AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011278

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011278 MtrunA17_Chr3g0083491 94.144 1332 55 4 1 1310 1 1331 0.0 2550
MsaG011278 MtrunA17_Chr2g0320751 25.578 606 323 21 707 1273 476 992 1.76e-38 156
MsaG011278 MtrunA17_Chr7g0255821 23.864 616 351 20 707 1296 294 817 1.97e-34 142
MsaG011278 MtrunA17_Chr1g0168031 25.331 604 334 17 706 1263 588 1120 6.50e-33 139
MsaG011278 MtrunA17_Chr2g0281601 22.222 639 391 20 707 1268 661 1270 2.43e-22 104
MsaG011278 MtrunA17_Chr4g0024921 22.449 539 289 18 774 1266 447 902 6.61e-21 99.8
MsaG011278 MtrunA17_Chr1g0212331 28.488 172 112 4 1127 1297 871 1032 3.90e-16 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011278 AT3G54460.1 52.899 1397 515 20 12 1310 5 1356 0.0 1408
MsaG011278 AT3G54460.2 57.260 1095 401 11 241 1310 7 1059 0.0 1240
MsaG011278 AT5G22750.1 25.950 605 312 20 712 1273 494 1005 1.40e-41 167
MsaG011278 AT1G11100.1 25.238 630 399 22 709 1305 634 1224 3.81e-32 137
MsaG011278 AT1G11100.5 25.238 630 399 22 709 1305 491 1081 3.87e-32 136
MsaG011278 AT1G11100.3 24.036 674 396 23 709 1305 491 1125 2.97e-29 127
MsaG011278 AT1G11100.2 24.036 674 396 23 709 1305 633 1267 3.47e-29 127
MsaG011278 AT1G11100.4 24.036 674 396 23 709 1305 634 1268 3.88e-29 127
MsaG011278 AT3G20010.2 22.624 663 401 20 709 1297 124 748 3.63e-26 116
MsaG011278 AT3G20010.3 22.624 663 401 20 709 1297 149 773 5.06e-26 116
MsaG011278 AT3G20010.1 22.072 666 393 19 709 1293 415 1035 1.05e-25 115
MsaG011278 AT3G20010.5 22.072 666 393 19 709 1293 415 1035 1.05e-25 115
MsaG011278 AT3G20010.7 22.072 666 393 19 709 1293 415 1035 1.05e-25 115
MsaG011278 AT1G61140.3 22.576 660 390 21 709 1299 508 1115 5.17e-25 113
MsaG011278 AT1G61140.1 22.576 660 390 21 709 1299 666 1273 5.39e-25 114
MsaG011278 AT1G50410.2 24.760 521 333 18 777 1272 466 952 2.75e-24 111
MsaG011278 AT1G50410.1 24.760 521 333 18 777 1272 466 952 2.75e-24 111
MsaG011278 AT1G50410.3 24.760 521 333 18 777 1272 466 952 2.75e-24 111
MsaG011278 AT5G05130.1 29.545 220 137 8 700 913 349 556 2.74e-19 94.7
MsaG011278 AT5G43530.1 25.575 348 204 12 709 1026 742 1064 3.90e-17 88.2
MsaG011278 AT5G43530.1 30.657 137 94 1 1128 1263 1108 1244 8.81e-14 77.0
MsaG011278 AT1G02670.6 32.479 117 78 1 1149 1264 512 628 6.01e-15 80.5
MsaG011278 AT1G02670.3 32.479 117 78 1 1149 1264 539 655 6.09e-15 80.5
MsaG011278 AT1G02670.1 32.479 117 78 1 1149 1264 527 643 1.16e-14 79.7
MsaG011278 AT1G02670.7 32.479 117 78 1 1149 1264 516 632 1.19e-14 79.3
MsaG011278 AT1G05120.1 31.092 119 81 1 1149 1266 682 800 2.06e-14 79.0
MsaG011278 AT1G05120.2 31.092 119 81 1 1149 1266 725 843 2.20e-14 79.0
MsaG011278 AT1G02670.5 31.068 103 70 1 1149 1250 512 614 1.31e-11 69.7
MsaG011278 AT1G02670.2 31.068 103 70 1 1149 1250 539 641 1.32e-11 69.7

Find 292 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTTGTCAATAGAAATTT+TGG 0.175597 3:+745592 None:intergenic
GGCTGGTGGTTATACTATTA+TGG 0.190268 3:-746311 MsaT011278.1:CDS
CCGAAATCTGGTTCTGTTGC+TGG 0.192784 3:-748845 MsaT011278.1:CDS
CATTGCGTTGAATCAATAAT+TGG 0.217099 3:+749082 None:intergenic
CTACGAAATTTCCACTGTTT+TGG 0.219815 3:+744645 None:intergenic
TGTTGAATGAACAGGATAAT+TGG 0.223532 3:-744041 MsaT011278.1:intron
AGTAGTTGTTCCAGCAAATT+TGG 0.223945 3:-745324 MsaT011278.1:CDS
CTGATCAGGATTTAACAATT+TGG 0.226507 3:+746222 None:intergenic
TGATGTTTATCTTCCGGTTT+TGG 0.272070 3:-748891 MsaT011278.1:CDS
TGGAGGTTGATTCTAGGGTT+AGG 0.272143 3:-748928 MsaT011278.1:CDS
