AlfalfaGEDB | MsaG011600

M. sativa ssp. caerulea / MsaG011600

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011600	AES68281.1	86.528	193	16	1	8	200	36	218	3.24e-119	349

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011600	sp\|Q8L3W1\|VRN1_ARATH	36.765	136	82	1	2	137	15	146	3.99e-22	95.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011600	tr\|G7IYE9\|G7IYE9_MEDTR	86.528	193	16	1	8	200	36	218	1.55e-119	349

TFs/TRs:

Gene ID	Type	Classification
MsaG011600	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001730	MsaG011600	0.804897	2.926434e-49	4.426111e-47
MsaG007615	MsaG011600	0.820096	1.439371e-52	3.173829e-50
MsaG007817	MsaG011600	0.812806	6.061515e-51	1.108989e-48
MsaG008234	MsaG011600	0.823298	2.637258e-53	6.333929e-51
MsaG011600	MsaG013839	0.803724	5.121955e-49	7.539031e-47
MsaG011600	MsaG016209	0.800557	2.280122e-48	3.121902e-46
MsaG011600	MsaG016215	0.830772	4.381236e-55	1.296274e-52
MsaG011600	MsaG017189	0.830354	5.539993e-55	1.619588e-52
MsaG011600	MsaG019491	0.807834	7.083812e-50	1.148206e-47
MsaG011600	MsaG020321	0.804606	3.363966e-49	5.053441e-47
MsaG011600	MsaG027987	0.856346	6.645231e-62	4.508675e-59
MsaG011600	MsaG029141	0.835550	2.868079e-56	9.766750e-54
MsaG011600	MsaG032115	0.803111	6.855277e-49	9.948116e-47
MsaG011600	MsaG033051	0.802180	1.064135e-48	1.511576e-46
MsaG011600	MsaG041800	0.805800	1.897175e-49	2.930276e-47
MsaG011600	MsaG046522	0.801791	1.278329e-48	1.799826e-46
MsaG011600	MsaG000635	0.819310	2.172069e-52	4.691045e-50
MsaG011600	MsaG011183	0.809812	2.686991e-50	4.567882e-48
MsaG011600	MsaG013496	0.831328	3.204671e-55	9.634725e-53
MsaG011600	MsaG036233	0.816442	9.577948e-52	1.920524e-49
MsaG011600	MsaG037508	0.826074	5.891485e-54	1.526756e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011600	MtrunA17_Chr3g0077451	81.865	193	25	1	8	200	11	193	7.71e-114	324
MsaG011600	MtrunA17_Chr3g0077421	85.976	164	13	1	37	200	1	154	2.80e-100	288
MsaG011600	MtrunA17_Chr3g0077441	85.976	164	13	1	37	200	1	154	2.80e-100	288
MsaG011600	MtrunA17_Chr5g0429931	67.839	199	64	0	3	201	5	203	1.75e-96	280
MsaG011600	MtrunA17_Chr3g0077431	81.098	164	21	1	37	200	1	154	1.58e-92	269
MsaG011600	MtrunA17_Chr3g0077411	67.010	194	63	1	8	200	277	470	2.04e-88	269
MsaG011600	MtrunA17_Chr3g0077411	59.140	186	53	2	8	193	40	202	5.80e-70	221
MsaG011600	MtrunA17_Chr4g0009491	64.322	199	71	0	2	200	234	432	1.34e-85	259
MsaG011600	MtrunA17_Chr4g0009491	52.500	200	73	1	2	201	6	183	8.86e-63	200
MsaG011600	MtrunA17_Chr3g0077381	61.307	199	70	1	2	200	256	447	1.74e-76	236
MsaG011600	MtrunA17_Chr3g0077381	54.348	184	62	1	2	185	29	190	9.01e-60	193
MsaG011600	MtrunA17_Chr3g0077461	54.639	194	58	2	8	201	22	185	1.95e-66	211
MsaG011600	MtrunA17_Chr3g0077461	57.292	192	66	4	2	192	246	422	1.27e-64	206
MsaG011600	MtrunA17_Chr3g0077361	54.872	195	65	2	8	201	55	227	3.58e-62	201
