AlfalfaGEDB | MsaG011315

M. sativa ssp. caerulea / MsaG011315

KEGG: K12606 K13754
GO: GO:0000003 GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005102 GO:0005154 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007044 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0007346 GO:0007548 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010562 GO:0010564 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010646 GO:0010647 GO:0010648 GO:0010948 GO:0016043 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019220 GO:0019221 GO:0019222 GO:0019439 GO:0019899 GO:0019900 GO:0019904 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030014 GO:0030015 GO:0030330 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031401 GO:0031570 GO:0031571 GO:0031581 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033135 GO:0033138 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034097 GO:0034243 GO:0034248 GO:0034249 GO:0034329 GO:0034330 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042058 GO:0042221 GO:0042325 GO:0042327 GO:0042770 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045742 GO:0045786 GO:0045787 GO:0045893 GO:0045930 GO:0045935 GO:0045937 GO:0045944 GO:0046483 GO:0046700 GO:0046983 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070851 GO:0070887 GO:0071156 GO:0071158 GO:0071310 GO:0071345 GO:0071704 GO:0071840 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:1901184 GO:1901186 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902680 GO:1902806 GO:1902807 GO:1903047 GO:1903506 GO:1903508 GO:1990904 GO:2000045 GO:2000112 GO:2000113 GO:2000134 GO:2000325 GO:2000327 GO:2001141
PFAM: PF04078.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011315	XP_003598636.1	95.017	301	15	0	1	301	1	301	0.0	579

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011315	sp\|Q6P819\|CNOT9_XENTR	51.724	261	126	0	43	303	28	288	3.49e-88	269

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011315	tr\|Q2HW81\|Q2HW81_MEDTR	95.017	301	15	0	1	301	1	301	0.0	579

TFs/TRs:

