AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011599


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011599 MtrunA17_Chr3g0077451 79.339 242 39 2 35 276 10 240 9.38e-139 390
MsaG011599 MtrunA17_Chr3g0077421 85.238 210 21 1 65 274 1 200 1.11e-130 368
MsaG011599 MtrunA17_Chr3g0077441 85.238 210 21 1 65 274 1 200 1.11e-130 368
MsaG011599 MtrunA17_Chr5g0429931 68.699 246 75 1 31 276 5 248 4.99e-121 346
MsaG011599 MtrunA17_Chr3g0077431 78.774 212 34 2 65 276 1 201 2.57e-117 335
MsaG011599 MtrunA17_Chr3g0077411 63.296 267 92 2 16 276 257 523 1.75e-115 342
MsaG011599 MtrunA17_Chr3g0077411 56.226 265 89 4 2 264 4 243 2.92e-92 282
MsaG011599 MtrunA17_Chr4g0009491 63.436 227 82 1 3 228 206 432 1.32e-95 288
MsaG011599 MtrunA17_Chr4g0009491 53.023 215 77 2 35 249 11 201 1.70e-65 210
MsaG011599 MtrunA17_Chr3g0077361 55.556 261 79 2 16 276 284 507 7.08e-92 280
MsaG011599 MtrunA17_Chr3g0077361 55.472 265 93 3 1 264 20 260 1.78e-87 269
MsaG011599 MtrunA17_Chr3g0077381 60.352 227 82 2 3 228 228 447 1.16e-85 263
MsaG011599 MtrunA17_Chr3g0077381 49.145 234 73 2 35 268 34 221 3.12e-63 205
MsaG011599 MtrunA17_Chr3g0077461 55.294 255 93 6 16 268 232 467 2.58e-84 260
MsaG011599 MtrunA17_Chr3g0077461 53.712 229 74 3 36 264 22 218 5.16e-74 233
MsaG011599 MtrunA17_Chr3g0131891 29.592 294 162 9 20 273 18 306 3.99e-30 115
MsaG011599 MtrunA17_Chr1g0162441 49.495 99 50 0 21 119 7 105 3.89e-29 114
MsaG011599 MtrunA17_Chr1g0162441 36.800 125 66 4 154 271 275 393 9.88e-15 73.6
MsaG011599 MtrunA17_Chr7g0233561 29.808 312 160 10 14 273 11 315 1.09e-28 111
MsaG011599 MtrunA17_Chr4g0033961 42.727 110 63 0 18 127 3 112 2.60e-25 103
MsaG011599 MtrunA17_Chr4g0033961 31.250 192 100 7 104 273 210 391 1.14e-14 73.6
MsaG011599 MtrunA17_Chr3g0103181 30.216 278 157 10 20 269 11 279 3.44e-25 101
MsaG011599 MtrunA17_Chr5g0421921 42.982 114 62 1 161 274 2 112 5.44e-25 96.3
MsaG011599 MtrunA17_Chr5g0421731 42.982 114 62 1 161 274 2 112 5.44e-25 96.3
MsaG011599 MtrunA17_Chr5g0421851 38.415 164 82 4 120 274 34 187 8.05e-25 98.2
MsaG011599 MtrunA17_Chr3g0103171 30.038 263 153 7 36 269 5 265 1.58e-24 99.4
MsaG011599 MtrunA17_Chr8g0390841 25.083 303 166 7 21 271 39 332 3.76e-23 98.6
MsaG011599 MtrunA17_Chr1g0162411 47.778 90 47 0 21 110 7 96 1.49e-22 96.3
MsaG011599 MtrunA17_Chr7g0233521 25.641 273 159 5 37 273 2 266 3.00e-22 93.2
MsaG011599 MtrunA17_Chr1g0163071 27.365 296 145 8 37 271 2 288 1.35e-20 89.4
MsaG011599 MtrunA17_Chr1g0208771 25.952 289 152 8 36 271 12 291 4.80e-20 89.4
MsaG011599 MtrunA17_Chr7g0233531 26.217 267 165 6 37 273 2 266 4.90e-20 87.4
