AlfalfaGEDB | MsaG011039

M. sativa ssp. caerulea / MsaG011039

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011039	XP_013465529.1	97.426	272	6	1	1	271	225	496	0.0	545

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011039	sp\|Q9LJG8\|ASIL2_ARATH	42.453	106	54	3	50	152	84	185	2.45e-22	99.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011039	tr\|A0A072VC01\|A0A072VC01_MEDTR	97.426	272	6	1	1	271	225	496	0.0	545

TFs/TRs:

Gene ID	Type	Classification
MsaG011039	TF	Trihelix

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000080	MsaG011039	0.826455	4.784906e-54	1.253242e-51
MsaG000309	MsaG011039	0.871916	9.716670e-67	1.221836e-63
MsaG000382	MsaG011039	0.817691	5.035071e-52	1.042568e-49
MsaG001087	MsaG011039	0.809846	2.642138e-50	4.495354e-48
MsaG002458	MsaG011039	0.829913	7.089100e-55	2.046535e-52
MsaG002956	MsaG011039	0.845017	9.893415e-59	4.536514e-56
MsaG002961	MsaG011039	0.841278	9.709596e-58	3.946116e-55
MsaG002973	MsaG011039	0.811817	9.940713e-51	1.774749e-48
MsaG003102	MsaG011039	0.807284	9.254711e-50	1.480437e-47
MsaG003679	MsaG011039	0.850792	2.575611e-60	1.434552e-57
MsaG003998	MsaG011039	0.808411	5.344929e-50	8.784335e-48
MsaG004012	MsaG011039	0.829840	7.381210e-55	2.126418e-52
MsaG004041	MsaG011039	0.802664	8.469207e-49	1.216460e-46
MsaG004225	MsaG011039	0.832077	2.098055e-55	6.447811e-53
MsaG004414	MsaG011039	0.802824	7.850859e-49	1.131737e-46
MsaG005018	MsaG011039	0.820990	8.990952e-53	2.030005e-50
MsaG005334	MsaG011039	0.805552	2.137392e-49	3.282129e-47
MsaG006037	MsaG011039	0.861187	2.413308e-63	1.963062e-60
MsaG006330	MsaG011039	0.815608	1.468066e-51	2.881966e-49
MsaG006886	MsaG011039	0.805522	2.168434e-49	3.327509e-47
MsaG006997	MsaG011039	0.808775	4.471986e-50	7.414729e-48
MsaG007459	MsaG011039	0.801654	1.363229e-48	1.913380e-46
MsaG008425	MsaG011039	0.812511	7.026499e-51	1.276189e-48
MsaG008881	MsaG011039	0.804024	4.440510e-49	6.581738e-47
MsaG009050	MsaG011039	0.867937	1.909949e-65	2.031968e-62
MsaG010592	MsaG011039	0.859877	5.993072e-63	4.637546e-60
MsaG011039	MsaG011153	0.802833	7.818669e-49	1.127323e-46
MsaG011039	MsaG011348	0.813122	5.170942e-51	9.535501e-49
MsaG011039	MsaG011772	0.813855	3.573622e-51	6.712351e-49
MsaG011039	MsaG011754	0.823095	2.939455e-53	7.020931e-51
MsaG011039	MsaG012226	0.806857	1.138284e-49	1.802583e-47
MsaG011039	MsaG013962	0.815900	1.264311e-51	2.500391e-49
MsaG011039	MsaG014468	0.851083	2.134156e-60	1.200668e-57
MsaG011039	MsaG014676	0.839976	2.120860e-57	8.273042e-55
MsaG011039	MsaG014719	0.804176	4.130526e-49	6.143662e-47
MsaG011039	MsaG015081	0.827559	2.612078e-54	7.055515e-52
MsaG011039	MsaG015143	0.823089	2.948687e-53	7.042020e-51
MsaG011039	MsaG015689	0.822345	4.384847e-53	1.026365e-50
MsaG011039	MsaG016078	0.800507	2.333477e-48	3.191308e-46
MsaG011039	MsaG016571	0.815386	1.644034e-51	3.209257e-49
MsaG011039	MsaG016674	0.819566	1.899970e-52	4.130794e-50
MsaG011039	MsaG016882	0.832022	2.164608e-55	6.641690e-53
MsaG011039	MsaG017269	0.850410	3.294098e-60	1.810471e-57
MsaG011039	MsaG017470	0.853278	5.105523e-61	3.102038e-58
