AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG010971

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010971 MtrunA17_Chr2g0331131 96.890 836 6 1 1 816 1 836 0.0 1672
MsaG010971 MtrunA17_Chr8g0340411 87.694 837 77 5 1 816 1 832 0.0 1523
MsaG010971 MtrunA17_Chr3g0140351 80.622 836 132 6 3 816 8 835 0.0 1410
MsaG010971 MtrunA17_Chr2g0292391 67.268 831 245 8 10 816 9 836 0.0 1151
MsaG010971 MtrunA17_Chr4g0028991 65.024 832 257 7 10 816 24 846 0.0 1110
MsaG010971 MtrunA17_Chr2g0326731 61.511 834 277 10 12 816 21 839 0.0 1040
MsaG010971 MtrunA17_Chr2g0331581 21.887 530 315 18 15 477 143 640 3.98e-19 92.8
MsaG010971 MtrunA17_Chr8g0350321 22.037 481 294 17 203 651 375 806 6.83e-13 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG010971 AT1G52150.2 83.194 839 114 5 1 816 3 837 0.0 1455
MsaG010971 AT1G52150.1 83.433 839 111 6 1 816 3 836 0.0 1452
MsaG010971 AT1G52150.3 83.059 791 106 6 1 768 3 788 0.0 1354
MsaG010971 AT4G32880.1 77.174 828 163 8 10 816 11 833 0.0 1330
MsaG010971 AT2G34710.1 65.072 836 259 11 10 816 21 852 0.0 1098
MsaG010971 AT1G30490.1 63.278 836 267 12 10 816 17 841 0.0 1076
MsaG010971 AT5G60690.1 63.397 836 259 10 12 816 23 842 0.0 1073
MsaG010971 AT5G32670.1 64.865 74 26 0 283 356 37 110 1.37e-25 105
MsaG010971 AT1G79840.2 23.017 643 375 27 6 588 125 707 1.19e-21 101
MsaG010971 AT1G79840.3 23.017 643 375 27 6 588 38 620 1.29e-21 100
MsaG010971 AT1G79840.6 23.017 643 375 27 6 588 38 620 1.29e-21 100
MsaG010971 AT1G79840.1 23.017 643 375 27 6 588 96 678 1.87e-21 100
MsaG010971 AT1G79840.4 23.327 523 325 20 6 477 96 593 1.10e-20 97.8
MsaG010971 AT1G79840.5 23.885 381 237 12 6 345 96 464 5.65e-16 82.4
MsaG010971 AT1G05230.3 22.651 415 236 15 203 588 300 658 1.42e-11 68.6

Find 238 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACTAGTACCTTCTATTT+TGG 0.109247 2:+79954326 None:intergenic
CGTATCATTCCTCTTGATTC+TGG 0.153416 2:-79949116 MsaT010971.1:CDS
TTGCATCCCTTGGCGGATGC+TGG 0.201294 2:+79953658 None:intergenic
GGATCTCGGGTTGGAAATTA+TGG 0.220133 2:-79951147 MsaT010971.1:CDS
TTCGTCGTCAACAGCTTATT+CGG 0.220782 2:-79954399 MsaT010971.1:CDS
TTCATCACCTGACAAATTAA+TGG 0.260446 2:-79951832 MsaT010971.1:CDS
CGAGCAAGTGTTTGAGCTTC+AGG 0.260912 2:+79948683 None:intergenic
CGAATCACAGCTTGTATCTT+TGG 0.263868 2:+79953713 None:intergenic
GTTTCTGGAAGTCATTAAAT+TGG 0.266386 2:-79950995 MsaT010971.1:CDS
TCTCATTTGGTGTATGAAAA+TGG 0.275958 2:-79953887 MsaT010971.1:CDS
CATTGTTGATCATATGGATT+TGG 0.289894 2:-79952434 MsaT010971.1:CDS
TCAAGAGCTTCAACTTGTTC+AGG 0.292799 2:+79954457 None:intergenic
TCTAGGCATTATCGCGTCTT+CGG 0.303913 2:+79950954 None:intergenic
CTCCTTCACAAGGCCTTATC+AGG 0.319184 2:+79952473 None:intergenic
CATCCACATTGTTGATCATA+TGG 0.320194 2:-79952440 MsaT010971.1:CDS
ACAACTCTTTCATATGAAAT+AGG 0.321200 2:+79947670 None:intergenic
ATACTAACCGGCTCAGTCTT+TGG 0.332223 2:+79952019 None:intergenic
TTACCTTCATTCCAGGCATT+TGG 0.333732 2:+79953436 None:intergenic
