS
GTAGCTGCACTCCAGATTCA+CGG 0.536625 2:-77865804 MsaT010789.1:CDS
AGAATCTGCTTTGAGGCCGC+CGG 0.545291 2:-77865760 MsaT010789.1:CDS
TTCCTTCGCGAGTTATCTGA+TGG 0.548644 2:+77865990 None:intergenic
AGGCAGCCTAGTAGCGATAA+CGG 0.550005 2:-77866173 MsaT010789.1:CDS
CAACATCAACATCTGCAGCC+TGG 0.553510 2:-77865444 MsaT010789.1:CDS
AGATATGGTCCCAGTCAGGA+TGG 0.555307 2:-77865468 MsaT010789.1:CDS
AAAAGGAATGATGGTGATGG+TGG 0.555478 2:+77865240 None:intergenic
TTTGGAGAGGCGGATGTGGA+AGG 0.556184 2:-77866921 MsaT010789.1:CDS
AGACACATTCCCAAACGAGC+TGG 0.557596 2:-77865307 MsaT010789.1:CDS
TTGCTAGCACTACTAGCAGC+AGG 0.559279 2:+77866095 None:intergenic
CCTCTGATAACATTAGAGCT+TGG 0.561811 2:-77866676 MsaT010789.1:CDS
CAGCTTCATGAGGCATAACA+GGG 0.562451 2:+77865517 None:intergenic
TGCAAACAGTATGATTTCCT+CGG 0.564591 2:-77865592 MsaT010789.1:CDS
TGATGTTGCCCATCCTGACT+GGG 0.565505 2:+77865459 None:intergenic
TAGCTGCACTCCAGATTCAC+GGG 0.566232 2:-77865803 MsaT010789.1:CDS
AGGATCGTATCAAACTCAAA+AGG 0.568104 2:-77866901 MsaT010789.1:CDS
GTATGGAATCATTCCTGAGA+AGG 0.572173 2:-77866717 MsaT010789.1:CDS
AAAGCTCGTGGATTTGTGTA+TGG 0.575890 2:-77866734 MsaT010789.1:CDS
CAGTTCATTTATCAGGACAC+GGG 0.576276 2:-77865665 MsaT010789.1:CDS
CAGTTCATTTATCAGGACAC+GGG 0.576276 2:-77865692 MsaT010789.1:CDS
AGGTTGCTTCTTAGCTCGAT+CGG 0.579443 2:+77865953 None:intergenic
TATGGAATCATTCCTGAGAA+GGG 0.580239 2:-77866716 MsaT010789.1:CDS
GGTGATGAAATTATCGGAGG+AGG 0.587365 2:-77865348 MsaT010789.1:CDS
TCGCTTCCAGATAGATGACT+TGG 0.588209 2:+77865720 None:intergenic
ATGGAAGTGTGCAAAGCTCG+TGG 0.588400 2:-77866746 MsaT010789.1:CDS
CCCTCATGTCTTATTTCCGG+CGG 0.590753 2:+77865744 None:intergenic
ATGCCTGCAGCAAATATGAA+TGG 0.596398 2:-77865369 MsaT010789.1:CDS
ATAAGAGTAATGATCAAACC+CGG 0.606820 2:+77865620 None:intergenic
AAGGTGAAGTTTGATAGGAA+TGG 0.608844 2:-77866644 MsaT010789.1:CDS
AAAATGTCGAGGGCACAAGA+TGG 0.611433 2:-77866794 MsaT010789.1:CDS
AACTGAGAATAGTGCAGCGG+TGG 0.612241 2:+77865546 None:intergenic
CCCTAGAGAAGCAAAAGCCG+AGG 0.615253 2:-77866844 MsaT010789.1:CDS
GATGGTACCGACGATGGTGT+TGG 0.615254 2:-77866062 MsaT010789.1:CDS
AAAGTGTGTATATGAGTAGA+CGG 0.616455 2:-77865569 MsaT010789.1:CDS
GATATGGTCCCAGTCAGGAT+GGG 0.617269 2:-77865467 MsaT010789.1:CDS