MsaG011039	MsaG018111	0.829213	1.046712e-54	2.961519e-52
MsaG011039	MsaG018123	0.838149	6.274940e-57	2.311974e-54
MsaG011039	MsaG018270	0.859658	6.969662e-63	5.348757e-60
MsaG011039	MsaG018322	0.811442	1.198154e-50	2.119486e-48
MsaG011039	MsaG018821	0.804925	2.887644e-49	4.370226e-47
MsaG011039	MsaG019542	0.835407	3.115336e-56	1.056311e-53
MsaG011039	MsaG019594	0.803991	4.511282e-49	6.681255e-47
MsaG011039	MsaG020029	0.801571	1.417555e-48	1.985863e-46
MsaG011039	MsaG020338	0.808186	5.965486e-50	9.751497e-48
MsaG011039	MsaG020487	0.877275	1.495230e-68	2.380641e-65
MsaG011039	MsaG020488	0.838037	6.703196e-57	2.461238e-54
MsaG011039	MsaG021286	0.823739	2.082349e-53	5.061415e-51
MsaG011039	MsaG021993	0.802804	7.926303e-49	1.142103e-46
MsaG011039	MsaG022506	0.802954	7.384083e-49	1.067648e-46
MsaG011039	MsaG022595	0.808344	5.521319e-50	9.059773e-48
MsaG011039	MsaG022970	0.808690	4.662805e-50	7.715450e-48
MsaG011039	MsaG023205	0.832244	1.908696e-55	5.893822e-53
MsaG011039	MsaG023761	0.821923	5.487835e-53	1.270065e-50
MsaG011039	MsaG023762	0.831081	3.682941e-55	1.099375e-52
MsaG011039	MsaG024387	0.853200	5.373638e-61	3.255949e-58
MsaG011039	MsaG027650	0.859024	1.077622e-62	8.074210e-60
MsaG011039	MsaG027933	0.814532	2.536975e-51	4.846624e-49
MsaG011039	MsaG028633	0.805227	2.497865e-49	3.806971e-47
MsaG011039	MsaG028758	0.858835	1.227333e-62	9.130043e-60
MsaG011039	MsaG028759	0.839532	2.763400e-57	1.063001e-54
MsaG011039	MsaG028804	0.837443	9.503957e-57	3.426863e-54
MsaG011039	MsaG029117	0.826225	5.424646e-54	1.411621e-51
MsaG011039	MsaG029483	0.816750	8.178053e-52	1.652864e-49
MsaG011039	MsaG030158	0.830112	6.340223e-55	1.840840e-52
MsaG011039	MsaG031374	0.805782	1.913557e-49	2.954356e-47
MsaG011039	MsaG031652	0.802876	7.659979e-49	1.105554e-46
MsaG011039	MsaG032101	0.822883	3.292990e-53	7.820481e-51
MsaG011039	MsaG033400	0.818684	3.008738e-52	6.392043e-50
MsaG011039	MsaG033596	0.838159	6.235836e-57	2.298306e-54
MsaG011039	MsaG034418	0.826184	5.546916e-54	1.441826e-51
MsaG011039	MsaG035012	0.812206	8.184405e-51	1.475199e-48
MsaG011039	MsaG035054	0.844230	1.608346e-58	7.185595e-56
MsaG011039	MsaG036303	0.835885	2.360985e-56	8.120868e-54
MsaG011039	MsaG036814	0.803509	5.673687e-49	8.309424e-47
MsaG011039	MsaG037009	0.814791	2.225571e-51	4.279590e-49
MsaG011039	MsaG037910	0.810357	2.052510e-50	3.535861e-48
MsaG011039	MsaG038015	0.800907	1.935587e-48	2.671096e-46
MsaG011039	MsaG040026	0.821546	6.702024e-53	1.535571e-50
MsaG011039	MsaG040381	0.819060	2.474312e-52	5.308903e-50
MsaG011039	MsaG040649	0.855629	1.074369e-61	7.101166e-59
MsaG011039	MsaG041335	0.813314	4.695757e-51	8.700626e-49
MsaG011039	MsaG042378	0.813864	3.557520e-51	6.683543e-49
MsaG011039	MsaG043227	0.828768	1.340361e-54	3.745045e-52
MsaG011039	MsaG044002	0.831685	2.620001e-55	7.960372e-53
MsaG011039	MsaG044028	0.820373	1.244221e-52	2.763725e-50
MsaG011039	MsaG044333	0.824608	1.304433e-53	3.246259e-51
MsaG011039	MsaG044501	0.808418	5.324938e-50	8.753144e-48
MsaG011039	MsaG044885	0.847731	1.815000e-59	9.109085e-57
MsaG011039	MsaG045078	0.806414	1.410932e-49	2.211222e-47