Gene ID	Type	Classification
MsaG011315	TR	Rcd1-like

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000138	MsaG011315	0.817708	4.991325e-52	1.033952e-49
MsaG000705	MsaG011315	0.804305	3.884511e-49	5.795122e-47
MsaG001045	MsaG011315	0.811931	9.389932e-51	1.681160e-48
MsaG001270	MsaG011315	0.814702	2.327766e-51	4.466034e-49
MsaG002142	MsaG011315	0.813424	4.441221e-51	8.252213e-49
MsaG002298	MsaG011315	0.810553	1.863063e-50	3.224765e-48
MsaG002804	MsaG011315	0.815054	1.947104e-51	3.768939e-49
MsaG002785	MsaG011315	0.818926	2.653066e-52	5.672415e-50
MsaG003206	MsaG011315	0.821761	5.981985e-53	1.378438e-50
MsaG004010	MsaG011315	0.823757	2.061867e-53	5.014193e-51
MsaG004091	MsaG011315	0.805081	2.679436e-49	4.069836e-47
MsaG004099	MsaG011315	0.823096	2.938562e-53	7.018925e-51
MsaG004329	MsaG011315	0.811410	1.217419e-50	2.151873e-48
MsaG004455	MsaG011315	0.803872	4.775395e-49	7.052718e-47
MsaG005038	MsaG011315	0.803274	6.343309e-49	9.239948e-47
MsaG005226	MsaG011315	0.832245	1.908237e-55	5.892535e-53
MsaG006129	MsaG011315	0.818958	2.609222e-52	5.583355e-50
MsaG006284	MsaG011315	0.808416	5.330513e-50	8.761886e-48
MsaG006476	MsaG011315	0.807994	6.552936e-50	1.066256e-47
MsaG006548	MsaG011315	0.821349	7.437318e-53	1.695180e-50
MsaG007079	MsaG011315	0.806646	1.260760e-49	1.986696e-47
MsaG007146	MsaG011315	0.808550	4.993685e-50	8.234733e-48
MsaG008125	MsaG011315	0.828801	1.315679e-54	3.679590e-52
MsaG008919	MsaG011315	0.804427	3.664903e-49	5.482809e-47
MsaG009423	MsaG011315	0.810531	1.883079e-50	3.257792e-48
MsaG009875	MsaG011315	0.800676	2.156334e-48	2.960452e-46
MsaG010113	MsaG011315	0.814971	2.030876e-51	3.923052e-49
MsaG010303	MsaG011315	0.826639	4.328442e-54	1.139392e-51
MsaG010307	MsaG011315	0.800481	2.362101e-48	3.228589e-46
MsaG010363	MsaG011315	0.805012	2.769936e-49	4.200601e-47
MsaG010604	MsaG011315	0.809654	2.903548e-50	4.917279e-48
MsaG011147	MsaG011315	0.823732	2.089578e-53	5.078128e-51
MsaG011213	MsaG011315	0.806206	1.559667e-49	2.432368e-47
MsaG011315	MsaG012334	0.819479	1.988371e-52	4.313375e-50
MsaG011315	MsaG012396	0.810590	1.829142e-50	3.168910e-48
MsaG011315	MsaG014305	0.824180	1.642518e-53	4.040273e-51
MsaG011315	MsaG014486	0.824742	1.213382e-53	3.030748e-51
MsaG011315	MsaG015144	0.813010	5.471042e-51	1.006081e-48
MsaG011315	MsaG015237	0.860746	3.281656e-63	2.625407e-60
MsaG011315	MsaG015854	0.824053	1.759003e-53	4.312034e-51
MsaG011315	MsaG016056	0.803494	5.715145e-49	8.367143e-47
MsaG011315	MsaG016118	0.806391	1.426641e-49	2.234592e-47
MsaG011315	MsaG016159	0.842381	4.981517e-58	2.097057e-55
MsaG011315	MsaG016179	0.834198	6.259639e-56	2.046996e-53
MsaG011315	MsaG016384	0.867893	1.973786e-65	2.096267e-62
MsaG011315	MsaG016503	0.838486	5.140108e-57	1.913852e-54
MsaG011315	MsaG017561	0.808560	4.967851e-50	8.194303e-48
MsaG011315	MsaG017887	0.814235	2.949753e-51	5.593539e-49
MsaG011315	MsaG017973	0.808690	4.663032e-50	7.715811e-48
MsaG011315	MsaG018849	0.803027	7.133722e-49	1.033199e-46
