AlfalfaGEDB | MsaG010824

M. sativa ssp. caerulea / MsaG010824

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010824	XP_003597529.1	97.680	431	8	1	1	431	1	429	0.0	861

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010824	sp\|Q04088\|POF21_ARATH	66.027	365	93	10	1	361	1	338	3.37e-150	435

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010824	tr\|G7IHV8\|G7IHV8_MEDTR	97.680	431	8	1	1	431	1	429	0.0	861

TFs/TRs:

Gene ID	Type	Classification
MsaG010824	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000274	MsaG010824	0.805547	2.142649e-49	3.289811e-47
MsaG000584	MsaG010824	0.825936	6.348677e-54	1.638996e-51
MsaG000700	MsaG010824	0.813070	5.308894e-51	9.777351e-49
MsaG001132	MsaG010824	0.819348	2.128306e-52	4.601367e-50
MsaG003721	MsaG010824	0.801559	1.426013e-48	1.997125e-46
MsaG003870	MsaG010824	0.808103	6.212234e-50	1.013459e-47
MsaG004275	MsaG010824	0.803228	6.483036e-49	9.433565e-47
MsaG004361	MsaG010824	0.815033	1.967312e-51	3.806154e-49
MsaG004428	MsaG010824	0.800855	1.983620e-48	2.734197e-46
MsaG004431	MsaG010824	0.824194	1.630472e-53	4.012127e-51
MsaG004432	MsaG010824	0.801654	1.363229e-48	1.913380e-46
MsaG004447	MsaG010824	0.843220	2.986293e-58	1.291345e-55
MsaG005063	MsaG010824	0.823283	2.658535e-53	6.382591e-51
MsaG005132	MsaG010824	0.830529	5.021435e-55	1.475296e-52
MsaG005224	MsaG010824	0.822841	3.366982e-53	7.987081e-51
MsaG005544	MsaG010824	0.826998	3.555316e-54	9.452878e-52
MsaG005560	MsaG010824	0.823224	2.743463e-53	6.575683e-51
MsaG005564	MsaG010824	0.807232	9.493784e-50	1.516745e-47
MsaG005625	MsaG010824	0.848201	1.348503e-59	6.875130e-57
MsaG006256	MsaG010824	0.804440	3.640739e-49	5.448309e-47
MsaG006339	MsaG010824	0.837541	8.974199e-57	3.245414e-54
MsaG006387	MsaG010824	0.811139	1.392871e-50	2.445681e-48
MsaG006430	MsaG010824	0.815678	1.416113e-51	2.784902e-49
MsaG006725	MsaG010824	0.829949	6.946954e-55	2.007599e-52
MsaG006901	MsaG010824	0.810181	2.239655e-50	3.841501e-48
MsaG007196	MsaG010824	0.822058	5.108732e-53	1.186587e-50
MsaG007413	MsaG010824	0.811123	1.404410e-50	2.464945e-48
MsaG007553	MsaG010824	0.876966	1.912335e-68	3.002850e-65
MsaG007601	MsaG010824	0.812697	6.402294e-51	1.168164e-48
MsaG007602	MsaG010824	0.817260	6.290761e-52	1.288273e-49
MsaG007858	MsaG010824	0.804729	3.171367e-49	4.777785e-47
MsaG007988	MsaG010824	0.822770	3.497998e-53	8.282171e-51
MsaG008442	MsaG010824	0.834596	4.976600e-56	1.647012e-53
