AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010931


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010931 MtrunA17_Chr2g0330641 92.982 399 21 1 1 399 1 392 0.0 718
MsaG010931 MtrunA17_Chr8g0341421 51.087 460 111 22 1 388 1 418 5.15e-105 317
MsaG010931 MtrunA17_Chr3g0134001 52.000 125 55 3 268 392 143 262 8.81e-35 129
MsaG010931 MtrunA17_Chr8g0384141 63.043 92 33 1 269 360 87 177 4.39e-34 127
MsaG010931 MtrunA17_Chr5g0402161 61.682 107 39 2 258 363 98 203 1.67e-28 112
MsaG010931 MtrunA17_Chr8g0375121 55.970 134 42 4 243 363 69 198 2.83e-28 111
MsaG010931 MtrunA17_Chr7g0243041 52.941 68 32 0 303 370 32 99 7.72e-20 85.5
MsaG010931 MtrunA17_Chr1g0152651 62.963 54 20 0 306 359 283 336 7.37e-17 81.3
MsaG010931 MtrunA17_Chr5g0435021 58.929 56 23 0 304 359 225 280 3.86e-16 78.6
MsaG010931 MtrunA17_Chr1g0207301 49.254 67 34 0 293 359 284 350 9.84e-16 78.2
MsaG010931 MtrunA17_Chr5g0393721 55.357 56 25 0 304 359 231 286 2.07e-15 76.6
MsaG010931 MtrunA17_Chr1g0207311 55.357 56 25 0 304 359 203 258 3.41e-15 75.5
MsaG010931 MtrunA17_Chr3g0134741 50.794 63 31 0 297 359 229 291 8.88e-15 74.7
MsaG010931 MtrunA17_Chr1g0179061 51.562 64 31 0 297 360 170 233 1.11e-14 73.6
MsaG010931 MtrunA17_Chr2g0311101 58.491 53 22 0 308 360 180 232 1.22e-13 71.2
MsaG010931 MtrunA17_Chr4g0074161 55.357 56 25 0 304 359 213 268 1.94e-13 71.2
MsaG010931 MtrunA17_Chr1g0190731 55.357 56 25 0 304 359 200 255 5.66e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010931 AT3G21330.1 54.639 291 118 8 105 391 86 366 3.88e-83 259
MsaG010931 AT4G00120.1 61.798 89 33 1 272 360 88 175 1.97e-29 113
MsaG010931 AT3G50330.1 52.941 136 60 4 227 360 49 182 6.30e-26 105
MsaG010931 AT5G67060.2 64.130 92 32 1 269 360 83 173 9.40e-26 104
MsaG010931 AT5G67060.1 64.130 92 32 1 269 360 95 185 1.04e-25 104
MsaG010931 AT5G09750.1 62.366 93 34 1 269 361 92 183 4.67e-24 99.8
MsaG010931 AT5G01305.1 57.353 68 29 0 295 362 35 102 1.94e-20 88.2
MsaG010931 AT1G66470.1 39.450 109 64 1 251 359 151 257 6.02e-17 81.3
MsaG010931 AT5G37800.1 46.067 89 40 1 279 359 175 263 1.14e-16 80.5
MsaG010931 AT5G43175.1 46.269 67 36 0 293 359 128 194 3.69e-16 77.4
MsaG010931 AT4G33880.1 53.226 62 29 0 298 359 267 328 5.60e-16 79.0
MsaG010931 AT2G14760.1 53.226 62 29 0 298 359 238 299 8.55e-16 78.2
MsaG010931 AT1G27740.1 50.000 62 31 0 298 359 168 229 8.19e-15 74.3
MsaG010931 AT4G30980.1 54.545 66 29 1 295 359 127 192 1.20e-14 74.7
MsaG010931 AT4G30980.2 54.545 66 29 1 295 359 175 240 1.79e-14 74.7
MsaG010931 AT2G24260.2 57.143 56 24 0 304 359 42 97 1.88e-14 73.2
MsaG010931 AT2G24260.1 57.143 56 24 0 304 359 145 200 3.40e-14 73.6
MsaG010931 AT5G58010.1 49.275 69 34 1 292 359 93 161 7.35e-13 69.3
MsaG010931 AT2G14760.3 44.000 75 29 1 298 359 238 312 1.24e-12 68.9