MsaG011315	MsaG018958	0.801922	1.201765e-48	1.697088e-46
MsaG011315	MsaG019435	0.831135	3.572128e-55	1.067972e-52
MsaG011315	MsaG019606	0.822484	4.073850e-53	9.571132e-51
MsaG011315	MsaG020730	0.820613	1.096568e-52	2.451125e-50
MsaG011315	MsaG021090	0.819533	1.932799e-52	4.198603e-50
MsaG011315	MsaG021888	0.823731	2.091018e-53	5.081390e-51
MsaG011315	MsaG022303	0.803489	5.729648e-49	8.387389e-47
MsaG011315	MsaG022880	0.851102	2.108701e-60	1.187133e-57
MsaG011315	MsaG023078	0.854066	3.038080e-61	1.898362e-58
MsaG011315	MsaG023997	0.840024	2.060939e-57	8.051712e-55
MsaG011315	MsaG025448	0.812290	7.849090e-51	1.417696e-48
MsaG011315	MsaG025838	0.859780	6.405200e-63	4.938680e-60
MsaG011315	MsaG025985	0.832704	1.470013e-55	4.600466e-53
MsaG011315	MsaG026446	0.806382	1.432724e-49	2.243643e-47
MsaG011315	MsaG026826	0.807798	7.208758e-50	1.167450e-47
MsaG011315	MsaG027432	0.822955	3.168793e-53	7.540156e-51
MsaG011315	MsaG027543	0.834837	4.332752e-56	1.444254e-53
MsaG011315	MsaG027547	0.844402	1.446031e-58	6.496923e-56
MsaG011315	MsaG027759	0.815104	1.897546e-51	3.677712e-49
MsaG011315	MsaG027995	0.821615	6.461661e-53	1.483227e-50
MsaG011315	MsaG028181	0.802821	7.862127e-49	1.133286e-46
MsaG011315	MsaG028483	0.830117	6.322697e-55	1.835998e-52
MsaG011315	MsaG028585	0.840502	1.547926e-57	6.138636e-55
MsaG011315	MsaG028762	0.804173	4.137431e-49	6.153464e-47
MsaG011315	MsaG029029	0.830669	4.641393e-55	1.369148e-52
MsaG011315	MsaG029076	0.815913	1.255896e-51	2.484563e-49
MsaG011315	MsaG029377	0.811315	1.276200e-50	2.250569e-48
MsaG011315	MsaG029403	0.808424	5.310485e-50	8.730682e-48
MsaG011315	MsaG030197	0.849696	5.208983e-60	2.794182e-57
MsaG011315	MsaG030238	0.804589	3.391349e-49	5.092612e-47
MsaG011315	MsaG030451	0.835039	3.856127e-56	1.293199e-53
MsaG011315	MsaG030455	0.853928	3.327283e-61	2.068863e-58
MsaG011315	MsaG030494	0.804618	3.344844e-49	5.026113e-47
MsaG011315	MsaG031104	0.810873	1.589731e-50	2.773192e-48
MsaG011315	MsaG031527	0.842350	5.076392e-58	2.134810e-55
MsaG011315	MsaG032102	0.808019	6.471422e-50	1.053645e-47
MsaG011315	MsaG032564	0.849422	6.206128e-60	3.298238e-57
MsaG011315	MsaG033412	0.817308	6.135372e-52	1.258006e-49
MsaG011315	MsaG033421	0.802119	1.095420e-48	1.553805e-46
MsaG011315	MsaG034018	0.853076	5.828552e-61	3.516287e-58
MsaG011315	MsaG035263	0.820572	1.120525e-52	2.501996e-50
MsaG011315	MsaG035271	0.806113	1.631402e-49	2.538499e-47
MsaG011315	MsaG036489	0.812675	6.472180e-51	1.180279e-48
MsaG011315	MsaG036828	0.804247	3.992796e-49	5.948668e-47
MsaG011315	MsaG037804	0.800013	2.937707e-48	3.973285e-46
MsaG011315	MsaG038520	0.817289	6.195464e-52	1.269729e-49
MsaG011315	MsaG038522	0.821841	5.731339e-53	1.323509e-50
MsaG011315	MsaG039139	0.811906	9.507226e-51	1.701095e-48
MsaG011315	MsaG040511	0.830511	5.071047e-55	1.489135e-52
MsaG011315	MsaG040685	0.806128	1.619267e-49	2.520577e-47
MsaG011315	MsaG040748	0.817883	4.560552e-52	9.490163e-50
MsaG011315	MsaG042534	0.827543	2.635577e-54	7.115632e-52
