AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011473


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011473 MtrunA17_Chr3g0085681 97.011 435 13 0 9 443 4 438 0.0 880
MsaG011473 MtrunA17_Chr3g0085361 95.677 347 14 1 97 443 7 352 0.0 685
MsaG011473 MtrunA17_Chr5g0396491 54.788 449 191 5 7 443 32 480 1.27e-169 485
MsaG011473 MtrunA17_Chr4g0038851 50.112 447 213 4 7 443 25 471 1.32e-159 459
MsaG011473 MtrunA17_Chr5g0447691 30.788 419 230 6 31 441 178 544 1.15e-61 209
MsaG011473 MtrunA17_Chr1g0159461 29.976 417 236 4 31 441 226 592 1.29e-59 204
MsaG011473 MtrunA17_Chr8g0376381 29.787 423 217 10 31 442 170 523 4.08e-59 202
MsaG011473 MtrunA17_Chr2g0319091 30.935 417 230 7 31 440 213 578 3.02e-56 195
MsaG011473 MtrunA17_Chr5g0445331 27.685 419 247 6 31 441 162 532 2.03e-55 192
MsaG011473 MtrunA17_Chr3g0125811 28.302 424 248 4 24 441 197 570 6.37e-55 191
MsaG011473 MtrunA17_Chr7g0224271 29.128 436 221 8 31 441 301 673 1.78e-53 189
MsaG011473 MtrunA17_Chr7g0245601 29.398 415 224 8 33 441 441 792 2.67e-53 191
MsaG011473 MtrunA17_Chr3g0101141 27.818 417 245 6 31 441 176 542 3.10e-53 186
MsaG011473 MtrunA17_Chr2g0306641 30.238 420 226 9 31 441 126 487 4.40e-53 185
MsaG011473 MtrunA17_Chr4g0076091 26.841 421 243 7 31 440 176 542 1.21e-47 171
MsaG011473 MtrunA17_Chr4g0057291 27.078 421 244 8 31 440 293 661 2.24e-46 170
MsaG011473 MtrunA17_Chr2g0290241 26.027 438 258 8 19 441 39 425 5.43e-46 164
MsaG011473 MtrunA17_Chr2g0328391 26.555 418 250 6 31 440 300 668 1.40e-45 167
MsaG011473 MtrunA17_Chr8g0357601 27.934 426 236 9 31 441 183 552 6.79e-45 164
MsaG011473 MtrunA17_Chr2g0328411 28.469 418 237 8 31 440 290 653 7.01e-45 166
MsaG011473 MtrunA17_Chr4g0031721 27.990 418 242 7 31 440 317 683 9.25e-45 165
MsaG011473 MtrunA17_Chr4g0031741 28.708 418 237 8 31 440 285 649 1.80e-43 161
MsaG011473 MtrunA17_Chr4g0031711 27.423 423 242 9 31 440 362 732 1.79e-42 159
MsaG011473 MtrunA17_Chr7g0238891 27.273 418 247 6 31 441 373 740 2.66e-42 159
MsaG011473 MtrunA17_Chr2g0328431 27.818 417 241 7 31 440 290 653 7.07e-42 157
MsaG011473 MtrunA17_Chr2g0328341 26.969 419 243 8 31 440 273 637 9.38e-42 156
MsaG011473 MtrunA17_Chr2g0328381 25.542 415 254 7 31 440 276 640 1.39e-41 156
MsaG011473 MtrunA17_Chr4g0057891 26.241 423 248 7 31 441 150 520 2.53e-41 154
MsaG011473 MtrunA17_Chr4g0031701 26.316 418 245 9 31 436 255 621 1.37e-40 153