MsaG011600	MtrunA17_Chr3g0077361	51.500	200	62	1	2	201	298	462	4.09e-61	198
MsaG011600	MtrunA17_Chr1g0162441	47.778	90	47	0	2	91	16	105	4.97e-26	103
MsaG011600	MtrunA17_Chr4g0033961	40.816	98	58	0	2	99	15	112	7.83e-22	92.0
MsaG011600	MtrunA17_Chr7g0233561	36.585	123	69	2	8	121	33	155	2.00e-20	87.0
MsaG011600	MtrunA17_Chr7g0233521	28.205	195	110	3	9	181	2	188	2.58e-20	86.3
MsaG011600	MtrunA17_Chr3g0103171	31.122	196	104	5	8	178	5	194	7.08e-20	84.7
MsaG011600	MtrunA17_Chr1g0162411	46.914	81	43	0	2	82	16	96	8.35e-20	86.3
MsaG011600	MtrunA17_Chr3g0131891	28.571	217	115	7	9	187	35	249	6.51e-19	83.2
MsaG011600	MtrunA17_Chr5g0419201	39.759	83	50	0	9	91	32	114	1.49e-18	79.7
MsaG011600	MtrunA17_Chr7g0233531	27.835	194	111	3	9	181	2	187	1.38e-17	78.6
MsaG011600	MtrunA17_Chr7g0233541	25.238	210	112	4	9	181	2	203	2.87e-17	78.2
MsaG011600	MtrunA17_Chr3g0103151	37.624	101	62	1	8	108	25	124	5.94e-17	74.7
MsaG011600	MtrunA17_Chr3g0103191	29.310	174	94	4	8	154	25	196	7.42e-17	76.3
MsaG011600	MtrunA17_Chr3g0103181	31.579	171	90	5	8	154	25	192	8.80e-17	76.6
MsaG011600	MtrunA17_Chr1g0162421	49.123	57	29	0	8	64	8	64	1.24e-16	71.2
MsaG011600	MtrunA17_Chr7g0233571	38.095	84	52	0	9	92	26	109	3.25e-16	73.9
MsaG011600	MtrunA17_Chr8g0390841	29.927	137	88	1	2	130	48	184	1.50e-15	74.3
MsaG011600	MtrunA17_Chr1g0163071	40.000	85	51	0	9	93	2	86	2.60e-15	72.8
MsaG011600	MtrunA17_Chr1g0154161	26.168	214	126	5	9	190	46	259	1.13e-14	71.2
MsaG011600	MtrunA17_Chr1g0208771	28.472	144	98	2	8	146	12	155	1.07e-13	68.9
MsaG011600	MtrunA17_Chr6g0467351	35.227	88	56	1	8	94	30	117	5.04e-13	65.5
MsaG011600	MtrunA17_Chr5g0421921	44.262	61	34	0	133	193	2	62	7.36e-13	62.8
MsaG011600	MtrunA17_Chr5g0421731	44.262	61	34	0	133	193	2	62	7.36e-13	62.8
MsaG011600	MtrunA17_Chr5g0421851	36.937	111	54	3	92	193	34	137	1.13e-12	63.9
MsaG011600	MtrunA17_Chr6g0476181	29.545	132	90	2	9	140	2	130	4.79e-12	61.6
MsaG011600	MtrunA17_Chr1g0163131	32.653	98	63	2	9	106	34	128	4.96e-12	63.5
MsaG011600	MtrunA17_Chr1g0154071	29.333	150	94	3	5	145	167	313	9.81e-12	63.2
MsaG011600	MtrunA17_Chr1g0154071	36.782	87	54	1	1	86	34	120	2.23e-11	62.0
MsaG011600	MtrunA17_Chr1g0163221	35.227	88	50	2	9	92	27	111	1.55e-11	62.4
MsaG011600	MtrunA17_Chr1g0154191	40.000	75	45	0	9	83	38	112	2.75e-11	61.6
MsaG011600	MtrunA17_Chr1g0163241	31.868	91	60	1	1	91	15	103	3.78e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG011600	AT3G18990.1	36.765	136	82	1	2	137	15	146	4.06e-23	95.1
MsaG011600	AT3G18990.2	36.765	136	82	1	2	137	15	146	1.01e-22	94.4
MsaG011600	AT1G49475.1	38.983	118	70	2	8	125	49	164	2.83e-20	84.3
MsaG011600	AT3G18960.2	39.535	86	52	0	8	93	45	130	4.40e-17	76.3
MsaG011600	AT3G18960.1	39.535	86	52	0	8	93	45	130	5.65e-17	76.3
MsaG011600	AT3G18960.3	39.535	86	52	0	8	93	8	93	6.29e-17	75.1
MsaG011600	AT4G01580.1	39.535	86	52	0	8	93	45	130	7.00e-17	75.5