MsaG011315	MsaG042720	0.815275	1.739290e-51	3.385737e-49
MsaG011315	MsaG042932	0.804370	3.766226e-49	5.627046e-47
MsaG011315	MsaG043183	0.821585	6.563759e-53	1.505437e-50
MsaG011315	MsaG043229	0.819126	2.390319e-52	5.137642e-50
MsaG011315	MsaG043847	0.811556	1.132165e-50	2.008365e-48
MsaG011315	MsaG044050	0.803073	6.979241e-49	1.011912e-46
MsaG011315	MsaG044115	0.810276	2.136915e-50	3.673830e-48
MsaG011315	MsaG044630	0.806212	1.555554e-49	2.426266e-47
MsaG011315	MsaG044709	0.807981	6.594073e-50	1.072616e-47
MsaG011315	MsaG044865	0.848391	1.196047e-59	6.136426e-57
MsaG011315	MsaG045052	0.800180	2.718821e-48	3.690829e-46
MsaG011315	MsaG046278	0.812548	6.898292e-51	1.254049e-48
MsaG011315	MsaG046438	0.802838	7.800311e-49	1.124801e-46
MsaG011315	MsaG046828	0.813324	4.672421e-51	8.659640e-49
MsaG011315	MsaG046830	0.832357	1.790750e-55	5.547759e-53
MsaG011315	MsaG046974	0.822277	4.547082e-53	1.062358e-50
MsaG011315	MsaG002665	0.809287	3.478015e-50	5.837921e-48
MsaG011315	MsaG000822	0.845788	6.133490e-59	2.884441e-56
MsaG011315	MsaG009146	0.880100	1.530046e-69	2.773920e-66
MsaG011315	MsaG008489	0.809905	2.566336e-50	4.372555e-48
MsaG011315	MsaG008774	0.837690	8.220828e-57	2.986411e-54
MsaG011315	MsaG012002	0.802361	9.770459e-49	1.393681e-46
MsaG011315	MsaG019332	0.807674	7.656826e-50	1.236310e-47
MsaG011315	MsaG027150	0.844156	1.683065e-58	7.501332e-56
MsaG011315	MsaG031514	0.863800	3.822454e-64	3.442869e-61
MsaG011315	MsaG032454	0.810930	1.545322e-50	2.699509e-48
MsaG011315	MsaG032963	0.809342	3.386744e-50	5.692103e-48
MsaG011315	MsaG036777	0.803032	7.114220e-49	1.030500e-46
MsaG011315	MsaG043110	0.811087	1.429680e-50	2.507136e-48
MsaG011315	MsaG043535	0.822673	3.682365e-53	8.695756e-51
MsaG011315	MsaG043553	0.829677	8.084450e-55	2.318154e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011315	MtrunA17_Chr7g0224601	62.046	303	111	2	12	310	2	304	7.66e-120	347
MsaG011315	MtrunA17_Chr8g0392781	51.685	356	137	2	1	321	1	356	7.47e-115	336
MsaG011315	MtrunA17_Chr1g0177151	60.700	257	97	1	1	257	1	253	3.25e-112	326
MsaG011315	MtrunA17_Chr3g0099561	58.781	279	115	0	38	316	26	304	6.09e-110	322
MsaG011315	MtrunA17_Chr5g0444621	62.381	210	79	0	70	279	52	261	1.13e-86	261
MsaG011315	MtrunA17_Chr6g0477171	61.809	199	76	0	59	257	45	243	1.02e-81	248
MsaG011315	MtrunA17_Chr3g0083811	97.414	116	3	0	186	301	1	116	2.68e-76	229
MsaG011315	MtrunA17_Chr3g0083821	72.072	111	31	0	54	164	10	120	1.92e-50	164
MsaG011315	MtrunA17_Chr5g0444631	64.151	53	19	0	112	164	4	56	6.83e-18	76.6
MsaG011315	MtrunA17_Chr6g0477181	59.322	59	24	0	1	59	1	59	2.20e-14	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011315	AT3G20800.1	53.035	313	141	2	9	316	5	316	1.26e-107	317
MsaG011315	AT5G12980.1	52.727	275	129	1	31	304	28	302	3.63e-97	290
MsaG011315	AT2G32550.2	35.918	245	141	5	53	285	67	307	5.90e-37	135
MsaG011315	AT2G32550.3	36.325	234	133	5	64	285	39	268	1.20e-36	133
MsaG011315	AT2G32550.1	36.111	216	122	5	82	285	2	213	7.19e-32	119