MsaG011599 MtrunA17_Chr1g0154161 26.316 285 168 8 20 269 29 306 7.44e-20 87.4
MsaG011599 MtrunA17_Chr7g0233571 37.624 101 63 0 20 120 9 109 8.14e-20 85.5
MsaG011599 MtrunA17_Chr7g0233541 23.448 290 161 6 37 273 2 283 8.63e-20 87.0
MsaG011599 MtrunA17_Chr5g0419201 36.364 99 63 0 21 119 16 114 9.77e-20 84.7
MsaG011599 MtrunA17_Chr3g0103151 38.462 117 69 2 20 136 11 124 1.15e-19 83.6
MsaG011599 MtrunA17_Chr3g0103191 39.394 99 58 1 21 119 12 108 1.23e-18 82.8
MsaG011599 MtrunA17_Chr1g0163221 24.138 290 172 8 13 263 3 283 9.71e-18 82.0
MsaG011599 MtrunA17_Chr6g0467351 36.538 104 65 1 20 122 14 117 2.22e-17 79.0
MsaG011599 MtrunA17_Chr1g0162421 50.000 58 29 0 35 92 7 64 1.10e-16 72.8
MsaG011599 MtrunA17_Chr4g0067431 26.059 307 165 12 20 271 14 313 6.54e-16 76.3
MsaG011599 MtrunA17_Chr1g0163131 30.328 122 82 2 13 134 10 128 3.17e-14 71.2
MsaG011599 MtrunA17_Chr1g0171611 25.000 260 170 8 19 261 7 258 1.31e-13 69.3
MsaG011599 MtrunA17_Chr1g0163261 23.322 283 182 9 19 271 7 284 5.41e-13 67.8
MsaG011599 MtrunA17_Chr1g0163061 31.731 104 70 1 20 122 14 117 8.37e-13 67.0
MsaG011599 MtrunA17_Chr1g0163101 29.839 124 74 3 20 143 16 126 8.50e-13 65.5
MsaG011599 MtrunA17_Chr3g0103201 28.311 219 125 7 80 269 5 220 3.07e-12 64.7
MsaG011599 MtrunA17_Chr1g0154071 36.842 95 59 1 20 114 27 120 5.98e-12 65.5
MsaG011599 MtrunA17_Chr6g0476181 29.545 132 90 2 37 168 2 130 1.62e-11 61.2
MsaG011599 MtrunA17_Chr1g0163191 29.808 104 71 1 30 133 14 115 1.86e-11 61.2
MsaG011599 MtrunA17_Chr1g0154211 35.870 92 58 1 20 111 22 112 2.76e-11 63.2
MsaG011599 MtrunA17_Chr1g0154101 34.066 91 60 0 20 110 24 114 3.39e-11 63.5
MsaG011599 MtrunA17_Chr1g0154191 35.870 92 59 0 20 111 21 112 4.54e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011599 AT3G18990.1 37.241 145 87 1 21 165 6 146 8.38e-25 101
MsaG011599 AT3G18990.1 35.938 128 70 4 154 275 220 341 9.81e-14 70.9
MsaG011599 AT1G49475.1 38.732 142 85 2 12 153 25 164 1.88e-24 97.4
MsaG011599 AT3G18990.2 38.346 133 78 1 21 153 6 134 2.16e-24 101
MsaG011599 AT3G18990.2 35.659 129 71 4 154 276 220 342 1.50e-13 70.5
MsaG011599 AT3G18960.2 39.316 117 68 1 5 121 17 130 1.18e-22 92.8
MsaG011599 AT3G18960.1 39.316 117 68 1 5 121 17 130 1.64e-22 92.8
MsaG011599 AT4G01580.1 38.793 116 69 1 6 121 17 130 5.45e-22 90.9
MsaG011599 AT3G18960.3 36.957 92 58 0 30 121 2 93 1.99e-16 75.5
MsaG011599 AT1G49480.3 35.938 128 70 4 154 275 105 226 2.96e-14 70.9
MsaG011599 AT1G49480.1 35.938 128 70 4 154 275 105 226 2.96e-14 70.9
MsaG011599 AT1G49480.2 35.938 128 70 4 154 275 105 226 2.96e-14 70.9