GTTGGTCCTTGTAGTTTCTC+TGG 0.340294 2:-79951168 MsaT010971.1:CDS
TCAGGTCAAGGGTGCGATTT+GGG 0.342028 2:+79948950 None:intergenic
TGGCTCCTGCTCGAGACTTA+TGG 0.347028 2:-79952802 MsaT010971.1:CDS
GCTTGTGAATTCAAATTAGA+AGG 0.347776 2:+79948731 None:intergenic
GGTAAAGGTGTAATGGATAA+TGG 0.351251 2:-79954499 MsaT010971.1:CDS
AACCTAAACAGATCAAAGTT+TGG 0.353061 2:-79954351 MsaT010971.1:CDS
CTAGGCATTATCGCGTCTTC+GGG 0.356154 2:+79950955 None:intergenic
CTGACATCTGCCCTTGATAT+TGG 0.360765 2:-79948931 MsaT010971.1:intron
AATTAGATTACCTTCATTCC+AGG 0.362016 2:+79953429 None:intergenic
GCAAGACCATGTAGCATCTA+TGG 0.368008 2:-79948813 MsaT010971.1:CDS
CTTGTAGTTTCTCTGGATCT+CGG 0.369189 2:-79951161 MsaT010971.1:CDS
GTTGGATCAGCAACAGTTAT+AGG 0.370148 2:-79948661 MsaT010971.1:intron
AACCAAACTTTGATCTGTTT+AGG 0.380939 2:+79954349 None:intergenic
TTCAGACCCATTAATTTGTC+AGG 0.382426 2:+79951825 None:intergenic
GTCAGGTCAAGGGTGCGATT+TGG 0.385595 2:+79948949 None:intergenic
TGCATCCCTTGGCGGATGCT+GGG 0.387641 2:+79953659 None:intergenic
ATGGGTCTGAATCTTTCATT+TGG 0.392816 2:-79951813 MsaT010971.1:CDS
TTTGCTTTATGTTTGTCAAT+TGG 0.393364 2:-79947606 MsaT010971.1:CDS
TGCTGCACAGGAGGCATGCT+TGG 0.395041 2:+79952529 None:intergenic
TATACCTGTTGTATAATTAG+AGG 0.399547 2:+79947911 None:intergenic
CAGCCTGGTCCGGAATCTAT+TGG 0.406439 2:-79953206 MsaT010971.1:CDS
AAGTTTGGTTCCAAAATAGA+AGG 0.406689 2:-79954336 MsaT010971.1:intron
GATCTCGGGTTGGAAATTAT+GGG 0.407310 2:-79951146 MsaT010971.1:CDS
GCTATTATGTCTTGCAAAGA+TGG 0.408051 2:-79954520 None:intergenic
GAGTTTCTTTCAAAGGCTAC+TGG 0.411427 2:-79953477 MsaT010971.1:CDS
AAGGGTGCGATTTGGGTTGT+TGG 0.420594 2:+79948957 None:intergenic
ATTTCCTCTAATTATACAAC+AGG 0.421279 2:-79947915 MsaT010971.1:intron
GTATCATTCCTCTTGATTCT+GGG 0.423470 2:-79949115 MsaT010971.1:CDS
CCAAGGGATGCAAGTCCTGC+AGG 0.423738 2:-79953648 MsaT010971.1:CDS
CTGCATGTAAAGCAGCTCTA+TGG 0.428426 2:+79952931 None:intergenic
CAAGGTTGCAAGCTGTGAAT+AGG 0.428938 2:-79953970 MsaT010971.1:CDS
TCAGCAAAGGATGCATCAAT+TGG 0.431477 2:+79949167 None:intergenic
TATGGTTCCACCATTGGCTG+TGG 0.433473 2:+79952949 None:intergenic
CCAGCAGCACTTCGAGCACT+TGG 0.434472 2:-79952042 MsaT010971.1:CDS
TCATCACCTGACAAATTAAT+GGG 0.434535 2:-79951831 MsaT010971.1:CDS
GCAGAAACAAGTATCTCATT+TGG 0.436202 2:-79953900 MsaT010971.1:CDS
GTCAGACCACTGACCCTTGT+AGG 0.441681 2:+79953116 None:intergenic
TCGTCGTCAACAGCTTATTC+GGG 0.442226 2:-79954398 MsaT010971.1:CDS
CCAGGCAGGGCTTGACATGC+TGG 0.442745 2:-79948017 MsaT010971.1:CDS
CCAAGTGCTCGAAGTGCTGC+TGG 0.443748 2:+79952042 None:intergenic
ATTCCGAGCACAGAATCTTT+CGG 0.444438 2:+79947936 None:intergenic
CCAGCATGTCAAGCCCTGCC+TGG 0.449681 2:+79948017 None:intergenic
ATGATTTGTGCAGCCTGGTC+CGG 0.450366 2:-79953216 MsaT010971.1:intron
CCATTCTACTAAGGTTTCTA+CGG 0.450972 2:-79951251 MsaT010971.1:CDS
CTCAGTCTAATGTCACTGGA+TGG 0.451187 2:-79952074 MsaT010971.1:CDS
TGCCTCCGGCCATTCTACTA+AGG 0.451669 2:-79951260 MsaT010971.1:CDS