Find 84 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTTCTTCTACTGAATT+TGG 0.150846 2:-79493391 MsaT010931.1:CDS
GAATATTGGATGTTGAAATT+AGG 0.176056 2:+79492857 None:intergenic
CATCTTGGTTTCTTTGACTT+TGG 0.197671 2:+79493343 None:intergenic
ATTTAGGCCGGTGACGAATT+TGG 0.200299 2:-79493182 MsaT010931.1:CDS
GATTTCAATCATGGGAATAT+TGG 0.228711 2:+79492843 None:intergenic
GACAATGAAACTGATGAATT+TGG 0.244336 2:+79493277 None:intergenic
AATATTGGATGTTGAAATTA+GGG 0.257470 2:+79492858 None:intergenic
AACTTTAGTGCAGTTTCTTC+TGG 0.287864 2:-79493598 MsaT010931.1:CDS
CCAAGGTTCATTCCACAAAA+AGG 0.291280 2:-79493462 MsaT010931.1:CDS
TCCCAACTGTTTCCAGCTCC+TGG 0.305187 2:+79493868 None:intergenic
TTCATTTGTGCTATTGCCTC+TGG 0.321358 2:+79493229 None:intergenic
TTTAGGCCGGTGACGAATTT+GGG 0.332170 2:-79493181 MsaT010931.1:CDS
AAGTGATGGAAAAGCCTAAA+AGG 0.336703 2:-79493153 MsaT010931.1:CDS
AAGGAGAAGGCTATGTTTGC+AGG 0.348753 2:+79492905 None:intergenic
TGCCTAGCCGCCACAGTTTG+TGG 0.350175 2:+79493106 None:intergenic
CTTGGTTTCTTTGACTTTGG+TGG 0.356806 2:+79493346 None:intergenic
GATGATAATGATTTCAATCA+TGG 0.362071 2:+79492834 None:intergenic
TGTGCTATTGCCTCTGGATC+AGG 0.366599 2:+79493235 None:intergenic
TATATAGAGCTGCAGCATTT+AGG 0.373879 2:-79493198 MsaT010931.1:CDS
CCAATCATACCCTTCACTTT+CGG 0.383334 2:-79493830 MsaT010931.1:CDS
TACATATGGATTGTCTTAGC+TGG 0.402802 2:-79494029 None:intergenic
AAACTGATGAATTTGGTTGC+TGG 0.410865 2:+79493284 None:intergenic
GCCGGTGACGAATTTGGGTT+TGG 0.412280 2:-79493176 MsaT010931.1:CDS
GTCTTGCAGAAGATTGTTCC+TGG 0.420469 2:-79493052 MsaT010931.1:CDS
TGCATTCAAGGAACTGGATC+AGG 0.432172 2:-79493533 MsaT010931.1:CDS
CCGAAAGTGAAGGGTATGAT+TGG 0.436711 2:+79493830 None:intergenic
GCAGTATGCATTCAAGGAAC+TGG 0.437343 2:-79493539 MsaT010931.1:CDS
CATTGATGCAGTGTCCATCT+TGG 0.445318 2:+79493024 None:intergenic
TCATGGAAATTCCATCATCT+TGG 0.445903 2:+79493653 None:intergenic
TACTCTTGCTGATTGGTTGG+TGG 0.446984 2:+79493755 None:intergenic
CTGTGGCGGCTAGGCAAAGA+AGG 0.454660 2:-79493099 MsaT010931.1:CDS
GGACACTTATTATCCCATCT+TGG 0.463511 2:+79493328 None:intergenic
TAGAGCACAAGTCAAAGAAC+TGG 0.468462 2:-79492969 MsaT010931.1:CDS
ACTAATTCTGTCCAAGATGA+TGG 0.470459 2:-79493664 MsaT010931.1:CDS
TTACCTTGATCTGAGGAAGC+TGG 0.471387 2:+79493499 None:intergenic
GTAGTAGTACTCTTGCTGAT+TGG 0.471486 2:+79493748 None:intergenic
GTCAAAGAACTGGAGAATCT+AGG 0.474273 2:-79492959 MsaT010931.1:CDS
GGTTGAGTGTTCCACAAGAT+TGG 0.479415 2:+79493976 None:intergenic
GGACATGCAGTATGCATTCA+AGG 0.489351 2:-79493545 MsaT010931.1:CDS
CATGGGAATTGACTGGTTGA+AGG 0.490980 2:+79492886 None:intergenic
TGGTGGGCCATGTTGAAATC+AGG 0.494073 2:+79493772 None:intergenic
CAGAAGGTTCAACCAGGAGC+TGG 0.494279 2:-79493880 MsaT010931.1:CDS
ACTCTTGCTGATTGGTTGGT+GGG 0.498014 2:+79493756 None:intergenic
GTTCCACAAGATTGGTATGT+TGG 0.503170 2:+79493984 None:intergenic