MsaG011473 MtrunA17_Chr4g0038761 29.126 412 233 10 33 441 87 442 3.45e-40 149
MsaG011473 MtrunA17_Chr4g0031731 26.139 417 247 8 31 436 255 621 5.32e-40 151
MsaG011473 MtrunA17_Chr1g0183641 26.291 426 247 10 23 443 116 479 6.17e-37 141
MsaG011473 MtrunA17_Chr2g0294471 28.037 428 237 9 31 442 205 577 2.28e-36 140
MsaG011473 MtrunA17_Chr4g0039661 27.103 428 240 10 26 441 188 555 3.86e-36 140
MsaG011473 MtrunA17_Chr2g0328401 26.854 391 227 6 31 415 291 628 3.96e-36 140
MsaG011473 MtrunA17_Chr2g0294461 27.506 429 241 8 31 442 211 586 1.16e-35 139
MsaG011473 MtrunA17_Chr2g0328361 25.225 444 244 11 6 440 250 614 2.56e-32 129
MsaG011473 MtrunA17_Chr5g0401131 23.349 424 264 8 31 441 115 490 2.74e-32 128
MsaG011473 MtrunA17_Chr5g0424131 24.190 401 236 7 56 440 150 498 3.40e-29 119
MsaG011473 MtrunA17_Chr3g0114841 24.215 446 256 13 21 440 110 499 1.88e-28 117
MsaG011473 MtrunA17_Chr1g0200401 24.289 457 257 15 15 441 62 459 1.95e-28 117
MsaG011473 MtrunA17_Chr3g0087711 87.931 58 7 0 181 238 1 58 2.40e-28 106
MsaG011473 MtrunA17_Chr3g0110971 39.752 161 83 3 296 442 428 588 3.21e-28 117
MsaG011473 MtrunA17_Chr3g0110971 30.144 209 132 3 31 234 220 419 7.31e-28 116
MsaG011473 MtrunA17_Chr4g0053901 23.982 442 270 10 31 440 60 467 2.23e-26 110
MsaG011473 MtrunA17_Chr2g0328371 25.704 284 159 5 112 392 12 246 3.47e-26 106
MsaG011473 MtrunA17_Chr7g0236851 25.714 420 244 12 31 440 295 656 1.47e-24 106
MsaG011473 MtrunA17_Chr5g0401421 22.864 433 288 11 31 440 36 445 4.42e-24 103
MsaG011473 MtrunA17_Chr7g0270031 23.485 396 237 9 57 442 216 555 9.10e-24 103
MsaG011473 MtrunA17_Chr1g0194621 24.561 399 226 13 61 442 179 519 1.33e-19 91.3
MsaG011473 MtrunA17_Chr6g0468561 24.471 425 249 16 31 441 296 662 3.68e-19 90.1
MsaG011473 MtrunA17_Chr2g0329591 22.529 435 255 11 31 441 75 451 2.14e-18 87.0
MsaG011473 MtrunA17_Chr4g0012071 22.115 416 258 11 31 441 197 551 1.29e-17 85.1
MsaG011473 MtrunA17_Chr8g0383981 23.038 395 229 12 57 440 165 495 1.58e-17 84.7
MsaG011473 MtrunA17_Chr1g0207361 47.525 101 50 2 249 346 64 164 6.55e-17 78.2
MsaG011473 MtrunA17_Chr2g0323111 29.452 146 102 1 295 440 161 305 2.24e-16 79.7
MsaG011473 MtrunA17_Chr7g0230771 31.915 141 94 1 31 171 376 514 1.87e-11 66.2
MsaG011473 MtrunA17_Chr7g0230731 31.915 141 94 1 31 171 373 511 2.13e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011473 AT1G50420.1 49.330 448 216 4 7 443 34 481 5.93e-151 437