MsaG008405	MsaG010824	0.829245	1.028406e-54	2.912305e-52
MsaG010824	MsaG011506	0.800115	2.801941e-48	3.798163e-46
MsaG010824	MsaG011764	0.811516	1.154795e-50	2.046473e-48
MsaG010824	MsaG013310	0.835525	2.909936e-56	9.901878e-54
MsaG010824	MsaG013659	0.806252	1.525527e-49	2.381660e-47
MsaG010824	MsaG014042	0.828288	1.747879e-54	4.818512e-52
MsaG010824	MsaG014291	0.843116	3.183607e-58	1.372104e-55
MsaG010824	MsaG014314	0.833459	9.555916e-56	3.057619e-53
MsaG010824	MsaG015193	0.807482	8.405909e-50	1.351031e-47
MsaG010824	MsaG015782	0.804958	2.842060e-49	4.304584e-47
MsaG010824	MsaG017202	0.801134	1.740033e-48	2.413618e-46
MsaG010824	MsaG018045	0.802902	7.565773e-49	1.092614e-46
MsaG010824	MsaG018251	0.851021	2.222114e-60	1.247427e-57
MsaG010824	MsaG018479	0.858429	1.620181e-62	1.187220e-59
MsaG010824	MsaG019017	0.827066	3.425786e-54	9.125617e-52
MsaG010824	MsaG019089	0.818470	3.363780e-52	7.106599e-50
MsaG010824	MsaG019620	0.810632	1.791331e-50	3.106598e-48
MsaG010824	MsaG019783	0.812619	6.657376e-51	1.212328e-48
MsaG010824	MsaG022048	0.816320	1.020033e-51	2.038955e-49
MsaG010824	MsaG022056	0.807486	8.390261e-50	1.348630e-47
MsaG010824	MsaG022263	0.809840	2.649470e-50	4.507199e-48
MsaG010824	MsaG022437	0.808418	5.324938e-50	8.753144e-48
MsaG010824	MsaG022737	0.803217	6.518895e-49	9.483049e-47
MsaG010824	MsaG025845	0.834848	4.304522e-56	1.435343e-53
MsaG010824	MsaG025861	0.800212	2.677970e-48	3.638013e-46
MsaG010824	MsaG028336	0.866262	6.504899e-65	6.464194e-62
MsaG010824	MsaG028423	0.805065	2.699998e-49	4.099579e-47
MsaG010824	MsaG028719	0.800005	2.949070e-48	3.987899e-46
MsaG010824	MsaG029595	0.837969	6.976493e-57	2.556278e-54
MsaG010824	MsaG029710	0.807089	1.017587e-49	1.620276e-47
MsaG010824	MsaG030305	0.816575	8.947312e-52	1.800219e-49
MsaG010824	MsaG030510	0.800547	2.290712e-48	3.135667e-46
MsaG010824	MsaG031814	0.803065	7.006130e-49	1.015597e-46
MsaG010824	MsaG033712	0.801120	1.752051e-48	2.429482e-46
MsaG010824	MsaG036289	0.803686	5.217179e-49	7.672205e-47
MsaG010824	MsaG036568	0.853804	3.610530e-61	2.235140e-58
MsaG010824	MsaG038011	0.814212	2.984406e-51	5.655818e-49
MsaG010824	MsaG040343	0.820759	1.015709e-52	2.279123e-50
MsaG010824	MsaG041597	0.807959	6.665423e-50	1.083624e-47
MsaG010824	MsaG042178	0.802713	8.273854e-49	1.189692e-46
MsaG010824	MsaG044115	0.800722	2.110805e-48	2.900889e-46
MsaG010824	MsaG044522	0.805985	1.735202e-49	2.691757e-47
