AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011213

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011213 MtrunA17_Chr2g0333851 95.327 321 6 5 1 317 1 316 0.0 613
MsaG011213 MtrunA17_Chr4g0001361 59.816 326 106 8 5 317 4 317 2.61e-107 315
MsaG011213 MtrunA17_Chr1g0150281 46.643 283 127 7 41 314 9 276 4.72e-71 221
MsaG011213 MtrunA17_Chr3g0136961 46.545 275 114 7 56 314 29 286 2.53e-70 220
MsaG011213 MtrunA17_Chr1g0150271 35.897 273 129 8 51 316 6 239 9.26e-40 140
MsaG011213 MtrunA17_Chr7g0270831 27.848 316 149 9 57 310 100 398 1.41e-29 117
MsaG011213 MtrunA17_Chr4g0075201 30.357 224 113 5 49 236 116 332 1.05e-27 112
MsaG011213 MtrunA17_Chr2g0288871 32.911 237 95 8 49 231 139 365 3.65e-26 108
MsaG011213 MtrunA17_Chr2g0302391 31.579 266 123 8 55 275 70 321 3.37e-24 102
MsaG011213 MtrunA17_Chr3g0099641 30.870 230 110 6 47 231 86 311 3.45e-24 102
MsaG011213 MtrunA17_Chr4g0051181 53.086 81 33 2 151 231 228 303 1.17e-23 100
MsaG011213 MtrunA17_Chr7g0216891 47.619 84 42 1 146 229 196 277 1.20e-22 98.2
MsaG011213 MtrunA17_Chr4g0060271 51.250 80 37 1 152 231 238 315 1.49e-22 98.2
MsaG011213 MtrunA17_Chr7g0270191 50.617 81 37 2 151 231 127 204 3.09e-22 94.4
MsaG011213 MtrunA17_Chr1g0194481 54.545 77 32 2 156 232 145 218 3.42e-22 94.7
MsaG011213 MtrunA17_Chr2g0294231 53.086 81 35 2 156 236 190 267 9.24e-22 94.4
MsaG011213 MtrunA17_Chr4g0051881 51.948 77 34 2 156 232 165 238 1.81e-21 93.2
MsaG011213 MtrunA17_Chr4g0025051 50.000 78 37 1 155 232 321 396 3.29e-21 94.0
MsaG011213 MtrunA17_Chr2g0322391 31.174 247 88 9 57 231 173 409 1.60e-20 92.0
MsaG011213 MtrunA17_Chr1g0188941 41.667 96 40 2 137 232 32 111 1.83e-20 85.1
MsaG011213 MtrunA17_Chr5g0445311 57.812 64 26 1 169 232 362 424 4.61e-20 90.5
MsaG011213 MtrunA17_Chr5g0445311 46.269 67 31 3 172 238 201 262 1.22e-11 65.5
MsaG011213 MtrunA17_Chr4g0025061 50.000 78 37 1 155 232 346 421 5.02e-20 90.5
MsaG011213 MtrunA17_Chr4g0004911 40.909 110 49 2 122 231 302 395 5.87e-20 90.1
MsaG011213 MtrunA17_Chr4g0004911 49.231 65 32 1 171 235 184 247 3.62e-16 79.0
MsaG011213 MtrunA17_Chr7g0263531 52.857 70 32 1 162 231 352 420 7.20e-20 89.7
MsaG011213 MtrunA17_Chr7g0263531 53.333 60 26 2 172 231 190 247 9.65e-15 74.3
MsaG011213 MtrunA17_Chr5g0418191 54.688 64 28 1 169 232 318 380 8.57e-20 89.4
MsaG011213 MtrunA17_Chr5g0418191 45.455 77 32 4 172 246 152 220 6.04e-13 68.9
MsaG011213 MtrunA17_Chr3g0101131 57.812 64 26 1 169 232 413 475 8.78e-20 89.7
MsaG011213 MtrunA17_Chr3g0101131 48.333 60 29 2 172 231 244 301 1.49e-11 65.1
MsaG011213 MtrunA17_Chr4g0042831 42.056 107 46 3 137 243 259 349 9.39e-20 89.0
