AlfalfaGEDB | MsaG011317

M. sativa ssp. caerulea / MsaG011317

KEGG: K12606 K13754
GO: GO:0000003 GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005102 GO:0005154 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007044 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0007346 GO:0007548 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010562 GO:0010564 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010646 GO:0010647 GO:0010648 GO:0010948 GO:0016043 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019220 GO:0019221 GO:0019222 GO:0019439 GO:0019899 GO:0019900 GO:0019904 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030014 GO:0030015 GO:0030330 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031401 GO:0031570 GO:0031571 GO:0031581 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033135 GO:0033138 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034097 GO:0034243 GO:0034248 GO:0034249 GO:0034329 GO:0034330 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042058 GO:0042221 GO:0042325 GO:0042327 GO:0042770 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045742 GO:0045786 GO:0045787 GO:0045893 GO:0045930 GO:0045935 GO:0045937 GO:0045944 GO:0046483 GO:0046700 GO:0046983 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070851 GO:0070887 GO:0071156 GO:0071158 GO:0071310 GO:0071345 GO:0071704 GO:0071840 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:1901184 GO:1901186 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902680 GO:1902806 GO:1902807 GO:1903047 GO:1903506 GO:1903508 GO:1990904 GO:2000045 GO:2000112 GO:2000113 GO:2000134 GO:2000325 GO:2000327 GO:2001141
PFAM: PF04078.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011317	XP_003598636.1	93.355	301	17	1	1	298	1	301	0.0	565

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011317	sp\|Q6P819\|CNOT9_XENTR	53.257	261	122	0	40	300	28	288	1.36e-91	277

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011317	tr\|Q2HW81\|Q2HW81_MEDTR	93.355	301	17	1	1	298	1	301	0.0	565

TFs/TRs:

Gene ID	Type	Classification
MsaG011317	TR	Rcd1-like

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG009010	MsaG011317	0.802737	8.181546e-49	1.177066e-46
MsaG011317	MsaG031787	0.803710	5.156254e-49	7.586984e-47
MsaG011317	MsaG037920	0.801980	1.169318e-48	1.653462e-46
MsaG011317	MsaG045881	0.826187	5.539662e-54	1.440039e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011317	MtrunA17_Chr7g0224601	62.376	303	107	3	12	307	2	304	6.35e-119	344
MsaG011317	MtrunA17_Chr1g0177151	62.205	254	95	1	1	254	1	253	1.44e-114	331
MsaG011317	MtrunA17_Chr8g0392781	53.333	345	123	3	1	307	1	345	6.25e-114	333
MsaG011317	MtrunA17_Chr3g0099561	60.584	274	107	1	35	307	26	299	1.72e-109	320
MsaG011317	MtrunA17_Chr5g0444621	62.857	210	78	0	67	276	52	261	4.00e-88	264
MsaG011317	MtrunA17_Chr6g0477171	65.306	196	68	0	59	254	48	243	1.96e-85	257
MsaG011317	MtrunA17_Chr3g0083811	95.690	116	5	0	183	298	1	116	5.15e-75	225
MsaG011317	MtrunA17_Chr3g0083821	72.973	111	30	0	51	161	10	120	2.92e-51	165
MsaG011317	MtrunA17_Chr5g0444631	67.925	53	17	0	109	161	4	56	2.04e-19	80.5
MsaG011317	MtrunA17_Chr6g0477181	57.627	59	22	1	1	56	1	59	5.89e-12	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011317	AT3G20800.1	57.692	286	121	0	22	307	26	311	2.40e-111	325
MsaG011317	AT5G12980.1	53.455	275	127	1	28	301	28	302	2.05e-99	295
MsaG011317	AT2G32550.2	35.510	245	142	5	50	282	67	307	4.35e-36	132
MsaG011317	AT2G32550.3	35.897	234	134	5	61	282	39	268	6.92e-36	130
MsaG011317	AT2G32550.1	35.648	216	123	5	79	282	2	213	6.74e-31	116