Find 58 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAACTTGGACCTTTATTA+TGG	0.221177	3:-1257644	MsaT011315.1:CDS
ATAGAAACCTATGGCTTCTT+TGG	0.275780	3:+1235891	None:intergenic
CTCGCTTATCTCAAAATTGC+AGG	0.298861	3:-1235528	MsaT011315.1:intron
AGAAATGAGATCCTTGTATC+AGG	0.301701	3:+1243661	None:intergenic
TTGGCATCTTTGAGCATGTT+TGG	0.352246	3:+1235386	None:intergenic
GTTGCTACTCTTGGAGTCAT+TGG	0.359762	3:-1243225	MsaT011315.1:CDS
AGGAATGGATATAAATATAA+AGG	0.370332	3:+1243288	None:intergenic
TATATTAGACGATGATGGTT+TGG	0.376298	3:-1235671	MsaT011315.1:CDS
CCAACAGTATTCCATAATAA+AGG	0.383147	3:+1257633	None:intergenic
TATGATCAGTGGTGCTAGAT+TGG	0.401740	3:+1258750	None:intergenic
AATTTGAGGGTTGCTACTCT+TGG	0.407238	3:-1243234	MsaT011315.1:CDS
TGTTGTTACATGAGAATGTT+GGG	0.422120	3:-1228638	MsaT011315.1:CDS
TCAGAGCACCAAGTTATTTG+AGG	0.428345	3:-1257664	MsaT011315.1:intron
GATTGTGAGGGAGAGAGATT+CGG	0.440290	3:+1243930	None:intergenic
TAACACACTTTGAGAATTTG+AGG	0.446207	3:-1243248	MsaT011315.1:CDS
GGAGGAATTGATGATATTGT+AGG	0.453082	3:-1228585	MsaT011315.1:CDS
TTCTTTGTTGAGGGCGATTG+AGG	0.456697	3:+1258809	None:intergenic
GATGTATCGTTCTTTGTTGA+GGG	0.456766	3:+1258800	None:intergenic
ATGATCAGTGGTGCTAGATT+GGG	0.463771	3:+1258751	None:intergenic
AATCTAGCACCACTGATCAT+AGG	0.464495	3:-1258748	MsaT011315.1:CDS
AAAATTATATTAGACGATGA+TGG	0.467412	3:-1235676	MsaT011315.1:CDS
CACCCTCACCTCGTTTGTTG+AGG	0.472935	3:-1235570	MsaT011315.1:CDS
GCTACTCTTGGAGTCATTGG+TGG	0.479008	3:-1243222	MsaT011315.1:CDS
TGTTATTGAACTCAGCAATC+CGG	0.481393	3:-1258705	MsaT011315.1:CDS
ACCAAGTTATTTGAGGAACT+TGG	0.485648	3:-1257657	MsaT011315.1:CDS
AGATTCGGTGGTGAAAGAGT+AGG	0.488495	3:+1243945	None:intergenic
AACCGAGTTCCTTTAGAAGC+AGG	0.491153	3:-1228612	MsaT011315.1:CDS
CCTTTATTATGGAATACTGT+TGG	0.492405	3:-1257633	MsaT011315.1:CDS
CCTCCTCTACCTGCTTCTAA+AGG	0.503277	3:+1228603	None:intergenic
CGATGTATCGTTCTTTGTTG+AGG	0.511206	3:+1258799	None:intergenic
AACACACTTTGAGAATTTGA+GGG	0.516437	3:-1243247	MsaT011315.1:CDS
GTTCCTTTAGAAGCAGGTAG+AGG	0.520910	3:-1228606	MsaT011315.1:CDS
TCCAAGTTCCTCAAATAACT+TGG	0.522228	3:+1257656	None:intergenic
TGGCATCTCACCCTGATACA+AGG	0.537570	3:-1243672	MsaT011315.1:CDS
TGCTTGTCTAACATGCAAAA+CGG	0.538800	3:-1235844	MsaT011315.1:CDS
CTGTTGTTACATGAGAATGT+TGG	0.540471	3:-1228639	MsaT011315.1:CDS
CAATGTTCTTGCATTACTGC+AGG	0.546119	3:-1243895	MsaT011315.1:intron
AGTCATTGGTGGCTCGATGA+AGG	0.546457	3:-1243211	MsaT011315.1:intron
GAGGAATTGATGATATTGTA+GGG	0.550926	3:-1228584	MsaT011315.1:CDS
CCTTTAGAAGCAGGTAGAGG+AGG	0.553169	3:-1228603	MsaT011315.1:CDS
AGCCTCAACAAACGAGGTGA+GGG	0.559488	3:+1235568	None:intergenic