MsaG011213 MtrunA17_Chr4g0042831 48.529 68 34 1 172 239 146 212 3.43e-13 69.7
MsaG011213 MtrunA17_Chr5g0443131 50.649 77 35 2 156 232 144 217 1.21e-19 87.4
MsaG011213 MtrunA17_Chr1g0150801 50.667 75 34 2 156 230 89 160 3.40e-19 83.2
MsaG011213 MtrunA17_Chr3g0079691 41.667 96 40 2 137 232 476 555 5.45e-19 87.8
MsaG011213 MtrunA17_Chr3g0079691 34.783 115 47 4 171 285 280 366 1.98e-12 67.8
MsaG011213 MtrunA17_Chr7g0218081 56.667 60 25 1 169 228 408 466 6.22e-19 87.0
MsaG011213 MtrunA17_Chr7g0218081 43.373 83 43 3 172 252 229 309 1.50e-11 65.1
MsaG011213 MtrunA17_Chr5g0432161 40.741 108 48 3 125 232 390 481 6.41e-19 87.4
MsaG011213 MtrunA17_Chr5g0432161 50.794 63 29 2 172 234 245 305 4.01e-14 72.8
MsaG011213 MtrunA17_Chr5g0401821 40.385 104 55 2 125 228 160 256 7.76e-19 84.7
MsaG011213 MtrunA17_Chr7g0243891 38.318 107 50 2 126 232 344 434 8.76e-19 86.7
MsaG011213 MtrunA17_Chr7g0243891 45.714 70 36 2 171 240 216 283 2.60e-14 73.6
MsaG011213 MtrunA17_Chr5g0418781 50.685 73 33 2 156 228 111 180 1.05e-18 84.3
MsaG011213 MtrunA17_Chr8g0383351 49.315 73 35 2 156 228 146 216 1.07e-18 83.6
MsaG011213 MtrunA17_Chr7g0224801 38.095 126 64 3 115 229 28 150 1.50e-18 81.6
MsaG011213 MtrunA17_Chr7g0249311 44.545 110 54 4 126 232 347 452 1.67e-18 86.3
MsaG011213 MtrunA17_Chr7g0249311 49.231 65 29 3 171 233 179 241 1.05e-11 65.5
MsaG011213 MtrunA17_Chr7g0248721 51.282 78 34 3 152 229 124 197 2.01e-18 82.4
MsaG011213 MtrunA17_Chr7g0249301 43.750 96 51 3 139 232 258 352 4.03e-18 84.0
MsaG011213 MtrunA17_Chr7g0249301 46.774 62 31 2 170 231 61 120 4.19e-11 63.2
MsaG011213 MtrunA17_Chr1g0147881 44.048 84 43 1 149 228 236 319 4.39e-18 83.6
MsaG011213 MtrunA17_Chr4g0068541 49.351 77 36 2 155 231 194 267 4.43e-18 84.3
MsaG011213 MtrunA17_Chr3g0106491 39.091 110 51 3 123 232 332 425 5.19e-18 84.3
MsaG011213 MtrunA17_Chr3g0106491 50.000 60 28 2 172 231 199 256 4.66e-14 72.4
MsaG011213 MtrunA17_Chr8g0386241 44.872 78 42 1 169 246 101 177 6.45e-18 80.1
MsaG011213 MtrunA17_Chr4g0053281 56.140 57 25 0 172 228 267 323 9.99e-18 82.4
MsaG011213 MtrunA17_Chr3g0129321 30.570 193 110 6 88 273 30 205 1.02e-17 80.1
MsaG011213 MtrunA17_Chr5g0447701 50.000 78 36 2 155 232 238 312 1.10e-17 82.8
MsaG011213 MtrunA17_Chr3g0129361 47.945 73 35 2 156 228 131 200 2.07e-17 79.7
MsaG011213 MtrunA17_Chr8g0383251 47.368 76 37 2 156 231 95 167 6.39e-17 77.4
MsaG011213 MtrunA17_Chr8g0335811 39.130 115 60 4 115 228 105 210 8.15e-17 78.2
MsaG011213 MtrunA17_Chr2g0293481 43.038 79 45 0 168 246 264 342 8.82e-17 80.9
MsaG011213 MtrunA17_Chr1g0184691 51.667 60 29 0 169 228 291 350 1.18e-16 79.7
