AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011003


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011003 MtrunA17_Chr2g0331601 79.217 332 38 5 95 397 1 330 2.17e-175 492
MsaG011003 MtrunA17_Chr2g0331601 73.391 233 57 3 1 232 1 229 1.61e-101 304
MsaG011003 MtrunA17_Chr6g0481281 40.418 287 92 7 180 397 30 306 4.76e-53 178
MsaG011003 MtrunA17_Chr6g0481281 42.748 131 52 5 109 233 30 143 8.37e-18 83.6
MsaG011003 MtrunA17_Chr6g0481281 62.319 69 21 2 11 75 27 94 9.04e-17 80.5
MsaG011003 MtrunA17_Chr5g0448221 31.429 175 80 5 201 355 55 209 5.02e-14 71.6
MsaG011003 MtrunA17_Chr5g0425411 25.957 235 135 7 8 233 3 207 3.24e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011003 AT1G02030.1 27.132 258 95 8 41 232 1 231 6.55e-14 72.0
MsaG011003 AT5G56200.1 38.542 96 45 2 210 305 79 160 5.49e-13 70.9
MsaG011003 AT5G43170.1 29.114 158 80 2 80 233 12 141 5.90e-12 64.7
MsaG011003 AT2G17180.1 27.083 192 121 4 44 235 64 236 1.08e-11 65.5
MsaG011003 AT2G26940.1 37.143 105 61 1 210 309 49 153 1.24e-11 65.5
MsaG011003 AT3G19580.1 33.684 95 51 2 139 233 106 188 3.73e-11 63.9
MsaG011003 AT3G19580.2 33.684 95 51 2 139 233 106 188 3.73e-11 63.9
MsaG011003 AT3G60580.1 28.358 134 76 3 139 272 173 286 7.05e-11 63.2