MsaG010824	MsaG044614	0.814453	2.640909e-51	5.035236e-49
MsaG010824	MsaG044668	0.811796	1.004507e-50	1.792424e-48
MsaG010824	MsaG045357	0.803120	6.826073e-49	9.907662e-47
MsaG010824	MsaG045945	0.806330	1.469195e-49	2.297927e-47
MsaG010824	MsaG046268	0.809308	3.443805e-50	5.783253e-48
MsaG010824	MsaG046411	0.821534	6.744137e-53	1.544737e-50
MsaG010824	MsaG046749	0.801216	1.674391e-48	2.326836e-46
MsaG010824	MsaG008180	0.817764	4.847834e-52	1.005716e-49
MsaG010824	MsaG011491	0.805750	1.943201e-49	2.997882e-47
MsaG010824	MsaG027945	0.828104	1.934789e-54	5.306468e-52
MsaG010824	MsaG033142	0.803896	4.719628e-49	6.974263e-47
MsaG010824	MsaG031626	0.836106	2.076283e-56	7.189410e-54
MsaG010824	MsaG032369	0.812118	8.553645e-51	1.538377e-48
MsaG010824	MsaG031977	0.823315	2.613473e-53	6.279650e-51
MsaG010824	MsaG032111	0.828378	1.663581e-54	4.597971e-52
MsaG010824	MsaG031289	0.801223	1.669595e-48	2.320469e-46
MsaG010824	MsaG038223	0.849122	7.512579e-60	3.951742e-57
MsaG010824	MsaG036813	0.832301	1.848548e-55	5.717461e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010824	MtrunA17_Chr2g0329551	97.680	431	8	1	1	431	1	429	0.0	861
MsaG010824	MtrunA17_Chr3g0107491	45.115	348	142	10	72	406	38	349	5.92e-66	215
MsaG010824	MtrunA17_Chr5g0432701	52.941	255	67	9	72	320	51	258	5.55e-65	212
MsaG010824	MtrunA17_Chr3g0128781	44.161	274	79	8	72	335	7	216	3.86e-51	174
MsaG010824	MtrunA17_Chr4g0036071	44.318	264	88	8	109	323	213	466	2.24e-49	175
MsaG010824	MtrunA17_Chr6g0463571	42.208	308	123	12	66	366	22	281	1.45e-47	165
MsaG010824	MtrunA17_Chr1g0156051	59.712	139	53	2	176	313	343	479	1.49e-32	129
MsaG010824	MtrunA17_Chr2g0316551	38.462	156	81	2	219	374	206	346	1.39e-20	92.4
MsaG010824	MtrunA17_Chr2g0325131	45.614	114	57	2	219	327	189	302	1.22e-19	89.7
MsaG010824	MtrunA17_Chr4g0040891	46.000	100	54	0	219	318	192	291	6.93e-19	87.4
MsaG010824	MtrunA17_Chr1g0202051	37.615	109	68	0	206	314	109	217	1.73e-13	70.5
MsaG010824	MtrunA17_Chr8g0380611	27.341	267	158	6	58	313	80	321	2.03e-12	68.2
MsaG010824	MtrunA17_Chr2g0306581	63.265	49	18	0	209	257	1	49	2.03e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG010824	AT1G06070.1	73.512	336	70	6	19	352	21	339	6.28e-168	478
MsaG010824	AT2G31370.5	66.027	365	93	10	1	361	1	338	1.18e-151	435
MsaG010824	AT2G31370.2	66.027	365	93	10	1	361	1	338	3.42e-151	435
MsaG010824	AT2G31370.4	66.027	365	93	10	1	361	1	338	3.42e-151	435