MsaG011473 AT1G66350.1 31.075 428 225 10 21 442 144 507 4.04e-63 212
MsaG011473 AT5G48150.1 30.324 432 237 5 16 441 117 490 5.49e-62 209
MsaG011473 AT5G48150.4 30.324 432 237 5 16 441 117 490 5.49e-62 209
MsaG011473 AT5G48150.2 30.324 432 237 5 16 441 117 490 5.49e-62 209
MsaG011473 AT5G48150.3 30.324 432 237 5 16 441 117 490 5.49e-62 209
MsaG011473 AT3G03450.1 29.009 424 228 9 31 442 184 546 8.68e-59 202
MsaG011473 AT1G50600.3 29.496 417 238 4 31 441 160 526 7.65e-58 199
MsaG011473 AT1G50600.2 29.496 417 238 4 31 441 235 601 2.13e-57 199
MsaG011473 AT1G50600.1 29.496 417 238 4 31 441 231 597 2.16e-57 199
MsaG011473 AT2G04890.1 30.583 412 233 7 31 441 54 413 3.09e-57 194
MsaG011473 AT2G01570.1 28.504 421 229 8 31 442 225 582 1.66e-54 191
MsaG011473 AT1G14920.1 29.812 426 217 11 31 442 173 530 2.43e-54 189
MsaG011473 AT4G17230.1 28.297 417 243 4 31 441 159 525 4.50e-52 183
MsaG011473 AT4G17230.2 28.297 417 243 4 31 441 159 525 4.50e-52 183
MsaG011473 AT3G54220.1 28.744 414 235 7 33 443 296 652 8.75e-52 185
MsaG011473 AT1G21450.1 29.009 424 244 7 25 441 220 593 1.08e-50 181
MsaG011473 AT1G63100.1 25.059 427 252 5 31 440 253 628 6.14e-45 166
MsaG011473 AT1G63100.2 25.059 427 252 5 31 440 278 653 7.94e-45 166
MsaG011473 AT2G29060.2 28.741 421 238 10 31 440 285 654 9.81e-44 162
MsaG011473 AT2G29060.1 28.741 421 238 10 31 440 322 691 1.44e-43 162
MsaG011473 AT1G07530.1 27.316 421 242 9 31 440 397 764 6.62e-43 161
MsaG011473 AT5G59450.1 28.504 421 241 8 31 441 228 598 4.56e-42 157
MsaG011473 AT5G41920.1 27.208 419 235 8 31 441 44 400 2.11e-41 152
MsaG011473 AT3G46600.2 27.990 418 243 8 31 441 5 371 1.59e-39 146
MsaG011473 AT3G46600.3 27.880 434 251 9 19 441 165 547 1.25e-38 147
MsaG011473 AT3G46600.1 27.880 434 251 9 19 441 197 579 3.40e-38 146
MsaG011473 AT1G07520.2 25.175 429 247 9 31 440 155 528 4.85e-36 139
MsaG011473 AT1G07520.4 25.175 429 247 9 31 440 155 528 4.85e-36 139
MsaG011473 AT1G55580.1 24.945 453 246 7 31 441 45 445 1.60e-35 137
MsaG011473 AT1G07520.3 25.175 429 247 9 31 440 320 693 1.86e-35 139
MsaG011473 AT1G07520.1 25.175 429 247 9 31 440 319 692 1.90e-35 139
MsaG011473 AT2G37650.1 25.837 418 251 7 31 441 348 713 4.78e-35 138
MsaG011473 AT5G66770.1 27.187 423 237 9 31 441 221 584 5.41e-35 137
MsaG011473 AT2G37650.2 25.837 418 251 7 31 441 336 701 5.65e-35 138
MsaG011473 AT2G29065.1 24.590 427 253 8 31 440 253 627 2.28e-32 130
