AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011255

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011255 MtrunA17_Chr3g0082861 97.006 167 5 0 1 167 1 167 4.56e-118 330
MsaG011255 MtrunA17_Chr8g0352871 81.757 148 26 1 3 150 8 154 1.24e-84 246
MsaG011255 MtrunA17_Chr7g0256551 72.297 148 41 0 2 149 7 154 2.04e-77 230
MsaG011255 MtrunA17_Chr3g0124861 58.491 159 52 3 3 150 21 176 1.06e-58 182
MsaG011255 MtrunA17_Chr5g0414931 56.643 143 57 1 3 145 15 152 4.94e-53 168
MsaG011255 MtrunA17_Chr1g0170741 54.140 157 63 2 3 159 7 154 1.67e-51 163
MsaG011255 MtrunA17_Chr4g0059571 57.534 146 53 2 2 147 24 160 1.88e-51 163
MsaG011255 MtrunA17_Chr3g0118211 51.923 156 66 2 1 156 20 166 8.53e-51 161
MsaG011255 MtrunA17_Chr3g0128691 55.634 142 46 3 5 141 19 148 3.54e-50 158
MsaG011255 MtrunA17_Chr4g0021731 73.864 88 14 1 3 90 10 88 9.25e-41 132
MsaG011255 MtrunA17_Chr4g0030271 29.927 137 78 2 6 142 91 209 1.91e-14 70.1
MsaG011255 MtrunA17_Chr8g0345901 27.143 140 84 1 6 145 38 159 1.95e-14 70.1
MsaG011255 MtrunA17_Chr3g0127941 27.333 150 91 2 4 153 56 187 9.18e-13 65.1
MsaG011255 MtrunA17_Chr1g0181811 27.737 137 81 1 6 142 87 205 2.23e-12 63.9
MsaG011255 MtrunA17_Chr7g0276361 25.166 151 93 2 1 151 13 143 2.36e-12 63.9
MsaG011255 MtrunA17_Chr7g0275401 29.221 154 85 4 4 154 9 141 1.12e-11 61.6
MsaG011255 MtrunA17_Chr1g0149851 28.671 143 81 3 6 148 11 132 6.59e-11 59.7
MsaG011255 MtrunA17_Chr7g0275891 28.777 139 79 3 6 144 11 129 6.74e-11 59.7
MsaG011255 MtrunA17_Chr8g0376881 26.207 145 86 3 2 146 27 150 9.93e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011255 AT3G57040.2 69.799 149 42 2 2 148 7 154 3.56e-71 214
MsaG011255 AT3G57040.1 69.799 149 42 2 2 148 7 154 3.56e-71 214
MsaG011255 AT2G41310.1 64.430 149 52 1 2 150 7 154 4.70e-67 204
MsaG011255 AT1G59940.1 57.962 157 52 4 3 158 32 175 2.81e-56 177
MsaG011255 AT1G59940.2 57.962 157 52 4 3 158 124 267 1.72e-54 175
MsaG011255 AT1G10470.3 56.604 159 54 5 1 158 17 161 3.09e-54 172
MsaG011255 AT1G10470.1 56.604 159 54 5 1 158 31 175 5.45e-54 172
MsaG011255 AT3G56380.1 55.634 142 55 2 3 144 19 152 2.41e-52 164
MsaG011255 AT3G48100.1 52.500 160 67 3 4 163 25 175 3.70e-52 164
MsaG011255 AT2G40670.1 53.521 142 60 1 3 144 28 163 2.59e-51 162
MsaG011255 AT5G62920.1 57.447 141 51 2 4 144 25 156 1.17e-50 161
MsaG011255 AT3G56380.2 55.245 143 55 3 3 144 19 153 1.68e-50 159
MsaG011255 AT2G40670.2 53.147 143 60 2 3 144 28 164 1.80e-49 157
MsaG011255 AT1G19050.1 50.000 158 69 2 3 159 23 171 3.98e-49 157
MsaG011255 AT1G74890.1 54.286 140 55 1 3 142 17 147 2.85e-48 155
MsaG011255 AT2G07440.1 66.667 60 20 0 91 150 9 68 4.45e-22 86.7
MsaG011255 AT1G10470.2 52.857 70 27 2 90 158 35 99 2.31e-16 73.2
MsaG011255 AT5G24470.1 28.777 139 79 3 6 144 54 172 3.74e-13 66.6
MsaG011255 AT5G60100.2 28.467 137 80 1 6 142 66 184 9.06e-12 62.8
MsaG011255 AT5G60100.7 28.467 137 80 1 6 142 66 184 9.49e-12 62.4
MsaG011255 AT5G60100.6 28.467 137 80 1 6 142 66 184 9.49e-12 62.4
MsaG011255 AT5G60100.4 28.467 137 80 1 6 142 66 184 9.49e-12 62.4
MsaG011255 AT5G60100.5 28.467 137 80 1 6 142 66 184 1.78e-11 61.6
MsaG011255 AT5G60100.3 28.467 137 80 1 6 142 66 184 1.78e-11 61.6
MsaG011255 AT5G60100.1 28.467 137 80 1 6 142 66 184 1.78e-11 61.6
MsaG011255 AT5G07210.2 30.000 140 76 3 5 144 17 134 2.52e-11 61.2
MsaG011255 AT2G25180.1 27.451 153 89 4 6 157 19 150 2.75e-11 61.2
MsaG011255 AT2G46790.3 26.619 139 84 2 4 142 37 157 2.76e-11 61.2
MsaG011255 AT2G46790.1 26.619 139 84 2 4 142 37 157 3.15e-11 61.2
MsaG011255 AT5G02810.1 26.277 137 83 2 6 142 80 198 5.91e-11 60.5