MsaG011039	MsaG045576	0.821153	8.252211e-53	1.871244e-50
MsaG011039	MsaG045809	0.800380	2.475898e-48	3.376449e-46
MsaG011039	MsaG047152	0.832506	1.644843e-55	5.118050e-53
MsaG011039	MsaG000837	0.851596	1.531476e-60	8.769822e-58
MsaG011039	MsaG002745	0.812939	5.668069e-51	1.040465e-48
MsaG011039	MsaG007366	0.904756	2.070633e-79	1.405422e-75
MsaG011039	MsaG009207	0.844485	1.374161e-58	6.190560e-56
MsaG011039	MsaG014863	0.833859	7.602617e-56	2.461163e-53
MsaG011039	MsaG015244	0.841648	7.765073e-58	3.193013e-55
MsaG011039	MsaG013378	0.828627	1.449362e-54	4.033828e-52
MsaG011039	MsaG011654	0.857872	2.367970e-62	1.699478e-59
MsaG011039	MsaG011633	0.807470	8.455507e-50	1.358618e-47
MsaG011039	MsaG013857	0.817025	7.099149e-52	1.445064e-49
MsaG011039	MsaG013372	0.827738	2.367435e-54	6.426750e-52
MsaG011039	MsaG015300	0.815428	1.609093e-51	3.144386e-49
MsaG011039	MsaG011352	0.838500	5.097472e-57	1.898812e-54
MsaG011039	MsaG020426	0.806205	1.560513e-49	2.433619e-47
MsaG011039	MsaG023458	0.814708	2.320624e-51	4.452975e-49
MsaG011039	MsaG027627	0.801506	1.461534e-48	2.044456e-46
MsaG011039	MsaG028127	0.807313	9.123762e-50	1.460509e-47
MsaG011039	MsaG031811	0.842203	5.549712e-58	2.322713e-55
MsaG011039	MsaG032342	0.846806	3.247721e-59	1.580022e-56
MsaG011039	MsaG033234	0.825051	1.026525e-53	2.585871e-51
MsaG011039	MsaG031391	0.806261	1.518956e-49	2.371933e-47
MsaG011039	MsaG033676	0.806962	1.081946e-49	1.717601e-47
MsaG011039	MsaG036784	0.815963	1.224256e-51	2.425089e-49
MsaG011039	MsaG038711	0.825064	1.019451e-53	2.568986e-51
MsaG011039	MsaG036963	0.804845	3.000612e-49	4.532819e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011039	MtrunA17_Chr2g0331931	97.426	272	6	1	1	271	225	496	0.0	545
MsaG011039	MtrunA17_Chr6g0485241	52.252	222	101	3	49	267	129	348	2.36e-62	200
MsaG011039	MtrunA17_Chr8g0344681	28.758	306	141	10	29	266	37	333	5.59e-21	91.3
MsaG011039	MtrunA17_Chr4g0041151	43.158	95	48	2	50	138	31	125	5.82e-18	82.0
MsaG011039	MtrunA17_Chr4g0041151	40.541	74	44	0	195	268	220	293	5.26e-11	62.0
MsaG011039	MtrunA17_Chr2g0283871	39.362	94	52	2	50	138	23	116	3.95e-17	79.7
MsaG011039	MtrunA17_Chr2g0325841	31.250	112	65	2	30	137	34	137	6.77e-12	65.5
MsaG011039	MtrunA17_Chr1g0164461	25.850	147	100	3	49	191	37	178	1.79e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011039	AT3G24490.1	42.912	261	129	4	12	271	65	306	1.14e-61	197
MsaG011039	AT3G14180.1	42.453	106	54	3	50	152	84	185	2.49e-23	99.0
MsaG011039	AT3G11100.1	33.182	220	137	3	50	265	22	235	6.86e-21	89.4
MsaG011039	AT1G54060.1	31.439	264	137	8	50	271	94	355	1.29e-18	85.1
MsaG011039	AT5G05550.3	28.814	236	129	5	50	269	24	236	1.97e-17	79.7
MsaG011039	AT5G05550.1	28.814	236	129	5	50	269	24	236	2.38e-17	79.7
MsaG011039	AT5G05550.2	28.814	236	129	5	50	269	24	236	2.79e-17	79.3
MsaG011039	AT3G58630.2	37.255	102	50	2	50	137	26	127	2.90e-16	77.4
MsaG011039	AT3G58630.1	37.255	102	50	2	50	137	26	127	3.21e-16	77.4
MsaG011039	AT3G10030.2	34.783	92	59	1	49	139	160	251	5.10e-14	72.0
MsaG011039	AT3G10030.1	34.783	92	59	1	49	139	160	251	5.99e-14	72.0
MsaG011039	AT3G24860.1	30.400	125	77	3	35	153	50	170	8.55e-13	67.8

