Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011091 | XP_003597853.1 | 88.950 | 181 | 18 | 2 | 1 | 179 | 1 | 181 | 2.22e-106 | 312 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011091 | tr|G7IUS5|G7IUS5_MEDTR | 88.950 | 181 | 18 | 2 | 1 | 179 | 1 | 181 | 1.06e-106 | 312 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000140 | MsaG011091 | 0.801495 | 1.469448e-48 | 2.054979e-46 |
| MsaG000153 | MsaG011091 | 0.820029 | 1.490328e-52 | 3.280401e-50 |
| MsaG000344 | MsaG011091 | 0.837420 | 9.637180e-57 | 3.472350e-54 |
| MsaG000400 | MsaG011091 | 0.815214 | 1.794611e-51 | 3.487996e-49 |
| MsaG000648 | MsaG011091 | 0.829725 | 7.871524e-55 | 2.260216e-52 |
| MsaG000684 | MsaG011091 | 0.828802 | 1.315402e-54 | 3.678850e-52 |
| MsaG000778 | MsaG011091 | 0.801683 | 1.345162e-48 | 1.889264e-46 |
| MsaG000968 | MsaG011091 | 0.820975 | 9.064439e-53 | 2.045746e-50 |
| MsaG001037 | MsaG011091 | 0.800911 | 1.932117e-48 | 2.666532e-46 |
| MsaG001038 | MsaG011091 | 0.800078 | 2.850725e-48 | 3.861122e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011091 | MtrunA17_Chr2g0332631 | 86.869 | 99 | 11 | 2 | 1 | 97 | 1 | 99 | 1.42e-51 | 162 |
Find 30 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTCACAAACCCATATG+AGG | 0.470113 | 2:+81285860 | MsaT011091.1:CDS |
| AAATACCTCAGCCATCACAG+CGG | 0.800744 | 2:+81287372 | MsaT011091.1:CDS |
| AACGACCTCAGAACAGCAAC+AGG | 0.451715 | 2:+81285964 | MsaT011091.1:CDS |
| ACATAATGAAGAGGTAGAGG+AGG | 0.543147 | 2:-81286063 | None:intergenic |
| ACTACCTCTTTACATAAGAA+TGG | 0.478765 | 2:+81287397 | MsaT011091.1:CDS |
| AGAGGTAGTTGCCGCTGTGA+TGG | 0.562973 | 2:-81287383 | None:intergenic |
| AGCCATGTGCATAGTTGCAT+TGG | 0.488645 | 2:-81285939 | None:intergenic |
| AGTTGCCGCTGTGATGGCTG+AGG | 0.641938 | 2:-81287377 | None:intergenic |
| AGTTTGTGAATGCAGAACCA+GGG | 0.757579 | 2:+81287230 | MsaT011091.1:intron |
| ATGAACATTCTACAGAATCT+TGG | 0.480970 | 2:-81285988 | None:intergenic |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 81285844 | 81287495 | 81285844 | ID=MsaG011091 |
| Chr2 | mRNA | 81285844 | 81287495 | 81285844 | ID=MsaT011091.1;Parent=MsaG011091 |
| Chr2 | exon | 81285844 | 81286132 | 81285844 | ID=MsaT011091.1.exon1;Parent=MsaT011091.1 |
| Chr2 | CDS | 81285844 | 81286132 | 81285844 | ID=cds.MsaT011091.1;Parent=MsaT011091.1 |
| Chr2 | exon | 81287245 | 81287495 | 81287245 | ID=MsaT011091.1.exon2;Parent=MsaT011091.1 |
| Chr2 | CDS | 81287245 | 81287495 | 81287245 | ID=cds.MsaT011091.1;Parent=MsaT011091.1 |
| Gene Sequence |
>MsaT011091.1 ATGGATTCAGAAGCTGAAAATTCACAAACCCATATGAGGATTAAGACTCAGCTATGCAAAAGATTCATGCAAGGAACGTGTCCTCTTCTTGCACCCCAATGCAACTATGCACATGGCTACAACGACCTCAGAACAGCAACAGGACCAAGATTCTGTAGAATGTTCATGCACACCAGACATTGTAGCTACGGACACAACTGTCGTTTCCTTCATGCAACTCCTCCTCTACCTCTTCATTATGTTCATCCTCAGCAGCAGCAGCAGCAGGAGAACTTTTCTCCACAACCACAACCAGGGATGATACAAAATGCAGAATGCAGGATCATGCAAACTTCTGCTGCTGCTACAACTAATGGCCACTCTTCTGCACAAAACAACACAACGCAATTGCAAACTTATAGCTCTACTCCTGCTACAAATACCTCAGCCATCACAGCGGCAACTACCTCTTTACATAAGAATGGACCGTATGCTGGTAAATCCCTATCTGACAAAAACACAATGCAAAGGGTCATTCGTCTCTATGCTGATTGGATTTAA |
| Protein sequence |
>MsaT011091.1 MDSEAENSQTHMRIKTQLCKRFMQGTCPLLAPQCNYAHGYNDLRTATGPRFCRMFMHTRHCSYGHNCRFLHATPPLPLHYVHPQQQQQQENFSPQPQPGMIQNAECRIMQTSAAATTNGHSSAQNNTTQLQTYSSTPATNTSAITAATTSLHKNGPYAGKSLSDKNTMQRVIRLYADWI* |