GCAAGAGCATGTGGCCTAGT+GGG 0.454131 2:-79953146 MsaT010971.1:CDS
TTGTAGTTTCTCTGGATCTC+GGG 0.456205 2:-79951160 MsaT010971.1:CDS
TGCCCAGTGGGTACCTGATA+AGG 0.456662 2:-79952486 MsaT010971.1:CDS
CATGCTCTTGCCGCCACACC+AGG 0.457174 2:+79953158 None:intergenic
CAAAGATGGTAAAGGTGTAA+TGG 0.457615 2:-79954506 MsaT010971.1:CDS
GGTTTCAATGAGGCACTCAA+TGG 0.458902 2:-79951921 MsaT010971.1:CDS
TATATCCTGCAGAGCAACTA+AGG 0.459043 2:+79947988 None:intergenic
CTGAATCTTTCATTTGGCAA+TGG 0.460308 2:-79951807 MsaT010971.1:CDS
CAGCTCTATGGTTCCACCAT+TGG 0.460705 2:+79952943 None:intergenic
GCAGGACTTGCATCCCTTGG+CGG 0.462034 2:+79953651 None:intergenic
ACCACGAGGGCCGATCTTTG+AGG 0.463233 2:+79953011 None:intergenic
CATGTCAAGCCCTGCCTGGT+TGG 0.463534 2:+79948021 None:intergenic
TGAATTCACAAGCTGGGCTC+AGG 0.465475 2:-79948721 MsaT010971.1:CDS
GGTCTTTGTCTCTCAAGCAT+GGG 0.471448 2:-79947697 MsaT010971.1:CDS
GGCAAGAGCATGTGGCCTAG+TGG 0.474488 2:-79953147 MsaT010971.1:CDS
TTATCGCGTCTTCGGGAGAA+TGG 0.474560 2:+79950962 None:intergenic
AGCTGGGCTCAGGACACCAC+TGG 0.476439 2:-79948711 MsaT010971.1:CDS
CTTCATGTGAAATCTGCCTT+AGG 0.477909 2:+79952100 None:intergenic
CAAAGATACAAGCTGTGATT+CGG 0.478150 2:-79953712 MsaT010971.1:CDS
TGTTGATCATATGGATTTGG+AGG 0.478929 2:-79952431 MsaT010971.1:intron
GTTGAGTGGGTCCAAATGCC+TGG 0.479281 2:-79953447 MsaT010971.1:CDS
TCTAATTTGAATTCACAAGC+TGG 0.482009 2:-79948728 MsaT010971.1:CDS
TTTAGCAGAGTTTCTTTCAA+AGG 0.484145 2:-79953484 MsaT010971.1:CDS
CTGGAAGTCATTAAATTGGA+AGG 0.484276 2:-79950991 MsaT010971.1:CDS
AGCAGAGAAAAGAGGCTTCA+AGG 0.487168 2:-79953988 MsaT010971.1:CDS
ACTACAAGGACCAACTTTGA+TGG 0.487918 2:+79951176 None:intergenic
GGTTCAATATGAGAGAGAAT+AGG 0.490102 2:+79954370 None:intergenic
CGACGAATACTACTTGGCTT+AGG 0.490184 2:+79954415 None:intergenic
CCTGCAGGACTTGCATCCCT+TGG 0.490435 2:+79953648 None:intergenic
CTAATTTGAATTCACAAGCT+GGG 0.492834 2:-79948727 MsaT010971.1:CDS
GACTACCTTAGTTGCTCTGC+AGG 0.493839 2:-79947993 MsaT010971.1:CDS
CAGTCTAATGTCACTGGATG+GGG 0.495074 2:-79952072 MsaT010971.1:CDS
CAATATCAAGGGCAGATGTC+AGG 0.496738 2:+79948932 None:intergenic
AAAGTGCCAATGCTACCCTT+TGG 0.497966 2:+79948756 None:intergenic
CGAAATCCAGAAGGCAGAAG+GGG 0.500490 2:+79949137 None:intergenic
ATTCCAATAGATTCCGGACC+AGG 0.502970 2:+79953203 None:intergenic
TGTTGACGACGAATACTACT+TGG 0.503246 2:+79954409 None:intergenic
GTTTCTCAGTCTAATGTCAC+TGG 0.504256 2:-79952078 MsaT010971.1:CDS
AATACTCTGCTGCACTCTAA+AGG 0.505703 2:-79948417 MsaT010971.1:intron
GTCTGTCCAAAGGGTAGCAT+TGG 0.507607 2:-79948762 MsaT010971.1:CDS
GGTCCAAATGCCTGGAATGA+AGG 0.509086 2:-79953439 MsaT010971.1:intron
GGAATCGTTGCGATTTCTCA+TGG 0.509332 2:-79953185 MsaT010971.1:CDS
AGAGACAAAGACCACCTTGA+AGG 0.512267 2:+79947707 None:intergenic
CTTTGCCTAGAATGTGCCTC+CGG 0.513530 2:-79951274 MsaT010971.1:intron
GCAACGATTCCAATAGATTC+CGG 0.514017 2:+79953197 None:intergenic
TGTCCGCGGGACCAATATCA+AGG 0.514462 2:+79948920 None:intergenic
