AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010910 MtrunA17_Chr8g0347741 54.403 318 41 6 2 231 43 344 2.97e-91 275
MsaG010910 MtrunA17_Chr7g0275321 50.781 256 64 6 22 231 106 345 8.21e-62 199
MsaG010910 MtrunA17_Chr8g0369161 58.904 73 28 1 159 231 301 371 1.05e-19 86.7
MsaG010910 MtrunA17_Chr8g0373571 60.526 76 27 2 157 231 251 324 1.67e-19 85.9
MsaG010910 MtrunA17_Chr5g0410531 60.274 73 27 1 159 231 302 372 6.19e-19 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010910 AT2G46270.1 37.539 317 78 10 2 230 38 322 1.87e-40 143
MsaG010910 AT2G46270.3 37.539 317 78 10 2 230 38 322 1.87e-40 143
MsaG010910 AT2G46270.2 35.016 317 63 9 2 230 38 299 8.93e-31 117
MsaG010910 AT2G46270.4 35.016 317 63 9 2 230 38 299 8.93e-31 117
MsaG010910 AT4G01120.1 31.715 309 96 10 2 230 39 312 1.47e-25 102
MsaG010910 AT2G35530.1 36.449 214 106 9 38 231 166 369 2.90e-21 91.7
MsaG010910 AT1G32150.1 57.333 75 28 2 159 231 287 359 9.68e-18 81.6
MsaG010910 AT4G36730.2 54.167 72 31 1 160 231 215 284 8.69e-16 75.5
MsaG010910 AT4G36730.1 54.167 72 31 1 160 231 217 286 9.57e-16 75.5