MsaG011213 MtrunA17_Chr7g0270051 35.238 105 65 1 135 239 20 121 1.36e-16 78.2
MsaG011213 MtrunA17_Chr7g0238921 45.333 75 30 2 172 236 125 198 4.32e-16 77.4
MsaG011213 MtrunA17_Chr4g0071521 45.570 79 42 1 151 228 224 302 5.09e-16 77.4
MsaG011213 MtrunA17_Chr8g0387501 46.774 62 33 0 172 233 130 191 1.12e-15 75.9
MsaG011213 MtrunA17_Chr8g0348311 37.895 95 53 2 172 266 156 244 1.22e-15 76.6
MsaG011213 MtrunA17_Chr7g0228941 43.939 66 37 0 163 228 44 109 1.22e-15 72.4
MsaG011213 MtrunA17_Chr8g0379461 35.870 92 59 0 140 231 45 136 4.95e-15 73.6
MsaG011213 MtrunA17_Chr4g0024391 38.462 78 46 1 155 232 215 290 4.96e-15 75.5
MsaG011213 MtrunA17_Chr3g0139021 31.818 110 58 2 124 228 274 371 5.88e-15 75.1
MsaG011213 MtrunA17_Chr4g0074001 43.836 73 40 1 159 231 347 418 6.02e-15 75.1
MsaG011213 MtrunA17_Chr7g0248691 43.077 65 37 0 169 233 152 216 9.04e-15 73.9
MsaG011213 MtrunA17_Chr5g0432071 39.394 99 52 4 172 269 105 196 2.96e-14 70.5
MsaG011213 MtrunA17_Chr2g0284321 45.000 60 33 0 172 231 36 95 3.99e-14 70.5
MsaG011213 MtrunA17_Chr1g0189391 32.800 125 70 5 111 228 37 154 6.03e-14 71.6
MsaG011213 MtrunA17_Chr2g0289181 45.161 62 34 0 171 232 14 75 6.74e-14 70.1
MsaG011213 MtrunA17_Chr2g0319961 36.364 110 66 2 125 231 32 140 1.12e-13 69.7
MsaG011213 MtrunA17_Chr6g0485431 38.202 89 50 4 149 235 133 218 1.36e-13 70.5
MsaG011213 MtrunA17_Chr1g0202461 43.548 62 35 0 172 233 262 323 7.81e-13 68.6
MsaG011213 MtrunA17_Chr5g0447711 38.667 75 45 1 158 232 217 290 2.15e-12 66.6
MsaG011213 MtrunA17_Chr8g0387981 47.059 68 32 2 163 226 122 189 7.35e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011213 AT1G80840.1 53.776 331 106 12 1 317 1 298 5.81e-99 294
MsaG011213 AT4G31800.1 44.940 336 136 10 1 316 1 307 6.68e-79 243
MsaG011213 AT4G31800.2 46.947 262 100 7 74 316 1 242 1.05e-67 212
MsaG011213 AT2G25000.2 45.660 265 94 8 57 314 2 223 2.19e-63 200
MsaG011213 AT2G25000.4 45.660 265 94 8 57 314 2 223 2.19e-63 200
MsaG011213 AT2G25000.1 44.765 277 99 8 49 316 37 268 2.58e-63 202
MsaG011213 AT2G25000.3 43.986 291 106 9 33 314 3 245 8.37e-63 199
MsaG011213 AT4G31800.3 46.053 228 88 8 1 208 1 213 1.25e-49 165
MsaG011213 AT5G41570.1 48.936 94 44 3 136 229 66 155 2.78e-23 95.1
MsaG011213 AT4G01720.1 25.143 350 152 9 60 310 105 443 4.92e-23 99.4
MsaG011213 AT4G18170.1 47.525 101 49 3 133 232 135 232 6.71e-23 97.1
MsaG011213 AT4G04450.2 39.252 107 56 1 125 231 161 258 8.69e-23 98.2
MsaG011213 AT1G64000.1 52.632 76 33 2 154 229 99 171 8.85e-23 94.0
MsaG011213 AT1G68150.1 51.852 81 34 2 151 231 220 295 1.26e-22 97.4