CCGCCGGAAATAAGACATGA+GGG 0.619238 2:-77865744 MsaT010789.1:CDS
AATTGGTTCACTGTTCGCGG+CGG 0.624847 2:+77865419 None:intergenic
TATCAAACTCAAAAGGCTCA+AGG 0.625808 2:-77866894 MsaT010789.1:CDS
CCAAGCTCTAATGTTATCAG+AGG 0.626452 2:+77866676 None:intergenic
TCAAGAAGAAGAACACCAGT+TGG 0.627033 2:+77866128 None:intergenic
AACAGAACCAACACCATCGT+CGG 0.629982 2:+77866055 None:intergenic
ACTCATATACACACTTTCCG+AGG 0.634311 2:+77865575 None:intergenic
AGGCAGAAGATTTGGAGAGG+CGG 0.634433 2:-77866931 MsaT010789.1:CDS
GACGTACCGTTATCGCTACT+AGG 0.635231 2:+77866167 None:intergenic
CTCGTAGGAAGAAAATGTCG+AGG 0.637174 2:-77866805 MsaT010789.1:CDS
GCCGCCGGAAATAAGACATG+AGG 0.637786 2:-77865745 MsaT010789.1:CDS
GCTGCACTTGACCTCGAACG+TGG 0.640254 2:+77865918 None:intergenic
TTGAGGCAGAAGATTTGGAG+AGG 0.640791 2:-77866934 MsaT010789.1:CDS
CAGTTCATGTAACAGCACCC+GGG 0.645071 2:-77865638 MsaT010789.1:CDS
AGATTGTGCTGGTAATGTGT+CGG 0.646269 2:+77865896 None:intergenic
AGTCATCTGATCAAGCTCGT+AGG 0.652332 2:-77866820 MsaT010789.1:CDS
AAGTCATCTATCTGGAAGCG+AGG 0.655312 2:-77865718 MsaT010789.1:CDS
ATCGAGCTAAGAAGCAACCT+AGG 0.657044 2:-77865950 MsaT010789.1:CDS
TGCAACTGAGAATAGTGCAG+CGG 0.657420 2:+77865543 None:intergenic
AACCATCAGATAACTCGCGA+AGG 0.657732 2:-77865992 MsaT010789.1:CDS
TCGTAGGAAGAAAATGTCGA+GGG 0.660921 2:-77866804 MsaT010789.1:CDS
GCTTGATCAGATGACTGCCT+CGG 0.661645 2:+77866827 None:intergenic
AGATTATAATCAGCTTCATG+AGG 0.669056 2:+77865507 None:intergenic
CGCCGGAAATAAGACATGAG+GGG 0.670859 2:-77865743 MsaT010789.1:CDS
TAAGAGTAATGATCAAACCC+GGG 0.678500 2:+77865621 None:intergenic
AATGGTGATGAAATTATCGG+AGG 0.683666 2:-77865351 MsaT010789.1:CDS
AGTTCATTTATCAGGACACG+GGG 0.705461 2:-77865664 MsaT010789.1:CDS
AGTTCATTTATCAGGACACG+GGG 0.705461 2:-77865691 MsaT010789.1:CDS
GATGTTGATGGTACCGACGA+TGG 0.714346 2:-77866068 MsaT010789.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 77865185 77867967 77865185 ID=MsaG010789
Chr2 mRNA 77865185 77867967 77865185 ID=MsaT010789.1;Parent=MsaG010789
Chr2 exon 77865185 77866193 77865185 ID=MsaT010789.1.exon3;Parent=MsaT010789.1
Chr2 CDS 77865185 77866193 77865185 ID=cds.MsaT010789.1;Parent=MsaT010789.1
Chr2 exon 77866626 77867024 77866626 ID=MsaT010789.1.exon2;Parent=MsaT010789.1
