AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010832

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010832 MtrunA17_Chr2g0329631 98.340 241 4 0 1 241 1 241 2.41e-178 489
MsaG010832 MtrunA17_Chr1g0163301 45.178 197 100 4 39 232 89 280 4.48e-49 164
MsaG010832 MtrunA17_Chr4g0037581 48.148 81 39 1 39 119 89 166 1.38e-20 85.5
MsaG010832 MtrunA17_Chr4g0037591 43.243 74 42 0 135 208 22 95 3.41e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010832 AT3G07565.1 64.286 196 68 2 39 233 56 250 2.13e-91 270
MsaG010832 AT3G07565.3 63.959 197 68 3 39 233 56 251 1.22e-89 265
MsaG010832 AT3G07565.4 61.165 206 68 4 39 233 56 260 1.02e-86 258
MsaG010832 AT3G07565.2 62.810 121 43 2 39 158 56 175 1.62e-49 161
MsaG010832 AT1G60670.2 36.818 220 122 5 23 232 19 231 4.78e-41 141
MsaG010832 AT1G60670.5 36.818 220 122 5 23 232 19 231 5.38e-41 141
MsaG010832 AT1G10820.1 37.500 208 120 4 30 235 13 212 5.59e-41 140
MsaG010832 AT1G10820.2 37.019 208 121 3 30 235 39 238 6.30e-41 141
MsaG010832 AT1G60670.3 36.364 220 118 5 23 232 19 226 4.49e-40 139
MsaG010832 AT1G60670.4 36.735 196 107 5 23 208 19 207 3.80e-32 117
MsaG010832 AT1G60670.1 36.686 169 90 5 23 181 19 180 2.01e-25 99.4
MsaG010832 AT1G68160.1 34.197 193 119 3 39 228 70 257 4.17e-23 95.1
MsaG010832 AT2G43470.1 31.818 220 124 7 22 236 6 204 3.09e-21 88.6