MsaG011473 AT3G50650.1 25.743 404 226 7 53 441 198 542 3.95e-32 129
MsaG011473 AT4G37650.1 23.112 437 256 12 31 441 147 529 7.95e-32 127
MsaG011473 AT5G52510.1 23.843 432 248 9 31 441 269 640 7.45e-30 122
MsaG011473 AT5G17490.1 30.769 208 133 5 31 234 161 361 4.71e-29 119
MsaG011473 AT5G17490.1 38.000 150 90 2 295 442 369 517 7.34e-26 110
MsaG011473 AT3G49950.1 22.512 422 270 12 31 440 31 407 1.16e-24 105
MsaG011473 AT4G08250.1 22.603 438 271 12 17 440 93 476 3.56e-23 102

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAAGTTTGCTAATTAT+TGG 0.118308 3:+4291943 None:intergenic
CTGTATCGGCCTGGAAATTT+AGG 0.184893 3:-4291234 MsaT011473.1:CDS
GACAACCGAAGCAGGGATTT+TGG 0.226524 3:-4291986 MsaT011473.1:CDS
TCTGAATGGATGTAATTTAA+TGG 0.250687 3:-4291596 MsaT011473.1:CDS
ATGGATTCAAAGACTTAAAA+TGG 0.281483 3:-4291395 MsaT011473.1:CDS
AAACTCGAGAGCATGCTTCT+TGG 0.323417 3:-4291487 MsaT011473.1:CDS
TTAAGAAAGATCCCCATCTT+AGG 0.362039 3:+4291673 None:intergenic
TAATTGCTTGATTTGTGATC+AGG 0.367971 3:+4292184 None:intergenic
CCGCCCCATCTGAAAATTAC+AGG 0.369403 3:-4292054 MsaT011473.1:CDS
TTTAGGTGCTTCACCTAAGA+TGG 0.374066 3:-4291686 MsaT011473.1:CDS
ATTATTGGATTGAACTGTAA+TGG 0.377503 3:+4291958 None:intergenic
ATGATTGTTTACTTGTTTGT+TGG 0.377997 3:-4291276 MsaT011473.1:CDS
AAGAGGTGATAATGCTGAAT+CGG 0.390322 3:+4291711 None:intergenic
GAATCATCACCTAAATTTCC+AGG 0.414889 3:+4291225 None:intergenic
AATTCCCTCAGCCAGTGATA+TGG 0.418979 3:+4292510 None:intergenic
CGAATCGATGGAACGTGAAA+AGG 0.422571 3:-4292160 MsaT011473.1:CDS
TCTTGCCACTGATGATGAAA+TGG 0.424264 3:-4291845 MsaT011473.1:CDS
CACTCAATAAGAGGGATCAT+TGG 0.426274 3:+4292462 None:intergenic
TTCAGATGGGGCGGACCTCC+CGG 0.437773 3:+4292063 None:intergenic
ATTCAAAGACTTAAAATGGC+TGG 0.439982 3:-4291391 MsaT011473.1:CDS
TCAGTAATCACTAAAAGTTT+CGG 0.445565 3:+4291631 None:intergenic
TTTCATTTGACAACCGAAGC+AGG 0.447617 3:-4291994 MsaT011473.1:CDS
ACAAACTTTAAAGAAACGTC+CGG 0.481008 3:-4292082 MsaT011473.1:CDS
TAAAATGGCTGGATTTATGA+AGG 0.487865 3:-4291380 MsaT011473.1:CDS
TCATGAAAAGAAAGAAGTAT+TGG 0.489286 3:-4292028 MsaT011473.1:CDS
TGTGCAAAATGTGTTGCATC+TGG 0.490537 3:-4292441 MsaT011473.1:CDS
AGAGGTGATAATGCTGAATC+GGG 0.492776 3:+4291712 None:intergenic
TTAGGTGCTTCACCTAAGAT+GGG 0.494167 3:-4291685 MsaT011473.1:CDS
CACTAGGTTCGCCATATCAC+TGG 0.495093 3:-4292521 MsaT011473.1:CDS
