AlfalfaGEDB | MsaG011229

M. sativa ssp. caerulea / MsaG011229

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
PFAM: PF01167.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011229	XP_013465630.1	86.614	381	16	7	1	366	1	361	0.0	631

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011229	sp\|Q9S9M8\|TLP8_ARATH	56.839	329	125	6	50	366	74	397	6.00e-127	373

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011229	tr\|G7ZYZ4\|G7ZYZ4_MEDTR	86.614	381	16	7	1	366	1	361	0.0	631

TFs/TRs:

Gene ID	Type	Classification
MsaG011229	TF	TUB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG002826	MsaG011229	0.822284	4.529416e-53	1.058447e-50
MsaG005330	MsaG011229	0.814850	2.159677e-51	4.159062e-49
MsaG008892	MsaG011229	0.816550	9.063434e-52	1.822389e-49
MsaG010332	MsaG011229	0.805010	2.772694e-49	4.204579e-47
MsaG010334	MsaG011229	0.805677	2.012867e-49	3.100035e-47
MsaG011229	MsaG011413	0.807339	9.009539e-50	1.443140e-47
MsaG011229	MsaG020193	0.817440	5.733509e-52	1.179579e-49
MsaG011229	MsaG021799	0.833029	1.221877e-55	3.860367e-53
MsaG011229	MsaG028557	0.824130	1.687563e-53	4.145493e-51
MsaG011229	MsaG031615	0.833859	7.600089e-56	2.460421e-53
MsaG011229	MsaG032531	0.818840	2.774561e-52	5.918717e-50
MsaG011229	MsaG033878	0.834975	4.000471e-56	1.338991e-53
MsaG011229	MsaG034933	0.849586	5.588443e-60	2.986691e-57
MsaG011229	MsaG040643	0.845308	8.262555e-59	3.824425e-56
MsaG011229	MsaG041557	0.838233	5.970499e-57	2.205505e-54
MsaG011229	MsaG041550	0.823306	2.626009e-53	6.308191e-51
MsaG011229	MsaG044703	0.856193	7.365600e-62	4.969025e-59
MsaG011229	MsaG045348	0.816372	9.932085e-52	1.987979e-49
MsaG011229	MsaG002656	0.808208	5.903113e-50	9.654555e-48
MsaG011229	MsaG018616	0.845549	7.113416e-59	3.318999e-56
MsaG011229	MsaG029709	0.803206	6.550613e-49	9.526966e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011229	MtrunA17_Chr2g0334041	86.614	381	16	7	1	366	1	361	0.0	631
MsaG011229	MtrunA17_Chr1g0155301	28.253	269	126	12	150	364	166	421	5.71e-14	72.8
MsaG011229	MtrunA17_Chr2g0286441	40.000	100	48	4	272	364	298	392	1.05e-12	68.9
MsaG011229	MtrunA17_Chr1g0196071	50.000	62	24	1	310	364	340	401	1.96e-12	68.2
MsaG011229	MtrunA17_Chr2g0278511	40.909	110	44	5	270	364	323	426	3.24e-12	67.4
MsaG011229	MtrunA17_Chr5g0404321	38.947	95	44	3	279	364	326	415	5.86e-12	66.6
MsaG011229	MtrunA17_Chr8g0373001	36.697	109	54	4	265	364	309	411	1.58e-11	65.1
MsaG011229	MtrunA17_Chr1g0201891	36.207	116	47	4	270	364	321	430	1.88e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011229	AT1G16070.1	56.839	329	125	6	50	366	74	397	6.10e-128	373
MsaG011229	AT1G16070.2	54.804	281	110	6	50	318	74	349	4.81e-98	296
MsaG011229	AT1G16070.3	54.054	222	85	6	50	259	74	290	5.95e-73	229
MsaG011229	AT2G18280.2	25.797	345	177	13	87	364	60	392	8.71e-16	78.6
MsaG011229	AT2G18280.1	25.797	345	177	13	87	364	60	392	8.71e-16	78.6
MsaG011229	AT2G47900.2	53.226	62	22	2	310	364	344	405	2.69e-12	68.2
MsaG011229	AT2G47900.1	53.226	62	22	2	310	364	344	405	2.69e-12	68.2
MsaG011229	AT2G47900.3	53.226	62	22	2	310	364	345	406	2.73e-12	68.2
MsaG011229	AT1G25280.3	31.884	138	69	3	247	364	134	266	3.84e-12	66.2
MsaG011229	AT1G25280.2	31.884	138	69	3	247	364	134	266	3.84e-12	66.2
MsaG011229	AT1G47270.3	26.923	260	133	11	150	364	140	387	4.20e-12	67.4
MsaG011229	AT1G47270.1	26.923	260	133	11	150	364	165	412	4.25e-12	67.4
MsaG011229	AT1G47270.2	26.923	260	133	11	150	364	164	411	4.52e-12	67.4
MsaG011229	AT1G25280.1	31.884	138	69	3	247	364	312	444	1.14e-11	66.2
MsaG011229	AT3G06380.2	45.902	61	27	1	310	364	227	287	6.31e-11	63.2