Find 54 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTTCCCATTAGAATTT+TGG 0.142530 3:+7687549 None:intergenic
ACTGCTCTCCTTGAGAATTT+TGG 0.150373 3:+7687389 None:intergenic
GATGAGTTTATAACAAGATT+TGG 0.275708 3:-7687973 MsaT011599.1:CDS
TTTGCAGAATACTATTCTAT+AGG 0.313465 3:-7687841 MsaT011599.1:CDS
CATTGCAGATGATGATGATT+TGG 0.324669 3:+7687339 None:intergenic
TTGCAGAATACTATTCTATA+GGG 0.341018 3:-7687840 MsaT011599.1:CDS
TGGAGCTTTATAGGAACATT+TGG 0.361401 3:+7687409 None:intergenic
TTCCTGTGTTTCAAATATGA+AGG 0.394296 3:-7687808 MsaT011599.1:CDS
AATCTTGTTATAAACTCATC+TGG 0.413404 3:+7687976 None:intergenic
TTCAAAACTCAAAGTACAAA+TGG 0.427538 3:-7687727 MsaT011599.1:CDS
GGAATTTACTGTATTTCAAA+GGG 0.428430 3:-7687305 MsaT011599.1:CDS
CGATGGTTGTGATTGGGAAA+TGG 0.431948 3:-7687914 MsaT011599.1:CDS
ATTCTCACTTGTTTCAACCT+TGG 0.452920 3:+7687666 None:intergenic
CTGTTCCCGATGGTTGTGAT+TGG 0.455470 3:-7687921 MsaT011599.1:CDS
GGGAATTTACTGTATTTCAA+AGG 0.463623 3:-7687306 MsaT011599.1:CDS
CATCTGCAATGATAATATCA+AGG 0.481222 3:-7687327 MsaT011599.1:CDS
GTGGCCGAACAGAGCTTCTC+CGG 0.485302 3:+7689299 None:intergenic
AGTGTCACAGTCGTAGAAAA+AGG 0.493455 3:-7687690 MsaT011599.1:CDS
TGTTCCCGATGGTTGTGATT+GGG 0.495072 3:-7687920 MsaT011599.1:CDS
TTGAAACAAGTGAGAATCAT+GGG 0.495409 3:-7687660 MsaT011599.1:CDS
GAGAAGCTCTGTTCGGCCAC+CGG 0.497576 3:-7689296 MsaT011599.1:CDS
AATTCTAATGGGAAATTATG+TGG 0.499901 3:-7687545 MsaT011599.1:intron
CCCCTTCATATTTGAAACAC+AGG 0.508323 3:+7687806 None:intergenic
CATTTCCCAATCACAACCAT+CGG 0.511594 3:+7687915 None:intergenic
TTGCAACAATGCAGCCTCGC+CGG 0.519514 3:-7689318 None:intergenic
GAATACTATTCTATAGGGTA+TGG 0.523564 3:-7687835 MsaT011599.1:CDS
GTTGAAACAAGTGAGAATCA+TGG 0.524886 3:-7687661 MsaT011599.1:CDS
TTAGAATCCTTCTCTGCCGC+CGG 0.528074 3:+7689277 None:intergenic
TCGGCCACCGGCGGCAGAGA+AGG 0.528820 3:-7689284 MsaT011599.1:CDS
TCCTGTGTTTCAAATATGAA+GGG 0.529278 3:-7687807 MsaT011599.1:CDS
AGGGGCAACAATCTGTCTCA+TGG 0.538258 3:-7687286 MsaT011599.1:CDS
TACTAATTGAGTGTCATGAA+TGG 0.550839 3:+7689225 None:intergenic
AATGTGCAGTATGTTAATGC+TGG 0.551027 3:-7687454 MsaT011599.1:intron
CTCGCCGGAGAAGCTCTGTT+CGG 0.553709 3:-7689303 MsaT011599.1:CDS
ATTTCCCAATCACAACCATC+GGG 0.554134 3:+7687916 None:intergenic
ATAAAGCTCCAAAATTCTCA+AGG 0.564100 3:-7687397 MsaT011599.1:CDS
AAGCTCTGTTCGGCCACCGG+CGG 0.575424 3:-7689293 MsaT011599.1:CDS
ATGAATGGGTGAGGGAAGGA+TGG 0.575645 3:+7689240 None:intergenic
GAATCCTTCTCTGCCGCCGG+TGG 0.576638 3:+7689280 None:intergenic
ATCTGCAATGATAATATCAA+GGG 0.582352 3:-7687326 MsaT011599.1:CDS
ACTAATTGAGTGTCATGAAT+GGG 0.585550 3:+7689226 None:intergenic
CCTGTGTTTCAAATATGAAG+GGG 0.591078 3:-7687806 MsaT011599.1:CDS
AAATTCTCAAGGAGAGCAGT+GGG 0.598086 3:-7687386 MsaT011599.1:CDS
AAAATTCTCAAGGAGAGCAG+TGG 0.599450 3:-7687387 MsaT011599.1:CDS
TTGAGTGTCATGAATGGGTG+AGG 0.601105 3:+7689231 None:intergenic
TGAGTGTCATGAATGGGTGA+GGG 0.619523 3:+7689232 None:intergenic
GCTACACTTACTGTTCCCGA+TGG 0.622235 3:-7687931 MsaT011599.1:CDS
TTCATATTTGAAACACAGGA+AGG 0.623348 3:+7687810 None:intergenic
CGAACAGAGCTTCTCCGGCG+AGG 0.626779 3:+7689304 None:intergenic
TGTCATGAATGGGTGAGGGA+AGG 0.626884 3:+7689236 None:intergenic
ACATGCTTCTGAAAGAGCAG+AGG 0.639287 3:-7687614 MsaT011599.1:CDS
GAATTTACTGTATTTCAAAG+GGG 0.669947 3:-7687304 MsaT011599.1:CDS
GAAATGGAACTGAAGAAATG+CGG 0.682734 3:-7687898 MsaT011599.1:CDS
TGAAACAAGTGAGAATCATG+GGG 0.698370 3:-7687659 MsaT011599.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 7687201 7689332 7687201 ID=MsaG011599
Chr3 mRNA 7687201 7689332 7687201 ID=MsaT011599.1;Parent=MsaG011599
Chr3 exon 7687201 7687467 7687201 ID=MsaT011599.1.exon3;Parent=MsaT011599.1
Chr3 CDS 7687201 7687467 7687201 ID=cds.MsaT011599.1;Parent=MsaT011599.1
Chr3 exon 7687546 7688004 7687546 ID=MsaT011599.1.exon2;Parent=MsaT011599.1
Chr3 CDS 7687546 7688004 7687546 ID=cds.MsaT011599.1;Parent=MsaT011599.1
Chr3 exon 7689228 7689332 7689228 ID=MsaT011599.1.exon1;Parent=MsaT011599.1
Chr3 CDS 7689228 7689332 7689228 ID=cds.MsaT011599.1;Parent=MsaT011599.1
Gene Sequence