GAAAACTTGCCTGTCAAGAC+AGG 0.495316 3:-4291910 MsaT011473.1:CDS
AAAATCCAAAATCCCTGCTT+CGG 0.496790 3:+4291981 None:intergenic
TGGTAATGGTGTACAAAGAA+TGG 0.500702 3:-4292361 MsaT011473.1:CDS
GAACAGGAATCAAATCTGAA+TGG 0.501626 3:-4291610 MsaT011473.1:CDS
ATTTATCCATTGCGCTGGCT+CGG 0.503400 3:+4292110 None:intergenic
CAACTCAGCTGTTATGCACT+AGG 0.503968 3:-4292537 MsaT011473.1:CDS
ATTTCATCACCTCATGGTAA+TGG 0.506040 3:-4292375 MsaT011473.1:CDS
ACAGCGTCTCCTGTCTTGAC+AGG 0.513933 3:+4291901 None:intergenic
AGGTACCTTTGAGTTACAAT+GGG 0.518881 3:-4291360 MsaT011473.1:CDS
ACTTATTTCAGTGAAGCACT+TGG 0.520928 3:-4292336 MsaT011473.1:CDS
TCAAGCAATTATCGAATCGA+TGG 0.531832 3:-4292172 MsaT011473.1:CDS
GATGAAATGGTCTCCTCAGC+AGG 0.533946 3:-4291832 MsaT011473.1:CDS
ATCAGATTTATCCATTGCGC+TGG 0.534751 3:+4292105 None:intergenic
TTGTGAGGGAGTCGATAGAA+AGG 0.541472 3:-4291440 MsaT011473.1:CDS
AAGGTACCTTTGAGTTACAA+TGG 0.544427 3:-4291361 MsaT011473.1:CDS
ATTGCGCTGGCTCGGAACAA+TGG 0.551742 3:+4292118 None:intergenic
TTTAATGGAGAGAATCGATC+GGG 0.556728 3:-4291581 MsaT011473.1:CDS
TCTCAGATTTCATCACCTCA+TGG 0.558265 3:-4292381 MsaT011473.1:CDS
TTCATTTGACAACCGAAGCA+GGG 0.560755 3:-4291993 MsaT011473.1:CDS
GAGCAGCGCACATGGGTCAG+AGG 0.566671 3:-4291786 MsaT011473.1:CDS
TACCTTTGAGTTACAATGGG+AGG 0.570596 3:-4291357 MsaT011473.1:CDS
GGCATGAGAAGCTCGAGCAA+TGG 0.571455 3:-4291414 MsaT011473.1:CDS
AAGAACATCATTACTTGTGA+GGG 0.575874 3:-4291454 MsaT011473.1:CDS
ATCCTCCCATTGTAACTCAA+AGG 0.576632 3:+4291355 None:intergenic
TATGCAGAGAGCAGCGCACA+TGG 0.577233 3:-4291794 MsaT011473.1:CDS
AAAGAACATCATTACTTGTG+AGG 0.578365 3:-4291455 MsaT011473.1:CDS
GATAGATGTCGCACCTGCTG+AGG 0.593900 3:+4291819 None:intergenic
ATCGATGGAACGTGAAAAGG+TGG 0.597890 3:-4292157 MsaT011473.1:CDS
GTTCGCCATATCACTGGCTG+AGG 0.598645 3:-4292515 MsaT011473.1:CDS
TTGAGAGCTTTATATACACC+AGG 0.605500 3:+4292291 None:intergenic
TAGGTGCTTCACCTAAGATG+GGG 0.607732 3:-4291684 MsaT011473.1:CDS
ATTTAATGGAGAGAATCGAT+CGG 0.609880 3:-4291582 MsaT011473.1:CDS
ATTGTTCCGAGCCAGCGCAA+TGG 0.617587 3:-4292116 MsaT011473.1:CDS
AAGATAGTTAAACATTTACC+TGG 0.625266 3:-4292309 MsaT011473.1:CDS
CAAACTTTAAAGAAACGTCC+GGG 0.635686 3:-4292081 MsaT011473.1:CDS
GGAGACCATTTCATCATCAG+TGG 0.644820 3:+4291840 None:intergenic
ATGCAGAGAGCAGCGCACAT+GGG 0.647261 3:-4291793 MsaT011473.1:CDS
GTCAGAGGACTTTCGCAGAG+TGG 0.652841 3:-4291771 MsaT011473.1:CDS