TAATGCAAGTGCACTGGTTT+AGG 0.287123 3:-745127 MsaT011278.1:CDS
CATCTGCGCACTCGCTTGCC+TGG 0.292942 3:-745220 MsaT011278.1:CDS
AAGTTGTATGGCACTTCTAA+TGG 0.293170 3:-743423 MsaT011278.1:CDS
CAGCAAATTTGGTTGATCAT+TGG 0.295875 3:-745313 MsaT011278.1:CDS
CCAGAGGTTGAAAGCTTTGC+AGG 0.299028 3:-743971 MsaT011278.1:CDS
CAATGATCAACCAAATTTGC+TGG 0.299556 3:+745314 None:intergenic
ATACTGGCGGATCTGGGATT+TGG 0.301244 3:-746810 MsaT011278.1:CDS
GTTGATACTTCATATGGTTC+TGG 0.303091 3:-745959 MsaT011278.1:CDS
GTGTTGTGGAGGTTGATTCT+AGG 0.304442 3:-748934 MsaT011278.1:CDS
TTCCTTCATGAAGAAGCTTA+TGG 0.308034 3:-744942 MsaT011278.1:CDS
CACAAAGTATTTCAAGCATT+TGG 0.310911 3:-745491 MsaT011278.1:CDS
ATTTACCTTCTGTCCCAAAT+TGG 0.311155 3:+743341 None:intergenic
TCTGGCCGTGCCATTGAATC+AGG 0.311287 3:+744212 None:intergenic
TGGGCCCACTGGACCTCAAT+AGG 0.312568 3:-746747 MsaT011278.1:CDS
AATGGTCTAGATTCTACTTC+AGG 0.317795 3:-744519 MsaT011278.1:CDS
GTCAATTGCATGTTTATGTT+TGG 0.319332 3:-745259 MsaT011278.1:CDS
TATTGACAAGCTTGCAGAAA+TGG 0.319995 3:-745582 MsaT011278.1:CDS
AGGCTTTCATGCTGTGTTGC+TGG 0.327727 3:-744603 MsaT011278.1:CDS
CTAGATGTTGATTGCCAATC+AGG 0.333695 3:+744015 None:intergenic
ATGGACAATACTGGCGGATC+TGG 0.335524 3:-746817 MsaT011278.1:CDS
AAAGTGCAGTTGTTTAAAAT+AGG 0.342173 3:+745539 None:intergenic
TTGGAGAAAATGAGAGCATT+TGG 0.344489 3:-746992 MsaT011278.1:CDS
TACTGGCGGATCTGGGATTT+GGG 0.346850 3:-746809 MsaT011278.1:CDS
GGTTGGCAGTTTCCGAAATC+TGG 0.347321 3:-748857 MsaT011278.1:CDS
AGGGATGTGTGCCAAGATTC+AGG 0.351546 3:-746283 MsaT011278.1:CDS
CTTTCTGATTGTCATGTACT+TGG 0.351744 3:-746966 MsaT011278.1:CDS
GGAGGTTGATTCTAGGGTTA+GGG 0.353484 3:-748927 MsaT011278.1:CDS
TTCCATGTAAATACTGTAAC+TGG 0.361846 3:-746547 MsaT011278.1:CDS
CATTACTTCTACCTGAATCT+TGG 0.363612 3:+746272 None:intergenic
GTTAATGGTATCAAATATAC+TGG 0.364522 3:-743637 MsaT011278.1:CDS
TCTCTGTGAGCCATTAGATT+TGG 0.364555 3:-745375 MsaT011278.1:CDS
AAAGCTTGCGGATAACAGTA+TGG 0.366539 3:-745846 MsaT011278.1:CDS
TAATGCTACTTGAACATTCC+TGG 0.371689 3:+743851 None:intergenic
TGCTGAAAGCAATTATCTAC+TGG 0.373523 3:-742663 MsaT011278.1:CDS
GTTGATAGAGCTGCATATAA+AGG 0.376494 3:+746586 None:intergenic
GCGAAGAAGGTGAAGAAGGT+TGG 0.378619 3:-749043 MsaT011278.1:CDS
GTTGCAAATGACTATTTCAT+TGG 0.380919 3:-745051 MsaT011278.1:CDS
CTTGGGGTACAGACCACTTT+GGG 0.382124 3:+744180 None:intergenic
GAAGCGTAAAGTTGATTGTT+TGG 0.386718 3:-745915 MsaT011278.1:CDS
GCTAATGTTTCCACATGAAT+AGG 0.389546 3:+743000 None:intergenic
TTGTAACTCAATAAGATCTT+GGG 0.390486 3:+744163 None:intergenic
TCAAATCGGTGGATACTAAC+TGG 0.393580 3:-745023 MsaT011278.1:CDS
AAACAGGAGACTGTATCTTA+TGG 0.393932 3:+744485 None:intergenic
AATTAAAATTCTATATTCCC+TGG 0.394953 3:-746767 MsaT011278.1:CDS
AAGAAGGTTGGGATGGTGAA+TGG 0.398183 3:-749031 MsaT011278.1:CDS
GTTGTAACTCAATAAGATCT+TGG 0.398290 3:+744162 None:intergenic
TCCACGAAAATCTTTGATAG+TGG 0.398654 3:+746501 None:intergenic
AAGCTTGTTGATACTTCATA+TGG 0.400306 3:-745965 MsaT011278.1:CDS
TCGTTGTTGGTGGTGGTCAT+TGG 0.401757 3:+749259 None:intergenic
ATTGTCCATAGGTATTATCT+TGG 0.402393 3:+746831 None:intergenic
GTTGAATGAACAGGATAATT+GGG 0.403727 3:-744040 MsaT011278.1:intron
GTGAGCCATTAGATTTGGTT+AGG 0.403839 3:-745370 MsaT011278.1:CDS
TGTTTGGTGGCCACTGTGTC+AGG 0.403966 3:-744467 MsaT011278.1:intron
CCTGCAAAGCTTTCAACCTC+TGG 0.409697 3:+743971 None:intergenic
GTTATACTATTATGGGTAAG+AGG 0.409884 3:-746303 MsaT011278.1:CDS
AGAGCTTATGCAGCTGTGAT+TGG 0.410613 3:-747064 MsaT011278.1:intron