Find 106 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCCAGCTCTTTGAAAATT+TGG	0.061965	2:-82930504	MsaT011229.1:CDS
GTTGAAAATGAGTTGGATTT+TGG	0.248838	2:+82930610	None:intergenic
GTTTACTACTGCGGGGCTTA+TGG	0.274791	2:+82930017	None:intergenic
TTCCAGCTCTTTGAAAATTT+GGG	0.299500	2:-82930503	MsaT011229.1:CDS
AAACTTTCAGCTCACGTTAG+AGG	0.301321	2:-82929402	MsaT011229.1:intron
CCTGTACATGTTGCTATTGT+AGG	0.305231	2:+82929888	None:intergenic
GGTATAAAGAGAGAAGAAAT+TGG	0.306355	2:+82930333	None:intergenic
TACGACCAAGGGTTTGGTTT+TGG	0.321955	2:+82930734	None:intergenic
CCGGTTCGAAATACCACATT+TGG	0.324387	2:-82930057	MsaT011229.1:CDS
GAGCTTGACTATAGAGATAA+AGG	0.329596	2:-82929458	MsaT011229.1:CDS
GGTTCAGGTACAACACATTA+AGG	0.334401	2:-82929670	MsaT011229.1:intron
ATTATTATTGTAGGAAGGTC+AGG	0.351343	2:-82930227	MsaT011229.1:intron
AGACCATTGCCTATAGATAT+TGG	0.366407	2:-82930409	MsaT011229.1:CDS
TTAAGGGACTGCCAATGAAT+TGG	0.380340	2:-82929653	MsaT011229.1:CDS
GCAACAAAGCTTTGCATCAA+TGG	0.384089	2:+82929076	None:intergenic
TCCCACTCCGGCGCTTGTGG+TGG	0.398163	2:+82930176	None:intergenic
GTGTACTTGTGTTATTGTTA+AGG	0.401096	2:-82930383	MsaT011229.1:CDS
ACCTCCCACTCCGGCGCTTG+TGG	0.404570	2:+82930173	None:intergenic
GAAATACCACATTTGGGATC+AGG	0.408006	2:-82930050	MsaT011229.1:CDS
AAAGACATAGATTGATGATT+GGG	0.409134	2:+82929837	None:intergenic
AAAACATACGACCAAGGGTT+TGG	0.409386	2:+82930728	None:intergenic
ATATCCTTTGACTGGTTACC+AGG	0.411071	2:-82929120	MsaT011229.1:CDS
TTCCTACCCTCCCTAGCTGC+AGG	0.422680	2:+82929262	None:intergenic
CTGCTGCTCCTGCTTCCTCC+TGG	0.428096	2:-82930462	MsaT011229.1:CDS
TGTTAAGGAACCAACTCCTC+AGG	0.436248	2:-82930368	MsaT011229.1:CDS
GAAAGACATAGATTGATGAT+TGG	0.438091	2:+82929836	None:intergenic
TCTACATATCAAAGACTTGT+TGG	0.438291	2:+82930429	None:intergenic
GTACACCTTCCAATATCTAT+AGG	0.439094	2:+82930400	None:intergenic
TAAGGGACTGCCAATGAATT+GGG	0.444518	2:-82929652	MsaT011229.1:CDS
CGGTTCGAAATACCACATTT+GGG	0.453880	2:-82930056	MsaT011229.1:CDS
GTTTGCTATGATTAGGAGAA+TGG	0.459646	2:-82929297	MsaT011229.1:intron
GATAAAGGAAGGCCAGGACT+TGG	0.466820	2:-82929443	MsaT011229.1:CDS
AAGAGGTGGTTTACTACTGC+GGG	0.474800	2:+82930009	None:intergenic
GGGACGGCAGAATAGGAAAC+TGG	0.475585	2:-82930206	MsaT011229.1:CDS
AAGGTCAGGGACGGCAGAAT+AGG	0.479149	2:-82930213	MsaT011229.1:CDS
CCCACTCCGGCGCTTGTGGT+GGG	0.479209	2:+82930177	None:intergenic
GAAGAATCAGAAGAAGGAAT+AGG	0.486222	2:+82930532	None:intergenic
CTACATATCAAAGACTTGTT+GGG	0.489447	2:+82930430	None:intergenic
GTATAAAGAGAGAAGAAATT+GGG	0.490055	2:+82930334	None:intergenic
CGTGTTTGTTGAAAATGAGT+TGG	0.490098	2:+82930603	None:intergenic
ATCTGCAGATATCCTTTGAC+TGG	0.494012	2:-82929128	MsaT011229.1:intron