>MsaT011599.1

ATGCAGCCTCGCCGGAGAAGCTCTGTTCGGCCACCGGCGGCAGAGAAGGATTCTAAACATTTTATGAAAGCCATCCTTCCCTCACCCATTCATGACACTCAATTAAGAATTCCAGATGAGTTTATAACAAGATTTGGACATGAACTCAAGAATGTTGCTACACTTACTGTTCCCGATGGTTGTGATTGGGAAATGGAACTGAAGAAATGCGGCAACGACGTTTACTTTTGCAACAAATGGCAACAGTTTGCAGAATACTATTCTATAGGGTATGGTTCCTTCCTGTGTTTCAAATATGAAGGGGATTCAAAGTTTAGTGTTGTTATATTTGATGTAACTTCTGTTGAGATTTGCTATCCATTCAAAACTCAAAGTACAAATGGAGAAACAAACACAAAGTGTCACAGTCGTAGAAAAAGGTCCAAGGTTGAAACAAGTGAGAATCATGGGGAAACTGTCAAAAGCATCTCTAAACATGCTTCTGAAAGAGCAGAGGAAGCTGCCAAAGAATTTAATCCAAAAAACCCTTATTTTTGTTCCAAAATTCTAATGGGAAATTATGTGTATGTTAATGCTGGTTTTGCTTCAAAGCATCTGAATCCAAATGTTCCTATAAAGCTCCAAAATTCTCAAGGAGAGCAGTGGGAAGTTTCTTGTGCAATGCATGATGCCAAATCATCATCATCTGCAATGATAATATCAAGGGAATTTACTGTATTTCAAAGGGGCAACAATCTGTCTCATGGAGATTATTGTGTGTTTGAGCTGATCAAAAAGAAGCCGGATGTGCTAAAAGTCAAAATGTTTCGTGTGATTGACTATTGCGATTAA

Protein sequence

>MsaT011599.1

MQPRRRSSVRPPAAEKDSKHFMKAILPSPIHDTQLRIPDEFITRFGHELKNVATLTVPDGCDWEMELKKCGNDVYFCNKWQQFAEYYSIGYGSFLCFKYEGDSKFSVVIFDVTSVEICYPFKTQSTNGETNTKCHSRRKRSKVETSENHGETVKSISKHASERAEEAAKEFNPKNPYFCSKILMGNYVYVNAGFASKHLNPNVPIKLQNSQGEQWEVSCAMHDAKSSSSAMIISREFTVFQRGNNLSHGDYCVFELIKKKPDVLKVKMFRVIDYCD*