MsaG011213 AT4G22070.4 50.000 84 37 2 148 231 279 357 1.72e-22 98.2
MsaG011213 AT4G22070.3 50.000 84 37 2 148 231 279 357 1.72e-22 98.2
MsaG011213 AT4G22070.2 50.000 84 37 2 148 231 279 357 1.72e-22 98.2
MsaG011213 AT4G22070.1 50.000 84 37 2 148 231 279 357 1.72e-22 98.2
MsaG011213 AT4G04450.1 39.252 107 56 1 125 231 255 352 2.63e-22 97.4
MsaG011213 AT1G29860.1 52.439 82 36 2 156 237 123 201 2.97e-22 94.7
MsaG011213 AT1G62300.1 50.000 84 37 2 148 231 294 372 4.18e-22 97.1
MsaG011213 AT5G15130.2 38.793 116 55 3 121 231 141 245 1.11e-21 95.5
MsaG011213 AT5G15130.1 38.793 116 55 3 121 231 183 287 1.25e-21 95.5
MsaG011213 AT1G18860.1 58.065 62 26 0 170 231 238 299 1.78e-21 95.1
MsaG011213 AT5G07100.2 33.987 153 80 4 85 232 65 201 3.29e-21 90.5
MsaG011213 AT5G07100.2 45.455 77 41 1 159 235 12 87 3.65e-16 76.6
MsaG011213 AT5G07100.4 33.987 153 80 4 85 232 65 201 3.29e-21 90.5
MsaG011213 AT5G07100.4 45.455 77 41 1 159 235 12 87 3.65e-16 76.6
MsaG011213 AT5G07100.5 33.987 153 80 4 85 232 65 201 3.29e-21 90.5
MsaG011213 AT5G07100.5 45.455 77 41 1 159 235 12 87 3.65e-16 76.6
MsaG011213 AT5G07100.3 33.987 153 80 4 85 232 65 201 3.29e-21 90.5
MsaG011213 AT5G07100.3 45.455 77 41 1 159 235 12 87 3.65e-16 76.6
MsaG011213 AT2G47260.1 50.000 86 40 2 151 236 156 238 5.40e-21 92.0
MsaG011213 AT5G49520.1 51.948 77 34 2 155 231 207 280 6.14e-21 92.8
MsaG011213 AT5G07100.1 33.987 153 80 4 85 232 158 294 7.67e-21 91.3
MsaG011213 AT5G07100.1 45.455 77 41 1 159 235 105 180 1.22e-15 76.6
MsaG011213 AT3G62340.1 53.086 81 35 2 151 231 100 177 9.54e-21 90.5
MsaG011213 AT2G21900.1 44.554 101 48 3 133 232 76 169 1.31e-20 88.2
MsaG011213 AT4G26640.5 32.512 203 119 6 113 310 144 333 1.48e-20 91.7
MsaG011213 AT4G26640.5 43.662 71 38 2 161 231 25 93 3.33e-12 67.0
MsaG011213 AT4G26640.6 32.512 203 119 6 113 310 144 333 1.48e-20 91.7
MsaG011213 AT4G26640.6 43.662 71 38 2 161 231 25 93 3.33e-12 67.0
MsaG011213 AT4G26640.4 32.512 203 119 6 113 310 144 333 1.48e-20 91.7
MsaG011213 AT4G26640.4 43.662 71 38 2 161 231 25 93 3.33e-12 67.0
MsaG011213 AT4G26640.3 32.512 203 119 6 113 310 144 333 1.48e-20 91.7
MsaG011213 AT4G26640.3 43.662 71 38 2 161 231 25 93 3.33e-12 67.0
MsaG011213 AT5G26170.1 53.425 73 31 2 156 228 100 169 2.11e-20 87.0
MsaG011213 AT1G13960.1 34.694 147 68 3 101 247 366 484 2.41e-20 91.7
MsaG011213 AT1G13960.1 50.746 67 31 2 172 238 230 294 2.48e-13 70.9
MsaG011213 AT4G26640.1 32.512 203 119 6 113 310 248 437 3.53e-20 91.3
MsaG011213 AT4G26640.1 43.662 71 38 2 161 231 129 197 3.40e-12 67.4
MsaG011213 AT1G13960.2 34.694 147 68 3 101 247 213 331 4.03e-20 90.1