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAACTTGGACCTTTATTA+TGG	0.221177	3:-1293567	MsaT011317.1:CDS
CTCGCTTATCTCAAAATTGC+AGG	0.298861	3:-1290639	MsaT011317.1:intron
AGAAATGAGATCCTTGTATC+AGG	0.301701	3:+1292558	None:intergenic
ATAGAAACCTATGACTTCTT+TGG	0.317203	3:+1291002	None:intergenic
TTGGCATCTTTGAGCTTGTT+TGG	0.336372	3:+1290497	None:intergenic
AGGAATGGATATAAATATAA+AGG	0.360461	3:+1292168	None:intergenic
CAATGTTCTTGCACTACTTC+AGG	0.367374	3:-1292788	MsaT011317.1:intron
GTTGCTAGTCTTGGAGTCAT+TGG	0.372727	3:-1292105	MsaT011317.1:CDS
TATATTAGACGATGATGGTT+TGG	0.376298	3:-1290782	MsaT011317.1:CDS
CCAACAGTATTCCATAATAA+AGG	0.383147	3:+1293556	None:intergenic
GTTAACACCAAAGAAGTCAT+AGG	0.420089	3:-1291009	MsaT011317.1:CDS
TGTTGTTACATGAGAATGTT+GGG	0.422120	3:-1290009	MsaT011317.1:CDS
TCAGAGCACCAAGTTATTTG+AGG	0.428345	3:-1293587	MsaT011317.1:intron
TTCTTTGTTGAGAGCGATTG+AGG	0.439462	3:+1294971	None:intergenic
GATTGTGAGGGAGAGAGATT+CGG	0.440290	3:+1292823	None:intergenic
TAACACAGTTTGAGAATTTG+AGG	0.440707	3:-1292128	MsaT011317.1:CDS
ATTCTAACACCACTGATCAT+AGG	0.442748	3:-1294919	MsaT011317.1:CDS
AATTTGAGGGTTGCTAGTCT+TGG	0.451958	3:-1292114	MsaT011317.1:CDS
AAAATTATATTAGACGATGA+TGG	0.467412	3:-1290787	MsaT011317.1:CDS
CACCCTCACCTCGTTTGTTG+AGG	0.472935	3:-1290681	MsaT011317.1:CDS
TGTTATTGAACTCAGCAATC+CGG	0.481393	3:-1294876	MsaT011317.1:CDS
ACCAAGTTATTTGAGGAACT+TGG	0.485648	3:-1293580	MsaT011317.1:CDS
AGATTCGGTGGTGAAAGAGT+AGG	0.488495	3:+1292838	None:intergenic
AACCGAGTTCCTTTAGAAGC+AGG	0.491153	3:-1289983	MsaT011317.1:CDS
GCTAGTCTTGGAGTCATTGG+TGG	0.491588	3:-1292102	MsaT011317.1:CDS
CCTTTATTATGGAATACTGT+TGG	0.492405	3:-1293556	MsaT011317.1:CDS
CATCCTCTACCTGCTTCTAA+AGG	0.500981	3:+1289974	None:intergenic
GTTCCTTTAGAAGCAGGTAG+AGG	0.520910	3:-1289977	MsaT011317.1:CDS
TCCAAGTTCCTCAAATAACT+TGG	0.522228	3:+1293579	None:intergenic
TGGCATCTCACCCTGATACA+AGG	0.537570	3:-1292569	MsaT011317.1:CDS
AACACAGTTTGAGAATTTGA+GGG	0.539961	3:-1292127	MsaT011317.1:CDS
CTGTTGTTACATGAGAATGT+TGG	0.540471	3:-1290010	MsaT011317.1:CDS
AGTCATTGGTGGCTCGATGA+AGG	0.546457	3:-1292091	MsaT011317.1:intron
AGCCTCAACAAACGAGGTGA+GGG	0.559488	3:+1290679	None:intergenic
TGCTCTCTATGTTCTCTCCA+AGG	0.560738	3:-1294840	MsaT011317.1:intron
TGTTTGTCTAATATGCAAAA+CGG	0.565201	3:-1290955	MsaT011317.1:CDS
ACAAGGATCTCATTTCTCAA+TGG	0.566776	3:-1292552	MsaT011317.1:intron
AAGCCTCAACAAACGAGGTG+AGG	0.569309	3:+1290678	None:intergenic
GAAATGAGATCCTTGTATCA+GGG	0.596244	3:+1292559	None:intergenic
CACACTCGAGTTGATTGTGA+GGG	0.603583	3:+1292811	None:intergenic
TAACACCACTGATCATAGGA+TGG	0.625981	3:-1294915	MsaT011317.1:CDS
CTCAACAAACGAGGTGAGGG+TGG	0.629401	3:+1290682	None:intergenic
TACCTGCTTCTAAAGGAACT+CGG	0.631809	3:+1289981	None:intergenic
GATTGCTGAGTTCAATAACA+AGG	0.649486	3:+1294879	None:intergenic
ATCAAAAGCCTCAACAAACG+AGG	0.660997	3:+1290673	None:intergenic
GCACACTCGAGTTGATTGTG+AGG	0.665692	3:+1292810	None:intergenic
GTAAGCCATCCTATGATCAG+TGG	0.669763	3:+1294910	None:intergenic
TCAGTGGTGTTAGAATCACC+AGG	0.685443	3:+1294926	None:intergenic
TGTGAGGGAGAGAGATTCGG+TGG	0.689243	3:+1292826	None:intergenic
GTTGTTACATGAGAATGTTG+GGG	0.746517	3:-1290008	MsaT011317.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	1289973	1295008	1289973	ID=MsaG011317
Chr3	mRNA	1289973	1295008	1289973	ID=MsaT011317.1;Parent=MsaG011317
Chr3	exon	1289973	1290059	1289973	ID=MsaT011317.1.exon9;Parent=MsaT011317.1
Chr3	CDS	1289973	1290059	1289973	ID=cds.MsaT011317.1;Parent=MsaT011317.1
Chr3	exon	1290478	1290547	1290478	ID=MsaT011317.1.exon8;Parent=MsaT011317.1
Chr3	CDS	1290478	1290547	1290478	ID=cds.MsaT011317.1;Parent=MsaT011317.1
Chr3	exon	1290640	1290830	1290640	ID=MsaT011317.1.exon7;Parent=MsaT011317.1
Chr3	CDS	1290640	1290830	1290640	ID=cds.MsaT011317.1;Parent=MsaT011317.1
Chr3	exon	1290936	1291031	1290936	ID=MsaT011317.1.exon6;Parent=MsaT011317.1
Chr3	CDS	1290936	1291031	1290936	ID=cds.MsaT011317.1;Parent=MsaT011317.1
Chr3	exon	1292092	1292198	1292092	ID=MsaT011317.1.exon5;Parent=MsaT011317.1
Chr3	CDS	1292092	1292198	1292092	ID=cds.MsaT011317.1;Parent=MsaT011317.1
Chr3	exon	1292553	1292595	1292553	ID=MsaT011317.1.exon4;Parent=MsaT011317.1
Chr3	CDS	1292553	1292595	1292553	ID=cds.MsaT011317.1;Parent=MsaT011317.1
Chr3	exon	1292789	1292878	1292789	ID=MsaT011317.1.exon3;Parent=MsaT011317.1
Chr3	CDS	1292789	1292878	1292789	ID=cds.MsaT011317.1;Parent=MsaT011317.1
Chr3	exon	1293534	1293605	1293534	ID=MsaT011317.1.exon2;Parent=MsaT011317.1
Chr3	CDS	1293534	1293605	1293534	ID=cds.MsaT011317.1;Parent=MsaT011317.1
Chr3	exon	1294841	1295008	1294841	ID=MsaT011317.1.exon1;Parent=MsaT011317.1
Chr3	CDS	1294841	1295008	1294841	ID=cds.MsaT011317.1;Parent=MsaT011317.1