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGATTCCATTCAGAATTT+TGG	0.118439	2:-80624851	MsaT011039.1:CDS
CAAGATTAAGTTTCAAGATA+TGG	0.238032	2:-80625149	MsaT011039.1:CDS
GCAGCTGCTAAACGATCTTT+TGG	0.284124	2:-80625385	MsaT011039.1:CDS
TGGGTGGTGGAGATAGAAAA+TGG	0.290828	2:-80625129	MsaT011039.1:CDS
CATTTGTTCTTCTTGATGCT+TGG	0.296454	2:-80625324	MsaT011039.1:CDS
ATTTGTTCTTCTTGATGCTT+GGG	0.319429	2:-80625323	MsaT011039.1:CDS
AGAAGATTGAAAATAATAAA+AGG	0.319723	2:-80624817	MsaT011039.1:CDS
ACAGAATCGGTTCGTGTAGA+AGG	0.331232	2:-80624947	MsaT011039.1:CDS
GATGGATTTCCTCAACGATT+TGG	0.369450	2:-80624765	MsaT011039.1:CDS
TTCTTGATGCTTGGGGTGAT+CGG	0.409344	2:-80625315	MsaT011039.1:CDS
ATCGGTTTCTGCAACATAGA+AGG	0.423435	2:-80625297	MsaT011039.1:CDS
AAGATTAAGTTTCAAGATAT+GGG	0.423794	2:-80625148	MsaT011039.1:CDS
AACTTATGAATTTGCAGCTC+GGG	0.427130	2:-80625425	MsaT011039.1:CDS
ATGGCAACAAGTTGCAGAAA+AGG	0.453580	2:-80625254	MsaT011039.1:CDS
TAATAAAAGGCAGCAGATGA+TGG	0.461717	2:-80624804	MsaT011039.1:CDS
GTTTCAAAAGTTGAAAGAAC+GGG	0.463936	2:-80625223	MsaT011039.1:CDS
GGGTGGTGGAGATAGAAAAT+GGG	0.465027	2:-80625128	MsaT011039.1:CDS
CCTTCTCAGTCCATTCAGTA+AGG	0.479775	2:+80625350	None:intergenic
GCAGCTGCAGCTGACGGTGT+TGG	0.482446	2:+80625400	None:intergenic
TTAGGAATTCCCTTACTGAA+TGG	0.483595	2:-80625360	MsaT011039.1:CDS
GGAATCAGCAAGTAATTTGA+AGG	0.488399	2:+80624865	None:intergenic
TAATTCATCTTCATTCATCA+TGG	0.493258	2:+80625489	None:intergenic
TATCTTACCAAAATTCTGAA+TGG	0.497261	2:+80624844	None:intergenic
TCCTCCTGAAAATACAGAAT+CGG	0.498451	2:-80624960	MsaT011039.1:CDS
GCATGATTTACATAAACTCC+AGG	0.504047	2:+80625010	None:intergenic
CAAAGAAAAGAAGGAAAGGA+AGG	0.514012	2:-80624907	MsaT011039.1:CDS
GGCCTTGCCCAAAGAAAAGA+AGG	0.520654	2:-80624916	MsaT011039.1:CDS
AAGTTTCAAGATATGGGTGG+TGG	0.524195	2:-80625142	MsaT011039.1:CDS
TCTCTTGAGTTCGAACGACA+AGG	0.531751	2:+80625049	None:intergenic
