Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011003 | XP_024632959.1 | 73.391 | 233 | 57 | 3 | 1 | 232 | 1 | 229 | 1.75e-97 | 304 |
| MsaG011003 | XP_024632959.1 | 79.217 | 332 | 38 | 5 | 95 | 397 | 1 | 330 | 2.36e-171 | 492 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011003 | sp|Q39092|ZAT1_ARATH | 27.132 | 258 | 95 | 8 | 41 | 232 | 1 | 231 | 6.44e-13 | 72.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011003 | tr|A0A396JGH2|A0A396JGH2_MEDTR | 73.391 | 233 | 57 | 3 | 1 | 232 | 1 | 229 | 8.37e-98 | 304 |
| MsaG011003 | tr|A0A396JGH2|A0A396JGH2_MEDTR | 79.217 | 332 | 38 | 5 | 95 | 397 | 1 | 330 | 1.13e-171 | 492 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG011003 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011003 | MtrunA17_Chr2g0331601 | 79.217 | 332 | 38 | 5 | 95 | 397 | 1 | 330 | 2.17e-175 | 492 |
| MsaG011003 | MtrunA17_Chr2g0331601 | 73.391 | 233 | 57 | 3 | 1 | 232 | 1 | 229 | 1.61e-101 | 304 |
| MsaG011003 | MtrunA17_Chr6g0481281 | 40.418 | 287 | 92 | 7 | 180 | 397 | 30 | 306 | 4.76e-53 | 178 |
| MsaG011003 | MtrunA17_Chr6g0481281 | 42.748 | 131 | 52 | 5 | 109 | 233 | 30 | 143 | 8.37e-18 | 83.6 |
| MsaG011003 | MtrunA17_Chr6g0481281 | 62.319 | 69 | 21 | 2 | 11 | 75 | 27 | 94 | 9.04e-17 | 80.5 |
| MsaG011003 | MtrunA17_Chr5g0448221 | 31.429 | 175 | 80 | 5 | 201 | 355 | 55 | 209 | 5.02e-14 | 71.6 |
| MsaG011003 | MtrunA17_Chr5g0425411 | 25.957 | 235 | 135 | 7 | 8 | 233 | 3 | 207 | 3.24e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011003 | AT1G02030.1 | 27.132 | 258 | 95 | 8 | 41 | 232 | 1 | 231 | 6.55e-14 | 72.0 |
| MsaG011003 | AT5G56200.1 | 38.542 | 96 | 45 | 2 | 210 | 305 | 79 | 160 | 5.49e-13 | 70.9 |
| MsaG011003 | AT5G43170.1 | 29.114 | 158 | 80 | 2 | 80 | 233 | 12 | 141 | 5.90e-12 | 64.7 |
| MsaG011003 | AT2G17180.1 | 27.083 | 192 | 121 | 4 | 44 | 235 | 64 | 236 | 1.08e-11 | 65.5 |
| MsaG011003 | AT2G26940.1 | 37.143 | 105 | 61 | 1 | 210 | 309 | 49 | 153 | 1.24e-11 | 65.5 |
| MsaG011003 | AT3G19580.1 | 33.684 | 95 | 51 | 2 | 139 | 233 | 106 | 188 | 3.73e-11 | 63.9 |
| MsaG011003 | AT3G19580.2 | 33.684 | 95 | 51 | 2 | 139 | 233 | 106 | 188 | 3.73e-11 | 63.9 |
| MsaG011003 | AT3G60580.1 | 28.358 | 134 | 76 | 3 | 139 | 272 | 173 | 286 | 7.05e-11 | 63.2 |
Find 73 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATCTCTCTTCTGCCATTT+TGG | 0.136557 | 2:-80335883 | None:intergenic |
| CAATACAAGGTAGTTATAAT+AGG | 0.224337 | 2:+80335167 | MsaT011003.1:CDS |
| CAATACAAGGTAGTACTAAT+AGG | 0.256706 | 2:+80335662 | MsaT011003.1:CDS |
| GTGATTTATCTTTGCCAAAA+TGG | 0.304193 | 2:+80335869 | MsaT011003.1:CDS |
| GTCCTTTAAAATCTCTATTC+CGG | 0.308881 | 2:-80335804 | None:intergenic |