Gene Sequence

>MsaT011317.1

ATGGCACACTTTACTCCTCAATCGCTCTCAACAAAGAACAATGCATCGTTTATAGCTTTTCCTGGTGATTCTAACACCACTGATCATAGGATGGCTTACATGCAAAGCCTTGTTATTGAACTCAGCAATCCGGATCTTAGAGAAAATGCTCTCTATGTTCTCTCCAAGAGCACCAAGTTATTTGAGGAACTTGGACCTTTATTATGGAATACTGTTGGTACTATTGCAGTATTTTTACAGGAAATACTTTCAATATATCCTACTCTTTCACCACCGAATCTCTCTCCCTCACAATCAACTCGAGTGTGCAATGTTCTTGCACTACTTCAGTGTGTGGCATCTCACCCTGATACAAGGATCTCATTTCTCAATGCTAATATGCCTTTATATTTATATCCATTCCTTAAAACAACAAGCAAGTTAACACAGTTTGAGAATTTGAGGGTTGCTAGTCTTGGAGTCATTGGTGGCTCGATGAAGGTTAACACCAAAGAAGTCATAGGTTTCTATCTCTCAAGTGAGATAATTTCACTATGTTTGTCTAATATGCAAAACGGCAATGAATTGACAAAAAATGTTGCAACATTTATAATTCTGAAAATTATATTAGACGATGATGGTTTGGCTCGTGTTTGTGCTACAACAGAGAATTTTTTTGGAGTATGTCGAGTTTTGAACATGATGTTGGAAGATCTTGAGAATCCACCCTCACCTCGTTTGTTGAGGCTTTTGATTTCATGTTATTCTCGCTTATCTCAAAATTGCAGGGCTAGAGTTGCATTAGCAACTTATCTTCCAAACAAGCTCAAAGATGCCAATCTCATCAATTTTTTTCGTGATGATCCAAAAACTATGAGGTTGGTACTGTTGTTACATGAGAATGTTGGGGTGAACCGAGTTCCTTTAGAAGCAGGTAGAGGATGA

Protein sequence

>MsaT011317.1

MAHFTPQSLSTKNNASFIAFPGDSNTTDHRMAYMQSLVIELSNPDLRENALYVLSKSTKLFEELGPLLWNTVGTIAVFLQEILSIYPTLSPPNLSPSQSTRVCNVLALLQCVASHPDTRISFLNANMPLYLYPFLKTTSKLTQFENLRVASLGVIGGSMKVNTKEVIGFYLSSEIISLCLSNMQNGNELTKNVATFIILKIILDDDGLARVCATTENFFGVCRVLNMMLEDLENPPSPRLLRLLISCYSRLSQNCRARVALATYLPNKLKDANLINFFRDDPKTMRLVLLLHENVGVNRVPLEAGRG*