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATAATTTCCCATTAGAATTT+TGG	0.142530	3:+7693367	None:intergenic
ACTGCTCTCCTTGAGAATTT+TGG	0.150373	3:+7693207	None:intergenic
GATGAGTTTATAACAAGATT+TGG	0.275708	3:-7693791	MsaT011600.1:CDS
TTCATTCAATGATGATGATT+TGG	0.276761	3:+7693157	None:intergenic
TTTGCAGAATACTATTCTAT+AGG	0.313465	3:-7693659	MsaT011600.1:CDS
TTGCAGAATACTATTCTATA+GGG	0.341018	3:-7693658	MsaT011600.1:CDS
TGGAGCTTTATAGGAACATT+TGG	0.361401	3:+7693227	None:intergenic
TTCCTGTGTTTCAAATATGA+AGG	0.394296	3:-7693626	MsaT011600.1:CDS
AATCTTGTTATAAACTCATC+TGG	0.413404	3:+7693794	None:intergenic
CATGATTCAATTTCGACATT+CGG	0.415053	3:-7694084	MsaT011600.1:intron
TTCAAAACTCAAAGTACAAA+TGG	0.427538	3:-7693545	MsaT011600.1:CDS
CGATGGTTGTGATTGGGAAA+TGG	0.431948	3:-7693732	MsaT011600.1:CDS
ATTCTCACTTGTTTCAACCT+TGG	0.452920	3:+7693484	None:intergenic
CTGTTCCCGATGGTTGTGAT+TGG	0.455470	3:-7693739	MsaT011600.1:CDS
AGTGTCACAGTCGTAGAAAA+AGG	0.493455	3:-7693508	MsaT011600.1:CDS
TGTTCCCGATGGTTGTGATT+GGG	0.495072	3:-7693738	MsaT011600.1:CDS
TTGAAACAAGTGAGAATCAT+GGG	0.495409	3:-7693478	MsaT011600.1:CDS
AATTCTAATGGGAAATTATG+TGG	0.499901	3:-7693363	MsaT011600.1:intron
CCCCTTCATATTTGAAACAC+AGG	0.508323	3:+7693624	None:intergenic
CATTTCCCAATCACAACCAT+CGG	0.511594	3:+7693733	None:intergenic
GAATACTATTCTATAGGGTA+TGG	0.523564	3:-7693653	MsaT011600.1:CDS
GTTGAAACAAGTGAGAATCA+TGG	0.524886	3:-7693479	MsaT011600.1:CDS
TCCTGTGTTTCAAATATGAA+GGG	0.529278	3:-7693625	MsaT011600.1:CDS
AATGTGCAGTATGTTAATGC+TGG	0.551027	3:-7693272	MsaT011600.1:intron
ATTTCCCAATCACAACCATC+GGG	0.554134	3:+7693734	None:intergenic
ATAAAGCTCCAAAATTCTCA+AGG	0.564100	3:-7693215	MsaT011600.1:CDS
CCTGTGTTTCAAATATGAAG+GGG	0.591078	3:-7693624	MsaT011600.1:CDS
AAATTCTCAAGGAGAGCAGT+GGG	0.598086	3:-7693204	MsaT011600.1:CDS
AAAATTCTCAAGGAGAGCAG+TGG	0.599450	3:-7693205	MsaT011600.1:CDS
GCTACACTTACTGTTCCCGA+TGG	0.622235	3:-7693749	MsaT011600.1:CDS
TTCATATTTGAAACACAGGA+AGG	0.623348	3:+7693628	None:intergenic
ACATGCTTCTGAAAGAGCAG+AGG	0.639287	3:-7693432	MsaT011600.1:CDS
GAAATGGAACTGAAGAAATG+CGG	0.682734	3:-7693716	MsaT011600.1:CDS
TGAAACAAGTGAGAATCATG+GGG	0.698370	3:-7693477	MsaT011600.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	7693154	7694105	7693154	ID=MsaG011600
Chr3	mRNA	7693154	7694105	7693154	ID=MsaT011600.1;Parent=MsaG011600
Chr3	exon	7693154	7693285	7693154	ID=MsaT011600.1.exon3;Parent=MsaT011600.1
Chr3	CDS	7693154	7693285	7693154	ID=cds.MsaT011600.1;Parent=MsaT011600.1
Chr3	exon	7693364	7693822	7693364	ID=MsaT011600.1.exon2;Parent=MsaT011600.1
Chr3	CDS	7693364	7693822	7693364	ID=cds.MsaT011600.1;Parent=MsaT011600.1
Chr3	exon	7694085	7694105	7694085	ID=MsaT011600.1.exon1;Parent=MsaT011600.1
Chr3	CDS	7694085	7694105	7694085	ID=cds.MsaT011600.1;Parent=MsaT011600.1

