AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010947


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010947 MtrunA17_Chr2g0330821 91.477 352 28 1 1 352 1 350 0.0 660
MsaG010947 MtrunA17_Chr8g0378061 66.906 278 72 3 80 340 23 297 2.25e-122 361
MsaG010947 MtrunA17_Chr6g0467041 54.985 331 109 6 60 353 1 328 3.72e-112 330
MsaG010947 MtrunA17_Chr2g0330841 67.511 237 57 3 123 341 24 258 6.06e-97 293
MsaG010947 MtrunA17_Chr5g0410881 48.990 198 90 1 67 253 20 217 2.32e-57 194
MsaG010947 MtrunA17_Chr8g0379701 47.716 197 91 2 60 252 1 189 2.62e-51 176
MsaG010947 MtrunA17_Chr4g0039891 45.946 185 90 1 79 253 12 196 1.74e-47 167
MsaG010947 MtrunA17_Chr1g0204741 43.684 190 98 2 73 256 34 220 4.68e-47 162
MsaG010947 MtrunA17_Chr1g0185131 41.799 189 103 1 65 253 18 199 4.68e-44 155
MsaG010947 MtrunA17_Chr4g0071051 42.632 190 104 3 73 261 95 280 3.35e-43 155
MsaG010947 MtrunA17_Chr7g0260331 39.545 220 126 1 44 256 14 233 3.39e-43 153
MsaG010947 MtrunA17_Chr3g0135831 40.625 192 110 1 74 261 41 232 1.59e-42 152
MsaG010947 MtrunA17_Chr8g0392001 45.455 187 91 4 78 253 21 207 7.82e-39 142
MsaG010947 MtrunA17_Chr6g0485571 38.191 199 95 2 78 262 10 194 2.99e-36 134
MsaG010947 MtrunA17_Chr2g0284481 37.143 210 111 5 60 258 2 201 2.69e-35 132
MsaG010947 MtrunA17_Chr5g0402291 56.731 104 45 0 76 179 6 109 2.14e-34 128
MsaG010947 MtrunA17_Chr5g0397881 54.808 104 47 0 67 170 12 115 3.97e-34 129
MsaG010947 MtrunA17_Chr3g0134031 63.830 94 34 0 77 170 22 115 5.13e-34 129
MsaG010947 MtrunA17_Chr5g0441531 55.882 102 45 0 69 170 15 116 5.60e-32 120
MsaG010947 MtrunA17_Chr1g0203601 54.167 96 44 0 77 172 16 111 1.61e-31 120
MsaG010947 MtrunA17_Chr6g0472421 53.191 94 44 0 77 170 31 124 4.56e-30 117
MsaG010947 MtrunA17_Chr7g0257331 51.064 94 46 0 77 170 20 113 5.03e-29 112
MsaG010947 MtrunA17_Chr8g0375041 52.688 93 44 0 78 170 8 100 1.64e-27 108
MsaG010947 MtrunA17_Chr4g0047421 51.020 98 44 1 77 170 31 128 4.76e-27 106
MsaG010947 MtrunA17_Chr1g0179321 61.667 60 23 0 79 138 49 108 7.52e-22 89.4
MsaG010947 MtrunA17_Chr4g0023771 60.656 61 23 1 79 138 15 75 6.82e-19 81.3
MsaG010947 MtrunA17_Chr2g0330871 84.444 45 7 0 1 45 313 357 1.23e-16 80.9
MsaG010947 MtrunA17_Chr6g0470901 51.648 91 35 3 253 340 38 122 6.95e-16 74.3
MsaG010947 MtrunA17_Chr1g0179311 32.283 127 84 1 140 264 15 141 9.92e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010947 AT4G13980.1 50.515 291 122 3 80 352 24 310 3.80e-85 266
MsaG010947 AT1G32330.2 49.057 212 89 3 55 253 19 224 4.30e-53 182
MsaG010947 AT1G32330.1 49.057 212 89 3 55 253 19 224 5.87e-53 182
MsaG010947 AT4G17750.1 45.894 207 94 1 65 253 38 244 1.11e-51 179
MsaG010947 AT5G16820.1 47.059 187 90 1 79 256 27 213 2.55e-49 172
MsaG010947 AT5G16820.2 47.059 187 90 1 79 256 27 213 2.55e-49 172
MsaG010947 AT3G02990.1 48.387 186 86 2 79 255 23 207 1.62e-48 170
MsaG010947 AT2G26150.1 41.485 229 122 4 73 290 38 265 3.27e-47 164
MsaG010947 AT2G26150.4 41.485 229 122 4 73 290 38 265 3.27e-47 164
MsaG010947 AT3G22830.1 41.237 194 103 1 74 256 56 249 1.18e-45 161
MsaG010947 AT5G43840.1 43.085 188 96 2 77 263 17 194 6.96e-45 156
MsaG010947 AT5G03720.2 43.316 187 103 2 74 257 49 235 7.05e-44 157
MsaG010947 AT5G03720.1 43.316 187 103 2 74 257 49 235 7.05e-44 157
MsaG010947 AT3G51910.1 41.848 184 96 1 73 256 23 195 1.35e-43 152
MsaG010947 AT4G18880.1 49.721 179 85 1 79 252 15 193 6.85e-42 151
MsaG010947 AT3G63350.1 38.500 200 123 0 57 256 6 205 1.27e-41 147
MsaG010947 AT5G45710.3 44.681 188 97 2 72 252 3 190 2.60e-41 148
MsaG010947 AT5G45710.1 44.681 188 97 2 72 252 3 190 2.60e-41 148
MsaG010947 AT1G67970.1 42.541 181 99 2 78 253 18 198 6.41e-39 142
MsaG010947 AT3G24520.1 42.045 176 83 3 78 253 16 172 3.03e-38 140
MsaG010947 AT1G46264.1 53.782 119 45 1 71 179 26 144 1.13e-34 130
MsaG010947 AT5G54070.1 37.363 182 109 1 75 256 67 243 8.48e-34 128
MsaG010947 AT5G62020.1 49.495 99 50 0 77 175 21 119 4.06e-30 117
MsaG010947 AT4G36990.1 50.943 106 52 0 67 172 2 107 6.49e-30 116
MsaG010947 AT2G41690.1 46.154 117 63 0 77 193 38 154 2.60e-29 114
MsaG010947 AT4G11660.1 51.064 94 46 0 77 170 57 150 1.56e-28 114
MsaG010947 AT4G18870.1 43.434 99 52 2 71 166 2 99 8.09e-18 83.2
MsaG010947 AT4G18870.1 40.909 88 52 0 79 166 146 233 3.26e-17 81.6
MsaG010947 AT1G77570.1 37.000 100 60 2 70 166 17 116 2.72e-15 72.8
MsaG010947 AT2G26150.3 33.333 162 96 5 140 290 50 210 9.37e-14 71.2
MsaG010947 AT2G26150.2 33.333 162 96 5 140 290 50 210 9.37e-14 71.2

Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTAAAGCTTCCCTTTC+TGG 0.004045 2:+79595527 None:intergenic
AAGTTTCAACTGTCATTATT+TGG 0.186856 2:+79595005 None:intergenic
GAAATTGTTGTGTTTGAAAT+AGG 0.280393 2:+79596655 None:intergenic
TCCTACAGTGTATATTCTTC+AGG 0.294189 2:+79595594 None:intergenic
GTTTATTCGAAAATTCTTGA+TGG 0.334012 2:+79595222 None:intergenic
CTACTTTACTTGATCATGTT+AGG 0.345999 2:-79595331 MsaT010947.1:CDS
CCAACAACAGCTTTGTGGTT+TGG 0.357531 2:-79596712 MsaT010947.1:CDS
GGAGAACGTAAGCGAATTCC+AGG 0.364208 2:+79596684 None:intergenic
TCAGATATGAACTTTGTATC+AGG 0.375340 2:-79595173 MsaT010947.1:CDS
AATAGCTGAAGGTGCAGGTC+AGG 0.377621 2:-79597713 MsaT010947.1:CDS
ATTTGGATTTGCTTGATAGT+AGG 0.385790 2:+79595022 None:intergenic
TCCAGAAAGGGAAGCTTTAG+AGG 0.389076 2:-79595528 MsaT010947.1:CDS
TCGATAGATGTGTCATTAGA+AGG 0.393975 2:+79597682 None:intergenic
CGAAGGCCGTCTCTTGTCTC+TGG 0.412614 2:+79596868 None:intergenic
CTTCAGACAAATGTTTCTGC+AGG 0.414385 2:+79595111 None:intergenic
CAATCATTACAATCTCAGTA+TGG 0.432196 2:-79595261 MsaT010947.1:CDS
CTCTACCAACAACAGCTTTG+TGG 0.452629 2:-79596717 MsaT010947.1:CDS
CTCGAGTCCAGGTTATTAAA+CGG 0.459756 2:-79595470 MsaT010947.1:CDS
CGCTGAAAATGAAGGAGGTC+CGG 0.461435 2:-79596807 MsaT010947.1:CDS
AACGACTTGTTTCAAGATGT+TGG 0.464169 2:-79597637 MsaT010947.1:CDS
CCACAGTCATAGTCATCCTC+CGG 0.469987 2:-79595564 MsaT010947.1:CDS
AAGTCGTTCGATGAAGCATC+TGG 0.472231 2:+79597652 None:intergenic
CCAAACCACAAAGCTGTTGT+TGG 0.484501 2:+79596712 None:intergenic
GAGACGGCCTTCGAGTCAAA+TGG 0.486664 2:-79596858 MsaT010947.1:CDS
TTTGTCAAACTTCTTTGAGA+AGG 0.488131 2:-79595366 MsaT010947.1:CDS
TGAATCAACAAAGTTTCAGC+TGG 0.492809 2:-79595435 MsaT010947.1:CDS
TCATACATCTTCTGAACAAA+CGG 0.493257 2:+79596782 None:intergenic
AACCGTGCCGTTTAATAACC+TGG 0.495001 2:+79595463 None:intergenic
CGTCTCTTGTCTCTGGATGT+TGG 0.495712 2:+79596875 None:intergenic
GTGGTTATCTGAGAAAATTA+AGG 0.508725 2:-79597611 MsaT010947.1:intron
CTAACATGATCAAGTAAAGT+AGG 0.518656 2:+79595332 None:intergenic
TTGTTGTGTTTGAAATAGGT+TGG 0.526405 2:+79596659 None:intergenic
CGAGCAGCTACTGGATGGCA+TGG 0.526948 2:-79595405 MsaT010947.1:CDS
ATTATATGCCGCCGCATCCA+TGG 0.527906 2:+79595295 None:intergenic
ATACATAGGTATCGAGCTGA+TGG 0.540406 2:+79596621 None:intergenic
TCAGCGGCGGTGGATGAGGA+TGG 0.546046 2:+79596824 None:intergenic
TCCACCGCCGCTGAAAATGA+AGG 0.550734 2:-79596815 MsaT010947.1:CDS
CCGGAGGATGACTATGACTG+TGG 0.551542 2:+79595564 None:intergenic
ATCGAGCTGATGGATGAAGC+TGG 0.552111 2:+79596631 None:intergenic
CACAGTCATAGTCATCCTCC+GGG 0.559092 2:-79595563 MsaT010947.1:CDS
TTCTGCTGTAGATCCAGAAA+GGG 0.561267 2:-79595540 MsaT010947.1:CDS