MsaG010824	AT2G31370.1	66.027	365	93	10	1	361	1	338	3.42e-151	435
MsaG010824	AT2G31370.7	66.027	365	93	10	1	361	1	338	3.42e-151	435
MsaG010824	AT2G31370.3	66.027	365	93	10	1	361	1	338	3.42e-151	435
MsaG010824	AT2G31370.6	66.027	365	93	10	1	361	1	338	3.42e-151	435
MsaG010824	AT2G40620.1	49.615	260	94	7	66	324	28	251	1.44e-61	204
MsaG010824	AT1G43700.1	49.746	197	90	3	161	348	129	325	2.69e-49	171
MsaG010824	AT1G06850.1	51.913	183	62	2	204	360	131	313	6.93e-48	167
MsaG010824	AT4G38900.2	67.769	121	39	0	199	319	372	492	9.73e-46	166
MsaG010824	AT4G38900.3	67.769	121	39	0	199	319	372	492	9.73e-46	166
MsaG010824	AT4G38900.1	64.567	127	39	1	199	319	372	498	2.57e-43	160
MsaG010824	AT2G21230.4	63.415	123	45	0	196	318	345	467	9.48e-43	157
MsaG010824	AT2G21230.1	63.415	123	45	0	196	318	345	467	9.48e-43	157
MsaG010824	AT2G21230.3	59.690	129	46	1	196	318	345	473	9.31e-40	149
MsaG010824	AT2G21230.2	65.421	107	37	0	196	302	345	451	4.68e-37	141
MsaG010824	AT1G06850.2	75.342	73	18	0	204	276	131	203	1.28e-26	107
MsaG010824	AT2G42380.2	47.000	100	53	0	219	318	184	283	7.82e-20	90.1
MsaG010824	AT3G58120.1	47.959	98	51	0	221	318	202	299	1.03e-19	90.1
MsaG010824	AT2G12900.1	34.109	129	77	1	196	316	83	211	9.62e-19	86.3
MsaG010824	AT2G42380.5	47.619	84	44	0	219	302	184	267	4.26e-17	81.3
MsaG010824	AT2G13150.1	37.615	109	67	1	207	315	136	243	4.26e-16	78.6
MsaG010824	AT3G58120.2	49.367	79	40	0	221	299	202	280	4.64e-16	78.6
MsaG010824	AT2G12940.1	34.188	117	76	1	197	313	125	240	3.04e-15	76.3
MsaG010824	AT2G42380.1	42.000	100	47	1	219	318	184	272	1.58e-13	71.6
MsaG010824	AT2G21235.1	43.678	87	49	0	221	307	359	445	3.28e-13	72.0
MsaG010824	AT1G58110.1	41.860	86	50	0	230	315	238	323	1.21e-11	66.2
MsaG010824	AT1G58110.2	41.860	86	50	0	230	315	238	323	1.21e-11	66.2
MsaG010824	AT2G42380.3	60.000	45	18	0	219	263	184	228	9.34e-11	62.0
MsaG010824	AT2G42380.4	60.000	45	18	0	219	263	184	228	9.34e-11	62.0

Find 119 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGTGCTGGTGTCTGATTC+TGG	0.087788	2:+78218204	None:intergenic
CCTATGAACTTTAATTCTTT+TGG	0.197858	2:-78215792	MsaT010824.1:CDS
GCTGTTTCTGAGGATGAATT+TGG	0.240472	2:+78215694	None:intergenic
ATCTGATGAAGGATTATCTT+TGG	0.251730	2:+78215524	None:intergenic
CCATGCCATGCACACTCTTT+TGG	0.268695	2:-78215739	MsaT010824.1:CDS
GTGCATGGCATGGTTATTAT+TGG	0.277488	2:+78215749	None:intergenic
TCAGGAGGAGGTACCTCTTC+TGG	0.279881	2:-78218262	MsaT010824.1:CDS