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTATCTTCAGGCATATTA+TGG 0.170895 2:+79292279 MsaT010910.1:intron
GTTGCCATCGCTTCCATTAC+TGG 0.238359 2:-79292930 None:intergenic
ATAAACAATACTAGTTATTC+TGG 0.247181 2:+79293094 MsaT010910.1:CDS
TGCATTGACTGCCGAGAATT+TGG 0.257432 2:+79294301 MsaT010910.1:CDS
GTTGGTGTCTCTATGCTTGT+AGG 0.275884 2:-79292669 None:intergenic
GTTGGACCTCTACTTTCTTC+AGG 0.305646 2:+79293498 MsaT010910.1:CDS
TTTGGAAGCGGCAGTCTCTT+TGG 0.337449 2:-79293435 None:intergenic
GCAACCAGTGCTGCAGAAAA+TGG 0.342628 2:+79293476 MsaT010910.1:CDS
CGAGAATTTGGTGCTGAAAT+CGG 0.374258 2:+79294313 MsaT010910.1:CDS
TGTGGAACACTGGTGGAAAT+AGG 0.380405 2:-79293555 None:intergenic
CGATTTCAGCACCAAATTCT+CGG 0.384484 2:-79294312 None:intergenic
ACAGGCTGAGGCTGAAGAAT+TGG 0.391234 2:+79294265 MsaT010910.1:intron
GCAGCAGCTGAGTTGAAATA+TGG 0.399898 2:-79292324 None:intergenic
ATCATCTGGAAACACTGATC+AGG 0.417420 2:+79292695 MsaT010910.1:CDS
GCCTCCTGAAGCTTGGATGC+AGG 0.426706 2:+79293593 MsaT010910.1:CDS
GAGACACCAACTAAATCATC+TGG 0.445527 2:+79292681 MsaT010910.1:CDS
TTCAACTCAGCTGCTGCTCC+TGG 0.457412 2:+79292330 MsaT010910.1:CDS
TGTATCTTCAGGCATATTAT+GGG 0.464465 2:+79292280 MsaT010910.1:intron
GAAATCGGAAATGAATGAAT+TGG 0.472002 2:+79294328 MsaT010910.1:CDS
TTCAGGTATGACCACATCAC+TGG 0.473232 2:+79293515 MsaT010910.1:CDS
AGTGGTTGTTGGTGTTCCAT+TGG 0.474613 2:-79293065 None:intergenic
GAAGATCCAGGCTTAGGCTT+AGG 0.474677 2:+79293808 MsaT010910.1:CDS
AAACTTACAGCAGGTGAAGA+TGG 0.476285 2:-79292538 None:intergenic
AGTACCTGCATCCAAGCTTC+AGG 0.494330 2:-79293597 None:intergenic
ACCAGTGTTCCACAGCCTTG+TGG 0.495543 2:+79293564 MsaT010910.1:CDS
GGAAGCGATGGCAACACTAC+AGG 0.500726 2:+79292938 MsaT010910.1:CDS
TTTATCATACCAGTGGTTGT+TGG 0.502643 2:-79293076 None:intergenic
GTGTTTCCAGATGATTTAGT+TGG 0.505094 2:-79292687 None:intergenic
CTGCTAGAAGATCCAGGCTT+AGG 0.520262 2:+79293802 MsaT010910.1:CDS
GGCAAAACTCCACAAGGCTG+TGG 0.541143 2:-79293573 None:intergenic
TTGCTTCCTAAGCCTAAGCC+TGG 0.544129 2:-79293814 None:intergenic
TCATCTGGAAACACTGATCA+GGG 0.547332 2:+79292696 MsaT010910.1:CDS
GTGAATCTGCTAGAAGATCC+AGG 0.554001 2:+79293796 MsaT010910.1:CDS
TCCACAAGGCTGTGGAACAC+TGG 0.554932 2:-79293565 None:intergenic
CCAGTGCTGCAGAAAATGGT+TGG 0.563429 2:+79293480 MsaT010910.1:CDS
GAAGCGATGGCAACACTACA+GGG 0.570248 2:+79292939 MsaT010910.1:CDS
AATGGAACACCAACAACCAC+TGG 0.585041 2:+79293067 MsaT010910.1:CDS
TTACTTGAACAGATGGAGAA+GGG 0.588445 2:+79293384 MsaT010910.1:intron
ATTACTTGAACAGATGGAGA+AGG 0.590419 2:+79293383 MsaT010910.1:intron
TGACCACATCACTGGAACTG+AGG 0.592621 2:+79293523 MsaT010910.1:CDS
ACAAGGCTGTGGAACACTGG+TGG 0.593279 2:-79293562 None:intergenic
ACTTCCAGTAATGGAAGCGA+TGG 0.593651 2:+79292926 MsaT010910.1:CDS
GTTCCTCAGTTCCAGTGATG+TGG 0.596954 2:-79293526 None:intergenic
AGTATTGTTTATCATACCAG+TGG 0.601348 2:-79293083 None:intergenic
GGGCTCCAGACTTCCAGTAA+TGG 0.604188 2:+79292917 MsaT010910.1:intron
ACACTGGTGGAAATAGGCGA+AGG 0.605436 2:-79293549 None:intergenic
CAATGTTATAAACAGACCAA+TGG 0.632904 2:+79293049 MsaT010910.1:intron
ACCTGCATCCAAGCTTCAGG+AGG 0.636392 2:-79293594 None:intergenic
GCTGAGTTGAAATATGGTGG+TGG 0.656136 2:-79292318 None:intergenic
TCAGGAGGCAAAACTCCACA+AGG 0.658647 2:-79293579 None:intergenic
CACTGGTGGAAATAGGCGAA+GGG 0.662789 2:-79293548 None:intergenic
AGAGTGAGCTGAAACGTGCG+AGG 0.666784 2:+79293757 MsaT010910.1:CDS
GTGAGCTGAAACGTGCGAGG+AGG 0.671296 2:+79293760 MsaT010910.1:CDS
GTCATACCTGAAGAAAGTAG+AGG 0.696910 2:-79293504 None:intergenic
GCAGCTGAGTTGAAATATGG+TGG 0.711269 2:-79292321 None:intergenic
AAGCGATGGCAACACTACAG+GGG 0.742066 2:+79292940 MsaT010910.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 79291593 79294409 79291593 ID=MsaG010910
Chr2 mRNA 79291593 79294409 79291593 ID=MsaT010910.1;Parent=MsaG010910
Chr2 exon 79291593 79291595 79291593 ID=MsaT010910.1.exon1;Parent=MsaT010910.1
Chr2 CDS 79291593 79291595 79291593 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Chr2 exon 79292291 79292351 79292291 ID=MsaT010910.1.exon2;Parent=MsaT010910.1
Chr2 CDS 79292291 79292351 79292291 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Chr2 exon 79292533 79292552 79292533 ID=MsaT010910.1.exon3;Parent=MsaT010910.1
Chr2 CDS 79292533 79292552 79292533 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Chr2 exon 79292660 79292737 79292660 ID=MsaT010910.1.exon4;Parent=MsaT010910.1
Chr2 CDS 79292660 79292737 79292660 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Chr2 exon 79292926 79292961 79292926 ID=MsaT010910.1.exon5;Parent=MsaT010910.1
Chr2 CDS 79292926 79292961 79292926 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Chr2 exon 79293064 79293129 79293064 ID=MsaT010910.1.exon6;Parent=MsaT010910.1
Chr2 CDS 79293064 79293129 79293064 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Chr2 exon 79293396 79293614 79293396 ID=MsaT010910.1.exon7;Parent=MsaT010910.1
Chr2 CDS 79293396 79293614 79293396 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Chr2 exon 79293753 79293836 79293753 ID=MsaT010910.1.exon8;Parent=MsaT010910.1
Chr2 CDS 79293753 79293836 79293753 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Chr2 exon 79294269 79294409 79294269 ID=MsaT010910.1.exon9;Parent=MsaT010910.1
Chr2 CDS 79294269 79294409 79294269 ID=cds.MsaT010910.1;Parent=MsaT010910.1
Gene Sequence