MsaG011213 AT1G13960.2 50.746 67 31 2 172 238 77 141 2.70e-13 70.1
MsaG011213 AT1G69310.4 50.000 78 36 2 151 228 129 203 5.26e-20 88.6
MsaG011213 AT1G69310.2 50.000 78 36 2 151 228 129 203 5.26e-20 88.6
MsaG011213 AT1G69310.1 50.000 78 36 2 151 228 129 203 5.26e-20 88.6
MsaG011213 AT4G26640.7 36.429 140 81 3 113 247 280 416 5.88e-20 90.5
MsaG011213 AT4G26640.7 43.662 71 38 2 161 231 161 229 3.86e-12 67.4
MsaG011213 AT4G26640.2 36.429 140 81 3 113 247 320 456 6.44e-20 90.5
MsaG011213 AT4G26640.2 43.662 71 38 2 161 231 201 269 3.93e-12 67.4
MsaG011213 AT5G46350.1 40.476 126 54 6 156 280 170 275 8.19e-20 88.6
MsaG011213 AT5G56270.1 57.812 64 26 1 169 232 485 547 9.00e-20 90.5
MsaG011213 AT5G56270.1 44.737 76 36 3 170 245 272 341 1.61e-12 68.6
MsaG011213 AT2G03340.1 56.250 64 27 1 169 232 413 475 9.67e-20 90.1
MsaG011213 AT2G03340.1 47.222 72 36 2 172 243 251 320 4.49e-14 73.2
MsaG011213 AT5G13080.1 50.000 80 37 2 156 235 54 130 1.83e-19 83.6
MsaG011213 AT1G55600.1 50.000 80 37 2 152 231 290 366 2.33e-19 88.6
MsaG011213 AT3G01080.2 56.667 60 25 1 169 228 342 400 1.16e-18 86.7
MsaG011213 AT3G01080.1 56.667 60 25 1 169 228 304 362 1.27e-18 86.3
MsaG011213 AT1G69810.1 29.365 252 113 8 43 231 15 264 1.27e-18 85.9
MsaG011213 AT2G30250.1 48.276 87 42 2 146 232 305 388 3.55e-18 84.7
MsaG011213 AT2G30250.1 35.577 104 52 4 172 262 167 268 1.56e-12 68.2
MsaG011213 AT5G64810.1 37.931 116 67 3 113 228 56 166 4.24e-18 81.3
MsaG011213 AT3G01970.1 51.351 74 33 2 155 228 51 121 6.54e-18 79.7
MsaG011213 AT5G52830.1 42.045 88 51 0 172 259 166 253 8.06e-18 83.2
MsaG011213 AT1G69810.2 48.750 80 38 2 153 231 129 206 8.49e-18 83.2
MsaG011213 AT2G23320.1 39.683 126 62 4 103 228 186 297 1.16e-17 82.4
MsaG011213 AT4G39410.1 45.783 83 42 2 146 228 200 279 1.48e-17 82.0
MsaG011213 AT2G46130.1 48.101 79 38 2 151 229 12 87 1.53e-17 77.4
MsaG011213 AT2G04880.1 50.704 71 34 1 169 239 305 374 1.82e-17 83.2
MsaG011213 AT2G04880.1 40.244 82 46 3 169 249 109 188 1.39e-12 68.6
MsaG011213 AT2G04880.2 50.704 71 34 1 169 239 281 350 2.09e-17 82.8
MsaG011213 AT2G04880.2 40.244 82 46 3 169 249 109 188 1.58e-12 68.2
MsaG011213 AT2G38470.1 53.125 64 29 1 169 232 360 422 2.95e-17 82.8
MsaG011213 AT2G38470.1 39.806 103 54 3 171 273 184 278 1.91e-14 74.3
MsaG011213 AT4G26440.1 54.688 64 28 1 169 232 370 432 4.11e-17 82.4
MsaG011213 AT4G26440.1 44.928 69 32 3 172 240 179 241 1.77e-11 65.5
MsaG011213 AT2G30590.1 50.746 67 32 1 169 234 311 377 4.42e-17 81.6
MsaG011213 AT2G44745.1 38.655 119 68 4 120 236 95 210 4.63e-17 79.0
MsaG011213 AT4G31550.2 51.667 60 29 0 169 228 243 302 5.04e-17 80.9