| CATGATGAAGTTTCTGCTTA+AGG | 0.329845 | 2:-80335399 | None:intergenic |
| GTGACCATACAAAGCATTCA+TGG | 0.407800 | 2:-80335770 | None:intergenic |
| CCAACCATGAATGCTTTGTA+TGG | 0.410676 | 2:+80335766 | MsaT011003.1:CDS |
| AATACAAGGTAGTACTAATA+GGG | 0.424373 | 2:+80335663 | MsaT011003.1:CDS |
| TACTACCTTGTATTGAGAAA+GGG | 0.428907 | 2:-80335654 | None:intergenic |
| AATACAAGGTAGTTATAATA+GGG | 0.431054 | 2:+80335168 | MsaT011003.1:CDS |
| CACATCAGTGCAATGTTTGT+GGG | 0.435235 | 2:+80335044 | MsaT011003.1:CDS |
| ACACATCAGTGCAATGTTTG+TGG | 0.442286 | 2:+80335538 | MsaT011003.1:CDS |
| TTTAGCAATGGAAAAGCATT+GGG | 0.444822 | 2:+80335073 | MsaT011003.1:CDS |
| TCACATCAGTGCAATGTTTG+TGG | 0.451639 | 2:+80335043 | MsaT011003.1:CDS |
| GTACTACCTTGTATTGAGAA+AGG | 0.452759 | 2:-80335655 | None:intergenic |
| ACACATCAATGCAATGTTTG+TGG | 0.453484 | 2:+80335325 | MsaT011003.1:CDS |
| TTTAGTAACGGAAAAGCATT+GGG | 0.453684 | 2:+80335568 | MsaT011003.1:CDS |
| ATTCGGAAAAGAGGATGAAA+AGG | 0.459648 | 2:+80334957 | MsaT011003.1:CDS |
| TGAGAAAACCTATGAAGATT+CGG | 0.461549 | 2:+80334940 | MsaT011003.1:CDS |
| TTTAGCAACGGAAAAGCATT+GGG | 0.462037 | 2:+80335355 | MsaT011003.1:CDS |
| TGTGTTGGTGAAACATCAAA+TGG | 0.466991 | 2:+80335979 | MsaT011003.1:CDS |
| AGTCAGAAAAGAGGGTGAAA+AGG | 0.473192 | 2:+80335452 | MsaT011003.1:CDS |
| GAATGAGTCGTATGTTATAG+AGG | 0.473597 | 2:+80336080 | MsaT011003.1:CDS |
| GATGATGAAGAAGAAATTGA+TGG | 0.474180 | 2:+80335703 | MsaT011003.1:CDS |
| AGAAGAGGTAGAAAGTGTAT+TGG | 0.483023 | 2:+80335904 | MsaT011003.1:CDS |
| AGTCTGAAAAGAGGATAAAG+AGG | 0.489348 | 2:+80335239 | MsaT011003.1:CDS |
| AGTGATAAGCAGCCAAAGAA+AGG | 0.491245 | 2:+80336039 | MsaT011003.1:CDS |
| TTTGATAAAGATAGTAAGCA+TGG | 0.491925 | 2:+80335196 | MsaT011003.1:CDS |
| AAGCAGCCAAAGAAAGGAAA+AGG | 0.495623 | 2:+80336045 | MsaT011003.1:CDS |
| ACTTCATCATGATTCACAAA+AGG | 0.507116 | 2:+80335411 | MsaT011003.1:CDS |
| ACTCATTCATCATCAACAAA+AGG | 0.509294 | 2:+80335624 | MsaT011003.1:CDS |
| AACTACCTTGTATTGAGAGA+GGG | 0.509993 | 2:-80335159 | None:intergenic |
| CCTATGAAGATTCGGAAAAG+AGG | 0.512041 | 2:+80334948 | MsaT011003.1:CDS |
| ACTTAATCATCGTTCACAAA+AGG | 0.517591 | 2:+80335129 | MsaT011003.1:CDS |
| CATAAAGAAGATCAAGATGA+TGG | 0.521149 | 2:+80335847 | MsaT011003.1:CDS |
| GCTTAAGGAAGTGAGATCTT+CGG | 0.523012 | 2:-80335384 | None:intergenic |
| CAAACCCCTCTCTCAATACA+AGG | 0.526964 | 2:+80335154 | MsaT011003.1:CDS |
| TGGGAATGGCAAGAATGAGC+TGG | 0.528852 | 2:+80335999 | MsaT011003.1:CDS |
| TCTTAAGAAAGTGAGATCTC+CGG | 0.549197 | 2:-80335102 | None:intergenic |
| TCTTAAGAAAGTGAGATCTC+CGG | 0.549197 | 2:-80335597 | None:intergenic |
| CCCGTGAAGAGTCTGAAAAG+AGG | 0.549764 | 2:+80335230 | MsaT011003.1:CDS |