ATCTTCTGAACAAACGGCGC+CGG 0.569912 2:+79596788 None:intergenic
ATAGTAATAGCTGAAGGTGC+AGG 0.572259 2:-79597718 MsaT010947.1:CDS
CAATATCGAATCCATGGATG+CGG 0.572324 2:-79595306 MsaT010947.1:CDS
TACTGGATGGCATGGAGAAG+AGG 0.574862 2:-79595397 MsaT010947.1:CDS
TAGGCGCAATATCGAATCCA+TGG 0.575915 2:-79595312 MsaT010947.1:CDS
GTTCTGCTGTAGATCCAGAA+AGG 0.579439 2:-79595541 MsaT010947.1:CDS
CTGCAGAAACATTTGTCTGA+AGG 0.579693 2:-79595110 MsaT010947.1:CDS
TGTGTTTGAAATAGGTTGGA+AGG 0.588259 2:+79596663 None:intergenic
TGTTTCAAGATGTTGGTCTG+TGG 0.592209 2:-79597630 MsaT010947.1:CDS
CGACGGACGATATCGTCTCA+TGG 0.592310 2:-79596742 MsaT010947.1:CDS
ACATGTTTCAAATGAAGATG+AGG 0.605669 2:-79595138 MsaT010947.1:CDS
GCAGCATCCATTTGACTCGA+AGG 0.608828 2:+79596851 None:intergenic
GTCCAGGTTATTAAACGGCA+CGG 0.609349 2:-79595465 MsaT010947.1:CDS
TCTGGATCTACAGCAGAACC+CGG 0.614712 2:+79595545 None:intergenic
GGATGACTATGACTGTGGAT+AGG 0.617132 2:+79595569 None:intergenic
TCCTGAAGAATATACACTGT+AGG 0.623219 2:-79595595 MsaT010947.1:CDS
ATGGTTATAGTAATAGCTGA+AGG 0.630661 2:-79597724 MsaT010947.1:CDS
CAGACAAGAACTGAAAGACC+AGG 0.634076 2:-79595071 MsaT010947.1:CDS
TATCGAATCCATGGATGCGG+CGG 0.638808 2:-79595303 MsaT010947.1:CDS
GAGAACGTAAGCGAATTCCA+GGG 0.643353 2:+79596685 None:intergenic
AAGTTCATATCTGAGACAGA+TGG 0.645901 2:+79595182 None:intergenic
AGGCTGTTCAGAAAATGAAG+AGG 0.658976 2:-79595051 MsaT010947.1:CDS
AGAACGTAAGCGAATTCCAG+GGG 0.674620 2:+79596686 None:intergenic
CAACATCCAGAGACAAGAGA+CGG 0.680432 2:-79596874 MsaT010947.1:CDS
TCAGAAGATGTATGACATGG+TGG 0.705908 2:-79596774 MsaT010947.1:CDS
CATGGTGGATGATTCCGCGA+CGG 0.706453 2:-79596759 MsaT010947.1:CDS
ACCGCCGCTGAAAATGAAGG+AGG 0.719377 2:-79596812 MsaT010947.1:CDS
TGTTCAGAAGATGTATGACA+TGG 0.734293 2:-79596777 MsaT010947.1:CDS
GGATCTACAGCAGAACCCGG+AGG 0.744225 2:+79595548 None:intergenic
TGAGACGATATCGTCCGTCG+CGG 0.744820 2:+79596745 None:intergenic
GAACGTAAGCGAATTCCAGG+GGG 0.753677 2:+79596687 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 79595010 79597746 79595010 ID=MsaG010947
Chr2 mRNA 79595010 79597746 79595010 ID=MsaT010947.1;Parent=MsaG010947
Chr2 exon 79595010 79595687 79595010 ID=MsaT010947.1.exon3;Parent=MsaT010947.1
Chr2 CDS 79595010 79595687 79595010 ID=cds.MsaT010947.1;Parent=MsaT010947.1
Chr2 exon 79596625 79596903 79596625 ID=MsaT010947.1.exon2;Parent=MsaT010947.1
Chr2 CDS 79596625 79596903 79596625 ID=cds.MsaT010947.1;Parent=MsaT010947.1
Chr2 exon 79597612 79597746 79597612 ID=MsaT010947.1.exon1;Parent=MsaT010947.1
Chr2 CDS 79597612 79597746 79597612 ID=cds.MsaT010947.1;Parent=MsaT010947.1
Gene Sequence

