AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010857

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010857 MtrunA17_Chr2g0329821 99.600 250 1 0 1 250 1 250 0.0 514
MsaG010857 MtrunA17_Chr8g0343441 80.081 246 48 1 6 250 10 255 1.12e-123 352
MsaG010857 MtrunA17_Chr2g0300241 72.177 248 58 5 6 250 13 252 1.13e-113 326
MsaG010857 MtrunA17_Chr4g0076771 67.857 252 76 1 1 247 1 252 1.37e-111 321
MsaG010857 MtrunA17_Chr1g0150821 68.775 253 70 3 6 250 11 262 2.02e-108 313
MsaG010857 MtrunA17_Chr4g0006381 61.600 250 85 5 1 250 3 241 1.49e-91 270
MsaG010857 MtrunA17_Chr7g0242511 58.607 244 91 4 1 244 3 236 2.30e-86 256
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010857 AT3G42790.1 72.653 245 61 3 6 250 11 249 2.46e-118 338
MsaG010857 AT5G20510.1 69.200 250 71 2 6 250 11 259 1.37e-113 327
MsaG010857 AT1G14510.1 67.755 245 76 3 6 250 10 251 7.18e-106 306
MsaG010857 AT2G02470.1 68.340 259 69 6 1 250 1 255 6.79e-105 304
MsaG010857 AT2G02470.2 68.504 254 68 6 1 250 1 246 4.83e-103 299
MsaG010857 AT5G26210.1 70.732 246 70 2 6 250 10 254 1.98e-93 275
MsaG010857 AT5G20510.2 68.519 216 62 2 40 250 4 218 5.28e-92 270
MsaG010857 AT5G05610.2 60.331 242 84 5 6 247 9 238 3.61e-88 261
MsaG010857 AT5G05610.1 60.331 242 84 5 6 247 9 238 3.61e-88 261
MsaG010857 AT3G11200.1 61.570 242 84 5 6 247 11 243 9.78e-86 255
MsaG010857 AT5G20510.3 74.194 155 40 0 6 160 11 165 1.02e-75 228
MsaG010857 AT3G11200.2 61.538 208 71 5 40 247 32 230 4.83e-72 220
MsaG010857 AT2G02470.3 72.785 158 38 2 1 154 1 157 1.04e-66 207