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAAAGGTTTGCAGAGATT+TGG 0.261482 2:+78344292 None:intergenic
TATAGAGATTAACCTGAATA+AGG 0.265978 2:+78344564 None:intergenic
TCGAGAATGGATTGTTCTTC+AGG 0.267802 2:+78350333 None:intergenic
GATGATGGCATCCCTTATTC+AGG 0.308846 2:-78344576 MsaT010832.1:intron
GGAAAGGTTTGCAGAGATTT+GGG 0.332027 2:+78344293 None:intergenic
TAGGTTATGGCGAACCCTTC+TGG 0.336467 2:-78350489 None:intergenic
ACAAGACCTGAAGAGGTTTC+AGG 0.340002 2:+78350399 None:intergenic
GAGAGGTGTTCCTGGGAGTA+TGG 0.344621 2:+78343268 None:intergenic
TCCGTTCCTGAAACCTCTTC+AGG 0.347567 2:-78350405 MsaT010832.1:CDS
TGCGCCACCAATGATTACTA+TGG 0.371818 2:-78344604 MsaT010832.1:CDS
TGATGATGTTGTCCCTAGTT+TGG 0.384182 2:+78343597 None:intergenic
GTAATCATTGGTGGCGCATA+TGG 0.406796 2:+78344609 None:intergenic
CGAGAATGGATTGTTCTTCA+GGG 0.417587 2:+78350334 None:intergenic
TGAGGATGGAGAGGTGTTCC+TGG 0.420236 2:+78343260 None:intergenic
ATGGCGAACCCTTCTGGTAC+TGG 0.425393 2:-78350483 MsaT010832.1:CDS
TGCAAACCTTTCCTCTCTCC+AGG 0.433697 2:-78344283 MsaT010832.1:intron
TGTCCCTAGTTTGGCACAAA+AGG 0.448804 2:+78343606 None:intergenic
AGCTCTTCATTCATCTTCAC+TGG 0.451576 2:+78343299 None:intergenic
GAACAATCCATTCTCGAAGA+TGG 0.453682 2:-78350327 MsaT010832.1:CDS
GGCGAGTCCATCTTCGAGAA+TGG 0.457858 2:+78350320 None:intergenic
AGCTAAATAACAAGACAGTC+CGG 0.470131 2:-78347558 MsaT010832.1:CDS
TCATTCATCTTCACTGGAAG+AGG 0.470717 2:+78343305 None:intergenic
GGCATTTGCTTCATCACCGC+TGG 0.470801 2:+78343329 None:intergenic
ATCCTGGCATATCTCTTGAT+TGG 0.473143 2:-78350359 MsaT010832.1:CDS
GGGATGTGGCACTACGTGTC+AGG 0.479331 2:-78347537 MsaT010832.1:CDS
TGGCGCATATGGAGGGACAC+TGG 0.491577 2:+78344620 None:intergenic
GGCGCATATGGAGGGACACT+GGG 0.494739 2:+78344621 None:intergenic
ATAGAGATTAACCTGAATAA+GGG 0.495423 2:+78344565 None:intergenic
AGAAAATAGCAAGAGAAGGA+AGG 0.497749 2:-78345730 MsaT010832.1:CDS
GTTTGCAGAGATTTGGGTCA+TGG 0.508018 2:+78344299 None:intergenic
TCATTGTCCATAGTAATCAT+TGG 0.509412 2:+78344597 None:intergenic
TGTTCCTGGGAGTATGGAAC+GGG 0.524742 2:+78343274 None:intergenic
GAATTGCCGTTAAAAGAAGA+AGG 0.528190 2:+78350426 None:intergenic
ATCATTGGTGGCGCATATGG+AGG 0.535761 2:+78344612 None:intergenic
GTTCATGACAAGACCTGAAG+AGG 0.548014 2:+78350392 None:intergenic
TTCATCTTCACTGGAAGAGG+CGG 0.554591 2:+78343308 None:intergenic
AGAAAGAAAATAGCAAGAGA+AGG 0.556600 2:-78345734 MsaT010832.1:CDS
ATGAACATGAAGCATAATCC+TGG 0.559962 2:-78350375 MsaT010832.1:CDS
GAGGATGGAGAGGTGTTCCT+GGG 0.560302 2:+78343261 None:intergenic
TAACAAGACAGTCCGGGATG+TGG 0.565990 2:-78347551 MsaT010832.1:CDS
ATTACTATGGACAATGATGA+TGG 0.578059 2:-78344591 MsaT010832.1:CDS
ACCTGAAGAGGTTTCAGGAA+CGG 0.587306 2:+78350404 None:intergenic
CTGTCCCGTTCCATACTCCC+AGG 0.588528 2:-78343278 MsaT010832.1:CDS
GAGAATGGATTGTTCTTCAG+GGG 0.594056 2:+78350335 None:intergenic
TCATCAAGAACAACATACTC+AGG 0.599029 2:-78350454 MsaT010832.1:CDS
TCATTGGTGGCGCATATGGA+GGG 0.599262 2:+78344613 None:intergenic
GCTAAATAACAAGACAGTCC+GGG 0.602112 2:-78347557 MsaT010832.1:CDS
GTGTTCCTGGGAGTATGGAA+CGG 0.607968 2:+78343273 None:intergenic
ACGCACGATGTTAGATTCTG+AGG 0.609319 2:+78347600 None:intergenic
TTGTCCATAGTAATCATTGG+TGG 0.612471 2:+78344600 None:intergenic
GACCAATCAAGAGATATGCC+AGG 0.613497 2:+78350357 None:intergenic
CTGCTCTAATAGCTCAGCAG+TGG 0.615347 2:+78344332 None:intergenic
TGCTCTAATAGCTCAGCAGT+GGG 0.617093 2:+78344333 None:intergenic
CATCATGACTGAGGATGGAG+AGG 0.625694 2:+78343251 None:intergenic
TGACACGTAGTGCCACATCC+CGG 0.630734 2:+78347539 None:intergenic
TGATTACCACCAGTACCAGA+AGG 0.632355 2:+78350474 None:intergenic
TTGTCAACACACCTGGAGAG+AGG 0.651066 2:+78344272 None:intergenic
ATGTGGCACTACGTGTCAGG+TGG 0.654900 2:-78347534 MsaT010832.1:CDS
GCGAACCCTTCTGGTACTGG+TGG 0.657804 2:-78350480 MsaT010832.1:CDS
GATTACCACCAGTACCAGAA+GGG 0.663106 2:+78350475 None:intergenic
AACACACCTGGAGAGAGGAA+AGG 0.663481 2:+78344277 None:intergenic
CAGGATGAATGATTCACCAG+CGG 0.722993 2:-78343345 MsaT010832.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 78343253 78350505 78343253 ID=MsaG010832
Chr2 mRNA 78343253 78350505 78343253 ID=MsaT010832.1;Parent=MsaG010832
Chr2 exon 78343253 78343364 78343253 ID=MsaT010832.1.exon7;Parent=MsaT010832.1
Chr2 CDS 78343253 78343364 78343253 ID=cds.MsaT010832.1;Parent=MsaT010832.1
Chr2 exon 78343584 78343645 78343584 ID=MsaT010832.1.exon6;Parent=MsaT010832.1
Chr2 CDS 78343584 78343645 78343584 ID=cds.MsaT010832.1;Parent=MsaT010832.1
Chr2 exon 78344284 78344366 78344284 ID=MsaT010832.1.exon5;Parent=MsaT010832.1
Chr2 CDS 78344284 78344366 78344284 ID=cds.MsaT010832.1;Parent=MsaT010832.1
Chr2 exon 78344577 78344691 78344577 ID=MsaT010832.1.exon4;Parent=MsaT010832.1
Chr2 CDS 78344577 78344691 78344577 ID=cds.MsaT010832.1;Parent=MsaT010832.1
Chr2 exon 78345692 78345757 78345692 ID=MsaT010832.1.exon3;Parent=MsaT010832.1
Chr2 CDS 78345692 78345757 78345692 ID=cds.MsaT010832.1;Parent=MsaT010832.1
Chr2 exon 78347528 78347627 78347528 ID=MsaT010832.1.exon2;Parent=MsaT010832.1
Chr2 CDS 78347528 78347627 78347528 ID=cds.MsaT010832.1;Parent=MsaT010832.1
Chr2 exon 78350318 78350505 78350318 ID=MsaT010832.1.exon1;Parent=MsaT010832.1
Chr2 CDS 78350318 78350505 78350318 ID=cds.MsaT010832.1;Parent=MsaT010832.1
Gene Sequence