CCTTGTTGCTAGAATGCATT+GGG 0.411990 3:+743605 None:intergenic
GGTACAGACCACTTTGGGTT+AGG 0.415400 3:+744185 None:intergenic
ACAACAATACAGTCGCGTTT+CGG 0.418999 3:-749129 MsaT011278.1:CDS
CAGAGTTTGTGGGTTGAGAA+AGG 0.420980 3:+749235 None:intergenic
CCAGCAACAGAACCAGATTT+CGG 0.421076 3:+748845 None:intergenic
AGATGTGTAAGTTGGCTGTT+AGG 0.423057 3:+744981 None:intergenic
CTGTTCTTAAAAGACTGTTC+TGG 0.424653 3:+745758 None:intergenic
CCAGCACGATTCAAGTTGTA+TGG 0.427164 3:-743435 MsaT011278.1:CDS
GCTGGTGGTTATACTATTAT+GGG 0.427840 3:-746310 MsaT011278.1:CDS
GGGCCCACTGGACCTCAATA+GGG 0.428655 3:-746746 MsaT011278.1:CDS
TGAGAAAGGGTCGTTGTTGG+TGG 0.429083 3:+749249 None:intergenic
ACAACTGCACTTTGTCCAAC+GGG 0.430181 3:-745529 MsaT011278.1:CDS
TCATTCCGCATCATCATTCC+AGG 0.431472 3:-743869 MsaT011278.1:CDS
AGAGAAAACCACAGAGTTTG+TGG 0.431603 3:+749224 None:intergenic
ACTTGAATCGTGCTGGAACT+TGG 0.432061 3:+743442 None:intergenic
AAGTAGAGAAAGGTGTATAC+AGG 0.433050 3:-745457 MsaT011278.1:CDS
TATACATGTCATTGAACAAC+AGG 0.433121 3:-743776 MsaT011278.1:intron
CTCTTTCAGAGGAGAAATCA+TGG 0.434595 3:+746245 None:intergenic
AGTATCTTTCTACAGTAGTC+TGG 0.436177 3:+747014 None:intergenic
CGAAGAAGGTGAAGAAGGTT+GGG 0.440804 3:-749042 MsaT011278.1:CDS
TATAACCTAACCAAATCTAA+TGG 0.441840 3:+745365 None:intergenic
TCTATATTCCCTGGGCCCAC+TGG 0.443461 3:-746758 MsaT011278.1:CDS
CAAATTGTTAAATCCTGATC+AGG 0.446046 3:-746221 MsaT011278.1:CDS
TAGCCGTTTGAGTGCGGAGT+GGG 0.446474 3:-745171 MsaT011278.1:CDS
TATTTCCAAGATAATACCTA+TGG 0.446650 3:-746836 MsaT011278.1:CDS
TGTACACTTTGTCTTGTCAA+TGG 0.446929 3:+744271 None:intergenic
AACATAAACATGCAATTGAC+CGG 0.447447 3:+745262 None:intergenic
TTTGCCAACTCTTCTTTCGT+TGG 0.447961 3:+746042 None:intergenic
CCACTACTCCAAACCAAAAC+CGG 0.449559 3:+748878 None:intergenic
AGGATAATTGGGATCCTGAT+TGG 0.449928 3:-744029 MsaT011278.1:CDS
ATAATACCTATGGACAATAC+TGG 0.450114 3:-746826 MsaT011278.1:CDS
TGTTGTGGAGGTTGATTCTA+GGG 0.450644 3:-748933 MsaT011278.1:CDS
TTTCATTGGTTGCTTCAAAT+CGG 0.451100 3:-745037 MsaT011278.1:CDS
GCTTGAAATACTTTGTGGTA+TGG 0.451295 3:+745497 None:intergenic
TGATGTGGCTGCCCTTGGTA+TGG 0.453648 3:-745399 MsaT011278.1:CDS
ATCTGCGCACTCGCTTGCCT+GGG 0.456519 3:-745219 MsaT011278.1:CDS
CACAAATAGAAAAGCATGTC+GGG 0.456638 3:-745286 MsaT011278.1:CDS
CAGATGGGTCACCAGTAGTC+TGG 0.456981 3:-746390 MsaT011278.1:CDS
ATAAGATACAGTCTCCTGTT+TGG 0.458193 3:-744483 MsaT011278.1:CDS
GCAACTTGTTTGTTAAGCTC+AGG 0.460038 3:+745069 None:intergenic
GGTTGAGAAAGGGTCGTTGT+TGG 0.461620 3:+749246 None:intergenic
TTAGATGAAGGGCATACTCT+TGG 0.462268 3:-745098 MsaT011278.1:CDS
AATGGATGGGAGTGCTGCAT+TGG 0.465372 3:-743405 MsaT011278.1:CDS
CATGGGATGCTGGCGTGCTT+AGG 0.467040 3:-744905 MsaT011278.1:CDS
CAAATGCTTGAAATACTTTG+TGG 0.470118 3:+745492 None:intergenic
GGTTTAGTGATTTCCTGATC+AGG 0.470225 3:+746208 None:intergenic
TGTTCGGCGTAATATATTGA+TGG 0.471628 3:-744709 MsaT011278.1:CDS
ATTAAAATTCTATATTCCCT+GGG 0.473601 3:-746766 MsaT011278.1:CDS
GATTGTTGATGTTTATCTTC+CGG 0.475214 3:-748897 MsaT011278.1:CDS
AGAAGGTTGGGATGGTGAAT+GGG 0.475682 3:-749030 MsaT011278.1:CDS
CACCAGTTACAGTATTTACA+TGG 0.476766 3:+746545 None:intergenic
CGAGAGTTCGAGTGCGAAGA+AGG 0.476982 3:-749056 MsaT011278.1:CDS
ATCAGCAGGCAGCAGTTGAA+TGG 0.479240 3:-746648 MsaT011278.1:CDS
CTTGCAGAAATGGAAACAAA+TGG 0.479957 3:-745572 MsaT011278.1:CDS
TAACTGGTGATATAGTCATC+GGG 0.482511 3:-746531 MsaT011278.1:CDS
TATTCCTCAGAGCAAGCATC+AGG 0.483783 3:+746148 None:intergenic