CCAACTCCTCAGGGTCTCTC+CGG	0.498571	2:-82930358	MsaT011229.1:CDS
CCAAATGTGGTATTTCGAAC+CGG	0.504282	2:+82930057	None:intergenic
CCGGAGAGACCCTGAGGAGT+TGG	0.514963	2:+82930358	None:intergenic
TTTCGAACCGGTGAGGTTAG+CGG	0.516957	2:+82930069	None:intergenic
AAATACCACATTTGGGATCA+GGG	0.518347	2:-82930049	MsaT011229.1:CDS
AAAGTAGACCAGGAGGAAGC+AGG	0.520011	2:+82930454	None:intergenic
AGCAGACAATCCTGCAGCTA+GGG	0.521878	2:-82929272	MsaT011229.1:CDS
GGAAGCCAAACATATGCAAA+AGG	0.524760	2:+82929097	None:intergenic
TTTCAAAGAGCTGGAAAAGT+GGG	0.527227	2:+82930510	None:intergenic
CCTCCTACCTTGTTATAGAG+TGG	0.527819	2:+82929588	None:intergenic
TATAGAGATAAAGGAAGGCC+AGG	0.527875	2:-82929449	MsaT011229.1:CDS
AAGCAGACAATCCTGCAGCT+AGG	0.533935	2:-82929273	MsaT011229.1:CDS
CTTGTTGGGTAAAGTAGACC+AGG	0.539180	2:+82930444	None:intergenic
TTTGCTATGATTAGGAGAAT+GGG	0.539684	2:-82929296	MsaT011229.1:intron
AAGTGGGAAGAATCAGAAGA+AGG	0.539717	2:+82930526	None:intergenic
CTGTGGCCCACCACAAGCGC+CGG	0.547264	2:-82930183	MsaT011229.1:CDS
AAGTGCATCAACTATTCTCA+AGG	0.551216	2:-82929614	MsaT011229.1:CDS
GTTCAGGTACAACACATTAA+GGG	0.551587	2:-82929669	MsaT011229.1:intron
GATCTCTGGATTCCAAGTCC+TGG	0.553307	2:+82929431	None:intergenic
AAGAGAGAAGAAATTGGGAC+CGG	0.559507	2:+82930339	None:intergenic
AGACAATCCTGCAGCTAGGG+AGG	0.560928	2:-82929269	MsaT011229.1:CDS
GTCTTTCAAGAACACCAACC+AGG	0.561985	2:-82929820	MsaT011229.1:intron
CCTACAATAGCAACATGTAC+AGG	0.562677	2:-82929888	MsaT011229.1:CDS
TTATTATTGTAGGAAGGTCA+GGG	0.564327	2:-82930226	MsaT011229.1:intron
ACCACAAGCGCCGGAGTGGG+AGG	0.564951	2:-82930174	MsaT011229.1:CDS
CCCACCACAAGCGCCGGAGT+GGG	0.568859	2:-82930177	MsaT011229.1:CDS
GTTGGTGTCAATCTCGCTGA+GGG	0.574343	2:+82930699	None:intergenic
GACAATCCTGCAGCTAGGGA+GGG	0.574505	2:-82929268	MsaT011229.1:CDS
CCAAACATATGCAAAAGGCC+TGG	0.577415	2:+82929102	None:intergenic
AGCTTTGTTGCATAGTGTAG+TGG	0.581324	2:-82929064	None:intergenic
GCCCACCACAAGCGCCGGAG+TGG	0.582189	2:-82930178	MsaT011229.1:CDS
ACAGTTACCGCTAACCTCAC+CGG	0.586784	2:-82930076	MsaT011229.1:CDS
AGGAGCAGCAGCTTCAGAGT+CGG	0.586966	2:+82930474	None:intergenic
ATTTGAAAACATACGACCAA+GGG	0.596408	2:+82930723	None:intergenic
GTTAAGGAACCAACTCCTCA+GGG	0.602181	2:-82930367	MsaT011229.1:CDS
CATTGCCTATAGATATTGGA+AGG	0.608050	2:-82930405	MsaT011229.1:CDS
TGAAGTGAGACCTCCCACTC+CGG	0.610091	2:+82930164	None:intergenic
ACAGTAGCTCAGAATCTCAA+AGG	0.612108	2:-82930142	MsaT011229.1:CDS
TAGCTGCAGCAACAAGAACA+AGG	0.612300	2:-82930656	MsaT011229.1:CDS
AAGGCCTGGTAACCAGTCAA+AGG	0.614442	2:+82929116	None:intergenic
TACTCAACAACAGCAAGAGG+TGG	0.614584	2:+82929995	None:intergenic
CTTCCAATATCTATAGGCAA+TGG	0.616470	2:+82930406	None:intergenic