MsaG011213 AT4G31550.1 51.667 60 29 0 169 228 244 303 5.14e-17 80.9
MsaG011213 AT1G29280.1 50.000 60 30 0 172 231 75 134 6.84e-17 79.3
MsaG011213 AT5G43290.1 42.857 84 46 2 145 228 89 170 7.16e-17 79.7
MsaG011213 AT2G34830.1 35.043 117 70 1 123 233 161 277 8.36e-17 80.9
MsaG011213 AT2G34830.2 35.043 117 70 1 123 233 170 286 1.01e-16 80.9
MsaG011213 AT3G04670.1 51.667 60 29 0 169 228 260 319 2.97e-16 78.6
MsaG011213 AT4G24240.1 44.304 79 43 1 151 228 260 338 4.41e-16 78.2
MsaG011213 AT2G37260.3 37.500 120 64 3 113 228 213 325 4.94e-16 78.2
MsaG011213 AT2G37260.3 48.000 75 37 2 158 232 72 144 3.97e-14 72.4
MsaG011213 AT1G69310.3 48.718 78 33 3 151 228 129 199 5.06e-16 77.4
MsaG011213 AT2G37260.2 37.500 120 64 3 113 228 211 323 5.36e-16 78.2
MsaG011213 AT2G37260.2 48.000 75 37 2 158 232 70 142 4.18e-14 72.4
MsaG011213 AT2G37260.1 37.037 135 69 4 113 238 293 420 7.40e-16 78.2
MsaG011213 AT2G37260.1 48.000 75 37 2 158 232 152 224 4.41e-14 72.8
MsaG011213 AT5G28650.1 51.667 60 29 0 169 228 260 319 8.29e-16 77.4
MsaG011213 AT1G30650.1 45.714 70 38 0 164 233 210 279 8.70e-16 77.8
MsaG011213 AT2G24570.1 50.000 60 30 0 169 228 241 300 1.40e-15 76.6
MsaG011213 AT5G45050.1 37.500 120 66 2 172 282 1181 1300 1.56e-15 77.8
MsaG011213 AT5G45050.2 37.500 120 66 2 172 282 1153 1272 1.64e-15 77.8
MsaG011213 AT5G45050.4 37.500 120 66 2 172 282 1042 1161 1.83e-15 77.8
MsaG011213 AT3G58710.2 40.323 124 64 3 129 245 24 144 2.17e-15 75.5
MsaG011213 AT4G01250.1 38.824 85 52 0 172 256 129 213 2.37e-15 75.5
MsaG011213 AT3G58710.1 43.243 74 42 0 172 245 72 145 3.12e-15 74.7
MsaG011213 AT4G23550.1 32.192 146 87 4 169 314 138 271 3.81e-15 75.1
MsaG011213 AT4G30935.1 42.466 73 41 1 159 231 319 390 4.79e-15 75.9
MsaG011213 AT2G46130.2 46.835 79 35 3 151 229 12 83 1.20e-13 66.6
MsaG011213 AT2G46400.1 41.096 73 39 2 156 226 91 161 9.54e-12 65.1
MsaG011213 AT5G45260.1 37.975 79 47 1 172 248 1209 1287 1.47e-11 65.9
MsaG011213 AT3G01080.3 40.000 75 43 2 172 246 206 278 7.53e-11 62.8

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTGTGGTACTTCCATTT+TGG 0.140306 2:-82734114 None:intergenic
AATGGAAGTACCACAGATTT+TGG 0.248540 2:+82734118 MsaT011213.1:CDS
TCATACTTCTTGAAGCTTAC+AGG 0.255823 2:-82733130 None:intergenic
GCACTTGCTGCTGCCATATC+AGG 0.283466 2:+82734188 MsaT011213.1:CDS
AATCTAATGGGAGGAGTAAC+TGG 0.300205 2:+82733526 MsaT011213.1:CDS
GGATATCAGTGGAGAAAATA+TGG 0.332364 2:+82733795 MsaT011213.1:CDS
GATGATAAATAATCCCAAAA+TGG 0.343074 2:+82734100 MsaT011213.1:CDS
TTTATCATCATATTCTTAGA+AGG 0.347068 2:-82734086 None:intergenic