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCTTCCTCCTCATCTTT+TGG 0.240666 2:-78575554 None:intergenic
GGTTGTCTGCTCTGAGCTTC+AGG 0.243347 2:-78575515 None:intergenic
TTCTACTTTGGAGCCAGATT+TGG 0.290182 2:+78573435 MsaT010857.1:CDS
ACGGATTGCTTAATGAGAAT+TGG 0.301755 2:+78573268 MsaT010857.1:CDS
AAGGAGAATTTGTGCTTATA+CGG 0.310260 2:+78573249 MsaT010857.1:CDS
AACAGGGCGAAGCCACTTGC+GGG 0.339120 2:+78575597 MsaT010857.1:CDS
TTTGGCTGTAATGCCGCTTT+TGG 0.349429 2:-78575536 None:intergenic
GTAACGGGGCTGATGAGTTC+TGG 0.350703 2:+78575633 MsaT010857.1:CDS
TGCTGGGTATCAACTTTGCC+AGG 0.351612 2:+78573340 MsaT010857.1:CDS
CTTTGATGATTCCAGTTCTT+CGG 0.353581 2:-78572436 None:intergenic
AAGAAATTATACCGACGGTT+AGG 0.356361 2:-78572460 None:intergenic
ATTATTTACCGCTTTAGAAC+TGG 0.360765 2:-78574261 None:intergenic
GGGCTTGTGGTGATAGTAAC+GGG 0.362851 2:+78575618 MsaT010857.1:CDS
AGAGATTCCTGAGCCTGTGC+TGG 0.371017 2:+78573323 MsaT010857.1:CDS
GATTTCAAGAGCCGAAGAAC+TGG 0.371977 2:+78572425 MsaT010857.1:CDS
ACAGTATTTGAAGTTGTAAC+TGG 0.378246 2:+78574173 MsaT010857.1:CDS
CTTTCCCTTGCATTCTACTT+TGG 0.386606 2:+78573423 MsaT010857.1:CDS
CGGATTGCTTAATGAGAATT+GGG 0.394937 2:+78573269 MsaT010857.1:CDS
TTTCTCTTGCATTCCATCCC+TGG 0.397443 2:-78573358 None:intergenic
AGAGCAGGATGATGATGAAC+AGG 0.397479 2:+78575580 MsaT010857.1:CDS
AGCTTTATCAAATCCAAATC+TGG 0.410286 2:-78573448 None:intergenic
AGCACAGGCTCAGGAATCTC+TGG 0.425752 2:-78573321 None:intergenic
AAAAGATGAGGAGGAAGAAC+TGG 0.426868 2:+78575556 MsaT010857.1:CDS
TCTTGTTACTGCTGCATGAT+GGG 0.433135 2:-78575745 None:intergenic
TCTGGAGGAACTTCTTCAAC+AGG 0.445499 2:-78573303 None:intergenic
AGAAATTATACCGACGGTTA+GGG 0.452362 2:-78572459 None:intergenic
GGAGGAAGAACTGGAAGAGC+AGG 0.470600 2:+78575565 MsaT010857.1:CDS
CTCTTGTTACTGCTGCATGA+TGG 0.480424 2:-78575746 None:intergenic
TTCTACAGACAATGTGATCC+TGG 0.488698 2:+78572646 MsaT010857.1:CDS
TTGATACCCAGCACAGGCTC+AGG 0.497706 2:-78573330 None:intergenic
ATCACTGTGAACAGCAACTA+AGG 0.498800 2:-78573391 None:intergenic
TTGCTGTTCACAGTGATACA+TGG 0.507689 2:+78573397 MsaT010857.1:CDS
ATCTGCGAGAAGTGGTTCCA+TGG 0.517485 2:+78575668 MsaT010857.1:CDS
GGTATCAACTTTGCCAGGGA+TGG 0.520071 2:+78573345 MsaT010857.1:CDS
GAGATTCCTGAGCCTGTGCT+GGG 0.523852 2:+78573324 MsaT010857.1:CDS
ATGGAATGCAAGAGAAAGAC+TGG 0.533268 2:+78573364 MsaT010857.1:CDS
GAAGCCACTTGCGGGGCTTG+TGG 0.543776 2:+78575605 MsaT010857.1:CDS
TTGATATGCTCAGCACGAGC+AGG 0.552258 2:-78575710 None:intergenic
GAATCGAATTGAACAATGGA+AGG 0.552287 2:+78572371 None:intergenic
TTGGATTTGATAAAGCTGAC+AGG 0.552742 2:+78573454 MsaT010857.1:CDS
GGGGCTTGTGGTGATAGTAA+CGG 0.558893 2:+78575617 MsaT010857.1:CDS
GTTACAACTTCAAATACTGT+TGG 0.565683 2:-78574170 None:intergenic
GCTGCGACATCTGCGAGAAG+TGG 0.566172 2:+78575660 MsaT010857.1:CDS
GAACAGGGCGAAGCCACTTG+CGG 0.571946 2:+78575596 MsaT010857.1:CDS
TGGCTCCAAAGTAGAATGCA+AGG 0.572860 2:-78573428 None:intergenic
ATCTAAGTCCAGTTCTAAAG+CGG 0.587642 2:+78574253 MsaT010857.1:CDS
ATCACCACAAGCCCCGCAAG+TGG 0.598002 2:-78575609 None:intergenic
TCAGAGCAGACAACCAAAAG+CGG 0.606960 2:+78575523 MsaT010857.1:CDS
GCTGGGTATCAACTTTGCCA+GGG 0.620748 2:+78573341 MsaT010857.1:CDS
GCAAAGTTGATACCCAGCAC+AGG 0.629760 2:-78573336 None:intergenic
ATCATCAAAGCCCTAACCGT+CGG 0.634254 2:+78572449 MsaT010857.1:CDS
GGCATTACAGCCAAAAGATG+AGG 0.636479 2:+78575544 MsaT010857.1:CDS
GGCTCCAAAGTAGAATGCAA+GGG 0.639786 2:-78573427 None:intergenic
ACAGGGCGAAGCCACTTGCG+GGG 0.643480 2:+78575598 MsaT010857.1:CDS
ACAGGCTCAGGAATCTCTGG+AGG 0.651339 2:-78573318 None:intergenic
ATTACAGCCAAAAGATGAGG+AGG 0.661732 2:+78575547 MsaT010857.1:CDS
GAGCAGGATGATGATGAACA+GGG 0.682274 2:+78575581 MsaT010857.1:CDS
GGCTTGTGGTGATAGTAACG+GGG 0.707309 2:+78575619 MsaT010857.1:CDS
TCTTCAGTCTCAAACAACAG+TGG 0.724521 2:+78574224 MsaT010857.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 78572386 78575778 78572386 ID=MsaG010857
Chr2 mRNA 78572386 78575778 78572386 ID=MsaT010857.1;Parent=MsaG010857
Chr2 exon 78572386 78572470 78572386 ID=MsaT010857.1.exon1;Parent=MsaT010857.1
Chr2 CDS 78572386 78572470 78572386 ID=cds.MsaT010857.1;Parent=MsaT010857.1
Chr2 exon 78572635 78572667 78572635 ID=MsaT010857.1.exon2;Parent=MsaT010857.1
Chr2 CDS 78572635 78572667 78572635 ID=cds.MsaT010857.1;Parent=MsaT010857.1
Chr2 exon 78573247 78573475 78573247 ID=MsaT010857.1.exon3;Parent=MsaT010857.1
Chr2 CDS 78573247 78573475 78573247 ID=cds.MsaT010857.1;Parent=MsaT010857.1
Chr2 exon 78574139 78574274 78574139 ID=MsaT010857.1.exon4;Parent=MsaT010857.1
Chr2 CDS 78574139 78574274 78574139 ID=cds.MsaT010857.1;Parent=MsaT010857.1
Chr2 exon 78575509 78575778 78575509 ID=MsaT010857.1.exon5;Parent=MsaT010857.1
Chr2 CDS 78575509 78575778 78575509 ID=cds.MsaT010857.1;Parent=MsaT010857.1
Gene Sequence