ATGAATCTAACTAACCTGGT+TGG	0.618468	2:+82929806	None:intergenic
GCAGAATAGGAAACTGGCTG+TGG	0.618843	2:-82930200	MsaT011229.1:CDS
ATCCTGCAGCTAGGGAGGGT+AGG	0.625245	2:-82929264	MsaT011229.1:CDS
ATGCAGAACTGTAATGAGGA+AGG	0.628521	2:+82930568	None:intergenic
TTGACTATAGAGATAAAGGA+AGG	0.630332	2:-82929454	MsaT011229.1:CDS
GCTTACTCAACAACAGCAAG+AGG	0.633778	2:+82929992	None:intergenic
GGACTGCCAATGAATTGGGA+AGG	0.634626	2:-82929648	MsaT011229.1:CDS
CAAGAGGTGGTTTACTACTG+CGG	0.635758	2:+82930008	None:intergenic
AGTGCATCAACTATTCTCAA+GGG	0.640841	2:-82929613	MsaT011229.1:CDS
GATATCCCTGATCCCAAATG+TGG	0.643558	2:+82930044	None:intergenic
CAGCAGCTTCAGAGTCGGAG+TGG	0.644616	2:+82930479	None:intergenic
TATTGTAGGAAGGTCAGGGA+CGG	0.654971	2:-82930222	MsaT011229.1:intron
TGTGGTATTTCGAACCGGTG+AGG	0.659341	2:+82930062	None:intergenic
CGTTGGTGTCAATCTCGCTG+AGG	0.660418	2:+82930698	None:intergenic
TTGACACCAACGTTCAGCTG+AGG	0.660689	2:-82930687	MsaT011229.1:CDS
TTGCATGCAGAACTGTAATG+AGG	0.667955	2:+82930564	None:intergenic
GATTTGAAAACATACGACCA+AGG	0.673412	2:+82930722	None:intergenic
AAGGGATCCACTCTATAACA+AGG	0.674607	2:-82929595	MsaT011229.1:intron
ACTATGCCTCAGCTGAACGT+TGG	0.687057	2:+82930681	None:intergenic
AGAGGTGGTTTACTACTGCG+GGG	0.697959	2:+82930010	None:intergenic
TTGGGACCGGAGAGACCCTG+AGG	0.711109	2:+82930352	None:intergenic
TTGGTGTCAATCTCGCTGAG+GGG	0.726946	2:+82930700	None:intergenic
GTTGGGTAAAGTAGACCAGG+AGG	0.744497	2:+82930447	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	82929067	82930767	82929067	ID=MsaG011229
Chr2	mRNA	82929067	82930767	82929067	ID=MsaT011229.1;Parent=MsaG011229
Chr2	exon	82929067	82929142	82929067	ID=MsaT011229.1.exon7;Parent=MsaT011229.1
Chr2	CDS	82929067	82929142	82929067	ID=cds.MsaT011229.1;Parent=MsaT011229.1
Chr2	exon	82929237	82929304	82929237	ID=MsaT011229.1.exon6;Parent=MsaT011229.1
Chr2	CDS	82929237	82929304	82929237	ID=cds.MsaT011229.1;Parent=MsaT011229.1
Chr2	exon	82929403	82929495	82929403	ID=MsaT011229.1.exon5;Parent=MsaT011229.1
Chr2	CDS	82929403	82929495	82929403	ID=cds.MsaT011229.1;Parent=MsaT011229.1
Chr2	exon	82929596	82929685	82929596	ID=MsaT011229.1.exon4;Parent=MsaT011229.1
Chr2	CDS	82929596	82929685	82929596	ID=cds.MsaT011229.1;Parent=MsaT011229.1
Chr2	exon	82929821	82929917	82929821	ID=MsaT011229.1.exon3;Parent=MsaT011229.1
Chr2	CDS	82929821	82929917	82929821	ID=cds.MsaT011229.1;Parent=MsaT011229.1
Chr2	exon	82929998	82930236	82929998	ID=MsaT011229.1.exon2;Parent=MsaT011229.1
Chr2	CDS	82929998	82930236	82929998	ID=cds.MsaT011229.1;Parent=MsaT011229.1
Chr2	exon	82930330	82930767	82930330	ID=MsaT011229.1.exon1;Parent=MsaT011229.1
Chr2	CDS	82930330	82930767	82930330	ID=cds.MsaT011229.1;Parent=MsaT011229.1