TCAGTCTAATGTCACTGGAT+GGG 0.514519 2:-79952073 MsaT010971.1:CDS
GCTCTGACAAAATGCTGCAC+AGG 0.515515 2:+79952517 None:intergenic
TACTGGAACTGCTGTTGAGT+GGG 0.516900 2:-79953460 MsaT010971.1:CDS
TAGAGTGGACGCAGTACCTC+GGG 0.517204 2:+79952315 None:intergenic
TGGTCAATGATTGGCAATGA+TGG 0.517457 2:-79951882 MsaT010971.1:CDS
TATCGCGTCTTCGGGAGAAT+GGG 0.517540 2:+79950963 None:intergenic
CATATGAAAGAGTTGTGGCT+TGG 0.517555 2:-79947663 MsaT010971.1:CDS
CATTATATCGTCTGTCCAAA+GGG 0.517690 2:-79948771 MsaT010971.1:CDS
GTTTCAATGAGGCACTCAAT+GGG 0.519441 2:-79951920 MsaT010971.1:CDS
TCAGCTGCTGCCATCAAAGT+TGG 0.520575 2:-79951186 MsaT010971.1:CDS
CTATGAATCATCAACAATGC+TGG 0.521829 2:-79952295 MsaT010971.1:CDS
CCTCCTCGTGCTCAATAGTG+TGG 0.526283 2:+79951100 None:intergenic
AGTTTCTCTGGATCTCGGGT+TGG 0.526941 2:-79951156 MsaT010971.1:CDS
AACAAGTTGAAGCTCTTGAA+AGG 0.527652 2:-79954453 MsaT010971.1:CDS
CGAGCAGGAGCCAATGTAGT+TGG 0.527668 2:+79952812 None:intergenic
GCGATTTCTCATGGTTGCCC+TGG 0.528908 2:-79953176 MsaT010971.1:CDS
TGGTCTTTGTCTCTCAAGCA+TGG 0.530702 2:-79947698 MsaT010971.1:CDS
GCTATATGCGCCAACTACAT+TGG 0.531502 2:-79952822 MsaT010971.1:CDS
GCATTATATCGTCTGTCCAA+AGG 0.531571 2:-79948772 MsaT010971.1:CDS
ATGATCAACAATGTGGATGA+TGG 0.533950 2:+79952444 None:intergenic
CATAGAGCTGCTTTACATGC+AGG 0.535587 2:-79952930 MsaT010971.1:intron
TGCTAAAGTTTCCTCTGCAA+TGG 0.535889 2:+79953500 None:intergenic
TGGCCGAAAGATTCTGTGCT+CGG 0.536672 2:-79947939 MsaT010971.1:CDS
CTCAATGGGTTTACTGATGA+GGG 0.537693 2:-79951906 MsaT010971.1:CDS
CCGTAGAAACCTTAGTAGAA+TGG 0.538900 2:+79951251 None:intergenic
TCCAGGCCCTACGTCACCTA+AGG 0.539524 2:-79952116 MsaT010971.1:intron
TGTTTGTTAATATAGATGTA+GGG 0.540006 2:-79954014 MsaT010971.1:intron
TGCTATGAATAAGCTGCTGA+TGG 0.540866 2:-79953939 MsaT010971.1:CDS
TGCTGATGGAAGAGAATGAT+AGG 0.540990 2:-79953925 MsaT010971.1:CDS
TGCTCTGCAGGATATAGCAT+TGG 0.542984 2:-79947981 MsaT010971.1:CDS
AGGCCTTATCAGGTACCCAC+TGG 0.544746 2:+79952483 None:intergenic
TGCAAAAGAAACACTTCTCT+AGG 0.547856 2:+79950937 None:intergenic
GATGAGGGATGGTCAATGAT+TGG 0.547940 2:-79951891 MsaT010971.1:CDS
CATTCTACTAAGGTTTCTAC+GGG 0.549529 2:-79951250 MsaT010971.1:CDS
CTGATAAGGCCTTGTGAAGG+AGG 0.549792 2:-79952472 MsaT010971.1:CDS
GGGCTAGAGGAAGAATTACT+TGG 0.550060 2:+79951121 None:intergenic
CAAGAGCATGTGGCCTAGTG+GGG 0.553313 2:-79953145 MsaT010971.1:CDS
AGCACAGAATCTTTCGGCCA+TGG 0.554959 2:+79947942 None:intergenic
AACGTGCTTCCCACAGCCAA+TGG 0.555580 2:-79952959 MsaT010971.1:CDS
AGCAAATATAAGTTCAGCAC+AGG 0.556260 2:+79949190 None:intergenic
AACAACCATAAGTCTCGAGC+AGG 0.556394 2:+79952797 None:intergenic
TACGAAATCCAGAAGGCAGA+AGG 0.557452 2:+79949135 None:intergenic
AAGCTCAAACACTTGCTCGT+TGG 0.557927 2:-79948679 MsaT010971.1:CDS
ATGGTTCCACCATTGGCTGT+GGG 0.559192 2:+79952950 None:intergenic
ATGTCTTGCAAAGATGGTAA+AGG 0.562359 2:-79954514 MsaT010971.1:CDS
TGCTGGCAGGGGTTTCAATG+AGG 0.567674 2:-79951931 MsaT010971.1:intron
TTCTCATGGTTGCCCTGGTG+TGG 0.567875 2:-79953171 MsaT010971.1:CDS