ACAAGGATCTCATTTCTCAA+TGG	0.566776	3:-1243655	MsaT011315.1:intron
AAGCCTCAACAAACGAGGTG+AGG	0.569309	3:+1235567	None:intergenic
ACTTGAGAGATAGAAACCTA+TGG	0.574907	3:+1235882	None:intergenic
TGCTCTCTATGTGCTCTCCA+AGG	0.584461	3:-1258669	MsaT011315.1:intron
CTAGATTGGGTAGAATCACC+AGG	0.589884	3:+1258764	None:intergenic
GAAATGAGATCCTTGTATCA+GGG	0.596244	3:+1243662	None:intergenic
CACACTCGAGTTGATTGTGA+GGG	0.603583	3:+1243918	None:intergenic
GTTAACACCAAAGAAGCCAT+AGG	0.615742	3:-1235898	MsaT011315.1:CDS
TAGCACCACTGATCATAGGA+TGG	0.627844	3:-1258744	MsaT011315.1:CDS
CTCAACAAACGAGGTGAGGG+TGG	0.629401	3:+1235571	None:intergenic
TACCTGCTTCTAAAGGAACT+CGG	0.631809	3:+1228610	None:intergenic
GATTGCTGAGTTCAATAACA+AGG	0.649486	3:+1258708	None:intergenic
ATCAAAAGCCTCAACAAACG+AGG	0.660997	3:+1235562	None:intergenic
GCACACTCGAGTTGATTGTG+AGG	0.665692	3:+1243917	None:intergenic
GTAAGCCATCCTATGATCAG+TGG	0.669763	3:+1258739	None:intergenic
TGTGAGGGAGAGAGATTCGG+TGG	0.689243	3:+1243933	None:intergenic
GTTGTTACATGAGAATGTTG+GGG	0.746517	3:-1228637	MsaT011315.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	1228563	1258846	1228563	ID=MsaG011315
Chr3	mRNA	1228563	1258846	1228563	ID=MsaT011315.1;Parent=MsaG011315
Chr3	exon	1228563	1228688	1228563	ID=MsaT011315.1.exon9;Parent=MsaT011315.1
Chr3	CDS	1228563	1228688	1228563	ID=cds.MsaT011315.1;Parent=MsaT011315.1
Chr3	exon	1235367	1235436	1235367	ID=MsaT011315.1.exon8;Parent=MsaT011315.1
Chr3	CDS	1235367	1235436	1235367	ID=cds.MsaT011315.1;Parent=MsaT011315.1
Chr3	exon	1235529	1235719	1235529	ID=MsaT011315.1.exon7;Parent=MsaT011315.1
Chr3	CDS	1235529	1235719	1235529	ID=cds.MsaT011315.1;Parent=MsaT011315.1
Chr3	exon	1235825	1235920	1235825	ID=MsaT011315.1.exon6;Parent=MsaT011315.1
Chr3	CDS	1235825	1235920	1235825	ID=cds.MsaT011315.1;Parent=MsaT011315.1
Chr3	exon	1243212	1243318	1243212	ID=MsaT011315.1.exon5;Parent=MsaT011315.1
Chr3	CDS	1243212	1243318	1243212	ID=cds.MsaT011315.1;Parent=MsaT011315.1
Chr3	exon	1243656	1243698	1243656	ID=MsaT011315.1.exon4;Parent=MsaT011315.1
Chr3	CDS	1243656	1243698	1243656	ID=cds.MsaT011315.1;Parent=MsaT011315.1
Chr3	exon	1243896	1243985	1243896	ID=MsaT011315.1.exon3;Parent=MsaT011315.1
Chr3	CDS	1243896	1243985	1243896	ID=cds.MsaT011315.1;Parent=MsaT011315.1
Chr3	exon	1257611	1257682	1257611	ID=MsaT011315.1.exon2;Parent=MsaT011315.1
Chr3	CDS	1257611	1257682	1257611	ID=cds.MsaT011315.1;Parent=MsaT011315.1
Chr3	exon	1258670	1258846	1258670	ID=MsaT011315.1.exon1;Parent=MsaT011315.1
Chr3	CDS	1258670	1258846	1258670	ID=cds.MsaT011315.1;Parent=MsaT011315.1