TGACACAGTGGCCACCAAAC+AGG 0.484233 3:+744469 None:intergenic
GTCCATAAGCTTCTTCATGA+AGG 0.485696 3:+744940 None:intergenic
TATTGAGGTCCAGTGGGCCC+AGG 0.485759 3:+746749 None:intergenic
ATGCATGATTAGTGCTAGAA+AGG 0.486577 3:-744826 MsaT011278.1:CDS
ATGGTCTAGATTCTACTTCA+GGG 0.489153 3:-744518 MsaT011278.1:CDS
ATGGATGGGAGTGCTGCATT+GGG 0.489759 3:-743404 MsaT011278.1:CDS
ACACGCTGGAGAAGATATTC+AGG 0.491050 3:-744565 MsaT011278.1:CDS
AATTAGTCGTGCCCATCGTA+TGG 0.491970 3:-743034 MsaT011278.1:CDS
TGGCGTGCTTAGGCCATTCG+AGG 0.492502 3:-744895 MsaT011278.1:CDS
CAGGCAAGCGAGTGCGCAGA+TGG 0.495564 3:+745221 None:intergenic
CAGACTACTGGTGACCCATC+TGG 0.497077 3:+746391 None:intergenic
TTACCAGGATTTCACTTGAA+AGG 0.498175 3:-745719 MsaT011278.1:CDS
GATGCAAAGTCCTGATTCAA+TGG 0.499559 3:-744222 MsaT011278.1:CDS
TTTATGTTTGGAACGATCAT+CGG 0.500097 3:-745247 MsaT011278.1:CDS
CTGTTAGGAGTGTTCGATGT+TGG 0.500631 3:+744996 None:intergenic
AAATCTGTTTCCTCATCAGC+AGG 0.501336 3:-746662 MsaT011278.1:CDS
TCAACTGCTGCCTGCTGATG+AGG 0.502624 3:+746652 None:intergenic
ACTCTACAACTCTGATACAA+AGG 0.503506 3:+745782 None:intergenic
ACCTTGTTGCTAGAATGCAT+TGG 0.503718 3:+743604 None:intergenic
GAAAGGGTCGTTGTTGGTGG+TGG 0.505667 3:+749252 None:intergenic
AACAGATTTAACTTTGTGCA+AGG 0.507122 3:+746676 None:intergenic
CTTCTAGTGCACCGACTCGC+AGG 0.507703 3:+746121 None:intergenic
ACTACTCATTGGTAAATTCT+CGG 0.510262 3:-745640 MsaT011278.1:CDS
GTAATATATTGATGGCTGAT+TGG 0.511643 3:-744701 MsaT011278.1:CDS
GGCCCCACTCCGCACTCAAA+CGG 0.512630 3:+745168 None:intergenic
ATAGAAAAGCATGTCGGGCC+CGG 0.512983 3:-745281 MsaT011278.1:CDS
TGAAGAACCGCTCACAGGAA+TGG 0.515280 3:+746934 None:intergenic
GTTCCTTTCAAGTGAAATCC+TGG 0.515719 3:+745716 None:intergenic
GCTTAAGGAGCACTACTCAT+TGG 0.516177 3:-745651 MsaT011278.1:CDS
TGTATGGCACTTCTAATGGA+TGG 0.516417 3:-743419 MsaT011278.1:CDS
TGGAGCCAATTTGGGACAGA+AGG 0.516927 3:-743346 MsaT011278.1:intron
GTCGCGTTTCGGACTCAAAA+CGG 0.517298 3:-749118 MsaT011278.1:CDS
TCTTCCATCTCTGCCTCGAA+TGG 0.518095 3:+744882 None:intergenic
AGAGTTTGTGGGTTGAGAAA+GGG 0.518710 3:+749236 None:intergenic
CCATACAACTTGAATCGTGC+TGG 0.522662 3:+743435 None:intergenic
AACAACTGCACTTTGTCCAA+CGG 0.522924 3:-745530 MsaT011278.1:CDS
GACTACTGTAGAAAGATACT+TGG 0.523086 3:-747011 MsaT011278.1:CDS
GTCGACATGGCGTAATGAAA+GGG 0.524194 3:+744318 None:intergenic
AGCCATGCCTGCGCATAAGA+AGG 0.527424 3:+744140 None:intergenic
TCTTGGGGTACAGACCACTT+TGG 0.527858 3:+744179 None:intergenic
TGGACAATACTGGCGGATCT+GGG 0.528838 3:-746816 MsaT011278.1:CDS
AATGTGATGCTTGTCACAAA+TGG 0.532521 3:-745871 MsaT011278.1:CDS
CTCTGCTGATTTCCTACACA+AGG 0.532791 3:+746865 None:intergenic
AAAACAACTTTCTTGATGCA+TGG 0.533458 3:+744783 None:intergenic
AAGACAAAGTGTACATATCC+TGG 0.533923 3:-744263 MsaT011278.1:CDS
CACAAAGACGCAAGGTACAT+TGG 0.534529 3:-746422 MsaT011278.1:CDS
TGTCGACATGGCGTAATGAA+AGG 0.537636 3:+744317 None:intergenic
CACATCTACAACCATTGCTA+AGG 0.537808 3:-744965 MsaT011278.1:CDS
ATTTCCAACGAAAGAAGAGT+TGG 0.540007 3:-746046 MsaT011278.1:CDS
ATGAGTTGGTACTCACTGTT+CGG 0.540678 3:-744725 MsaT011278.1:CDS
AGGAATTACTGAAGCAGCCA+AGG 0.540726 3:+746696 None:intergenic
CCTCATGATTCATGTTCTGT+AGG 0.541036 3:-746187 MsaT011278.1:CDS
CATTGGTTGCTTCAAATCGG+TGG 0.542676 3:-745034 MsaT011278.1:CDS
AGGAATATTCGCCTGCGAGT+CGG 0.543870 3:-746132 MsaT011278.1:CDS
GTTACAACCTTCTTATGCGC+AGG 0.544047 3:-744147 MsaT011278.1:intron
ATCAGTGGTAATAACACGGC+TGG 0.544193 3:-746328 MsaT011278.1:CDS
GTTACAGTCTTACCCAAGCC+AGG 0.544684 3:+746460 None:intergenic