Find 73 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCTCTCTTCTGCCATTT+TGG 0.136557 2:-80335883 None:intergenic
CAATACAAGGTAGTTATAAT+AGG 0.224337 2:+80335167 MsaT011003.1:CDS
CAATACAAGGTAGTACTAAT+AGG 0.256706 2:+80335662 MsaT011003.1:CDS
GTGATTTATCTTTGCCAAAA+TGG 0.304193 2:+80335869 MsaT011003.1:CDS
GTCCTTTAAAATCTCTATTC+CGG 0.308881 2:-80335804 None:intergenic
CATGATGAAGTTTCTGCTTA+AGG 0.329845 2:-80335399 None:intergenic
GTGACCATACAAAGCATTCA+TGG 0.407800 2:-80335770 None:intergenic
CCAACCATGAATGCTTTGTA+TGG 0.410676 2:+80335766 MsaT011003.1:CDS
AATACAAGGTAGTACTAATA+GGG 0.424373 2:+80335663 MsaT011003.1:CDS
TACTACCTTGTATTGAGAAA+GGG 0.428907 2:-80335654 None:intergenic
AATACAAGGTAGTTATAATA+GGG 0.431054 2:+80335168 MsaT011003.1:CDS
CACATCAGTGCAATGTTTGT+GGG 0.435235 2:+80335044 MsaT011003.1:CDS
ACACATCAGTGCAATGTTTG+TGG 0.442286 2:+80335538 MsaT011003.1:CDS
TTTAGCAATGGAAAAGCATT+GGG 0.444822 2:+80335073 MsaT011003.1:CDS
TCACATCAGTGCAATGTTTG+TGG 0.451639 2:+80335043 MsaT011003.1:CDS
GTACTACCTTGTATTGAGAA+AGG 0.452759 2:-80335655 None:intergenic
ACACATCAATGCAATGTTTG+TGG 0.453484 2:+80335325 MsaT011003.1:CDS
TTTAGTAACGGAAAAGCATT+GGG 0.453684 2:+80335568 MsaT011003.1:CDS
ATTCGGAAAAGAGGATGAAA+AGG 0.459648 2:+80334957 MsaT011003.1:CDS
TGAGAAAACCTATGAAGATT+CGG 0.461549 2:+80334940 MsaT011003.1:CDS
TTTAGCAACGGAAAAGCATT+GGG 0.462037 2:+80335355 MsaT011003.1:CDS
TGTGTTGGTGAAACATCAAA+TGG 0.466991 2:+80335979 MsaT011003.1:CDS
AGTCAGAAAAGAGGGTGAAA+AGG 0.473192 2:+80335452 MsaT011003.1:CDS
GAATGAGTCGTATGTTATAG+AGG 0.473597 2:+80336080 MsaT011003.1:CDS
GATGATGAAGAAGAAATTGA+TGG 0.474180 2:+80335703 MsaT011003.1:CDS
AGAAGAGGTAGAAAGTGTAT+TGG 0.483023 2:+80335904 MsaT011003.1:CDS
AGTCTGAAAAGAGGATAAAG+AGG 0.489348 2:+80335239 MsaT011003.1:CDS
AGTGATAAGCAGCCAAAGAA+AGG 0.491245 2:+80336039 MsaT011003.1:CDS
TTTGATAAAGATAGTAAGCA+TGG 0.491925 2:+80335196 MsaT011003.1:CDS
AAGCAGCCAAAGAAAGGAAA+AGG 0.495623 2:+80336045 MsaT011003.1:CDS
ACTTCATCATGATTCACAAA+AGG 0.507116 2:+80335411 MsaT011003.1:CDS
ACTCATTCATCATCAACAAA+AGG 0.509294 2:+80335624 MsaT011003.1:CDS
AACTACCTTGTATTGAGAGA+GGG 0.509993 2:-80335159 None:intergenic
CCTATGAAGATTCGGAAAAG+AGG 0.512041 2:+80334948 MsaT011003.1:CDS
ACTTAATCATCGTTCACAAA+AGG 0.517591 2:+80335129 MsaT011003.1:CDS
CATAAAGAAGATCAAGATGA+TGG 0.521149 2:+80335847 MsaT011003.1:CDS
GCTTAAGGAAGTGAGATCTT+CGG 0.523012 2:-80335384 None:intergenic
CAAACCCCTCTCTCAATACA+AGG 0.526964 2:+80335154 MsaT011003.1:CDS
TGGGAATGGCAAGAATGAGC+TGG 0.528852 2:+80335999 MsaT011003.1:CDS
TCTTAAGAAAGTGAGATCTC+CGG 0.549197 2:-80335102 None:intergenic
TCTTAAGAAAGTGAGATCTC+CGG 0.549197 2:-80335597 None:intergenic
CCCGTGAAGAGTCTGAAAAG+AGG 0.549764 2:+80335230 MsaT011003.1:CDS
GAAAAGCATTGGGTGGTCAC+CGG 0.556634 2:+80335083 MsaT011003.1:CDS
GAAAAGCATTGGGTGGTCAC+CGG 0.560535 2:+80335578 MsaT011003.1:CDS
ACTACCTTGTATTGAGAAAG+GGG 0.563148 2:-80335653 None:intergenic
AAAACCCCTTTCTCAATACA+AGG 0.568454 2:+80335649 MsaT011003.1:CDS
CCATACAAAGCATTCATGGT+TGG 0.569961 2:-80335766 None:intergenic
CTATGAAGAGTCAGAAAAGA+GGG 0.570873 2:+80335444 MsaT011003.1:CDS
CCTATGAAGAGTCAGAAAAG+AGG 0.571208 2:+80335443 MsaT011003.1:CDS
ATCTTCTTTATGATATTCAG+TGG 0.577502 2:-80335836 None:intergenic
TCTTTATGATATTCAGTGGG+AGG 0.578179 2:-80335832 None:intergenic
TGGCAGAAGAGAGATAGAAG+AGG 0.582371 2:+80335889 MsaT011003.1:CDS
AAGCGATGAAGATGATGCTA+AGG 0.582770 2:+80335492 MsaT011003.1:CDS
GGGAATGGCAAGAATGAGCT+GGG 0.589572 2:+80336000 MsaT011003.1:CDS
TGAAGATGATGCTAAGGATG+AGG 0.590398 2:+80335498 MsaT011003.1:CDS
ACTACCTTGTATTGAGAGAG+GGG 0.593179 2:-80335158 None:intergenic
GTGTTGGTGAAACATCAAAT+GGG 0.595282 2:+80335980 MsaT011003.1:CDS
TAACTACCTTGTATTGAGAG+AGG 0.601425 2:-80335160 None:intergenic
TCTTCTTTATGATATTCAGT+GGG 0.603725 2:-80335835 None:intergenic
TAGTGATGAAGATGATGCTA+AGG 0.613974 2:+80334997 MsaT011003.1:CDS
AGCAATGGAAAAGCATTGGG+TGG 0.615604 2:+80335076 MsaT011003.1:CDS
AAGTGATGAAGATGATGCTA+AGG 0.616457 2:+80335279 MsaT011003.1:CDS
ATGGATAAAGATCATAAGCA+TGG 0.623190 2:+80334914 MsaT011003.1:CDS
ATGGTCACATGAGGACTCAC+CGG 0.623316 2:+80335785 MsaT011003.1:CDS
AGCAACGGAAAAGCATTGGG+TGG 0.623901 2:+80335358 MsaT011003.1:CDS
GGTGAAACATCAAATGGGAA+TGG 0.636070 2:+80335985 MsaT011003.1:CDS
TGAAGATGATGCTAAGGATG+AGG 0.644459 2:+80335003 MsaT011003.1:CDS
TGAAGATGATGCTAAGGATG+AGG 0.644459 2:+80335285 MsaT011003.1:CDS
AGTAACGGAAAAGCATTGGG+TGG 0.649033 2:+80335571 MsaT011003.1:CDS
GTGTATTGGTAGTGTTGAGG+CGG 0.666307 2:+80335918 MsaT011003.1:CDS
AAAAGTTCTTGTCATAACGA+AGG 0.694153 2:-80335957 None:intergenic
AAAGTGTATTGGTAGTGTTG+AGG 0.694754 2:+80335915 MsaT011003.1:CDS
ATGCTTTGTATGGTCACATG+AGG 0.725028 2:+80335776 MsaT011003.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 80334914 80336107 80334914 ID=MsaG011003
Chr2 mRNA 80334914 80336107 80334914 ID=MsaT011003.1;Parent=MsaG011003
Chr2 exon 80334914 80336107 80334914 ID=MsaT011003.1.exon1;Parent=MsaT011003.1
Chr2 CDS 80334914 80336107 80334914 ID=cds.MsaT011003.1;Parent=MsaT011003.1
Gene Sequence