>MsaT010832.1

ATGGCGAACCCTTCTGGTACTGGTGGTAATCATCAAGAACAACATACTCAGGTGAATCCTTCTTCTTTTAACGGCAATTCCGTTCCTGAAACCTCTTCAGGTCTTGTCATGAACATGAAGCATAATCCTGGCATATCTCTTGATTGGTCCCCTGAAGAACAATCCATTCTCGAAGATGGACTCGCCAAGTATGCCTCAGAATCTAACATCGTGCGTTATGCAAAGATAGCTCAACAGCTAAATAACAAGACAGTCCGGGATGTGGCACTACGTGTCAGGTGGATGAACAAGAAAGAAAATAGCAAGAGAAGGAAGGATGATCACAATTTGTCACGAAAAAGTAAAGATAAAAAGGAAAGGGTTTCTGATCCTGCAGCAAAATCATCTCATTTTGCTGCTCGACCCAGTGTCCCTCCATATGCGCCACCAATGATTACTATGGACAATGATGATGGCATCCCTTATTCAGCAATTGGAGGTCCCACTGCTGAGCTATTAGAGCAGAATGCACAGACCATGACCCAAATCTCTGCAAACCTTTCCTCTCTCCAGTTACAGGAAAACATCAACCTTTTGTGCCAAACTAGGGACAACATCATCAGAATTATGAATGAGATGAATGATTCACCAGCGGTGATGAAGCAAATGCCGCCTCTTCCAGTGAAGATGAATGAAGAGCTGTCCCGTTCCATACTCCCAGGAACACCTCTCCATCCTCAGTCATGA
Protein sequence

>MsaT010832.1

MANPSGTGGNHQEQHTQVNPSSFNGNSVPETSSGLVMNMKHNPGISLDWSPEEQSILEDGLAKYASESNIVRYAKIAQQLNNKTVRDVALRVRWMNKKENSKRRKDDHNLSRKSKDKKERVSDPAAKSSHFAARPSVPPYAPPMITMDNDDGIPYSAIGGPTAELLEQNAQTMTQISANLSSLQLQENINLLCQTRDNIIRIMNEMNDSPAVMKQMPPLPVKMNEELSRSILPGTPLHPQS*