Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011364 | XP_003598704.1 | 92.840 | 824 | 55 | 2 | 1 | 821 | 1 | 823 | 0.0 | 1574 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011364 | sp|O81832|Y4729_ARATH | 46.514 | 832 | 385 | 9 | 1 | 821 | 1 | 783 | 0.0 | 748 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011364 | tr|G7IYY1|G7IYY1_MEDTR | 92.840 | 824 | 55 | 2 | 1 | 821 | 1 | 823 | 0.0 | 1574 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG003523 | MsaG011364 | 0.829039 | 1.152920e-54 | 3.246152e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011364 | MtrunA17_Chr3g0084451 | 92.840 | 824 | 55 | 2 | 1 | 821 | 1 | 823 | 0.0 | 1574 |
| MsaG011364 | MtrunA17_Chr3g0084441 | 90.073 | 826 | 74 | 4 | 1 | 821 | 1 | 823 | 0.0 | 1517 |
| MsaG011364 | MtrunA17_Chr3g0084491 | 85.280 | 822 | 108 | 5 | 1 | 821 | 14 | 823 | 0.0 | 1436 |
| MsaG011364 | MtrunA17_Chr3g0084411 | 81.159 | 828 | 141 | 7 | 1 | 821 | 1 | 820 | 0.0 | 1373 |
| MsaG011364 | MtrunA17_Chr3g0084691 | 79.693 | 847 | 135 | 5 | 1 | 821 | 1 | 836 | 0.0 | 1369 |
| MsaG011364 | MtrunA17_Chr3g0084431 | 81.598 | 826 | 131 | 7 | 1 | 821 | 1 | 810 | 0.0 | 1357 |
| MsaG011364 | MtrunA17_Chr3g0084421 | 83.463 | 774 | 117 | 5 | 55 | 821 | 14 | 783 | 0.0 | 1314 |
| MsaG011364 | MtrunA17_Chr3g0084681 | 75.820 | 823 | 151 | 4 | 1 | 821 | 1 | 777 | 0.0 | 1267 |
| MsaG011364 | MtrunA17_Chr3g0084621 | 81.450 | 593 | 105 | 2 | 1 | 591 | 1 | 590 | 0.0 | 983 |
| MsaG011364 | MtrunA17_Chr3g0098791 | 76.174 | 596 | 130 | 4 | 1 | 591 | 1 | 589 | 0.0 | 931 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011364 | AT4G27290.2 | 47.066 | 835 | 422 | 8 | 1 | 821 | 1 | 829 | 0.0 | 769 |
| MsaG011364 | AT4G27290.1 | 46.514 | 832 | 385 | 9 | 1 | 821 | 1 | 783 | 0.0 | 748 |
| MsaG011364 | AT4G21380.1 | 44.458 | 830 | 430 | 11 | 21 | 821 | 23 | 850 | 0.0 | 712 |
| MsaG011364 | AT4G27300.1 | 47.649 | 808 | 391 | 15 | 29 | 821 | 25 | 815 | 0.0 | 706 |
| MsaG011364 | AT1G65790.1 | 44.982 | 827 | 421 | 11 | 23 | 821 | 23 | 843 | 0.0 | 696 |
| MsaG011364 | AT1G65800.1 | 44.511 | 829 | 421 | 14 | 22 | 821 | 29 | 847 | 0.0 | 677 |
| MsaG011364 | AT1G11340.1 | 43.101 | 819 | 432 | 13 | 31 | 821 | 21 | 833 | 0.0 | 663 |
| MsaG011364 | AT1G11410.1 | 42.472 | 817 | 440 | 10 | 31 | 820 | 24 | 837 | 0.0 | 649 |
| MsaG011364 | AT1G11410.4 | 42.472 | 817 | 440 | 10 | 31 | 820 | 31 | 844 | 0.0 | 649 |
| MsaG011364 | AT1G11410.3 | 42.365 | 812 | 438 | 10 | 36 | 820 | 5 | 813 | 0.0 | 644 |
Find 166 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCACAGATGACATGTT+AGG | 0.562151 | 3:+1881905 | None:intergenic |
| AAAATATTACTAGGGCGTAA+TGG | 0.518488 | 3:-1883420 | MsaT011364.1:intron |