CTCTACAACTCTGATACAAA+GGG 0.545584 3:+745783 None:intergenic
GAGAAAACCACAGAGTTTGT+GGG 0.545584 3:+749225 None:intergenic
GCAGCTCTATCAACTGATGA+TGG 0.546385 3:-746577 MsaT011278.1:CDS
ATTGGTAAATTCTCGGACAA+AGG 0.547067 3:-745633 MsaT011278.1:CDS
ATTTCACTTGAAAGGAACTC+CGG 0.547183 3:-745711 MsaT011278.1:CDS
GACAGAGTTACAAGTGGCAT+GGG 0.550843 3:-745937 MsaT011278.1:CDS
TTCCTACACAAGGGAAGGTA+CGG 0.554989 3:+746875 None:intergenic
TAGGCCATTCGAGGCAGAGA+TGG 0.555199 3:-744886 MsaT011278.1:CDS
CCCAATGCATTCTAGCAACA+AGG 0.557176 3:-743605 MsaT011278.1:intron
ATTGAGGTCCAGTGGGCCCA+GGG 0.559176 3:+746750 None:intergenic
GGACAAAGGAGGCTCTGACT+TGG 0.560926 3:-745619 MsaT011278.1:CDS
GAGTGCGAAGAAGGTGAAGA+AGG 0.561114 3:-749047 MsaT011278.1:CDS
TCTAAGATAACTTACTTACC+AGG 0.562932 3:-745734 MsaT011278.1:CDS
TACATTTAGCCGTTTGAGTG+CGG 0.563559 3:-745177 MsaT011278.1:CDS
CCACGAAAATCTTTGATAGT+GGG 0.564131 3:+746502 None:intergenic
GGACAGAGTTACAAGTGGCA+TGG 0.565878 3:-745938 MsaT011278.1:CDS
GAAGGTGAAGAAGGTTGGGA+TGG 0.566498 3:-749038 MsaT011278.1:CDS
GTGCTCTAATGCAAGTGCAC+TGG 0.567512 3:-745133 MsaT011278.1:CDS
CGGAGGGGTTAGTGTGAGAA+CGG 0.567994 3:+749198 None:intergenic
ATTAGAGCACTCTTCTTACG+TGG 0.568097 3:+745146 None:intergenic
GTAACTGGTGATATAGTCAT+CGG 0.568117 3:-746532 MsaT011278.1:CDS
AGATACAGTCTCCTGTTTGG+TGG 0.570067 3:-744480 MsaT011278.1:CDS
AGAGCTGCATATAAAGGATG+TGG 0.570630 3:+746592 None:intergenic
GATGACAACATCATAATCCC+AGG 0.571751 3:+745202 None:intergenic
TTATGAATTTCGTAGGTGCA+AGG 0.572108 3:-744354 MsaT011278.1:intron
TTATACTATTATGGGTAAGA+GGG 0.573699 3:-746302 MsaT011278.1:CDS
ATGTCATCATCTAAGAGCCT+TGG 0.574388 3:-746713 MsaT011278.1:CDS
TGCGTCCCGTCCTATTCATG+TGG 0.574636 3:-743010 MsaT011278.1:CDS
TAACAATTTGGCTCTTTCAG+AGG 0.574738 3:+746234 None:intergenic
TTCGAGGCAGAGATGGAAGA+AGG 0.574892 3:-744879 MsaT011278.1:CDS
TCACAAATGGCGAAAGCTTG+CGG 0.575828 3:-745858 MsaT011278.1:CDS
CAGAAAATCCTAACCCAAAG+TGG 0.576600 3:-744193 MsaT011278.1:CDS
AAAGGAGGCTCTGACTTGGT+TGG 0.577350 3:-745615 MsaT011278.1:CDS
TTTCACTTGAAAGGAACTCC+GGG 0.578633 3:-745710 MsaT011278.1:CDS
GTATGGCACTTCTAATGGAT+GGG 0.579018 3:-743418 MsaT011278.1:CDS
TCATACAAGTTATCACAGCC+AGG 0.581057 3:+744245 None:intergenic
TTATGTTGACACCAGACTAC+TGG 0.582238 3:+746379 None:intergenic
TGTGAATCAGCTTCATGCGT+TGG 0.583418 3:-748996 MsaT011278.1:CDS
ATTAGTCGTGCCCATCGTAT+GGG 0.583520 3:-743033 MsaT011278.1:CDS
AGAAGATATTCAGGAAACAA+TGG 0.583877 3:-744556 MsaT011278.1:CDS
TGTCAATGGAAACACAATCA+AGG 0.590521 3:+744285 None:intergenic
ACATTGGCGGATCCACCAGA+TGG 0.590534 3:-746406 MsaT011278.1:CDS
GATGTTGTCAAATCTGAAGA+TGG 0.590681 3:-742719 MsaT011278.1:CDS
GACGGGACGCACCCATACGA+TGG 0.591504 3:+743022 None:intergenic
TGTTTCCACATGAATAGGAC+GGG 0.593054 3:+743005 None:intergenic
TCATGAAGGAACCTTAGCAA+TGG 0.594391 3:+744954 None:intergenic
TTGTCAAATCTGAAGATGGT+GGG 0.595354 3:-742715 MsaT011278.1:CDS
CTGATTTCCTACACAAGGGA+AGG 0.600449 3:+746870 None:intergenic
TGCTAGAAGTTACAATGAGT+TGG 0.601484 3:-744739 MsaT011278.1:CDS
CAGAGGTTGAAAGCTTTGCA+GGG 0.604660 3:-743970 MsaT011278.1:CDS
AGCCGTTTGAGTGCGGAGTG+GGG 0.605704 3:-745170 MsaT011278.1:CDS
AAGCTTGCGGATAACAGTAT+GGG 0.608301 3:-745845 MsaT011278.1:CDS
GATCCGCCAGTATTGTCCAT+AGG 0.610382 3:+746820 None:intergenic
TCTCTTATCACAAAGACGCA+AGG 0.612018 3:-746430 MsaT011278.1:CDS
CATTGGCGGATCCACCAGAT+GGG 0.612044 3:-746405 MsaT011278.1:CDS
ACAACTTTCTTGATGCATGG+TGG 0.612301 3:+744786 None:intergenic