CTAGAATCTGAGCTGGTTCC+AGG	0.284646	2:+78218583	None:intergenic
TTCCGAGATTCTTACCTTGC+CGG	0.302193	2:-78218491	MsaT010824.1:CDS
ACCTCCATCAGGACGTTATC+CGG	0.323576	2:-78218677	MsaT010824.1:CDS
TACCTCTTCTGGGGAAAATA+TGG	0.331870	2:-78218251	MsaT010824.1:CDS
TCTGATGAAGGATTATCTTT+GGG	0.340833	2:+78215525	None:intergenic
CTGTGCTGGTGTCTGATTCT+GGG	0.348924	2:+78218205	None:intergenic
ACAAATGCAGCAGCAGCAGC+AGG	0.359774	2:-78215604	MsaT010824.1:CDS
TTTGTTGCTGCTGTATTTGT+TGG	0.370991	2:+78215637	None:intergenic
TGCACAGTTAACTCTCTTAC+AGG	0.371836	2:-78216510	MsaT010824.1:intron
CAGGAGGAGGTACCTCTTCT+GGG	0.381898	2:-78218261	MsaT010824.1:CDS
GTTGTCCATGTACCTTGATA+TGG	0.382193	2:-78218386	MsaT010824.1:CDS
CGCAGTTTCTCAACAATCCT+TGG	0.383849	2:+78215485	None:intergenic
TCGTATCCGCCGCTTGCTCC+TGG	0.385842	2:-78218601	MsaT010824.1:CDS
GTCTAATGCTGCCGGAGCAT+CGG	0.391354	2:-78218314	MsaT010824.1:CDS
TGCTGCCGGAGCATCGGGTT+TGG	0.393129	2:-78218308	MsaT010824.1:CDS
CATCCATGGACTGGCTGTGC+TGG	0.409055	2:+78218191	None:intergenic
GGAGCAACAAGTTCACTTGC+AGG	0.420631	2:-78216307	MsaT010824.1:CDS
TCTAATGCTGCCGGAGCATC+GGG	0.422071	2:-78218313	MsaT010824.1:CDS
CAAGCTTGCAGAACTTGCCT+TGG	0.423153	2:-78218083	MsaT010824.1:CDS
AATGGAAAAGGACAAATCAT+TGG	0.426956	2:-78218719	MsaT010824.1:CDS
CAAGGCAAGTTCTGCAAGCT+TGG	0.438060	2:+78218084	None:intergenic
TTGACCCCAAGCGTGCAAAA+AGG	0.442678	2:-78218060	MsaT010824.1:intron
TTTAAATGACGCGTTAAAAG+AGG	0.453730	2:-78215868	MsaT010824.1:CDS
TATCCGGGTTATTCACCAAC+TGG	0.455335	2:-78218661	MsaT010824.1:CDS
GTAACAGGATATGGGCAAAT+AGG	0.455791	2:-78216628	MsaT010824.1:intron
CAAGTGAACTTGTTGCTCCA+TGG	0.458049	2:+78216311	None:intergenic
TGCATGGCATGGTTATTATT+GGG	0.461227	2:+78215750	None:intergenic
ATTTGTGTGTAACAGGATAT+GGG	0.465089	2:-78216636	MsaT010824.1:intron
TGGCGCCAAACCCGATGCTC+CGG	0.468192	2:+78218303	None:intergenic
AAATCATTGGGTCTTGGCGG+TGG	0.469321	2:-78218706	MsaT010824.1:CDS
CAAACCTGAGATGTTAATCT+CGG	0.472947	2:-78218155	MsaT010824.1:CDS
TTCATCAGATGTAAATCCTT+CGG	0.478143	2:-78215511	MsaT010824.1:CDS
AAGTTCATAGGTCCTCCATT+TGG	0.480765	2:+78215804	None:intergenic
CCAAAAGAATTAAAGTTCAT+AGG	0.483391	2:+78215792	None:intergenic
CGTCTATGACCTCTATTTCT+CGG	0.485976	2:+78218520	None:intergenic
GCGGCGGATACGACGATGAT+GGG	0.489367	2:+78218611	None:intergenic
GGTTATTCACCAACTGGAAG+TGG	0.489501	2:-78218655	MsaT010824.1:CDS
AAGGACAAATCATTGGGTCT+TGG	0.496632	2:-78218712	MsaT010824.1:CDS