>MsaT010910.1

ATGGCATATTATGGGCAAAGAGTCAACATTCCACCACCATATTTCAACTCAGCTGCTGCTCCTGGAATTCCATCTTCACCTGCTGCTGGAACTCCTACAAGCATAGAGACACCAACTAAATCATCTGGAAACACTGATCAGGGTTTAATCAAAAAATTGAAAACTTCCAGTAATGGAAGCGATGGCAACACTACAGGGACCAATGGAACACCAACAACCACTGGTATGATAAACAATACTAGTTATTCTGGATTACAAATATACATGGAGAAGGGAAAACTGAGATGCAAGATAATCAAGTTTCCAAAGAGACTGCCGCTTCCAAAAAGACGGTGTCAGTTATCGCAACCAGTGCTGCAGAAAATGGTTGGACCTCTACTTTCTTCAGGTATGACCACATCACTGGAACTGAGGAACCCTTCGCCTATTTCCACCAGTGTTCCACAGCCTTGTGGAGTTTTGCCTCCTGAAGCTTGGATGCAGAATGAGAGTGAGCTGAAACGTGCGAGGAGGAAACAATCAAATCGTGAATCTGCTAGAAGATCCAGGCTTAGGCTTAGGAAGCAAGCTGAGGCTGAAGAATTGGCACGAAGAGTCGATGCATTGACTGCCGAGAATTTGGTGCTGAAATCGGAAATGAATGAATTGGCTGAAAATTCTGAGAAGCTGAAGATTGAAAATGCTACATTAAAGATAATGCACACATAA
Protein sequence

>MsaT010910.1

MAYYGQRVNIPPPYFNSAAAPGIPSSPAAGTPTSIETPTKSSGNTDQGLIKKLKTSSNGSDGNTTGTNGTPTTTGMINNTSYSGLQIYMEKGKLRCKIIKFPKRLPLPKRRCQLSQPVLQKMVGPLLSSGMTTSLELRNPSPISTSVPQPCGVLPPEAWMQNESELKRARRKQSNRESARRSRLRLRKQAEAEELARRVDALTAENLVLKSEMNELAENSEKLKIENATLKIMHT*