| AAACCAAAATCTGCTATCTT+TGG | 0.231728 | 3:+1882949 | None:intergenic |
| AAACGTTGTGAAGTTCCATA+TGG | 0.448875 | 3:+1884688 | None:intergenic |
| AAAGTAATCCAAGCTTCTGT+TGG | 0.546128 | 3:+1883226 | None:intergenic |
| AAATCAGTCTATGCAATACG+GGG | 0.709193 | 3:-1885376 | MsaT011364.1:CDS |
| AAATCTGATGTATTTAGCTT+TGG | 0.373124 | 3:-1882406 | MsaT011364.1:CDS |
| AACATCGGCACAAGGAACCG+AGG | 0.668089 | 3:-1883360 | MsaT011364.1:CDS |
| AACCAAAATCTGCTATCTTT+GGG | 0.378597 | 3:+1882950 | None:intergenic |
| AACCCGGGTTCATTTGGTTT+TGG | 0.229082 | 3:+1881857 | None:intergenic |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 1881763 | 1885499 | 1881763 | ID=MsaG011364 |
| Chr3 | mRNA | 1881763 | 1885499 | 1881763 | ID=MsaT011364.1;Parent=MsaG011364 |
| Chr3 | exon | 1881763 | 1882065 | 1881763 | ID=MsaT011364.1.exon7;Parent=MsaT011364.1 |
| Chr3 | CDS | 1881763 | 1882065 | 1881763 | ID=cds.MsaT011364.1;Parent=MsaT011364.1 |
| Chr3 | exon | 1882324 | 1882474 | 1882324 | ID=MsaT011364.1.exon6;Parent=MsaT011364.1 |
| Chr3 | CDS | 1882324 | 1882474 | 1882324 | ID=cds.MsaT011364.1;Parent=MsaT011364.1 |
| Chr3 | exon | 1882889 | 1883126 | 1882889 | ID=MsaT011364.1.exon5;Parent=MsaT011364.1 |
| Chr3 | CDS | 1882889 | 1883126 | 1882889 | ID=cds.MsaT011364.1;Parent=MsaT011364.1 |
| Chr3 | exon | 1883219 | 1883429 | 1883219 | ID=MsaT011364.1.exon4;Parent=MsaT011364.1 |
| Chr3 | CDS | 1883219 | 1883429 | 1883219 | ID=cds.MsaT011364.1;Parent=MsaT011364.1 |
| Chr3 | exon | 1883516 | 1883670 | 1883516 | ID=MsaT011364.1.exon3;Parent=MsaT011364.1 |
| Chr3 | CDS | 1883516 | 1883670 | 1883516 | ID=cds.MsaT011364.1;Parent=MsaT011364.1 |
| Chr3 | exon | 1883828 | 1883953 | 1883828 | ID=MsaT011364.1.exon2;Parent=MsaT011364.1 |
| Chr3 | CDS | 1883828 | 1883953 | 1883828 | ID=cds.MsaT011364.1;Parent=MsaT011364.1 |
| Chr3 | exon | 1884218 | 1885499 | 1884218 | ID=MsaT011364.1.exon1;Parent=MsaT011364.1 |
| Chr3 | CDS | 1884218 | 1885499 | 1884218 | ID=cds.MsaT011364.1;Parent=MsaT011364.1 |
| Gene Sequence |
>MsaT011364.1 ATGGAGAACAATAACAAGGGGATTATGCTAATGGTGTTTTTTTTCTTCTTTTGCTCCTTGCCCAATTTCTCTATACAGAAAACTTTTACTACTATTGCTCCAAATCAGTCTATGCAATACGGGGATACGCTTGTTTCTGCAGCTGAGAGGTATGAAGCAGGATTTTTCAACTTTGGCGATCCACAACGTCAATACTTCGGTATATGGTACAAGAACATATCGCCGAGGACTATTGTGTGGGTTGCCAATAGAAATACTCCTGTACATAACTCAACAGAAATGCTGAAACTCACCGATCAAGGAAGTCTTGTTATTCTTGATGGCTCCAAAGGCATTATCTGGTCCTCCAATTCATCAAGAACTGTGGTGAAATCAGTTGTTCAGCTTTTGGATTCGGGAAATCTTGTCCTGAAGGATGCAAACAACTCTCAGAACTTTCTGTGGGAAAGTTTTGACTATCCTGGTAACACTTTCCTTGCTGGAATGAAGCTGAAAAGTAATTTAGTTACTGGTCCATATAGATATCTCACATCTTGGAAAAGCCCTCAAGATCCGGCTGAAGGTGAGTGTTCATATTGGATAGATATGCATGGTTTTCCTCAACTGGTTACTGTAAAGGGATCAACAGTTCTGTATAGAGGAGGGTCATGGAATGGATTTCTTTTCACTGGTGTTTCTTGGCAAAGACTGCATAGAGTCTTGAATTTCTCAGTAGTGGTCACCGACAAAGAATTCTCTTATCAATATCAAACTTTGAACAGTTCAATTAATACTAGATTGGTGCTAGATCCATATGGAACTTCACAACGTTTTCAATGGTCAGATAGGACACAGATTTGGGAGGCTATATATGCTCTTCCTTCAGACCAGTGTGATGCTTATGACTTATGTGGTAACAATTCTAACTGCAATGGTGATATCTATCCAATATGTGAATGCTTAGAAGGTTTCATGCCAAAATCTCAACCAGAGTGGGAGTCATCAAATTGGTCTGGTGGGTGTGCAAGGAAAACACGTTTAAATTGTCTCCATGGCGATGGATTTTTACCGTACACAAACATGAAGTTGCCAGACACGTCAACATCTTGGTATAACAAGAGCTTGAGCCTTGAGGAATGTAAGACAAGGTGTTTGAAAAACTGTTCTTGCACTGCATATGCAAATTCAGATATCAGAGATGGTGGGAGTGGCTGCTTACTTTGGTTTGACAACATTGTGGACATGAGAAAACATCAAGACCAAGGTCAAGACATTTACATACGGCTGGCATCTTCAGAACTTGATCATAAAAAGAACAAGCGGAAATTGAAGCTTGCTGGGACTCTTGCAGGAGTTGTTGCATTCATTATAGGACTAACCGTTCTCGTATTGATCACATCAGCATATAGAAAGAACCTTGGTAAGCCGAGGTATATAAAAAAGTTATTTCTCTGGAAGCACAAGAAGGAGAGGGAATATGGTGATTTGGCAACAATATTTGATTTTTCAACCATCACTATTGCAACAAATAATTTCTCTGTCAAAAACAAGTTGGGAGAAGGTGGTTTTGGATCAGTGTACAAGGGCGTAATGGTAGACGGCCAAGAGATTGCTGTTAAGAGGCTTTCTAAAACATCGGCACAAGGAACCGAGGAGTTCAAAAATGAAGTAAACTTGATGGCAACACTTCAACACCGTAATCTTGTCAAACTTCTTGGTTGTTCTATTCAACAAGATGAAAAGTTGTTGATCTATGAATTCATGGCCAACAGAAGCTTGGATTACTTTATTTTTGATACTATGCGAAGCAAATTGCTAAATTGGAATAAGCGCCTGGAAATTATTGATGGAATTGCTCGAGGGCTGTTGTATCTTCATCAAGATTCTACACTAAGAATCATACATAGAGATATGAAAACAAGCAATATTCTTCTTGATATTGATATGATCCCAAAGATAGCAGATTTTGGTTTAGCCAGATCATTTATGGGAGACGAAGCAGAGGCTAATACAAATAGATTGATCGGATCATATGGATATATGCCTCCAGAATATGCAGCGGATGGATCCTTCTCAATAAAATCTGATGTATTTAGCTTTGGAGTCGTTGTACTTGAGATAATTAGCGGTAGAAAGAATCACGGATTTCGTGACCCTCTACATCGTCTTAACCTTCTTGGTCATGCATGGAGACTTTGGATTGAAGGAAGGCCACTGGATTTAATAGCTGACATATTATATGATGATGAGGCCATATGTTCAGAAATAATAAGATTTATTCATGTTGGTCTATTGTGTGTTCAACAGCTACCAGAAAATAGGCCTAACATGTCATCTGTGGTTTTTATGTTAAAGGGTGAAAAATTACTCCCAAAACCAAATGAACCCGGGTTCTACACTGCAAGGGATAATACAAATAGCATAGAATCTTCATCTAAAGAGTTTTCAATAAATGAAGCTTCAATCTCATTGTTAGACGCCCGGTAG |
| Protein sequence |
>MsaT011364.1 MENNNKGIMLMVFFFFFCSLPNFSIQKTFTTIAPNQSMQYGDTLVSAAERYEAGFFNFGDPQRQYFGIWYKNISPRTIVWVANRNTPVHNSTEMLKLTDQGSLVILDGSKGIIWSSNSSRTVVKSVVQLLDSGNLVLKDANNSQNFLWESFDYPGNTFLAGMKLKSNLVTGPYRYLTSWKSPQDPAEGECSYWIDMHGFPQLVTVKGSTVLYRGGSWNGFLFTGVSWQRLHRVLNFSVVVTDKEFSYQYQTLNSSINTRLVLDPYGTSQRFQWSDRTQIWEAIYALPSDQCDAYDLCGNNSNCNGDIYPICECLEGFMPKSQPEWESSNWSGGCARKTRLNCLHGDGFLPYTNMKLPDTSTSWYNKSLSLEECKTRCLKNCSCTAYANSDIRDGGSGCLLWFDNIVDMRKHQDQGQDIYIRLASSELDHKKNKRKLKLAGTLAGVVAFIIGLTVLVLITSAYRKNLGKPRYIKKLFLWKHKKEREYGDLATIFDFSTITIATNNFSVKNKLGEGGFGSVYKGVMVDGQEIAVKRLSKTSAQGTEEFKNEVNLMATLQHRNLVKLLGCSIQQDEKLLIYEFMANRSLDYFIFDTMRSKLLNWNKRLEIIDGIARGLLYLHQDSTLRIIHRDMKTSNILLDIDMIPKIADFGLARSFMGDEAEANTNRLIGSYGYMPPEYAADGSFSIKSDVFSFGVVVLEIISGRKNHGFRDPLHRLNLLGHAWRLWIEGRPLDLIADILYDDEAICSEIIRFIHVGLLCVQQLPENRPNMSSVVFMLKGEKLLPKPNEPGFYTARDNTNSIESSSKEFSINEASISLLDAR* |