CAAGGAATCGTGAATGGAAA+AGG	0.498524	2:-78218731	None:intergenic
GGGCAAGCTATGCCGCCAAA+TGG	0.498892	2:-78215819	MsaT010824.1:CDS
GGTGTAGTTGGTGGTGCTGA+TGG	0.502800	2:-78218442	MsaT010824.1:CDS
GACAAATCATTGGGTCTTGG+CGG	0.504559	2:-78218709	MsaT010824.1:CDS
GAAGGATTTACATCTGATGA+AGG	0.504672	2:+78215513	None:intergenic
CGCATTTGAAAGTACTGACT+GGG	0.505823	2:-78215839	MsaT010824.1:CDS
ATGGAAAAGGACAAATCATT+GGG	0.507816	2:-78218718	MsaT010824.1:CDS
GATAGTGACCTCGGTGTAGT+TGG	0.507851	2:-78218454	MsaT010824.1:CDS
AGCGGCGGATACGACGATGA+TGG	0.508995	2:+78218610	None:intergenic
TTCATAGGTCCTCCATTTGG+CGG	0.509377	2:+78215807	None:intergenic
CAGCACAGCCAGTCCATGGA+TGG	0.510016	2:-78218190	MsaT010824.1:CDS
CAACAATCCTTGGCTGCCGA+AGG	0.516507	2:+78215495	None:intergenic
CTATCAAAGCTAATATCATC+CGG	0.517208	2:+78218472	None:intergenic
GATTGTTGTTGACCCATCCA+TGG	0.518412	2:+78218177	None:intergenic
CTTATCCATATCAAGGTACA+TGG	0.520173	2:+78218381	None:intergenic
ATGTCGGTAGGGCAGACATC+AGG	0.524354	2:-78218280	MsaT010824.1:CDS
GCTATATTGCTGAGCTTGAA+AGG	0.525278	2:-78216571	MsaT010824.1:CDS
CCTCCATCAGGACGTTATCC+GGG	0.528655	2:-78218676	MsaT010824.1:CDS
ATCGGGTTTGGCGCCAATGT+CGG	0.530842	2:-78218296	MsaT010824.1:CDS
GGTTTGGCGCCAATGTCGGT+AGG	0.535811	2:-78218292	MsaT010824.1:CDS
GATGTCTGCCCTACCGACAT+TGG	0.538923	2:+78218283	None:intergenic
ATCCGGCAAGGTAAGAATCT+CGG	0.540794	2:+78218489	None:intergenic
CTGCGGCAAGGTCAAAAGAA+AGG	0.542300	2:-78216601	MsaT010824.1:CDS
CATGGACAACAAATCTTCCT+CGG	0.543636	2:+78218399	None:intergenic
GAAGGATTATCTTTGGGGCA+TGG	0.547961	2:+78215531	None:intergenic
AATTGAACTTATCCATATCA+AGG	0.549561	2:+78218374	None:intergenic
TGTAAATCCTTCGGCAGCCA+AGG	0.553319	2:-78215502	MsaT010824.1:CDS
GAACCCGAGATTAACATCTC+AGG	0.557523	2:+78218151	None:intergenic
ACGCATTTGAAAGTACTGAC+TGG	0.558522	2:-78215840	MsaT010824.1:CDS
ACACCAGCACAGCCAGTCCA+TGG	0.559692	2:-78218194	MsaT010824.1:CDS
GGTGAGGGGTCTAATGCTGC+CGG	0.563134	2:-78218322	MsaT010824.1:CDS
TTGTTGACCCATCCATGGAC+TGG	0.565600	2:+78218182	None:intergenic
GAAAGTGCAGACATTGCAAA+CGG	0.566063	2:-78216549	MsaT010824.1:CDS
AAACCTGAGATGTTAATCTC+GGG	0.567313	2:-78218154	MsaT010824.1:CDS
GATAATCCTCCGAGAAATAG+AGG	0.571302	2:-78218529	MsaT010824.1:CDS
TGCACGCTTGGGGTCAACCA+AGG	0.572453	2:+78218066	None:intergenic
TGATGTTGCTGCTGTTTCTG+AGG	0.574585	2:+78215684	None:intergenic
CAAGCTATGCCGCCAAATGG+AGG	0.574883	2:-78215816	MsaT010824.1:CDS
CTGATGAAGGATTATCTTTG+GGG	0.574967	2:+78215526	None:intergenic