Gene Sequence

>MsaT011600.1

ATGATTCAATTTCGACATTCGAGAATTCCAGATGAGTTTATAACAAGATTTGGACATGAACTCAAGAATGTTGCTACACTTACTGTTCCCGATGGTTGTGATTGGGAAATGGAACTGAAGAAATGCGGCAACGACGTTTACTTTTGCAACAAATGGCAACAGTTTGCAGAATACTATTCTATAGGGTATGGTTCCTTCCTGTGTTTCAAATATGAAGGGGATTCAAAGTTTAGTGTTGTTATATTTGATGTAACTTCTGTTGAGATTTGCTATCCATTCAAAACTCAAAGTACAAATGGAGAAACAAACACAAAGTGTCACAGTCGTAGAAAAAGGTCCAAGGTTGAAACAAGTGAGAATCATGGGGAAACTGTCAAAAGCATCTCTAAACATGCTTCTGAAAGAGCAGAGGAAGCTGCCAAAGAATTTAATCCAAAAAACCCTTATTTTTGTTCCAAAATTCTAATGGGAAATTATGTGTATGTTAATGCTGGTTTTGCTTCAAAGCATCTGAATCCAAATGTTCCTATAAAGCTCCAAAATTCTCAAGGAGAGCAGTGGGAAGTTTCTTGTGCAATGCATGATGCCAAATCATCATCATTGAATGAATAA

Protein sequence

>MsaT011600.1

MIQFRHSRIPDEFITRFGHELKNVATLTVPDGCDWEMELKKCGNDVYFCNKWQQFAEYYSIGYGSFLCFKYEGDSKFSVVIFDVTSVEICYPFKTQSTNGETNTKCHSRRKRSKVETSENHGETVKSISKHASERAEEAAKEFNPKNPYFCSKILMGNYVYVNAGFASKHLNPNVPIKLQNSQGEQWEVSCAMHDAKSSSLNE*