ACCAGGAGCTGGAAACAGTT+GGG 0.515648 2:-79493869 MsaT010931.1:CDS
TAGTACTCTTGCTGATTGGT+TGG 0.516116 2:+79493752 None:intergenic
ACATATGGATTGTCTTAGCT+GGG 0.522229 2:-79494028 None:intergenic
GAATTTGGGTTTGGAAGTGA+TGG 0.527963 2:-79493167 MsaT010931.1:CDS
TCTCCAGCTTCCTCAGATCA+AGG 0.534533 2:-79493502 MsaT010931.1:CDS
TTACCAACATACCAATCTTG+TGG 0.535200 2:-79493987 MsaT010931.1:CDS
AACCAGGAGCTGGAAACAGT+TGG 0.539885 2:-79493870 MsaT010931.1:CDS
AGAGGCAATAGCACAAATGA+AGG 0.541238 2:-79493227 MsaT010931.1:CDS
CTCTGGATCAGGCTCTTCAA+AGG 0.548876 2:+79493246 None:intergenic
ATGATAATGATTTCAATCAT+GGG 0.556089 2:+79492835 None:intergenic
AGATGGGATAATAAGTGTCC+TGG 0.557228 2:-79493325 MsaT010931.1:CDS
GTGTCCATCTTGGTTCCACC+AGG 0.564056 2:+79493034 None:intergenic
TAAAATCATTGATAATTCCA+AGG 0.564539 2:-79493479 MsaT010931.1:CDS
TAGTCTCTTGCAAAGTCTTG+TGG 0.568681 2:+79493955 None:intergenic
TGTTCCTGGTGGAACCAAGA+TGG 0.571863 2:-79493038 MsaT010931.1:CDS
TCGATATTGACCGAAAGTGA+AGG 0.573714 2:+79493820 None:intergenic
GAAGGGAAAGAATAAGTGAG+AGG 0.579170 2:-79493081 MsaT010931.1:CDS
TCCAAACCCAAATTCGTCAC+CGG 0.582453 2:+79493175 None:intergenic
ATCCACAAACTGTGGCGGCT+AGG 0.587768 2:-79493108 MsaT010931.1:CDS
AATTGACTGGTTGAAGGAGA+AGG 0.590555 2:+79492892 None:intergenic
CAAAGTCAAAGAAACCAAGA+TGG 0.591227 2:-79493342 MsaT010931.1:CDS
CGATATTGACCGAAAGTGAA+GGG 0.597835 2:+79493821 None:intergenic
TAGAGCTGCAGCATTTAGGC+CGG 0.600006 2:-79493194 MsaT010931.1:CDS
AAAGTCAAAGAAACCAAGAT+GGG 0.607623 2:-79493341 MsaT010931.1:CDS
CTTTGAAGAGCCTGATCCAG+AGG 0.622335 2:-79493245 MsaT010931.1:CDS
GCCATGTTGAAATCAGGCAG+TGG 0.628594 2:+79493778 None:intergenic
TGAGTAGTAATGCTGAAGCA+AGG 0.639766 2:+79493418 None:intergenic
TCAAATGCAAGAAATGCAGA+AGG 0.642036 2:-79493896 MsaT010931.1:CDS
AAATTGATATTTGATGAACC+AGG 0.643576 2:+79493307 None:intergenic
AGTGGCACATAATTGTTATG+TGG 0.644546 2:+79493796 None:intergenic
TTGCAGAAGATTGTTCCTGG+TGG 0.647074 2:-79493049 MsaT010931.1:CDS
ATCAAATGATCCACAAACTG+TGG 0.655439 2:-79493116 MsaT010931.1:CDS
GAAATGCAGAAGGTTCAACC+AGG 0.659735 2:-79493886 MsaT010931.1:CDS
GTCAGTGTTACTGATAGTAG+TGG 0.661499 2:+79493687 None:intergenic
GCGTACAAATGCTAGAAACA+AGG 0.665015 2:-79493566 MsaT010931.1:CDS
AAATGATCCACAAACTGTGG+CGG 0.666151 2:-79493113 MsaT010931.1:CDS
TGTGGCGGCTAGGCAAAGAA+GGG 0.669512 2:-79493098 MsaT010931.1:CDS
GAGAAGGCTATGTTTGCAGG+AGG 0.669932 2:+79492908 None:intergenic
GCCACTGCCTGATTTCAACA+TGG 0.684690 2:-79493779 MsaT010931.1:CDS
CTTGTGCTCTAAGAAACTTG+AGG 0.702293 2:+79492981 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 79492847 79494046 79492847 ID=MsaG010931
Chr2 mRNA 79492847 79494046 79492847 ID=MsaT010931.1;Parent=MsaG010931
Chr2 exon 79492847 79494046 79492847 ID=MsaT010931.1.exon1;Parent=MsaT010931.1
Chr2 CDS 79492847 79494046 79492847 ID=cds.MsaT010931.1;Parent=MsaT010931.1
Gene Sequence