CATTCCTCTTGATTCTGGGA+AGG 0.568169 2:-79949111 MsaT010971.1:intron
ATTGTTGATGATTCATAGAG+TGG 0.569522 2:+79952300 None:intergenic
GTCTTTGTCTCTCAAGCATG+GGG 0.569931 2:-79947696 MsaT010971.1:CDS
ACGAAATCCAGAAGGCAGAA+GGG 0.570215 2:+79949136 None:intergenic
AGAAACCTTAGTAGAATGGC+CGG 0.570667 2:+79951255 None:intergenic
GAGCTGAGATGCTGCCCAGT+GGG 0.572763 2:-79952498 MsaT010971.1:CDS
TCATGGTTGCCCTGGTGTGG+CGG 0.573680 2:-79953168 MsaT010971.1:CDS
TTGATAGCTCTGCAGTGGAA+TGG 0.575064 2:-79949231 MsaT010971.1:intron
TTGCAGAAATCCTCAAAGAT+CGG 0.576829 2:-79953021 MsaT010971.1:CDS
CTGACAAAATGCTGCACAGG+AGG 0.577091 2:+79952520 None:intergenic
ACTCAATGGGTTTACTGATG+AGG 0.577528 2:-79951907 MsaT010971.1:CDS
TGCTCAATAGTGTGGGCTAG+AGG 0.577663 2:+79951108 None:intergenic
CAAGGGCAGATGTCAGGTCA+AGG 0.578879 2:+79948938 None:intergenic
TAGGGAGAAGCAGAGAAAAG+AGG 0.579898 2:-79953996 MsaT010971.1:CDS
CATATTCCCACTAACCCTGC+AGG 0.581342 2:+79953633 None:intergenic
CTGGTTGGCAAAAGTGAACA+CGG 0.582446 2:+79948036 None:intergenic
CGAGATCCAGAGAAACTACA+AGG 0.585194 2:+79951162 None:intergenic
CTCCTCGTGCTCAATAGTGT+GGG 0.587564 2:+79951101 None:intergenic
ATAAGGCCTTGTGAAGGAGG+TGG 0.587584 2:-79952469 MsaT010971.1:CDS
AGTACCTCGGGAACACTCCA+TGG 0.589994 2:+79952327 None:intergenic
TGTGATTCGGCGGTGACTAG+TGG 0.591394 2:-79953699 MsaT010971.1:CDS
ATAGAGTGGACGCAGTACCT+CGG 0.591468 2:+79952314 None:intergenic
TATTTCATATGAAAGAGTTG+TGG 0.591556 2:-79947668 MsaT010971.1:CDS
TAGTGGGGCTAGAACCTACA+AGG 0.592552 2:-79953130 MsaT010971.1:CDS
AAGACAGCATTACTGACAGA+TGG 0.593832 2:+79951780 None:intergenic
GACAGCCCAGCATCCGCCAA+GGG 0.598760 2:-79953664 MsaT010971.1:CDS
TTGTAGGTTCTAGCCCCACT+AGG 0.599076 2:+79953132 None:intergenic
TGAAAGAGTTGTGGCTTGGA+AGG 0.599817 2:-79947659 MsaT010971.1:CDS
TCCTCAAAGATCGGCCCTCG+TGG 0.600087 2:-79953012 MsaT010971.1:CDS
ATCTCGGGTTGGAAATTATG+GGG 0.600717 2:-79951145 MsaT010971.1:CDS
TGCATCAGTGTGATGCCACA+AGG 0.601694 2:+79948439 None:intergenic
GTATCGTGATTGCCGAGCTG+TGG 0.604463 2:-79952990 MsaT010971.1:CDS
CTACTTAAATCTAATAACGA+GGG 0.605013 2:-79948482 MsaT010971.1:CDS
AAATCTGCCTTAGGTGACGT+AGG 0.611020 2:+79952109 None:intergenic
TGACAGCCCAGCATCCGCCA+AGG 0.614338 2:-79953665 MsaT010971.1:CDS
CAAGGGATGCAAGTCCTGCA+GGG 0.614855 2:-79953647 MsaT010971.1:intron
GCCGTGCCATAGATGCTACA+TGG 0.614931 2:+79948807 None:intergenic
ACGGGAGCATAGATCAGAGT+GGG 0.615662 2:-79951232 MsaT010971.1:CDS
GGCCTTATCAGGTACCCACT+GGG 0.617608 2:+79952484 None:intergenic
GTGCTTCCCACAGCCAATGG+TGG 0.620635 2:-79952956 MsaT010971.1:CDS
GAGAAAATATTTGATGACCA+TGG 0.622292 2:-79947959 MsaT010971.1:CDS
TCCTGTTTCAGATATGTGAG+AGG 0.625263 2:-79952576 MsaT010971.1:intron
ACCATGTAGCATCTATGGCA+CGG 0.626691 2:-79948808 MsaT010971.1:CDS
AATCTGCCTTAGGTGACGTA+GGG 0.627907 2:+79952110 None:intergenic
ATGCTCTTGCCGCCACACCA+GGG 0.629106 2:+79953159 None:intergenic
CGTTCAGAATATCCACAGCT+CGG 0.629690 2:+79952978 None:intergenic