CGGCGGATACGACGATGATG+GGG	0.587072	2:+78218612	None:intergenic
AAAGCCACTAGAATCTGAGC+TGG	0.591242	2:+78218576	None:intergenic
CCAAAAGAGTGTGCATGGCA+TGG	0.592655	2:+78215739	None:intergenic
CTTCCAGTTGGTGAATAACC+CGG	0.596092	2:+78218658	None:intergenic
GGATAACGTCCTGATGGAGG+TGG	0.599011	2:+78218679	None:intergenic
ATTAGCTTTGATAGTGACCT+CGG	0.599687	2:-78218463	MsaT010824.1:CDS
ACATTGAAACCACTTCCAGT+TGG	0.606876	2:+78218646	None:intergenic
TGCAGCCAAAAGAGTGTGCA+TGG	0.611169	2:+78215734	None:intergenic
AGTGACCTCGGTGTAGTTGG+TGG	0.612819	2:-78218451	MsaT010824.1:CDS
GTTTGGCGCCAATGTCGGTA+GGG	0.616468	2:-78218291	MsaT010824.1:CDS
AGCACAGCCAGTCCATGGAT+GGG	0.619422	2:-78218189	MsaT010824.1:CDS
GAGCTGGTTCCAGGAGCAAG+CGG	0.619940	2:+78218592	None:intergenic
GAAGCTGCGGTTGCAAACCA+TGG	0.620439	2:-78216328	MsaT010824.1:CDS
CAACAAGTTCACTTGCAGGA+TGG	0.622286	2:-78216303	MsaT010824.1:intron
TAACCCGGATAACGTCCTGA+TGG	0.622648	2:+78218673	None:intergenic
CTGGTTCCAGGAGCAAGCGG+CGG	0.624878	2:+78218595	None:intergenic
AAAGCTAATATCATCCGGCA+AGG	0.625947	2:+78218477	None:intergenic
CAAATAGGCAGTCTGCGGCA+AGG	0.630192	2:-78216613	MsaT010824.1:CDS
TCTGGGGAAAATATGGCAGT+CGG	0.630934	2:-78218244	MsaT010824.1:CDS
CCCGGATAACGTCCTGATGG+AGG	0.636399	2:+78218676	None:intergenic
TCGGTAGGGCAGACATCAGG+AGG	0.636796	2:-78218277	MsaT010824.1:CDS
CAGTCGGTACTACAAATGAA+AGG	0.639281	2:-78218228	MsaT010824.1:CDS
GGAACCAGCTCAGATTCTAG+TGG	0.650014	2:-78218580	MsaT010824.1:CDS
GGATTATCTTTGGGGCATGG+AGG	0.651440	2:+78215534	None:intergenic
AGGAGGAGGTACCTCTTCTG+GGG	0.666286	2:-78218260	MsaT010824.1:CDS
ATGAACAGAGAGATACAAGT+GGG	0.666459	2:-78216374	MsaT010824.1:intron
GTAGGGCAGACATCAGGAGG+AGG	0.666831	2:-78218274	MsaT010824.1:CDS
CTATGACCTCTATTTCTCGG+AGG	0.675680	2:+78218523	None:intergenic
ATGGGCAAATAGGCAGTCTG+CGG	0.682103	2:-78216618	MsaT010824.1:CDS
AGAACAGTGAGCTGAAGCTG+CGG	0.689015	2:-78216341	MsaT010824.1:CDS
GTCGTTCTCTGATGATACCG+AGG	0.690738	2:-78218416	MsaT010824.1:CDS
TATGAACAGAGAGATACAAG+TGG	0.694542	2:-78216375	MsaT010824.1:intron
GGCGGATACGACGATGATGG+GGG	0.706397	2:+78218613	None:intergenic
TCGGTATCATCAGAGAACGA+CGG	0.752661	2:+78218418	None:intergenic
CAGCACCACCAACTACACCG+AGG	0.826194	2:+78218446	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	78215494	78218740	78215494	ID=MsaG010824
Chr2	mRNA	78215494	78218740	78215494	ID=MsaT010824.1;Parent=MsaG010824