ACGGGACGCACCCATACGAT+GGG 0.612774 3:+743023 None:intergenic
GGTAAATTCTCGGACAAAGG+AGG 0.613744 3:-745630 MsaT011278.1:CDS
GAAACCCTATTGAGGTCCAG+TGG 0.614244 3:+746742 None:intergenic
GTATGGATTCACAGTCCCGT+TGG 0.614716 3:+745514 None:intergenic
TTCTCAACCCACAAACTCTG+TGG 0.618575 3:-749232 MsaT011278.1:CDS
AAAGACGCAAGGTACATTGG+CGG 0.619575 3:-746419 MsaT011278.1:CDS
GCACAAATAGAAAAGCATGT+CGG 0.620725 3:-745287 MsaT011278.1:CDS
AAACCCTATTGAGGTCCAGT+GGG 0.622694 3:+746743 None:intergenic
ATGTTGCAGAAACCCTATTG+AGG 0.623777 3:+746734 None:intergenic
CATATTAAAGTTACACACGC+TGG 0.624968 3:-744579 MsaT011278.1:CDS
ATGTTTCCACATGAATAGGA+CGG 0.627738 3:+743004 None:intergenic
ATGGTTGTAGATGTGTAAGT+TGG 0.627824 3:+744973 None:intergenic
AGTAGACTTTCCACATGCGA+AGG 0.629550 3:+744672 None:intergenic
GTCTACTGAATCCAAAACAG+TGG 0.630419 3:-744656 MsaT011278.1:CDS
ACATAAACATGCAATTGACC+GGG 0.631166 3:+745263 None:intergenic
AGTGGTAATAACACGGCTGG+TGG 0.634167 3:-746325 MsaT011278.1:CDS
CAGGAGACTGTATCTTATGG+AGG 0.634908 3:+744488 None:intergenic
AAAGTCCTGATTCAATGGCA+CGG 0.635118 3:-744217 MsaT011278.1:CDS
TTAGAGCACTCTTCTTACGT+GGG 0.635425 3:+745147 None:intergenic
CGAGATCAGTGGTAATAACA+CGG 0.636732 3:-746332 MsaT011278.1:CDS
ATTATTGATTCAACGCAATG+CGG 0.637376 3:-749079 MsaT011278.1:CDS
ACCTCCTGATGCTTGCTCTG+AGG 0.637926 3:-746152 MsaT011278.1:CDS
AGGCAGAGATGGAAGAAGGT+CGG 0.641182 3:-744875 MsaT011278.1:CDS
TGGTCTAGATTCTACTTCAG+GGG 0.641369 3:-744517 MsaT011278.1:CDS
GCTCACAGAGAGCCATACCA+AGG 0.642203 3:+745387 None:intergenic
TGTCAAATCTGAAGATGGTG+GGG 0.643211 3:-742714 MsaT011278.1:CDS
AATTTCGTAGGTGCAAGGAA+TGG 0.643325 3:-744349 MsaT011278.1:intron
TAGAGAAAGGTGTATACAGG+TGG 0.643392 3:-745454 MsaT011278.1:CDS
TCCTCAGAGCAAGCATCAGG+AGG 0.643607 3:+746151 None:intergenic
TGGGTAATTCTAATGCAGCA+TGG 0.644906 3:-745826 MsaT011278.1:CDS
ATGGCGTAATGAAAGGGAGT+CGG 0.645028 3:+744324 None:intergenic
TGTAACTCAATAAGATCTTG+GGG 0.649776 3:+744164 None:intergenic
TTGGAATGATCCTTCGCATG+TGG 0.651252 3:-744682 MsaT011278.1:CDS
CACTTGAAAGGAACTCCGGG+TGG 0.654146 3:-745707 MsaT011278.1:CDS
CCTACAGAACATGAATCATG+AGG 0.654419 3:+746187 None:intergenic
GTGGAAACATTAGCTATGCG+TGG 0.666167 3:-742991 MsaT011278.1:CDS
GTTTGGAGTACAGTGATACG+TGG 0.666295 3:-745898 MsaT011278.1:CDS
TGTGGCTATTACGAGATCAG+TGG 0.666939 3:-746343 MsaT011278.1:CDS
ACTACTGGTGACCCATCTGG+TGG 0.667483 3:+746394 None:intergenic
GCGGAATGACTTGTGTGCAA+AGG 0.667601 3:+743883 None:intergenic
GTCAAATCTGAAGATGGTGG+GGG 0.669627 3:-742713 MsaT011278.1:CDS
TCTATATTTCACAGATGACG+AGG 0.671046 3:-746077 MsaT011278.1:CDS
TTAGCCGTTTGAGTGCGGAG+TGG 0.671278 3:-745172 MsaT011278.1:CDS
ATACCTATGGACAATACTGG+CGG 0.671595 3:-746823 MsaT011278.1:CDS
GACCGTACCTTCCCTTGTGT+AGG 0.673251 3:-746877 MsaT011278.1:CDS
GTTGTCAAATCTGAAGATGG+TGG 0.674583 3:-742716 MsaT011278.1:CDS
CTCACAGAGAGCCATACCAA+GGG 0.681432 3:+745388 None:intergenic
AGGCACAACATATGTCGACA+TGG 0.683756 3:+744305 None:intergenic
AAGTTGCATATCTCGTCCAG+AGG 0.698811 3:-743987 MsaT011278.1:CDS
AAACAGTGGAAATTTCGTAG+TGG 0.699967 3:-744642 MsaT011278.1:CDS
AACTGGTGATATAGTCATCG+GGG 0.706873 3:-746530 MsaT011278.1:CDS
ACATTCCTGGAATGATGATG+CGG 0.709579 3:+743864 None:intergenic
TCTGCTGATTTCCTACACAA+GGG 0.709827 3:+746866 None:intergenic
GTTCTGGACAGAGTTACAAG+TGG 0.713387 3:-745943 MsaT011278.1:CDS
AAGTAGCATTAGTACCAATG+TGG 0.718006 3:-743839 MsaT011278.1:CDS
ATTTGCTGGAACAACTACTG+TGG 0.724132 3:+745328 None:intergenic