| GAAAAGCATTGGGTGGTCAC+CGG | 0.556634 | 2:+80335083 | MsaT011003.1:CDS |
| GAAAAGCATTGGGTGGTCAC+CGG | 0.560535 | 2:+80335578 | MsaT011003.1:CDS |
| ACTACCTTGTATTGAGAAAG+GGG | 0.563148 | 2:-80335653 | None:intergenic |
| AAAACCCCTTTCTCAATACA+AGG | 0.568454 | 2:+80335649 | MsaT011003.1:CDS |
| CCATACAAAGCATTCATGGT+TGG | 0.569961 | 2:-80335766 | None:intergenic |
| CTATGAAGAGTCAGAAAAGA+GGG | 0.570873 | 2:+80335444 | MsaT011003.1:CDS |
| CCTATGAAGAGTCAGAAAAG+AGG | 0.571208 | 2:+80335443 | MsaT011003.1:CDS |
| ATCTTCTTTATGATATTCAG+TGG | 0.577502 | 2:-80335836 | None:intergenic |
| TCTTTATGATATTCAGTGGG+AGG | 0.578179 | 2:-80335832 | None:intergenic |
| TGGCAGAAGAGAGATAGAAG+AGG | 0.582371 | 2:+80335889 | MsaT011003.1:CDS |
| AAGCGATGAAGATGATGCTA+AGG | 0.582770 | 2:+80335492 | MsaT011003.1:CDS |
| GGGAATGGCAAGAATGAGCT+GGG | 0.589572 | 2:+80336000 | MsaT011003.1:CDS |
| TGAAGATGATGCTAAGGATG+AGG | 0.590398 | 2:+80335498 | MsaT011003.1:CDS |
| ACTACCTTGTATTGAGAGAG+GGG | 0.593179 | 2:-80335158 | None:intergenic |
| GTGTTGGTGAAACATCAAAT+GGG | 0.595282 | 2:+80335980 | MsaT011003.1:CDS |
| TAACTACCTTGTATTGAGAG+AGG | 0.601425 | 2:-80335160 | None:intergenic |
| TCTTCTTTATGATATTCAGT+GGG | 0.603725 | 2:-80335835 | None:intergenic |
| TAGTGATGAAGATGATGCTA+AGG | 0.613974 | 2:+80334997 | MsaT011003.1:CDS |
| AGCAATGGAAAAGCATTGGG+TGG | 0.615604 | 2:+80335076 | MsaT011003.1:CDS |
| AAGTGATGAAGATGATGCTA+AGG | 0.616457 | 2:+80335279 | MsaT011003.1:CDS |
| ATGGATAAAGATCATAAGCA+TGG | 0.623190 | 2:+80334914 | MsaT011003.1:CDS |
| ATGGTCACATGAGGACTCAC+CGG | 0.623316 | 2:+80335785 | MsaT011003.1:CDS |
| AGCAACGGAAAAGCATTGGG+TGG | 0.623901 | 2:+80335358 | MsaT011003.1:CDS |
| GGTGAAACATCAAATGGGAA+TGG | 0.636070 | 2:+80335985 | MsaT011003.1:CDS |
| TGAAGATGATGCTAAGGATG+AGG | 0.644459 | 2:+80335003 | MsaT011003.1:CDS |
| TGAAGATGATGCTAAGGATG+AGG | 0.644459 | 2:+80335285 | MsaT011003.1:CDS |
| AGTAACGGAAAAGCATTGGG+TGG | 0.649033 | 2:+80335571 | MsaT011003.1:CDS |
| GTGTATTGGTAGTGTTGAGG+CGG | 0.666307 | 2:+80335918 | MsaT011003.1:CDS |
| AAAAGTTCTTGTCATAACGA+AGG | 0.694153 | 2:-80335957 | None:intergenic |
| AAAGTGTATTGGTAGTGTTG+AGG | 0.694754 | 2:+80335915 | MsaT011003.1:CDS |
| ATGCTTTGTATGGTCACATG+AGG | 0.725028 | 2:+80335776 | MsaT011003.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 80334914 | 80336107 | 80334914 | ID=MsaG011003 |
| Chr2 | mRNA | 80334914 | 80336107 | 80334914 | ID=MsaT011003.1;Parent=MsaG011003 |
| Chr2 | exon | 80334914 | 80336107 | 80334914 | ID=MsaT011003.1.exon1;Parent=MsaT011003.1 |
| Chr2 | CDS | 80334914 | 80336107 | 80334914 | ID=cds.MsaT011003.1;Parent=MsaT011003.1 |
| Gene Sequence |
| Protein sequence |