TTCGCCATATCACTGGCTGA+GGG 0.658373 3:-4292514 MsaT011473.1:CDS
AGGGAGTCGATAGAAAGGAA+AGG 0.670114 3:-4291435 MsaT011473.1:CDS
CTTTGTACACCATTACCATG+AGG 0.671867 3:+4292366 None:intergenic
ACTTTAAAGAAACGTCCGGG+AGG 0.713179 3:-4292078 MsaT011473.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 4291231 4292562 4291231 ID=MsaG011473
Chr3 mRNA 4291231 4292562 4291231 ID=MsaT011473.1;Parent=MsaG011473
Chr3 exon 4291231 4292562 4291231 ID=MsaT011473.1.exon1;Parent=MsaT011473.1
Chr3 CDS 4291231 4292562 4291231 ID=cds.MsaT011473.1;Parent=MsaT011473.1
Gene Sequence

>MsaT011473.1

ATGCAACTCAGCTGTTATGCACTAGGTTCGCCATATCACTGGCTGAGGGAATTGAGATATGACTCTCAGAGCTCAAATCCAATGATCCCTCTTATTGAGTGTGCAAAATGTGTTGCATCTGGAAGCATAAAAAATGCTGATATTGGACTTGAGTATATTTCTCAGATTTCATCACCTCATGGTAATGGTGTACAAAGAATGGTAACTTATTTCAGTGAAGCACTTGGATACAAGATAGTTAAACATTTACCTGGTGTATATAAAGCTCTCAATTCCTCGAAAATATCATCGTCTTCAGATGATATCCTTGTTCAAAAATACTTCTATGACCTGTGTCCTTTTTTGAAGTTTTCATACCTGATCACAAATCAAGCAATTATCGAATCGATGGAACGTGAAAAGGTGGTTCATATTATCGATCTCCATTGTTCCGAGCCAGCGCAATGGATAAATCTGATACAAACTTTAAAGAAACGTCCGGGAGGTCCGCCCCATCTGAAAATTACAGGAATTCATGAAAAGAAAGAAGTATTGGAGCAAATGAGCTTTCATTTGACAACCGAAGCAGGGATTTTGGATTTTCCATTACAGTTCAATCCAATAATTAGCAAACTTGAAGATGTCGATTTCGAAAACTTGCCTGTCAAGACAGGAGACGCTGTCGCAATTTCTTCTGTTCTTCAGCTGCATTCTCTTCTTGCCACTGATGATGAAATGGTCTCCTCAGCAGGTGCGACATCTATCAATATGCAGAGAGCAGCGCACATGGGTCAGAGGACTTTCGCAGAGTGGCTAGAGAGAGACATGATCAATGCATATATCTTGAGTCCCGATTCAGCATTATCACCTCTTTTTTTAGGTGCTTCACCTAAGATGGGGATCTTTCTTAACGCTATGCGAAAATTACAACCGAAACTTTTAGTGATTACTGAACAGGAATCAAATCTGAATGGATGTAATTTAATGGAGAGAATCGATCGGGCATTGTATTTTTACGGTTCACTTTTTGACTGCTTGGAATCTACTGTTACGAGAACATCAGTCGAGAGACAAAAACTCGAGAGCATGCTTCTTGGAGAGCAAATAAAGAACATCATTACTTGTGAGGGAGTCGATAGAAAGGAAAGGCATGAGAAGCTCGAGCAATGGATTCAAAGACTTAAAATGGCTGGATTTATGAAGGTACCTTTGAGTTACAATGGGAGGATAGAAGCGACAAATCTATTGCAGCGATATAGTCATAAATACAAGTTTAAAGAAGAAAATGATTGTTTACTTGTTTGTTGGAGTGACAGACCACTTTTTTCTGTATCGGCCTGGAAATTTAGGTGA

Protein sequence

>MsaT011473.1

MQLSCYALGSPYHWLRELRYDSQSSNPMIPLIECAKCVASGSIKNADIGLEYISQISSPHGNGVQRMVTYFSEALGYKIVKHLPGVYKALNSSKISSSSDDILVQKYFYDLCPFLKFSYLITNQAIIESMEREKVVHIIDLHCSEPAQWINLIQTLKKRPGGPPHLKITGIHEKKEVLEQMSFHLTTEAGILDFPLQFNPIISKLEDVDFENLPVKTGDAVAISSVLQLHSLLATDDEMVSSAGATSINMQRAAHMGQRTFAEWLERDMINAYILSPDSALSPLFLGASPKMGIFLNAMRKLQPKLLVITEQESNLNGCNLMERIDRALYFYGSLFDCLESTVTRTSVERQKLESMLLGEQIKNIITCEGVDRKERHEKLEQWIQRLKMAGFMKVPLSYNGRIEATNLLQRYSHKYKFKEENDCLLVCWSDRPLFSVSAWKFR*