AAGGGCAGATGTCAGGTCAA+GGG 0.631428 2:+79948939 None:intergenic
CTACTGGAACTGCTGTTGAG+TGG 0.637529 2:-79953461 MsaT010971.1:CDS
ATGGAGCCACCTCCTTCACA+AGG 0.638572 2:+79952463 None:intergenic
AGATACAAGCTGTGATTCGG+CGG 0.639452 2:-79953709 MsaT010971.1:CDS
GCTGGGCTCAGGACACCACT+GGG 0.640667 2:-79948710 MsaT010971.1:CDS
GGTGTGGCGGCAAGAGCATG+TGG 0.641036 2:-79953155 MsaT010971.1:CDS
ATGGGTTTACTGATGAGGGA+TGG 0.641840 2:-79951902 MsaT010971.1:CDS
TACCTGATAAGGCCTTGTGA+AGG 0.644402 2:-79952475 MsaT010971.1:CDS
GAAATCCAGAAGGCAGAAGG+GGG 0.645694 2:+79949138 None:intergenic
CACTTGGCCAAAGACTGAGC+CGG 0.650113 2:-79952026 MsaT010971.1:intron
TTAGCCATGGAGTGTTCCCG+AGG 0.654050 2:-79952331 MsaT010971.1:intron
AATCCATATGATCAACAATG+TGG 0.655570 2:+79952437 None:intergenic
GTCCGCGGGACCAATATCAA+GGG 0.657432 2:+79948921 None:intergenic
TACTTAAATCTAATAACGAG+GGG 0.658209 2:-79948481 MsaT010971.1:CDS
TGGTTGGCAAAAGTGAACAC+GGG 0.662661 2:+79948037 None:intergenic
CTGACCTTCCCAGAATCAAG+AGG 0.668957 2:+79949107 None:intergenic
AATCATTACTCAAGTCCTTG+TGG 0.672589 2:-79948454 MsaT010971.1:CDS
GGAATGGATGAGAATGCTGT+TGG 0.673638 2:-79949215 MsaT010971.1:CDS
AGCCCACACTATTGAGCACG+AGG 0.673679 2:-79951103 MsaT010971.1:CDS
GGCTCAGAAGACAACAATGG+TGG 0.673814 2:-79952274 MsaT010971.1:intron
TTTAGAAGATGGAAGCCTAG+TGG 0.677986 2:-79952759 MsaT010971.1:intron
GGAATGATACGAAATCCAGA+AGG 0.683077 2:+79949128 None:intergenic
AGAGTGGGCAGACAACAACA+TGG 0.684198 2:-79951217 MsaT010971.1:CDS
TGAGCTTCAGGAGTACCCAG+TGG 0.684885 2:+79948695 None:intergenic
GCTACTTAAATCTAATAACG+AGG 0.694840 2:-79948483 MsaT010971.1:CDS
TCGGCAATCACGATACCACG+AGG 0.695617 2:+79952997 None:intergenic
AAATCCAGAAGGCAGAAGGG+GGG 0.697502 2:+79949139 None:intergenic
TACGGGAGCATAGATCAGAG+TGG 0.712778 2:-79951233 MsaT010971.1:CDS
TCTCGGGTTGGAAATTATGG+GGG 0.713766 2:-79951144 MsaT010971.1:CDS
GCTGGCTCAGAAGACAACAA+TGG 0.718261 2:-79952277 MsaT010971.1:CDS
CGGCAATCACGATACCACGA+GGG 0.718468 2:+79952998 None:intergenic
AACCTTAGTAGAATGGCCGG+AGG 0.718736 2:+79951258 None:intergenic
CCACACTATTGAGCACGAGG+AGG 0.733047 2:-79951100 MsaT010971.1:intron
AGAGCTGAGATGCTGCCCAG+TGG 0.741837 2:-79952499 MsaT010971.1:CDS
AGTGGGGCTAGAACCTACAA+GGG 0.773703 2:-79953129 MsaT010971.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 79947594 79954536 79947594 ID=MsaG010971
Chr2 mRNA 79947594 79954536 79947594 ID=MsaT010971.1;Parent=MsaG010971
Chr2 exon 79947594 79947740 79947594 ID=MsaT010971.1.exon19;Parent=MsaT010971.1
Chr2 CDS 79947594 79947740 79947594 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79947916 79948065 79947916 ID=MsaT010971.1.exon18;Parent=MsaT010971.1
Chr2 CDS 79947916 79948065 79947916 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79948418 79948523 79948418 ID=MsaT010971.1.exon17;Parent=MsaT010971.1
Chr2 CDS 79948418 79948523 79948418 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79948662 79948875 79948662 ID=MsaT010971.1.exon16;Parent=MsaT010971.1