GGAACTAGAAAAGATGAGGA+TGG	0.544427	2:-80624783	MsaT011039.1:CDS
GGGAGTCTTGCTTGTTGTGG+TGG	0.550724	2:+80625070	None:intergenic
AAAGAAAAGAAGGAAAGGAA+GGG	0.551393	2:-80624906	MsaT011039.1:CDS
TTTGTTCTTCTTGATGCTTG+GGG	0.554775	2:-80625322	MsaT011039.1:CDS
AAGAAAAGAAGGAAAGGAAG+GGG	0.556042	2:-80624905	MsaT011039.1:CDS
GTTCGTGTAGAAGGTTTCGA+AGG	0.557020	2:-80624938	MsaT011039.1:CDS
CGTTTAGCAGCTGCAGCTGA+CGG	0.562335	2:+80625394	None:intergenic
CTCTTGAGTTCGAACGACAA+GGG	0.566224	2:+80625050	None:intergenic
GGTTTCAAAAGTTGAAAGAA+CGG	0.571460	2:-80625224	MsaT011039.1:CDS
TGTAAATCATGCAAATGAGA+TGG	0.574988	2:-80624999	MsaT011039.1:CDS
AAGGAAGAGTCTTCGATCTG+AGG	0.584495	2:-80625278	MsaT011039.1:CDS
TGCCCAAAGAAAAGAAGGAA+AGG	0.586775	2:-80624911	MsaT011039.1:CDS
CAACTTATGAATTTGCAGCT+CGG	0.587886	2:-80625426	MsaT011039.1:CDS
CAAGGGAGTCTTGCTTGTTG+TGG	0.589582	2:+80625067	None:intergenic
CTTCTCAGTCCATTCAGTAA+GGG	0.589672	2:+80625351	None:intergenic
AGAGTCTTCGATCTGAGGAA+TGG	0.593143	2:-80625273	MsaT011039.1:CDS
AGTTGCAGAAAAGGTATCAG+TGG	0.596635	2:-80625245	MsaT011039.1:CDS
TTCGTGTAGAAGGTTTCGAA+GGG	0.597829	2:-80624937	MsaT011039.1:CDS
CCTTACTGAATGGACTGAGA+AGG	0.603468	2:-80625350	MsaT011039.1:CDS
TGATGGAACTAGAAAAGATG+AGG	0.616160	2:-80624787	MsaT011039.1:CDS
AAATGAGATGGATGACATGA+AGG	0.616691	2:-80624987	MsaT011039.1:CDS
ATTAAGTTTCAAGATATGGG+TGG	0.645987	2:-80625145	MsaT011039.1:CDS
TTTGTGCTTCCAAATCGTTG+AGG	0.661381	2:+80624756	None:intergenic
TGAATGAAGATGAATTAGAG+TGG	0.724734	2:-80625483	MsaT011039.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	80624694	80625509	80624694	ID=MsaG011039
Chr2	mRNA	80624694	80625509	80624694	ID=MsaT011039.1;Parent=MsaG011039
Chr2	exon	80624694	80625509	80624694	ID=MsaT011039.1.exon1;Parent=MsaT011039.1
Chr2	CDS	80624694	80625509	80624694	ID=cds.MsaT011039.1;Parent=MsaT011039.1