AATAGTAGAAACTTGCATTA+AGG 0.347618 2:+82733606 MsaT011213.1:CDS
CAAGTACAGTTACACTTGAT+TGG 0.350481 2:+82734039 MsaT011213.1:CDS
AAGCAGCAGTAACAATCTAA+TGG 0.359770 2:+82733513 MsaT011213.1:CDS
AAGGTACTACTGGACTAATT+TGG 0.361772 2:-82734007 None:intergenic
TTCTTCTTCACTGTGCAGCT+TGG 0.402986 2:-82733876 None:intergenic
AGCATTTGAAGTATGCTCTT+GGG 0.421783 2:-82733845 None:intergenic
TTCCTACAACGATTGTTTGT+CGG 0.422479 2:-82733984 None:intergenic
CAAGTTTCTACTATTTGCCT+TGG 0.425526 2:-82733598 None:intergenic
GGGCTTTGCTCCTTATGATC+AGG 0.429712 2:-82733463 None:intergenic
GAGAAAGAATCCTGATCATA+AGG 0.436763 2:+82733453 MsaT011213.1:CDS
AGCAGCAGTAACAATCTAAT+GGG 0.440609 2:+82733514 MsaT011213.1:CDS
TCAGTCAAGTGGTGCCTTGG+TGG 0.446693 2:+82733327 MsaT011213.1:intron
GAGCATTTGAAGTATGCTCT+TGG 0.457888 2:-82733846 None:intergenic
ATCATGGATTAACACTTCCT+TGG 0.460598 2:+82733066 MsaT011213.1:CDS
GTGGAGAAAATATGGACAAA+AGG 0.461765 2:+82733803 MsaT011213.1:CDS
TTGAAGTATGCTCTTGGGGA+TGG 0.464325 2:-82733840 None:intergenic
TTCTTAGAAGGTTCAGAAGA+AGG 0.464649 2:-82734074 None:intergenic
CAATCAAGTGTAACTGTACT+TGG 0.475314 2:-82734038 None:intergenic
AGCAAAGCCCATCTCAGAAA+AGG 0.487615 2:+82733476 MsaT011213.1:CDS
GTCAAGTGGTGCCTTGGTGG+AGG 0.490012 2:+82733330 MsaT011213.1:intron
TGCTCTGAAGTTAAGATCCT+TGG 0.493603 2:-82734165 None:intergenic
TCATCCTCATCCTTCTCAAA+CGG 0.516116 2:+82733959 MsaT011213.1:CDS
GCAGAAAACAAGAAGTTGAC+AGG 0.521855 2:+82733370 MsaT011213.1:CDS
TATGTTGCAACCACAACTGA+TGG 0.530178 2:-82733921 None:intergenic
CACATTGAGAAACAATCTGA+TGG 0.530744 2:+82733423 MsaT011213.1:CDS
GGTGCAGAAGAAGGTACTAC+TGG 0.531405 2:-82734017 None:intergenic
TGTAAGCTTCAAGAAGTATG+AGG 0.535043 2:+82733132 MsaT011213.1:CDS
ACTGTACTTGGTGCAGAAGA+AGG 0.542764 2:-82734026 None:intergenic
CAACATTAATCCACGCAGTA+GGG 0.544477 2:+82733093 MsaT011213.1:CDS
TCAACATTAATCCACGCAGT+AGG 0.551260 2:+82733092 MsaT011213.1:CDS
GTTGTGGTTGCAACATATGA+AGG 0.552701 2:+82733927 MsaT011213.1:CDS
AGCCGACAAACAATCGTTGT+AGG 0.557166 2:+82733982 MsaT011213.1:CDS
TATATCTTCTGCAAAAGAAG+AGG 0.565925 2:+82733186 MsaT011213.1:CDS
TTTGTCGGCTCCGTTTGAGA+AGG 0.566050 2:-82733969 None:intergenic
TGGATTAATGTTGAGATCCA+AGG 0.586656 2:-82733083 None:intergenic
GAAGAAGGTGCAAAGAAGTG+TGG 0.601905 2:+82733893 MsaT011213.1:CDS
ATATGGACAAAAGGTGACAA+GGG 0.608161 2:+82733812 MsaT011213.1:CDS
ATCAATCATGCAAGAAACCA+AGG 0.612418 2:+82733581 MsaT011213.1:CDS
GCATTTGAAGTATGCTCTTG+GGG 0.616672 2:-82733844 None:intergenic
TACTTCTTGAAGCTTACAGG+AGG 0.621579 2:-82733127 None:intergenic
AATATGGACAAAAGGTGACA+AGG 0.623618 2:+82733811 MsaT011213.1:CDS