>MsaT010947.1

ATGGTTATAGTAATAGCTGAAGGTGCAGGTCAGGAGCTAATTCCTTCTAATGACACATCTATCGAGAATAAGCCAGATGCTTCATCGAACGACTTGTTTCAAGATGTTGGTCTGTGGTTATCTGAGAAAATTAAGCAAACGCCAACATCCAGAGACAAGAGACGGCCTTCGAGTCAAATGGATGCTGCACAGCCATCCTCATCCACCGCCGCTGAAAATGAAGGAGGTCCGGCGCCGTTTGTTCAGAAGATGTATGACATGGTGGATGATTCCGCGACGGACGATATCGTCTCATGGAGCTCTACCAACAACAGCTTTGTGGTTTGGAACCCCCTGGAATTCGCTTACGTTCTCCTTCCAACCTATTTCAAACACAACAATTTCTCCAGCTTCATCCATCAGCTCGATACCTATGAATTTAGAAAAATTGATTCGGAGCGTTGCGAGTTTGCTAATGAAGAATTTATAGAAGATCAAAAACATCTCCTGAAGAATATACACTGTAGGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTGCTGTAGATCCAGAAAGGGAAGCTTTAGAGGAAGAAATAGAGAAGCTTTCCCAAGAGAAAAATTCGCTCGAGTCCAGGTTATTAAACGGCACGGTTGATGTTGAATCAACAAAGTTTCAGCTGGATGTTTTCGAGCAGCTACTGGATGGCATGGAGAAGAGGCAGACAAGTTTGTCAAACTTCTTTGAGAAGGCTCTTCAGAATCCTACTTTACTTGATCATGTTAGGCGCAATATCGAATCCATGGATGCGGCGGCATATAATAGCTTGATTGTCAATCATTACAATCTCAGTATGGAATTTGAAAATGCTTTCCATCAAGAATTTTCGAATAAACTTAGACTGGAATTGTCACCATCTGTCTCAGATATGAACTTTGTATCAGGTAGCAGTAGCACACATGTTTCAAATGAAGATGAGGAAAGCCTGCAGAAACATTTGTCTGAAGGAGAACTGACAGAAATGCAGACAAGAACTGAAAGACCAGGCTGTTCAGAAAATGAAGAGGCAATATCCTACTATCAAGCAAATCCAAATAATGACAGTTGA

Protein sequence

>MsaT010947.1

MVIVIAEGAGQELIPSNDTSIENKPDASSNDLFQDVGLWLSEKIKQTPTSRDKRRPSSQMDAAQPSSSTAAENEGGPAPFVQKMYDMVDDSATDDIVSWSSTNNSFVVWNPLEFAYVLLPTYFKHNNFSSFIHQLDTYEFRKIDSERCEFANEEFIEDQKHLLKNIHCRKPIHSHSHPPGSAVDPEREALEEEIEKLSQEKNSLESRLLNGTVDVESTKFQLDVFEQLLDGMEKRQTSLSNFFEKALQNPTLLDHVRRNIESMDAAAYNSLIVNHYNLSMEFENAFHQEFSNKLRLELSPSVSDMNFVSGSSSTHVSNEDEESLQKHLSEGELTEMQTRTERPGCSENEEAISYYQANPNNDS*