Chr2 CDS 79948662 79948875 79948662 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79948932 79948986 79948932 ID=MsaT010971.1.exon15;Parent=MsaT010971.1
Chr2 CDS 79948932 79948986 79948932 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79949112 79949246 79949112 ID=MsaT010971.1.exon14;Parent=MsaT010971.1
Chr2 CDS 79949112 79949246 79949112 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79950936 79951016 79950936 ID=MsaT010971.1.exon13;Parent=MsaT010971.1
Chr2 CDS 79950936 79951016 79950936 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79951101 79951286 79951101 ID=MsaT010971.1.exon12;Parent=MsaT010971.1
Chr2 CDS 79951101 79951286 79951101 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79951752 79951944 79951752 ID=MsaT010971.1.exon11;Parent=MsaT010971.1
Chr2 CDS 79951752 79951944 79951752 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79952027 79952133 79952027 ID=MsaT010971.1.exon10;Parent=MsaT010971.1
Chr2 CDS 79952027 79952133 79952027 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79952275 79952349 79952275 ID=MsaT010971.1.exon9;Parent=MsaT010971.1
Chr2 CDS 79952275 79952349 79952275 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79952432 79952587 79952432 ID=MsaT010971.1.exon8;Parent=MsaT010971.1
Chr2 CDS 79952432 79952587 79952432 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79952760 79952843 79952760 ID=MsaT010971.1.exon7;Parent=MsaT010971.1
Chr2 CDS 79952760 79952843 79952760 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79952931 79953044 79952931 ID=MsaT010971.1.exon6;Parent=MsaT010971.1
Chr2 CDS 79952931 79953044 79952931 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79953130 79953225 79953130 ID=MsaT010971.1.exon5;Parent=MsaT010971.1
Chr2 CDS 79953130 79953225 79953130 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79953440 79953530 79953440 ID=MsaT010971.1.exon4;Parent=MsaT010971.1
Chr2 CDS 79953440 79953530 79953440 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79953648 79953748 79953648 ID=MsaT010971.1.exon3;Parent=MsaT010971.1
Chr2 CDS 79953648 79953748 79953648 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79953862 79954021 79953862 ID=MsaT010971.1.exon2;Parent=MsaT010971.1
Chr2 CDS 79953862 79954021 79953862 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Chr2 exon 79954337 79954536 79954337 ID=MsaT010971.1.exon1;Parent=MsaT010971.1
Chr2 CDS 79954337 79954536 79954337 ID=cds.MsaT010971.1;Parent=MsaT010971.1
Gene Sequence

>MsaT010971.1