AAGTATATTACCTGACACAG+TGG 0.731295 3:+744457 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 742646 749278 742646 ID=MsaG011278
Chr3 mRNA 742646 749278 742646 ID=MsaT011278.1;Parent=MsaG011278
Chr3 exon 742646 742774 742646 ID=MsaT011278.1.exon9;Parent=MsaT011278.1
Chr3 CDS 742646 742774 742646 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Chr3 exon 742957 743071 742957 ID=MsaT011278.1.exon8;Parent=MsaT011278.1
Chr3 CDS 742957 743071 742957 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Chr3 exon 743347 743477 743347 ID=MsaT011278.1.exon7;Parent=MsaT011278.1
Chr3 CDS 743347 743477 743347 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Chr3 exon 743606 743665 743606 ID=MsaT011278.1.exon6;Parent=MsaT011278.1
Chr3 CDS 743606 743665 743606 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Chr3 exon 743777 744049 743777 ID=MsaT011278.1.exon5;Parent=MsaT011278.1
Chr3 CDS 743777 744049 743777 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Chr3 exon 744148 744361 744148 ID=MsaT011278.1.exon4;Parent=MsaT011278.1
Chr3 CDS 744148 744361 744148 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Chr3 exon 744468 746898 744468 ID=MsaT011278.1.exon3;Parent=MsaT011278.1
Chr3 CDS 744468 746898 744468 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Chr3 exon 746949 747073 746949 ID=MsaT011278.1.exon2;Parent=MsaT011278.1
Chr3 CDS 746949 747073 746949 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Chr3 exon 748824 749278 748824 ID=MsaT011278.1.exon1;Parent=MsaT011278.1
Chr3 CDS 748824 749278 748824 ID=cds.MsaT011278.1;Parent=MsaT011278.1
Gene Sequence

>MsaT011278.1

ATGACCACCACCAACAACGACCCTTTCTCAACCCACAAACTCTGTGGTTTTCTCTCAACCGTTCTCACACTAACCCCTCCGAATCAAAACGACACGCGTTTTAACGATCGATGCGAATTCTTCAACGACAACAATACAGTCGCGTTTCGGACTCAAAACGGTGTCGTTTTGAATCCAATTATTGATTCAACGCAATGCGGCGAGAGTTCGAGTGCGAAGAAGGTGAAGAAGGTTGGGATGGTGAATGGGAGTTTCAGTGTTGTGAATCAGCTTCATGCGTTGGTTTTGAGGAAATGTTTGGATATTGATGCACGTGTTTTGTGTGTTGTGGAGGTTGATTCTAGGGTTAGGGTTGTTGTGATTGTTGATGTTTATCTTCCGGTTTTGGTTTGGAGTAGTGGTTGGCAGTTTCCGAAATCTGGTTCTGTTGCTGGTGCTGTTTTTCGACATTTGAGCTGTGATTGGAATGAGAGAAGTTCAATGCTTTCTGATCCAGACTACTGTAGAAAGATACTTGGAGAAAATGAGAGCATTTGGAATCTTTCTGATTGTCATGTACTTGGTTGCAAGCTCCATTCCTACCGTACCTTCCCTTGTGTAGGAAATCAGCAGAGCTTTCTTATTTCCAAGATAATACCTATGGACAATACTGGCGGATCTGGGATTTGGGATATCTCTGATGATATTTTAATTAAAATTCTATATTCCCTGGGCCCACTGGACCTCAATAGGGTTTCTGCAACATGTCATCATCTAAGAGCCTTGGCTGCTTCAGTAATTCCTTGCACAAAGTTAAATCTGTTTCCTCATCAGCAGGCAGCAGTTGAATGGATGTTGCAACGTGAACGAAATGCTGAACTTATGCCACATCCTTTATATGCAGCTCTATCAACTGATGATGGCTTTAGTTTCCATGTAAATACTGTAACTGGTGATATAGTCATCGGGGAAACTCCCACTATCAAAGATTTTCGTGGAGGAATGTTTTGTGATGAGCCTGGCTTGGGTAAGACTGTAACAGCTCTTTCTCTTATCACAAAGACGCAAGGTACATTGGCGGATCCACCAGATGGGTCACCAGTAGTCTGGTGTCAACATAACACTAACAAAAAATGTGGCTATTACGAGATCAGTGGTAATAACACGGCTGGTGGTTATACTATTATGGGTAAGAGGGATGTGTGCCAAGATTCAGGTAGAAGTAATGAGAACCATGATTTCTCCTCTGAAAGAGCCAAATTGTTAAATCCTGATCAGGAAATCACTAAACCTCATGATTCATGTTCTGTAGGAGAAGACAAATCACCTCCTGATGCTTGCTCTGAGGAATATTCGCCTGCGAGTCGGTGCACTAGAAGCTTGAGTCGCGTAAAGAAAAATCTATATTTCACAGATGACGAGGAAGCTATGATTTCCAACGAAAGAAGAGTTGGCAAAAGACCAATTAAAACAAAACATGCATCAGATGTTGCATCCCAGATGTCTCAAAACAAGCTTGTTGATACTTCATATGGTTCTGGACAGAGTTACAAGTGGCATGGGAAGCGTAAAGTTGATTGTTTGGAGTACAGTGATACGTGGATTCAATGTGATGCTTGTCACAAATGGCGAAAGCTTGCGGATAACAGTATGGGTAATTCTAATGCAGCATGGTTTTGTAGTATGAACACTGACCCTTTGTATCAGAGTTGTAGAGTTCCAGAACAGTCTTTTAAGAACAGCTCTAAGATAACTTACTTACCAGGATTTCACTTGAAAGGAACTCCGGGTGGTGAGAAACAAAACGTTTCATTTTTCACTAGTGTGCTTAAGGAGCACTACTCATTGGTAAATTCTCGGACAAAGGAGGCTCTGACTTGGTTGGCCAAAATTTCTATTGACAAGCTTGCAGAAATGGAAACAAATGGAATACGAAGTCCTATTTTAAACAACTGCACTTTGTCCAACGGGACTGTGAATCCATACCACAAAGTATTTCAAGCATTTGGCCTAAAAAAGAAAGTAGAGAAAGGTGTATACAGGTGGTTCTACCCAAAAAATCTTAAAAATTTGACTTTTGATGTGGCTGCCCTTGGTATGGCTCTCTGTGAGCCATTAGATTTGGTTAGGTTATACTTGTCAAGAGCAACCACAGTAGTTGTTCCAGCAAATTTGGTTGATCATTGGAAAGCACAAATAGAAAAGCATGTCGGGCCCGGTCAATTGCATGTTTATGTTTGGAACGATCATCGGAAACCATCTGCGCACTCGCTTGCCTGGGATTATGATGTTGTCATCACTACATTTAGCCGTTTGAGTGCGGAGTGGGGCCCACGTAAGAAGAGTGCTCTAATGCAAGTGCACTGGTTTAGGATAATTTTAGATGAAGGGCATACTCTTGGCTCAAGCCTGAGCTTAACAAACAAGTTGCAAATGACTATTTCATTGGTTGCTTCAAATCGGTGGATACTAACTGGAACTCCAACATCGAACACTCCTAACAGCCAACTTACACATCTACAACCATTGCTAAGGTTCCTTCATGAAGAAGCTTATGGACTGAATCAAAAATCATGGGATGCTGGCGTGCTTAGGCCATTCGAGGCAGAGATGGAAGAAGGTCGGTCTCGTCTGTTGCATTTACTTCAGAAATGCATGATTAGTGCTAGAAAGGCAGATTTGCAAAGTATTCCACCATGCATCAAGAAAGTTGTTTTACTAGATTTTAATGAGGAACATGCTAGAAGTTACAATGAGTTGGTACTCACTGTTCGGCGTAATATATTGATGGCTGATTGGAATGATCCTTCGCATGTGGAAAGTCTACTGAATCCAAAACAGTGGAAATTTCGTAGTGGAGCTATAAAAAATGTAAGGCTTTCATGCTGTGTTGCTGGACATATTAAAGTTACACACGCTGGAGAAGATATTCAGGAAACAATGGATATGTTAGTGAAAAATGGTCTAGATTCTACTTCAGGGGAGTATACCTCCATAAGATACAGTCTCCTGTTTGGTGGCCACTGTGTCAGGTGCAAGGAATGGTGCCGACTCCCTTTCATTACGCCATGTCGACATATGTTGTGCCTTGATTGTGTTTCCATTGACAAGACAAAGTGTACATATCCTGGCTGTGATAACTTGTATGAGATGCAAAGTCCTGATTCAATGGCACGGCCAGAAAATCCTAACCCAAAGTGGTCTGTACCCCAAGATCTTATTGAGTTACAACCTTCTTATGCGCAGGATAATTGGGATCCTGATTGGCAATCAACATCTAGTAGTAAAGTTGCATATCTCGTCCAGAGGTTGAAAGCTTTGCAGGGAATCAATGAAGAGATGAGTTCATATACTGACAATAGCAATGCGATGTATATTGAAAATAGTTTTCCTTTGCACACAAGTCATTCCGCATCATCATTCCAGGAATGTTCAAGTAGCATTAGTACCAATGTGGTCCCTGAAAAAGTTTTGATATTTTCTCAGTTTCTTGAGCATATACATGTCATTGAACAACAGTTGGCTGTTAATGGTATCAAATATACTGGAATGTATAACCCAATGCATTCTAGCAACAAGAAGAAGTCATTAGCCAAGTTCCAGCACGATTCAAGTTGTATGGCACTTCTAATGGATGGGAGTGCTGCATTGGGTCTTGACTTAAGTTTTGTTACACATGTATTTTTAATGGAGCCAATTTGGGACAGAAGTATGGAGGAGCAAGTAATTAGTCGTGCCCATCGTATGGGTGCGTCCCGTCCTATTCATGTGGAAACATTAGCTATGCGTGGTACAATTGAAGAGCAAATGCTAGAATTTTTACAGGATGCTGACAAACGTAGAAGATTTCATAATAAAGATGTTGTCAAATCTGAAGATGGTGGGGGACGAGGGTGTCGATCAGTGCATGATTTTGCTGAAAGCAATTATCTACTGGAACTTAAATTTGTATAA
Protein sequence

>MsaT011278.1

MTTTNNDPFSTHKLCGFLSTVLTLTPPNQNDTRFNDRCEFFNDNNTVAFRTQNGVVLNPIIDSTQCGESSSAKKVKKVGMVNGSFSVVNQLHALVLRKCLDIDARVLCVVEVDSRVRVVVIVDVYLPVLVWSSGWQFPKSGSVAGAVFRHLSCDWNERSSMLSDPDYCRKILGENESIWNLSDCHVLGCKLHSYRTFPCVGNQQSFLISKIIPMDNTGGSGIWDISDDILIKILYSLGPLDLNRVSATCHHLRALAASVIPCTKLNLFPHQQAAVEWMLQRERNAELMPHPLYAALSTDDGFSFHVNTVTGDIVIGETPTIKDFRGGMFCDEPGLGKTVTALSLITKTQGTLADPPDGSPVVWCQHNTNKKCGYYEISGNNTAGGYTIMGKRDVCQDSGRSNENHDFSSERAKLLNPDQEITKPHDSCSVGEDKSPPDACSEEYSPASRCTRSLSRVKKNLYFTDDEEAMISNERRVGKRPIKTKHASDVASQMSQNKLVDTSYGSGQSYKWHGKRKVDCLEYSDTWIQCDACHKWRKLADNSMGNSNAAWFCSMNTDPLYQSCRVPEQSFKNSSKITYLPGFHLKGTPGGEKQNVSFFTSVLKEHYSLVNSRTKEALTWLAKISIDKLAEMETNGIRSPILNNCTLSNGTVNPYHKVFQAFGLKKKVEKGVYRWFYPKNLKNLTFDVAALGMALCEPLDLVRLYLSRATTVVVPANLVDHWKAQIEKHVGPGQLHVYVWNDHRKPSAHSLAWDYDVVITTFSRLSAEWGPRKKSALMQVHWFRIILDEGHTLGSSLSLTNKLQMTISLVASNRWILTGTPTSNTPNSQLTHLQPLLRFLHEEAYGLNQKSWDAGVLRPFEAEMEEGRSRLLHLLQKCMISARKADLQSIPPCIKKVVLLDFNEEHARSYNELVLTVRRNILMADWNDPSHVESLLNPKQWKFRSGAIKNVRLSCCVAGHIKVTHAGEDIQETMDMLVKNGLDSTSGEYTSIRYSLLFGGHCVRCKEWCRLPFITPCRHMLCLDCVSIDKTKCTYPGCDNLYEMQSPDSMARPENPNPKWSVPQDLIELQPSYAQDNWDPDWQSTSSSKVAYLVQRLKALQGINEEMSSYTDNSNAMYIENSFPLHTSHSASSFQECSSSISTNVVPEKVLIFSQFLEHIHVIEQQLAVNGIKYTGMYNPMHSSNKKKSLAKFQHDSSCMALLMDGSAALGLDLSFVTHVFLMEPIWDRSMEEQVISRAHRMGASRPIHVETLAMRGTIEEQMLEFLQDADKRRRFHNKDVVKSEDGGGRGCRSVHDFAESNYLLELKFV*