>MsaT010857.1

ATGGAAGGTCGAAGTCGCACCGTTGAACAAATTTTTGATGATTTCAAGAGCCGAAGAACTGGAATCATCAAAGCCCTAACCGTCGATGTTGAAGATTTCTACAGACAATGTGATCCTGAAAAGGAGAATTTGTGCTTATACGGATTGCTTAATGAGAATTGGGAAGTGAATTTACCTGTTGAAGAAGTTCCTCCAGAGATTCCTGAGCCTGTGCTGGGTATCAACTTTGCCAGGGATGGAATGCAAGAGAAAGACTGGTTAGCCTTAGTTGCTGTTCACAGTGATACATGGTTACTTTCCCTTGCATTCTACTTTGGAGCCAGATTTGGATTTGATAAAGCTGACAGGAAACGTCTATTCAATTTGATCAATGAACTGCCAACAGTATTTGAAGTTGTAACTGGTGCTGCAAAGAAACAAGTCAAAGAGAAGTCTTCAGTCTCAAACAACAGTGGCAGCAAATCTAAGTCCAGTTCTAAAGCGCGAGCTCCTGAAGCTCAGAGCAGACAACCAAAAGCGGCATTACAGCCAAAAGATGAGGAGGAAGAACTGGAAGAGCAGGATGATGATGAACAGGGCGAAGCCACTTGCGGGGCTTGTGGTGATAGTAACGGGGCTGATGAGTTCTGGATTTGCTGCGACATCTGCGAGAAGTGGTTCCATGGAAAATGTGTGAAGATCACTCCTGCTCGTGCTGAGCATATCAAGCAATACAAGTGCCCATCATGCAGCAGTAACAAGAGAGCTCGCTAG
Protein sequence

>MsaT010857.1

MEGRSRTVEQIFDDFKSRRTGIIKALTVDVEDFYRQCDPEKENLCLYGLLNENWEVNLPVEEVPPEIPEPVLGINFARDGMQEKDWLALVAVHSDTWLLSLAFYFGARFGFDKADRKRLFNLINELPTVFEVVTGAAKKQVKEKSSVSNNSGSKSKSSSKARAPEAQSRQPKAALQPKDEEEELEEQDDDEQGEATCGACGDSNGADEFWICCDICEKWFHGKCVKITPARAEHIKQYKCPSCSSNKRAR*