ATCTGTTCCACCAAAATCTG+TGG 0.633715 2:-82734128 None:intergenic
GGCTCCGTTTGAGAAGGATG+AGG 0.643319 2:-82733963 None:intergenic
AAGCTTCAAGAAGTATGAGG+TGG 0.647098 2:+82733135 MsaT011213.1:CDS
TTGTGAAAGATGGATATCAG+TGG 0.648462 2:+82733784 MsaT011213.1:CDS
TGTGGATGATCCATCAGTTG+TGG 0.652865 2:+82733911 MsaT011213.1:CDS
AGCAGTAACAATCTAATGGG+AGG 0.658927 2:+82733517 MsaT011213.1:CDS
AAGCTGCACAGTGAAGAAGA+AGG 0.665099 2:+82733878 MsaT011213.1:CDS
GATGGCTACTTCATTGACCA+AGG 0.672567 2:+82734148 MsaT011213.1:CDS
TGTTCATGAACCCTACTGCG+TGG 0.674456 2:-82733103 None:intergenic
TCGTTTCAGCTCCTCCACCA+AGG 0.688042 2:-82733341 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 82733052 82734232 82733052 ID=MsaG011213
Chr2 mRNA 82733052 82734232 82733052 ID=MsaT011213.1;Parent=MsaG011213
Chr2 exon 82733052 82733207 82733052 ID=MsaT011213.1.exon1;Parent=MsaT011213.1
Chr2 CDS 82733052 82733207 82733052 ID=cds.MsaT011213.1;Parent=MsaT011213.1
Chr2 exon 82733331 82733678 82733331 ID=MsaT011213.1.exon2;Parent=MsaT011213.1
Chr2 CDS 82733331 82733678 82733331 ID=cds.MsaT011213.1;Parent=MsaT011213.1
Chr2 exon 82733783 82734232 82733783 ID=MsaT011213.1.exon3;Parent=MsaT011213.1
Chr2 CDS 82733783 82734232 82733783 ID=cds.MsaT011213.1;Parent=MsaT011213.1
Gene Sequence

>MsaT011213.1

ATGGATTTTTCATCATCATGGATTAACACTTCCTTGGATCTCAACATTAATCCACGCAGTAGGGTTCATGAACAACCTCCTGTAAGCTTCAAGAAGTATGAGGTGGAAAACAATTTTTTCTCATTAGGGATGAGTATATCTTCTGCAAAAGAAGAGTCAAGTGGTGCCTTGGTGGAGGAGCTGAAACGAGTGAGTGCAGAAAACAAGAAGTTGACAGGAATGTTGACTGAGATGTGTGAGAATTACAACACATTGAGAAACAATCTGATGGAATATATGAGAAAGAATCCTGATCATAAGGAGCAAAGCCCATCTCAGAAAAGGAAATCTGATGAAATAAGCAGCAGTAACAATCTAATGGGAGGAGTAACTGGAAACAACTCTGAGAGCAGTTCAAGTGATGAAGATCAATCATGCAAGAAACCAAGGCAAATAGTAGAAACTTGCATTAAGGCTAAAGTTTCAAGAGTTTATTTCAAGACAGAATCATCTGATTCAAGCCTTATTGTGAAAGATGGATATCAGTGGAGAAAATATGGACAAAAGGTGACAAGGGATAATCCATCCCCAAGAGCATACTTCAAATGCTCATTTGCTCCAAGCTGCACAGTGAAGAAGAAGGTGCAAAGAAGTGTGGATGATCCATCAGTTGTGGTTGCAACATATGAAGGAGAGCATAATCATCCTCATCCTTCTCAAACGGAGCCGACAAACAATCGTTGTAGGAACCAAATTAGTCCAGTAGTACCTTCTTCTGCACCAAGTACAGTTACACTTGATTGGACAAAGAAAGCACCTTCTTCTGAACCTTCTAAGAATATGATGATAAATAATCCCAAAATGGAAGTACCACAGATTTTGGTGGAACAGATGGCTACTTCATTGACCAAGGATCTTAACTTCAGAGCAGCACTTGCTGCTGCCATATCAGGAAAAATGTTACACCAAAATTAG
Protein sequence

>MsaT011213.1

MDFSSSWINTSLDLNINPRSRVHEQPPVSFKKYEVENNFFSLGMSISSAKEESSGALVEELKRVSAENKKLTGMLTEMCENYNTLRNNLMEYMRKNPDHKEQSPSQKRKSDEISSSNNLMGGVTGNNSESSSSDEDQSCKKPRQIVETCIKAKVSRVYFKTESSDSSLIVKDGYQWRKYGQKVTRDNPSPRAYFKCSFAPSCTVKKKVQRSVDDPSVVVATYEGEHNHPHPSQTEPTNNRCRNQISPVVPSSAPSTVTLDWTKKAPSSEPSKNMMINNPKMEVPQILVEQMATSLTKDLNFRAALAAAISGKMLHQN*