Chr2 CDS 77866626 77867024 77866626 ID=cds.MsaT010789.1;Parent=MsaT010789.1
Chr2 exon 77867933 77867967 77867933 ID=MsaT010789.1.exon1;Parent=MsaT010789.1
Chr2 CDS 77867933 77867967 77867933 ID=cds.MsaT010789.1;Parent=MsaT010789.1
Gene Sequence

>MsaT010789.1

ATGGGTGAACTCGTTGAAATTGGAGCTGATATTGGCTCAGATTTAGATGTCGACGATATTAGATATCACAATATTCCGGAGAAAGATGTCAGTGATGAAGAGATTGAGGCAGAAGATTTGGAGAGGCGGATGTGGAAGGATCGTATCAAACTCAAAAGGCTCAAGGAAAAACAAAAGCTTGCAGCACAGCAAGCCCTAGAGAAGCAAAAGCCGAGGCAGTCATCTGATCAAGCTCGTAGGAAGAAAATGTCGAGGGCACAAGATGGGATTCTTAAGTACATGCTGAAGCTCATGGAAGTGTGCAAAGCTCGTGGATTTGTGTATGGAATCATTCCTGAGAAGGGAAAACCAGTTAGTGGTTCCTCTGATAACATTAGAGCTTGGTGGAAAGAAAAGGTGAAGTTTGATAGGAATGGTCCTGCAGCCATAGCCAAGCAGCCTAGTAGCGATAACGGTACGTCTGGAATTACCGATATGCCAACTGGTGTTCTTCTTCTTGAGTATAAGCAACCTGCTGCTAGTAGTGCTAGCAACTATGATGTTGATGGTACCGACGATGGTGTTGGTTCTGTTTCTTCTAAGGAAGACAGGAGCAATCAGTTTATGGATGCCGAACCATCAGATAACTCGCGAAGGAATTCTGTCCAAGATTCCGATCGAGCTAAGAAGCAACCTAGGAAGAAACGACCACGTTCGAGGTCAAGTGCAGCCGACACATTACCAGCACAATCTGACAATGAAATTTTGCATGTTGAACCTAGAAGTAAACTGCCGAATATGAATGAGAGAGACGAAACACATGTAGCTGCACTCCAGATTCACGGGAATGAACAGGTCAATGAGACAGAATCTGCTTTGAGGCCGCCGGAAATAAGACATGAGGGGCCAAGTCATCTATCTGGAAGCGAGGTGCCAGTTCATTTATCAGGACACGGGGTGCCAGTTCATTTATCAGGACACGGGGTGCCAGTTCATGTAACAGCACCCGGGTTTGATCATTACTCTTATATGCATGCAAACAGTATGATTTCCTCGGAAAGTGTGTATATGAGTAGACGGCCACCGCTGCACTATTCTCAGTTGCAAAACCCTGTTATGCCTCATGAAGCTGATTATAATCTGTACAATCCAGTCACAAGATATGGTCCCAGTCAGGATGGGCAACATCAACATCTGCAGCCTGGTCCCGCCGCGAACAGTGAACCAATTAGGCAACCAGAGAATGCTGCAATAACTATGCCTGCAGCAAATATGAATGGTGATGAAATTATCGGAGGAGGCTTGAATTATCTTGCTAAAGACACATTCCCAAACGAGCTGGATAGGTTTGGATCACCATTATTAGATTTTGGAGTTTTTGAGAATCCACCATCACCATCATTCCTTTTTGATGATATCATGGGTTTGGGTGACGATGAAATGATGCAGTACTTTGGAGCATAG
Protein sequence

>MsaT010789.1

MGELVEIGADIGSDLDVDDIRYHNIPEKDVSDEEIEAEDLERRMWKDRIKLKRLKEKQKLAAQQALEKQKPRQSSDQARRKKMSRAQDGILKYMLKLMEVCKARGFVYGIIPEKGKPVSGSSDNIRAWWKEKVKFDRNGPAAIAKQPSSDNGTSGITDMPTGVLLLEYKQPAASSASNYDVDGTDDGVGSVSSKEDRSNQFMDAEPSDNSRRNSVQDSDRAKKQPRKKRPRSRSSAADTLPAQSDNEILHVEPRSKLPNMNERDETHVAALQIHGNEQVNETESALRPPEIRHEGPSHLSGSEVPVHLSGHGVPVHLSGHGVPVHVTAPGFDHYSYMHANSMISSESVYMSRRPPLHYSQLQNPVMPHEADYNLYNPVTRYGPSQDGQHQHLQPGPAANSEPIRQPENAAITMPAANMNGDEIIGGGLNYLAKDTFPNELDRFGSPLLDFGVFENPPSPSFLFDDIMGLGDDEMMQYFGA*