Gene Sequence

>MsaT011315.1

ATGGCACACTTTACTCCTCAATCGCCCTCAACAAAGAACGATACATCGTTTATAGCTTTTCCTGGTGATTCTACCCAATCTAGCACCACTGATCATAGGATGGCTTACATGCAAAGCCTTGTTATTGAACTCAGCAATCCGGATCTTAGAGAAAATGCTCTCTATGTGCTCTCCAAGAGCACCAAGTTATTTGAGGAACTTGGACCTTTATTATGGAATACTGTTGGTACTATTGCAGTATTTTTACAGGAAATAATTTCAACATATCCTACTCTTTCACCACCGAATCTCTCTCCCTCACAATCAACTCGAGTGTGCAATGTTCTTGCATTACTGCAGTGTGTGGCATCTCACCCTGATACAAGGATCTCATTTCTCAATGCTAATATGCCTTTATATTTATATCCATTCCTGAAAACGACAAGCACGTTAACACACTTTGAGAATTTGAGGGTTGCTACTCTTGGAGTCATTGGTGGCTCGATGAAGGTTAACACCAAAGAAGCCATAGGTTTCTATCTCTCAAGTGAGATAATTTCACTATGCTTGTCTAACATGCAAAACGGCAATGAATTGACAAAAATTGTTGCAACATTTATAATTCTGAAAATTATATTAGACGATGATGGTTTGGCTCGTGTTTGTGCTACAACAGAGAATTTTTTTGGAGTATGTCGAGTTTTGAACATGATGTTGGAAGATCTTGAGAATCCACCCTCACCTCGTTTGTTGAGGCTTTTGATTTCATGTTATTCTCGCTTATCTCAAAATTGCAGGGCTAGAGTTGCATTAACAACTTATCTTCCAAACATGCTCAAAGATGCCAATCTCATCAATTTTTTTCGTGATGATCCAAAAACTATGAGGTTGGTACTGTTGTTACATGAGAATGTTGGGGTGAACCGAGTTCCTTTAGAAGCAGGTAGAGGAGGAATTGATGATATTGTAGGGCCATCATTATCAAGAAAATGA

Protein sequence

>MsaT011315.1

MAHFTPQSPSTKNDTSFIAFPGDSTQSSTTDHRMAYMQSLVIELSNPDLRENALYVLSKSTKLFEELGPLLWNTVGTIAVFLQEIISTYPTLSPPNLSPSQSTRVCNVLALLQCVASHPDTRISFLNANMPLYLYPFLKTTSTLTHFENLRVATLGVIGGSMKVNTKEAIGFYLSSEIISLCLSNMQNGNELTKIVATFIILKIILDDDGLARVCATTENFFGVCRVLNMMLEDLENPPSPRLLRLLISCYSRLSQNCRARVALTTYLPNMLKDANLINFFRDDPKTMRLVLLLHENVGVNRVPLEAGRGGIDDIVGPSLSRK*