Gene Sequence

>MsaT011229.1

ATGAAGAAAAACCAAAACCAAACCCTTGGTCGTATGTTTTCAAATCCCCTCAGCGAGATTGACACCAACGTTCAGCTGAGGCATAGTCGTAGCTGCAGCAACAAGAACAAGGAAAATGCCAATGTTGTTTCTCTTCCAAAATCCAACTCATTTTCAACAAACACGAGGATTCTTAAGCCTTCCTCATTACAGTTCTGCATGCAAATCAACGATCCTATTCCTTCTTCTGATTCTTCCCACTTTTCCAGCTCTTTGAAAATTTGGGACCACTCCGACTCTGAAGCTGCTGCTCCTGCTTCCTCCTGGTCTACTTTACCCAACAAGTCTTTGATATGTAGACCATTGCCTATAGATATTGGAAGGTGTACTTGTGTTATTGTTAAGGAACCAACTCCTCAGGGTCTCTCCGGTCCCAATTTCTTCTCTCTTTATACCTATGAAGGTCAGGGACGGCAGAATAGGAAACTGGCTGTGGCCCACCACAAGCGCCGGAGTGGGAGGTCTCACTTCACAGTAGCTCAGAATCTCAAAGGACTACTCTCCAATTCTGATGATGATGATGATACTTTTCTCGGTACAGTTACCGCTAACCTCACCGGTTCGAAATACCACATTTGGGATCAGGGATATCATCGCCATAAGCCCCGCAGTAGTAAACCACCTCTTGCTGTTGTTGAGTATGTACCTACAATAGCAACATGTACAGGAACTCACAGAAGCATCAAAGCTTATATACCCAATCATCAATCTATGTCTTTCAAGAACACCAACCAGGTACAACACATTAAGGGACTGCCAATGAATTGGGAAGGAAAAATGGACAAAGTGCATCAACTATTCTCAAGGGATCCACTCTATAACAAGAGTACAAAGCAATTTGAGCTTGACTATAGAGATAAAGGAAGGCCAGGACTTGGAATCCAGAGATCCGTTAAAAACTTTCAGCTCACGTTAGAGGAGAATGGGAAGCAGACAATCCTGCAGCTAGGGAGGGTAGGAAAATCTAATTTTGTTATGGACTACAGATATCCTTTGACTGGTTACCAGGCCTTTTGCATATGTTTGGCTTCCATTGATGCAAAGCTTTGTTGCATAGTGTAG

Protein sequence

>MsaT011229.1

MKKNQNQTLGRMFSNPLSEIDTNVQLRHSRSCSNKNKENANVVSLPKSNSFSTNTRILKPSSLQFCMQINDPIPSSDSSHFSSSLKIWDHSDSEAAAPASSWSTLPNKSLICRPLPIDIGRCTCVIVKEPTPQGLSGPNFFSLYTYEGQGRQNRKLAVAHHKRRSGRSHFTVAQNLKGLLSNSDDDDDTFLGTVTANLTGSKYHIWDQGYHRHKPRSSKPPLAVVEYVPTIATCTGTHRSIKAYIPNHQSMSFKNTNQVQHIKGLPMNWEGKMDKVHQLFSRDPLYNKSTKQFELDYRDKGRPGLGIQRSVKNFQLTLEENGKQTILQLGRVGKSNFVMDYRYPLTGYQAFCICLASIDAKLCCIV*