Gene Sequence

>MsaT011039.1

ATGATGAATGAAGATGAATTAGAGTGGCACCCAAAAAAGCAAAAGCTAAAGAGTTTGATGTCAACTTATGAATTTGCAGCTCGGGTGCCAACACCGTCAGCTGCAGCTGCTAAACGATCTTTTGGGGTTAGGAATTCCCTTACTGAATGGACTGAGAAGGAGACATTTGTTCTTCTTGATGCTTGGGGTGATCGGTTTCTGCAACATAGAAGGAAGAGTCTTCGATCTGAGGAATGGCAACAAGTTGCAGAAAAGGTATCAGTGGTTTCAAAAGTTGAAAGAACGGGTACTCAGTGTAGAAATCGTTTAGATACATTGAAGAAAAAATACAAAAAGGACAAGATTAAGTTTCAAGATATGGGTGGTGGAGATAGAAAATGGGTTTATTTTCAAAAGATGGATAAACTAATGTCTTCTCCACCACAACAAGCAAGACTCCCTTGTCGTTCGAACTCAAGAGAGCGTATTTTTATGAAGCCTGGAGTTTATGTAAATCATGCAAATGAGATGGATGACATGAAGGATAGTCCTCCTGAAAATACAGAATCGGTTCGTGTAGAAGGTTTCGAAGGGCCTTGCCCAAAGAAAAGAAGGAAAGGAAGGGGAAGTGATGAGTTTTCTTCCTTCAAATTACTTGCTGATTCCATTCAGAATTTTGGTAAGATATATGAGAAGATTGAAAATAATAAAAGGCAGCAGATGATGGAACTAGAAAAGATGAGGATGGATTTCCTCAACGATTTGGAAGCACAAAAGAGACAGATTTTTGAAAGATTGCAGAGTGAGATTTTAAAAATCGAGCAAAGAGATGATTAA

Protein sequence

>MsaT011039.1

MMNEDELEWHPKKQKLKSLMSTYEFAARVPTPSAAAAKRSFGVRNSLTEWTEKETFVLLDAWGDRFLQHRRKSLRSEEWQQVAEKVSVVSKVERTGTQCRNRLDTLKKKYKKDKIKFQDMGGGDRKWVYFQKMDKLMSSPPQQARLPCRSNSRERIFMKPGVYVNHANEMDDMKDSPPENTESVRVEGFEGPCPKKRRKGRGSDEFSSFKLLADSIQNFGKIYEKIENNKRQQMMELEKMRMDFLNDLEAQKRQIFERLQSEILKIEQRDD*