>MsaT011003.1

ATGGATAAAGATCATAAGCATGGTTATGAGAAAACCTATGAAGATTCGGAAAAGAGGATGAAAAGGTCTTCATGTTCACTATCTAGTGATGAAGATGATGCTAAGGATGAGGTTTCTGTTCAAGAATCTTCACATCAGTGCAATGTTTGTGGGAAAACTTTTAGCAATGGAAAAGCATTGGGTGGTCACCGGAGATCTCACTTTCTTAAGAAGAAACTTAATCATCGTTCACAAAAGGTACAAACCCCTCTCTCAATACAAGGTAGTTATAATAGGGCTAGTTTTGATAAAGATAGTAAGCATGGTTTTGAGAACACCCGTGAAGAGTCTGAAAAGAGGATAAAGAGGTCTTTTAGTTCTCTATCAAGTGATGAAGATGATGCTAAGGATGAGGTTTCTATTCAAGAATCTACACATCAATGCAATGTTTGTGGAAAAACTTTTAGCAACGGAAAAGCATTGGGTGGTCACCGAAGATCTCACTTCCTTAAGCAGAAACTTCATCATGATTCACAAAAGGTTGATAATACCTATGAAGAGTCAGAAAAGAGGGTGAAAAGGTCAGCTTGTTCTCTATCAAGCGATGAAGATGATGCTAAGGATGAGGTTTCTGTTCAAGAATCTACACATCAGTGCAATGTTTGTGGAAAAACTTTTAGTAACGGAAAAGCATTGGGTGGTCACCGGAGATCTCACTTTCTTAAGAAGAAACTCATTCATCATCAACAAAAGGTGAAAACCCCTTTCTCAATACAAGGTAGTACTAATAGGGCTAGTTTTGATGATTATGATGATGAAGAAGAAATTGATGGCATAGAGAAACCAACTTGTTCTCTCTGCAAAAAGAAGTTTCCAACCATGAATGCTTTGTATGGTCACATGAGGACTCACCGGAATAGAGATTTTAAAGGACTTAATCCTCCCACTGAATATCATAAAGAAGATCAAGATGATGGTGATTTATCTTTGCCAAAATGGCAGAAGAGAGATAGAAGAGGTAGAAAGTGTATTGGTAGTGTTGAGGCGGCTGCAAATCTTTTGCACCTTCGTTATGACAAGAACTTTTGTGTTGGTGAAACATCAAATGGGAATGGCAAGAATGAGCTGGGCAGTGATAGAAACAGCAGTGATAAGCAGCCAAAGAAAGGAAAAGGTTTATTTGATTTGAATGAGTCGTATGTTATAGAGGACTAA

Protein sequence

>MsaT011003.1

MDKDHKHGYEKTYEDSEKRMKRSSCSLSSDEDDAKDEVSVQESSHQCNVCGKTFSNGKALGGHRRSHFLKKKLNHRSQKVQTPLSIQGSYNRASFDKDSKHGFENTREESEKRIKRSFSSLSSDEDDAKDEVSIQESTHQCNVCGKTFSNGKALGGHRRSHFLKQKLHHDSQKVDNTYEESEKRVKRSACSLSSDEDDAKDEVSVQESTHQCNVCGKTFSNGKALGGHRRSHFLKKKLIHHQQKVKTPFSIQGSTNRASFDDYDDEEEIDGIEKPTCSLCKKKFPTMNALYGHMRTHRNRDFKGLNPPTEYHKEDQDDGDLSLPKWQKRDRRGRKCIGSVEAAANLLHLRYDKNFCVGETSNGNGKNELGSDRNSSDKQPKKGKGLFDLNESYVIED*