Find 24 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGCACAGTTTCATATTT+TGG 0.112277 3:+99323 None:intergenic
TCAGTTACTGTGGTTGATTC+TGG 0.242120 3:+99873 MsaT011255.1:intron
CTCAAAACCTCTTCATTTCA+TGG 0.264136 3:+99390 MsaT011255.1:CDS
GGCTTTGAAGTTTCTTGGTT+TGG 0.274386 3:+99902 MsaT011255.1:CDS
ACTAAGGCTTTGAAGTTTCT+TGG 0.296459 3:+99897 MsaT011255.1:CDS
GGTTACCTGTTAATCCTTGA+TGG 0.331015 3:-100775 None:intergenic
TGTGGTTGATTCTGGTACTA+AGG 0.342411 3:+99881 MsaT011255.1:CDS
TTAACATCTGATTGTTGAAC+TGG 0.359149 3:-100946 None:intergenic
AGATCATAGCCTGTCATTCC+TGG 0.369947 3:-100138 None:intergenic
ATGTTCCATCAAGGATTAAC+AGG 0.384039 3:+100770 MsaT011255.1:CDS
GTTTCTGCAGCAATAGTTTG+AGG 0.420290 3:-99951 None:intergenic
TACTGTATGCCAGGAATGAC+AGG 0.454246 3:+100129 MsaT011255.1:CDS
TGAATCAGATGCTTAGAAGA+AGG 0.516221 3:+100901 MsaT011255.1:intron
ATAACTGATTACTGTATGCC+AGG 0.518630 3:+100120 MsaT011255.1:CDS
ACCTAATCAACAACAAAAGA+AGG 0.519626 3:+101016 MsaT011255.1:CDS
TTGACAGAATGTTGATTGAA+AGG 0.523765 3:+99364 MsaT011255.1:CDS
AAAATTACCATGAAATGAAG+AGG 0.534519 3:-99397 None:intergenic
TATTGCTGCAGAAACAGATC+AGG 0.546049 3:+99959 MsaT011255.1:CDS
TCTTGGTTTGGTTGAAGATG+AGG 0.555035 3:+99914 MsaT011255.1:CDS
CCTCAGAGAATGTTCCATCA+AGG 0.587746 3:+100761 MsaT011255.1:CDS
TCTGAGGACATAATCACCAC+TGG 0.603381 3:-100745 None:intergenic
GTTTGGTTGAAGATGAGGTG+AGG 0.669904 3:+99919 MsaT011255.1:CDS
TTCTCTTAAAGACATACCAG+TGG 0.689344 3:+100729 MsaT011255.1:CDS
CCTTGATGGAACATTCTCTG+AGG 0.703773 3:-100761 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 99324 101071 99324 ID=MsaG011255
Chr3 mRNA 99324 101071 99324 ID=MsaT011255.1;Parent=MsaG011255
Chr3 exon 99324 99411 99324 ID=MsaT011255.1.exon1;Parent=MsaT011255.1
Chr3 CDS 99324 99411 99324 ID=cds.MsaT011255.1;Parent=MsaT011255.1
Chr3 exon 99877 99980 99877 ID=MsaT011255.1.exon2;Parent=MsaT011255.1
Chr3 CDS 99877 99980 99877 ID=cds.MsaT011255.1;Parent=MsaT011255.1
Chr3 exon 100099 100176 100099 ID=MsaT011255.1.exon3;Parent=MsaT011255.1
Chr3 CDS 100099 100176 100099 ID=cds.MsaT011255.1;Parent=MsaT011255.1
Chr3 exon 100721 100791 100721 ID=MsaT011255.1.exon4;Parent=MsaT011255.1
Chr3 CDS 100721 100791 100721 ID=cds.MsaT011255.1;Parent=MsaT011255.1
Chr3 exon 100909 101071 100909 ID=MsaT011255.1.exon5;Parent=MsaT011255.1
Chr3 CDS 100909 101071 100909 ID=cds.MsaT011255.1;Parent=MsaT011255.1
Gene Sequence

>MsaT011255.1

ATGGCACAGTTTCATATTTTGGCTGTTGATGACAGTGTTATTGACAGAATGTTGATTGAAAGGCTGCTCAAAACCTCTTCATTTCATGTTACTGTGGTTGATTCTGGTACTAAGGCTTTGAAGTTTCTTGGTTTGGTTGAAGATGAGGTGAGGAATGAAAAGCCTCAAACTATTGCTGCAGAAACAGATCAGGTTGTAGAAGTGAATCTGATCATAACTGATTACTGTATGCCAGGAATGACAGGCTATGATCTGCTGAGAAAAATCAAGGAATCTAATTCTCTTAAAGACATACCAGTGGTGATTATGTCCTCAGAGAATGTTCCATCAAGGATTAACAGATGCTTAGAAGAAGGAGCTGAAGAATTCTTTCTGAAACCAGTTCAACAATCAGATGTTAACAAGTTGAAACCACATTTGTTGAAGACAAAAGTTAAGAATGAAGATGACCTAATCAACAACAAAAGAAGGATACAAATCAATGCAATTCCATTGAAAAACTAG
Protein sequence

>MsaT011255.1

MAQFHILAVDDSVIDRMLIERLLKTSSFHVTVVDSGTKALKFLGLVEDEVRNEKPQTIAAETDQVVEVNLIITDYCMPGMTGYDLLRKIKESNSLKDIPVVIMSSENVPSRINRCLEEGAEEFFLKPVQQSDVNKLKPHLLKTKVKNEDDLINNKRRIQINAIPLKN*