>MsaT010931.1

ATGGATTGTCTTAGCTGGGATGATGATACACAACTTGTTACCAACATACCAATCTTGTGGAACACTCAACCACAAGACTTTGCAAGAGACTATTCAATGTCCAACCCAAGTTCAAGTGTTTTTAACTATCAAATGCAAGAAATGCAGAAGGTTCAACCAGGAGCTGGAAACAGTTGGGAAGAAACTTTAATATTCCAATCATACCCTTCACTTTCGGTCAATATCGATCCACATAACAATTATGTGCCACTGCCTGATTTCAACATGGCCCACCAACCAATCAGCAAGAGTACTACTACATGCTCACTTGAATCATCACTTGATTGTTTGCTTTCAGCCACTACTATCAGTAACACTGACACTAATTCTGTCCAAGATGATGGAATTTCCATGATTTTTTCTGACTGCAAAAACAGAAACTTATGGAACTTTAGTGCAGTTTCTTCTGGTGAATCTGAGCGTACAAATGCTAGAAACAAGGACATGCAGTATGCATTCAAGGAACTGGATCAGGAAACAGTATCTCCAGCTTCCTCAGATCAAGGTAAAATCATTGATAATTCCAAGGTTCATTCCACAAAAAGGACTAATATTGATCAATATGATCCTTGCTTCAGCATTACTACTCAGAATTCTTCTTCTTCTACTGAATTTGGTTTCAAGCTTATCTCAGAGAAACCACCAAAGTCAAAGAAACCAAGATGGGATAATAAGTGTCCTGGTTCATCAAATATCAATTTCCAGCAACCAAATTCATCAGTTTCATTGTCAAATTCATCCTTTGAAGAGCCTGATCCAGAGGCAATAGCACAAATGAAGGAAATGATATATAGAGCTGCAGCATTTAGGCCGGTGACGAATTTGGGTTTGGAAGTGATGGAAAAGCCTAAAAGGAAGAATGTTAAGATATCAAATGATCCACAAACTGTGGCGGCTAGGCAAAGAAGGGAAAGAATAAGTGAGAGGATTAGAGTCTTGCAGAAGATTGTTCCTGGTGGAACCAAGATGGACACTGCATCAATGCTTGATGAAGCTGCTAATTACCTCAAGTTTCTTAGAGCACAAGTCAAAGAACTGGAGAATCTAGGAAACAAGATTGATACAATGAATAATTGTCCTCCTGCAAACATAGCCTTCTCCTTCAACCAGTCAATTCCCATGCAAAACCCTAATTTCAACATCCAATATTCCCATGATTGA

Protein sequence

>MsaT010931.1

MDCLSWDDDTQLVTNIPILWNTQPQDFARDYSMSNPSSSVFNYQMQEMQKVQPGAGNSWEETLIFQSYPSLSVNIDPHNNYVPLPDFNMAHQPISKSTTTCSLESSLDCLLSATTISNTDTNSVQDDGISMIFSDCKNRNLWNFSAVSSGESERTNARNKDMQYAFKELDQETVSPASSDQGKIIDNSKVHSTKRTNIDQYDPCFSITTQNSSSSTEFGFKLISEKPPKSKKPRWDNKCPGSSNINFQQPNSSVSLSNSSFEEPDPEAIAQMKEMIYRAAAFRPVTNLGLEVMEKPKRKNVKISNDPQTVAARQRRERISERIRVLQKIVPGGTKMDTASMLDEAANYLKFLRAQVKELENLGNKIDTMNNCPPANIAFSFNQSIPMQNPNFNIQYSHD*