Chr2	exon	78215494	78215891	78215494	ID=MsaT010824.1.exon4;Parent=MsaT010824.1
Chr2	CDS	78215494	78215891	78215494	ID=cds.MsaT010824.1;Parent=MsaT010824.1
Chr2	exon	78216304	78216388	78216304	ID=MsaT010824.1.exon3;Parent=MsaT010824.1
Chr2	CDS	78216304	78216388	78216304	ID=cds.MsaT010824.1;Parent=MsaT010824.1
Chr2	exon	78216511	78216643	78216511	ID=MsaT010824.1.exon2;Parent=MsaT010824.1
Chr2	CDS	78216511	78216643	78216511	ID=cds.MsaT010824.1;Parent=MsaT010824.1
Chr2	exon	78218061	78218740	78218061	ID=MsaT010824.1.exon1;Parent=MsaT010824.1
Chr2	CDS	78218061	78218740	78218061	ID=cds.MsaT010824.1;Parent=MsaT010824.1

Gene Sequence

>MsaT010824.1

ATGGAAAAGGACAAATCATTGGGTCTTGGCGGTGGTTTTCCACCTCCATCAGGACGTTATCCGGGTTATTCACCAACTGGAAGTGGTTTCAATGTGAAATCTGAACCCCCATCATCGTCGTATCCGCCGCTTGCTCCTGGAACCAGCTCAGATTCTAGTGGCTTTAGTCATGATATAAGCAAAATGTCTGATAATCCTCCGAGAAATAGAGGTCATAGACGTGCTCATTCCGAGATTCTTACCTTGCCGGATGATATTAGCTTTGATAGTGACCTCGGTGTAGTTGGTGGTGCTGATGGACCGTCGTTCTCTGATGATACCGAGGAAGATTTGTTGTCCATGTACCTTGATATGGATAAGTTCAATTCGTCGTCAGCAACGTCTAATTTTCAAATGGGTGAGGGGTCTAATGCTGCCGGAGCATCGGGTTTGGCGCCAATGTCGGTAGGGCAGACATCAGGAGGAGGTACCTCTTCTGGGGAAAATATGGCAGTCGGTACTACAAATGAAAGGCCCAGAATCAGACACCAGCACAGCCAGTCCATGGATGGGTCAACAACAATCAAACCTGAGATGTTAATCTCGGGTTCAGATGACATGTCTGCAGCCGATTCCAAAAAAGCAATGTCAGCAGCCAAGCTTGCAGAACTTGCCTTGGTTGACCCCAAGCGTGCAAAAAGGATATGGGCAAATAGGCAGTCTGCGGCAAGGTCAAAAGAAAGGAAGATGCGCTATATTGCTGAGCTTGAAAGGAAAGTGCAGACATTGCAAACGGAAGCAACATCTTTGTCTGCACAGTTAACTCTCTTACAGAGAGATACAAGTGGGCTGAATTCTGAGAACAGTGAGCTGAAGCTGCGGTTGCAAACCATGGAGCAACAAGTTCACTTGCAGGATGCTTTAAATGACGCGTTAAAAGAGGAAATTACGCATTTGAAAGTACTGACTGGGCAAGCTATGCCGCCAAATGGAGGACCTATGAACTTTAATTCTTTTGGAGGAGGGCAGCAGTTCTATCCCAATAATAACCATGCCATGCACACTCTTTTGGCTGCACAACAGTTTCAACAGCTCCAAATTCATCCTCAGAAACAGCAGCAACATCAGTTTCAGCAACAAATACAACAACACCAACAAATACAGCAGCAACAAATGCAACAGCAACAAATGCAGCAGCAGCAGCAGGAACAACAACAACAACAATCAGGTGACTTGAAAATGAGAGCAACTACACCTCCATGCCCCAAAGATAATCCTTCATCAGATGTAAATCCTTCGGCAGCCAAGGATTGTTGA

Protein sequence

>MsaT010824.1

MEKDKSLGLGGGFPPPSGRYPGYSPTGSGFNVKSEPPSSSYPPLAPGTSSDSSGFSHDISKMSDNPPRNRGHRRAHSEILTLPDDISFDSDLGVVGGADGPSFSDDTEEDLLSMYLDMDKFNSSSATSNFQMGEGSNAAGASGLAPMSVGQTSGGGTSSGENMAVGTTNERPRIRHQHSQSMDGSTTIKPEMLISGSDDMSAADSKKAMSAAKLAELALVDPKRAKRIWANRQSAARSKERKMRYIAELERKVQTLQTEATSLSAQLTLLQRDTSGLNSENSELKLRLQTMEQQVHLQDALNDALKEEITHLKVLTGQAMPPNGGPMNFNSFGGGQQFYPNNNHAMHTLLAAQQFQQLQIHPQKQQQHQFQQQIQQHQQIQQQQMQQQQMQQQQQEQQQQQSGDLKMRATTPPCPKDNPSSDVNPSAAKDC*