Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011546 | TKY60965.1 | 67.200 | 125 | 33 | 2 | 5 | 123 | 206 | 328 | 3.16e-44 | 158 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011546 | tr|A0A1S3UWR7|A0A1S3UWR7_VIGRR | 70.000 | 120 | 32 | 2 | 5 | 120 | 226 | 345 | 1.82e-43 | 155 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG011546 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG011546 | MtrunA17_Chr1g0161031 | 60.417 | 96 | 36 | 1 | 30 | 123 | 228 | 323 | 3.57e-25 | 97.8 |
| MsaG011546 | MtrunA17_Chr6g0478581 | 38.554 | 83 | 45 | 1 | 47 | 123 | 299 | 381 | 4.31e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATGGCATCGATTACTTT+TGG | 0.271813 | 3:-5730821 | None:intergenic |
| GGAGGCTCGGCATTGCTAAC+AGG | 0.367844 | 3:-5730800 | None:intergenic |
| TTTCTCGCAGTTAAAGCAAC+TGG | 0.436705 | 3:+5730764 | MsaT011546.1:CDS |
| GCCATTACCATGCTGGTGTC+AGG | 0.443156 | 3:+5730836 | MsaT011546.1:CDS |
| GAGGAGGGTGGTGCATTTGA+AGG | 0.446466 | 3:-5730665 | None:intergenic |
| CACAGCACACAGAAAAGTTC+AGG | 0.446504 | 3:+5730613 | MsaT011546.1:intron |
| TTGTTAATTCAAAGGTGGAC+TGG | 0.471778 | 3:+5731485 | MsaT011546.1:CDS |
| TCGCATACTGGTGCACAGTC+AGG | 0.473936 | 3:+5730716 | MsaT011546.1:CDS |
| GTATCTATTTCATCGCATAC+TGG | 0.486436 | 3:+5730704 | MsaT011546.1:CDS |
| TCAAATGCACCACCCTCCTC+TGG | 0.491435 | 3:+5730668 | MsaT011546.1:CDS |
| TCAGGCTCGGAAAGCATCCT+TGG | 0.496291 | 3:+5731015 | MsaT011546.1:CDS |
| ACCTGACACCAGCATGGTAA+TGG | 0.513597 | 3:-5730837 | None:intergenic |
| GCATTTGAAGGTTGACTCAC+GGG | 0.518410 | 3:-5730653 | None:intergenic |
| CACAGAAAAGTTCAGGCACC+TGG | 0.524123 | 3:+5730620 | MsaT011546.1:CDS |
| CTCACGGGCACACCGTCACC+AGG | 0.540218 | 3:-5730638 | None:intergenic |
| GGCATTGCTAACAGGAGTGG+TGG | 0.550160 | 3:-5730792 | None:intergenic |
| TGCATTTGAAGGTTGACTCA+CGG | 0.556932 | 3:-5730654 | None:intergenic |
| ATGCAGCGATACCTCAGGCT+CGG | 0.560553 | 3:+5731002 | MsaT011546.1:intron |
| AATCGATGCCATTACCATGC+TGG | 0.562603 | 3:+5730829 | MsaT011546.1:CDS |
| GTATGCGATGAAATAGATAC+AGG | 0.564795 | 3:-5730701 | None:intergenic |
| ACTGGTGCACAGTCAGGCAG+TGG | 0.578273 | 3:+5730722 | MsaT011546.1:CDS |
| AAAGTTCAGGCACCTGGTGA+CGG | 0.580555 | 3:+5730626 | MsaT011546.1:CDS |
| TTACTTTGTTAATTCAAAGG+TGG | 0.582156 | 3:+5731480 | MsaT011546.1:CDS |
| GGAAAACATATATTTGTCGA+CGG | 0.616215 | 3:-5730074 | None:intergenic |
| GATGAAATAGATACAGGACT+TGG | 0.619496 | 3:-5730695 | None:intergenic |
| CTCGGCATTGCTAACAGGAG+TGG | 0.620116 | 3:-5730795 | None:intergenic |
| GCATTGCTAACAGGAGTGGT+GGG | 0.638959 | 3:-5730791 | None:intergenic |
| AAGGATGCTTTCCGAGCCTG+AGG | 0.641286 | 3:-5731013 | None:intergenic |
| AGGACTTGGATGACCAGAGG+AGG | 0.643414 | 3:-5730681 | None:intergenic |
| GGACTTGGATGACCAGAGGA+GGG | 0.651250 | 3:-5730680 | None:intergenic |
| CAACATACCTGACACCAGCA+TGG | 0.665960 | 3:-5730843 | None:intergenic |
| TACAGGACTTGGATGACCAG+AGG | 0.680767 | 3:-5730684 | None:intergenic |
| CTTGGATGACCAGAGGAGGG+TGG | 0.682495 | 3:-5730677 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 5730037 | 5731514 | 5730037 | ID=MsaG011546 |
| Chr3 | mRNA | 5730037 | 5731514 | 5730037 | ID=MsaT011546.1;Parent=MsaG011546 |
| Chr3 | exon | 5730037 | 5730097 | 5730037 | ID=MsaT011546.1.exon1;Parent=MsaT011546.1 |
| Chr3 | CDS | 5730037 | 5730097 | 5730037 | ID=cds.MsaT011546.1;Parent=MsaT011546.1 |
| Chr3 | exon | 5730618 | 5730857 | 5730618 | ID=MsaT011546.1.exon2;Parent=MsaT011546.1 |
| Chr3 | CDS | 5730618 | 5730857 | 5730618 | ID=cds.MsaT011546.1;Parent=MsaT011546.1 |
| Chr3 | exon | 5731008 | 5731065 | 5731008 | ID=MsaT011546.1.exon3;Parent=MsaT011546.1 |
| Chr3 | CDS | 5731008 | 5731065 | 5731008 | ID=cds.MsaT011546.1;Parent=MsaT011546.1 |
| Chr3 | exon | 5731475 | 5731514 | 5731475 | ID=MsaT011546.1.exon4;Parent=MsaT011546.1 |
| Chr3 | CDS | 5731475 | 5731514 | 5731475 | ID=cds.MsaT011546.1;Parent=MsaT011546.1 |
| Gene Sequence |
| Protein sequence |