ATGTCTTGCAAAGATGGTAAAGGTGTAATGGATAATGGAAAATACGTTCGTTATACACCTGAACAAGTTGAAGCTCTTGAAAGGCTTTATCATGATTGTCCTAAGCCAAGTAGTATTCGTCGTCAACAGCTTATTCGGGAGTGTCCTATTCTCTCTCATATTGAACCTAAACAGATCAAAGTTTGGTTCCAAAATAGAAGATGTAGGGAGAAGCAGAGAAAAGAGGCTTCAAGGTTGCAAGCTGTGAATAGGAAGTTGAGTGCTATGAATAAGCTGCTGATGGAAGAGAATGATAGGTTGCAGAAACAAGTATCTCATTTGGTGTATGAAAATGGCTACTTTCGCCAACATACTCAAAATACTAATCTTGCAACCAAAGATACAAGCTGTGATTCGGCGGTGACTAGTGGTCAACGCAGTTTGACAGCCCAGCATCCGCCAAGGGATGCAAGTCCTGCAGGGCTTTTGTCCATTGCAGAGGAAACTTTAGCAGAGTTTCTTTCAAAGGCTACTGGAACTGCTGTTGAGTGGGTCCAAATGCCTGGAATGAAGCCTGGTCCGGAATCTATTGGAATCGTTGCGATTTCTCATGGTTGCCCTGGTGTGGCGGCAAGAGCATGTGGCCTAGTGGGGCTAGAACCTACAAGGGTTGCAGAAATCCTCAAAGATCGGCCCTCGTGGTATCGTGATTGCCGAGCTGTGGATATTCTGAACGTGCTTCCCACAGCCAATGGTGGAACCATAGAGCTGCTTTACATGCAGCTATATGCGCCAACTACATTGGCTCCTGCTCGAGACTTATGGTTGTTGCGCTACACATCTGTTTTAGAAGATGGAAGCCTAGTGATATGTGAGAGGTCTCTTAAAAACACACAAAATGGTCCAAGCATGCCTCCTGTGCAGCATTTTGTCAGAGCTGAGATGCTGCCCAGTGGGTACCTGATAAGGCCTTGTGAAGGAGGTGGCTCCATCATCCACATTGTTGATCATATGGATTTGGAGCCATGGAGTGTTCCCGAGGTACTGCGTCCACTCTATGAATCATCAACAATGCTGGCTCAGAAGACAACAATGGTGGCCCTACGTCACCTAAGGCAGATTTCACATGAAGTTTCTCAGTCTAATGTCACTGGATGGGGTAGACGACCAGCAGCACTTCGAGCACTTGGCCAAAGACTGAGCCGGGGTTTCAATGAGGCACTCAATGGGTTTACTGATGAGGGATGGTCAATGATTGGCAATGATGGTGTTGACGATGTTACTATTCTAGTGAATTCATCACCTGACAAATTAATGGGTCTGAATCTTTCATTTGGCAATGGATTTCCATCTGTCAGTAATGCTGTCTTATGTGCTAAAGCTTCAATGCTGTTACAAAATGTGCCTCCGGCCATTCTACTAAGGTTTCTACGGGAGCATAGATCAGAGTGGGCAGACAACAACATGGATGCTTACTCAGCTGCTGCCATCAAAGTTGGTCCTTGTAGTTTCTCTGGATCTCGGGTTGGAAATTATGGGGGCCAAGTAATTCTTCCTCTAGCCCACACTATTGAGCACGAGGAGTTTCTGGAAGTCATTAAATTGGAAGGAATCGCCCATTCTCCCGAAGACGCGATAATGCCTAGAGAAGTGTTTCTTTTGCAACTCTGCAGTGGAATGGATGAGAATGCTGTTGGTACCTGTGCTGAACTTATATTTGCTCCAATTGATGCATCCTTTGCTGATGATGCCCCCCTTCTGCCTTCTGGATTTCGTATCATTCCTCTTGATTCTGGGAAGGAAGTGGCCAACAACCCAAATCGCACCCTTGACCTGACATCTGCCCTTGATATTGTGATGACAATAGCATTTGAATTTGCTTTTGAAAGTCATATGCAAGACCATGTAGCATCTATGGCACGGCAATATGTTCGCAGCATTATATCGTCTGTCCAAAGGGTAGCATTGGCACTTTCACCTTCTAATTTGAATTCACAAGCTGGGCTCAGGACACCACTGGGTACTCCTGAAGCTCAAACACTTGCTCGTTGGATCAGCAACAGTTATAGGTGCTTCTTGGGTACTGAGCTACTTAAATCTAATAACGAGGGGAGCGAATCATTACTCAAGTCCTTGTGGCATCACACTGATGCAATACTCTGCTGCACTCTAAAGGCGTTGCCCGTGTTCACTTTTGCCAACCAGGCAGGGCTTGACATGCTGGAGACTACCTTAGTTGCTCTGCAGGATATAGCATTGGAGAAAATATTTGATGACCATGGCCGAAAGATTCTGTGCTCGGAATTTCCTCTAATTATACAACAGGGTTTTGCGTGCCTTCAAGGTGGTCTTTGTCTCTCAAGCATGGGGAGACCTATTTCATATGAAAGAGTTGTGGCTTGGAAGGTGCTGAATGAAGAAGAGAATGCTCATTGTATTTGCTTTATGTTTGTCAATTGGTCTTTTGTTTGA
Protein sequence

>MsaT010971.1

MSCKDGKGVMDNGKYVRYTPEQVEALERLYHDCPKPSSIRRQQLIRECPILSHIEPKQIKVWFQNRRCREKQRKEASRLQAVNRKLSAMNKLLMEENDRLQKQVSHLVYENGYFRQHTQNTNLATKDTSCDSAVTSGQRSLTAQHPPRDASPAGLLSIAEETLAEFLSKATGTAVEWVQMPGMKPGPESIGIVAISHGCPGVAARACGLVGLEPTRVAEILKDRPSWYRDCRAVDILNVLPTANGGTIELLYMQLYAPTTLAPARDLWLLRYTSVLEDGSLVICERSLKNTQNGPSMPPVQHFVRAEMLPSGYLIRPCEGGGSIIHIVDHMDLEPWSVPEVLRPLYESSTMLAQKTTMVALRHLRQISHEVSQSNVTGWGRRPAALRALGQRLSRGFNEALNGFTDEGWSMIGNDGVDDVTILVNSSPDKLMGLNLSFGNGFPSVSNAVLCAKASMLLQNVPPAILLRFLREHRSEWADNNMDAYSAAAIKVGPCSFSGSRVGNYGGQVILPLAHTIEHEEFLEVIKLEGIAHSPEDAIMPREVFLLQLCSGMDENAVGTCAELIFAPIDASFADDAPLLPSGFRIIPLDSGKEVANNPNRTLDLTSALDIVMTIAFEFAFESHMQDHVASMARQYVRSIISSVQRVALALSPSNLNSQAGLRTPLGTPEAQTLARWISNSYRCFLGTELLKSNNEGSESLLKSLWHHTDAILCCTLKALPVFTFANQAGLDMLETTLVALQDIALEKIFDDHGRKILCSEFPLIIQQGFACLQGGLCLSSMGRPISYERVVAWKVLNEEENAHCICFMFVNWSFV*